MS007299 (gene) Bitter gourd (TR) v1

Overview
NameMS007299
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionATP-dependent Clp protease proteolytic subunit
Locationscaffold25: 2098178 .. 2102416 (-)
RNA-Seq ExpressionMS007299
SyntenyMS007299
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCACTGGGATGAAGCTGCCCATGGCCATCAGCTTGCAGAAACCCATGAACATGGCGTCTCAATCTTCATCGTCTTCTCTCGCGAGAGGCACCAATTTCAGAACTGTTTGCTGTACGAACGCTACAAGCAAAGCTAAAATACCTTTGCCTCCGATAAACCCGAAGGATCCATTTCTTTCGAAGCTCGCTTCAGTAGCTTCTACCTCACCTGAAACGTTGCTGAACCGGCCTTCCAACTCTGATTCCCCTCCGTATTTGGATTTGTTTGATGCTCCTCAGCTCATGGCTGCACCTGCGCAAGTAAGTTAGCCGTCCCTCGCTTATTTTCATATTACTTGTATGTTCTTACAGGTACTTGTATGAATTTCTGAGAGCAAACGGAGTTTTCTTTAAATCAATTCATATTAATTTGAAGTAAGTTTGTTAGTTAGCTAATGTTGCGAATTTGGTGGTTTTCTTATTGGATAAGGTCGAAAGATCAGTTTCCTACAACGAGCATAGGCCGAGGAGGCCACCGCCGGACTTGCCTTCATTATTACTTAATGGAAGAATTGTTTACATCGGAATGCCTGTAAGTGCTTGAATTAGCGTTAGTCCACTGTTATACGGTTTTACTCCTTAATCGAGAAACTATTCAACTTACCGAAGGGGAGAGTATTACTACGATTTTATTCAATATCGTGTTGTTGAGAAGTTTCATTGAAAGAAGAAGAGAAGCATGTTCTTCGAACTTATGAACAATTGGAATAGTTTTAACTAATGCAAACTGGAAAGGTGCATCTCGTGATAATTGCATATTTTAGACCTTTAGAGGCCTATAGAGTGAAGTTATAGTTCAGGGACGAGTGATGTCAAGCTGATAATGAACCTTGTTGGTTGAAATTGGAAGTGTCGATGAAGTTGTGTGGTGAGTACTTCGGTCTTATGATAGTGCAAGAATATGATTTGTGATTACATCCAGCTGCTGATTTTGAATTTGACAGAAATTCTTTGGAAGTGCTCAATTTGTTTTAGACTGACTTGCGTTTCTTTTTCTTGAGTTGCATAAGCTCTACTGAAATAGCCACTCAAAATATTAGTAATTTTTCTTTTTTCAGGCACAATTGATTCTGAATATTCCATGTGTCGACATTCTGAGAGTTTTTGGCTTCTTCTTATAAATAAGAAAATTTCAGTTTCTTGTGTAATGCACATTGCTACTTTCCTGATAACTCTTCATTCAATCATTTCTACTCTCAGTTGGTTCCAGCTGTGACGGAGCTAGTTATTGCTGAATTGATGTACTTGCAATGGATGGACCCAAAAGAGCCAATATATCTTTATATAAACTCCACAGGAACAACTCGTGATGATGGTGAAACTGTAAGCTTTGAATTTATTCAGCTATATGAAACAAGTGAAATTAAGTTGAAAAGAGCAAGCATTTTTTTCCTCTTGCTTTATACAAGCTTCTGCCCACCGCAGGGTTCTTCAGGGCTTGCCTGCAGACTATAAGTCATGCCCCTTAATCTATGTGTTGACATGAATATTGACTTGTTTGTCTTGACCTGGAGTTCTGTTCTTTCAGATGCTGCTGGATTAAAAAATATATGTGGATGACCAGCATGCTCTTTTGTTTGTGGGTTCTTCAGGGTTTGGTTATTTTATTTGAAAATTGAAAATGAGATCGTAAATTTGAGATTTTACATTTCCGAAAACATGTTTGATTGTAATTCTTAAGAATAGCTTCTTGATCCACAATATGTGAGATTGGGCGTGGGTGTATTTTCTTAAAACAGTAATATCAGAGCATGTTTGGTGAATTATTTGATAAACAACATTTGTTTACTCTTGTGGTGATTATCTCAATAGTAAACTAATAATAAAAACATGCTCAATATTTATGTGGCAAGAGAGTGAATCTATTTTGTGTCATCAGAACACTGTTAACTAAGATGGTCATGCTACTTCTTAGACTCACATGTACCATAGAATGAACAATCTGATAGTATAGAGATGAGTTTTCCGCATGATTATGAAGTCTTTCGATGGAAAAATGTGGGTCTTTCACAAGCTCTGAAGATGGGGTAATATGAGGTGATGAGTGTTTCACAAGATTATGAGATTTGATTATGAAAAGATGTAGATCTTTCACAAGATTATCAAATTTGTGAACGGAGAAGGGATGAGTCTTTAACGAGCTAGCTTATTAATTTGATGGAGGGAAATGGGTCTGAACAAATATGGACGGTTTAGATAAATATTGCGAAATATGGTATTGGAGAGGAGATGGATCTTTCAAGAACTAGCTTACTAATTTGATGGTGGATAATGGGTTTGAACCAGTATGGATGGGTTTTAGCTTGCTGCATAGGTTGGTCCAAGACTGAGATATGTGTATAATAATGTTGGGGATTCGCTTTTCTGAAAAATGCACCTTATATTTCTGTTGATTCTGAAAATGAAAAACCAAATATCTAAAATGATCTTTTGTTTTTCCTGGAAAAGTGGGTTATTATACTAGGTTAACTGATTAATAAAAAAGACTTGCATTATATCTTGTTTGGTTAGTAAGTGTATGCATTCCTTTGATATCCTTTTCCTCAACATACTTAAATTATTTGCCATTCAGGTGGGGATGGAGACAGAGGGTTTTGCTATCTATGATGCTATGATGCAGTTAAAGAATGAGGTATGTTTTCTTGTCAAAACCATTCCGTTCATTCCTACTATTCTTTTGGTGGCAAGATGGTAACCTGATACATTGAGAGTCTTGATATACTCGTTAATATCATAGATTTTTATCTTGGTGTAACTTAACACTGCATCAAGGCTATGGTCTCTCTGGTCATGAGAATTGTTTTCAAAAAGTTGTCACGGTGGTGATATGTCACAAGTGGCTTGAGAAATGACATACTGTTGATTGACCTCTTTTGCTCCAGTAACCTGCATAAACTAATAATTTTAATTTTGTAAAAAATATTGAAAGTAAATAATTTTTAATTTAAACAGTGTCCTTGGAAATAATCATGCTCTGTTATTTGAATCTCTTGCTGATTTTGTGTATTCTGATCCTCTTTTCTTTTATGTACGCAATGGCCCTTACGTAAGGACTATATTGTCCCCACTTTTTTTATGAAATACAAGATGCTCAATAAATAATATGAAACCGATATCTCATACAGATACATACAGTGGCTGTTGGAGCTGCCATAGGTCAAGCTTGTTTATTGCTTGCTGCAGGAAGTCAAGGCAAACGTTTTATGATGCCACATGCCAAAGGTTTGATGCATCTGGCATCTTAATTTCTGTTTTTGACTTGGTACCTAGATTTGCTTATATACAATAATCGAATTCTTTTCCAGCCATGATACAGCAGCCTCGTGTCCCTTCATCTGGTTTGATGCCTGCTAGTGACGTCCTCATTCGTGCAAAAGAGGTCAATATCCTTCGATATCTTTTCAAATATTCGACTTAAATGGCTTCACTAAGATAACGAAATCAATTTCAGGCTTCATAAGTTATTTTGGAGTTGAACTCAATTTTCATGACACTGATTTTTGCTGGAGCTCTTAGCACTTTGATTAAAGAAGTGAAAAAGGGAATTCTATTCAAAATCTCAATCAAGTAATCACAGTTAGGAAATTTTTGCATCCTCATACATTGAGTGAAATGATTCAAAATAATTGGGTAACTTATATCTTGATCCCAAAGATTTTCAATGATTTATATCGTGAAAAATAAAACGTTTTTCAGCCATTCACAACAGCTTTTAGAAGAATACAAGTTGAGCTTTGTATATTTCAACTCGTTTTTCAGGTGATAACAAACAGGGACACACTTGTGAAACTCCTAGCAAAGCACACTGGAAATGTAAGCCTAGCTAGGAAACGTTTATTTCCACTCTTTTTTATGAACTCATGAGATCCATATGATCAACTACCATATTAATTCATTATACAACTTGAAATCTTCTCTCAGTCATTAGAGACAGTGTCTAATGTGATGAGAAGACCCTTCTACATGGACTCGACAAGAGCGAAGGAATTCGGAGTTATCGACAAGGTAAATTAATGCACAATTTTTCTTGGATTATAATGCGCTGTGCATGCATTGTTGCTCATGCCTGTAGATGATAACTCTAATTTGGTGAGTTTGATCCAGATTCTCTGGCGCGGTCAGGAGAAGATCATGGCTGATGTCGCTGCCCCGGAGGAATGGGACAAAGGAGCTGGCATCAAAGTCGCAGACGAATTC

mRNA sequence

ATGGCCACTGGGATGAAGCTGCCCATGGCCATCAGCTTGCAGAAACCCATGAACATGGCGTCTCAATCTTCATCGTCTTCTCTCGCGAGAGGCACCAATTTCAGAACTGTTTGCTGTACGAACGCTACAAGCAAAGCTAAAATACCTTTGCCTCCGATAAACCCGAAGGATCCATTTCTTTCGAAGCTCGCTTCAGTAGCTTCTACCTCACCTGAAACGTTGCTGAACCGGCCTTCCAACTCTGATTCCCCTCCGTATTTGGATTTGTTTGATGCTCCTCAGCTCATGGCTGCACCTGCGCAAGTCGAAAGATCAGTTTCCTACAACGAGCATAGGCCGAGGAGGCCACCGCCGGACTTGCCTTCATTATTACTTAATGGAAGAATTGTTTACATCGGAATGCCTGTAAAAAATTTCAGTTTCTTGTGTAATGCACATTGCTACTTTCCTGATAACTCTTCATTCAATCATTTCTACTCTCAGTTGGTTCCAGCTGTGACGGAGCTAGTTATTGCTGAATTGATGTACTTGCAATGGATGGACCCAAAAGAGCCAATATATCTTTATATAAACTCCACAGGAACAACTCGTGATGATGGTGAAACTATACATACAGTGGCTGTTGGAGCTGCCATAGGTCAAGCTTGTTTATTGCTTGCTGCAGGAAGTCAAGGCAAACGTTTTATGATGCCACATGCCAAAGCCATGATACAGCAGCCTCGTGTCCCTTCATCTGGTTTGATGCCTGCTAGTGACGTCCTCATTCGTGCAAAAGAGGTGATAACAAACAGGGACACACTTGTGAAACTCCTAGCAAAGCACACTGGAAATTCATTAGAGACAGTGTCTAATGTGATGAGAAGACCCTTCTACATGGACTCGACAAGAGCGAAGGAATTCGGAGTTATCGACAAGATTCTCTGGCGCGGTCAGGAGAAGATCATGGCTGATGTCGCTGCCCCGGAGGAATGGGACAAAGGAGCTGGCATCAAAGTCGCAGACGAATTC

Coding sequence (CDS)

ATGGCCACTGGGATGAAGCTGCCCATGGCCATCAGCTTGCAGAAACCCATGAACATGGCGTCTCAATCTTCATCGTCTTCTCTCGCGAGAGGCACCAATTTCAGAACTGTTTGCTGTACGAACGCTACAAGCAAAGCTAAAATACCTTTGCCTCCGATAAACCCGAAGGATCCATTTCTTTCGAAGCTCGCTTCAGTAGCTTCTACCTCACCTGAAACGTTGCTGAACCGGCCTTCCAACTCTGATTCCCCTCCGTATTTGGATTTGTTTGATGCTCCTCAGCTCATGGCTGCACCTGCGCAAGTCGAAAGATCAGTTTCCTACAACGAGCATAGGCCGAGGAGGCCACCGCCGGACTTGCCTTCATTATTACTTAATGGAAGAATTGTTTACATCGGAATGCCTGTAAAAAATTTCAGTTTCTTGTGTAATGCACATTGCTACTTTCCTGATAACTCTTCATTCAATCATTTCTACTCTCAGTTGGTTCCAGCTGTGACGGAGCTAGTTATTGCTGAATTGATGTACTTGCAATGGATGGACCCAAAAGAGCCAATATATCTTTATATAAACTCCACAGGAACAACTCGTGATGATGGTGAAACTATACATACAGTGGCTGTTGGAGCTGCCATAGGTCAAGCTTGTTTATTGCTTGCTGCAGGAAGTCAAGGCAAACGTTTTATGATGCCACATGCCAAAGCCATGATACAGCAGCCTCGTGTCCCTTCATCTGGTTTGATGCCTGCTAGTGACGTCCTCATTCGTGCAAAAGAGGTGATAACAAACAGGGACACACTTGTGAAACTCCTAGCAAAGCACACTGGAAATTCATTAGAGACAGTGTCTAATGTGATGAGAAGACCCTTCTACATGGACTCGACAAGAGCGAAGGAATTCGGAGTTATCGACAAGATTCTCTGGCGCGGTCAGGAGAAGATCATGGCTGATGTCGCTGCCCCGGAGGAATGGGACAAAGGAGCTGGCATCAAAGTCGCAGACGAATTC

Protein sequence

MATGMKLPMAISLQKPMNMASQSSSSSLARGTNFRTVCCTNATSKAKIPLPPINPKDPFLSKLASVASTSPETLLNRPSNSDSPPYLDLFDAPQLMAAPAQVERSVSYNEHRPRRPPPDLPSLLLNGRIVYIGMPVKNFSFLCNAHCYFPDNSSFNHFYSQLVPAVTELVIAELMYLQWMDPKEPIYLYINSTGTTRDDGETIHTVAVGAAIGQACLLLAAGSQGKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHTGNSLETVSNVMRRPFYMDSTRAKEFGVIDKILWRGQEKIMADVAAPEEWDKGAGIKVADEF
Homology
BLAST of MS007299 vs. NCBI nr
Match: XP_022150809.1 (ATP-dependent Clp protease proteolytic subunit-related protein 3, chloroplastic [Momordica charantia] >XP_022150810.1 ATP-dependent Clp protease proteolytic subunit-related protein 3, chloroplastic [Momordica charantia])

HSP 1 Score: 579.7 bits (1493), Expect = 1.6e-161
Identity = 309/356 (86.80%), Postives = 309/356 (86.80%), Query Frame = 0

Query: 1   MATGMKLPMAISLQKPMNMASQSSSSSLARGTNFRTVCCTNATSKAKIPLPPINPKDPFL 60
           MATGMKLPMAISLQKPM MASQSSSSSLARGTNFRTVCCTNATSKAKIPLPPINPKDPFL
Sbjct: 1   MATGMKLPMAISLQKPMAMASQSSSSSLARGTNFRTVCCTNATSKAKIPLPPINPKDPFL 60

Query: 61  SKLASVASTSPETLLNRPSNSDSPPYLDLFDAPQLMAAPAQVERSVSYNEHRPRRPPPDL 120
           SKLASVASTSPETLLNRPSNSDSPPYLDLFDAPQLMAAPAQVERSVSYNEHRPRRPPPDL
Sbjct: 61  SKLASVASTSPETLLNRPSNSDSPPYLDLFDAPQLMAAPAQVERSVSYNEHRPRRPPPDL 120

Query: 121 PSLLLNGRIVYIGMPVKNFSFLCNAHCYFPDNSSFNHFYSQLVPAVTELVIAELMYLQWM 180
           PSLLLNGRIVYIGMP                          LVPAVTELVIAELMYLQWM
Sbjct: 121 PSLLLNGRIVYIGMP--------------------------LVPAVTELVIAELMYLQWM 180

Query: 181 DPKEPIYLYINSTGTTRDDGET--------------------IHTVAVGAAIGQACLLLA 240
           DPKEPIYLYINSTGTTRDDGET                    IHTVAVGAAIGQACLLLA
Sbjct: 181 DPKEPIYLYINSTGTTRDDGETVGMETEGFAIYDAMMQLKNEIHTVAVGAAIGQACLLLA 240

Query: 241 AGSQGKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHTGNSLE 300
           AGSQGKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHTGNSLE
Sbjct: 241 AGSQGKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHTGNSLE 300

Query: 301 TVSNVMRRPFYMDSTRAKEFGVIDKILWRGQEKIMADVAAPEEWDKGAGIKVADEF 337
           TVSNVMRRPFYMDSTRAKEFGVIDKILWRGQEKIMADVAAPEEWDKGAGIKVADEF
Sbjct: 301 TVSNVMRRPFYMDSTRAKEFGVIDKILWRGQEKIMADVAAPEEWDKGAGIKVADEF 330

BLAST of MS007299 vs. NCBI nr
Match: XP_038890491.1 (ATP-dependent Clp protease proteolytic subunit-related protein 3, chloroplastic [Benincasa hispida] >XP_038890492.1 ATP-dependent Clp protease proteolytic subunit-related protein 3, chloroplastic [Benincasa hispida])

HSP 1 Score: 551.2 bits (1419), Expect = 6.1e-153
Identity = 290/356 (81.46%), Postives = 301/356 (84.55%), Query Frame = 0

Query: 1   MATGMKLPMAISLQKPMNMASQSSSSSLARGTNFRTVCCTNATSKAKIPLPPINPKDPFL 60
           MATGMKLPMA++LQKPM MA+ SSSSSL RG NFRT+ CTN TSKAKIPLPPINPKDPFL
Sbjct: 1   MATGMKLPMAVTLQKPMAMAAPSSSSSLPRGINFRTISCTNGTSKAKIPLPPINPKDPFL 60

Query: 61  SKLASVASTSPETLLNRPSNSDSPPYLDLFDAPQLMAAPAQVERSVSYNEHRPRRPPPDL 120
           SKLASVASTSPETLLNRP NSDSPPYLD+FDAPQLMAAPAQVERSVSYNEHRPRRPPPDL
Sbjct: 61  SKLASVASTSPETLLNRPKNSDSPPYLDIFDAPQLMAAPAQVERSVSYNEHRPRRPPPDL 120

Query: 121 PSLLLNGRIVYIGMPVKNFSFLCNAHCYFPDNSSFNHFYSQLVPAVTELVIAELMYLQWM 180
           PSLLL+GRIVYIGMP                          LVPAVTELVIAELMYLQWM
Sbjct: 121 PSLLLHGRIVYIGMP--------------------------LVPAVTELVIAELMYLQWM 180

Query: 181 DPKEPIYLYINSTGTTRDDGET--------------------IHTVAVGAAIGQACLLLA 240
           DPKEPIYLYINSTGTTRDDGET                    IHTVAVGAAIGQACLLLA
Sbjct: 181 DPKEPIYLYINSTGTTRDDGETVGMETEGFAIYDAMMQLKNEIHTVAVGAAIGQACLLLA 240

Query: 241 AGSQGKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHTGNSLE 300
           AGS+GKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHTGN +E
Sbjct: 241 AGSEGKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHTGNPIE 300

Query: 301 TVSNVMRRPFYMDSTRAKEFGVIDKILWRGQEKIMADVAAPEEWDKGAGIKVADEF 337
           TVSNVM+RPFYMD+T+AKEFGVIDKILWRGQEKIMADVAAPEEWDKGAGIKV DEF
Sbjct: 301 TVSNVMQRPFYMDATKAKEFGVIDKILWRGQEKIMADVAAPEEWDKGAGIKVVDEF 330

BLAST of MS007299 vs. NCBI nr
Match: XP_008454159.1 (PREDICTED: ATP-dependent Clp protease proteolytic subunit-related protein 3, chloroplastic [Cucumis melo] >XP_008454167.1 PREDICTED: ATP-dependent Clp protease proteolytic subunit-related protein 3, chloroplastic [Cucumis melo] >KAA0048029.1 ATP-dependent Clp protease proteolytic subunit-related protein 3 [Cucumis melo var. makuwa])

HSP 1 Score: 525.8 bits (1353), Expect = 2.8e-145
Identity = 277/357 (77.59%), Postives = 297/357 (83.19%), Query Frame = 0

Query: 1   MATGMKLPMAISLQKP--MNMASQSSSSSLARGTNFRTVCCTNATSKAKIPLPPINPKDP 60
           MA GMKLPMA++LQKP  M MA+ SSSSSL R  NFRTV C+NATSKAKIP+PPINPKDP
Sbjct: 1   MAIGMKLPMAVTLQKPMAMAMAAPSSSSSLHRAINFRTVSCSNATSKAKIPMPPINPKDP 60

Query: 61  FLSKLASVASTSPETLLNRPSNSDSPPYLDLFDAPQLMAAPAQVERSVSYNEHRPRRPPP 120
           FLSKLASVASTSPETLLNRP NSDSPPYLD+FDAP+LMAAPAQVERSVSYNEHRPRRPPP
Sbjct: 61  FLSKLASVASTSPETLLNRPENSDSPPYLDIFDAPRLMAAPAQVERSVSYNEHRPRRPPP 120

Query: 121 DLPSLLLNGRIVYIGMPVKNFSFLCNAHCYFPDNSSFNHFYSQLVPAVTELVIAELMYLQ 180
           DLPSLLL+GRIVYIGMP                          LVPAVTEL++AELMYLQ
Sbjct: 121 DLPSLLLHGRIVYIGMP--------------------------LVPAVTELIVAELMYLQ 180

Query: 181 WMDPKEPIYLYINSTGTTRDDGET--------------------IHTVAVGAAIGQACLL 240
           WMDPKEPIY+YINSTGTTRDDGET                    IHTVAVGAAIGQACLL
Sbjct: 181 WMDPKEPIYIYINSTGTTRDDGETIGMETEGFAIYDAMMQLKNEIHTVAVGAAIGQACLL 240

Query: 241 LAAGSQGKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHTGNS 300
           LAAGS+G+R+MMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHTGNS
Sbjct: 241 LAAGSEGRRYMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHTGNS 300

Query: 301 LETVSNVMRRPFYMDSTRAKEFGVIDKILWRGQEKIMADVAAPEEWDKGAGIKVADE 336
           +ETVSNVM+RPFYMD+T AKEFGV+DKILWRGQEKIM+ V APE+WDKGAGIKV DE
Sbjct: 301 VETVSNVMKRPFYMDATLAKEFGVVDKILWRGQEKIMSAVVAPEDWDKGAGIKVVDE 331

BLAST of MS007299 vs. NCBI nr
Match: XP_023545293.1 (ATP-dependent Clp protease proteolytic subunit-related protein 3, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023545294.1 ATP-dependent Clp protease proteolytic subunit-related protein 3, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023545295.1 ATP-dependent Clp protease proteolytic subunit-related protein 3, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 516.2 bits (1328), Expect = 2.2e-142
Identity = 271/355 (76.34%), Postives = 292/355 (82.25%), Query Frame = 0

Query: 1   MATGMKLPMAISLQKPMNMASQSSSSSLARGTNFRTVCCTNATSKAKIPLPPINPKDPFL 60
           MATGMKLPMA+SLQKPM MA  SSSS L RG NFRT C  NATSKAKIPLPPINPKDPFL
Sbjct: 1   MATGMKLPMAVSLQKPMAMAVPSSSSRLPRGINFRTCCSMNATSKAKIPLPPINPKDPFL 60

Query: 61  SKLASVASTSPETLLNRPSNSDSPPYLDLFDAPQLMAAPAQVERSVSYNEHRPRRPPPDL 120
           SKLASVASTSPETLL+RP NSDS PYLD+FD+PQLMAAPAQVERSVSYNEHR RRPPPDL
Sbjct: 61  SKLASVASTSPETLLSRPGNSDSLPYLDIFDSPQLMAAPAQVERSVSYNEHRSRRPPPDL 120

Query: 121 PSLLLNGRIVYIGMPVKNFSFLCNAHCYFPDNSSFNHFYSQLVPAVTELVIAELMYLQWM 180
           PSLLL+GRIVYIGMP                          LVPAVTELVIA+LMYLQWM
Sbjct: 121 PSLLLHGRIVYIGMP--------------------------LVPAVTELVIAQLMYLQWM 180

Query: 181 DPKEPIYLYINSTGTTRDDGET--------------------IHTVAVGAAIGQACLLLA 240
           DPKEPIY+YINS+GTTRDDGET                    IHT+ VGAAIGQACLLLA
Sbjct: 181 DPKEPIYIYINSSGTTRDDGETVAMETEGFAIYDAMMQLKNEIHTLTVGAAIGQACLLLA 240

Query: 241 AGSQGKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHTGNSLE 300
           AG++GKRFMMPH+KAMIQQPRVPSSGLMPASD+LIRAKEVITNRDTLV+LLAKHTGNSLE
Sbjct: 241 AGTKGKRFMMPHSKAMIQQPRVPSSGLMPASDILIRAKEVITNRDTLVELLAKHTGNSLE 300

Query: 301 TVSNVMRRPFYMDSTRAKEFGVIDKILWRGQEKIMADVAAPEEWDKGAGIKVADE 336
           TV+N M+RPFYMD+ +AKEFGVIDKILWRGQEKIMA+VA+PE+WDKGAGIK  DE
Sbjct: 301 TVANAMKRPFYMDAIKAKEFGVIDKILWRGQEKIMAEVASPEDWDKGAGIKAVDE 329

BLAST of MS007299 vs. NCBI nr
Match: XP_022946246.1 (ATP-dependent Clp protease proteolytic subunit-related protein 3, chloroplastic [Cucurbita moschata] >XP_022946247.1 ATP-dependent Clp protease proteolytic subunit-related protein 3, chloroplastic [Cucurbita moschata])

HSP 1 Score: 515.4 bits (1326), Expect = 3.7e-142
Identity = 271/355 (76.34%), Postives = 292/355 (82.25%), Query Frame = 0

Query: 1   MATGMKLPMAISLQKPMNMASQSSSSSLARGTNFRTVCCTNATSKAKIPLPPINPKDPFL 60
           MATGMKLPMA+SLQKPM MA  SSSS L RG NFRT C  NATSKAKIPLPPINPKDPFL
Sbjct: 1   MATGMKLPMAVSLQKPMAMAVPSSSSRLPRGINFRTCCSMNATSKAKIPLPPINPKDPFL 60

Query: 61  SKLASVASTSPETLLNRPSNSDSPPYLDLFDAPQLMAAPAQVERSVSYNEHRPRRPPPDL 120
           SKLASVASTSPETLL+RP NSDS PYLD+FD+PQLMAAPAQVERSVSYNEHR RRPPPDL
Sbjct: 61  SKLASVASTSPETLLSRPGNSDSLPYLDIFDSPQLMAAPAQVERSVSYNEHRSRRPPPDL 120

Query: 121 PSLLLNGRIVYIGMPVKNFSFLCNAHCYFPDNSSFNHFYSQLVPAVTELVIAELMYLQWM 180
           PSLLL+GRIVYIGMP                          LVPAVTELVIA+LMYLQWM
Sbjct: 121 PSLLLHGRIVYIGMP--------------------------LVPAVTELVIAQLMYLQWM 180

Query: 181 DPKEPIYLYINSTGTTRDDGET--------------------IHTVAVGAAIGQACLLLA 240
           DPKEPIY+YINS+GTTRDDGET                    IHT+ VGAAIGQACLLLA
Sbjct: 181 DPKEPIYIYINSSGTTRDDGETVAMETEGFAIYDAMMQLKNEIHTLTVGAAIGQACLLLA 240

Query: 241 AGSQGKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHTGNSLE 300
           AG++G+RFMMPH+KAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLV+LLAKHTGNSLE
Sbjct: 241 AGTKGRRFMMPHSKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVELLAKHTGNSLE 300

Query: 301 TVSNVMRRPFYMDSTRAKEFGVIDKILWRGQEKIMADVAAPEEWDKGAGIKVADE 336
           TV+N M+RPFYMD+ +AKEFGVIDKILWRGQEKIMA+VA+PE+WDKGAGIK  DE
Sbjct: 301 TVANAMKRPFYMDAIKAKEFGVIDKILWRGQEKIMAEVASPEDWDKGAGIKAVDE 329

BLAST of MS007299 vs. ExPASy Swiss-Prot
Match: Q8L770 (ATP-dependent Clp protease proteolytic subunit-related protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CLPR3 PE=1 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 1.0e-118
Identity = 232/353 (65.72%), Postives = 264/353 (74.79%), Query Frame = 0

Query: 9   MAISLQKPMNMASQSSSS-----SLARGTNFRTVCCT-----NATSKAKIPLPPINPKDP 68
           MA  LQ  MN     SSS      L+  ++ R    T      A + AKIP+PPINPKDP
Sbjct: 1   MASCLQASMNSLLPRSSSFSPHPPLSSNSSGRRNLKTFRYAFRAKASAKIPMPPINPKDP 60

Query: 69  FLSKLASVASTSPETLLNRPSNSDSPPYLDLFDAPQLMAAPAQVERSVSYNEHRPRRPPP 128
           FLS LAS+A+ SPE LLNRP N+D PPYLD+FD+PQLM++PAQVERSV+YNEHRPR PPP
Sbjct: 61  FLSTLASIAANSPEKLLNRPVNADVPPYLDIFDSPQLMSSPAQVERSVAYNEHRPRTPPP 120

Query: 129 DLPSLLLNGRIVYIGMPVKNFSFLCNAHCYFPDNSSFNHFYSQLVPAVTELVIAELMYLQ 188
           DLPS+LL+GRIVYIGMP                          LVPAVTELV+AELMYLQ
Sbjct: 121 DLPSMLLDGRIVYIGMP--------------------------LVPAVTELVVAELMYLQ 180

Query: 189 WMDPKEPIYLYINSTGTTRDDGET--------------------IHTVAVGAAIGQACLL 248
           W+DPKEPIY+YINSTGTTRDDGET                    +HTV VGAAIGQACLL
Sbjct: 181 WLDPKEPIYIYINSTGTTRDDGETVGMESEGFAIYDSLMQLKNEVHTVCVGAAIGQACLL 240

Query: 249 LAAGSQGKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHTGNS 308
           L+AG++GKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRD LV+LL+KHTGNS
Sbjct: 241 LSAGTKGKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDILVELLSKHTGNS 300

Query: 309 LETVSNVMRRPFYMDSTRAKEFGVIDKILWRGQEKIMADVAAPEEWDKGAGIK 332
           +ETV+NVMRRP+YMD+ +AKEFGVID+ILWRGQEKI+ADV   EE+DK AGIK
Sbjct: 301 VETVANVMRRPYYMDAPKAKEFGVIDRILWRGQEKIIADVVPSEEFDKNAGIK 327

BLAST of MS007299 vs. ExPASy Swiss-Prot
Match: P74466 (Putative ATP-dependent Clp protease proteolytic subunit-like OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=clpR PE=3 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 2.1e-34
Identity = 84/224 (37.50%), Postives = 132/224 (58.93%), Query Frame = 0

Query: 104 RSVSYNEHRPRRPPPDLPSLLLNGRIVYIGMPVKNFSFLCNAHCYFPDNSSFNHFYSQLV 163
           +S  Y +   + PPPDL SLLL  RIVY+GMP+                 S +    Q+ 
Sbjct: 7   QSSYYGDMAFKTPPPDLESLLLKERIVYLGMPL----------------FSSDEVKQQVG 66

Query: 164 PAVTELVIAELMYLQWMDPKEPIYLYINSTGTTRDDGET--------------------I 223
             VT+L+IA+L+YLQ+ DP +PIY YINSTGT+   G+                     +
Sbjct: 67  IDVTQLIIAQLLYLQFDDPDKPIYFYINSTGTSWYTGDAVGFETEAFAICDTLNYIKPPV 126

Query: 224 HTVAVGAAIGQACLLLAAGSQGKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITN 283
           HT+ +G A+G A ++L++G++G R  +PHA  ++ Q R  + G   A+D+ IRAKEVI+N
Sbjct: 127 HTICIGQAMGTAAMILSSGTKGYRASLPHATIVLNQNRTGAQG--QATDIQIRAKEVISN 186

Query: 284 RDTLVKLLAKHTGNSLETVSNVMRRPFYMDSTRAKEFGVIDKIL 308
           + T++++L+ +TG + E ++  M R FY+   +AKE+G+ID++L
Sbjct: 187 KQTMLEILSLNTGQTQEKLAKDMDRTFYLTPAQAKEYGLIDRVL 212

BLAST of MS007299 vs. ExPASy Swiss-Prot
Match: Q9L4P4 (Putative ATP-dependent Clp protease proteolytic subunit-like OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=clpR PE=3 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 1.3e-33
Identity = 84/232 (36.21%), Postives = 130/232 (56.03%), Query Frame = 0

Query: 96  MAAPAQVERSVSYNEHRPRRPPPDLPSLLLNGRIVYIGMPVKNFSFLCNAHCYFPDNSSF 155
           M    Q  ++  Y +   R PPPDLPSLLL  RI+Y+GMP+                 S 
Sbjct: 1   MLESIQAVQAPYYGDVSYRTPPPDLPSLLLKERIIYLGMPL----------------FSS 60

Query: 156 NHFYSQLVPAVTELVIAELMYLQWMDPKEPIYLYINSTGTTRDDGETI------------ 215
           +    Q+   VTEL+IA+L+YL++ +P++PIY YINSTGT+   G+ I            
Sbjct: 61  DDVKRQVGFDVTELIIAQLLYLEFDNPEKPIYFYINSTGTSWYTGDAIGYETEAFAICDT 120

Query: 216 --------HTVAVGAAIGQACLLLAAGSQGKRFMMPHAKAMIQQPRVPSSGLMPASDVLI 275
                   HT+ +G A+G A ++L+ G+ G R  +PHA  ++ QPR  + G   ASD+ I
Sbjct: 121 MRYIKPPVHTICIGQAMGTAAMILSGGTPGNRASLPHATIVLNQPRTGAQG--QASDIQI 180

Query: 276 RAKEVITNRDTLVKLLAKHTGNSLETVSNVMRRPFYMDSTRAKEFGVIDKIL 308
           RAKEV+ N+ T++++ A++TG   + ++    R  YM   +A E+G+ID++L
Sbjct: 181 RAKEVLANKRTMLEIFARNTGQDPDRLARDTDRMLYMTPAQAVEYGLIDRVL 214

BLAST of MS007299 vs. ExPASy Swiss-Prot
Match: Q8LB10 (ATP-dependent Clp protease proteolytic subunit-related protein 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CLPR4 PE=1 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.8e-30
Identity = 85/213 (39.91%), Postives = 115/213 (53.99%), Query Frame = 0

Query: 115 RPPPDLPSLLLNGRIVYIGMPVKNFSFLCNAHCYFPDNSSFNHFYSQLVPAVTELVIAEL 174
           +PPPDL S L   RIVY+GM                           LVP+VTEL++AE 
Sbjct: 101 QPPPDLASYLFKNRIVYLGM--------------------------SLVPSVTELILAEF 160

Query: 175 MYLQWMDPKEPIYLYINSTGTTRD------DGET-------------IHTVAVGAAIGQA 234
           +YLQ+ D ++PIYLYINSTGTT++      D E              I T+ VG A G+A
Sbjct: 161 LYLQYEDEEKPIYLYINSTGTTKNGEKLGYDTEAFAIYDVMGYVKPPIFTLCVGNAWGEA 220

Query: 235 CLLLAAGSQGKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHT 294
            LLL AG++G R  +P +  MI+QP     G   A+DV I  KE+   +  +VKL +KH 
Sbjct: 221 ALLLTAGAKGNRSALPSSTIMIKQPIARFQG--QATDVEIARKEIKHIKTEMVKLYSKHI 280

Query: 295 GNSLETVSNVMRRPFYMDSTRAKEFGVIDKILW 309
           G S E +   M+RP Y   T A E+G+IDK+++
Sbjct: 281 GKSPEQIEADMKRPKYFSPTEAVEYGIIDKVVY 285

BLAST of MS007299 vs. ExPASy Swiss-Prot
Match: Q9XJ35 (ATP-dependent Clp protease proteolytic subunit-related protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CLPR1 PE=1 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 2.3e-30
Identity = 82/216 (37.96%), Postives = 118/216 (54.63%), Query Frame = 0

Query: 112 RPRRPPPDLPSLLLNGRIVYIGMPVKNFSFLCNAHCYFPDNSSFNHFYSQLVPAVTELVI 171
           RPR  PPDLPSLLL+ RI Y+GMP+                          VPAVTEL++
Sbjct: 164 RPRTAPPDLPSLLLDARICYLGMPI--------------------------VPAVTELLV 223

Query: 172 AELMYLQWMDPKEPIYLYINSTGTTRDDGET--------------------IHTVAVGAA 231
           A+ M+L + +P +PIYLYINS GT  +  ET                    ++T+  G A
Sbjct: 224 AQFMWLDYDNPTKPIYLYINSPGTQNEKMETVGSETEAYAIADTISYCKSDVYTINCGMA 283

Query: 232 IGQACLLLAAGSQGKRFMMPHAKAMIQQPRV-PSSGLMPASDVLIRAKEVITNRDTLVKL 291
            GQA +LL+ G +G R + PH+   +  P+V  SSG   A D+ I+AKE+  N +  ++L
Sbjct: 284 FGQAAMLLSLGKKGYRAVQPHSSTKLYLPKVNRSSG--AAIDMWIKAKELDANTEYYIEL 343

Query: 292 LAKHTGNSLETVSNVMRRPFYMDSTRAKEFGVIDKI 307
           LAK TG S E ++  ++RP Y+ +  A ++G+ DKI
Sbjct: 344 LAKGTGKSKEQINEDIKRPKYLQAQAAIDYGIADKI 351

BLAST of MS007299 vs. ExPASy TrEMBL
Match: A0A6J1D9I5 (ATP-dependent Clp protease proteolytic subunit OS=Momordica charantia OX=3673 GN=LOC111018868 PE=3 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 7.8e-162
Identity = 309/356 (86.80%), Postives = 309/356 (86.80%), Query Frame = 0

Query: 1   MATGMKLPMAISLQKPMNMASQSSSSSLARGTNFRTVCCTNATSKAKIPLPPINPKDPFL 60
           MATGMKLPMAISLQKPM MASQSSSSSLARGTNFRTVCCTNATSKAKIPLPPINPKDPFL
Sbjct: 1   MATGMKLPMAISLQKPMAMASQSSSSSLARGTNFRTVCCTNATSKAKIPLPPINPKDPFL 60

Query: 61  SKLASVASTSPETLLNRPSNSDSPPYLDLFDAPQLMAAPAQVERSVSYNEHRPRRPPPDL 120
           SKLASVASTSPETLLNRPSNSDSPPYLDLFDAPQLMAAPAQVERSVSYNEHRPRRPPPDL
Sbjct: 61  SKLASVASTSPETLLNRPSNSDSPPYLDLFDAPQLMAAPAQVERSVSYNEHRPRRPPPDL 120

Query: 121 PSLLLNGRIVYIGMPVKNFSFLCNAHCYFPDNSSFNHFYSQLVPAVTELVIAELMYLQWM 180
           PSLLLNGRIVYIGMP                          LVPAVTELVIAELMYLQWM
Sbjct: 121 PSLLLNGRIVYIGMP--------------------------LVPAVTELVIAELMYLQWM 180

Query: 181 DPKEPIYLYINSTGTTRDDGET--------------------IHTVAVGAAIGQACLLLA 240
           DPKEPIYLYINSTGTTRDDGET                    IHTVAVGAAIGQACLLLA
Sbjct: 181 DPKEPIYLYINSTGTTRDDGETVGMETEGFAIYDAMMQLKNEIHTVAVGAAIGQACLLLA 240

Query: 241 AGSQGKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHTGNSLE 300
           AGSQGKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHTGNSLE
Sbjct: 241 AGSQGKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHTGNSLE 300

Query: 301 TVSNVMRRPFYMDSTRAKEFGVIDKILWRGQEKIMADVAAPEEWDKGAGIKVADEF 337
           TVSNVMRRPFYMDSTRAKEFGVIDKILWRGQEKIMADVAAPEEWDKGAGIKVADEF
Sbjct: 301 TVSNVMRRPFYMDSTRAKEFGVIDKILWRGQEKIMADVAAPEEWDKGAGIKVADEF 330

BLAST of MS007299 vs. ExPASy TrEMBL
Match: A0A1S3BXZ3 (ATP-dependent Clp protease proteolytic subunit OS=Cucumis melo OX=3656 GN=LOC103494643 PE=3 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 1.3e-145
Identity = 277/357 (77.59%), Postives = 297/357 (83.19%), Query Frame = 0

Query: 1   MATGMKLPMAISLQKP--MNMASQSSSSSLARGTNFRTVCCTNATSKAKIPLPPINPKDP 60
           MA GMKLPMA++LQKP  M MA+ SSSSSL R  NFRTV C+NATSKAKIP+PPINPKDP
Sbjct: 1   MAIGMKLPMAVTLQKPMAMAMAAPSSSSSLHRAINFRTVSCSNATSKAKIPMPPINPKDP 60

Query: 61  FLSKLASVASTSPETLLNRPSNSDSPPYLDLFDAPQLMAAPAQVERSVSYNEHRPRRPPP 120
           FLSKLASVASTSPETLLNRP NSDSPPYLD+FDAP+LMAAPAQVERSVSYNEHRPRRPPP
Sbjct: 61  FLSKLASVASTSPETLLNRPENSDSPPYLDIFDAPRLMAAPAQVERSVSYNEHRPRRPPP 120

Query: 121 DLPSLLLNGRIVYIGMPVKNFSFLCNAHCYFPDNSSFNHFYSQLVPAVTELVIAELMYLQ 180
           DLPSLLL+GRIVYIGMP                          LVPAVTEL++AELMYLQ
Sbjct: 121 DLPSLLLHGRIVYIGMP--------------------------LVPAVTELIVAELMYLQ 180

Query: 181 WMDPKEPIYLYINSTGTTRDDGET--------------------IHTVAVGAAIGQACLL 240
           WMDPKEPIY+YINSTGTTRDDGET                    IHTVAVGAAIGQACLL
Sbjct: 181 WMDPKEPIYIYINSTGTTRDDGETIGMETEGFAIYDAMMQLKNEIHTVAVGAAIGQACLL 240

Query: 241 LAAGSQGKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHTGNS 300
           LAAGS+G+R+MMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHTGNS
Sbjct: 241 LAAGSEGRRYMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHTGNS 300

Query: 301 LETVSNVMRRPFYMDSTRAKEFGVIDKILWRGQEKIMADVAAPEEWDKGAGIKVADE 336
           +ETVSNVM+RPFYMD+T AKEFGV+DKILWRGQEKIM+ V APE+WDKGAGIKV DE
Sbjct: 301 VETVSNVMKRPFYMDATLAKEFGVVDKILWRGQEKIMSAVVAPEDWDKGAGIKVVDE 331

BLAST of MS007299 vs. ExPASy TrEMBL
Match: A0A5A7U3K5 (ATP-dependent Clp protease proteolytic subunit OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G00970 PE=3 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 1.3e-145
Identity = 277/357 (77.59%), Postives = 297/357 (83.19%), Query Frame = 0

Query: 1   MATGMKLPMAISLQKP--MNMASQSSSSSLARGTNFRTVCCTNATSKAKIPLPPINPKDP 60
           MA GMKLPMA++LQKP  M MA+ SSSSSL R  NFRTV C+NATSKAKIP+PPINPKDP
Sbjct: 1   MAIGMKLPMAVTLQKPMAMAMAAPSSSSSLHRAINFRTVSCSNATSKAKIPMPPINPKDP 60

Query: 61  FLSKLASVASTSPETLLNRPSNSDSPPYLDLFDAPQLMAAPAQVERSVSYNEHRPRRPPP 120
           FLSKLASVASTSPETLLNRP NSDSPPYLD+FDAP+LMAAPAQVERSVSYNEHRPRRPPP
Sbjct: 61  FLSKLASVASTSPETLLNRPENSDSPPYLDIFDAPRLMAAPAQVERSVSYNEHRPRRPPP 120

Query: 121 DLPSLLLNGRIVYIGMPVKNFSFLCNAHCYFPDNSSFNHFYSQLVPAVTELVIAELMYLQ 180
           DLPSLLL+GRIVYIGMP                          LVPAVTEL++AELMYLQ
Sbjct: 121 DLPSLLLHGRIVYIGMP--------------------------LVPAVTELIVAELMYLQ 180

Query: 181 WMDPKEPIYLYINSTGTTRDDGET--------------------IHTVAVGAAIGQACLL 240
           WMDPKEPIY+YINSTGTTRDDGET                    IHTVAVGAAIGQACLL
Sbjct: 181 WMDPKEPIYIYINSTGTTRDDGETIGMETEGFAIYDAMMQLKNEIHTVAVGAAIGQACLL 240

Query: 241 LAAGSQGKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHTGNS 300
           LAAGS+G+R+MMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHTGNS
Sbjct: 241 LAAGSEGRRYMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHTGNS 300

Query: 301 LETVSNVMRRPFYMDSTRAKEFGVIDKILWRGQEKIMADVAAPEEWDKGAGIKVADE 336
           +ETVSNVM+RPFYMD+T AKEFGV+DKILWRGQEKIM+ V APE+WDKGAGIKV DE
Sbjct: 301 VETVSNVMKRPFYMDATLAKEFGVVDKILWRGQEKIMSAVVAPEDWDKGAGIKVVDE 331

BLAST of MS007299 vs. ExPASy TrEMBL
Match: A0A6J1G379 (ATP-dependent Clp protease proteolytic subunit OS=Cucurbita moschata OX=3662 GN=LOC111450387 PE=3 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 1.8e-142
Identity = 271/355 (76.34%), Postives = 292/355 (82.25%), Query Frame = 0

Query: 1   MATGMKLPMAISLQKPMNMASQSSSSSLARGTNFRTVCCTNATSKAKIPLPPINPKDPFL 60
           MATGMKLPMA+SLQKPM MA  SSSS L RG NFRT C  NATSKAKIPLPPINPKDPFL
Sbjct: 1   MATGMKLPMAVSLQKPMAMAVPSSSSRLPRGINFRTCCSMNATSKAKIPLPPINPKDPFL 60

Query: 61  SKLASVASTSPETLLNRPSNSDSPPYLDLFDAPQLMAAPAQVERSVSYNEHRPRRPPPDL 120
           SKLASVASTSPETLL+RP NSDS PYLD+FD+PQLMAAPAQVERSVSYNEHR RRPPPDL
Sbjct: 61  SKLASVASTSPETLLSRPGNSDSLPYLDIFDSPQLMAAPAQVERSVSYNEHRSRRPPPDL 120

Query: 121 PSLLLNGRIVYIGMPVKNFSFLCNAHCYFPDNSSFNHFYSQLVPAVTELVIAELMYLQWM 180
           PSLLL+GRIVYIGMP                          LVPAVTELVIA+LMYLQWM
Sbjct: 121 PSLLLHGRIVYIGMP--------------------------LVPAVTELVIAQLMYLQWM 180

Query: 181 DPKEPIYLYINSTGTTRDDGET--------------------IHTVAVGAAIGQACLLLA 240
           DPKEPIY+YINS+GTTRDDGET                    IHT+ VGAAIGQACLLLA
Sbjct: 181 DPKEPIYIYINSSGTTRDDGETVAMETEGFAIYDAMMQLKNEIHTLTVGAAIGQACLLLA 240

Query: 241 AGSQGKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHTGNSLE 300
           AG++G+RFMMPH+KAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLV+LLAKHTGNSLE
Sbjct: 241 AGTKGRRFMMPHSKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVELLAKHTGNSLE 300

Query: 301 TVSNVMRRPFYMDSTRAKEFGVIDKILWRGQEKIMADVAAPEEWDKGAGIKVADE 336
           TV+N M+RPFYMD+ +AKEFGVIDKILWRGQEKIMA+VA+PE+WDKGAGIK  DE
Sbjct: 301 TVANAMKRPFYMDAIKAKEFGVIDKILWRGQEKIMAEVASPEDWDKGAGIKAVDE 329

BLAST of MS007299 vs. ExPASy TrEMBL
Match: A0A6J1KFX9 (ATP-dependent Clp protease proteolytic subunit OS=Cucurbita maxima OX=3661 GN=LOC111493541 PE=3 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 1.9e-139
Identity = 265/355 (74.65%), Postives = 290/355 (81.69%), Query Frame = 0

Query: 1   MATGMKLPMAISLQKPMNMASQSSSSSLARGTNFRTVCCTNATSKAKIPLPPINPKDPFL 60
           MATGMKLPMA+SLQKPM MA  SSSS L R  NF T C  +ATSKAKIPLPPINPKDPFL
Sbjct: 1   MATGMKLPMAVSLQKPMAMAVPSSSSRLPRRINFSTCCSMSATSKAKIPLPPINPKDPFL 60

Query: 61  SKLASVASTSPETLLNRPSNSDSPPYLDLFDAPQLMAAPAQVERSVSYNEHRPRRPPPDL 120
           +KLASVASTSPETLL+RP NSDS PYLD+FD+PQLMAAPAQVERSVSYNEHR RRPPPDL
Sbjct: 61  AKLASVASTSPETLLSRPGNSDSLPYLDIFDSPQLMAAPAQVERSVSYNEHRSRRPPPDL 120

Query: 121 PSLLLNGRIVYIGMPVKNFSFLCNAHCYFPDNSSFNHFYSQLVPAVTELVIAELMYLQWM 180
           PSLLL+GRIVYIGMP                          LVPAVTELVIA+LMYLQWM
Sbjct: 121 PSLLLHGRIVYIGMP--------------------------LVPAVTELVIAQLMYLQWM 180

Query: 181 DPKEPIYLYINSTGTTRDDGET--------------------IHTVAVGAAIGQACLLLA 240
           DPKEPIY+YINS+GTTRDDGET                    IHT+ VGAAIGQACLLLA
Sbjct: 181 DPKEPIYIYINSSGTTRDDGETVAMETEGFAIYDAMMQLKNEIHTLTVGAAIGQACLLLA 240

Query: 241 AGSQGKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHTGNSLE 300
           AG++G+RFMMPH+KAMIQQPRVPSSGLMPASD+LIRAKEVITNRDTLV+LLAKHTGNSLE
Sbjct: 241 AGTKGRRFMMPHSKAMIQQPRVPSSGLMPASDILIRAKEVITNRDTLVELLAKHTGNSLE 300

Query: 301 TVSNVMRRPFYMDSTRAKEFGVIDKILWRGQEKIMADVAAPEEWDKGAGIKVADE 336
           TV+N M+RPFYMD+ +AK+FGVIDKILWRGQEKIMA+VA+PE+WDKGAGIK  DE
Sbjct: 301 TVANAMKRPFYMDAIKAKQFGVIDKILWRGQEKIMAEVASPEDWDKGAGIKAVDE 329

BLAST of MS007299 vs. TAIR 10
Match: AT1G09130.3 (ATP-dependent caseinolytic (Clp) protease/crotonase family protein )

HSP 1 Score: 428.7 bits (1101), Expect = 4.3e-120
Identity = 233/355 (65.63%), Postives = 265/355 (74.65%), Query Frame = 0

Query: 9   MAISLQKPMNMASQSSSS-----SLARGTNFRTVCCT-----NATSKAKIPLPPINPKDP 68
           MA  LQ  MN     SSS      L+  ++ R    T      A + AKIP+PPINPKDP
Sbjct: 1   MASCLQASMNSLLPRSSSFSPHPPLSSNSSGRRNLKTFRYAFRAKASAKIPMPPINPKDP 60

Query: 69  FLSKLASVASTSPETLLNRPSNSDSPPYLDLFDAPQLMAAPAQVERSVSYNEHRPRRPPP 128
           FLS LAS+A+ SPE LLNRP N+D PPYLD+FD+PQLM++PAQVERSV+YNEHRPR PPP
Sbjct: 61  FLSTLASIAANSPEKLLNRPVNADVPPYLDIFDSPQLMSSPAQVERSVAYNEHRPRTPPP 120

Query: 129 DLPSLLLNGRIVYIGMPVKNFSFLCNAHCYFPDNSSFNHFYSQLVPAVTELVIAELMYLQ 188
           DLPS+LL+GRIVYIGMP                          LVPAVTELV+AELMYLQ
Sbjct: 121 DLPSMLLDGRIVYIGMP--------------------------LVPAVTELVVAELMYLQ 180

Query: 189 WMDPKEPIYLYINSTGTTRDDGET--------------------IHTVAVGAAIGQACLL 248
           W+DPKEPIY+YINSTGTTRDDGET                    +HTV VGAAIGQACLL
Sbjct: 181 WLDPKEPIYIYINSTGTTRDDGETVGMESEGFAIYDSLMQLKNEVHTVCVGAAIGQACLL 240

Query: 249 LAAGSQGKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHTGNS 308
           L+AG++GKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRD LV+LL+KHTGNS
Sbjct: 241 LSAGTKGKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDILVELLSKHTGNS 300

Query: 309 LETVSNVMRRPFYMDSTRAKEFGVIDKILWRGQEKIMADVAAPEEWDKGAGIKVA 334
           +ETV+NVMRRP+YMD+ +AKEFGVID+ILWRGQEKI+ADV   EE+DK AGIK A
Sbjct: 301 VETVANVMRRPYYMDAPKAKEFGVIDRILWRGQEKIIADVVPSEEFDKNAGIKSA 329

BLAST of MS007299 vs. TAIR 10
Match: AT1G09130.1 (ATP-dependent caseinolytic (Clp) protease/crotonase family protein )

HSP 1 Score: 427.9 bits (1099), Expect = 7.3e-120
Identity = 232/353 (65.72%), Postives = 264/353 (74.79%), Query Frame = 0

Query: 9   MAISLQKPMNMASQSSSS-----SLARGTNFRTVCCT-----NATSKAKIPLPPINPKDP 68
           MA  LQ  MN     SSS      L+  ++ R    T      A + AKIP+PPINPKDP
Sbjct: 1   MASCLQASMNSLLPRSSSFSPHPPLSSNSSGRRNLKTFRYAFRAKASAKIPMPPINPKDP 60

Query: 69  FLSKLASVASTSPETLLNRPSNSDSPPYLDLFDAPQLMAAPAQVERSVSYNEHRPRRPPP 128
           FLS LAS+A+ SPE LLNRP N+D PPYLD+FD+PQLM++PAQVERSV+YNEHRPR PPP
Sbjct: 61  FLSTLASIAANSPEKLLNRPVNADVPPYLDIFDSPQLMSSPAQVERSVAYNEHRPRTPPP 120

Query: 129 DLPSLLLNGRIVYIGMPVKNFSFLCNAHCYFPDNSSFNHFYSQLVPAVTELVIAELMYLQ 188
           DLPS+LL+GRIVYIGMP                          LVPAVTELV+AELMYLQ
Sbjct: 121 DLPSMLLDGRIVYIGMP--------------------------LVPAVTELVVAELMYLQ 180

Query: 189 WMDPKEPIYLYINSTGTTRDDGET--------------------IHTVAVGAAIGQACLL 248
           W+DPKEPIY+YINSTGTTRDDGET                    +HTV VGAAIGQACLL
Sbjct: 181 WLDPKEPIYIYINSTGTTRDDGETVGMESEGFAIYDSLMQLKNEVHTVCVGAAIGQACLL 240

Query: 249 LAAGSQGKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHTGNS 308
           L+AG++GKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRD LV+LL+KHTGNS
Sbjct: 241 LSAGTKGKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDILVELLSKHTGNS 300

Query: 309 LETVSNVMRRPFYMDSTRAKEFGVIDKILWRGQEKIMADVAAPEEWDKGAGIK 332
           +ETV+NVMRRP+YMD+ +AKEFGVID+ILWRGQEKI+ADV   EE+DK AGIK
Sbjct: 301 VETVANVMRRPYYMDAPKAKEFGVIDRILWRGQEKIIADVVPSEEFDKNAGIK 327

BLAST of MS007299 vs. TAIR 10
Match: AT1G09130.2 (ATP-dependent caseinolytic (Clp) protease/crotonase family protein )

HSP 1 Score: 427.9 bits (1099), Expect = 7.3e-120
Identity = 232/353 (65.72%), Postives = 264/353 (74.79%), Query Frame = 0

Query: 9   MAISLQKPMNMASQSSSS-----SLARGTNFRTVCCT-----NATSKAKIPLPPINPKDP 68
           MA  LQ  MN     SSS      L+  ++ R    T      A + AKIP+PPINPKDP
Sbjct: 1   MASCLQASMNSLLPRSSSFSPHPPLSSNSSGRRNLKTFRYAFRAKASAKIPMPPINPKDP 60

Query: 69  FLSKLASVASTSPETLLNRPSNSDSPPYLDLFDAPQLMAAPAQVERSVSYNEHRPRRPPP 128
           FLS LAS+A+ SPE LLNRP N+D PPYLD+FD+PQLM++PAQVERSV+YNEHRPR PPP
Sbjct: 61  FLSTLASIAANSPEKLLNRPVNADVPPYLDIFDSPQLMSSPAQVERSVAYNEHRPRTPPP 120

Query: 129 DLPSLLLNGRIVYIGMPVKNFSFLCNAHCYFPDNSSFNHFYSQLVPAVTELVIAELMYLQ 188
           DLPS+LL+GRIVYIGMP                          LVPAVTELV+AELMYLQ
Sbjct: 121 DLPSMLLDGRIVYIGMP--------------------------LVPAVTELVVAELMYLQ 180

Query: 189 WMDPKEPIYLYINSTGTTRDDGET--------------------IHTVAVGAAIGQACLL 248
           W+DPKEPIY+YINSTGTTRDDGET                    +HTV VGAAIGQACLL
Sbjct: 181 WLDPKEPIYIYINSTGTTRDDGETVGMESEGFAIYDSLMQLKNEVHTVCVGAAIGQACLL 240

Query: 249 LAAGSQGKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHTGNS 308
           L+AG++GKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRD LV+LL+KHTGNS
Sbjct: 241 LSAGTKGKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDILVELLSKHTGNS 300

Query: 309 LETVSNVMRRPFYMDSTRAKEFGVIDKILWRGQEKIMADVAAPEEWDKGAGIK 332
           +ETV+NVMRRP+YMD+ +AKEFGVID+ILWRGQEKI+ADV   EE+DK AGIK
Sbjct: 301 VETVANVMRRPYYMDAPKAKEFGVIDRILWRGQEKIIADVVPSEEFDKNAGIK 327

BLAST of MS007299 vs. TAIR 10
Match: AT4G17040.1 (CLP protease R subunit 4 )

HSP 1 Score: 134.8 bits (338), Expect = 1.3e-31
Identity = 85/213 (39.91%), Postives = 115/213 (53.99%), Query Frame = 0

Query: 115 RPPPDLPSLLLNGRIVYIGMPVKNFSFLCNAHCYFPDNSSFNHFYSQLVPAVTELVIAEL 174
           +PPPDL S L   RIVY+GM                           LVP+VTEL++AE 
Sbjct: 101 QPPPDLASYLFKNRIVYLGM--------------------------SLVPSVTELILAEF 160

Query: 175 MYLQWMDPKEPIYLYINSTGTTRD------DGET-------------IHTVAVGAAIGQA 234
           +YLQ+ D ++PIYLYINSTGTT++      D E              I T+ VG A G+A
Sbjct: 161 LYLQYEDEEKPIYLYINSTGTTKNGEKLGYDTEAFAIYDVMGYVKPPIFTLCVGNAWGEA 220

Query: 235 CLLLAAGSQGKRFMMPHAKAMIQQPRVPSSGLMPASDVLIRAKEVITNRDTLVKLLAKHT 294
            LLL AG++G R  +P +  MI+QP     G   A+DV I  KE+   +  +VKL +KH 
Sbjct: 221 ALLLTAGAKGNRSALPSSTIMIKQPIARFQG--QATDVEIARKEIKHIKTEMVKLYSKHI 280

Query: 295 GNSLETVSNVMRRPFYMDSTRAKEFGVIDKILW 309
           G S E +   M+RP Y   T A E+G+IDK+++
Sbjct: 281 GKSPEQIEADMKRPKYFSPTEAVEYGIIDKVVY 285

BLAST of MS007299 vs. TAIR 10
Match: AT1G49970.1 (CLP protease proteolytic subunit 1 )

HSP 1 Score: 134.4 bits (337), Expect = 1.7e-31
Identity = 82/216 (37.96%), Postives = 118/216 (54.63%), Query Frame = 0

Query: 112 RPRRPPPDLPSLLLNGRIVYIGMPVKNFSFLCNAHCYFPDNSSFNHFYSQLVPAVTELVI 171
           RPR  PPDLPSLLL+ RI Y+GMP+                          VPAVTEL++
Sbjct: 164 RPRTAPPDLPSLLLDARICYLGMPI--------------------------VPAVTELLV 223

Query: 172 AELMYLQWMDPKEPIYLYINSTGTTRDDGET--------------------IHTVAVGAA 231
           A+ M+L + +P +PIYLYINS GT  +  ET                    ++T+  G A
Sbjct: 224 AQFMWLDYDNPTKPIYLYINSPGTQNEKMETVGSETEAYAIADTISYCKSDVYTINCGMA 283

Query: 232 IGQACLLLAAGSQGKRFMMPHAKAMIQQPRV-PSSGLMPASDVLIRAKEVITNRDTLVKL 291
            GQA +LL+ G +G R + PH+   +  P+V  SSG   A D+ I+AKE+  N +  ++L
Sbjct: 284 FGQAAMLLSLGKKGYRAVQPHSSTKLYLPKVNRSSG--AAIDMWIKAKELDANTEYYIEL 343

Query: 292 LAKHTGNSLETVSNVMRRPFYMDSTRAKEFGVIDKI 307
           LAK TG S E ++  ++RP Y+ +  A ++G+ DKI
Sbjct: 344 LAKGTGKSKEQINEDIKRPKYLQAQAAIDYGIADKI 351

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150809.11.6e-16186.80ATP-dependent Clp protease proteolytic subunit-related protein 3, chloroplastic ... [more]
XP_038890491.16.1e-15381.46ATP-dependent Clp protease proteolytic subunit-related protein 3, chloroplastic ... [more]
XP_008454159.12.8e-14577.59PREDICTED: ATP-dependent Clp protease proteolytic subunit-related protein 3, chl... [more]
XP_023545293.12.2e-14276.34ATP-dependent Clp protease proteolytic subunit-related protein 3, chloroplastic ... [more]
XP_022946246.13.7e-14276.34ATP-dependent Clp protease proteolytic subunit-related protein 3, chloroplastic ... [more]
Match NameE-valueIdentityDescription
Q8L7701.0e-11865.72ATP-dependent Clp protease proteolytic subunit-related protein 3, chloroplastic ... [more]
P744662.1e-3437.50Putative ATP-dependent Clp protease proteolytic subunit-like OS=Synechocystis sp... [more]
Q9L4P41.3e-3336.21Putative ATP-dependent Clp protease proteolytic subunit-like OS=Synechococcus el... [more]
Q8LB101.8e-3039.91ATP-dependent Clp protease proteolytic subunit-related protein 4, chloroplastic ... [more]
Q9XJ352.3e-3037.96ATP-dependent Clp protease proteolytic subunit-related protein 1, chloroplastic ... [more]
Match NameE-valueIdentityDescription
A0A6J1D9I57.8e-16286.80ATP-dependent Clp protease proteolytic subunit OS=Momordica charantia OX=3673 GN... [more]
A0A1S3BXZ31.3e-14577.59ATP-dependent Clp protease proteolytic subunit OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A5A7U3K51.3e-14577.59ATP-dependent Clp protease proteolytic subunit OS=Cucumis melo var. makuwa OX=11... [more]
A0A6J1G3791.8e-14276.34ATP-dependent Clp protease proteolytic subunit OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1KFX91.9e-13974.65ATP-dependent Clp protease proteolytic subunit OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
AT1G09130.34.3e-12065.63ATP-dependent caseinolytic (Clp) protease/crotonase family protein [more]
AT1G09130.17.3e-12065.72ATP-dependent caseinolytic (Clp) protease/crotonase family protein [more]
AT1G09130.27.3e-12065.72ATP-dependent caseinolytic (Clp) protease/crotonase family protein [more]
AT4G17040.11.3e-3139.91CLP protease R subunit 4 [more]
AT1G49970.11.7e-3137.96CLP protease proteolytic subunit 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001907ATP-dependent Clp protease proteolytic subunitPRINTSPR00127CLPPROTEASEPcoord: 205..222
score: 42.08
coord: 119..134
score: 36.41
coord: 226..245
score: 37.38
coord: 285..304
score: 34.75
IPR001907ATP-dependent Clp protease proteolytic subunitCDDcd07017S14_ClpP_2coord: 128..306
e-value: 6.09606E-61
score: 190.73
IPR023562Clp protease proteolytic subunit /Translocation-enhancing protein TepAPFAMPF00574CLP_proteasecoord: 163..307
e-value: 2.0E-32
score: 112.5
IPR023562Clp protease proteolytic subunit /Translocation-enhancing protein TepAPANTHERPTHR10381ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNITcoord: 202..336
coord: 28..197
NoneNo IPR availableGENE3D3.90.226.10coord: 93..311
e-value: 3.3E-41
score: 143.1
NoneNo IPR availablePANTHERPTHR10381:SF6ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT-RELATED PROTEIN 3, CHLOROPLASTICcoord: 202..336
coord: 28..197
IPR029045ClpP/crotonase-like domain superfamilySUPERFAMILY52096ClpP/crotonasecoord: 114..307

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS007299.1MS007299.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004176 ATP-dependent peptidase activity
molecular_function GO:0004252 serine-type endopeptidase activity