MS007242 (gene) Bitter gourd (TR) v1

Overview
NameMS007242
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionintermediate cleaving peptidase 55, mitochondrial isoform X1
Locationscaffold25: 1684090 .. 1689820 (-)
RNA-Seq ExpressionMS007242
SyntenyMS007242
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAGAAGGGGAAATTACTCCTGGCATAACTGCTGAGGAATACATTACAAGAAGAAAAAGGTTGCTAGAGCTTCTACCAGAGAAGAGTGTGGCCATTATTGCAGCTGCCCCAGTAAAAATGATGACTGATGTGGTGCCTTACACATTTCGACAAGATGCTGATTACTTATACGTCACTGGTTGCCTACAACAGGGTGGTCTGGCAGTTTTGAGTCGCGAATTTGGATTCTGCATGTTCATGCCTGAAGCAAAGCCTCATGTATGGCCAATCTTATTCATCCTACCAAACTTTACTTTGTGCCCTGGCAATTTAGGTCCTTTAACAGCAATCACTTTTCAAGTGGTTGAATATAAAAGTTAAGGAACATGGTCAAGTTTATAACCAAATATTAAATTTTGCACCTCTCCGTTAATGTTATTTTTTTTTTTGATAAGAAACTTAGAGAAGTGTATTAATCACAAAGGGAGAACAGCCTAAGGGCAAGGGGTAGAGGAAACCCCTCCCCAACACAAAACTACTACATGAACGCCTTCCAATTGTGAAGGATCGAAAGAAGGGAGTAGTTGCAAAAGAATTTACTAAAACTATGACACCACCTAGAAGCAGTGAGCTGAATATTACTCCACAAAACGTGAAAATCTAAAGAAGAATCATCAAAGTAACATCCGATAATGTTATATGTAACTATATTCTTCTGTGGCCATATATATTTCTCTTCATTTTTCTACTATTCATTTTCACCGTAACACCATCATCGTCTTCTCCTCATTGGCTGGCCTTCAGGAGTATCTTTATCAACAAGGAGGCAATGAATAAACCATGAGGGAAAAAGATGGAAAAGAGAGATCACAGACTGTCCAACCAAATATCCTCTTAAAATTTATTTGCTCCATTACTTAAAAGCAATTTTGGCACATGGAAGGAGGGATCTTGTGGAATGAATTTCCATGGACTGTGATAAGAGACCCTTTGAACCAAGATGGAATTCAATCATTTTGAAGCAAAACATATTTATTTTTAGGTTAAATTACATGTTCAGTCCTTAAATTTTTAGTGTTCGTTGCGTCTAATAGATCCCTGAACTTTTAAAAAGTATATAATGGATCTCTAAATTTTCATTTTGTGTTTAATAGGTGAGTCCTTAAATTTGAAAAGTGTTTTATAGGTCATTGAACTTTCAATTTTGTGACATTCCAAATTTGGCGTATTTATGGTCTTTTACTGGTGTAGCACTATGTTGGCTAATTTCACGAGAATGCAACAAGTTGGTTATGGGAATGAAAAGTGTTTTCAATCTTTTTGAGGGAAGTTGGTTTGAAGATCTTTCATCATGAATTTTTCGATCAGTTGGTTAGCTCATGGATTGAGGCACCATGATTCATCTATTTCCTGAAATTTCTTTATTAATTTTTGCTATGTTATTTGCTGACGTGGAGGCTATCTTATCCTCAGACCTTTCCTTGTATGCATCTTTCATTTATTAGTATATTTTATTATTTCTGATTAAAGAAAATGCATTTAGTTATTTACCATTCATGTGGATAATCTTGTAAAGCGAGTAATGCCAGTCGCATACATCCTGAGTTTGATTTCATTATATTTTATCAGGATGTTCTATGGCAAGGGAAAATTGCAGGAGTTGATGCAGCATTGGATTTTTTCAAGGCTGATGAAGCATATCCCATGAAGAAGTTGCGTGAGGTGAGTTCATTTGAAGAATATATTGGCAATTATGGAATTATTGTTAACTTGAATCAAGACCATGAATTTATTGCTGATCTATAAGTTACAGCTTATCCAATTCAACTAACCATGTATCGCTGATTATATTTTCAATCTGTGGCAAAAAAAATTCCATCTTTCAATATGATTCTGGTGTAACCAATACTGACCTCTGTTTGCTGCGTCAAGGCTGACCAGCATCATGAGTTTTGTTTGTAAGATTCTGCATCCAATGCTGGTCTGAATGTGTAACTGAATGGAGCATTTTAATTTTTTCACTGTCATTAAGTCTTCTATAATACTTTGTACTAGTACCACTTGTGTTTCGCTTTTTACCCTCTATATTTTATTACAGATCCTTCCAGATATTATTAGGAGGTCCACCAAGTTGTTCCATAATTCGACTACTGCCACTCCAGCTTATGTGGGTTTAGAGGCCTTCCAAAAAGCGGCCGAGAATAACCGTGTGCAAGATATTTCACTTTTAACTCATGAACTGCGGTTAATAAAATCAACAGCAGAGATGAAGTTAATGAGGGATTCTGCTTCGATTGCTTGCCAGGTACCTCCAAGCCTTTGGTTGGTTATCCAACTGGCTCCATGATCGGTCATGAATAGTTTTCCTTTTGAATTCAGCTAATTGTTAAGAGATAATCTAACAAGTGGCAGAAATGATTGGGGATGTGGAATAACATTGACATTGCCATTTTCCCCACCTTAAAAGCATGCCTATTTCTGCACCTGCACCATGTTGTCAAATCCTTTCTAAGTTCACTTTTATACAAATGGTGCTTTGATTCAGGCTCTACTGCAGACGATGATGCATTCAAAAACATATCCTTATGAGAGCAAGTTATCAGCAAAAGTAGAATATGAGTGCAGAATGAGAGGTTCTCAAAGAATGGCGTGAGTAGTTTCTAGTTTATTTCTTCGTATTTGGATCTAAAGAAGTACCCCCATTTTGATGTTTGCTTGGTATTCTTAATATTAGTACGATGGCTAGTGCAAATTGTGAACGGGGTCTAGCTTTAATATGTGACTATAAATTATGCAAGATTTTCAACTGATTTTCGATCTCCATTCTCTGTAACAGATTTAATCCTGTGACTGGTGGTGGGTCTAATGGAAGTGTTATACATTATTCTCGAAATGATCAGAAAGTAAGTGCATCTATCACATGGAGCCTAAGCTTCACCATTAAGAAAGATGGAAAGAAAATTATGAATATTCATCTGTATGTACATGAAAGAAAAATGATCGCAGGTCAGAGAAGGGGATTTGGTCTTAATGGATATTGGATGTGAATTGCACGGTTATGTCAGTGATCTTACTCGTACCTGGCCGCCTTGTGGGAACTTTTCTGCTGCTCAGGTATTTAATCTTTATCCCATAGTCTTATTTATTTACAGTCATTATGAGGATTTTGAGCCTCTTCTTGTTTGTGTGTGTTGTGACCTCAAAATCCTTTCTCCATTCGTTTTTGTGAACTTGAAAATATTTCCTTTGAATAGATCATTTTAACTACTTTTCACTACTCATTAAGCAATGATTATGTGAGTTCAGTTTATATATACATGCACAATCATCTCTATTGATTGTTCAATCTTGTTGCTTTTCTATTGGATCTTACCTTTATTTTGTATTACTTTCCCTCTTGTTTTGTTACCAACTTGCTTAGTTCAATGTTATCTCAGGAAGAGCTTTATGATCTTATACTGCAAACAAACAAGGAATGCATAAAGCTTTGCAAACCTGGCGCTACTATACGGGACATTCATAACTACTCGGTACATTTTGTGTATTTATTTGTTGTTCAATTGTTCAAAGTTTAATTGATATATAGATCATGGGAATAAATCCCATCAGTCAATTAATAAAGCTTTTTCTAGTTCTATTGTCGAGGTTCTTGACCTTTTCTTCCAAGGTTGCAACATGCAAGCATCTCTTTGGTCTTTCCTTGGATCGAGTGAAATTGAAAGAAGTTTTATGGTGCAAAAAGATCTTGAGAGGAAGTTTAGATAGTATCTTGTTTCTCTGTGGTCTATTCTTTTTCAAGTTTTCTTTGTAACTATTTGATGATTTCTTCCATTTTTGGCCAGTTCCTCACTTAACTAAGCATCTGGCCACTTGATCTCTGAAAGAAATATTATAAACCTTTTCATATTCTACATTTTTGCAGGTGCAAATGCTACGGAAAGGCTTCAAAGAGCTTGGAATTTTGCAAAATTGGAAAAGCGAGTCCTACCACCAGCTGAATCCCACTTCTATAGGTTATTTTCTACTTAAAATTAATCTGTTGTTTATTGACAAAAGCGCGGTACTTTTGATGTTATGCCACCTTTTTCTCTTCAACAATACATAATTTTCCAATTGGAGTGATTTCCTAAGTTGGCTCCCTGATTAGCCATCTTTCTTTCTGTTTTTTGATTCCTCTTCCCAGGTCATTATCTTGGAATGGATGTCCATGATTGCTCCATGGTTGGTTACGACCGGCCATTGAAGCCAGGTGTTGTAAGTTCTGTTCCCGTTCATATATATGGACACCACCCTTCCCTCCCCGTCTTCCTGTCCCTATACATGATTATGTTCATCCCATTTATGGACGATACGAGCTTAAACTTCAATGATACTGTCTTCTGCATGCTTTTTATGTTGTAGGAATACTAGCATTTGTCACAATGTCATTTTTTTCTAGTAAGATAGAGTGTTTACATTATAATTGGAGACAAGTTGAATATCAGTACACTGGTTTTGTATTTATTTTAAATTCAAATTGAAGAGCTTCTTGATTCTTTGCAGGTGATAACAATTGAACCTGGAGTTTACATCCCATCCTTATTTGAAGGTCCCGAGAGGTAAACTATACACGTTAAACTTACCTATGAAAAATGTGTAAAAATGATTTAAGTTAGAAGCTCATCAAATGAAAAAAGATGAGTTAATCTTTCAACATAGTTTCTTGTTATTTCCATTGGCCAAGATAGGAAGCCGGGTCCAATCGTTGACGAACATCTCCATTTCATAAGTTTTTGAAGAGATGAATTTTTCATTGTATGAGCTACAGACTTCCATAATAAGTAACACTAAAACACACCAATAACATTATAATGGTGTGAAGGGTGTTCGAGAGTATAGGAAGAGACATAATCGACCTTGCCTTTTACATAACTTTCATTATTTGGGATCCTATTTATCTCTTTAGACTTTTCCCATCCTTTTGATATAGAAATTGAATGCTTGACTCCAACCGGCCCAGCAAAAGAAAATACTGAAAATTACAATAAAGCACTCTGTCAAGTAAAAATGTAAGAGGAAAGAAACTTTTAATTATTTGGATTGATCTCATCAACAACTCTGCCCACACAACCTTCTGACTAAAACTTGATCCTCAATGAGAGATGTCTGTTTTCCAATTGCATATTTCCAAATTTGTTTATATATTGGAACAAAAGTTTCTCATTAGACAACTTACTCTGCAGGTTTGCTGGTATCGGGATACGGATTGAGGATGAAGTCCTTATTACTGAATCGGGTCACGAGGTACCTAATTTGAGTCTTAAGTTCTTTTTTGGATGGTTGTGACAGTTGAGTAAAGAGGAATATGAGAAAAGTTTACAGTCGAGCAATATAATTATGGTCCAAGACTAAAACTTTTATTATCCACAATATGTCTAAGGTAATCATAATTTCATTTCCTATGAAGGGGCGACTCCATTGGCAAGGCATGGGGCCTTGTGGTATTATGTACCATGAGGTCTTGTGTTCAAGCCTTTAGCTGAGCTTAATACAAAAAATTCCATATTAAACATACTACAATTTTGTTAAAAAACACTTTCCCAAATCCTGGAAGCCTTTAATATTTAGGAGTGAATTAATCAATTATGCTTTCTTGTATTAGGTTCTTACAGGTTCGATCCCAAAAGAAATTAAACAGATAGAGTCATTGCTGAACAACTTCAGCTTTGGATCCGTGAACAGTTTTAACTGTATGAAAGCCTCAACTTTT

mRNA sequence

ATGAAAGAAGGGGAAATTACTCCTGGCATAACTGCTGAGGAATACATTACAAGAAGAAAAAGGTTGCTAGAGCTTCTACCAGAGAAGAGTGTGGCCATTATTGCAGCTGCCCCAGTAAAAATGATGACTGATGTGGTGCCTTACACATTTCGACAAGATGCTGATTACTTATACGTCACTGGTTGCCTACAACAGGGTGGTCTGGCAGTTTTGAGTCGCGAATTTGGATTCTGCATGTTCATGCCTGAAGCAAAGCCTCATGATGTTCTATGGCAAGGGAAAATTGCAGGAGTTGATGCAGCATTGGATTTTTTCAAGGCTGATGAAGCATATCCCATGAAGAAGTTGCGTGAGATCCTTCCAGATATTATTAGGAGGTCCACCAAGTTGTTCCATAATTCGACTACTGCCACTCCAGCTTATGTGGGTTTAGAGGCCTTCCAAAAAGCGGCCGAGAATAACCGTGTGCAAGATATTTCACTTTTAACTCATGAACTGCGGTTAATAAAATCAACAGCAGAGATGAAGTTAATGAGGGATTCTGCTTCGATTGCTTGCCAGGCTCTACTGCAGACGATGATGCATTCAAAAACATATCCTTATGAGAGCAAGTTATCAGCAAAAGTAGAATATGAGTGCAGAATGAGAGGTTCTCAAAGAATGGCATTTAATCCTGTGACTGGTGGTGGGTCTAATGGAAGTGTTATACATTATTCTCGAAATGATCAGAAAGTCAGAGAAGGGGATTTGGTCTTAATGGATATTGGATGTGAATTGCACGGTTATGTCAGTGATCTTACTCGTACCTGGCCGCCTTGTGGGAACTTTTCTGCTGCTCAGGAAGAGCTTTATGATCTTATACTGCAAACAAACAAGGAATGCATAAAGCTTTGCAAACCTGGCGCTACTATACGGGACATTCATAACTACTCGGTGCAAATGCTACGGAAAGGCTTCAAAGAGCTTGGAATTTTGCAAAATTGGAAAAGCGAGTCCTACCACCAGCTGAATCCCACTTCTATAGGTCATTATCTTGGAATGGATGTCCATGATTGCTCCATGGTTGGTTACGACCGGCCATTGAAGCCAGGTGTTGTGATAACAATTGAACCTGGAGTTTACATCCCATCCTTATTTGAAGGTCCCGAGAGGTTTGCTGGTATCGGGATACGGATTGAGGATGAAGTCCTTATTACTGAATCGGGTCACGAGGTTCTTACAGGTTCGATCCCAAAAGAAATTAAACAGATAGAGTCATTGCTGAACAACTTCAGCTTTGGATCCGTGAACAGTTTTAACTGTATGAAAGCCTCAACTTTT

Coding sequence (CDS)

ATGAAAGAAGGGGAAATTACTCCTGGCATAACTGCTGAGGAATACATTACAAGAAGAAAAAGGTTGCTAGAGCTTCTACCAGAGAAGAGTGTGGCCATTATTGCAGCTGCCCCAGTAAAAATGATGACTGATGTGGTGCCTTACACATTTCGACAAGATGCTGATTACTTATACGTCACTGGTTGCCTACAACAGGGTGGTCTGGCAGTTTTGAGTCGCGAATTTGGATTCTGCATGTTCATGCCTGAAGCAAAGCCTCATGATGTTCTATGGCAAGGGAAAATTGCAGGAGTTGATGCAGCATTGGATTTTTTCAAGGCTGATGAAGCATATCCCATGAAGAAGTTGCGTGAGATCCTTCCAGATATTATTAGGAGGTCCACCAAGTTGTTCCATAATTCGACTACTGCCACTCCAGCTTATGTGGGTTTAGAGGCCTTCCAAAAAGCGGCCGAGAATAACCGTGTGCAAGATATTTCACTTTTAACTCATGAACTGCGGTTAATAAAATCAACAGCAGAGATGAAGTTAATGAGGGATTCTGCTTCGATTGCTTGCCAGGCTCTACTGCAGACGATGATGCATTCAAAAACATATCCTTATGAGAGCAAGTTATCAGCAAAAGTAGAATATGAGTGCAGAATGAGAGGTTCTCAAAGAATGGCATTTAATCCTGTGACTGGTGGTGGGTCTAATGGAAGTGTTATACATTATTCTCGAAATGATCAGAAAGTCAGAGAAGGGGATTTGGTCTTAATGGATATTGGATGTGAATTGCACGGTTATGTCAGTGATCTTACTCGTACCTGGCCGCCTTGTGGGAACTTTTCTGCTGCTCAGGAAGAGCTTTATGATCTTATACTGCAAACAAACAAGGAATGCATAAAGCTTTGCAAACCTGGCGCTACTATACGGGACATTCATAACTACTCGGTGCAAATGCTACGGAAAGGCTTCAAAGAGCTTGGAATTTTGCAAAATTGGAAAAGCGAGTCCTACCACCAGCTGAATCCCACTTCTATAGGTCATTATCTTGGAATGGATGTCCATGATTGCTCCATGGTTGGTTACGACCGGCCATTGAAGCCAGGTGTTGTGATAACAATTGAACCTGGAGTTTACATCCCATCCTTATTTGAAGGTCCCGAGAGGTTTGCTGGTATCGGGATACGGATTGAGGATGAAGTCCTTATTACTGAATCGGGTCACGAGGTTCTTACAGGTTCGATCCCAAAAGAAATTAAACAGATAGAGTCATTGCTGAACAACTTCAGCTTTGGATCCGTGAACAGTTTTAACTGTATGAAAGCCTCAACTTTT

Protein sequence

MKEGEITPGITAEEYITRRKRLLELLPEKSVAIIAAAPVKMMTDVVPYTFRQDADYLYVTGCLQQGGLAVLSREFGFCMFMPEAKPHDVLWQGKIAGVDAALDFFKADEAYPMKKLREILPDIIRRSTKLFHNSTTATPAYVGLEAFQKAAENNRVQDISLLTHELRLIKSTAEMKLMRDSASIACQALLQTMMHSKTYPYESKLSAKVEYECRMRGSQRMAFNPVTGGGSNGSVIHYSRNDQKVREGDLVLMDIGCELHGYVSDLTRTWPPCGNFSAAQEELYDLILQTNKECIKLCKPGATIRDIHNYSVQMLRKGFKELGILQNWKSESYHQLNPTSIGHYLGMDVHDCSMVGYDRPLKPGVVITIEPGVYIPSLFEGPERFAGIGIRIEDEVLITESGHEVLTGSIPKEIKQIESLLNNFSFGSVNSFNCMKASTF
Homology
BLAST of MS007242 vs. NCBI nr
Match: XP_022156404.1 (probable Xaa-Pro aminopeptidase 3 isoform X1 [Momordica charantia])

HSP 1 Score: 892.5 bits (2305), Expect = 1.5e-255
Identity = 436/440 (99.09%), Postives = 439/440 (99.77%), Query Frame = 0

Query: 1   MKEGEITPGITAEEYITRRKRLLELLPEKSVAIIAAAPVKMMTDVVPYTFRQDADYLYVT 60
           MKEGEITPGITAEEYITRRKRLLELLPEKSVAIIAAAPVKMMTDVVPYTFRQDADYLY+T
Sbjct: 43  MKEGEITPGITAEEYITRRKRLLELLPEKSVAIIAAAPVKMMTDVVPYTFRQDADYLYIT 102

Query: 61  GCLQQGGLAVLSREFGFCMFMPEAKPHDVLWQGKIAGVDAALDFFKADEAYPMKKLREIL 120
           GCLQQGGLAVLSREFGFCMFMPEAKPHDVLWQGKIAGVDAALDFFKADEAYPMKKLREIL
Sbjct: 103 GCLQQGGLAVLSREFGFCMFMPEAKPHDVLWQGKIAGVDAALDFFKADEAYPMKKLREIL 162

Query: 121 PDIIRRSTKLFHNSTTATPAYVGLEAFQKAAENNRVQDISLLTHELRLIKSTAEMKLMRD 180
           PDIIRRSTKLFHNSTTATPAYVGLEAFQKAAENNRVQDISLLTHELRLIKSTAEMKLMRD
Sbjct: 163 PDIIRRSTKLFHNSTTATPAYVGLEAFQKAAENNRVQDISLLTHELRLIKSTAEMKLMRD 222

Query: 181 SASIACQALLQTMMHSKTYPYESKLSAKVEYECRMRGSQRMAFNPVTGGGSNGSVIHYSR 240
           SASIACQALLQTMMHSKTYPYESKLSAKVEYECRMRG+QRMAFNPVTGGGSNGSVIHYSR
Sbjct: 223 SASIACQALLQTMMHSKTYPYESKLSAKVEYECRMRGAQRMAFNPVTGGGSNGSVIHYSR 282

Query: 241 NDQKVREGDLVLMDIGCELHGYVSDLTRTWPPCGNFSAAQEELYDLILQTNKECIKLCKP 300
           NDQKVREGDLVLMDIGCELHGYVSDLTRTWPPCGNFSAAQEELYDLILQTNKECIKLCKP
Sbjct: 283 NDQKVREGDLVLMDIGCELHGYVSDLTRTWPPCGNFSAAQEELYDLILQTNKECIKLCKP 342

Query: 301 GATIRDIHNYSVQMLRKGFKELGILQNWKSESYHQLNPTSIGHYLGMDVHDCSMVGYDRP 360
           GATIRDIHNYSVQMLRKGFKELGILQNWKSESYHQLNPTSIGHYLGMDVHDCSMVGYDRP
Sbjct: 343 GATIRDIHNYSVQMLRKGFKELGILQNWKSESYHQLNPTSIGHYLGMDVHDCSMVGYDRP 402

Query: 361 LKPGVVITIEPGVYIPSLFEGPERFAGIGIRIEDEVLITESGHEVLTGSIPKEIKQIESL 420
           LK GVVITIEPGVYIPSLFEGPERFAGIGIRIED+VLITESGHEVLTGSIPKEIKQIESL
Sbjct: 403 LKXGVVITIEPGVYIPSLFEGPERFAGIGIRIEDDVLITESGHEVLTGSIPKEIKQIESL 462

Query: 421 LNNFSFGSVNSFNCMKASTF 441
           LNNFSFGSVNSFNCMKASTF
Sbjct: 463 LNNFSFGSVNSFNCMKASTF 482

BLAST of MS007242 vs. NCBI nr
Match: XP_023537266.1 (intermediate cleaving peptidase 55, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo] >XP_023537267.1 intermediate cleaving peptidase 55, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo] >XP_023537268.1 intermediate cleaving peptidase 55, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo] >XP_023537270.1 intermediate cleaving peptidase 55, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 790.4 bits (2040), Expect = 7.9e-225
Identity = 381/440 (86.59%), Postives = 408/440 (92.73%), Query Frame = 0

Query: 1   MKEGEITPGITAEEYITRRKRLLELLPEKSVAIIAAAPVKMMTDVVPYTFRQDADYLYVT 60
           + EGEITPGITAEEYITRRKRLLELLP+  +AIIAAAPVKMMTDVVPYTFRQDADYLY+T
Sbjct: 43  LNEGEITPGITAEEYITRRKRLLELLPQNGLAIIAAAPVKMMTDVVPYTFRQDADYLYIT 102

Query: 61  GCLQQGGLAVLSREFGFCMFMPEAKPHDVLWQGKIAGVDAALDFFKADEAYPMKKLREIL 120
           GCLQ GGLAV+S EFG CMFMPE KP+DVLWQG+ AGVDAAL+FFKAD+AYPMKKLREIL
Sbjct: 103 GCLQPGGLAVMSHEFGLCMFMPETKPYDVLWQGQTAGVDAALEFFKADQAYPMKKLREIL 162

Query: 121 PDIIRRSTKLFHNSTTATPAYVGLEAFQKAAENNRVQDISLLTHELRLIKSTAEMKLMRD 180
           PDIIR+S+KLFHNS TATP Y+ LEAF+KAAENN V+DISL THELRLIKSTAE KLMRD
Sbjct: 163 PDIIRKSSKLFHNSNTATPTYLDLEAFRKAAENNCVKDISLFTHELRLIKSTAEQKLMRD 222

Query: 181 SASIACQALLQTMMHSKTYPYESKLSAKVEYECRMRGSQRMAFNPVTGGGSNGSVIHYSR 240
           SASIACQALLQTM+HSKTYP+ES LSAKVEYECRMRG+QRMAFNPV GGG NGSVIHYSR
Sbjct: 223 SASIACQALLQTMIHSKTYPHESMLSAKVEYECRMRGAQRMAFNPVVGGGCNGSVIHYSR 282

Query: 241 NDQKVREGDLVLMDIGCELHGYVSDLTRTWPPCGNFSAAQEELYDLILQTNKECIKLCKP 300
           NDQKVR+GD VLMD+GCELHGYVSDLTRTWPPCG FSAAQEELY+LILQTNKECIKLCKP
Sbjct: 283 NDQKVRDGDFVLMDVGCELHGYVSDLTRTWPPCGKFSAAQEELYELILQTNKECIKLCKP 342

Query: 301 GATIRDIHNYSVQMLRKGFKELGILQNWKSESYHQLNPTSIGHYLGMDVHDCSMVGYDRP 360
           GATI +IHNYSV+ LRKGFKELGILQNWKS+ YH LNPTSIGHYLGMDVHDC+ VGYDRP
Sbjct: 343 GATILEIHNYSVETLRKGFKELGILQNWKSDFYHHLNPTSIGHYLGMDVHDCARVGYDRP 402

Query: 361 LKPGVVITIEPGVYIPSLFEGPERFAGIGIRIEDEVLITESGHEVLTGSIPKEIKQIESL 420
           LKPGVVITIEPGVY+PS FEG ERFAGIGIRIEDEVLITESGHEVLTGSIPKEI QIESL
Sbjct: 403 LKPGVVITIEPGVYVPSSFEGSERFAGIGIRIEDEVLITESGHEVLTGSIPKEINQIESL 462

Query: 421 LNNFSFGS-VNSFNCMKAST 440
           LNNF FGS ++  N MKAST
Sbjct: 463 LNNFCFGSTMHGLNFMKAST 482

BLAST of MS007242 vs. NCBI nr
Match: XP_022937804.1 (intermediate cleaving peptidase 55, mitochondrial isoform X1 [Cucurbita moschata] >XP_022937805.1 intermediate cleaving peptidase 55, mitochondrial isoform X1 [Cucurbita moschata] >XP_022937806.1 intermediate cleaving peptidase 55, mitochondrial isoform X1 [Cucurbita moschata])

HSP 1 Score: 789.3 bits (2037), Expect = 1.8e-224
Identity = 380/440 (86.36%), Postives = 407/440 (92.50%), Query Frame = 0

Query: 1   MKEGEITPGITAEEYITRRKRLLELLPEKSVAIIAAAPVKMMTDVVPYTFRQDADYLYVT 60
           + EGEITPGITAEEYITRRKRLLELLP+  +AIIAAAPVKMMTDVVPYTFRQDADYLY+T
Sbjct: 43  LNEGEITPGITAEEYITRRKRLLELLPQNGLAIIAAAPVKMMTDVVPYTFRQDADYLYIT 102

Query: 61  GCLQQGGLAVLSREFGFCMFMPEAKPHDVLWQGKIAGVDAALDFFKADEAYPMKKLREIL 120
           GCLQ GGLAV+S EFG CMFMPE KP+DVLWQG+ AGVDAAL+FFK D+AYPMKKLREIL
Sbjct: 103 GCLQPGGLAVMSHEFGLCMFMPETKPYDVLWQGQTAGVDAALEFFKGDQAYPMKKLREIL 162

Query: 121 PDIIRRSTKLFHNSTTATPAYVGLEAFQKAAENNRVQDISLLTHELRLIKSTAEMKLMRD 180
           PDIIRRS+KLFHNS TATP Y+ LEAF+KAAENN V+DISL THELRLIKSTAE KLMRD
Sbjct: 163 PDIIRRSSKLFHNSNTATPTYLDLEAFRKAAENNCVKDISLFTHELRLIKSTAEQKLMRD 222

Query: 181 SASIACQALLQTMMHSKTYPYESKLSAKVEYECRMRGSQRMAFNPVTGGGSNGSVIHYSR 240
           SASIACQALLQTM+HSKTYP+ES LSAKVEYECRMRG+QRMAFNPV GGG NGSVIHYSR
Sbjct: 223 SASIACQALLQTMIHSKTYPHESMLSAKVEYECRMRGAQRMAFNPVVGGGCNGSVIHYSR 282

Query: 241 NDQKVREGDLVLMDIGCELHGYVSDLTRTWPPCGNFSAAQEELYDLILQTNKECIKLCKP 300
           NDQKVR+GD VLMD+GCELHGYVSDLTRTWPPCG FSAAQEELY+LILQTNKECIKLCKP
Sbjct: 283 NDQKVRDGDFVLMDVGCELHGYVSDLTRTWPPCGKFSAAQEELYELILQTNKECIKLCKP 342

Query: 301 GATIRDIHNYSVQMLRKGFKELGILQNWKSESYHQLNPTSIGHYLGMDVHDCSMVGYDRP 360
           GATI +IHNYSV+ LRKGFKELGILQNWKS+ YH LNPTSIGHYLGMDVHDC+ VGYDRP
Sbjct: 343 GATILEIHNYSVETLRKGFKELGILQNWKSDFYHHLNPTSIGHYLGMDVHDCARVGYDRP 402

Query: 361 LKPGVVITIEPGVYIPSLFEGPERFAGIGIRIEDEVLITESGHEVLTGSIPKEIKQIESL 420
           LKPGVVITIEPGVY+PS FEG ERFAGIGIRIEDEVLITESGHEVLTGSIPKEI QIES+
Sbjct: 403 LKPGVVITIEPGVYVPSSFEGSERFAGIGIRIEDEVLITESGHEVLTGSIPKEINQIESM 462

Query: 421 LNNFSFGS-VNSFNCMKAST 440
           LNNF FGS ++  N MKAST
Sbjct: 463 LNNFCFGSTMHGLNFMKAST 482

BLAST of MS007242 vs. NCBI nr
Match: XP_022965697.1 (intermediate cleaving peptidase 55, mitochondrial isoform X1 [Cucurbita maxima] >XP_022965698.1 intermediate cleaving peptidase 55, mitochondrial isoform X1 [Cucurbita maxima] >XP_022965699.1 intermediate cleaving peptidase 55, mitochondrial isoform X1 [Cucurbita maxima])

HSP 1 Score: 788.1 bits (2034), Expect = 3.9e-224
Identity = 381/440 (86.59%), Postives = 406/440 (92.27%), Query Frame = 0

Query: 1   MKEGEITPGITAEEYITRRKRLLELLPEKSVAIIAAAPVKMMTDVVPYTFRQDADYLYVT 60
           + EGEITPGITAEEYITRRKRLLELLP+  +AIIAAAPVKMMTDVVPYTFRQDADYLY+T
Sbjct: 43  LNEGEITPGITAEEYITRRKRLLELLPQNGLAIIAAAPVKMMTDVVPYTFRQDADYLYIT 102

Query: 61  GCLQQGGLAVLSREFGFCMFMPEAKPHDVLWQGKIAGVDAALDFFKADEAYPMKKLREIL 120
           GCLQ GGLAV+S EFG CMFMPE KP+DVLWQGKIAGVDAAL+FFKAD+AYPM KLREIL
Sbjct: 103 GCLQPGGLAVMSHEFGLCMFMPETKPYDVLWQGKIAGVDAALEFFKADQAYPMTKLREIL 162

Query: 121 PDIIRRSTKLFHNSTTATPAYVGLEAFQKAAENNRVQDISLLTHELRLIKSTAEMKLMRD 180
           PDIIRRS+KLFHNS TAT  Y+ LEAF+KAAENN V+DISL THELRLIKSTAE KLMRD
Sbjct: 163 PDIIRRSSKLFHNSNTATSTYLDLEAFRKAAENNCVKDISLFTHELRLIKSTAEQKLMRD 222

Query: 181 SASIACQALLQTMMHSKTYPYESKLSAKVEYECRMRGSQRMAFNPVTGGGSNGSVIHYSR 240
           SASIACQALLQTM+HSKTYP+ES LSAKVEYECRMRG+QRMAFNPV GGG NGSVIHYSR
Sbjct: 223 SASIACQALLQTMIHSKTYPHESMLSAKVEYECRMRGAQRMAFNPVVGGGCNGSVIHYSR 282

Query: 241 NDQKVREGDLVLMDIGCELHGYVSDLTRTWPPCGNFSAAQEELYDLILQTNKECIKLCKP 300
           NDQKVR+GD VLMD+GCELHGYVSDLTRTWPPCG FSA QEELY+LILQTNKECIKLCKP
Sbjct: 283 NDQKVRDGDFVLMDVGCELHGYVSDLTRTWPPCGKFSATQEELYELILQTNKECIKLCKP 342

Query: 301 GATIRDIHNYSVQMLRKGFKELGILQNWKSESYHQLNPTSIGHYLGMDVHDCSMVGYDRP 360
           GATI +IHNYSV+ LRKGFKELGILQNWKS+ YH LNPTSIGHYLGMDVHDC+ VGYDRP
Sbjct: 343 GATILEIHNYSVETLRKGFKELGILQNWKSDFYHHLNPTSIGHYLGMDVHDCARVGYDRP 402

Query: 361 LKPGVVITIEPGVYIPSLFEGPERFAGIGIRIEDEVLITESGHEVLTGSIPKEIKQIESL 420
           LKPGVVITIEPGVY+PS FEG ERFAGIGIRIEDEVLITESGHEVLTGSIPKEI QIESL
Sbjct: 403 LKPGVVITIEPGVYVPSSFEGSERFAGIGIRIEDEVLITESGHEVLTGSIPKEINQIESL 462

Query: 421 LNNFSFGS-VNSFNCMKAST 440
           LNNF FGS ++  N MKAST
Sbjct: 463 LNNFCFGSTMHGLNFMKAST 482

BLAST of MS007242 vs. NCBI nr
Match: KAA0048081.1 (putative Xaa-Pro aminopeptidase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 778.1 bits (2008), Expect = 4.0e-221
Identity = 377/440 (85.68%), Postives = 412/440 (93.64%), Query Frame = 0

Query: 1   MKEGEITPGITAEEYITRRKRLLELLPEKSVAIIAAAPVKMMTDVVPYTFRQDADYLYVT 60
           MKEGEITPGITAEEYITRRKRLLELLPE S+AIIAAAPVKMMTDVVPYTFRQD+DYLYVT
Sbjct: 43  MKEGEITPGITAEEYITRRKRLLELLPEDSLAIIAAAPVKMMTDVVPYTFRQDSDYLYVT 102

Query: 61  GCLQQGGLAVLSREFGFCMFMPEAKPHDVLWQGKIAGVDAALDFFKADEAYPMKKLREIL 120
           GCLQ GGLAVLS +FG CMFMPE KP+DVLW GKIAGVD AL+FFKADEAYP++K+REIL
Sbjct: 103 GCLQPGGLAVLSHDFGLCMFMPETKPYDVLWNGKIAGVDEALEFFKADEAYPLRKMREIL 162

Query: 121 PDIIRRSTKLFHNSTTATPAYVGLEAFQKAAENNRVQDISLLTHELRLIKSTAEMKLMRD 180
           PDIIRRS+KLFHNS TA+PAYV LEAFQKAAEN +V+DISL THELRL KSTAEMKLM++
Sbjct: 163 PDIIRRSSKLFHNSITASPAYVDLEAFQKAAENGQVKDISLFTHELRLTKSTAEMKLMKE 222

Query: 181 SASIACQALLQTMMHSKTYPYESKLSAKVEYECRMRGSQRMAFNPVTGGGSNGSVIHYSR 240
           SASIACQALLQTMMHSKTYP+ES LSAKVEYECRMRG+QRMA+NPV GGG NGSVIHYSR
Sbjct: 223 SASIACQALLQTMMHSKTYPHESLLSAKVEYECRMRGAQRMAYNPVVGGGCNGSVIHYSR 282

Query: 241 NDQKVREGDLVLMDIGCELHGYVSDLTRTWPPCGNFSAAQEELYDLILQTNKECIKLCKP 300
           ND+KV+EGDLVLMDIGCELHGYVSDLTRTWPPCG FSAAQEELY+LIL TNKECIKLCKP
Sbjct: 283 NDRKVKEGDLVLMDIGCELHGYVSDLTRTWPPCGKFSAAQEELYELILLTNKECIKLCKP 342

Query: 301 GATIRDIHNYSVQMLRKGFKELGILQNWKSESYHQLNPTSIGHYLGMDVHDCSMVGYDRP 360
           GA+I+ IHNY+ +MLR+GFKELGIL+N KS+ +H LNPTSIGHYLGMDVHDC+ VGYDRP
Sbjct: 343 GASIQHIHNYATKMLREGFKELGILKNSKSDLFHHLNPTSIGHYLGMDVHDCTRVGYDRP 402

Query: 361 LKPGVVITIEPGVYIPSLFEGPERFAGIGIRIEDEVLITESGHEVLTGSIPKEIKQIESL 420
           LKPGVVITIEPGVYIPS+F+GPERFAGIGIRIEDEVLITESG+EVLTGSIPKEI QIESL
Sbjct: 403 LKPGVVITIEPGVYIPSIFQGPERFAGIGIRIEDEVLITESGYEVLTGSIPKEINQIESL 462

Query: 421 LNNFSFGS-VNSFNCMKAST 440
           LNNF FGS +++ N MKAST
Sbjct: 463 LNNFCFGSTMHAPNFMKAST 482

BLAST of MS007242 vs. ExPASy Swiss-Prot
Match: F4HZG9 (Intermediate cleaving peptidase 55, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=ICP55 PE=1 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 1.0e-182
Identity = 305/423 (72.10%), Postives = 360/423 (85.11%), Query Frame = 0

Query: 1   MKEGEITPGITAEEYITRRKRLLELLPEKSVAIIAAAPVKMMTDVVPYTFRQDADYLYVT 60
           M EGE+TPGI  EEYI RRK+L+ELLPE S+AII++APVKMMTDVVPYTFRQDADYLY+T
Sbjct: 43  MAEGEVTPGIRIEEYIGRRKKLVELLPENSLAIISSAPVKMMTDVVPYTFRQDADYLYLT 102

Query: 61  GCLQQGGLAVLSREFGFCMFMPEAKPHDVLWQGKIAGVDAALDFFKADEAYPMKKLREIL 120
           GC Q GG+AVLS E G CMFMPE+ P D+ W+G++AGVDAA + FKAD+AYP+ KL EIL
Sbjct: 103 GCQQPGGVAVLSDERGLCMFMPESTPKDIAWEGEVAGVDAASEVFKADQAYPISKLPEIL 162

Query: 121 PDIIRRSTKLFHNSTTATPAYVGLEAFQKAAENNRVQDISLLTHELRLIKSTAEMKLMRD 180
            D+IR S+K+FHN  +A+  Y  L+ FQ +A   +V+ +S LTHELRLIKS AE+KLMR+
Sbjct: 163 SDMIRHSSKVFHNVQSASQRYTNLDDFQNSASLGKVKTLSSLTHELRLIKSPAELKLMRE 222

Query: 181 SASIACQALLQTMMHSKTYPYESKLSAKVEYECRMRGSQRMAFNPVTGGGSNGSVIHYSR 240
           SASIACQ LL+TM+HSK +P E  LSA+VEYECR+RG+QRMAFNPV GGGSN SVIHYSR
Sbjct: 223 SASIACQGLLKTMLHSKGFPDEGILSAQVEYECRVRGAQRMAFNPVVGGGSNASVIHYSR 282

Query: 241 NDQKVREGDLVLMDIGCELHGYVSDLTRTWPPCGNFSAAQEELYDLILQTNKECIKLCKP 300
           NDQ++++GDLVLMD+GCELHGYVSDLTRTWPPCG FS+ QEELYDLILQTNKECIK CKP
Sbjct: 283 NDQRIKDGDLVLMDMGCELHGYVSDLTRTWPPCGKFSSVQEELYDLILQTNKECIKQCKP 342

Query: 301 GATIRDIHNYSVQMLRKGFKELGILQNWKSESYHQLNPTSIGHYLGMDVHDCSMVGYDRP 360
           G TIR ++ YS ++L  G  ++GIL++     YHQLNPTSIGHYLGMDVHD S VGYDRP
Sbjct: 343 GTTIRQLNTYSTELLCDGLMKMGILKS--RRLYHQLNPTSIGHYLGMDVHDSSAVGYDRP 402

Query: 361 LKPGVVITIEPGVYIPSLFEGPERFAGIGIRIEDEVLITESGHEVLTGSIPKEIKQIESL 420
           L+PG VITIEPGVYIPS F+ PERF GIGIRIED+VLITE+G+EVLTGS+PKEIK IE+L
Sbjct: 403 LQPGFVITIEPGVYIPSSFDCPERFQGIGIRIEDDVLITETGYEVLTGSMPKEIKHIETL 462

Query: 421 LNN 424
           LNN
Sbjct: 463 LNN 463

BLAST of MS007242 vs. ExPASy Swiss-Prot
Match: B5DEQ3 (Xaa-Pro aminopeptidase 3 OS=Rattus norvegicus OX=10116 GN=Xpnpep3 PE=1 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 2.1e-87
Identity = 159/449 (35.41%), Postives = 266/449 (59.24%), Query Frame = 0

Query: 1   MKEGEITPGITAEEYITRRKRLLELLPEKS--------VAIIAAAPVKMMTDVVPYTFRQ 60
           ++ GE+TPG++  EY  RR +L+ L+ +++          ++ + P+  M++ +PYTF Q
Sbjct: 58  LRPGEVTPGLSQVEYALRRHKLMALVHKEAQGHSGTDHTVVVLSNPIHYMSNDIPYTFHQ 117

Query: 61  DADYLYVTGCLQQGGLAVLSREFG-------FCMFMPEAKPHDVLWQGKIAGVDAALDFF 120
           D  +LY+ G  +   + VL    G         +F+P   P   LW G  +G D A+   
Sbjct: 118 DNSFLYLCGFQEPDSILVLQSCSGKQLPSHKAMLFVPRRDPGRELWDGPRSGTDGAIALT 177

Query: 121 KADEAYPMKKLREILPDIIRRSTKLFHNSTTATPAYVGLEAFQ-----KAAENNRVQDIS 180
             D+AYP+++ + +LP +   +  ++++    + A +  +  Q     KA   N+V+ + 
Sbjct: 178 GVDDAYPLEEFQHLLPKLRAETNMVWYDWMKPSHAQLHSDYMQPLTEAKATSKNKVRSVQ 237

Query: 181 LLTHELRLIKSTAEMKLMRDSASIACQALLQTMMHSKTYPYESKLSAKVEYECRMRGSQR 240
            L   LRLIKS AE+K M+ +  +  +A ++TM  SK    E+ L AK E+ECR RG+  
Sbjct: 238 HLIQHLRLIKSPAEIKRMQIAGKLTSEAFIETMFASKAPVDEAFLYAKFEFECRARGADI 297

Query: 241 MAFNPVTGGGSNGSVIHYSRNDQKVREGDLVLMDIGCELHGYVSDLTRTWPPCGNFSAAQ 300
           +A+ PV  GG+  + +HY +N+Q +++G++VL+D GCE   YVSD+TRTWP  G F+A Q
Sbjct: 298 LAYPPVVAGGNRSNTLHYVKNNQLIKDGEMVLLDGGCESSCYVSDITRTWPVNGRFTAPQ 357

Query: 301 EELYDLILQTNKECIKLCKPGATIRDIHNYSVQMLRKGFKELGILQNWKSESY---HQLN 360
            ELY+ +L+  K C+ LC PG ++ +I++  + ++ +  K+LGI++  K  ++    +  
Sbjct: 358 AELYEAVLEIQKACLTLCSPGTSLENIYSMMLTLMGQKLKDLGIIKTSKESAFKAARKYC 417

Query: 361 PTSIGHYLGMDVHDCSMVGYDRPLKPGVVITIEPGVYIP-SLFEGPERFAGIGIRIEDEV 420
           P  +GHYLGMDVHD   +    PL+PG+VIT+EPG+YIP    + PE+F G+G+RIED+V
Sbjct: 418 PHHVGHYLGMDVHDTPDMPRSLPLQPGMVITVEPGIYIPEGDTDAPEKFRGLGVRIEDDV 477

Query: 421 LITESGHEVLTGSIPKEIKQIESLLNNFS 426
           ++T+    +L+   PKE+  IE + +  S
Sbjct: 478 VVTQDSPLILSADCPKEVNDIEQICSRTS 506

BLAST of MS007242 vs. ExPASy Swiss-Prot
Match: Q9NQH7 (Xaa-Pro aminopeptidase 3 OS=Homo sapiens OX=9606 GN=XPNPEP3 PE=1 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 4.0e-86
Identity = 160/450 (35.56%), Postives = 267/450 (59.33%), Query Frame = 0

Query: 1   MKEGEITPGITAEEYITRRKRLLELLPEKS--------VAIIAAAPVKMMTDVVPYTFRQ 60
           ++ GE+TPG++  EY  RR +L+ L+ +++          ++ + P   M++ +PYTF Q
Sbjct: 58  LRPGEVTPGLSQVEYALRRHKLMSLIQKEAQGQSGTDQTVVVLSNPTYYMSNDIPYTFHQ 117

Query: 61  DADYLYVTGCLQQGGLAVLSREFG-------FCMFMPEAKPHDVLWQGKIAGVDAALDFF 120
           D ++LY+ G  +   + VL    G         +F+P   P   LW G  +G D A+   
Sbjct: 118 DNNFLYLCGFQEPDSILVLQSLPGKQLPSHKAILFVPRRDPSRELWDGPRSGTDGAIALT 177

Query: 121 KADEAYPMKKLREILPDIIRRSTKLFHNSTTATPAYVGLEAFQ-----KAAENNRVQDIS 180
             DEAY +++ + +LP +   +  ++++    + A +  +  Q     KA   N+V+ + 
Sbjct: 178 GVDEAYTLEEFQHLLPKMKAETNMVWYDWMRPSHAQLHSDYMQPLTEAKAKSKNKVRGVQ 237

Query: 181 LLTHELRLIKSTAEMKLMRDSASIACQALLQTMMHSKTYPYESKLSAKVEYECRMRGSQR 240
            L   LRLIKS AE++ M+ +  +  QA ++TM  SK    E+ L AK E+ECR RG+  
Sbjct: 238 QLIQRLRLIKSPAEIERMQIAGKLTSQAFIETMFTSKAPVEEAFLYAKFEFECRARGADI 297

Query: 241 MAFNPVTGGGSNGSVIHYSRNDQKVREGDLVLMDIGCELHGYVSDLTRTWPPCGNFSAAQ 300
           +A+ PV  GG+  + +HY +N+Q +++G++VL+D GCE   YVSD+TRTWP  G F+A Q
Sbjct: 298 LAYPPVVAGGNRSNTLHYVKNNQLIKDGEMVLLDGGCESSCYVSDITRTWPVNGRFTAPQ 357

Query: 301 EELYDLILQTNKECIKLCKPGATIRDIHNYSVQMLRKGFKELGILQNWKS----ESYHQL 360
            ELY+ +L+  ++C+ LC PG ++ +I++  + ++ +  K+LGI++N K     ++  + 
Sbjct: 358 AELYEAVLEIQRDCLALCFPGTSLENIYSMMLTLIGQKLKDLGIMKNIKENNAFKAARKY 417

Query: 361 NPTSIGHYLGMDVHDCSMVGYDRPLKPGVVITIEPGVYIP-SLFEGPERFAGIGIRIEDE 420
            P  +GHYLGMDVHD   +    PL+PG+VITIEPG+YIP    + PE+F G+G+RIED+
Sbjct: 418 CPHHVGHYLGMDVHDTPDMPRSLPLQPGMVITIEPGIYIPEDDKDAPEKFRGLGVRIEDD 477

Query: 421 VLITESGHEVLTGSIPKEIKQIESLLNNFS 426
           V++T+    +L+   PKE+  IE + +  S
Sbjct: 478 VVVTQDSPLILSADCPKEMNDIEQICSQAS 507

BLAST of MS007242 vs. ExPASy Swiss-Prot
Match: Q5R9W8 (Xaa-Pro aminopeptidase 3 OS=Pongo abelii OX=9601 GN=XPNPEP3 PE=2 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 4.0e-86
Identity = 161/450 (35.78%), Postives = 266/450 (59.11%), Query Frame = 0

Query: 1   MKEGEITPGITAEEYITRRKRLLELLPEKS--------VAIIAAAPVKMMTDVVPYTFRQ 60
           ++ GE+TPG++  EY  RR +L+ L+ +++          ++ + P   M++ +PYTF Q
Sbjct: 58  LRPGEVTPGLSQVEYALRRHKLMSLIQKEAQGQSGTDQTVVVLSNPTYYMSNDIPYTFHQ 117

Query: 61  DADYLYVTGCLQQGGLAVLSREFG-------FCMFMPEAKPHDVLWQGKIAGVDAALDFF 120
           D ++LY+ G  +   + VL    G         +F+P   P   LW G  +G D A+   
Sbjct: 118 DNNFLYLCGFQEPDSILVLQSLPGKQLPSHKAILFVPRRDPSRELWDGPRSGTDGAIALT 177

Query: 121 KADEAYPMKKLREILPDIIRRSTKLFHNSTTATPAYVGLEAFQ-----KAAENNRVQDIS 180
             DEAY +++ + +LP +   +  ++++    + A +  +  Q     KA   N+V+ + 
Sbjct: 178 GVDEAYTLEEFQHLLPKMKAETNMVWYDWMRPSHAQLHSDYMQPLTEAKAKSKNKVRGVQ 237

Query: 181 LLTHELRLIKSTAEMKLMRDSASIACQALLQTMMHSKTYPYESKLSAKVEYECRMRGSQR 240
            L   LRLIKS AE++ M+ +  +  QA ++TM  SK    E  L AK E+ECR RG+  
Sbjct: 238 QLIQRLRLIKSPAEIERMQIAGKLTSQAFIETMFASKAPVEEGFLYAKFEFECRARGADI 297

Query: 241 MAFNPVTGGGSNGSVIHYSRNDQKVREGDLVLMDIGCELHGYVSDLTRTWPPCGNFSAAQ 300
           +A+ PV  GG+  + +HY +N+Q +++G++VL+D GCE   YVSD+TRTWP  G F+A Q
Sbjct: 298 LAYPPVVAGGNRSNTLHYVKNNQLIKDGEMVLLDGGCESSCYVSDITRTWPVNGRFTAPQ 357

Query: 301 EELYDLILQTNKECIKLCKPGATIRDIHNYSVQMLRKGFKELGILQNWKS----ESYHQL 360
            ELY+ IL+  ++C+ LC PG ++ +I++  + ++ +  K+LGI++N K     ++  + 
Sbjct: 358 AELYEAILEIQRDCLALCFPGTSLENIYSMMLTLIGQKLKDLGIMKNIKENNAFKAARKY 417

Query: 361 NPTSIGHYLGMDVHDCSMVGYDRPLKPGVVITIEPGVYIP-SLFEGPERFAGIGIRIEDE 420
            P  +GHYLGMDVHD   +    PL+PG+VITIEPG+YIP    + PE+F G+G+RIED+
Sbjct: 418 CPHHVGHYLGMDVHDTPDMPRSLPLQPGMVITIEPGIYIPEDDKDAPEKFRGLGVRIEDD 477

Query: 421 VLITESGHEVLTGSIPKEIKQIESLLNNFS 426
           V++T+    +L+   PKE+  IE + +  S
Sbjct: 478 VVVTQDSPLILSADCPKEMNDIEQICSRAS 507

BLAST of MS007242 vs. ExPASy Swiss-Prot
Match: B7ZMP1 (Xaa-Pro aminopeptidase 3 OS=Mus musculus OX=10090 GN=Xpnpep3 PE=1 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 6.7e-86
Identity = 157/449 (34.97%), Postives = 265/449 (59.02%), Query Frame = 0

Query: 1   MKEGEITPGITAEEYITRRKRLLELLPEKS--------VAIIAAAPVKMMTDVVPYTFRQ 60
           ++ GE+TPG++  EY  RR +L+ L+ +++          ++ + P   M++ +PYTF Q
Sbjct: 58  LRPGEVTPGLSQVEYALRRHKLMALVHKEAQGHSGTDHTVVVLSNPTYYMSNDIPYTFHQ 117

Query: 61  DADYLYVTGCLQQGGLAVLSREFG-------FCMFMPEAKPHDVLWQGKIAGVDAALDFF 120
           D ++LY+ G  +   + VL    G         +F+P   P   LW G  +G D A+   
Sbjct: 118 DNNFLYLCGFQEPDSILVLQSFSGKQLPSHKAMLFVPRRDPGRELWDGPRSGTDGAIALT 177

Query: 121 KADEAYPMKKLREILPDIIRRSTKLFHNSTTATPAYVGLEAFQ-----KAAENNRVQDIS 180
             DEAYP+++ + +LP +   +  ++++    + A +  +  Q     KA   N+V+ + 
Sbjct: 178 GVDEAYPLEEFQHLLPKLRAETNMVWYDWMKPSHAQLHSDYMQPLTEAKARSKNKVRSVQ 237

Query: 181 LLTHELRLIKSTAEMKLMRDSASIACQALLQTMMHSKTYPYESKLSAKVEYECRMRGSQR 240
            L   LRL+KS +E+K M+ +  +  +A ++TM  SK    E+ L AK E+ECR RG+  
Sbjct: 238 QLIQRLRLVKSPSEIKRMQIAGKLTSEAFIETMFASKAPIDEAFLYAKFEFECRARGADI 297

Query: 241 MAFNPVTGGGSNGSVIHYSRNDQKVREGDLVLMDIGCELHGYVSDLTRTWPPCGNFSAAQ 300
           +A+ PV  GG+  + +HY +N+Q +++G++VL+D GCE   YVSD+TRTWP  G F+A Q
Sbjct: 298 LAYPPVVAGGNRSNTLHYVKNNQLIKDGEMVLLDGGCESSCYVSDITRTWPVNGRFTAPQ 357

Query: 301 EELYDLILQTNKECIKLCKPGATIRDIHNYSVQMLRKGFKELGILQNWKSESY---HQLN 360
            ELY+ +L+  + C+ LC PG ++ +I++  + ++ +  K+LGI +  K  ++    +  
Sbjct: 358 AELYEAVLEIQRACLTLCSPGTSLENIYSMMLTLIGQKLKDLGITKTSKESAFKAARKYC 417

Query: 361 PTSIGHYLGMDVHDCSMVGYDRPLKPGVVITIEPGVYIP-SLFEGPERFAGIGIRIEDEV 420
           P  +GHYLGMDVHD   +    PL+PG+VIT+EPG+YIP    + PE+F G+G+RIED+V
Sbjct: 418 PHHVGHYLGMDVHDTPDMPRSLPLQPGMVITVEPGIYIPEDDRDAPEKFRGLGVRIEDDV 477

Query: 421 LITESGHEVLTGSIPKEIKQIESLLNNFS 426
           ++T+    +L+   PKE+  IE + +  S
Sbjct: 478 VVTQDSPLILSADCPKEMNDIEQICSRTS 506

BLAST of MS007242 vs. ExPASy TrEMBL
Match: A0A6J1DTD2 (probable Xaa-Pro aminopeptidase 3 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023304 PE=4 SV=1)

HSP 1 Score: 892.5 bits (2305), Expect = 7.1e-256
Identity = 436/440 (99.09%), Postives = 439/440 (99.77%), Query Frame = 0

Query: 1   MKEGEITPGITAEEYITRRKRLLELLPEKSVAIIAAAPVKMMTDVVPYTFRQDADYLYVT 60
           MKEGEITPGITAEEYITRRKRLLELLPEKSVAIIAAAPVKMMTDVVPYTFRQDADYLY+T
Sbjct: 43  MKEGEITPGITAEEYITRRKRLLELLPEKSVAIIAAAPVKMMTDVVPYTFRQDADYLYIT 102

Query: 61  GCLQQGGLAVLSREFGFCMFMPEAKPHDVLWQGKIAGVDAALDFFKADEAYPMKKLREIL 120
           GCLQQGGLAVLSREFGFCMFMPEAKPHDVLWQGKIAGVDAALDFFKADEAYPMKKLREIL
Sbjct: 103 GCLQQGGLAVLSREFGFCMFMPEAKPHDVLWQGKIAGVDAALDFFKADEAYPMKKLREIL 162

Query: 121 PDIIRRSTKLFHNSTTATPAYVGLEAFQKAAENNRVQDISLLTHELRLIKSTAEMKLMRD 180
           PDIIRRSTKLFHNSTTATPAYVGLEAFQKAAENNRVQDISLLTHELRLIKSTAEMKLMRD
Sbjct: 163 PDIIRRSTKLFHNSTTATPAYVGLEAFQKAAENNRVQDISLLTHELRLIKSTAEMKLMRD 222

Query: 181 SASIACQALLQTMMHSKTYPYESKLSAKVEYECRMRGSQRMAFNPVTGGGSNGSVIHYSR 240
           SASIACQALLQTMMHSKTYPYESKLSAKVEYECRMRG+QRMAFNPVTGGGSNGSVIHYSR
Sbjct: 223 SASIACQALLQTMMHSKTYPYESKLSAKVEYECRMRGAQRMAFNPVTGGGSNGSVIHYSR 282

Query: 241 NDQKVREGDLVLMDIGCELHGYVSDLTRTWPPCGNFSAAQEELYDLILQTNKECIKLCKP 300
           NDQKVREGDLVLMDIGCELHGYVSDLTRTWPPCGNFSAAQEELYDLILQTNKECIKLCKP
Sbjct: 283 NDQKVREGDLVLMDIGCELHGYVSDLTRTWPPCGNFSAAQEELYDLILQTNKECIKLCKP 342

Query: 301 GATIRDIHNYSVQMLRKGFKELGILQNWKSESYHQLNPTSIGHYLGMDVHDCSMVGYDRP 360
           GATIRDIHNYSVQMLRKGFKELGILQNWKSESYHQLNPTSIGHYLGMDVHDCSMVGYDRP
Sbjct: 343 GATIRDIHNYSVQMLRKGFKELGILQNWKSESYHQLNPTSIGHYLGMDVHDCSMVGYDRP 402

Query: 361 LKPGVVITIEPGVYIPSLFEGPERFAGIGIRIEDEVLITESGHEVLTGSIPKEIKQIESL 420
           LK GVVITIEPGVYIPSLFEGPERFAGIGIRIED+VLITESGHEVLTGSIPKEIKQIESL
Sbjct: 403 LKXGVVITIEPGVYIPSLFEGPERFAGIGIRIEDDVLITESGHEVLTGSIPKEIKQIESL 462

Query: 421 LNNFSFGSVNSFNCMKASTF 441
           LNNFSFGSVNSFNCMKASTF
Sbjct: 463 LNNFSFGSVNSFNCMKASTF 482

BLAST of MS007242 vs. ExPASy TrEMBL
Match: A0A6J1FC96 (intermediate cleaving peptidase 55, mitochondrial isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444089 PE=4 SV=1)

HSP 1 Score: 789.3 bits (2037), Expect = 8.5e-225
Identity = 380/440 (86.36%), Postives = 407/440 (92.50%), Query Frame = 0

Query: 1   MKEGEITPGITAEEYITRRKRLLELLPEKSVAIIAAAPVKMMTDVVPYTFRQDADYLYVT 60
           + EGEITPGITAEEYITRRKRLLELLP+  +AIIAAAPVKMMTDVVPYTFRQDADYLY+T
Sbjct: 43  LNEGEITPGITAEEYITRRKRLLELLPQNGLAIIAAAPVKMMTDVVPYTFRQDADYLYIT 102

Query: 61  GCLQQGGLAVLSREFGFCMFMPEAKPHDVLWQGKIAGVDAALDFFKADEAYPMKKLREIL 120
           GCLQ GGLAV+S EFG CMFMPE KP+DVLWQG+ AGVDAAL+FFK D+AYPMKKLREIL
Sbjct: 103 GCLQPGGLAVMSHEFGLCMFMPETKPYDVLWQGQTAGVDAALEFFKGDQAYPMKKLREIL 162

Query: 121 PDIIRRSTKLFHNSTTATPAYVGLEAFQKAAENNRVQDISLLTHELRLIKSTAEMKLMRD 180
           PDIIRRS+KLFHNS TATP Y+ LEAF+KAAENN V+DISL THELRLIKSTAE KLMRD
Sbjct: 163 PDIIRRSSKLFHNSNTATPTYLDLEAFRKAAENNCVKDISLFTHELRLIKSTAEQKLMRD 222

Query: 181 SASIACQALLQTMMHSKTYPYESKLSAKVEYECRMRGSQRMAFNPVTGGGSNGSVIHYSR 240
           SASIACQALLQTM+HSKTYP+ES LSAKVEYECRMRG+QRMAFNPV GGG NGSVIHYSR
Sbjct: 223 SASIACQALLQTMIHSKTYPHESMLSAKVEYECRMRGAQRMAFNPVVGGGCNGSVIHYSR 282

Query: 241 NDQKVREGDLVLMDIGCELHGYVSDLTRTWPPCGNFSAAQEELYDLILQTNKECIKLCKP 300
           NDQKVR+GD VLMD+GCELHGYVSDLTRTWPPCG FSAAQEELY+LILQTNKECIKLCKP
Sbjct: 283 NDQKVRDGDFVLMDVGCELHGYVSDLTRTWPPCGKFSAAQEELYELILQTNKECIKLCKP 342

Query: 301 GATIRDIHNYSVQMLRKGFKELGILQNWKSESYHQLNPTSIGHYLGMDVHDCSMVGYDRP 360
           GATI +IHNYSV+ LRKGFKELGILQNWKS+ YH LNPTSIGHYLGMDVHDC+ VGYDRP
Sbjct: 343 GATILEIHNYSVETLRKGFKELGILQNWKSDFYHHLNPTSIGHYLGMDVHDCARVGYDRP 402

Query: 361 LKPGVVITIEPGVYIPSLFEGPERFAGIGIRIEDEVLITESGHEVLTGSIPKEIKQIESL 420
           LKPGVVITIEPGVY+PS FEG ERFAGIGIRIEDEVLITESGHEVLTGSIPKEI QIES+
Sbjct: 403 LKPGVVITIEPGVYVPSSFEGSERFAGIGIRIEDEVLITESGHEVLTGSIPKEINQIESM 462

Query: 421 LNNFSFGS-VNSFNCMKAST 440
           LNNF FGS ++  N MKAST
Sbjct: 463 LNNFCFGSTMHGLNFMKAST 482

BLAST of MS007242 vs. ExPASy TrEMBL
Match: A0A6J1HPR0 (intermediate cleaving peptidase 55, mitochondrial isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465520 PE=4 SV=1)

HSP 1 Score: 788.1 bits (2034), Expect = 1.9e-224
Identity = 381/440 (86.59%), Postives = 406/440 (92.27%), Query Frame = 0

Query: 1   MKEGEITPGITAEEYITRRKRLLELLPEKSVAIIAAAPVKMMTDVVPYTFRQDADYLYVT 60
           + EGEITPGITAEEYITRRKRLLELLP+  +AIIAAAPVKMMTDVVPYTFRQDADYLY+T
Sbjct: 43  LNEGEITPGITAEEYITRRKRLLELLPQNGLAIIAAAPVKMMTDVVPYTFRQDADYLYIT 102

Query: 61  GCLQQGGLAVLSREFGFCMFMPEAKPHDVLWQGKIAGVDAALDFFKADEAYPMKKLREIL 120
           GCLQ GGLAV+S EFG CMFMPE KP+DVLWQGKIAGVDAAL+FFKAD+AYPM KLREIL
Sbjct: 103 GCLQPGGLAVMSHEFGLCMFMPETKPYDVLWQGKIAGVDAALEFFKADQAYPMTKLREIL 162

Query: 121 PDIIRRSTKLFHNSTTATPAYVGLEAFQKAAENNRVQDISLLTHELRLIKSTAEMKLMRD 180
           PDIIRRS+KLFHNS TAT  Y+ LEAF+KAAENN V+DISL THELRLIKSTAE KLMRD
Sbjct: 163 PDIIRRSSKLFHNSNTATSTYLDLEAFRKAAENNCVKDISLFTHELRLIKSTAEQKLMRD 222

Query: 181 SASIACQALLQTMMHSKTYPYESKLSAKVEYECRMRGSQRMAFNPVTGGGSNGSVIHYSR 240
           SASIACQALLQTM+HSKTYP+ES LSAKVEYECRMRG+QRMAFNPV GGG NGSVIHYSR
Sbjct: 223 SASIACQALLQTMIHSKTYPHESMLSAKVEYECRMRGAQRMAFNPVVGGGCNGSVIHYSR 282

Query: 241 NDQKVREGDLVLMDIGCELHGYVSDLTRTWPPCGNFSAAQEELYDLILQTNKECIKLCKP 300
           NDQKVR+GD VLMD+GCELHGYVSDLTRTWPPCG FSA QEELY+LILQTNKECIKLCKP
Sbjct: 283 NDQKVRDGDFVLMDVGCELHGYVSDLTRTWPPCGKFSATQEELYELILQTNKECIKLCKP 342

Query: 301 GATIRDIHNYSVQMLRKGFKELGILQNWKSESYHQLNPTSIGHYLGMDVHDCSMVGYDRP 360
           GATI +IHNYSV+ LRKGFKELGILQNWKS+ YH LNPTSIGHYLGMDVHDC+ VGYDRP
Sbjct: 343 GATILEIHNYSVETLRKGFKELGILQNWKSDFYHHLNPTSIGHYLGMDVHDCARVGYDRP 402

Query: 361 LKPGVVITIEPGVYIPSLFEGPERFAGIGIRIEDEVLITESGHEVLTGSIPKEIKQIESL 420
           LKPGVVITIEPGVY+PS FEG ERFAGIGIRIEDEVLITESGHEVLTGSIPKEI QIESL
Sbjct: 403 LKPGVVITIEPGVYVPSSFEGSERFAGIGIRIEDEVLITESGHEVLTGSIPKEINQIESL 462

Query: 421 LNNFSFGS-VNSFNCMKAST 440
           LNNF FGS ++  N MKAST
Sbjct: 463 LNNFCFGSTMHGLNFMKAST 482

BLAST of MS007242 vs. ExPASy TrEMBL
Match: A0A5A7U3F1 (Putative Xaa-Pro aminopeptidase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G001530 PE=4 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 2.0e-221
Identity = 377/440 (85.68%), Postives = 412/440 (93.64%), Query Frame = 0

Query: 1   MKEGEITPGITAEEYITRRKRLLELLPEKSVAIIAAAPVKMMTDVVPYTFRQDADYLYVT 60
           MKEGEITPGITAEEYITRRKRLLELLPE S+AIIAAAPVKMMTDVVPYTFRQD+DYLYVT
Sbjct: 43  MKEGEITPGITAEEYITRRKRLLELLPEDSLAIIAAAPVKMMTDVVPYTFRQDSDYLYVT 102

Query: 61  GCLQQGGLAVLSREFGFCMFMPEAKPHDVLWQGKIAGVDAALDFFKADEAYPMKKLREIL 120
           GCLQ GGLAVLS +FG CMFMPE KP+DVLW GKIAGVD AL+FFKADEAYP++K+REIL
Sbjct: 103 GCLQPGGLAVLSHDFGLCMFMPETKPYDVLWNGKIAGVDEALEFFKADEAYPLRKMREIL 162

Query: 121 PDIIRRSTKLFHNSTTATPAYVGLEAFQKAAENNRVQDISLLTHELRLIKSTAEMKLMRD 180
           PDIIRRS+KLFHNS TA+PAYV LEAFQKAAEN +V+DISL THELRL KSTAEMKLM++
Sbjct: 163 PDIIRRSSKLFHNSITASPAYVDLEAFQKAAENGQVKDISLFTHELRLTKSTAEMKLMKE 222

Query: 181 SASIACQALLQTMMHSKTYPYESKLSAKVEYECRMRGSQRMAFNPVTGGGSNGSVIHYSR 240
           SASIACQALLQTMMHSKTYP+ES LSAKVEYECRMRG+QRMA+NPV GGG NGSVIHYSR
Sbjct: 223 SASIACQALLQTMMHSKTYPHESLLSAKVEYECRMRGAQRMAYNPVVGGGCNGSVIHYSR 282

Query: 241 NDQKVREGDLVLMDIGCELHGYVSDLTRTWPPCGNFSAAQEELYDLILQTNKECIKLCKP 300
           ND+KV+EGDLVLMDIGCELHGYVSDLTRTWPPCG FSAAQEELY+LIL TNKECIKLCKP
Sbjct: 283 NDRKVKEGDLVLMDIGCELHGYVSDLTRTWPPCGKFSAAQEELYELILLTNKECIKLCKP 342

Query: 301 GATIRDIHNYSVQMLRKGFKELGILQNWKSESYHQLNPTSIGHYLGMDVHDCSMVGYDRP 360
           GA+I+ IHNY+ +MLR+GFKELGIL+N KS+ +H LNPTSIGHYLGMDVHDC+ VGYDRP
Sbjct: 343 GASIQHIHNYATKMLREGFKELGILKNSKSDLFHHLNPTSIGHYLGMDVHDCTRVGYDRP 402

Query: 361 LKPGVVITIEPGVYIPSLFEGPERFAGIGIRIEDEVLITESGHEVLTGSIPKEIKQIESL 420
           LKPGVVITIEPGVYIPS+F+GPERFAGIGIRIEDEVLITESG+EVLTGSIPKEI QIESL
Sbjct: 403 LKPGVVITIEPGVYIPSIFQGPERFAGIGIRIEDEVLITESGYEVLTGSIPKEINQIESL 462

Query: 421 LNNFSFGS-VNSFNCMKAST 440
           LNNF FGS +++ N MKAST
Sbjct: 463 LNNFCFGSTMHAPNFMKAST 482

BLAST of MS007242 vs. ExPASy TrEMBL
Match: A0A5D3BC20 (Putative Xaa-Pro aminopeptidase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G00970 PE=4 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 2.0e-221
Identity = 377/440 (85.68%), Postives = 412/440 (93.64%), Query Frame = 0

Query: 1   MKEGEITPGITAEEYITRRKRLLELLPEKSVAIIAAAPVKMMTDVVPYTFRQDADYLYVT 60
           MKEGEITPGITAEEYITRRKRLLELLPE S+AIIAAAPVKMMTDVVPYTFRQD+DYLYVT
Sbjct: 150 MKEGEITPGITAEEYITRRKRLLELLPEDSLAIIAAAPVKMMTDVVPYTFRQDSDYLYVT 209

Query: 61  GCLQQGGLAVLSREFGFCMFMPEAKPHDVLWQGKIAGVDAALDFFKADEAYPMKKLREIL 120
           GCLQ GGLAVLS +FG CMFMPE KP+DVLW GKIAGVD AL+FFKADEAYP++K+REIL
Sbjct: 210 GCLQPGGLAVLSHDFGLCMFMPETKPYDVLWNGKIAGVDEALEFFKADEAYPLRKMREIL 269

Query: 121 PDIIRRSTKLFHNSTTATPAYVGLEAFQKAAENNRVQDISLLTHELRLIKSTAEMKLMRD 180
           PDIIRRS+KLFHNS TA+PAYV LEAFQKAAEN +V+DISL THELRL KSTAEMKLM++
Sbjct: 270 PDIIRRSSKLFHNSITASPAYVDLEAFQKAAENGQVKDISLFTHELRLTKSTAEMKLMKE 329

Query: 181 SASIACQALLQTMMHSKTYPYESKLSAKVEYECRMRGSQRMAFNPVTGGGSNGSVIHYSR 240
           SASIACQALLQTMMHSKTYP+ES LSAKVEYECRMRG+QRMA+NPV GGG NGSVIHYSR
Sbjct: 330 SASIACQALLQTMMHSKTYPHESLLSAKVEYECRMRGAQRMAYNPVVGGGCNGSVIHYSR 389

Query: 241 NDQKVREGDLVLMDIGCELHGYVSDLTRTWPPCGNFSAAQEELYDLILQTNKECIKLCKP 300
           ND+KV+EGDLVLMDIGCELHGYVSDLTRTWPPCG FSAAQEELY+LIL TNKECIKLCKP
Sbjct: 390 NDRKVKEGDLVLMDIGCELHGYVSDLTRTWPPCGKFSAAQEELYELILLTNKECIKLCKP 449

Query: 301 GATIRDIHNYSVQMLRKGFKELGILQNWKSESYHQLNPTSIGHYLGMDVHDCSMVGYDRP 360
           GA+I+ IHNY+ +MLR+GFKELGIL+N KS+ +H LNPTSIGHYLGMDVHDC+ VGYDRP
Sbjct: 450 GASIQHIHNYATKMLREGFKELGILKNSKSDLFHHLNPTSIGHYLGMDVHDCTRVGYDRP 509

Query: 361 LKPGVVITIEPGVYIPSLFEGPERFAGIGIRIEDEVLITESGHEVLTGSIPKEIKQIESL 420
           LKPGVVITIEPGVYIPS+F+GPERFAGIGIRIEDEVLITESG+EVLTGSIPKEI QIESL
Sbjct: 510 LKPGVVITIEPGVYIPSIFQGPERFAGIGIRIEDEVLITESGYEVLTGSIPKEINQIESL 569

Query: 421 LNNFSFGS-VNSFNCMKAST 440
           LNNF FGS +++ N MKAST
Sbjct: 570 LNNFCFGSTMHAPNFMKAST 589

BLAST of MS007242 vs. TAIR 10
Match: AT1G09300.1 (Metallopeptidase M24 family protein )

HSP 1 Score: 641.0 bits (1652), Expect = 7.2e-184
Identity = 305/423 (72.10%), Postives = 360/423 (85.11%), Query Frame = 0

Query: 1   MKEGEITPGITAEEYITRRKRLLELLPEKSVAIIAAAPVKMMTDVVPYTFRQDADYLYVT 60
           M EGE+TPGI  EEYI RRK+L+ELLPE S+AII++APVKMMTDVVPYTFRQDADYLY+T
Sbjct: 43  MAEGEVTPGIRIEEYIGRRKKLVELLPENSLAIISSAPVKMMTDVVPYTFRQDADYLYLT 102

Query: 61  GCLQQGGLAVLSREFGFCMFMPEAKPHDVLWQGKIAGVDAALDFFKADEAYPMKKLREIL 120
           GC Q GG+AVLS E G CMFMPE+ P D+ W+G++AGVDAA + FKAD+AYP+ KL EIL
Sbjct: 103 GCQQPGGVAVLSDERGLCMFMPESTPKDIAWEGEVAGVDAASEVFKADQAYPISKLPEIL 162

Query: 121 PDIIRRSTKLFHNSTTATPAYVGLEAFQKAAENNRVQDISLLTHELRLIKSTAEMKLMRD 180
            D+IR S+K+FHN  +A+  Y  L+ FQ +A   +V+ +S LTHELRLIKS AE+KLMR+
Sbjct: 163 SDMIRHSSKVFHNVQSASQRYTNLDDFQNSASLGKVKTLSSLTHELRLIKSPAELKLMRE 222

Query: 181 SASIACQALLQTMMHSKTYPYESKLSAKVEYECRMRGSQRMAFNPVTGGGSNGSVIHYSR 240
           SASIACQ LL+TM+HSK +P E  LSA+VEYECR+RG+QRMAFNPV GGGSN SVIHYSR
Sbjct: 223 SASIACQGLLKTMLHSKGFPDEGILSAQVEYECRVRGAQRMAFNPVVGGGSNASVIHYSR 282

Query: 241 NDQKVREGDLVLMDIGCELHGYVSDLTRTWPPCGNFSAAQEELYDLILQTNKECIKLCKP 300
           NDQ++++GDLVLMD+GCELHGYVSDLTRTWPPCG FS+ QEELYDLILQTNKECIK CKP
Sbjct: 283 NDQRIKDGDLVLMDMGCELHGYVSDLTRTWPPCGKFSSVQEELYDLILQTNKECIKQCKP 342

Query: 301 GATIRDIHNYSVQMLRKGFKELGILQNWKSESYHQLNPTSIGHYLGMDVHDCSMVGYDRP 360
           G TIR ++ YS ++L  G  ++GIL++     YHQLNPTSIGHYLGMDVHD S VGYDRP
Sbjct: 343 GTTIRQLNTYSTELLCDGLMKMGILKS--RRLYHQLNPTSIGHYLGMDVHDSSAVGYDRP 402

Query: 361 LKPGVVITIEPGVYIPSLFEGPERFAGIGIRIEDEVLITESGHEVLTGSIPKEIKQIESL 420
           L+PG VITIEPGVYIPS F+ PERF GIGIRIED+VLITE+G+EVLTGS+PKEIK IE+L
Sbjct: 403 LQPGFVITIEPGVYIPSSFDCPERFQGIGIRIEDDVLITETGYEVLTGSMPKEIKHIETL 462

Query: 421 LNN 424
           LNN
Sbjct: 463 LNN 463

BLAST of MS007242 vs. TAIR 10
Match: AT1G09300.2 (Metallopeptidase M24 family protein )

HSP 1 Score: 641.0 bits (1652), Expect = 7.2e-184
Identity = 305/423 (72.10%), Postives = 360/423 (85.11%), Query Frame = 0

Query: 1   MKEGEITPGITAEEYITRRKRLLELLPEKSVAIIAAAPVKMMTDVVPYTFRQDADYLYVT 60
           M EGE+TPGI  EEYI RRK+L+ELLPE S+AII++APVKMMTDVVPYTFRQDADYLY+T
Sbjct: 12  MAEGEVTPGIRIEEYIGRRKKLVELLPENSLAIISSAPVKMMTDVVPYTFRQDADYLYLT 71

Query: 61  GCLQQGGLAVLSREFGFCMFMPEAKPHDVLWQGKIAGVDAALDFFKADEAYPMKKLREIL 120
           GC Q GG+AVLS E G CMFMPE+ P D+ W+G++AGVDAA + FKAD+AYP+ KL EIL
Sbjct: 72  GCQQPGGVAVLSDERGLCMFMPESTPKDIAWEGEVAGVDAASEVFKADQAYPISKLPEIL 131

Query: 121 PDIIRRSTKLFHNSTTATPAYVGLEAFQKAAENNRVQDISLLTHELRLIKSTAEMKLMRD 180
            D+IR S+K+FHN  +A+  Y  L+ FQ +A   +V+ +S LTHELRLIKS AE+KLMR+
Sbjct: 132 SDMIRHSSKVFHNVQSASQRYTNLDDFQNSASLGKVKTLSSLTHELRLIKSPAELKLMRE 191

Query: 181 SASIACQALLQTMMHSKTYPYESKLSAKVEYECRMRGSQRMAFNPVTGGGSNGSVIHYSR 240
           SASIACQ LL+TM+HSK +P E  LSA+VEYECR+RG+QRMAFNPV GGGSN SVIHYSR
Sbjct: 192 SASIACQGLLKTMLHSKGFPDEGILSAQVEYECRVRGAQRMAFNPVVGGGSNASVIHYSR 251

Query: 241 NDQKVREGDLVLMDIGCELHGYVSDLTRTWPPCGNFSAAQEELYDLILQTNKECIKLCKP 300
           NDQ++++GDLVLMD+GCELHGYVSDLTRTWPPCG FS+ QEELYDLILQTNKECIK CKP
Sbjct: 252 NDQRIKDGDLVLMDMGCELHGYVSDLTRTWPPCGKFSSVQEELYDLILQTNKECIKQCKP 311

Query: 301 GATIRDIHNYSVQMLRKGFKELGILQNWKSESYHQLNPTSIGHYLGMDVHDCSMVGYDRP 360
           G TIR ++ YS ++L  G  ++GIL++     YHQLNPTSIGHYLGMDVHD S VGYDRP
Sbjct: 312 GTTIRQLNTYSTELLCDGLMKMGILKS--RRLYHQLNPTSIGHYLGMDVHDSSAVGYDRP 371

Query: 361 LKPGVVITIEPGVYIPSLFEGPERFAGIGIRIEDEVLITESGHEVLTGSIPKEIKQIESL 420
           L+PG VITIEPGVYIPS F+ PERF GIGIRIED+VLITE+G+EVLTGS+PKEIK IE+L
Sbjct: 372 LQPGFVITIEPGVYIPSSFDCPERFQGIGIRIEDDVLITETGYEVLTGSMPKEIKHIETL 431

Query: 421 LNN 424
           LNN
Sbjct: 432 LNN 432

BLAST of MS007242 vs. TAIR 10
Match: AT4G29490.1 (Metallopeptidase M24 family protein )

HSP 1 Score: 142.5 bits (358), Expect = 8.0e-34
Identity = 107/428 (25.00%), Postives = 201/428 (46.96%), Query Frame = 0

Query: 50  FRQDADYLYVTGCLQQGGLAVLSREFG-FCMFMPEAKPHDVLWQGKIAGVDAALDFFKAD 109
           FRQ++ + Y+ G  +      +    G   +F+P       +W G+I  +    + +  D
Sbjct: 59  FRQESYFAYLFGVREPDFYGAIDIGSGKSILFIPRLPDDYAVWLGEIKPLSHFKETYMVD 118

Query: 110 EAYPMKKLREILPDIIRRSTK----LFH-----NSTTATPA-YVGLEAFQKAAENNRVQD 169
             + + ++ ++  +  + S K    L H     +S  + PA + G++ F+       +  
Sbjct: 119 MVFYVDEIIQVFNEQFKGSGKPLLYLLHGLNTDSSNFSKPASFEGIDKFE-----TDLTT 178

Query: 170 ISLLTHELRLIKSTAEMKLMRDSASIACQALLQTMMH----SKTYPYESKLSAKVEYECR 229
           +  +  E R+IKS+ E++L++ +  I+ +A ++ M       K Y  ES     + +   
Sbjct: 179 LHPILAECRVIKSSLELQLIQFANDISSEAHIEVMRKVTPGMKEYQMESMF---LHHSYM 238

Query: 230 MRGSQRMAFNPVTGGGSNGSVIHYSR----NDQKVREGDLVLMDIGCELHGYVSDLTRTW 289
             G +  ++  +   G N +V+HY      ND+   +GDL L+D+G E H Y SD+T ++
Sbjct: 239 YGGCRHCSYTCICATGDNSAVLHYGHAAAPNDRTFEDGDLALLDMGAEYHFYGSDITCSF 298

Query: 290 PPCGNFSAAQEELYDLILQTNKECIKLCKPGATIRDIHNYSVQMLRKGFKELGILQNWKS 349
           P  G F++ Q  +Y+ +L  +   I   KPG    D+H  + +++ +  K+  IL     
Sbjct: 299 PVNGKFTSDQSLIYNAVLDAHNSVISAMKPGVNWVDMHKLAEKIILESLKKGSILTGDVD 358

Query: 350 ESYHQ-----LNPTSIGHYLGMDVHDCS--MVGYDRPLKP-------------GVVITIE 409
           +   Q       P  +GH++G+D HD      G +RP KP             G+VIT+E
Sbjct: 359 DMMVQRLGAVFMPHGLGHFMGIDTHDTGGYPKGVERPKKPGLKSLRTARDLLEGMVITVE 418

Query: 410 PGVY------IPSLF----------EGPERFAGI-GIRIEDEVLITESGHEVLTGSIPKE 422
           PG Y       P++           E  ERF    G+RIE ++++T +G + +T ++P+E
Sbjct: 419 PGCYFIKALLFPAMANATTSKFFNRETIERFRNFGGVRIESDLVVTANGCKNMT-NVPRE 477

BLAST of MS007242 vs. TAIR 10
Match: AT3G05350.1 (Metallopeptidase M24 family protein )

HSP 1 Score: 59.3 bits (142), Expect = 8.9e-09
Identity = 75/298 (25.17%), Postives = 127/298 (42.62%), Query Frame = 0

Query: 167 RLIKSTAEMKLM-----RDSASIA-CQALLQTMMHSKTYPYESKLSAK-VEYECRMRGSQ 226
           + IK+ AE+K M     RD+A++A   A L+  +H      E  ++ + +E+     G  
Sbjct: 409 KAIKNDAELKGMKNSHLRDAAALAHFWAWLEEEVHKNANLTEVDVADRLLEFRSMQDGFM 468

Query: 227 RMAFNPVTGGGSNGSVIHYSRNDQKVREGD---LVLMDIGCELHGYVSDLTRTWPPCGNF 286
             +F+ ++G G+NG++IHY    +     D   L L+D G +     +D+TRT       
Sbjct: 469 DTSFDTISGSGANGAIIHYKPEPESCSRVDPQKLFLLDSGAQYVDGTTDITRT-VHFSEP 528

Query: 287 SAAQEELYDLILQTNKECIKLCKPGATIRDIHNYSVQMLRKGFKELGILQN--WKSESYH 346
           SA ++E +  +LQ +    +   P  T              GF   G  ++  WK    +
Sbjct: 529 SAREKECFTRVLQGHIALDQAVFPEGT-------------PGFVLDGFARSSLWKIGLDY 588

Query: 347 QLNPTSIGHYLGMDVHD-----CSMVGYDRPLKPGVVITIEPGVYIPSLF---------- 406
           + + T  G    ++VH+         G   PL+ G++++ EPG Y    F          
Sbjct: 589 R-HGTGHGVGAALNVHEGPQSISFRYGNMTPLQNGMIVSNEPGYYEDHAFGIRIENLLHV 648

Query: 407 ---EGPERFAGIGIRIEDEVLITESGHEVLTGSIPKEIKQIE-SLLNNFSFGSVNSFN 434
              E P RF G           T  G E LT   P + K ++ SLL++     +NS++
Sbjct: 649 RDAETPNRFGG----------ATYLGFEKLT-FFPIQTKMVDVSLLSDTEVDWLNSYH 680

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022156404.11.5e-25599.09probable Xaa-Pro aminopeptidase 3 isoform X1 [Momordica charantia][more]
XP_023537266.17.9e-22586.59intermediate cleaving peptidase 55, mitochondrial isoform X1 [Cucurbita pepo sub... [more]
XP_022937804.11.8e-22486.36intermediate cleaving peptidase 55, mitochondrial isoform X1 [Cucurbita moschata... [more]
XP_022965697.13.9e-22486.59intermediate cleaving peptidase 55, mitochondrial isoform X1 [Cucurbita maxima] ... [more]
KAA0048081.14.0e-22185.68putative Xaa-Pro aminopeptidase 3 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
F4HZG91.0e-18272.10Intermediate cleaving peptidase 55, mitochondrial OS=Arabidopsis thaliana OX=370... [more]
B5DEQ32.1e-8735.41Xaa-Pro aminopeptidase 3 OS=Rattus norvegicus OX=10116 GN=Xpnpep3 PE=1 SV=1[more]
Q9NQH74.0e-8635.56Xaa-Pro aminopeptidase 3 OS=Homo sapiens OX=9606 GN=XPNPEP3 PE=1 SV=1[more]
Q5R9W84.0e-8635.78Xaa-Pro aminopeptidase 3 OS=Pongo abelii OX=9601 GN=XPNPEP3 PE=2 SV=1[more]
B7ZMP16.7e-8634.97Xaa-Pro aminopeptidase 3 OS=Mus musculus OX=10090 GN=Xpnpep3 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DTD27.1e-25699.09probable Xaa-Pro aminopeptidase 3 isoform X1 OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1FC968.5e-22586.36intermediate cleaving peptidase 55, mitochondrial isoform X1 OS=Cucurbita moscha... [more]
A0A6J1HPR01.9e-22486.59intermediate cleaving peptidase 55, mitochondrial isoform X1 OS=Cucurbita maxima... [more]
A0A5A7U3F12.0e-22185.68Putative Xaa-Pro aminopeptidase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A5D3BC202.0e-22185.68Putative Xaa-Pro aminopeptidase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
Match NameE-valueIdentityDescription
AT1G09300.17.2e-18472.10Metallopeptidase M24 family protein [more]
AT1G09300.27.2e-18472.10Metallopeptidase M24 family protein [more]
AT4G29490.18.0e-3425.00Metallopeptidase M24 family protein [more]
AT3G05350.18.9e-0925.17Metallopeptidase M24 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001714Peptidase M24, methionine aminopeptidasePRINTSPR00599MAPEPTIDASEcoord: 249..265
score: 36.9
coord: 359..371
score: 46.15
IPR007865Aminopeptidase P, N-terminalSMARTSM01011AMP_N_2coord: 10..141
e-value: 4.6E-34
score: 129.2
IPR007865Aminopeptidase P, N-terminalPFAMPF05195AMP_Ncoord: 15..129
e-value: 2.2E-30
score: 104.9
IPR036005Creatinase/aminopeptidase-likeGENE3D3.90.230.10Creatinase/methionine aminopeptidase superfamilycoord: 169..430
e-value: 1.7E-83
score: 281.8
IPR036005Creatinase/aminopeptidase-likeSUPERFAMILY55920Creatinase/aminopeptidasecoord: 171..422
IPR000994Peptidase M24PFAMPF00557Peptidase_M24coord: 177..400
e-value: 3.3E-53
score: 180.5
IPR029149Creatinase/Aminopeptidase P/Spt16, N-terminalGENE3D3.40.350.10coord: 9..167
e-value: 5.5E-35
score: 122.6
IPR029149Creatinase/Aminopeptidase P/Spt16, N-terminalSUPERFAMILY53092Creatinase/prolidase N-terminal domaincoord: 8..170
NoneNo IPR availablePANTHERPTHR43226:SF4XAA-PRO AMINOPEPTIDASE 3coord: 2..423
NoneNo IPR availablePANTHERPTHR43226XAA-PRO AMINOPEPTIDASE 3coord: 2..423
NoneNo IPR availableCDDcd01087Prolidasecoord: 175..408
e-value: 3.73229E-102
score: 302.184

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS007242.1MS007242.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0030145 manganese ion binding
molecular_function GO:0070006 metalloaminopeptidase activity