MS007215 (gene) Bitter gourd (TR) v1

Overview
NameMS007215
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionleucine aminopeptidase 2, chloroplastic-like
Locationscaffold25: 1501436 .. 1506865 (-)
RNA-Seq ExpressionMS007215
SyntenyMS007215
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTACCTGTTTGTAAAAATGTCTCAAAGATAAAATTTGATGCAAAACGCCTGGATGAGTTGGAATGGAATGGAGACCTACTAGCTGTGGGCGTGACACAGGAAGATGTGGCCAAGGATGAAAACTCCATCTTCGAGAATCCACTTTTGTACAGGTTGGATGCAAGGTTGAATGGGCTGCTGGCTGAAGCTTGTTCAGAGGAAGATTTCTCTGGAAAATCTGGCCAATCTCTTGTTCTAAGAGTTTCTGGTCTTGCCTCTAAGAGGGTTGGTTTATTTGGCCTTGGACAGTCAGCTTCTACTGTTGCAGCCATTCAAGGTCTAGGTGAGGCCATTGCAGCAGCAGCAAAGGCATCTCGAGCAAGTTCAGTTGCTGTATCTCTTGCCTTTTCCGAGAGCCTTGTGCCCGAGTCGAAGCCTAACGTTGCCTCGGCCATTGCAATTGGTACATGTTCTAATCTTTAAGCTATGTTTAGGAGTGCTTTGGCATGACAAAAAAGTACTTCTCACTCACAATCCTCGTATCTAAAAAGTTAGAAGCAATCTAAAGGAAAGTGCTTGTAGAATGGTTTTGTCCCAAGACTTTTAAGAGTATAGAGCTAAATATAAAAGTTATTAGGGCCCGTTTGGTTCAATCCGACTTACGTTTCTTCCTTGAATCAAGATTTGTTTTTTAAAACTGTTTTTCGGATTCCCTGATCCAAACAGTAAAAATTCAAAAAACAACATTTTTATGCTTTCAGAATTTTCAGGTTTTGAATTTTGAAACTTTAAAATGTAGAATTACGTTTGAGGAAAAAAATAATAAAATTTTGATAAATATATTATTTCATGGTATAAACTCATTTCGTTTTTGTAATATTTAGTTGGTAACTACTACTAGTTACAAATATATTGCTGAACACGATGAACAACTTTTTAAATTGATATTCAATTTTCAAATTTACTACCAAACACATATCTGAAAATAAAGAATATAACCTTGTTTTCAGATTTCTATTTTCTGCCATTTAGTCTCTCTTCTGATTCAAAATGGTGTAATGTGTATCCCCTCCTGTTCCAAACATGGAATAGATGAAATAAAAAAAAATAGATTTTTATTCAAGAATTAAATCTTATTGGAACTGTCCATATACAATGTATTATTCGGAACCATATCACATCCCAGATTTGATGAAATAGGATGAATTGAGACGGTATTCTGTAAATACGTAATTATCTTGAATATATTAACTATTTCTTTATTTTACGATGCTCGGAAGAGAAAAAGAAACATCTTGTTCTTTCTTCAACAATTTCCTCTTTTCTGATCACCTACCCAACTCACCCAGTTCTAGTTATTAAACATTTTTGCTCCAAACACACCACATGCCACATTGATAAGTGTATACTGCACAGTTTTGTTGGTGTATTGGAGAACTAAAATTTTTCTCTACATCACAGGGATTGTACTTGGAGTATTTGACGACAACAGATACAGATCAGAGCCCAAAAGGACTGTACTTCAATCTGCGGATATTCTTGGTCTTGGATTTGGACCTGTACTGGACAAAAAGCTGAAATATGCACAAAGTGTCAGTTCTGCGATAGTTTTTGGAAGAGAACTTGTAAATTCACCTGCAAATATACTTACCCCAGGTTCGCTCCTAAATTTTATGTTCTAAAGTTTGTCATTTACTTTCTTGCTAGTGGACTATGGAACCATCAGCATTTTTCAATTTTCCACTGCCATGAAAGGGAAGTTCTTACATTTTTGTAACATGTCATAGCATTTATTTCATGATTGTAACAATACATGGTGTACTGATTTTGCTAAATTACATCCACATGTTAGGGGAATTGGCACTAGAGGCTTTAAAGATTACCTCAGTGCACAGCGATGTTCTTTCTGCAAACATTTACAATGAAGGGGAAATCATAGAATTGAAGATGGGGTCCTATCTTGGCGTTGCTGCAGCAGCCACCGCAAATCCTCCCCACTTTATTCACTTGATTTACAAACCTCCTGGTAGGCCTGCGGCAATCAAATTGGGTTTAGTTGGTAAAGGAATAACCTTTGACAGGTGATTATACACCTACCAAAATGCTTAACTTTCTTTTGCAAATGCTAAACAAAACAAGAATGGACATATATATATATGTTTGAGAGTGTTTTTGACATGGTTAAAAGTACTTATGTCCTGGCTTAGAGCACTTTTCACTCAACCATTATGTCTAGGAGTAACATAAAAGTGTTTTGATAGGATTAGTATTAGGATATCAAGGGTATATTAGTAATTGGATTGGGAATTTGGTTATAAATACAGTAGTAGGTTAAAGATGAAGGTAGGCAATTAGTGAATATGTTTTAAGCTTGAGTAAGAATACTCAAGAGAGGGAGGGTCCAAGTACCTCGAATAACTTGGTAACTATTGTAGTCCTTATTTTTTGTTATATTTCTATTCAGGTTCTATCATGTTTCTGACCCAAAAAACACCTAAAACTAGACTAAGAACCGCCTGAAAAGTTTTTTAAGAGTGTTGAACCAAACATTAAAGTGATTTGTTATTCAACACTTCTATAAAAGTGTTTATAATTAAAGCACTTTTGCTAAAAATACTCCAAGCACACCCTAAGTCAATAAAAGATTTTATTTATAGTATTCATGCAATAGCATATCTCTCATCTTCTGGCTCTGCTGCCGTGATATTTAGTACTTATCTAGTGTTTGATTTATGTTTCCTTTGCACATGCTAGTGATATTATCTGTCTCGTGAGATATGGTTGGTTCTAATTTCAACACACATGGTTGAATAACTCTCTGTTTTCCCCTTTCCATTAGTGGTGGCTACAACCTTAAAACAGGAGCCAACAGTAACATTGAAAATATGAAAAATGATATGGGAGGTGCAGCAGCACTTCTTGGAGCAGCAAAAGCCATTGGTGAAATTAAGCCTCTTGATGTAGAGGTAAACACTAGATTTTTTCACTCCTTTCCATGTCATTAGGAATCTTAATTGTGCTAATAGGTTTAATGGGGGCTTGTTTTCTTGTTTTCTTGTCCCTAAACAGATTCATTTTGTTGTTGCTGCCTGTGAGAACATGATAAGTGCAACTGGCATAAGACCTGGGGATATTGTCACAGCTTCAAATGGAAAGACAATTGAGGTATATCTTCTTTGGTTCATTAACCATCCTGGGCGACTCATTAGTCCAAAGAGACTAGGGTTAATTGAAAGGGAATAAGTTAAAACTCCATAATGGTCACCTACTTTAGGAAGTAATAATTCCTATGTGTTTTCCGGCACCAAATGTTGTAGGGTCAGACGGTTGCCCTATGAGATTAGTCGAGGTGGGTGCAAGCTGGCCTGAACACTCACGGATATCAAAAAGAAAAAATCTTCTTTGGTTCATTGACCCAGAAAAAGGATCTTTGGGTTATTATCAATGATAATTTATCATTGTGAAAATTATGTTTTTGTTGAGTTTGGTCCTAAAATAGTTGTCACTTGGATGACAAGAAGTGTGTGGCTGAGCAGAGTTTCACTGTTATATATCTGATTCCAAGGGGATGGGTATAAAAAGTCTTGCTGCATAAACAAAAATATTCTACTTACAATCTTAATATAAAACTCTTGAATGATATAATAGTAACAGTAACAATGGCAATAACAGTGAGTTTAGATTAAATTGGTAAAATTATTTTTAAAATTTTAGTGGTACTTGATTAGATAATTCAAAGTTTTCAAAAATACTTTAAGAAACACTCTAAAGTGTTTTAGATGAAGCACTTAATTAGTGCCTTTTTTAAATGTATTTTTTTCAAAAGTCATCATTGATAATGAGATCTCGAGAAGCATAAATTGCTCCTGTCAACTTAGCCAACATTGACAGCACTTAATCTATATACCTGCTTTGTGTTGTCAAAGTCTAAGAAGAAAATAGGATTTCATTTTAAACAGAATTTTTCTCTCTTCTTTCCCCTTCCATTACAAATCATAGTAAAGTACATATTCAAATTATTATCTTTCACTGTGGCCAATTTTCAGGTTAACAACACTGATGCTGAAGGCAGACTTTGTCTTGCTGATGCTTTGATATACACTTGTAATCAGGGCGTTGACAAGGTAGATATAGAAATAAGTTAGATGTTTGAAAAGATATGTCATTGCTAATAGTTTTGGGAATTGGGTCTAATGCAACATCATCAAATTGCTCTGCAGATTATTGACCTGGCTACTCTAACTGGTGCTTGTATAACTGCTCTTGGGCCTTCAGTTGCAGGTATGATAATCTCAATTTGAAAAATAAGAACATAAAATTGGGTTTCTTGTGAGTTGTTAATAACATTGGATCAAAGGTCAGGTAGAACATAAGAGTTCTAAATAATGCTACATTGTGTGATCAAAATGTTAATGTTAGAGACACTTTGATTGTGAAACCTTTTCTTTCTTCTCCCTCTCTTGGAATGGTGGGTGGTTTCCTGTCAAAGTTAGAACACAAGAATGAATGAGAAAGAGGACAATTACGTATCGAAACCCAACTGAAAAATTGGAGACTACAGAAGAATGGGATTGTTACAAACTTCATTTTTTGTTTCCAGGTGCCTTTACACCCAATGAAGACCTAGCAAAAGAGGTATTTGCTGCAGCAGAGAGCAGTGGTGAGAAAATATGGAGGCTGCCATTGGAGGAAAGTTATTGGGAATCAATGAAGTCAGGGGTGGCTGATATGATCAACACAGGTCCTGGTCAAGGTGGTGCTATTACAGCTGCTCTGTTTTTGAAACAGGTGGATATCATCTTCTTTGTTCACCTTCTAGAAAAGGAAAAGTTTATGTTCGGTTAAATTACAAGTTCTAGTCCATGAACTTTTTCGGGGTTGAGTTTGTCTGATAGGTCTATGTATTTTCAATTGTGTCTAACAGAATCTGAACTTTAAAAGTGTCCGATAGGTTCCTAAACTTCTAATTTTGTGTCCAATAGGTCCCTAATTAATTAGACATTTTTTAAAACTAATGAACGTATATTAAACAGAAAATTGAAATCTTGTGCCTAATAAATCTGTTAAGTTTTAAAAAATATTGAATAAATCAAGGGTCTATTAGACACAAAATTAAAAGTTTGAAGATCAATGAGACACGAAATTGAAAGTTAAGGAATCTATTAGATACCTTTTAAAGTTCTCGGATTATACTCATAACTTAGTTTAGCCTTTATATTTTTACGAAGGAAATAATGTCTTAAGTAGCTCTAGAAACCTACTATGCCTCTGAATATGAGGTTATCCATTAAAACTCTAAAATTTGGAAATATGTTTCTGTAGTTTGTTGATGAGAAGGTCCAATGGATGCACCTTGACATTGCTGGTCCAGTCTGGAACAGCAAGAAGAGTGTTGCAACAGGATTCGGCATCTCGACACTAGTGGAGTGGGTTCTGAAGAATGCTGCT

mRNA sequence

ATGCTACCTGTTTGTAAAAATGTCTCAAAGATAAAATTTGATGCAAAACGCCTGGATGAGTTGGAATGGAATGGAGACCTACTAGCTGTGGGCGTGACACAGGAAGATGTGGCCAAGGATGAAAACTCCATCTTCGAGAATCCACTTTTGTACAGGTTGGATGCAAGGTTGAATGGGCTGCTGGCTGAAGCTTGTTCAGAGGAAGATTTCTCTGGAAAATCTGGCCAATCTCTTGTTCTAAGAGTTTCTGGTCTTGCCTCTAAGAGGGTTGGTTTATTTGGCCTTGGACAGTCAGCTTCTACTGTTGCAGCCATTCAAGGTCTAGGTGAGGCCATTGCAGCAGCAGCAAAGGCATCTCGAGCAAGTTCAGTTGCTGTATCTCTTGCCTTTTCCGAGAGCCTTGTGCCCGAGTCGAAGCCTAACGTTGCCTCGGCCATTGCAATTGGGATTGTACTTGGAGTATTTGACGACAACAGATACAGATCAGAGCCCAAAAGGACTGTACTTCAATCTGCGGATATTCTTGGTCTTGGATTTGGACCTGTACTGGACAAAAAGCTGAAATATGCACAAAGTGTCAGTTCTGCGATAGTTTTTGGAAGAGAACTTGTAAATTCACCTGCAAATATACTTACCCCAGGGGAATTGGCACTAGAGGCTTTAAAGATTACCTCAGTGCACAGCGATGTTCTTTCTGCAAACATTTACAATGAAGGGGAAATCATAGAATTGAAGATGGGGTCCTATCTTGGCGTTGCTGCAGCAGCCACCGCAAATCCTCCCCACTTTATTCACTTGATTTACAAACCTCCTGGTAGGCCTGCGGCAATCAAATTGGGTTTAGTTGGTAAAGGAATAACCTTTGACAGTGGTGGCTACAACCTTAAAACAGGAGCCAACAGTAACATTGAAAATATGAAAAATGATATGGGAGGTGCAGCAGCACTTCTTGGAGCAGCAAAAGCCATTGGTGAAATTAAGCCTCTTGATGTAGAGATTCATTTTGTTGTTGCTGCCTGTGAGAACATGATAAGTGCAACTGGCATAAGACCTGGGGATATTGTCACAGCTTCAAATGGAAAGACAATTGAGGTTAACAACACTGATGCTGAAGGCAGACTTTGTCTTGCTGATGCTTTGATATACACTTGTAATCAGGGCGTTGACAAGATTATTGACCTGGCTACTCTAACTGGTGCTTGTATAACTGCTCTTGGGCCTTCAGTTGCAGGTGCCTTTACACCCAATGAAGACCTAGCAAAAGAGGTATTTGCTGCAGCAGAGAGCAGTGGTGAGAAAATATGGAGGCTGCCATTGGAGGAAAGTTATTGGGAATCAATGAAGTCAGGGGTGGCTGATATGATCAACACAGGTCCTGGTCAAGGTGGTGCTATTACAGCTGCTCTGTTTTTGAAACAGTTTGTTGATGAGAAGGTCCAATGGATGCACCTTGACATTGCTGGTCCAGTCTGGAACAGCAAGAAGAGTGTTGCAACAGGATTCGGCATCTCGACACTAGTGGAGTGGGTTCTGAAGAATGCTGCT

Coding sequence (CDS)

ATGCTACCTGTTTGTAAAAATGTCTCAAAGATAAAATTTGATGCAAAACGCCTGGATGAGTTGGAATGGAATGGAGACCTACTAGCTGTGGGCGTGACACAGGAAGATGTGGCCAAGGATGAAAACTCCATCTTCGAGAATCCACTTTTGTACAGGTTGGATGCAAGGTTGAATGGGCTGCTGGCTGAAGCTTGTTCAGAGGAAGATTTCTCTGGAAAATCTGGCCAATCTCTTGTTCTAAGAGTTTCTGGTCTTGCCTCTAAGAGGGTTGGTTTATTTGGCCTTGGACAGTCAGCTTCTACTGTTGCAGCCATTCAAGGTCTAGGTGAGGCCATTGCAGCAGCAGCAAAGGCATCTCGAGCAAGTTCAGTTGCTGTATCTCTTGCCTTTTCCGAGAGCCTTGTGCCCGAGTCGAAGCCTAACGTTGCCTCGGCCATTGCAATTGGGATTGTACTTGGAGTATTTGACGACAACAGATACAGATCAGAGCCCAAAAGGACTGTACTTCAATCTGCGGATATTCTTGGTCTTGGATTTGGACCTGTACTGGACAAAAAGCTGAAATATGCACAAAGTGTCAGTTCTGCGATAGTTTTTGGAAGAGAACTTGTAAATTCACCTGCAAATATACTTACCCCAGGGGAATTGGCACTAGAGGCTTTAAAGATTACCTCAGTGCACAGCGATGTTCTTTCTGCAAACATTTACAATGAAGGGGAAATCATAGAATTGAAGATGGGGTCCTATCTTGGCGTTGCTGCAGCAGCCACCGCAAATCCTCCCCACTTTATTCACTTGATTTACAAACCTCCTGGTAGGCCTGCGGCAATCAAATTGGGTTTAGTTGGTAAAGGAATAACCTTTGACAGTGGTGGCTACAACCTTAAAACAGGAGCCAACAGTAACATTGAAAATATGAAAAATGATATGGGAGGTGCAGCAGCACTTCTTGGAGCAGCAAAAGCCATTGGTGAAATTAAGCCTCTTGATGTAGAGATTCATTTTGTTGTTGCTGCCTGTGAGAACATGATAAGTGCAACTGGCATAAGACCTGGGGATATTGTCACAGCTTCAAATGGAAAGACAATTGAGGTTAACAACACTGATGCTGAAGGCAGACTTTGTCTTGCTGATGCTTTGATATACACTTGTAATCAGGGCGTTGACAAGATTATTGACCTGGCTACTCTAACTGGTGCTTGTATAACTGCTCTTGGGCCTTCAGTTGCAGGTGCCTTTACACCCAATGAAGACCTAGCAAAAGAGGTATTTGCTGCAGCAGAGAGCAGTGGTGAGAAAATATGGAGGCTGCCATTGGAGGAAAGTTATTGGGAATCAATGAAGTCAGGGGTGGCTGATATGATCAACACAGGTCCTGGTCAAGGTGGTGCTATTACAGCTGCTCTGTTTTTGAAACAGTTTGTTGATGAGAAGGTCCAATGGATGCACCTTGACATTGCTGGTCCAGTCTGGAACAGCAAGAAGAGTGTTGCAACAGGATTCGGCATCTCGACACTAGTGGAGTGGGTTCTGAAGAATGCTGCT

Protein sequence

MLPVCKNVSKIKFDAKRLDELEWNGDLLAVGVTQEDVAKDENSIFENPLLYRLDARLNGLLAEACSEEDFSGKSGQSLVLRVSGLASKRVGLFGLGQSASTVAAIQGLGEAIAAAAKASRASSVAVSLAFSESLVPESKPNVASAIAIGIVLGVFDDNRYRSEPKRTVLQSADILGLGFGPVLDKKLKYAQSVSSAIVFGRELVNSPANILTPGELALEALKITSVHSDVLSANIYNEGEIIELKMGSYLGVAAAATANPPHFIHLIYKPPGRPAAIKLGLVGKGITFDSGGYNLKTGANSNIENMKNDMGGAAALLGAAKAIGEIKPLDVEIHFVVAACENMISATGIRPGDIVTASNGKTIEVNNTDAEGRLCLADALIYTCNQGVDKIIDLATLTGACITALGPSVAGAFTPNEDLAKEVFAAAESSGEKIWRLPLEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDEKVQWMHLDIAGPVWNSKKSVATGFGISTLVEWVLKNAA
Homology
BLAST of MS007215 vs. NCBI nr
Match: XP_022158161.1 (leucine aminopeptidase 2, chloroplastic-like [Momordica charantia])

HSP 1 Score: 975.7 bits (2521), Expect = 1.5e-280
Identity = 504/513 (98.25%), Postives = 507/513 (98.83%), Query Frame = 0

Query: 3   PVCKNVSKIKFDAKRLDELEWNGDLLAVGVTQEDVAKDENSIFENPLLYRLDARLNGLLA 62
           P   N  KIKFDAK LDELEWNGDLLAVGVTQEDVAKDENSIFENPLLYRLDARLNGLLA
Sbjct: 16  PGPSNAPKIKFDAKHLDELEWNGDLLAVGVTQEDVAKDENSIFENPLLYRLDARLNGLLA 75

Query: 63  EACSEEDFSGKSGQSLVLRVSGLASKRVGLFGLGQSASTVAAIQGLGEAIAAAAKASRAS 122
           EAC+EEDFSGKSGQSLVLRVSGLASKRVGLFGLGQSASTVAAIQGLGEAIAAAAKASRAS
Sbjct: 76  EACAEEDFSGKSGQSLVLRVSGLASKRVGLFGLGQSASTVAAIQGLGEAIAAAAKASRAS 135

Query: 123 SVAVSLAFSESLVPESKPNVASAIAIGIVLGVFDDNRYRSEPKRTVLQSADILGLGFGPV 182
           SVAVSLAFSESLVPESKPNVASAIAIGIVLGVFDDNRYRSEPKRTVLQSADILGLGFGPV
Sbjct: 136 SVAVSLAFSESLVPESKPNVASAIAIGIVLGVFDDNRYRSEPKRTVLQSADILGLGFGPV 195

Query: 183 LDKKLKYAQSVSSAIVFGRELVNSPANILTPGELALEALKITSVHSDVLSANIYNEGEII 242
           LDKKLKYAQSVSSAIVFGRELVNSPANILTPGELALEALKITSVHSDVLSANIYNEGEII
Sbjct: 196 LDKKLKYAQSVSSAIVFGRELVNSPANILTPGELALEALKITSVHSDVLSANIYNEGEII 255

Query: 243 ELKMGSYLGVAAAATANPPHFIHLIYKPPGRPAAIKLGLVGKGITFDSGGYNLKTGANSN 302
           ELKMGSYLG+AAAATANPPHFIHLIYKPPGRPAAIKLGLVGKGITFDSGGYNLKTGANSN
Sbjct: 256 ELKMGSYLGIAAAATANPPHFIHLIYKPPGRPAAIKLGLVGKGITFDSGGYNLKTGANSN 315

Query: 303 IENMKNDMGGAAALLGAAKAIGEIKPLDVEIHFVVAACENMISATGIRPGDIVTASNGKT 362
           IENMKNDMGGAAALLGAAKAIGEIKPLDVEIHFVVAACENMISATG+RPGDIVTASNGKT
Sbjct: 316 IENMKNDMGGAAALLGAAKAIGEIKPLDVEIHFVVAACENMISATGMRPGDIVTASNGKT 375

Query: 363 IEVNNTDAEGRLCLADALIYTCNQGVDKIIDLATLTGACITALGPSVAGAFTPNEDLAKE 422
           IEVNNTDAEGRLCLADALIYTCNQGVDKIIDLATLTGACITALGPSVAGAFTPNEDLAKE
Sbjct: 376 IEVNNTDAEGRLCLADALIYTCNQGVDKIIDLATLTGACITALGPSVAGAFTPNEDLAKE 435

Query: 423 VFAAAESSGEKIWRLPLEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDEKVQWM 482
           VFAAAESSGEKIWRLPLEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDEKVQWM
Sbjct: 436 VFAAAESSGEKIWRLPLEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDEKVQWM 495

Query: 483 HLDIAGPVWNSKKSVATGFGISTLVEWVLKNAA 516
           HLDIAGPVWNSKKSVATGFGISTLVEWVLKNAA
Sbjct: 496 HLDIAGPVWNSKKSVATGFGISTLVEWVLKNAA 528

BLAST of MS007215 vs. NCBI nr
Match: XP_022999678.1 (leucine aminopeptidase 2, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 814.7 bits (2103), Expect = 4.6e-232
Identity = 417/513 (81.29%), Postives = 453/513 (88.30%), Query Frame = 0

Query: 3   PVCKNVSKIKFDAKRLDELEWNGDLLAVGVTQEDVAKDENSIFENPLLYRLDARLNGLLA 62
           P   N  KI F AK LD LEWNGDL+AVGV ++DV KDE   F+NPLLY+L+  L GLLA
Sbjct: 14  PGPSNAPKISFVAKDLDVLEWNGDLVAVGVREKDVEKDEAGHFKNPLLYQLNEALGGLLA 73

Query: 63  EACSEEDFSGKSGQSLVLRVSGLASKRVGLFGLGQSASTVAAIQGLGEAIAAAAKASRAS 122
           EA SEEDFSGKS QSLVLRVSGL  KRVGLFGLGQSAS+ AA  GLGEAIAAAA+ASRA+
Sbjct: 74  EASSEEDFSGKSAQSLVLRVSGLRFKRVGLFGLGQSASSAAAFVGLGEAIAAAAQASRAA 133

Query: 123 SVAVSLAFSESLVPESKPNVASAIAIGIVLGVFDDNRYRSEPKRTVLQSADILGLGFGPV 182
           S+A+SLAFSE L  ESKP +ASAIA+GIV G+FDDNRY+S PK T+LQS D+LGLGFGP 
Sbjct: 134 SLALSLAFSEDLSAESKPGIASAIALGIVNGIFDDNRYKSNPKMTLLQSVDVLGLGFGPN 193

Query: 183 LDKKLKYAQSVSSAIVFGRELVNSPANILTPGELALEALKITSVHSDVLSANIYNEGEII 242
           ++KKLKYA+ VSS +VFG+ELVNSPANILTPG+LA E LKIT  + DVLSA I+NE EII
Sbjct: 194 MEKKLKYAEYVSSGVVFGKELVNSPANILTPGQLAGEVLKITHKYHDVLSAKIFNEEEII 253

Query: 243 ELKMGSYLGVAAAATANPPHFIHLIYKPPGRPAAIKLGLVGKGITFDSGGYNLKTGANSN 302
           E+KMGSYLGV AAATANPPHFIHL YKPPG   + KLGLVGKGITFDSGGYNLKTGANSN
Sbjct: 254 EMKMGSYLGVTAAATANPPHFIHLCYKPPGGSVSTKLGLVGKGITFDSGGYNLKTGANSN 313

Query: 303 IENMKNDMGGAAALLGAAKAIGEIKPLDVEIHFVVAACENMISATGIRPGDIVTASNGKT 362
           IE MKNDMGGAAA+ GAAKAI E+KP  VEIHFVVAACENMISATG+RP DIVTASNGKT
Sbjct: 314 IETMKNDMGGAAAIFGAAKAIAELKPPGVEIHFVVAACENMISATGMRPSDIVTASNGKT 373

Query: 363 IEVNNTDAEGRLCLADALIYTCNQGVDKIIDLATLTGACITALGPSVAGAFTPNEDLAKE 422
           IEVNNTDAEGRLCLADALIYTCN GV+KIIDLATLTGACITALGPSVAGAFTPNE+LAKE
Sbjct: 374 IEVNNTDAEGRLCLADALIYTCNLGVEKIIDLATLTGACITALGPSVAGAFTPNEELAKE 433

Query: 423 VFAAAESSGEKIWRLPLEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDEKVQWM 482
           V  AAE SGEKIWRLP+EESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDE VQWM
Sbjct: 434 VIDAAERSGEKIWRLPMEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDENVQWM 493

Query: 483 HLDIAGPVWNSKKSVATGFGISTLVEWVLKNAA 516
           HLDIAGPVWN+KKSVATGFG+STLVEWVLKN +
Sbjct: 494 HLDIAGPVWNTKKSVATGFGVSTLVEWVLKNVS 526

BLAST of MS007215 vs. NCBI nr
Match: XP_022946689.1 (leucine aminopeptidase 2, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 811.6 bits (2095), Expect = 3.9e-231
Identity = 416/512 (81.25%), Postives = 451/512 (88.09%), Query Frame = 0

Query: 3   PVCKNVSKIKFDAKRLDELEWNGDLLAVGVTQEDVAKDENSIFENPLLYRLDARLNGLLA 62
           P   N  KI F AK LD LEWNGDL+AVGV ++DV KDE   F+NPLLY+LD  L GLL 
Sbjct: 14  PGPSNAPKITFVAKDLDVLEWNGDLVAVGVREKDVEKDEAGHFKNPLLYQLDEALGGLLG 73

Query: 63  EACSEEDFSGKSGQSLVLRVSGLASKRVGLFGLGQSASTVAAIQGLGEAIAAAAKASRAS 122
           EA SEEDFSGKS QSLVLRVSGL  KRVGLFGLGQSAS+ AA  GLGEAIAAAA+ SRA+
Sbjct: 74  EASSEEDFSGKSAQSLVLRVSGLKFKRVGLFGLGQSASSAAAFVGLGEAIAAAAQTSRAA 133

Query: 123 SVAVSLAFSESLVPESKPNVASAIAIGIVLGVFDDNRYRSEPKRTVLQSADILGLGFGPV 182
           S+A++LAFSE L  ESKP +ASAIA+GIV G+FDDNRY+S PK TVLQ  D+LGLGFGP 
Sbjct: 134 SLALALAFSEDLSAESKPGIASAIALGIVNGIFDDNRYKSNPKMTVLQFVDVLGLGFGPN 193

Query: 183 LDKKLKYAQSVSSAIVFGRELVNSPANILTPGELALEALKITSVHSDVLSANIYNEGEII 242
           ++KKLKYA+ VSS +VFG+ELVNSPANILTPG+LA E LKIT  ++DVLSA I+NE EII
Sbjct: 194 MEKKLKYAEYVSSGVVFGKELVNSPANILTPGQLAGEVLKITHKYNDVLSAKIFNEEEII 253

Query: 243 ELKMGSYLGVAAAATANPPHFIHLIYKPPGRPAAIKLGLVGKGITFDSGGYNLKTGANSN 302
           E+KMGSYLGV AAATANPPHFIHL YKP G   + KLGLVGKGITFDSGGYNLKTGANSN
Sbjct: 254 EMKMGSYLGVTAAATANPPHFIHLCYKPLGGSVSTKLGLVGKGITFDSGGYNLKTGANSN 313

Query: 303 IENMKNDMGGAAALLGAAKAIGEIKPLDVEIHFVVAACENMISATGIRPGDIVTASNGKT 362
           IE MKNDMGGAAA+ GAAKAI E+KP  VEIHFVVAACENMISATG+RP DIVTASNGKT
Sbjct: 314 IETMKNDMGGAAAIFGAAKAIAELKPPGVEIHFVVAACENMISATGMRPSDIVTASNGKT 373

Query: 363 IEVNNTDAEGRLCLADALIYTCNQGVDKIIDLATLTGACITALGPSVAGAFTPNEDLAKE 422
           IEVNNTDAEGRLCLADALIYTCN GV+KIIDLATLTGACITALGPSVAGAFTPNE+LAKE
Sbjct: 374 IEVNNTDAEGRLCLADALIYTCNLGVEKIIDLATLTGACITALGPSVAGAFTPNEELAKE 433

Query: 423 VFAAAESSGEKIWRLPLEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDEKVQWM 482
           V AAAE SGEKIWRLP+EESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDE VQWM
Sbjct: 434 VLAAAERSGEKIWRLPMEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDENVQWM 493

Query: 483 HLDIAGPVWNSKKSVATGFGISTLVEWVLKNA 515
           HLDIAGPVWN+KKSVATGFG+STLVEWVLKNA
Sbjct: 494 HLDIAGPVWNTKKSVATGFGVSTLVEWVLKNA 525

BLAST of MS007215 vs. NCBI nr
Match: KAG6599158.1 (Leucine aminopeptidase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 809.7 bits (2090), Expect = 1.5e-230
Identity = 415/513 (80.90%), Postives = 451/513 (87.91%), Query Frame = 0

Query: 3   PVCKNVSKIKFDAKRLDELEWNGDLLAVGVTQEDVAKDENSIFENPLLYRLDARLNGLLA 62
           P   N  KI F AK LD LEWNGDL+AVGV ++DV KDE   F+NPLLY+LD  L GLL 
Sbjct: 14  PGPSNAPKITFVAKDLDVLEWNGDLVAVGVREKDVEKDEAGHFKNPLLYQLDEALGGLLG 73

Query: 63  EACSEEDFSGKSGQSLVLRVSGLASKRVGLFGLGQSASTVAAIQGLGEAIAAAAKASRAS 122
           EA SEEDFSGKS QSLVLRVSGL  KRVGLFGLGQSA + A   GLGEAIAAAA+ASRA+
Sbjct: 74  EASSEEDFSGKSAQSLVLRVSGLKFKRVGLFGLGQSACSAATFVGLGEAIAAAAQASRAA 133

Query: 123 SVAVSLAFSESLVPESKPNVASAIAIGIVLGVFDDNRYRSEPKRTVLQSADILGLGFGPV 182
           S+A+SLAFSE L  ESKP +ASAIA+GIV G+FDDNRY+S PK TVLQ  D+LGLGFGP 
Sbjct: 134 SLALSLAFSEDLSAESKPGIASAIALGIVNGIFDDNRYKSNPKMTVLQLVDVLGLGFGPN 193

Query: 183 LDKKLKYAQSVSSAIVFGRELVNSPANILTPGELALEALKITSVHSDVLSANIYNEGEII 242
           ++KKLKYA+ VSS +VFG+ELVNSPANILTPG+LA E LKIT  ++DVLSA I+NE EII
Sbjct: 194 MEKKLKYAEYVSSGVVFGKELVNSPANILTPGQLAGEVLKITHKYNDVLSAKIFNEEEII 253

Query: 243 ELKMGSYLGVAAAATANPPHFIHLIYKPPGRPAAIKLGLVGKGITFDSGGYNLKTGANSN 302
           E+KMGSYLGV AAATANPPHFIHL YKP G   + KLGLVGKGITFDSGGYNLKTGANSN
Sbjct: 254 EMKMGSYLGVTAAATANPPHFIHLCYKPLGGSVSTKLGLVGKGITFDSGGYNLKTGANSN 313

Query: 303 IENMKNDMGGAAALLGAAKAIGEIKPLDVEIHFVVAACENMISATGIRPGDIVTASNGKT 362
           IE MKNDMGGAAA+ GAAKAI E+KP  VEIHFVVAACENMISATG+RP DIVTASNGKT
Sbjct: 314 IETMKNDMGGAAAIFGAAKAIAELKPPGVEIHFVVAACENMISATGMRPSDIVTASNGKT 373

Query: 363 IEVNNTDAEGRLCLADALIYTCNQGVDKIIDLATLTGACITALGPSVAGAFTPNEDLAKE 422
           IEVNNTDAEGRLCLADALIYTCN GV+KIIDLATLTGACITALGPSVAGAFTPNE+LAKE
Sbjct: 374 IEVNNTDAEGRLCLADALIYTCNLGVEKIIDLATLTGACITALGPSVAGAFTPNEELAKE 433

Query: 423 VFAAAESSGEKIWRLPLEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDEKVQWM 482
           V AAAE SGEKIWRLP+EESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDE VQWM
Sbjct: 434 VLAAAERSGEKIWRLPMEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDENVQWM 493

Query: 483 HLDIAGPVWNSKKSVATGFGISTLVEWVLKNAA 516
           HLDIAGPVWN+KKSVATGFG+STLVEWVLKN++
Sbjct: 494 HLDIAGPVWNTKKSVATGFGVSTLVEWVLKNSS 526

BLAST of MS007215 vs. NCBI nr
Match: XP_023545271.1 (leucine aminopeptidase 2, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 807.4 bits (2084), Expect = 7.3e-230
Identity = 414/513 (80.70%), Postives = 451/513 (87.91%), Query Frame = 0

Query: 3   PVCKNVSKIKFDAKRLDELEWNGDLLAVGVTQEDVAKDENSIFENPLLYRLDARLNGLLA 62
           P   N  KI F AK LD LEWNGDL+AVGV ++DV KDE   F+NPLLY+L+  L GLL 
Sbjct: 14  PGPSNAPKIIFVAKDLDVLEWNGDLVAVGVREKDVEKDEAGHFKNPLLYQLNEALGGLLG 73

Query: 63  EACSEEDFSGKSGQSLVLRVSGLASKRVGLFGLGQSASTVAAIQGLGEAIAAAAKASRAS 122
           EA SEEDFSGKS QSLVLRVSGL  KRVGLFGLGQSAS+ AA  GLGEAIAAAA+ASRA+
Sbjct: 74  EASSEEDFSGKSAQSLVLRVSGLTFKRVGLFGLGQSASSAAAFVGLGEAIAAAAQASRAA 133

Query: 123 SVAVSLAFSESLVPESKPNVASAIAIGIVLGVFDDNRYRSEPKRTVLQSADILGLGFGPV 182
           S+A++LAFSE L  ESKP +ASAIA+GIV G+FDDNRY+S PK ++LQS D+LGLGFGP 
Sbjct: 134 SLALALAFSEDLSAESKPGIASAIALGIVNGIFDDNRYKSNPKMSLLQSVDVLGLGFGPN 193

Query: 183 LDKKLKYAQSVSSAIVFGRELVNSPANILTPGELALEALKITSVHSDVLSANIYNEGEII 242
           ++KKLKYA+ VSS IVFG+ELVNSPANILTPGELA E  KI   ++DVLSANI+ E +II
Sbjct: 194 MEKKLKYAEYVSSGIVFGKELVNSPANILTPGELAEEVSKIAEKYNDVLSANIFKEEKII 253

Query: 243 ELKMGSYLGVAAAATANPPHFIHLIYKPPGRPAAIKLGLVGKGITFDSGGYNLKTGANSN 302
           ELKMGSYLGV AAATANPPHFIHL YKPPG   + KLGLVGKGITFDSGGYNLK GANSN
Sbjct: 254 ELKMGSYLGVTAAATANPPHFIHLCYKPPGGSVSTKLGLVGKGITFDSGGYNLKAGANSN 313

Query: 303 IENMKNDMGGAAALLGAAKAIGEIKPLDVEIHFVVAACENMISATGIRPGDIVTASNGKT 362
           IE MKNDMGGAAA+ GAAKAI E+KP  VEIHFVVAACENMISATG+RP DIVTASNGKT
Sbjct: 314 IETMKNDMGGAAAIFGAAKAIAELKPPGVEIHFVVAACENMISATGMRPSDIVTASNGKT 373

Query: 363 IEVNNTDAEGRLCLADALIYTCNQGVDKIIDLATLTGACITALGPSVAGAFTPNEDLAKE 422
           IEVNNTDAEGRLCLADALIYTC  GV+KIIDLATLTGACITALGPSVAGAFTPNE+LAKE
Sbjct: 374 IEVNNTDAEGRLCLADALIYTCKLGVEKIIDLATLTGACITALGPSVAGAFTPNEELAKE 433

Query: 423 VFAAAESSGEKIWRLPLEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDEKVQWM 482
           V AAAE SGEKIWRLP+EESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDE VQWM
Sbjct: 434 VIAAAERSGEKIWRLPMEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDENVQWM 493

Query: 483 HLDIAGPVWNSKKSVATGFGISTLVEWVLKNAA 516
           HLDIAGPVWN+KKSVATGFG+STLVEWVLKNA+
Sbjct: 494 HLDIAGPVWNTKKSVATGFGVSTLVEWVLKNAS 526

BLAST of MS007215 vs. ExPASy Swiss-Prot
Match: Q944P7 (Leucine aminopeptidase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LAP2 PE=2 SV=2)

HSP 1 Score: 699.9 bits (1805), Expect = 2.1e-200
Identity = 354/506 (69.96%), Postives = 419/506 (82.81%), Query Frame = 0

Query: 10  KIKFDAKRLDELEWNGDLLAVGVTQEDVAKDENSIFENPLLYRLDARLNGLLAEACSEED 69
           KI F  K +D  EW GD+LAVGVT++D+AKD NS FENP+L +LDA L GLLA+  SEED
Sbjct: 78  KISFSGKEIDVTEWKGDILAVGVTEKDMAKDVNSKFENPILKKLDAHLGGLLADVSSEED 137

Query: 70  FSGKSGQSLVLRVSGLASKRVGLFGLGQSASTVAAIQGLGEAIAAAAKASRASSVAVSLA 129
           FSGK GQS VLR+ GL SKRVGL GLG+SAST +A Q LGEA+AAAAKAS+ASSVAV LA
Sbjct: 138 FSGKPGQSTVLRLPGLGSKRVGLIGLGKSASTPSAFQSLGEAVAAAAKASQASSVAVVLA 197

Query: 130 FSESLVPESKPNVASAIAIGIVLGVFDDNRYRSEPKRTVLQSADILGLGFGPVLDKKLKY 189
            SES+  ESK   ASAIA G VLG+F+D+RY+SE K+  L+S DI+G G GP L+KKLKY
Sbjct: 198 SSESVSNESKLCSASAIASGTVLGLFEDSRYKSESKKPSLKSVDIIGFGSGPELEKKLKY 257

Query: 190 AQSVSSAIVFGRELVNSPANILTPGELALEALKITSVHSDVLSANIYNEGEIIELKMGSY 249
           A+ VS  ++FG+ELVNSPAN+LTP  LA EAL + S++SDV++ANI NE +  ELKMGSY
Sbjct: 258 AEHVSYGVIFGKELVNSPANVLTPAVLAEEALNLASMYSDVMTANILNEEQCKELKMGSY 317

Query: 250 LGVAAAATANPPHFIHLIYKPPGRPAAIKLGLVGKGITFDSGGYNLKTGANSNIENMKND 309
           L V AAA+ANPPHFIHLIYKP   P   KL LVGKG+TFDSGGYN+KTG    IE MK D
Sbjct: 318 LAV-AAASANPPHFIHLIYKPSSGPVKTKLALVGKGLTFDSGGYNIKTGPGCLIELMKFD 377

Query: 310 MGGAAALLGAAKAIGEIKPLDVEIHFVVAACENMISATGIRPGDIVTASNGKTIEVNNTD 369
           MGG+AA+LGAAKAIG+IKP  VE+HF+VAACENMIS TG+RPGD++TASNGKTIEVNNTD
Sbjct: 378 MGGSAAVLGAAKAIGQIKPPGVEVHFIVAACENMISGTGMRPGDVLTASNGKTIEVNNTD 437

Query: 370 AEGRLCLADALIYTCNQGVDKIIDLATLTGACITALGPSVAGAFTPNEDLAKEVFAAAES 429
           AEGRL LADAL+Y CNQGVDK++DLATLTGACI ALG S+AG +TP++ LAKEV AA+E 
Sbjct: 438 AEGRLTLADALVYACNQGVDKVVDLATLTGACIIALGTSMAGIYTPSDKLAKEVIAASER 497

Query: 430 SGEKIWRLPLEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDEKVQWMHLDIAGP 489
           SGEK+WR+P+EESYWE MKSGVADM+NTG   GG+ITAALFLKQFV E V+WMH+D+AGP
Sbjct: 498 SGEKLWRMPMEESYWEMMKSGVADMVNTGGRAGGSITAALFLKQFVSEDVEWMHIDMAGP 557

Query: 490 VWNSKKSVATGFGISTLVEWVLKNAA 516
           VWN KK  ATGFG++TLVEWV  +++
Sbjct: 558 VWNEKKKAATGFGVATLVEWVQNHSS 582

BLAST of MS007215 vs. ExPASy Swiss-Prot
Match: P31427 (Leucine aminopeptidase, chloroplastic OS=Solanum tuberosum OX=4113 GN=LAP PE=2 SV=2)

HSP 1 Score: 691.0 bits (1782), Expect = 1.0e-197
Identity = 348/505 (68.91%), Postives = 417/505 (82.57%), Query Frame = 0

Query: 10  KIKFDAKRLDELEWNGDLLAVGVTQEDVAKDENSIFENPLLYRLDARLNGLLAEACSEED 69
           KI   AK  D ++W GDLLA+G T+ D+A+D+NS F+NPLL RLD++LNGLL+ A SEED
Sbjct: 71  KISIGAKDTDVVQWQGDLLAIGATENDLARDDNSKFKNPLLQRLDSKLNGLLSAASSEED 130

Query: 70  FSGKSGQSLVLRVSGLASKRVGLFGLGQSASTVAAIQGLGEAIAAAAKASRASSVAVSLA 129
           FSGKSGQS+ LR+ G    R+ L GLG SAS+  +   LGEA AAAAK+++A ++AVSLA
Sbjct: 131 FSGKSGQSINLRLPG---GRITLVGLGSSASSPTSYHSLGEAAAAAAKSAQARNIAVSLA 190

Query: 130 FSESLVPESKPNVASAIAIGIVLGVFDDNRYRSEPKRTVLQSADILGLGFGPVLDKKLKY 189
            ++ L  ESK N ASAIA G++LG+F+DNR+RSE K   L+S DILGLG GP ++ K+KY
Sbjct: 191 STDGLSAESKINSASAIATGVMLGIFEDNRFRSESKTPALESLDILGLGTGPEIESKIKY 250

Query: 190 AQSVSSAIVFGRELVNSPANILTPGELALEALKITSVHSDVLSANIYNEGEIIELKMGSY 249
           A+ V + ++ GRELVN+PANI+TPG LA EA KI S +SDV++ NI +  +  ELKMG+Y
Sbjct: 251 AEHVCAGVILGRELVNAPANIVTPGALAEEAKKIASTYSDVITVNILDAEQCKELKMGAY 310

Query: 250 LGVAAAATANPPHFIHLIYKPPGRPAAIKLGLVGKGITFDSGGYNLKTGANSNIENMKND 309
           LGVAAAAT NPP+FIHL +K   R    K+ LVGKG+TFDSGGYNLKTGA S IE MKND
Sbjct: 311 LGVAAAATENPPYFIHLCFKTNSRERKTKIALVGKGLTFDSGGYNLKTGAGSKIELMKND 370

Query: 310 MGGAAALLGAAKAIGEIKPLDVEIHFVVAACENMISATGIRPGDIVTASNGKTIEVNNTD 369
           MGGAAA+LGAAKA+GEIKP  VE+HF+VAACENMIS  G+RPGDIVTASNGKTIEVNNTD
Sbjct: 371 MGGAAAVLGAAKALGEIKPRGVEVHFIVAACENMISGAGMRPGDIVTASNGKTIEVNNTD 430

Query: 370 AEGRLCLADALIYTCNQGVDKIIDLATLTGACITALGPSVAGAFTPNEDLAKEVFAAAES 429
           AEGRL LADALIY CNQGV+KIIDLATLTGA +TALGPSVAGAFTP++ LA+EV  AAE+
Sbjct: 431 AEGRLTLADALIYACNQGVEKIIDLATLTGAIVTALGPSVAGAFTPSDGLAREVVVAAEA 490

Query: 430 SGEKIWRLPLEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDEKVQWMHLDIAGP 489
           SGEK+WR+P+EESYWESMKSGVADMINTGP  GGAIT ALFLKQFVDEKVQW+HLDIAGP
Sbjct: 491 SGEKLWRMPMEESYWESMKSGVADMINTGPRDGGAITGALFLKQFVDEKVQWLHLDIAGP 550

Query: 490 VWNSKKSVATGFGISTLVEWVLKNA 515
           VW+ +K  ATG+G+STLVEWVL+N+
Sbjct: 551 VWSDEKKNATGYGVSTLVEWVLRNS 572

BLAST of MS007215 vs. ExPASy Swiss-Prot
Match: P30184 (Leucine aminopeptidase 1 OS=Arabidopsis thaliana OX=3702 GN=LAP1 PE=1 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 3.6e-195
Identity = 340/506 (67.19%), Postives = 412/506 (81.42%), Query Frame = 0

Query: 10  KIKFDAKRLDELEWNGDLLAVGVTQEDVAKDENSIFENPLLYRLDARLNGLLAEACSEED 69
           KI F AK +D +EW GD+L VGVT++D+AKD NS FENP+L ++DA L+GLLA+  SEED
Sbjct: 17  KISFTAKEIDVIEWKGDILVVGVTEKDLAKDGNSKFENPILSKVDAHLSGLLAQVSSEED 76

Query: 70  FSGKSGQSLVLRVSGLASKRVGLFGLGQSASTVAAIQGLGEAIAAAAKASRASSVAVSLA 129
           F+GK GQS VLR+ GL SKR+ L GLGQS S+  A   LGEA+A  +KAS+++S A+ LA
Sbjct: 77  FTGKPGQSTVLRLPGLGSKRIALIGLGQSVSSPVAFHSLGEAVATVSKASQSTSAAIVLA 136

Query: 130 FSESLVPESKPNVASAIAIGIVLGVFDDNRYRSEPKRTVLQSADILGLGFGPVLDKKLKY 189
            S S   ESK +  SA+A GIVLG+F+D RY+SE K+  L++ DI+G G G  ++KKLKY
Sbjct: 137 SSVS--DESKLSSVSALASGIVLGLFEDGRYKSESKKPSLKAVDIIGFGTGAEVEKKLKY 196

Query: 190 AQSVSSAIVFGRELVNSPANILTPGELALEALKITSVHSDVLSANIYNEGEIIELKMGSY 249
           A+ VS  ++FGREL+NSPAN+LTP  LA EA K+ S +SDV +ANI NE +  ELKMGSY
Sbjct: 197 AEDVSYGVIFGRELINSPANVLTPAVLAEEAAKVASTYSDVFTANILNEEQCKELKMGSY 256

Query: 250 LGVAAAATANPPHFIHLIYKPPGRPAAIKLGLVGKGITFDSGGYNLKTGANSNIENMKND 309
           L V AAA+ANPPHFIHL+YKPP      KL LVGKG+TFDSGGYN+KTG   +IE MK D
Sbjct: 257 LAV-AAASANPPHFIHLVYKPPNGSVKTKLALVGKGLTFDSGGYNIKTGPGCSIELMKFD 316

Query: 310 MGGAAALLGAAKAIGEIKPLDVEIHFVVAACENMISATGIRPGDIVTASNGKTIEVNNTD 369
           MGG+AA+LGAAKAIGEIKP  VE+HF+VAACENMIS TG+RPGD++TASNGKTIEVNNTD
Sbjct: 317 MGGSAAVLGAAKAIGEIKPPGVEVHFIVAACENMISGTGMRPGDVITASNGKTIEVNNTD 376

Query: 370 AEGRLCLADALIYTCNQGVDKIIDLATLTGACITALGPSVAGAFTPNEDLAKEVFAAAES 429
           AEGRL LADAL+Y CNQGVDKI+DLATLTGAC+ ALG S+AG +TP+++LAKEV AA+E 
Sbjct: 377 AEGRLTLADALVYACNQGVDKIVDLATLTGACVIALGTSMAGIYTPSDELAKEVIAASER 436

Query: 430 SGEKIWRLPLEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDEKVQWMHLDIAGP 489
           SGEK+WR+PLEESYWE MKSGVADM+NTG   GG+ITAALFLKQFV EKVQWMH+D+AGP
Sbjct: 437 SGEKLWRMPLEESYWEMMKSGVADMVNTGGRAGGSITAALFLKQFVSEKVQWMHIDMAGP 496

Query: 490 VWNSKKSVATGFGISTLVEWVLKNAA 516
           VWN KK   TGFG++TLVEWV KN++
Sbjct: 497 VWNEKKKSGTGFGVATLVEWVQKNSS 519

BLAST of MS007215 vs. ExPASy Swiss-Prot
Match: Q10712 (Leucine aminopeptidase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=LAPA1 PE=1 SV=2)

HSP 1 Score: 682.6 bits (1760), Expect = 3.6e-195
Identity = 345/504 (68.45%), Postives = 413/504 (81.94%), Query Frame = 0

Query: 10  KIKFDAKRLDELEWNGDLLAVGVTQEDVAKDENSIFENPLLYRLDARLNGLLAEACSEED 69
           KI   AK    ++W GDLLA+G T+ D+A+DENS F+NPLL +LD+ LNGLL+ A SEED
Sbjct: 71  KISIGAKDTAVVQWQGDLLAIGATENDMARDENSKFKNPLLQQLDSELNGLLSAASSEED 130

Query: 70  FSGKSGQSLVLRVSGLASKRVGLFGLGQSASTVAAIQGLGEAIAAAAKASRASSVAVSLA 129
           FSGKSGQS+ LR  G    R+ L GLG SAS+  +   LG+A AAAAK+S+A ++AV+LA
Sbjct: 131 FSGKSGQSVNLRFPG---GRITLVGLGSSASSPTSYHSLGQAAAAAAKSSQARNIAVALA 190

Query: 130 FSESLVPESKPNVASAIAIGIVLGVFDDNRYRSEPKRTVLQSADILGLGFGPVLDKKLKY 189
            ++ L  ESK N ASAIA G+VLG F+DNR+RSE K++ L+S DILGLG GP +++K+KY
Sbjct: 191 STDGLSAESKINSASAIATGVVLGSFEDNRFRSESKKSTLESLDILGLGTGPEIERKIKY 250

Query: 190 AQSVSSAIVFGRELVNSPANILTPGELALEALKITSVHSDVLSANIYNEGEIIELKMGSY 249
           A+ V + ++ GRELVN+PANI+TP  LA EA KI S +SDV+S NI +  +  ELKMG+Y
Sbjct: 251 AEHVCAGVILGRELVNAPANIVTPAVLAEEAKKIASTYSDVISVNILDAEQCKELKMGAY 310

Query: 250 LGVAAAATANPPHFIHLIYKPPGRPAAIKLGLVGKGITFDSGGYNLKTGANSNIENMKND 309
           L VAAAAT NPP+FIHL +K P +    KL LVGKG+TFDSGGYNLK GA S IE MKND
Sbjct: 311 LAVAAAATENPPYFIHLCFKTPTKERKTKLALVGKGLTFDSGGYNLKVGAGSRIELMKND 370

Query: 310 MGGAAALLGAAKAIGEIKPLDVEIHFVVAACENMISATGIRPGDIVTASNGKTIEVNNTD 369
           MGGAAA+LGAAKA+GEI+P  VE+HF+VAACENMISA G+RPGDIVTASNGKTIEVNNTD
Sbjct: 371 MGGAAAVLGAAKALGEIRPSRVEVHFIVAACENMISAEGMRPGDIVTASNGKTIEVNNTD 430

Query: 370 AEGRLCLADALIYTCNQGVDKIIDLATLTGACITALGPSVAGAFTPNEDLAKEVFAAAES 429
           AEGRL LADALIY CNQGV+KIIDLATLTGA + ALGPSVAGAFTPN+DLA+EV  AAE+
Sbjct: 431 AEGRLTLADALIYACNQGVEKIIDLATLTGAIMVALGPSVAGAFTPNDDLAREVVEAAEA 490

Query: 430 SGEKIWRLPLEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDEKVQWMHLDIAGP 489
           SGEK+WR+P+EESYWESMKSG  DMINTGPG GGAIT ALFLKQFVDEKVQW+HLD+AGP
Sbjct: 491 SGEKLWRMPMEESYWESMKSGSGDMINTGPGNGGAITGALFLKQFVDEKVQWLHLDVAGP 550

Query: 490 VWNSKKSVATGFGISTLVEWVLKN 514
           VW+ +K  ATG+G+STLVEWVL+N
Sbjct: 551 VWSDEKKNATGYGVSTLVEWVLRN 571

BLAST of MS007215 vs. ExPASy Swiss-Prot
Match: Q6K669 (Leucine aminopeptidase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0794700 PE=2 SV=1)

HSP 1 Score: 666.4 bits (1718), Expect = 2.6e-190
Identity = 334/507 (65.88%), Postives = 411/507 (81.07%), Query Frame = 0

Query: 10  KIKFDAKRLDELEWNGDLLAVGVTQEDVAKDENSIFENPLLYRLDARLNGLLAEACSEED 69
           ++ F AK ++  EW GD+LA+ VT+ D+ K  +S FEN +L +LD +L GLL+EA +EED
Sbjct: 93  QVSFAAKDVEFSEWKGDILAIAVTENDLVKGSDSKFENAVLKKLDGQLGGLLSEASAEED 152

Query: 70  FSGKSGQSLVLRVSGLASKRVGLFGLGQSA-STVAAIQGLGEAIAAAAKASRASSVAVSL 129
           F+GK+GQS+VLR+ G   KRVGL GLGQ+A ST  A +G+GE++A+ AK+++ASS A+  
Sbjct: 153 FTGKAGQSVVLRLPGQGFKRVGLIGLGQNAPSTTTACKGIGESVASVAKSAQASSAAIVF 212

Query: 130 AFSESLVPESKPNVASAIAIGIVLGVFDDNRYRSEPKRTVLQSADILGLGFGPVLDKKLK 189
           A    +  + K   A+AIA G VLG+ +D+RY+SE K+  L+  D++G G GP +D+KLK
Sbjct: 213 ASVGGIQEDFKLTAAAAIASGTVLGLHEDSRYKSESKKVHLKQVDLIGFGSGPEVDQKLK 272

Query: 190 YAQSVSSAIVFGRELVNSPANILTPGELALEALKITSVHSDVLSANIYNEGEIIELKMGS 249
           YA  +SS ++FG+ELVNSPAN+LTP  LA EA  I S +SDV +A I +  +  ELKMGS
Sbjct: 273 YANDLSSGVIFGKELVNSPANVLTPAVLAEEASNIASTYSDVFTATILDVEKCKELKMGS 332

Query: 250 YLGVAAAATANPPHFIHLIYKPPGRPAAIKLGLVGKGITFDSGGYNLKTGANSNIENMKN 309
           YLGV AAA+ANPPHFIHL YKPPG  A  KL +VGKG+TFDSGGYN+KTG   +IE MK 
Sbjct: 333 YLGV-AAASANPPHFIHLCYKPPGGNAKRKLAIVGKGLTFDSGGYNIKTGPGCSIELMKF 392

Query: 310 DMGGAAALLGAAKAIGEIKPLDVEIHFVVAACENMISATGIRPGDIVTASNGKTIEVNNT 369
           DMGG+AA+ GAAKA+G+IKP  VE+HF+VAACENMIS TG+RPGDIVTASNGKTIEVNNT
Sbjct: 393 DMGGSAAVFGAAKALGQIKPPGVEVHFIVAACENMISGTGMRPGDIVTASNGKTIEVNNT 452

Query: 370 DAEGRLCLADALIYTCNQGVDKIIDLATLTGACITALGPSVAGAFTPNEDLAKEVFAAAE 429
           DAEGRL LADAL+Y CNQGVDKIIDLATLTGAC+ ALGPS+AG FTP+++LAKEV AA+E
Sbjct: 453 DAEGRLTLADALVYACNQGVDKIIDLATLTGACVVALGPSIAGIFTPSDELAKEVAAASE 512

Query: 430 SSGEKIWRLPLEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDEKVQWMHLDIAG 489
            SGEK WR+PLEESYWESMKSGVADM+NTG  QGG+ITAALFLKQFVDEKVQWMH+D+AG
Sbjct: 513 ISGEKFWRMPLEESYWESMKSGVADMVNTGGRQGGSITAALFLKQFVDEKVQWMHIDMAG 572

Query: 490 PVWNSKKSVATGFGISTLVEWVLKNAA 516
           PVWN KK  ATGFG+STLVEWVLKN++
Sbjct: 573 PVWNDKKRAATGFGVSTLVEWVLKNSS 598

BLAST of MS007215 vs. ExPASy TrEMBL
Match: A0A6J1E067 (leucine aminopeptidase 2, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111024709 PE=3 SV=1)

HSP 1 Score: 975.7 bits (2521), Expect = 7.5e-281
Identity = 504/513 (98.25%), Postives = 507/513 (98.83%), Query Frame = 0

Query: 3   PVCKNVSKIKFDAKRLDELEWNGDLLAVGVTQEDVAKDENSIFENPLLYRLDARLNGLLA 62
           P   N  KIKFDAK LDELEWNGDLLAVGVTQEDVAKDENSIFENPLLYRLDARLNGLLA
Sbjct: 16  PGPSNAPKIKFDAKHLDELEWNGDLLAVGVTQEDVAKDENSIFENPLLYRLDARLNGLLA 75

Query: 63  EACSEEDFSGKSGQSLVLRVSGLASKRVGLFGLGQSASTVAAIQGLGEAIAAAAKASRAS 122
           EAC+EEDFSGKSGQSLVLRVSGLASKRVGLFGLGQSASTVAAIQGLGEAIAAAAKASRAS
Sbjct: 76  EACAEEDFSGKSGQSLVLRVSGLASKRVGLFGLGQSASTVAAIQGLGEAIAAAAKASRAS 135

Query: 123 SVAVSLAFSESLVPESKPNVASAIAIGIVLGVFDDNRYRSEPKRTVLQSADILGLGFGPV 182
           SVAVSLAFSESLVPESKPNVASAIAIGIVLGVFDDNRYRSEPKRTVLQSADILGLGFGPV
Sbjct: 136 SVAVSLAFSESLVPESKPNVASAIAIGIVLGVFDDNRYRSEPKRTVLQSADILGLGFGPV 195

Query: 183 LDKKLKYAQSVSSAIVFGRELVNSPANILTPGELALEALKITSVHSDVLSANIYNEGEII 242
           LDKKLKYAQSVSSAIVFGRELVNSPANILTPGELALEALKITSVHSDVLSANIYNEGEII
Sbjct: 196 LDKKLKYAQSVSSAIVFGRELVNSPANILTPGELALEALKITSVHSDVLSANIYNEGEII 255

Query: 243 ELKMGSYLGVAAAATANPPHFIHLIYKPPGRPAAIKLGLVGKGITFDSGGYNLKTGANSN 302
           ELKMGSYLG+AAAATANPPHFIHLIYKPPGRPAAIKLGLVGKGITFDSGGYNLKTGANSN
Sbjct: 256 ELKMGSYLGIAAAATANPPHFIHLIYKPPGRPAAIKLGLVGKGITFDSGGYNLKTGANSN 315

Query: 303 IENMKNDMGGAAALLGAAKAIGEIKPLDVEIHFVVAACENMISATGIRPGDIVTASNGKT 362
           IENMKNDMGGAAALLGAAKAIGEIKPLDVEIHFVVAACENMISATG+RPGDIVTASNGKT
Sbjct: 316 IENMKNDMGGAAALLGAAKAIGEIKPLDVEIHFVVAACENMISATGMRPGDIVTASNGKT 375

Query: 363 IEVNNTDAEGRLCLADALIYTCNQGVDKIIDLATLTGACITALGPSVAGAFTPNEDLAKE 422
           IEVNNTDAEGRLCLADALIYTCNQGVDKIIDLATLTGACITALGPSVAGAFTPNEDLAKE
Sbjct: 376 IEVNNTDAEGRLCLADALIYTCNQGVDKIIDLATLTGACITALGPSVAGAFTPNEDLAKE 435

Query: 423 VFAAAESSGEKIWRLPLEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDEKVQWM 482
           VFAAAESSGEKIWRLPLEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDEKVQWM
Sbjct: 436 VFAAAESSGEKIWRLPLEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDEKVQWM 495

Query: 483 HLDIAGPVWNSKKSVATGFGISTLVEWVLKNAA 516
           HLDIAGPVWNSKKSVATGFGISTLVEWVLKNAA
Sbjct: 496 HLDIAGPVWNSKKSVATGFGISTLVEWVLKNAA 528

BLAST of MS007215 vs. ExPASy TrEMBL
Match: A0A6J1KKF4 (leucine aminopeptidase 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111493959 PE=3 SV=1)

HSP 1 Score: 814.7 bits (2103), Expect = 2.2e-232
Identity = 417/513 (81.29%), Postives = 453/513 (88.30%), Query Frame = 0

Query: 3   PVCKNVSKIKFDAKRLDELEWNGDLLAVGVTQEDVAKDENSIFENPLLYRLDARLNGLLA 62
           P   N  KI F AK LD LEWNGDL+AVGV ++DV KDE   F+NPLLY+L+  L GLLA
Sbjct: 14  PGPSNAPKISFVAKDLDVLEWNGDLVAVGVREKDVEKDEAGHFKNPLLYQLNEALGGLLA 73

Query: 63  EACSEEDFSGKSGQSLVLRVSGLASKRVGLFGLGQSASTVAAIQGLGEAIAAAAKASRAS 122
           EA SEEDFSGKS QSLVLRVSGL  KRVGLFGLGQSAS+ AA  GLGEAIAAAA+ASRA+
Sbjct: 74  EASSEEDFSGKSAQSLVLRVSGLRFKRVGLFGLGQSASSAAAFVGLGEAIAAAAQASRAA 133

Query: 123 SVAVSLAFSESLVPESKPNVASAIAIGIVLGVFDDNRYRSEPKRTVLQSADILGLGFGPV 182
           S+A+SLAFSE L  ESKP +ASAIA+GIV G+FDDNRY+S PK T+LQS D+LGLGFGP 
Sbjct: 134 SLALSLAFSEDLSAESKPGIASAIALGIVNGIFDDNRYKSNPKMTLLQSVDVLGLGFGPN 193

Query: 183 LDKKLKYAQSVSSAIVFGRELVNSPANILTPGELALEALKITSVHSDVLSANIYNEGEII 242
           ++KKLKYA+ VSS +VFG+ELVNSPANILTPG+LA E LKIT  + DVLSA I+NE EII
Sbjct: 194 MEKKLKYAEYVSSGVVFGKELVNSPANILTPGQLAGEVLKITHKYHDVLSAKIFNEEEII 253

Query: 243 ELKMGSYLGVAAAATANPPHFIHLIYKPPGRPAAIKLGLVGKGITFDSGGYNLKTGANSN 302
           E+KMGSYLGV AAATANPPHFIHL YKPPG   + KLGLVGKGITFDSGGYNLKTGANSN
Sbjct: 254 EMKMGSYLGVTAAATANPPHFIHLCYKPPGGSVSTKLGLVGKGITFDSGGYNLKTGANSN 313

Query: 303 IENMKNDMGGAAALLGAAKAIGEIKPLDVEIHFVVAACENMISATGIRPGDIVTASNGKT 362
           IE MKNDMGGAAA+ GAAKAI E+KP  VEIHFVVAACENMISATG+RP DIVTASNGKT
Sbjct: 314 IETMKNDMGGAAAIFGAAKAIAELKPPGVEIHFVVAACENMISATGMRPSDIVTASNGKT 373

Query: 363 IEVNNTDAEGRLCLADALIYTCNQGVDKIIDLATLTGACITALGPSVAGAFTPNEDLAKE 422
           IEVNNTDAEGRLCLADALIYTCN GV+KIIDLATLTGACITALGPSVAGAFTPNE+LAKE
Sbjct: 374 IEVNNTDAEGRLCLADALIYTCNLGVEKIIDLATLTGACITALGPSVAGAFTPNEELAKE 433

Query: 423 VFAAAESSGEKIWRLPLEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDEKVQWM 482
           V  AAE SGEKIWRLP+EESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDE VQWM
Sbjct: 434 VIDAAERSGEKIWRLPMEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDENVQWM 493

Query: 483 HLDIAGPVWNSKKSVATGFGISTLVEWVLKNAA 516
           HLDIAGPVWN+KKSVATGFG+STLVEWVLKN +
Sbjct: 494 HLDIAGPVWNTKKSVATGFGVSTLVEWVLKNVS 526

BLAST of MS007215 vs. ExPASy TrEMBL
Match: A0A6J1G4K2 (leucine aminopeptidase 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111450682 PE=3 SV=1)

HSP 1 Score: 811.6 bits (2095), Expect = 1.9e-231
Identity = 416/512 (81.25%), Postives = 451/512 (88.09%), Query Frame = 0

Query: 3   PVCKNVSKIKFDAKRLDELEWNGDLLAVGVTQEDVAKDENSIFENPLLYRLDARLNGLLA 62
           P   N  KI F AK LD LEWNGDL+AVGV ++DV KDE   F+NPLLY+LD  L GLL 
Sbjct: 14  PGPSNAPKITFVAKDLDVLEWNGDLVAVGVREKDVEKDEAGHFKNPLLYQLDEALGGLLG 73

Query: 63  EACSEEDFSGKSGQSLVLRVSGLASKRVGLFGLGQSASTVAAIQGLGEAIAAAAKASRAS 122
           EA SEEDFSGKS QSLVLRVSGL  KRVGLFGLGQSAS+ AA  GLGEAIAAAA+ SRA+
Sbjct: 74  EASSEEDFSGKSAQSLVLRVSGLKFKRVGLFGLGQSASSAAAFVGLGEAIAAAAQTSRAA 133

Query: 123 SVAVSLAFSESLVPESKPNVASAIAIGIVLGVFDDNRYRSEPKRTVLQSADILGLGFGPV 182
           S+A++LAFSE L  ESKP +ASAIA+GIV G+FDDNRY+S PK TVLQ  D+LGLGFGP 
Sbjct: 134 SLALALAFSEDLSAESKPGIASAIALGIVNGIFDDNRYKSNPKMTVLQFVDVLGLGFGPN 193

Query: 183 LDKKLKYAQSVSSAIVFGRELVNSPANILTPGELALEALKITSVHSDVLSANIYNEGEII 242
           ++KKLKYA+ VSS +VFG+ELVNSPANILTPG+LA E LKIT  ++DVLSA I+NE EII
Sbjct: 194 MEKKLKYAEYVSSGVVFGKELVNSPANILTPGQLAGEVLKITHKYNDVLSAKIFNEEEII 253

Query: 243 ELKMGSYLGVAAAATANPPHFIHLIYKPPGRPAAIKLGLVGKGITFDSGGYNLKTGANSN 302
           E+KMGSYLGV AAATANPPHFIHL YKP G   + KLGLVGKGITFDSGGYNLKTGANSN
Sbjct: 254 EMKMGSYLGVTAAATANPPHFIHLCYKPLGGSVSTKLGLVGKGITFDSGGYNLKTGANSN 313

Query: 303 IENMKNDMGGAAALLGAAKAIGEIKPLDVEIHFVVAACENMISATGIRPGDIVTASNGKT 362
           IE MKNDMGGAAA+ GAAKAI E+KP  VEIHFVVAACENMISATG+RP DIVTASNGKT
Sbjct: 314 IETMKNDMGGAAAIFGAAKAIAELKPPGVEIHFVVAACENMISATGMRPSDIVTASNGKT 373

Query: 363 IEVNNTDAEGRLCLADALIYTCNQGVDKIIDLATLTGACITALGPSVAGAFTPNEDLAKE 422
           IEVNNTDAEGRLCLADALIYTCN GV+KIIDLATLTGACITALGPSVAGAFTPNE+LAKE
Sbjct: 374 IEVNNTDAEGRLCLADALIYTCNLGVEKIIDLATLTGACITALGPSVAGAFTPNEELAKE 433

Query: 423 VFAAAESSGEKIWRLPLEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDEKVQWM 482
           V AAAE SGEKIWRLP+EESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDE VQWM
Sbjct: 434 VLAAAERSGEKIWRLPMEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDENVQWM 493

Query: 483 HLDIAGPVWNSKKSVATGFGISTLVEWVLKNA 515
           HLDIAGPVWN+KKSVATGFG+STLVEWVLKNA
Sbjct: 494 HLDIAGPVWNTKKSVATGFGVSTLVEWVLKNA 525

BLAST of MS007215 vs. ExPASy TrEMBL
Match: A0A0A0LK31 (CYTOSOL_AP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G035470 PE=3 SV=1)

HSP 1 Score: 790.8 bits (2041), Expect = 3.4e-225
Identity = 408/513 (79.53%), Postives = 444/513 (86.55%), Query Frame = 0

Query: 3   PVCKNVSKIKFDAKRLDELEWNGDLLAVGVTQEDVAKDENSIFENPLLYRLDARLNGLLA 62
           P   N  KI F AK  D LEWNGDLLAVGVT++DV KD+N+ F N LL +LDA L GLLA
Sbjct: 14  PGPSNAPKITFAAKDTDVLEWNGDLLAVGVTEKDVVKDDNNNFTNSLLKKLDAVLGGLLA 73

Query: 63  EACSEEDFSGKSGQSLVLRVSGLASKRVGLFGLGQSASTVAAIQGLGEAIAAAAKASRAS 122
           EA SEEDFSGKSGQ++VLRVSGL+ KRVGLFGLGQSA + AA  GLGEAIAAAA+ASRA 
Sbjct: 74  EASSEEDFSGKSGQAIVLRVSGLSFKRVGLFGLGQSAYSAAAFVGLGEAIAAAAQASRAV 133

Query: 123 SVAVSLAFSESLVPESKPNVASAIAIGIVLGVFDDNRYRSEPKRTVLQSADILGLGFGPV 182
           S+AV+LAFS  L  ESKP++AS+IAIGIV G+FDDNRYRS PK TVLQS D+LGLG G  
Sbjct: 134 SLAVALAFSADLSDESKPDIASSIAIGIVNGIFDDNRYRSNPKATVLQSVDVLGLGSGAA 193

Query: 183 LDKKLKYAQSVSSAIVFGRELVNSPANILTPGELALEALKITSVHSDVLSANIYNEGEII 242
           + KKLKYAQ VSS IVF RELVNSPANILTPG+LA E L I   +SDVLSA I+NE EII
Sbjct: 194 MIKKLKYAQYVSSGIVFVRELVNSPANILTPGQLAAEVLNIARNYSDVLSARIFNEDEII 253

Query: 243 ELKMGSYLGVAAAATANPPHFIHLIYKPPGRPAAIKLGLVGKGITFDSGGYNLKTGANSN 302
           E+KMGSYLGV AAATANPP FIHL YKPP    + KLGLVGKGITFDSGGYNLKTGANSN
Sbjct: 254 EMKMGSYLGVTAAATANPPKFIHLCYKPPSGCVSAKLGLVGKGITFDSGGYNLKTGANSN 313

Query: 303 IENMKNDMGGAAALLGAAKAIGEIKPLDVEIHFVVAACENMISATGIRPGDIVTASNGKT 362
           IE MKNDMGGA A+ GAAKAI EIKPL VEIHFVVAACENMISATG+RP DIVTA+NGKT
Sbjct: 314 IETMKNDMGGAGAIFGAAKAIAEIKPLGVEIHFVVAACENMISATGMRPSDIVTAANGKT 373

Query: 363 IEVNNTDAEGRLCLADALIYTCNQGVDKIIDLATLTGACITALGPSVAGAFTPNEDLAKE 422
           IEVNNTDAEGRLCLADALIYTC  GVDKIIDLATLTGACI ALGPSVAGAFTPNE+LA E
Sbjct: 374 IEVNNTDAEGRLCLADALIYTCKLGVDKIIDLATLTGACIIALGPSVAGAFTPNEELASE 433

Query: 423 VFAAAESSGEKIWRLPLEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDEKVQWM 482
           V +AAE SGEK+WRLP+EESYW+SMKSGVADM+NTGP QGGAITAALFLKQFVDE VQWM
Sbjct: 434 VLSAAERSGEKLWRLPIEESYWDSMKSGVADMLNTGPSQGGAITAALFLKQFVDENVQWM 493

Query: 483 HLDIAGPVWNSKKSVATGFGISTLVEWVLKNAA 516
           HLDIAGPVWN+KKS+ATGFG+STLVEWVLKNA+
Sbjct: 494 HLDIAGPVWNTKKSIATGFGVSTLVEWVLKNAS 526

BLAST of MS007215 vs. ExPASy TrEMBL
Match: A0A6J1KBF4 (leucine aminopeptidase 2, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493948 PE=3 SV=1)

HSP 1 Score: 785.8 bits (2028), Expect = 1.1e-223
Identity = 403/513 (78.56%), Postives = 441/513 (85.96%), Query Frame = 0

Query: 3   PVCKNVSKIKFDAKRLDELEWNGDLLAVGVTQEDVAKDENSIFENPLLYRLDARLNGLLA 62
           P   N  KI F AK LD LEWNGDL+AVGV ++DV KDE   F+NPLLY+L+  L GLLA
Sbjct: 14  PGPSNAPKIIFVAKDLDVLEWNGDLVAVGVREKDVEKDEAGHFKNPLLYQLNEALGGLLA 73

Query: 63  EACSEEDFSGKSGQSLVLRVSGLASKRVGLFGLGQSASTVAAIQGLGEAIAAAAKASRAS 122
           EA SEEDFSGKS QSLVLRVSGL  KRVGLFGLGQSAS+ AA  GLGEAIAAAA+ASRA+
Sbjct: 74  EASSEEDFSGKSAQSLVLRVSGLRFKRVGLFGLGQSASSAAAFVGLGEAIAAAAQASRAA 133

Query: 123 SVAVSLAFSESLVPESKPNVASAIAIGIVLGVFDDNRYRSEPKRTVLQSADILGLGFGPV 182
           S+A+SLAFSE L  ESKP +ASAIA+GIV G+FDDNRY+S PK T+LQS D+LGLGFGP 
Sbjct: 134 SLALSLAFSEDLSAESKPGIASAIALGIVNGIFDDNRYKSNPKMTLLQSVDVLGLGFGPN 193

Query: 183 LDKKLKYAQSVSSAIVFGRELVNSPANILTPGELALEALKITSVHSDVLSANIYNEGEII 242
           ++KKLKYA+ VSS +VFG+ELVNSPANILTPGE A E LKI   ++DVLSA I+ E  +I
Sbjct: 194 MEKKLKYAEYVSSGVVFGKELVNSPANILTPGEFAGEVLKIAEEYNDVLSAKIFKEDRMI 253

Query: 243 ELKMGSYLGVAAAATANPPHFIHLIYKPPGRPAAIKLGLVGKGITFDSGGYNLKTGANSN 302
           ELKMGSYLGV AAATANP HFIHL YKPP  P   KLGLVGKGITFDSGGYNLKTG NSN
Sbjct: 254 ELKMGSYLGVTAAATANPAHFIHLCYKPPCGPVLTKLGLVGKGITFDSGGYNLKTGPNSN 313

Query: 303 IENMKNDMGGAAALLGAAKAIGEIKPLDVEIHFVVAACENMISATGIRPGDIVTASNGKT 362
           IE MK DMGGAAA+ GAAKAI E+KP  VEIHFVV ACENMISATG+RP DIVTASNGKT
Sbjct: 314 IETMKKDMGGAAAIFGAAKAIAELKPPGVEIHFVVPACENMISATGMRPSDIVTASNGKT 373

Query: 363 IEVNNTDAEGRLCLADALIYTCNQGVDKIIDLATLTGACITALGPSVAGAFTPNEDLAKE 422
           IEVNNTDAEGRLCLADAL YTCN GV+KIIDLATLTGACI ALGPSVAGAFTPNE+LAKE
Sbjct: 374 IEVNNTDAEGRLCLADALFYTCNLGVEKIIDLATLTGACIMALGPSVAGAFTPNEELAKE 433

Query: 423 VFAAAESSGEKIWRLPLEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDEKVQWM 482
           V  AAE SGEKIWRLP+EESYWE MKSGVADMINTGPGQGGAIT ALFLKQFV + VQWM
Sbjct: 434 VIDAAERSGEKIWRLPMEESYWEFMKSGVADMINTGPGQGGAITGALFLKQFVADNVQWM 493

Query: 483 HLDIAGPVWNSKKSVATGFGISTLVEWVLKNAA 516
           HLDIAGPVWN++KS+ATGFG+STLVEWVLKNA+
Sbjct: 494 HLDIAGPVWNARKSIATGFGVSTLVEWVLKNAS 526

BLAST of MS007215 vs. TAIR 10
Match: AT4G30920.1 (Cytosol aminopeptidase family protein )

HSP 1 Score: 699.9 bits (1805), Expect = 1.5e-201
Identity = 354/506 (69.96%), Postives = 419/506 (82.81%), Query Frame = 0

Query: 10  KIKFDAKRLDELEWNGDLLAVGVTQEDVAKDENSIFENPLLYRLDARLNGLLAEACSEED 69
           KI F  K +D  EW GD+LAVGVT++D+AKD NS FENP+L +LDA L GLLA+  SEED
Sbjct: 78  KISFSGKEIDVTEWKGDILAVGVTEKDMAKDVNSKFENPILKKLDAHLGGLLADVSSEED 137

Query: 70  FSGKSGQSLVLRVSGLASKRVGLFGLGQSASTVAAIQGLGEAIAAAAKASRASSVAVSLA 129
           FSGK GQS VLR+ GL SKRVGL GLG+SAST +A Q LGEA+AAAAKAS+ASSVAV LA
Sbjct: 138 FSGKPGQSTVLRLPGLGSKRVGLIGLGKSASTPSAFQSLGEAVAAAAKASQASSVAVVLA 197

Query: 130 FSESLVPESKPNVASAIAIGIVLGVFDDNRYRSEPKRTVLQSADILGLGFGPVLDKKLKY 189
            SES+  ESK   ASAIA G VLG+F+D+RY+SE K+  L+S DI+G G GP L+KKLKY
Sbjct: 198 SSESVSNESKLCSASAIASGTVLGLFEDSRYKSESKKPSLKSVDIIGFGSGPELEKKLKY 257

Query: 190 AQSVSSAIVFGRELVNSPANILTPGELALEALKITSVHSDVLSANIYNEGEIIELKMGSY 249
           A+ VS  ++FG+ELVNSPAN+LTP  LA EAL + S++SDV++ANI NE +  ELKMGSY
Sbjct: 258 AEHVSYGVIFGKELVNSPANVLTPAVLAEEALNLASMYSDVMTANILNEEQCKELKMGSY 317

Query: 250 LGVAAAATANPPHFIHLIYKPPGRPAAIKLGLVGKGITFDSGGYNLKTGANSNIENMKND 309
           L V AAA+ANPPHFIHLIYKP   P   KL LVGKG+TFDSGGYN+KTG    IE MK D
Sbjct: 318 LAV-AAASANPPHFIHLIYKPSSGPVKTKLALVGKGLTFDSGGYNIKTGPGCLIELMKFD 377

Query: 310 MGGAAALLGAAKAIGEIKPLDVEIHFVVAACENMISATGIRPGDIVTASNGKTIEVNNTD 369
           MGG+AA+LGAAKAIG+IKP  VE+HF+VAACENMIS TG+RPGD++TASNGKTIEVNNTD
Sbjct: 378 MGGSAAVLGAAKAIGQIKPPGVEVHFIVAACENMISGTGMRPGDVLTASNGKTIEVNNTD 437

Query: 370 AEGRLCLADALIYTCNQGVDKIIDLATLTGACITALGPSVAGAFTPNEDLAKEVFAAAES 429
           AEGRL LADAL+Y CNQGVDK++DLATLTGACI ALG S+AG +TP++ LAKEV AA+E 
Sbjct: 438 AEGRLTLADALVYACNQGVDKVVDLATLTGACIIALGTSMAGIYTPSDKLAKEVIAASER 497

Query: 430 SGEKIWRLPLEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDEKVQWMHLDIAGP 489
           SGEK+WR+P+EESYWE MKSGVADM+NTG   GG+ITAALFLKQFV E V+WMH+D+AGP
Sbjct: 498 SGEKLWRMPMEESYWEMMKSGVADMVNTGGRAGGSITAALFLKQFVSEDVEWMHIDMAGP 557

Query: 490 VWNSKKSVATGFGISTLVEWVLKNAA 516
           VWN KK  ATGFG++TLVEWV  +++
Sbjct: 558 VWNEKKKAATGFGVATLVEWVQNHSS 582

BLAST of MS007215 vs. TAIR 10
Match: AT2G24200.1 (Cytosol aminopeptidase family protein )

HSP 1 Score: 682.6 bits (1760), Expect = 2.5e-196
Identity = 340/506 (67.19%), Postives = 412/506 (81.42%), Query Frame = 0

Query: 10  KIKFDAKRLDELEWNGDLLAVGVTQEDVAKDENSIFENPLLYRLDARLNGLLAEACSEED 69
           KI F AK +D +EW GD+L VGVT++D+AKD NS FENP+L ++DA L+GLLA+  SEED
Sbjct: 17  KISFTAKEIDVIEWKGDILVVGVTEKDLAKDGNSKFENPILSKVDAHLSGLLAQVSSEED 76

Query: 70  FSGKSGQSLVLRVSGLASKRVGLFGLGQSASTVAAIQGLGEAIAAAAKASRASSVAVSLA 129
           F+GK GQS VLR+ GL SKR+ L GLGQS S+  A   LGEA+A  +KAS+++S A+ LA
Sbjct: 77  FTGKPGQSTVLRLPGLGSKRIALIGLGQSVSSPVAFHSLGEAVATVSKASQSTSAAIVLA 136

Query: 130 FSESLVPESKPNVASAIAIGIVLGVFDDNRYRSEPKRTVLQSADILGLGFGPVLDKKLKY 189
            S S   ESK +  SA+A GIVLG+F+D RY+SE K+  L++ DI+G G G  ++KKLKY
Sbjct: 137 SSVS--DESKLSSVSALASGIVLGLFEDGRYKSESKKPSLKAVDIIGFGTGAEVEKKLKY 196

Query: 190 AQSVSSAIVFGRELVNSPANILTPGELALEALKITSVHSDVLSANIYNEGEIIELKMGSY 249
           A+ VS  ++FGREL+NSPAN+LTP  LA EA K+ S +SDV +ANI NE +  ELKMGSY
Sbjct: 197 AEDVSYGVIFGRELINSPANVLTPAVLAEEAAKVASTYSDVFTANILNEEQCKELKMGSY 256

Query: 250 LGVAAAATANPPHFIHLIYKPPGRPAAIKLGLVGKGITFDSGGYNLKTGANSNIENMKND 309
           L V AAA+ANPPHFIHL+YKPP      KL LVGKG+TFDSGGYN+KTG   +IE MK D
Sbjct: 257 LAV-AAASANPPHFIHLVYKPPNGSVKTKLALVGKGLTFDSGGYNIKTGPGCSIELMKFD 316

Query: 310 MGGAAALLGAAKAIGEIKPLDVEIHFVVAACENMISATGIRPGDIVTASNGKTIEVNNTD 369
           MGG+AA+LGAAKAIGEIKP  VE+HF+VAACENMIS TG+RPGD++TASNGKTIEVNNTD
Sbjct: 317 MGGSAAVLGAAKAIGEIKPPGVEVHFIVAACENMISGTGMRPGDVITASNGKTIEVNNTD 376

Query: 370 AEGRLCLADALIYTCNQGVDKIIDLATLTGACITALGPSVAGAFTPNEDLAKEVFAAAES 429
           AEGRL LADAL+Y CNQGVDKI+DLATLTGAC+ ALG S+AG +TP+++LAKEV AA+E 
Sbjct: 377 AEGRLTLADALVYACNQGVDKIVDLATLTGACVIALGTSMAGIYTPSDELAKEVIAASER 436

Query: 430 SGEKIWRLPLEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDEKVQWMHLDIAGP 489
           SGEK+WR+PLEESYWE MKSGVADM+NTG   GG+ITAALFLKQFV EKVQWMH+D+AGP
Sbjct: 437 SGEKLWRMPLEESYWEMMKSGVADMVNTGGRAGGSITAALFLKQFVSEKVQWMHIDMAGP 496

Query: 490 VWNSKKSVATGFGISTLVEWVLKNAA 516
           VWN KK   TGFG++TLVEWV KN++
Sbjct: 497 VWNEKKKSGTGFGVATLVEWVQKNSS 519

BLAST of MS007215 vs. TAIR 10
Match: AT2G24200.2 (Cytosol aminopeptidase family protein )

HSP 1 Score: 682.6 bits (1760), Expect = 2.5e-196
Identity = 340/506 (67.19%), Postives = 412/506 (81.42%), Query Frame = 0

Query: 10  KIKFDAKRLDELEWNGDLLAVGVTQEDVAKDENSIFENPLLYRLDARLNGLLAEACSEED 69
           KI F AK +D +EW GD+L VGVT++D+AKD NS FENP+L ++DA L+GLLA+  SEED
Sbjct: 17  KISFTAKEIDVIEWKGDILVVGVTEKDLAKDGNSKFENPILSKVDAHLSGLLAQVSSEED 76

Query: 70  FSGKSGQSLVLRVSGLASKRVGLFGLGQSASTVAAIQGLGEAIAAAAKASRASSVAVSLA 129
           F+GK GQS VLR+ GL SKR+ L GLGQS S+  A   LGEA+A  +KAS+++S A+ LA
Sbjct: 77  FTGKPGQSTVLRLPGLGSKRIALIGLGQSVSSPVAFHSLGEAVATVSKASQSTSAAIVLA 136

Query: 130 FSESLVPESKPNVASAIAIGIVLGVFDDNRYRSEPKRTVLQSADILGLGFGPVLDKKLKY 189
            S S   ESK +  SA+A GIVLG+F+D RY+SE K+  L++ DI+G G G  ++KKLKY
Sbjct: 137 SSVS--DESKLSSVSALASGIVLGLFEDGRYKSESKKPSLKAVDIIGFGTGAEVEKKLKY 196

Query: 190 AQSVSSAIVFGRELVNSPANILTPGELALEALKITSVHSDVLSANIYNEGEIIELKMGSY 249
           A+ VS  ++FGREL+NSPAN+LTP  LA EA K+ S +SDV +ANI NE +  ELKMGSY
Sbjct: 197 AEDVSYGVIFGRELINSPANVLTPAVLAEEAAKVASTYSDVFTANILNEEQCKELKMGSY 256

Query: 250 LGVAAAATANPPHFIHLIYKPPGRPAAIKLGLVGKGITFDSGGYNLKTGANSNIENMKND 309
           L V AAA+ANPPHFIHL+YKPP      KL LVGKG+TFDSGGYN+KTG   +IE MK D
Sbjct: 257 LAV-AAASANPPHFIHLVYKPPNGSVKTKLALVGKGLTFDSGGYNIKTGPGCSIELMKFD 316

Query: 310 MGGAAALLGAAKAIGEIKPLDVEIHFVVAACENMISATGIRPGDIVTASNGKTIEVNNTD 369
           MGG+AA+LGAAKAIGEIKP  VE+HF+VAACENMIS TG+RPGD++TASNGKTIEVNNTD
Sbjct: 317 MGGSAAVLGAAKAIGEIKPPGVEVHFIVAACENMISGTGMRPGDVITASNGKTIEVNNTD 376

Query: 370 AEGRLCLADALIYTCNQGVDKIIDLATLTGACITALGPSVAGAFTPNEDLAKEVFAAAES 429
           AEGRL LADAL+Y CNQGVDKI+DLATLTGAC+ ALG S+AG +TP+++LAKEV AA+E 
Sbjct: 377 AEGRLTLADALVYACNQGVDKIVDLATLTGACVIALGTSMAGIYTPSDELAKEVIAASER 436

Query: 430 SGEKIWRLPLEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDEKVQWMHLDIAGP 489
           SGEK+WR+PLEESYWE MKSGVADM+NTG   GG+ITAALFLKQFV EKVQWMH+D+AGP
Sbjct: 437 SGEKLWRMPLEESYWEMMKSGVADMVNTGGRAGGSITAALFLKQFVSEKVQWMHIDMAGP 496

Query: 490 VWNSKKSVATGFGISTLVEWVLKNAA 516
           VWN KK   TGFG++TLVEWV KN++
Sbjct: 497 VWNEKKKSGTGFGVATLVEWVQKNSS 519

BLAST of MS007215 vs. TAIR 10
Match: AT4G30910.1 (Cytosol aminopeptidase family protein )

HSP 1 Score: 662.5 bits (1708), Expect = 2.7e-190
Identity = 335/506 (66.21%), Postives = 408/506 (80.63%), Query Frame = 0

Query: 10  KIKFDAKRLDELEWNGDLLAVGVTQEDVAKDENSIFENPLLYRLDARLNGLLAEACSEED 69
           KI F  K +D  EW GD+LAVGVT++D+AKD NS FENP+L +LDA L GLLA+  +EED
Sbjct: 77  KISFSGKEIDVTEWKGDILAVGVTEKDMAKDANSKFENPILKKLDAHLGGLLADVSAEED 136

Query: 70  FSGKSGQSLVLRVSGLASKRVGLFGLGQSASTVAAIQGLGEAIAAAAKASRASSVAVSLA 129
           FSGK GQS VLR+ GL SKRVGL GLG+SAST +A Q LGEA+AAAAKAS+ASSVAV LA
Sbjct: 137 FSGKPGQSTVLRLPGLGSKRVGLIGLGKSASTPSAFQSLGEAVAAAAKASQASSVAVVLA 196

Query: 130 FSESLVPESKPNVASAIAIGIVLGVFDDNRYRSEPKRTVLQSADILGLGFGPVLDKKLKY 189
            SES   ESK + AS IA G VLG+F+D+RY+SE K+  L+S   +G G GP L+ KLKY
Sbjct: 197 SSESFSDESKLSSASDIASGTVLGLFEDSRYKSESKKPSLKSVVFIGFGTGPELENKLKY 256

Query: 190 AQSVSSAIVFGRELVNSPANILTPGELALEALKITSVHSDVLSANIYNEGEIIELKMGSY 249
           A+ VS  ++F +ELVNSPAN+L+P  LA EA  + S++S+V++ANI  E +  ELKMGSY
Sbjct: 257 AEHVSYGVIFTKELVNSPANVLSPAVLAEEASNLASMYSNVMTANILKEEQCKELKMGSY 316

Query: 250 LGVAAAATANPPHFIHLIYKPPGRPAAIKLGLVGKGITFDSGGYNLKTGANSNIENMKND 309
           L V AAA+ANPPHFIHLIYKP   P   KL LVGKG+TFDSGGYN+K G    IE MK D
Sbjct: 317 LAV-AAASANPPHFIHLIYKPSSGPVKTKLALVGKGLTFDSGGYNIKIGPELIIELMKID 376

Query: 310 MGGAAALLGAAKAIGEIKPLDVEIHFVVAACENMISATGIRPGDIVTASNGKTIEVNNTD 369
           +GG+AA+LGAAKAIGEIKP  VE+HF+VAACENMIS TG+RPGD++TASNGKTIEVN+TD
Sbjct: 377 VGGSAAVLGAAKAIGEIKPPGVEVHFIVAACENMISGTGMRPGDVITASNGKTIEVNDTD 436

Query: 370 AEGRLCLADALIYTCNQGVDKIIDLATLTGACITALGPSVAGAFTPNEDLAKEVFAAAES 429
           +EGRL LADAL+Y CNQGVDKI+D+ATLTG  I ALGPS+AG +T +++LAKEV AA++ 
Sbjct: 437 SEGRLTLADALVYACNQGVDKIVDIATLTGEIIVALGPSMAGMYTASDELAKEVIAASQR 496

Query: 430 SGEKIWRLPLEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDEKVQWMHLDIAGP 489
           SGEK+WR+P+EESYWE MKSGVADM+N G   GG+ITAALFLK+FV E V+W+H+D+AG 
Sbjct: 497 SGEKLWRMPMEESYWEMMKSGVADMVNFGGRAGGSITAALFLKRFVSENVEWLHIDMAGR 556

Query: 490 VWNSKKSVATGFGISTLVEWVLKNAA 516
           VWN KK  ATGFG++TLVEWV  N++
Sbjct: 557 VWNEKKKAATGFGVATLVEWVQNNSS 581

BLAST of MS007215 vs. TAIR 10
Match: AT2G24200.3 (Cytosol aminopeptidase family protein )

HSP 1 Score: 617.5 bits (1591), Expect = 1.0e-176
Identity = 318/506 (62.85%), Postives = 383/506 (75.69%), Query Frame = 0

Query: 10  KIKFDAKRLDELEWNGDLLAVGVTQEDVAKDENSIFENPLLYRLDARLNGLLAEACSEED 69
           KI F AK +D +EW GD+L VGVT++D+AKD NS FENP+L ++DA L+GLLA+  SEED
Sbjct: 17  KISFTAKEIDVIEWKGDILVVGVTEKDLAKDGNSKFENPILSKVDAHLSGLLAQVSSEED 76

Query: 70  FSGKSGQSLVLRVSGLASKRVGLFGLGQSASTVAAIQGLGEAIAAAAKASRASSVAVSLA 129
           F+GK GQS VLR+ GL SKR+ L GLGQS S+  A   LGEA+A  +KAS+++S A+ LA
Sbjct: 77  FTGKPGQSTVLRLPGLGSKRIALIGLGQSVSSPVAFHSLGEAVATVSKASQSTSAAIVLA 136

Query: 130 FSESLVPESKPNVASAIAIGIVLGVFDDNRYRSEPKRTVLQSADILGLGFGPVLDKKLKY 189
            S S   ESK +  SA+A GIVLG+F+D RY+SE K+  L++ DI+G G G         
Sbjct: 137 SSVS--DESKLSSVSALASGIVLGLFEDGRYKSESKKPSLKAVDIIGFGTG--------- 196

Query: 190 AQSVSSAIVFGRELVNSPANILTPGELALEALKITSVHSDVLSANIYNEGEIIELKMGSY 249
                                      A EA K+ S +SDV +ANI NE +  ELKMGSY
Sbjct: 197 ---------------------------AEEAAKVASTYSDVFTANILNEEQCKELKMGSY 256

Query: 250 LGVAAAATANPPHFIHLIYKPPGRPAAIKLGLVGKGITFDSGGYNLKTGANSNIENMKND 309
           L V AAA+ANPPHFIHL+YKPP      KL LVGKG+TFDSGGYN+KTG   +IE MK D
Sbjct: 257 LAV-AAASANPPHFIHLVYKPPNGSVKTKLALVGKGLTFDSGGYNIKTGPGCSIELMKFD 316

Query: 310 MGGAAALLGAAKAIGEIKPLDVEIHFVVAACENMISATGIRPGDIVTASNGKTIEVNNTD 369
           MGG+AA+LGAAKAIGEIKP  VE+HF+VAACENMIS TG+RPGD++TASNGKTIEVNNTD
Sbjct: 317 MGGSAAVLGAAKAIGEIKPPGVEVHFIVAACENMISGTGMRPGDVITASNGKTIEVNNTD 376

Query: 370 AEGRLCLADALIYTCNQGVDKIIDLATLTGACITALGPSVAGAFTPNEDLAKEVFAAAES 429
           AEGRL LADAL+Y CNQGVDKI+DLATLTGAC+ ALG S+AG +TP+++LAKEV AA+E 
Sbjct: 377 AEGRLTLADALVYACNQGVDKIVDLATLTGACVIALGTSMAGIYTPSDELAKEVIAASER 436

Query: 430 SGEKIWRLPLEESYWESMKSGVADMINTGPGQGGAITAALFLKQFVDEKVQWMHLDIAGP 489
           SGEK+WR+PLEESYWE MKSGVADM+NTG   GG+ITAALFLKQFV EKVQWMH+D+AGP
Sbjct: 437 SGEKLWRMPLEESYWEMMKSGVADMVNTGGRAGGSITAALFLKQFVSEKVQWMHIDMAGP 483

Query: 490 VWNSKKSVATGFGISTLVEWVLKNAA 516
           VWN KK   TGFG++TLVEWV KN++
Sbjct: 497 VWNEKKKSGTGFGVATLVEWVQKNSS 483

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158161.11.5e-28098.25leucine aminopeptidase 2, chloroplastic-like [Momordica charantia][more]
XP_022999678.14.6e-23281.29leucine aminopeptidase 2, chloroplastic-like [Cucurbita maxima][more]
XP_022946689.13.9e-23181.25leucine aminopeptidase 2, chloroplastic-like [Cucurbita moschata][more]
KAG6599158.11.5e-23080.90Leucine aminopeptidase 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023545271.17.3e-23080.70leucine aminopeptidase 2, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q944P72.1e-20069.96Leucine aminopeptidase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LAP2 ... [more]
P314271.0e-19768.91Leucine aminopeptidase, chloroplastic OS=Solanum tuberosum OX=4113 GN=LAP PE=2 S... [more]
P301843.6e-19567.19Leucine aminopeptidase 1 OS=Arabidopsis thaliana OX=3702 GN=LAP1 PE=1 SV=1[more]
Q107123.6e-19568.45Leucine aminopeptidase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=LAPA1... [more]
Q6K6692.6e-19065.88Leucine aminopeptidase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947... [more]
Match NameE-valueIdentityDescription
A0A6J1E0677.5e-28198.25leucine aminopeptidase 2, chloroplastic-like OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1KKF42.2e-23281.29leucine aminopeptidase 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1G4K21.9e-23181.25leucine aminopeptidase 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A0A0LK313.4e-22579.53CYTOSOL_AP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G035470 ... [more]
A0A6J1KBF41.1e-22378.56leucine aminopeptidase 2, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3... [more]
Match NameE-valueIdentityDescription
AT4G30920.11.5e-20169.96Cytosol aminopeptidase family protein [more]
AT2G24200.12.5e-19667.19Cytosol aminopeptidase family protein [more]
AT2G24200.22.5e-19667.19Cytosol aminopeptidase family protein [more]
AT4G30910.12.7e-19066.21Cytosol aminopeptidase family protein [more]
AT2G24200.31.0e-17662.85Cytosol aminopeptidase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011356Peptidase M17, leucine aminopeptidase/peptidase BPRINTSPR00481LAMNOPPTDASEcoord: 363..383
score: 72.31
coord: 391..406
score: 70.83
coord: 279..296
score: 72.43
coord: 341..362
score: 57.74
coord: 303..324
score: 54.04
IPR011356Peptidase M17, leucine aminopeptidase/peptidase BPANTHERPTHR11963LEUCINE AMINOPEPTIDASE-RELATEDcoord: 6..513
IPR011356Peptidase M17, leucine aminopeptidase/peptidase BCDDcd00433Peptidase_M17coord: 26..510
e-value: 7.66766E-163
score: 468.179
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 185..513
e-value: 3.3E-131
score: 439.3
NoneNo IPR availablePANTHERPTHR11963:SF43LEUCINE AMINOPEPTIDASE 1, CHLOROPLASTICcoord: 6..513
NoneNo IPR availableSUPERFAMILY53187Zn-dependent exopeptidasescoord: 195..512
IPR043472Macro domain-likeGENE3D3.40.220.10Leucine Aminopeptidase, subunit E, domain 1coord: 2..184
e-value: 2.4E-49
score: 169.6
IPR043472Macro domain-likeSUPERFAMILY52949Macro domain-likecoord: 11..196
IPR000819Peptidase M17, leucyl aminopeptidase, C-terminalPFAMPF00883Peptidase_M17coord: 200..507
e-value: 2.4E-109
score: 365.4
IPR000819Peptidase M17, leucyl aminopeptidase, C-terminalPROSITEPS00631CYTOSOL_APcoord: 367..374
IPR008283Peptidase M17, leucyl aminopeptidase, N-terminalPFAMPF02789Peptidase_M17_Ncoord: 30..163
e-value: 1.5E-22
score: 79.8
IPR023042Peptidase M17, leucine aminopeptidaseHAMAPMF_00181Cytosol_peptidase_M17coord: 13..515
score: 31.440704

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS007215.1MS007215.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019538 protein metabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0005737 cytoplasm
molecular_function GO:0030145 manganese ion binding
molecular_function GO:0070006 metalloaminopeptidase activity