MS007107 (gene) Bitter gourd (TR) v1

BLAST of MS007107 vs. NCBI nr
Match: XP_022149862.1 (DNA ligase 4 isoform X1 [Momordica charantia])

HSP 1 Score: 2405.6 bits (6233), Expect = 0.0e+00
Identity = 1220/1257 (97.06%), Postives = 1232/1257 (98.01%), Query Frame = 0

Query: 1    MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT 60
            MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT
Sbjct: 1    MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT 60

Query: 61   YGLKESVLATCLIDALGISRDSDDALRLTNWRKGGAKTGANAGNFALVAAEVLQRRQGMT 120
            YGLKESVLATCLIDALGISRDSDDALRL NWRKGGAKTGANAGNFALVAAEVLQRRQGMT
Sbjct: 61   YGLKESVLATCLIDALGISRDSDDALRLNNWRKGGAKTGANAGNFALVAAEVLQRRQGMT 120

Query: 121  PGGLTIRELNDLLDRLALSENSRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK 180
            PGGLTI+ELNDLLDRLALSENSRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK
Sbjct: 121  PGGLTIKELNDLLDRLALSENSRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK 180

Query: 181  SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW 240
            SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW
Sbjct: 181  SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW 240

Query: 241  KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENILATRCIL 300
            KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENI+ATRCIL
Sbjct: 241  KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENIIATRCIL 300

Query: 301  DGEMLVWDKSLNRFAEFGSNQEIG-----------EWTDVAFDVLYVGDTSVIHRSLKER 360
            DGEMLVWDKSLNRFAEFGSNQEI            +   VAFDVLYVGDTSVIHRSLKER
Sbjct: 301  DGEMLVWDKSLNRFAEFGSNQEIARAAKDGLDSDRQLCYVAFDVLYVGDTSVIHRSLKER 360

Query: 361  HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV 420
            HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV
Sbjct: 361  HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV 420

Query: 421  IKDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS 480
            IKD+GSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS
Sbjct: 421  IKDMGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS 480

Query: 481  PSNAYPRRFISFCRVGTGLTDEELDAVTNKLRPYFRKSEYPKKTAPSFYQVTNNSKERPD 540
            PSNAYPRRFISFCRVGTGLTDE+LDAVTNKLRPYFRKSEYPKK APSFYQVTNNSKERPD
Sbjct: 481  PSNAYPRRFISFCRVGTGLTDEDLDAVTNKLRPYFRKSEYPKKAAPSFYQVTNNSKERPD 540

Query: 541  VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH 600
            VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH
Sbjct: 541  VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH 600

Query: 601  SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML 660
            SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML
Sbjct: 601  SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML 660

Query: 661  FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTKAGIKYEAAKRRGDVIHY 720
            FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTKAGIKYEAA+RRGDVIHY
Sbjct: 661  FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTKAGIKYEAARRRGDVIHY 720

Query: 721  SWILECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR 780
            SW+LECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR
Sbjct: 721  SWMLECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR 780

Query: 781  SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG 840
            SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG
Sbjct: 781  SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG 840

Query: 841  GKVSDNHVSATHVVVFCIPGSSVDCETVLKSFNEAEKHVILKNKIHIVAHQWLEDCLERA 900
            GKVSDNHVSATHVVVFCIPGSSVDCETVLK FNEAEKHVILKNKIHIVAHQWLEDCLERA
Sbjct: 841  GKVSDNHVSATHVVVFCIPGSSVDCETVLKCFNEAEKHVILKNKIHIVAHQWLEDCLERA 900

Query: 901  HRLQEDAYSLKPNWKMSIEKLSSDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ 960
            HRLQEDAYSLKPNWK SIEKL+SDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ
Sbjct: 901  HRLQEDAYSLKPNWKKSIEKLNSDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ 960

Query: 961  HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE 1020
            HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE
Sbjct: 961  HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE 1020

Query: 1021 KTNAETENIGTLCSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT 1080
            KTNAETENIGT CSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT
Sbjct: 1021 KTNAETENIGTPCSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT 1080

Query: 1081 ASARSMQKGKAGTEQGRRTQARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV 1140
            ASARSMQKGKAG EQG+RT+ARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV
Sbjct: 1081 ASARSMQKGKAGVEQGQRTRARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV 1140

Query: 1141 CPANSNRNEFIWPEDCDSSHKGKAIEQNDLAEFKSNVPEIEISTRYNCQGIEESEKLEVM 1200
            CPANSN NEFIWPE+CDSSHKGK IEQ+DLAEFKSNVPEIEISTRYNCQGIEESEKLEVM
Sbjct: 1141 CPANSNTNEFIWPENCDSSHKGKVIEQDDLAEFKSNVPEIEISTRYNCQGIEESEKLEVM 1200

Query: 1201 TDPLQAMLLDMVPSLGKSETKSSSTVLEEKQPVHNNSEPTKKKVSYKDVASELLKDW 1247
            TDPLQAMLLDMVPSLG SETKSSSTVLEEKQPV NNSEPTKKKVSYKDVASELLKDW
Sbjct: 1201 TDPLQAMLLDMVPSLGMSETKSSSTVLEEKQPVQNNSEPTKKKVSYKDVASELLKDW 1257

BLAST of MS007107 vs. NCBI nr
Match: XP_022149864.1 (DNA ligase 4 isoform X3 [Momordica charantia])

HSP 1 Score: 2399.4 bits (6217), Expect = 0.0e+00
Identity = 1219/1257 (96.98%), Postives = 1231/1257 (97.93%), Query Frame = 0

Query: 1    MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT 60
            MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT
Sbjct: 1    MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT 60

Query: 61   YGLKESVLATCLIDALGISRDSDDALRLTNWRKGGAKTGANAGNFALVAAEVLQRRQGMT 120
            YGLKESVLATCLIDALGISRDSDDALRL NWRKGGAKTGANAGNFALVAAEVLQRRQGMT
Sbjct: 61   YGLKESVLATCLIDALGISRDSDDALRLNNWRKGGAKTGANAGNFALVAAEVLQRRQGMT 120

Query: 121  PGGLTIRELNDLLDRLALSENSRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK 180
            PGGLTI+ELNDLLDRLALSEN RAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK
Sbjct: 121  PGGLTIKELNDLLDRLALSEN-RAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK 180

Query: 181  SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW 240
            SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW
Sbjct: 181  SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW 240

Query: 241  KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENILATRCIL 300
            KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENI+ATRCIL
Sbjct: 241  KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENIIATRCIL 300

Query: 301  DGEMLVWDKSLNRFAEFGSNQEIG-----------EWTDVAFDVLYVGDTSVIHRSLKER 360
            DGEMLVWDKSLNRFAEFGSNQEI            +   VAFDVLYVGDTSVIHRSLKER
Sbjct: 301  DGEMLVWDKSLNRFAEFGSNQEIARAAKDGLDSDRQLCYVAFDVLYVGDTSVIHRSLKER 360

Query: 361  HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV 420
            HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV
Sbjct: 361  HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV 420

Query: 421  IKDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS 480
            IKD+GSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS
Sbjct: 421  IKDMGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS 480

Query: 481  PSNAYPRRFISFCRVGTGLTDEELDAVTNKLRPYFRKSEYPKKTAPSFYQVTNNSKERPD 540
            PSNAYPRRFISFCRVGTGLTDE+LDAVTNKLRPYFRKSEYPKK APSFYQVTNNSKERPD
Sbjct: 481  PSNAYPRRFISFCRVGTGLTDEDLDAVTNKLRPYFRKSEYPKKAAPSFYQVTNNSKERPD 540

Query: 541  VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH 600
            VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH
Sbjct: 541  VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH 600

Query: 601  SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML 660
            SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML
Sbjct: 601  SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML 660

Query: 661  FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTKAGIKYEAAKRRGDVIHY 720
            FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTKAGIKYEAA+RRGDVIHY
Sbjct: 661  FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTKAGIKYEAARRRGDVIHY 720

Query: 721  SWILECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR 780
            SW+LECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR
Sbjct: 721  SWMLECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR 780

Query: 781  SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG 840
            SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG
Sbjct: 781  SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG 840

Query: 841  GKVSDNHVSATHVVVFCIPGSSVDCETVLKSFNEAEKHVILKNKIHIVAHQWLEDCLERA 900
            GKVSDNHVSATHVVVFCIPGSSVDCETVLK FNEAEKHVILKNKIHIVAHQWLEDCLERA
Sbjct: 841  GKVSDNHVSATHVVVFCIPGSSVDCETVLKCFNEAEKHVILKNKIHIVAHQWLEDCLERA 900

Query: 901  HRLQEDAYSLKPNWKMSIEKLSSDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ 960
            HRLQEDAYSLKPNWK SIEKL+SDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ
Sbjct: 901  HRLQEDAYSLKPNWKKSIEKLNSDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ 960

Query: 961  HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE 1020
            HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE
Sbjct: 961  HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE 1020

Query: 1021 KTNAETENIGTLCSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT 1080
            KTNAETENIGT CSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT
Sbjct: 1021 KTNAETENIGTPCSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT 1080

Query: 1081 ASARSMQKGKAGTEQGRRTQARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV 1140
            ASARSMQKGKAG EQG+RT+ARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV
Sbjct: 1081 ASARSMQKGKAGVEQGQRTRARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV 1140

Query: 1141 CPANSNRNEFIWPEDCDSSHKGKAIEQNDLAEFKSNVPEIEISTRYNCQGIEESEKLEVM 1200
            CPANSN NEFIWPE+CDSSHKGK IEQ+DLAEFKSNVPEIEISTRYNCQGIEESEKLEVM
Sbjct: 1141 CPANSNTNEFIWPENCDSSHKGKVIEQDDLAEFKSNVPEIEISTRYNCQGIEESEKLEVM 1200

Query: 1201 TDPLQAMLLDMVPSLGKSETKSSSTVLEEKQPVHNNSEPTKKKVSYKDVASELLKDW 1247
            TDPLQAMLLDMVPSLG SETKSSSTVLEEKQPV NNSEPTKKKVSYKDVASELLKDW
Sbjct: 1201 TDPLQAMLLDMVPSLGMSETKSSSTVLEEKQPVQNNSEPTKKKVSYKDVASELLKDW 1256

BLAST of MS007107 vs. NCBI nr
Match: XP_022149863.1 (DNA ligase 4 isoform X2 [Momordica charantia])

HSP 1 Score: 2399.4 bits (6217), Expect = 0.0e+00
Identity = 1219/1257 (96.98%), Postives = 1231/1257 (97.93%), Query Frame = 0

Query: 1    MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT 60
            MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT
Sbjct: 1    MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT 60

Query: 61   YGLKESVLATCLIDALGISRDSDDALRLTNWRKGGAKTGANAGNFALVAAEVLQRRQGMT 120
            YGLKESVLATCLIDALGISRDSDDALRL NWRKGGAKTGANAGNFALVAAEVLQRRQGMT
Sbjct: 61   YGLKESVLATCLIDALGISRDSDDALRLNNWRKGGAKTGANAGNFALVAAEVLQRRQGMT 120

Query: 121  PGGLTIRELNDLLDRLALSENSRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK 180
            PGGLTI+ELNDLLDRLALSENSRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK
Sbjct: 121  PGGLTIKELNDLLDRLALSENSRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK 180

Query: 181  SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW 240
            SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW
Sbjct: 181  SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW 240

Query: 241  KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENILATRCIL 300
            KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENI+ATRCIL
Sbjct: 241  KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENIIATRCIL 300

Query: 301  DGEMLVWDKSLNRFAEFGSNQEIG-----------EWTDVAFDVLYVGDTSVIHRSLKER 360
            DGEMLVWDKSLNRFAEFGSNQEI            +   VAFDVLYVGDTSVIHRSLKER
Sbjct: 301  DGEMLVWDKSLNRFAEFGSNQEIARAAKDGLDSDRQLCYVAFDVLYVGDTSVIHRSLKER 360

Query: 361  HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV 420
            HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV
Sbjct: 361  HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV 420

Query: 421  IKDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS 480
            IKD+GSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS
Sbjct: 421  IKDMGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS 480

Query: 481  PSNAYPRRFISFCRVGTGLTDEELDAVTNKLRPYFRKSEYPKKTAPSFYQVTNNSKERPD 540
            PSNAYPRRFISFCRVGTGLTDE+LDAVTNKLRPYFRKSEYPKK APSFYQVTNNSKERPD
Sbjct: 481  PSNAYPRRFISFCRVGTGLTDEDLDAVTNKLRPYFRKSEYPKKAAPSFYQVTNNSKERPD 540

Query: 541  VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH 600
            VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH
Sbjct: 541  VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH 600

Query: 601  SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML 660
            SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML
Sbjct: 601  SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML 660

Query: 661  FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTKAGIKYEAAKRRGDVIHY 720
            FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTK GIKYEAA+RRGDVIHY
Sbjct: 661  FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTK-GIKYEAARRRGDVIHY 720

Query: 721  SWILECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR 780
            SW+LECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR
Sbjct: 721  SWMLECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR 780

Query: 781  SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG 840
            SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG
Sbjct: 781  SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG 840

Query: 841  GKVSDNHVSATHVVVFCIPGSSVDCETVLKSFNEAEKHVILKNKIHIVAHQWLEDCLERA 900
            GKVSDNHVSATHVVVFCIPGSSVDCETVLK FNEAEKHVILKNKIHIVAHQWLEDCLERA
Sbjct: 841  GKVSDNHVSATHVVVFCIPGSSVDCETVLKCFNEAEKHVILKNKIHIVAHQWLEDCLERA 900

Query: 901  HRLQEDAYSLKPNWKMSIEKLSSDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ 960
            HRLQEDAYSLKPNWK SIEKL+SDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ
Sbjct: 901  HRLQEDAYSLKPNWKKSIEKLNSDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ 960

Query: 961  HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE 1020
            HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE
Sbjct: 961  HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE 1020

Query: 1021 KTNAETENIGTLCSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT 1080
            KTNAETENIGT CSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT
Sbjct: 1021 KTNAETENIGTPCSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT 1080

Query: 1081 ASARSMQKGKAGTEQGRRTQARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV 1140
            ASARSMQKGKAG EQG+RT+ARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV
Sbjct: 1081 ASARSMQKGKAGVEQGQRTRARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV 1140

Query: 1141 CPANSNRNEFIWPEDCDSSHKGKAIEQNDLAEFKSNVPEIEISTRYNCQGIEESEKLEVM 1200
            CPANSN NEFIWPE+CDSSHKGK IEQ+DLAEFKSNVPEIEISTRYNCQGIEESEKLEVM
Sbjct: 1141 CPANSNTNEFIWPENCDSSHKGKVIEQDDLAEFKSNVPEIEISTRYNCQGIEESEKLEVM 1200

Query: 1201 TDPLQAMLLDMVPSLGKSETKSSSTVLEEKQPVHNNSEPTKKKVSYKDVASELLKDW 1247
            TDPLQAMLLDMVPSLG SETKSSSTVLEEKQPV NNSEPTKKKVSYKDVASELLKDW
Sbjct: 1201 TDPLQAMLLDMVPSLGMSETKSSSTVLEEKQPVQNNSEPTKKKVSYKDVASELLKDW 1256

BLAST of MS007107 vs. NCBI nr
Match: XP_022149865.1 (DNA ligase 4 isoform X4 [Momordica charantia])

HSP 1 Score: 2393.2 bits (6201), Expect = 0.0e+00
Identity = 1218/1257 (96.90%), Postives = 1230/1257 (97.85%), Query Frame = 0

Query: 1    MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT 60
            MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT
Sbjct: 1    MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT 60

Query: 61   YGLKESVLATCLIDALGISRDSDDALRLTNWRKGGAKTGANAGNFALVAAEVLQRRQGMT 120
            YGLKESVLATCLIDALGISRDSDDALRL NWRKGGAKTGANAGNFALVAAEVLQRRQGMT
Sbjct: 61   YGLKESVLATCLIDALGISRDSDDALRLNNWRKGGAKTGANAGNFALVAAEVLQRRQGMT 120

Query: 121  PGGLTIRELNDLLDRLALSENSRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK 180
            PGGLTI+ELNDLLDRLALSEN RAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK
Sbjct: 121  PGGLTIKELNDLLDRLALSEN-RAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK 180

Query: 181  SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW 240
            SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW
Sbjct: 181  SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW 240

Query: 241  KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENILATRCIL 300
            KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENI+ATRCIL
Sbjct: 241  KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENIIATRCIL 300

Query: 301  DGEMLVWDKSLNRFAEFGSNQEIG-----------EWTDVAFDVLYVGDTSVIHRSLKER 360
            DGEMLVWDKSLNRFAEFGSNQEI            +   VAFDVLYVGDTSVIHRSLKER
Sbjct: 301  DGEMLVWDKSLNRFAEFGSNQEIARAAKDGLDSDRQLCYVAFDVLYVGDTSVIHRSLKER 360

Query: 361  HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV 420
            HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV
Sbjct: 361  HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV 420

Query: 421  IKDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS 480
            IKD+GSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS
Sbjct: 421  IKDMGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS 480

Query: 481  PSNAYPRRFISFCRVGTGLTDEELDAVTNKLRPYFRKSEYPKKTAPSFYQVTNNSKERPD 540
            PSNAYPRRFISFCRVGTGLTDE+LDAVTNKLRPYFRKSEYPKK APSFYQVTNNSKERPD
Sbjct: 481  PSNAYPRRFISFCRVGTGLTDEDLDAVTNKLRPYFRKSEYPKKAAPSFYQVTNNSKERPD 540

Query: 541  VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH 600
            VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH
Sbjct: 541  VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH 600

Query: 601  SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML 660
            SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML
Sbjct: 601  SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML 660

Query: 661  FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTKAGIKYEAAKRRGDVIHY 720
            FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTK GIKYEAA+RRGDVIHY
Sbjct: 661  FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTK-GIKYEAARRRGDVIHY 720

Query: 721  SWILECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR 780
            SW+LECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR
Sbjct: 721  SWMLECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR 780

Query: 781  SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG 840
            SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG
Sbjct: 781  SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG 840

Query: 841  GKVSDNHVSATHVVVFCIPGSSVDCETVLKSFNEAEKHVILKNKIHIVAHQWLEDCLERA 900
            GKVSDNHVSATHVVVFCIPGSSVDCETVLK FNEAEKHVILKNKIHIVAHQWLEDCLERA
Sbjct: 841  GKVSDNHVSATHVVVFCIPGSSVDCETVLKCFNEAEKHVILKNKIHIVAHQWLEDCLERA 900

Query: 901  HRLQEDAYSLKPNWKMSIEKLSSDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ 960
            HRLQEDAYSLKPNWK SIEKL+SDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ
Sbjct: 901  HRLQEDAYSLKPNWKKSIEKLNSDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ 960

Query: 961  HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE 1020
            HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE
Sbjct: 961  HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE 1020

Query: 1021 KTNAETENIGTLCSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT 1080
            KTNAETENIGT CSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT
Sbjct: 1021 KTNAETENIGTPCSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT 1080

Query: 1081 ASARSMQKGKAGTEQGRRTQARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV 1140
            ASARSMQKGKAG EQG+RT+ARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV
Sbjct: 1081 ASARSMQKGKAGVEQGQRTRARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV 1140

Query: 1141 CPANSNRNEFIWPEDCDSSHKGKAIEQNDLAEFKSNVPEIEISTRYNCQGIEESEKLEVM 1200
            CPANSN NEFIWPE+CDSSHKGK IEQ+DLAEFKSNVPEIEISTRYNCQGIEESEKLEVM
Sbjct: 1141 CPANSNTNEFIWPENCDSSHKGKVIEQDDLAEFKSNVPEIEISTRYNCQGIEESEKLEVM 1200

Query: 1201 TDPLQAMLLDMVPSLGKSETKSSSTVLEEKQPVHNNSEPTKKKVSYKDVASELLKDW 1247
            TDPLQAMLLDMVPSLG SETKSSSTVLEEKQPV NNSEPTKKKVSYKDVASELLKDW
Sbjct: 1201 TDPLQAMLLDMVPSLGMSETKSSSTVLEEKQPVQNNSEPTKKKVSYKDVASELLKDW 1255

BLAST of MS007107 vs. NCBI nr
Match: XP_022149866.1 (DNA ligase 4 isoform X5 [Momordica charantia])

HSP 1 Score: 2345.1 bits (6076), Expect = 0.0e+00
Identity = 1197/1257 (95.23%), Postives = 1208/1257 (96.10%), Query Frame = 0

Query: 1    MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT 60
            MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT
Sbjct: 1    MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT 60

Query: 61   YGLKESVLATCLIDALGISRDSDDALRLTNWRKGGAKTGANAGNFALVAAEVLQRRQGMT 120
            YGLKESVLATCLIDALGISRDSDDALRL NWRKGGAKTGANAGNFALVAAEVLQRRQGMT
Sbjct: 61   YGLKESVLATCLIDALGISRDSDDALRLNNWRKGGAKTGANAGNFALVAAEVLQRRQGMT 120

Query: 121  PGGLTIRELNDLLDRLALSENSRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK 180
            PGGLTI+ELNDLLDRLALSENSRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK
Sbjct: 121  PGGLTIKELNDLLDRLALSENSRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK 180

Query: 181  SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW 240
            SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW
Sbjct: 181  SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW 240

Query: 241  KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENILATRCIL 300
            KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFS                        RCIL
Sbjct: 241  KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFS------------------------RCIL 300

Query: 301  DGEMLVWDKSLNRFAEFGSNQEIG-----------EWTDVAFDVLYVGDTSVIHRSLKER 360
            DGEMLVWDKSLNRFAEFGSNQEI            +   VAFDVLYVGDTSVIHRSLKER
Sbjct: 301  DGEMLVWDKSLNRFAEFGSNQEIARAAKDGLDSDRQLCYVAFDVLYVGDTSVIHRSLKER 360

Query: 361  HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV 420
            HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV
Sbjct: 361  HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV 420

Query: 421  IKDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS 480
            IKD+GSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS
Sbjct: 421  IKDMGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS 480

Query: 481  PSNAYPRRFISFCRVGTGLTDEELDAVTNKLRPYFRKSEYPKKTAPSFYQVTNNSKERPD 540
            PSNAYPRRFISFCRVGTGLTDE+LDAVTNKLRPYFRKSEYPKK APSFYQVTNNSKERPD
Sbjct: 481  PSNAYPRRFISFCRVGTGLTDEDLDAVTNKLRPYFRKSEYPKKAAPSFYQVTNNSKERPD 540

Query: 541  VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH 600
            VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH
Sbjct: 541  VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH 600

Query: 601  SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML 660
            SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML
Sbjct: 601  SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML 660

Query: 661  FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTKAGIKYEAAKRRGDVIHY 720
            FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTKAGIKYEAA+RRGDVIHY
Sbjct: 661  FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTKAGIKYEAARRRGDVIHY 720

Query: 721  SWILECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR 780
            SW+LECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR
Sbjct: 721  SWMLECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR 780

Query: 781  SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG 840
            SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG
Sbjct: 781  SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG 840

Query: 841  GKVSDNHVSATHVVVFCIPGSSVDCETVLKSFNEAEKHVILKNKIHIVAHQWLEDCLERA 900
            GKVSDNHVSATHVVVFCIPGSSVDCETVLK FNEAEKHVILKNKIHIVAHQWLEDCLERA
Sbjct: 841  GKVSDNHVSATHVVVFCIPGSSVDCETVLKCFNEAEKHVILKNKIHIVAHQWLEDCLERA 900

Query: 901  HRLQEDAYSLKPNWKMSIEKLSSDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ 960
            HRLQEDAYSLKPNWK SIEKL+SDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ
Sbjct: 901  HRLQEDAYSLKPNWKKSIEKLNSDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ 960

Query: 961  HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE 1020
            HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE
Sbjct: 961  HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE 1020

Query: 1021 KTNAETENIGTLCSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT 1080
            KTNAETENIGT CSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT
Sbjct: 1021 KTNAETENIGTPCSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT 1080

Query: 1081 ASARSMQKGKAGTEQGRRTQARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV 1140
            ASARSMQKGKAG EQG+RT+ARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV
Sbjct: 1081 ASARSMQKGKAGVEQGQRTRARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV 1140

Query: 1141 CPANSNRNEFIWPEDCDSSHKGKAIEQNDLAEFKSNVPEIEISTRYNCQGIEESEKLEVM 1200
            CPANSN NEFIWPE+CDSSHKGK IEQ+DLAEFKSNVPEIEISTRYNCQGIEESEKLEVM
Sbjct: 1141 CPANSNTNEFIWPENCDSSHKGKVIEQDDLAEFKSNVPEIEISTRYNCQGIEESEKLEVM 1200

Query: 1201 TDPLQAMLLDMVPSLGKSETKSSSTVLEEKQPVHNNSEPTKKKVSYKDVASELLKDW 1247
            TDPLQAMLLDMVPSLG SETKSSSTVLEEKQPV NNSEPTKKKVSYKDVASELLKDW
Sbjct: 1201 TDPLQAMLLDMVPSLGMSETKSSSTVLEEKQPVQNNSEPTKKKVSYKDVASELLKDW 1233

BLAST of MS007107 vs. ExPASy Swiss-Prot
Match: Q9LL84 (DNA ligase 4 OS=Arabidopsis thaliana OX=3702 GN=LIG4 PE=1 SV=1)

HSP 1 Score: 1345.5 bits (3481), Expect = 0.0e+00
Identity = 731/1290 (56.67%), Postives = 910/1290 (70.54%), Query Frame = 0

Query: 1    MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT 60
            MTE I F+VL +LF+WIQ+SK+S++KRSKFRKFLDT+C  +DYF A+RLI+P+LDRERG+
Sbjct: 1    MTEEIKFSVLVSLFNWIQKSKTSSQKRSKFRKFLDTYCKPSDYFVAVRLIIPSLDRERGS 60

Query: 61   YGLKESVLATCLIDALGISRDSDDALRLTNWRKGG-AKTGANAGNFALVAAEVLQRRQGM 120
            YGLKESVLATCLIDALGISRD+ DA+RL NWRKGG AK GANAGNF+L+AAEVLQRRQGM
Sbjct: 61   YGLKESVLATCLIDALGISRDAPDAVRLLNWRKGGTAKAGANAGNFSLIAAEVLQRRQGM 120

Query: 121  TPGGLTIRELNDLLDRLALSENSRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSE 180
              GGLTI+ELNDLLDRLA SEN RAEKT VLSTLIQKTNAQEMKW++ IILKDLKLG+SE
Sbjct: 121  ASGGLTIKELNDLLDRLASSEN-RAEKTLVLSTLIQKTNAQEMKWVIRIILKDLKLGMSE 180

Query: 181  KSIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAA 240
            KSIF EFHPDAEDLFNVTCDLKLVCEKLRDR +RHKRQDIEVGKAVRPQLA R+ + NAA
Sbjct: 181  KSIFQEFHPDAEDLFNVTCDLKLVCEKLRDRHQRHKRQDIEVGKAVRPQLAMRIGDVNAA 240

Query: 241  WKKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENILATRCI 300
            WKKLHGK+VV ECKFDGDRIQIHKNGT+IH+FSRNFLDHSEY   MSD+IV+NIL  +CI
Sbjct: 241  WKKLHGKDVVAECKFDGDRIQIHKNGTDIHYFSRNFLDHSEYAHAMSDLIVQNILVDKCI 300

Query: 301  LDGEMLVWDKSLNRFAEFGSNQEIG----EWTD-------VAFDVLYVGDTSVIHRSLKE 360
            LDGEMLVWD SLNRFAEFGSNQEI     E  D       VAFDVLYVGDTSVIH+SLKE
Sbjct: 301  LDGEMLVWDTSLNRFAEFGSNQEIAKAAREGLDSHKQLCYVAFDVLYVGDTSVIHQSLKE 360

Query: 361  RHELLRDVVKPVKGRLEILVPNGGLN-SDCAGDPSWSQRAYNADDVERFFKNTIENRDEG 420
            RHELL+ VVKP+KGRLE+LVP GGLN    +G+PSWS   + A DVERFFK T+ENRDEG
Sbjct: 361  RHELLKKVVKPLKGRLEVLVPEGGLNVHRPSGEPSWSIVVHAAADVERFFKETVENRDEG 420

Query: 421  IVIKDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAE 480
            IV+KDL SKWEPGDRSGKW+KLKP+Y+RAG+DLD LIIGGYYGSGRRGGEVAQFL+ LA+
Sbjct: 421  IVLKDLESKWEPGDRSGKWMKLKPEYIRAGADLDVLIIGGYYGSGRRGGEVAQFLVALAD 480

Query: 481  RSPSNAYPRRFISFCRVGTGLTDEELDAVTNKLRPYFRKSEYPKKTAPSFYQVTNNSKER 540
            R+ +N YPRRF+SFCRVGTGL+D+EL+ V +KL+PYFRK+E+PKK  PSFYQVTN+SKER
Sbjct: 481  RAEANVYPRRFMSFCRVGTGLSDDELNTVVSKLKPYFRKNEHPKKAPPSFYQVTNHSKER 540

Query: 541  PDVWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVEL 600
            PDVWI+SPEKSIILSITSDIRTI+SEVF APYSLRFPRID+VRYDKPWHECLDVQ+FVEL
Sbjct: 541  PDVWIDSPEKSIILSITSDIRTIRSEVFVAPYSLRFPRIDKVRYDKPWHECLDVQAFVEL 600

Query: 601  VHSSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSN 660
            V+SSNG TQ+  ++   QD N K  KS+ +  KK+VS+VPS  +QTD+S IK  + IFSN
Sbjct: 601  VNSSNGTTQKQKESESTQD-NPKVNKSSKRGEKKNVSLVPSQFIQTDVSDIKGKTSIFSN 660

Query: 661  MLFYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTKAGIKYEAAKRRGDVI 720
            M+FY VNVP +HSL++ H+++VENGG FSMNLN+SVTH +AA++ +GIKY+AAKR+ DVI
Sbjct: 661  MIFYFVNVPRSHSLETFHKMVVENGGKFSMNLNNSVTHCIAAES-SGIKYQAAKRQRDVI 720

Query: 721  HYSWILECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNV 780
            H+SW+L+CCS+ K+LPL PK+FL L+D S+ KL+++ID FSDSY+WDL+L  LKQ+LSN 
Sbjct: 721  HFSWVLDCCSRNKMLPLLPKYFLHLTDASRTKLQDDIDEFSDSYYWDLDLEGLKQVLSNA 780

Query: 781  SRSEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSF 840
             +SED K+IDYY+ K CP+  WS  +   +YF P   +L  + + LL +  +RL +EV  
Sbjct: 781  KQSEDSKSIDYYKKKLCPEKRWSCLLSCCVYFYPYSQTLSTEEEALLGIMAKRLMLEVLM 840

Query: 841  RGGKVSDNHVSATHVVVFCIPGSSVDCETVLKSFNEAEKHVILKNKIHIVAHQWLEDCLE 900
             GGKVS+N   A+H+VV  +    +D   V KSF+E EK ++LK ++H+V+  WLE+ L+
Sbjct: 841  AGGKVSNNLAHASHLVVLAMAEEPLDFTLVSKSFSEMEKRLLLKKRLHVVSSHWLEESLQ 900

Query: 901  RAHRLQEDAYSLKPNWKMSIEKLSSDIDSETPLASENTENQKISSPSNAYKDQGRNEAAS 960
            R  +L ED Y+L+P       K   + D+E    SE+   +  S  S   K     E AS
Sbjct: 901  REEKLCEDVYTLRP-------KYMEESDTEESDKSEHDTTEVASQGSAQTK-----EPAS 960

Query: 961  GQHMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYN 1020
             +  +  S        RG+S  R++++G+ S   ++R + R  K P+KIS  E++ SD +
Sbjct: 961  SKIAITSS--------RGRSNTRAVKRGRSSTNSLQRVQRRRGKQPSKISGDETEESDAS 1020

Query: 1021 DEKTNAETENIGTLCSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPT 1080
            +EK +    +I                                   T+  GE +R     
Sbjct: 1021 EEKVSTRLSDIAE--------------------------------ETDSFGEAQRN---- 1080

Query: 1081 PTASARSMQKGKAGTEQGRRTQ-ARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNI 1140
             ++  +  ++GK+   Q +R Q +R  K +AKIGG ESD +D  D   N   +  E    
Sbjct: 1081 -SSRGKCAKRGKSRVGQTQRVQRSRRGKKAAKIGGDESDENDELDGNNNVSADAEEGNAA 1140

Query: 1141 GTVCPANSNRNEFIWPEDCDSSHKGKAIEQNDLAEFKSNVPEIEISTRYNCQGIEESEKL 1200
            G        R   I               +  L + ++   E+++            EKL
Sbjct: 1141 GRSVENEETREPDIAKYTESQQRDNTVAVEEALQDSRNAKTEMDM-----------KEKL 1200

Query: 1201 EVMTDPLQAMLLDM--VPSLGKSETKSSSTVLEEKQPVHNNSEPT--------------- 1247
            ++  DPLQAML+ M  +PS   +ET + +T    K  V    E +               
Sbjct: 1201 QIHEDPLQAMLMKMFPIPSQKTTETSNRTTGEYRKANVSGECESSEKRKLDAETDNTSVN 1219

BLAST of MS007107 vs. ExPASy Swiss-Prot
Match: Q7X7E9 (Putative DNA ligase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=LIG4 PE=2 SV=2)

HSP 1 Score: 1037.7 bits (2682), Expect = 1.1e-301
Identity = 633/1433 (44.17%), Postives = 832/1433 (58.06%), Query Frame = 0

Query: 1    MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSA---DYFSAIRLILPTLDRE 60
            M   + F +L  +F  + R +++AKKR++ R  LD         DYFSA+RL+LP LDRE
Sbjct: 1    MAATVRFGLLVAMFQAMSRDRTAAKKRARLRALLDRAYGPGGRDDYFSALRLVLPGLDRE 60

Query: 61   RGTYGLKESVLATCLIDALGISRDSDDALRLTNWRKGGAKTGANAGNFALVAAEVLQRRQ 120
            RG+YGLKE+ LA+ L+DALGI++DS DA+RLTNWR+GG     NAGNFALVAAEVLQRRQ
Sbjct: 61   RGSYGLKEAALASVLVDALGIAKDSPDAVRLTNWRRGGG--FRNAGNFALVAAEVLQRRQ 120

Query: 121  GMTPGGLTIRELNDLLDRLALSENS-------------RAEKTAVLSTLIQKTNAQEMKW 180
            GMT GGLTI+E+ND LDRLA +ENS             R+EK ++LS+LI+KTNA EMKW
Sbjct: 121  GMTSGGLTIKEVNDALDRLAATENSVCLESEKLLLILDRSEKASILSSLIKKTNALEMKW 180

Query: 181  IVMIILKDLKLGVSEKSIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKA 240
            ++MIILKDLKLG+SEKS+FHEFHPDAEDLFNVTCDL+LVCEKL DR +RHKRQDIEVGKA
Sbjct: 181  LLMIILKDLKLGISEKSVFHEFHPDAEDLFNVTCDLRLVCEKLNDRSQRHKRQDIEVGKA 240

Query: 241  VRPQLASRVSNANAAWKKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRG 300
            VRPQL+ RV+NA++AWKKLHGK+VV ECKFDGDRIQIHKNG EIHFFSR+FLDHSEY  G
Sbjct: 241  VRPQLSMRVNNASSAWKKLHGKQVVAECKFDGDRIQIHKNGEEIHFFSRSFLDHSEYAPG 300

Query: 301  MSDVIVENILATRCILDGEMLVWDKSLNRFAEFGSNQEIG-----------------EW- 360
            MS VI+ENIL  RCILDGEMLVWD  LNRFAEFGSNQEI                  EW 
Sbjct: 301  MSKVIIENILVDRCILDGEMLVWDTVLNRFAEFGSNQEIAKAAKEGLETDRQLCCILEWI 360

Query: 361  ------------------------TDVAFDVLYVGDTSVIHRSLKERHELLRDVVKPVKG 420
                                    + VAFD+LY GDTSVIH+SL ERHE+L+ VV+P+KG
Sbjct: 361  ASYVLALCVNILSCQDFFFSNKRESYVAFDILYAGDTSVIHQSLTERHEILQKVVRPLKG 420

Query: 421  RLEILVPNGGLNSDCAGD-PSWSQRAYNADDVERFFKNTIEN------------------ 480
             LEILVP GGLN     D P WS  A++ DDVE+FFK+T++N                  
Sbjct: 421  HLEILVPTGGLNIHRPPDEPCWSILAHSLDDVEKFFKDTVDNRFFPTSPNTDELSNLSIS 480

Query: 481  ----------------RDEGIVIKDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGG 540
                            R+EGI++KDL SKWEPGDRSGKWLKLKPDY+ AG+DLD +IIGG
Sbjct: 481  LMIQFRFSDYSICYVLREEGIILKDLESKWEPGDRSGKWLKLKPDYIHAGADLDVIIIGG 540

Query: 541  YYGSGRRGGEVAQFLMGLAERSPSNAYPRRFISFCRVGTGLTDEELDAVTNKLRPYFRKS 600
            YYGSGRRGGEVAQFL+GLA  S  N+YP+RF+SFCRVGTGL+DEELDA+  KL+P+FRK+
Sbjct: 541  YYGSGRRGGEVAQFLVGLAVPSDDNSYPKRFLSFCRVGTGLSDEELDALVTKLKPHFRKN 600

Query: 601  EYPKKTAPSFYQVTNNSKERPDVWIESPEK-----------------SIILSITSDIRTI 660
            EYPKK  P FY+VTN+SKERPDVWIESP+K                 S+I+SITSDIRTI
Sbjct: 601  EYPKK-PPRFYEVTNHSKERPDVWIESPDKQSVRFRKHLIYCITKCRSVIISITSDIRTI 660

Query: 661  KSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVHSSNGNTQRGTDNSGWQDSNRK 720
            KSEVFAAPYSLRFPRI R+RYDKPWHECLDVQ+FV++VHSSNG T R  D+         
Sbjct: 661  KSEVFAAPYSLRFPRIQRLRYDKPWHECLDVQAFVDIVHSSNGTTHRAADD--------- 720

Query: 721  FIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNMLFYIVNVPPTHSLDSLHRLIVE 780
                                   D+  +K                               
Sbjct: 721  ---------------------DNDLKNVK------------------------------- 780

Query: 781  NGGTFSMNLNDSVTHSVAADTKAGIKYEAAKRRGDVIHYSWILECCSQKKLLPLQPKHFL 840
            NGG+FSMNLNDSVTH +AA+ K       A R+G +IHYSWIL+CC +K+LL LQPK   
Sbjct: 781  NGGSFSMNLNDSVTHCIAAEKK-------ATRQGRIIHYSWILDCCKEKRLLHLQPKFDP 840

Query: 841  FLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSRS-EDVKAIDYYRGKYCPKDEW 900
             L      K  EEID+++D ++WD++++DLKQ+ SN+ R+  D   + +Y+ KYC  + +
Sbjct: 841  CL--QPMHKFPEEIDSYADYFYWDIDISDLKQIFSNMDRAVVDSNMVHHYKKKYCADERF 900

Query: 901  SIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRGGKVSDNHVSATHVVVFCIPG 960
              F G  +YF    P +  D++V+ +L+++R+K +++  GG+V      ATH+++  +  
Sbjct: 901  CFFQGCCVYFYH-APLVNADYNVISDLALKRVKQDLTMHGGQVCSILAPATHLIIVSVL- 960

Query: 961  SSVDCETVLKSFNEAEKHVILKNKIHIVAHQWLEDCLERAHRLQEDAYSLKPNWKMSIEK 1020
             + + + + KS   AE+  +   ++ +V+++WLED +E+  RL E  YSLKP+   ++E+
Sbjct: 961  QAYNFDMLYKSLPPAERRYLHDKRLQVVSNKWLEDSVEKQTRLPETTYSLKPD---TLEE 1020

Query: 1021 LSSDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQHMLLHSTERSGGKKRGKSTA 1080
            +  +   ET              P N   ++  NE A   H+      +   +KRG+ ++
Sbjct: 1021 IEIERSEET------------VQPCNDKLEE--NEKADTSHV------KHAPRKRGRPSS 1080

Query: 1081 RSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYND---EKTNAETENIGTLCSENL 1140
             + +  K +   VRR RAR     AKI +VE + SD+ +   +    +T+NI  +  ++ 
Sbjct: 1081 SASRTAKPAPRPVRRTRARRGNQHAKIDDVEPEESDHGETGLDDQIPDTDNISKMEVDSF 1140

Query: 1141 KTNELT-KLENFDPSRKGKGIEHVPLCSTERT--GEKKRGRRPTPTASARSMQKGKAGTE 1200
              ++++ +      +R+GK    +    +E +  GE  +  +         M++  +  +
Sbjct: 1141 DKDQVSARPVRRTRARRGKQHAKIDYGQSEESDPGETGQDDQRLDADYISKMEEDSSDRD 1200

Query: 1201 QG---------RRTQARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTVCPANS 1247
            QG         RR++A+  K  AKI           DDK         K N  ++    S
Sbjct: 1201 QGAHPTAPRVVRRSRAQRGKWLAKIDRETGPGETGQDDK---------KLNADSI----S 1260

BLAST of MS007107 vs. ExPASy Swiss-Prot
Match: Q5R6L3 (DNA ligase 4 OS=Pongo abelii OX=9601 GN=DNL4 PE=2 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 5.3e-112
Identity = 288/936 (30.77%), Postives = 473/936 (50.53%), Query Frame = 0

Query: 5   INFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSAD------------YFSAIRLILP 64
           + F  L +  + IQ+SK  A+K   FR+FLD++    D            ++ A+RLILP
Sbjct: 14  VPFADLCSTLERIQKSKGRAEKIRHFREFLDSWRKFHDAFHKNQKDVTDSFYPAMRLILP 73

Query: 65  TLDRERGTYGLKESVLATCLIDALGISRDSDDALRLTNWRKGGAKTGANAGNFALVAAEV 124
            L+RER  YG+KE++LA   I+ L + RD  DAL+L N+R     T  +AG+FA++A  V
Sbjct: 74  QLERERMAYGIKETMLAKLYIELLNLPRDGKDALKLLNYRT-PTGTHGDAGDFAMIAYFV 133

Query: 125 LQRRQGMTPGGLTIRELNDLLDRLALSENSRAEKTAVLSTLIQ---KTNAQEMKWIVMII 184
           L+ R  +  G LTI+++NDLLD +A S NS   K  +  +L+Q   +++A E KW++ +I
Sbjct: 134 LKPR-CLQKGSLTIQQVNDLLDSIA-SNNSAKRKDLIKKSLLQLITQSSALEQKWLIRMI 193

Query: 185 LKDLKLGVSEKSIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQL 244
           +KDLKLGVS+++IF  FH DA +L NVT DL+ VC +L D         I +  A +P L
Sbjct: 194 IKDLKLGVSQQTIFSVFHSDAVELHNVTTDLEKVCRQLHDPSVGLSDISITLFSAFKPML 253

Query: 245 ASRVSNANAAWKKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHS-EYG----RG 304
           A+ +++     K +  +   +E K DG+R+Q+HK+G    +FSRN  +++ ++G     G
Sbjct: 254 AA-IADIEHIEKDMKHQSFYIETKLDGERMQMHKDGDVYKYFSRNGYNYTDQFGASPTEG 313

Query: 305 MSDVIVENILATR---CILDGEMLVWDKSLNRFAEFGSNQEIGEWTD--------VAFDV 364
                + N   T    CILDGEM+ ++ +   F + G+  +I    +          FDV
Sbjct: 314 SLTPFIHNAFKTDIQICILDGEMMAYNPNTQTFMQKGTKFDIKRMVEDSDLQTCYCVFDV 373

Query: 365 LYVGDTSVIHRSLKERHELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDV 424
           L V +  + H +L++R+E+L  +  P+ GR+EI+                  +A+  ++V
Sbjct: 374 LMVNNKKLGHETLRKRYEILSSIFTPIPGRIEIV---------------QKTQAHTKNEV 433

Query: 425 ERFFKNTIENRDEGIVIKDLGSKWEPGDRSGKWLKLKPDYVRAGSD-LDALIIGGYYGSG 484
                  I+ R+EGI+IK   S ++P  R   WLK+KP+YV    D LD LI+GGY+G G
Sbjct: 434 IDALNEAIDKREEGIMIKQPLSIYKPDKRGEGWLKIKPEYVSGLMDELDILIVGGYWGKG 493

Query: 485 RRGGEVAQFLMGLAERSPSNAYPRRFISFCRVGTGLTDEELDAVTNKLRPYFRKSEYPKK 544
            RGG ++ FL  +AE+ P    P  F +  RVG+G T +EL  +  KL  Y++   + KK
Sbjct: 494 SRGGMMSHFLCAVAEKPPPGEKPSVFHTLSRVGSGCTMKELYDLGLKLAKYWK--PFHKK 553

Query: 545 TAPSFYQVTNNSKERPDVWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYD 604
             PS         E+P+V+IE P  S+I+ I +    + S+++    +LRFPRI+++R D
Sbjct: 554 APPSSILC---GTEKPEVYIE-PCNSVIVQIKA-AEIVPSDMYKTGCTLRFPRIEKIRDD 613

Query: 605 KPWHECLDVQSFVELVHSSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQ 664
           K WHEC+ +    +L   ++G         G  D  ++  +    + KK + ++  HL  
Sbjct: 614 KEWHECMTLDDLEQLRGKASGKLASKHFYVGGDDEPQEKKRKAAPKMKKVIGII-EHLKA 673

Query: 665 TDISGIKEDSLIFSNMLFYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTK 724
            +++ + + S IF ++ F +++   +     L   I E GG    N        +A    
Sbjct: 674 PNLTNVNKISNIFEDVEFCVMSGTDSQPKPDLENRIAEFGGYIVQNPGPDTYCVIAGSKN 733

Query: 725 AGIKYEAAKRRGDVIHYSWILECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYF 784
             +K      + DV+  +W+LEC   K  +P QP   + +   +K+    E D + DSYF
Sbjct: 734 IRVKNIILSNKHDVVKPAWLLECFKTKSFVPWQPHFMIHMCPSTKEHFAREYDCYGDSYF 793

Query: 785 WDLELADLKQLLSNVSRSED---------VKAIDYYRGKYCPKDEWSIFVGFQIYFLPLR 844
            D +L  LK++ S +  S +         +  ++Y     C     S+F    +Y L L 
Sbjct: 794 VDTDLNQLKEVFSGIKNSNEQTPEEMASLIADLEYRYSWDC--SPLSMFRRHTVY-LDLY 853

Query: 845 PSLKFDWDVLLELSMRRLK-VEVSFRGGK-VSDNHVSATHVVVFCIPGSSVDCETVLKSF 898
             +  D     E +   +K +E+ F G K VS      +HV++        D +   ++F
Sbjct: 854 AVIN-DLSTKNEGTRLAIKALELRFHGAKVVSCLAEGVSHVIIGEDHSRIADFKAFRRTF 909

BLAST of MS007107 vs. ExPASy Swiss-Prot
Match: P49917 (DNA ligase 4 OS=Homo sapiens OX=9606 GN=LIG4 PE=1 SV=2)

HSP 1 Score: 404.4 bits (1038), Expect = 4.5e-111
Identity = 286/942 (30.36%), Postives = 473/942 (50.21%), Query Frame = 0

Query: 5   INFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSAD------------YFSAIRLILP 64
           + F  L +  + IQ+SK  A+K   FR+FLD++    D            ++ A+RLILP
Sbjct: 14  VPFADLCSTLERIQKSKGRAEKIRHFREFLDSWRKFHDALHKNHKDVTDSFYPAMRLILP 73

Query: 65  TLDRERGTYGLKESVLATCLIDALGISRDSDDALRLTNWRKGGAKTGANAGNFALVAAEV 124
            L+RER  YG+KE++LA   I+ L + RD  DAL+L N+R     T  +AG+FA++A  V
Sbjct: 74  QLERERMAYGIKETMLAKLYIELLNLPRDGKDALKLLNYRT-PTGTHGDAGDFAMIAYFV 133

Query: 125 LQRRQGMTPGGLTIRELNDLLDRLALSENSRAEKTAVLSTLIQ---KTNAQEMKWIVMII 184
           L+ R  +  G LTI+++NDLLD +A S NS   K  +  +L+Q   +++A E KW++ +I
Sbjct: 134 LKPR-CLQKGSLTIQQVNDLLDSIA-SNNSAKRKDLIKKSLLQLITQSSALEQKWLIRMI 193

Query: 185 LKDLKLGVSEKSIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQL 244
           +KDLKLGVS+++IF  FH DA +L NVT DL+ VC +L D         I +  A +P L
Sbjct: 194 IKDLKLGVSQQTIFSVFHNDAAELHNVTTDLEKVCRQLHDPSVGLSDISITLFSAFKPML 253

Query: 245 ASRVSNANAAWKKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHS-EYG----RG 304
           A+ +++     K +  +   +E K DG+R+Q+HK+G    +FSRN  +++ ++G     G
Sbjct: 254 AA-IADIEHIEKDMKHQSFYIETKLDGERMQMHKDGDVYKYFSRNGYNYTDQFGASPTEG 313

Query: 305 MSDVIVENILATR---CILDGEMLVWDKSLNRFAEFGSNQEIGEWTD--------VAFDV 364
                + N        CILDGEM+ ++ +   F + G+  +I    +          FDV
Sbjct: 314 SLTPFIHNAFKADIQICILDGEMMAYNPNTQTFMQKGTKFDIKRMVEDSDLQTCYCVFDV 373

Query: 365 LYVGDTSVIHRSLKERHELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDV 424
           L V +  + H +L++R+E+L  +  P+ GR+EI+                  +A+  ++V
Sbjct: 374 LMVNNKKLGHETLRKRYEILSSIFTPIPGRIEIV---------------QKTQAHTKNEV 433

Query: 425 ERFFKNTIENRDEGIVIKDLGSKWEPGDRSGKWLKLKPDYVRAGSD-LDALIIGGYYGSG 484
                  I+ R+EGI++K   S ++P  R   WLK+KP+YV    D LD LI+GGY+G G
Sbjct: 434 IDALNEAIDKREEGIMVKQPLSIYKPDKRGEGWLKIKPEYVSGLMDELDILIVGGYWGKG 493

Query: 485 RRGGEVAQFLMGLAERSPSNAYPRRFISFCRVGTGLTDEELDAVTNKLRPYFRKSEYPKK 544
            RGG ++ FL  +AE+ P    P  F +  RVG+G T +EL  +  KL  Y++   + +K
Sbjct: 494 SRGGMMSHFLCAVAEKPPPGEKPSVFHTLSRVGSGCTMKELYDLGLKLAKYWK--PFHRK 553

Query: 545 TAPSFYQVTNNSKERPDVWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYD 604
             PS         E+P+V+IE P  S+I+ I +    + S+++    +LRFPRI+++R D
Sbjct: 554 APPSSILC---GTEKPEVYIE-PCNSVIVQIKA-AEIVPSDMYKTGCTLRFPRIEKIRDD 613

Query: 605 KPWHECLDVQSFVELVHSSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQ 664
           K WHEC+ +    +L   ++G         G  D  ++  +    + KK + ++  HL  
Sbjct: 614 KEWHECMTLDDLEQLRGKASGKLASKHLYIGGDDEPQEKKRKAAPKMKKVIGII-EHLKA 673

Query: 665 TDISGIKEDSLIFSNMLFYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTK 724
            +++ + + S IF ++ F +++   +     L   I E GG    N        +A    
Sbjct: 674 PNLTNVNKISNIFEDVEFCVMSGTDSQPKPDLENRIAEFGGYIVQNPGPDTYCVIAGSEN 733

Query: 725 AGIKYEAAKRRGDVIHYSWILECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYF 784
             +K      + DV+  +W+LEC   K  +P QP+  + +   +K+    E D + DSYF
Sbjct: 734 IRVKNIILSNKHDVVKPAWLLECFKTKSFVPWQPRFMIHMCPSTKEHFAREYDCYGDSYF 793

Query: 785 WDLELADLKQLLSNVSRSED---------VKAIDYYRGKYCPKDEWSIFVGFQIYFLPLR 844
            D +L  LK++ S +  S +         +  ++Y     C     S+F    +Y     
Sbjct: 794 IDTDLNQLKEVFSGIKNSNEQTPEEMASLIADLEYRYSWDC--SPLSMFRRHTVYL---- 853

Query: 845 PSLKFDWDVLLELSMR----RLKV---EVSFRGGK-VSDNHVSATHVVVFCIPGSSVDCE 898
                 + V+ +LS +    RL +   E+ F G K VS      +HV++    G      
Sbjct: 854 ----DSYAVINDLSTKNEGTRLAIKALELRFHGAKVVSCLAEGVSHVII----GEDHSRV 909

BLAST of MS007107 vs. ExPASy Swiss-Prot
Match: Q8BTF7 (DNA ligase 4 OS=Mus musculus OX=10090 GN=Lig4 PE=2 SV=2)

HSP 1 Score: 404.1 bits (1037), Expect = 5.9e-111
Identity = 291/939 (30.99%), Postives = 476/939 (50.69%), Query Frame = 0

Query: 5   INFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSAD------------YFSAIRLILP 64
           + F  L +  + IQ+ K  A+K   F++FLD++    D            ++ A+RLILP
Sbjct: 14  VPFADLCSTLERIQKGKDRAEKIRHFKEFLDSWRKFHDALHKNRKDVTDSFYPAMRLILP 73

Query: 65  TLDRERGTYGLKESVLATCLIDALGISRDSDDALRLTNWR-KGGAKTGANAGNFALVAAE 124
            L+RER  YG+KE++LA   I+ L + R+  DA +L N+R   GA+T  +AG+FA++A  
Sbjct: 74  QLERERMAYGIKETMLAKLYIELLNLPREGKDAQKLLNYRTPSGART--DAGDFAMIAYF 133

Query: 125 VLQRRQGMTPGGLTIRELNDLLDRLALSENSRAEKTAVLSTLIQ---KTNAQEMKWIVMI 184
           VL+ R  +  G LTI+++N+LLD L  S NS  +K  V  +L+Q   +++A E KW++ +
Sbjct: 134 VLKPR-CLQKGSLTIQQVNELLD-LVASNNSGKKKDLVKKSLLQLITQSSALEQKWLIRM 193

Query: 185 ILKDLKLGVSEKSIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQ 244
           I+KDLKLG+S+++IF  FH DA +L NVT DL+ VC +L D         I +  A +P 
Sbjct: 194 IIKDLKLGISQQTIFSIFHNDAVELHNVTTDLEKVCRQLHDPSVGLSDISITLFSAFKPM 253

Query: 245 LASRVSNANAAWKKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHS-EYG----R 304
           LA+ V++     K +  +   +E K DG+R+Q+HK+G    +FSRN  +++ ++G     
Sbjct: 254 LAA-VADVERVEKDMKQQSFYIETKLDGERMQMHKDGALYRYFSRNGYNYTDQFGESPQE 313

Query: 305 GMSDVIVENILAT---RCILDGEMLVWDKSLNRFAEFGSNQEIGEWTD--------VAFD 364
           G     + N   T    CILDGEM+ ++ +   F + G   +I    +          FD
Sbjct: 314 GSLTPFIHNAFGTDVQACILDGEMMAYNPTTQTFMQKGVKFDIKRMVEDSGLQTCYSVFD 373

Query: 365 VLYVGDTSVIHRSLKERHELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADD 424
           VL V    +   +L++R+E+L     P++GR+EI+                  +A+   +
Sbjct: 374 VLMVNKKKLGRETLRKRYEILSSTFTPIQGRIEIV---------------QKTQAHTKKE 433

Query: 425 VERFFKNTIENRDEGIVIKDLGSKWEPGDRSGKWLKLKPDYVRAGSD-LDALIIGGYYGS 484
           V     + I+ R+EGI++K   S ++P  R   WLK+KP+YV    D LD LI+GGY+G 
Sbjct: 434 VVDALNDAIDKREEGIMVKHPLSIYKPDKRGEGWLKIKPEYVSGLMDELDVLIVGGYWGK 493

Query: 485 GRRGGEVAQFLMGLAERSPSNAYPRRFISFCRVGTGLTDEELDAVTNKLRPYFRKSEYPK 544
           G RGG ++ FL  +AE  P    P  F + CRVG+G T +EL  +  KL  Y++   + K
Sbjct: 494 GSRGGMMSHFLCAVAETPPPGDRPSVFHTLCRVGSGYTMKELYDLGLKLAKYWK--PFHK 553

Query: 545 KTAPSFYQVTNNSKERPDVWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRY 604
           K+ PS         E+P+V+IE P+ S+I+ I +    + S+++    +LRFPRI+++R 
Sbjct: 554 KSPPSSILC---GTEKPEVYIE-PQNSVIVQIKA-AEIVPSDMYKTGSTLRFPRIEKIRD 613

Query: 605 DKPWHECLDVQSFVELVHSSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLL 664
           DK WHEC+ +    +L   ++G       + G  D  R+  +    + KK++ ++  HL 
Sbjct: 614 DKEWHECMTLGDLEQLRGKASGKLATKHLHVGDDDEPREKRRKPISKTKKAIRII-EHLK 673

Query: 665 QTDISGIKEDSLIFSNMLFYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADT 724
             ++S + + S +F ++ F +++    +    L   I E GG    N        +A   
Sbjct: 674 APNLSNVNKVSNVFEDVEFCVMSGLDGYPKADLENRIAEFGGYIVQNPGPDTYCVIAGSE 733

Query: 725 KAGIKYEAAKRRGDVIHYSWILECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSY 784
              +K   +  + DV+   W+LEC   K  +P QP+  + +   +K+    E D + DSY
Sbjct: 734 NVRVKNIISSDKNDVVKPEWLLECFKTKTCVPWQPRFMIHMCPSTKQHFAREYDCYGDSY 793

Query: 785 FWDLELADLKQLLSNVSRSED------VKAIDYYRGKYC-PKDEWSIFVGFQIYFLPLRP 844
           F D +L  LK++   +  SE          I     +Y       S+F  + IY L L  
Sbjct: 794 FVDTDLDQLKEVFLGIKPSEQQTPEEMAPVIADLECRYSWDHSPLSMFRHYTIY-LDLYA 853

Query: 845 SLKFDWDVLLELSMRRLKV---EVSFRGGK-VSDNHVSATHVVVFCIPGSSVDCETVLKS 900
            +    D+   +   RL +   E+ F G K VS      +HV++    G      T  K 
Sbjct: 854 VIN---DLSSRIEATRLGITALELRFHGAKVVSCLSEGVSHVII----GEDQRRVTDFKI 911

BLAST of MS007107 vs. ExPASy TrEMBL
Match: A0A6J1D959 (DNA ligase OS=Momordica charantia OX=3673 GN=LOC111018188 PE=3 SV=1)

HSP 1 Score: 2405.6 bits (6233), Expect = 0.0e+00
Identity = 1220/1257 (97.06%), Postives = 1232/1257 (98.01%), Query Frame = 0

Query: 1    MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT 60
            MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT
Sbjct: 1    MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT 60

Query: 61   YGLKESVLATCLIDALGISRDSDDALRLTNWRKGGAKTGANAGNFALVAAEVLQRRQGMT 120
            YGLKESVLATCLIDALGISRDSDDALRL NWRKGGAKTGANAGNFALVAAEVLQRRQGMT
Sbjct: 61   YGLKESVLATCLIDALGISRDSDDALRLNNWRKGGAKTGANAGNFALVAAEVLQRRQGMT 120

Query: 121  PGGLTIRELNDLLDRLALSENSRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK 180
            PGGLTI+ELNDLLDRLALSENSRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK
Sbjct: 121  PGGLTIKELNDLLDRLALSENSRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK 180

Query: 181  SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW 240
            SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW
Sbjct: 181  SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW 240

Query: 241  KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENILATRCIL 300
            KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENI+ATRCIL
Sbjct: 241  KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENIIATRCIL 300

Query: 301  DGEMLVWDKSLNRFAEFGSNQEIG-----------EWTDVAFDVLYVGDTSVIHRSLKER 360
            DGEMLVWDKSLNRFAEFGSNQEI            +   VAFDVLYVGDTSVIHRSLKER
Sbjct: 301  DGEMLVWDKSLNRFAEFGSNQEIARAAKDGLDSDRQLCYVAFDVLYVGDTSVIHRSLKER 360

Query: 361  HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV 420
            HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV
Sbjct: 361  HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV 420

Query: 421  IKDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS 480
            IKD+GSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS
Sbjct: 421  IKDMGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS 480

Query: 481  PSNAYPRRFISFCRVGTGLTDEELDAVTNKLRPYFRKSEYPKKTAPSFYQVTNNSKERPD 540
            PSNAYPRRFISFCRVGTGLTDE+LDAVTNKLRPYFRKSEYPKK APSFYQVTNNSKERPD
Sbjct: 481  PSNAYPRRFISFCRVGTGLTDEDLDAVTNKLRPYFRKSEYPKKAAPSFYQVTNNSKERPD 540

Query: 541  VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH 600
            VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH
Sbjct: 541  VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH 600

Query: 601  SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML 660
            SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML
Sbjct: 601  SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML 660

Query: 661  FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTKAGIKYEAAKRRGDVIHY 720
            FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTKAGIKYEAA+RRGDVIHY
Sbjct: 661  FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTKAGIKYEAARRRGDVIHY 720

Query: 721  SWILECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR 780
            SW+LECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR
Sbjct: 721  SWMLECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR 780

Query: 781  SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG 840
            SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG
Sbjct: 781  SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG 840

Query: 841  GKVSDNHVSATHVVVFCIPGSSVDCETVLKSFNEAEKHVILKNKIHIVAHQWLEDCLERA 900
            GKVSDNHVSATHVVVFCIPGSSVDCETVLK FNEAEKHVILKNKIHIVAHQWLEDCLERA
Sbjct: 841  GKVSDNHVSATHVVVFCIPGSSVDCETVLKCFNEAEKHVILKNKIHIVAHQWLEDCLERA 900

Query: 901  HRLQEDAYSLKPNWKMSIEKLSSDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ 960
            HRLQEDAYSLKPNWK SIEKL+SDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ
Sbjct: 901  HRLQEDAYSLKPNWKKSIEKLNSDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ 960

Query: 961  HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE 1020
            HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE
Sbjct: 961  HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE 1020

Query: 1021 KTNAETENIGTLCSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT 1080
            KTNAETENIGT CSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT
Sbjct: 1021 KTNAETENIGTPCSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT 1080

Query: 1081 ASARSMQKGKAGTEQGRRTQARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV 1140
            ASARSMQKGKAG EQG+RT+ARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV
Sbjct: 1081 ASARSMQKGKAGVEQGQRTRARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV 1140

Query: 1141 CPANSNRNEFIWPEDCDSSHKGKAIEQNDLAEFKSNVPEIEISTRYNCQGIEESEKLEVM 1200
            CPANSN NEFIWPE+CDSSHKGK IEQ+DLAEFKSNVPEIEISTRYNCQGIEESEKLEVM
Sbjct: 1141 CPANSNTNEFIWPENCDSSHKGKVIEQDDLAEFKSNVPEIEISTRYNCQGIEESEKLEVM 1200

Query: 1201 TDPLQAMLLDMVPSLGKSETKSSSTVLEEKQPVHNNSEPTKKKVSYKDVASELLKDW 1247
            TDPLQAMLLDMVPSLG SETKSSSTVLEEKQPV NNSEPTKKKVSYKDVASELLKDW
Sbjct: 1201 TDPLQAMLLDMVPSLGMSETKSSSTVLEEKQPVQNNSEPTKKKVSYKDVASELLKDW 1257

BLAST of MS007107 vs. ExPASy TrEMBL
Match: A0A6J1D7W8 (DNA ligase OS=Momordica charantia OX=3673 GN=LOC111018188 PE=3 SV=1)

HSP 1 Score: 2399.4 bits (6217), Expect = 0.0e+00
Identity = 1219/1257 (96.98%), Postives = 1231/1257 (97.93%), Query Frame = 0

Query: 1    MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT 60
            MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT
Sbjct: 1    MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT 60

Query: 61   YGLKESVLATCLIDALGISRDSDDALRLTNWRKGGAKTGANAGNFALVAAEVLQRRQGMT 120
            YGLKESVLATCLIDALGISRDSDDALRL NWRKGGAKTGANAGNFALVAAEVLQRRQGMT
Sbjct: 61   YGLKESVLATCLIDALGISRDSDDALRLNNWRKGGAKTGANAGNFALVAAEVLQRRQGMT 120

Query: 121  PGGLTIRELNDLLDRLALSENSRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK 180
            PGGLTI+ELNDLLDRLALSENSRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK
Sbjct: 121  PGGLTIKELNDLLDRLALSENSRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK 180

Query: 181  SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW 240
            SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW
Sbjct: 181  SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW 240

Query: 241  KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENILATRCIL 300
            KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENI+ATRCIL
Sbjct: 241  KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENIIATRCIL 300

Query: 301  DGEMLVWDKSLNRFAEFGSNQEIG-----------EWTDVAFDVLYVGDTSVIHRSLKER 360
            DGEMLVWDKSLNRFAEFGSNQEI            +   VAFDVLYVGDTSVIHRSLKER
Sbjct: 301  DGEMLVWDKSLNRFAEFGSNQEIARAAKDGLDSDRQLCYVAFDVLYVGDTSVIHRSLKER 360

Query: 361  HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV 420
            HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV
Sbjct: 361  HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV 420

Query: 421  IKDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS 480
            IKD+GSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS
Sbjct: 421  IKDMGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS 480

Query: 481  PSNAYPRRFISFCRVGTGLTDEELDAVTNKLRPYFRKSEYPKKTAPSFYQVTNNSKERPD 540
            PSNAYPRRFISFCRVGTGLTDE+LDAVTNKLRPYFRKSEYPKK APSFYQVTNNSKERPD
Sbjct: 481  PSNAYPRRFISFCRVGTGLTDEDLDAVTNKLRPYFRKSEYPKKAAPSFYQVTNNSKERPD 540

Query: 541  VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH 600
            VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH
Sbjct: 541  VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH 600

Query: 601  SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML 660
            SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML
Sbjct: 601  SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML 660

Query: 661  FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTKAGIKYEAAKRRGDVIHY 720
            FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTK GIKYEAA+RRGDVIHY
Sbjct: 661  FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTK-GIKYEAARRRGDVIHY 720

Query: 721  SWILECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR 780
            SW+LECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR
Sbjct: 721  SWMLECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR 780

Query: 781  SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG 840
            SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG
Sbjct: 781  SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG 840

Query: 841  GKVSDNHVSATHVVVFCIPGSSVDCETVLKSFNEAEKHVILKNKIHIVAHQWLEDCLERA 900
            GKVSDNHVSATHVVVFCIPGSSVDCETVLK FNEAEKHVILKNKIHIVAHQWLEDCLERA
Sbjct: 841  GKVSDNHVSATHVVVFCIPGSSVDCETVLKCFNEAEKHVILKNKIHIVAHQWLEDCLERA 900

Query: 901  HRLQEDAYSLKPNWKMSIEKLSSDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ 960
            HRLQEDAYSLKPNWK SIEKL+SDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ
Sbjct: 901  HRLQEDAYSLKPNWKKSIEKLNSDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ 960

Query: 961  HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE 1020
            HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE
Sbjct: 961  HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE 1020

Query: 1021 KTNAETENIGTLCSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT 1080
            KTNAETENIGT CSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT
Sbjct: 1021 KTNAETENIGTPCSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT 1080

Query: 1081 ASARSMQKGKAGTEQGRRTQARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV 1140
            ASARSMQKGKAG EQG+RT+ARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV
Sbjct: 1081 ASARSMQKGKAGVEQGQRTRARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV 1140

Query: 1141 CPANSNRNEFIWPEDCDSSHKGKAIEQNDLAEFKSNVPEIEISTRYNCQGIEESEKLEVM 1200
            CPANSN NEFIWPE+CDSSHKGK IEQ+DLAEFKSNVPEIEISTRYNCQGIEESEKLEVM
Sbjct: 1141 CPANSNTNEFIWPENCDSSHKGKVIEQDDLAEFKSNVPEIEISTRYNCQGIEESEKLEVM 1200

Query: 1201 TDPLQAMLLDMVPSLGKSETKSSSTVLEEKQPVHNNSEPTKKKVSYKDVASELLKDW 1247
            TDPLQAMLLDMVPSLG SETKSSSTVLEEKQPV NNSEPTKKKVSYKDVASELLKDW
Sbjct: 1201 TDPLQAMLLDMVPSLGMSETKSSSTVLEEKQPVQNNSEPTKKKVSYKDVASELLKDW 1256

BLAST of MS007107 vs. ExPASy TrEMBL
Match: A0A6J1D8A6 (DNA ligase OS=Momordica charantia OX=3673 GN=LOC111018188 PE=3 SV=1)

HSP 1 Score: 2399.4 bits (6217), Expect = 0.0e+00
Identity = 1219/1257 (96.98%), Postives = 1231/1257 (97.93%), Query Frame = 0

Query: 1    MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT 60
            MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT
Sbjct: 1    MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT 60

Query: 61   YGLKESVLATCLIDALGISRDSDDALRLTNWRKGGAKTGANAGNFALVAAEVLQRRQGMT 120
            YGLKESVLATCLIDALGISRDSDDALRL NWRKGGAKTGANAGNFALVAAEVLQRRQGMT
Sbjct: 61   YGLKESVLATCLIDALGISRDSDDALRLNNWRKGGAKTGANAGNFALVAAEVLQRRQGMT 120

Query: 121  PGGLTIRELNDLLDRLALSENSRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK 180
            PGGLTI+ELNDLLDRLALSEN RAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK
Sbjct: 121  PGGLTIKELNDLLDRLALSEN-RAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK 180

Query: 181  SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW 240
            SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW
Sbjct: 181  SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW 240

Query: 241  KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENILATRCIL 300
            KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENI+ATRCIL
Sbjct: 241  KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENIIATRCIL 300

Query: 301  DGEMLVWDKSLNRFAEFGSNQEIG-----------EWTDVAFDVLYVGDTSVIHRSLKER 360
            DGEMLVWDKSLNRFAEFGSNQEI            +   VAFDVLYVGDTSVIHRSLKER
Sbjct: 301  DGEMLVWDKSLNRFAEFGSNQEIARAAKDGLDSDRQLCYVAFDVLYVGDTSVIHRSLKER 360

Query: 361  HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV 420
            HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV
Sbjct: 361  HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV 420

Query: 421  IKDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS 480
            IKD+GSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS
Sbjct: 421  IKDMGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS 480

Query: 481  PSNAYPRRFISFCRVGTGLTDEELDAVTNKLRPYFRKSEYPKKTAPSFYQVTNNSKERPD 540
            PSNAYPRRFISFCRVGTGLTDE+LDAVTNKLRPYFRKSEYPKK APSFYQVTNNSKERPD
Sbjct: 481  PSNAYPRRFISFCRVGTGLTDEDLDAVTNKLRPYFRKSEYPKKAAPSFYQVTNNSKERPD 540

Query: 541  VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH 600
            VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH
Sbjct: 541  VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH 600

Query: 601  SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML 660
            SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML
Sbjct: 601  SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML 660

Query: 661  FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTKAGIKYEAAKRRGDVIHY 720
            FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTKAGIKYEAA+RRGDVIHY
Sbjct: 661  FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTKAGIKYEAARRRGDVIHY 720

Query: 721  SWILECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR 780
            SW+LECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR
Sbjct: 721  SWMLECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR 780

Query: 781  SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG 840
            SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG
Sbjct: 781  SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG 840

Query: 841  GKVSDNHVSATHVVVFCIPGSSVDCETVLKSFNEAEKHVILKNKIHIVAHQWLEDCLERA 900
            GKVSDNHVSATHVVVFCIPGSSVDCETVLK FNEAEKHVILKNKIHIVAHQWLEDCLERA
Sbjct: 841  GKVSDNHVSATHVVVFCIPGSSVDCETVLKCFNEAEKHVILKNKIHIVAHQWLEDCLERA 900

Query: 901  HRLQEDAYSLKPNWKMSIEKLSSDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ 960
            HRLQEDAYSLKPNWK SIEKL+SDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ
Sbjct: 901  HRLQEDAYSLKPNWKKSIEKLNSDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ 960

Query: 961  HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE 1020
            HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE
Sbjct: 961  HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE 1020

Query: 1021 KTNAETENIGTLCSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT 1080
            KTNAETENIGT CSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT
Sbjct: 1021 KTNAETENIGTPCSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT 1080

Query: 1081 ASARSMQKGKAGTEQGRRTQARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV 1140
            ASARSMQKGKAG EQG+RT+ARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV
Sbjct: 1081 ASARSMQKGKAGVEQGQRTRARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV 1140

Query: 1141 CPANSNRNEFIWPEDCDSSHKGKAIEQNDLAEFKSNVPEIEISTRYNCQGIEESEKLEVM 1200
            CPANSN NEFIWPE+CDSSHKGK IEQ+DLAEFKSNVPEIEISTRYNCQGIEESEKLEVM
Sbjct: 1141 CPANSNTNEFIWPENCDSSHKGKVIEQDDLAEFKSNVPEIEISTRYNCQGIEESEKLEVM 1200

Query: 1201 TDPLQAMLLDMVPSLGKSETKSSSTVLEEKQPVHNNSEPTKKKVSYKDVASELLKDW 1247
            TDPLQAMLLDMVPSLG SETKSSSTVLEEKQPV NNSEPTKKKVSYKDVASELLKDW
Sbjct: 1201 TDPLQAMLLDMVPSLGMSETKSSSTVLEEKQPVQNNSEPTKKKVSYKDVASELLKDW 1256

BLAST of MS007107 vs. ExPASy TrEMBL
Match: A0A6J1D6X8 (DNA ligase OS=Momordica charantia OX=3673 GN=LOC111018188 PE=3 SV=1)

HSP 1 Score: 2393.2 bits (6201), Expect = 0.0e+00
Identity = 1218/1257 (96.90%), Postives = 1230/1257 (97.85%), Query Frame = 0

Query: 1    MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT 60
            MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT
Sbjct: 1    MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT 60

Query: 61   YGLKESVLATCLIDALGISRDSDDALRLTNWRKGGAKTGANAGNFALVAAEVLQRRQGMT 120
            YGLKESVLATCLIDALGISRDSDDALRL NWRKGGAKTGANAGNFALVAAEVLQRRQGMT
Sbjct: 61   YGLKESVLATCLIDALGISRDSDDALRLNNWRKGGAKTGANAGNFALVAAEVLQRRQGMT 120

Query: 121  PGGLTIRELNDLLDRLALSENSRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK 180
            PGGLTI+ELNDLLDRLALSEN RAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK
Sbjct: 121  PGGLTIKELNDLLDRLALSEN-RAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK 180

Query: 181  SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW 240
            SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW
Sbjct: 181  SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW 240

Query: 241  KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENILATRCIL 300
            KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENI+ATRCIL
Sbjct: 241  KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENIIATRCIL 300

Query: 301  DGEMLVWDKSLNRFAEFGSNQEIG-----------EWTDVAFDVLYVGDTSVIHRSLKER 360
            DGEMLVWDKSLNRFAEFGSNQEI            +   VAFDVLYVGDTSVIHRSLKER
Sbjct: 301  DGEMLVWDKSLNRFAEFGSNQEIARAAKDGLDSDRQLCYVAFDVLYVGDTSVIHRSLKER 360

Query: 361  HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV 420
            HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV
Sbjct: 361  HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV 420

Query: 421  IKDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS 480
            IKD+GSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS
Sbjct: 421  IKDMGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS 480

Query: 481  PSNAYPRRFISFCRVGTGLTDEELDAVTNKLRPYFRKSEYPKKTAPSFYQVTNNSKERPD 540
            PSNAYPRRFISFCRVGTGLTDE+LDAVTNKLRPYFRKSEYPKK APSFYQVTNNSKERPD
Sbjct: 481  PSNAYPRRFISFCRVGTGLTDEDLDAVTNKLRPYFRKSEYPKKAAPSFYQVTNNSKERPD 540

Query: 541  VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH 600
            VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH
Sbjct: 541  VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH 600

Query: 601  SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML 660
            SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML
Sbjct: 601  SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML 660

Query: 661  FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTKAGIKYEAAKRRGDVIHY 720
            FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTK GIKYEAA+RRGDVIHY
Sbjct: 661  FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTK-GIKYEAARRRGDVIHY 720

Query: 721  SWILECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR 780
            SW+LECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR
Sbjct: 721  SWMLECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR 780

Query: 781  SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG 840
            SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG
Sbjct: 781  SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG 840

Query: 841  GKVSDNHVSATHVVVFCIPGSSVDCETVLKSFNEAEKHVILKNKIHIVAHQWLEDCLERA 900
            GKVSDNHVSATHVVVFCIPGSSVDCETVLK FNEAEKHVILKNKIHIVAHQWLEDCLERA
Sbjct: 841  GKVSDNHVSATHVVVFCIPGSSVDCETVLKCFNEAEKHVILKNKIHIVAHQWLEDCLERA 900

Query: 901  HRLQEDAYSLKPNWKMSIEKLSSDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ 960
            HRLQEDAYSLKPNWK SIEKL+SDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ
Sbjct: 901  HRLQEDAYSLKPNWKKSIEKLNSDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ 960

Query: 961  HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE 1020
            HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE
Sbjct: 961  HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE 1020

Query: 1021 KTNAETENIGTLCSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT 1080
            KTNAETENIGT CSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT
Sbjct: 1021 KTNAETENIGTPCSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT 1080

Query: 1081 ASARSMQKGKAGTEQGRRTQARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV 1140
            ASARSMQKGKAG EQG+RT+ARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV
Sbjct: 1081 ASARSMQKGKAGVEQGQRTRARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV 1140

Query: 1141 CPANSNRNEFIWPEDCDSSHKGKAIEQNDLAEFKSNVPEIEISTRYNCQGIEESEKLEVM 1200
            CPANSN NEFIWPE+CDSSHKGK IEQ+DLAEFKSNVPEIEISTRYNCQGIEESEKLEVM
Sbjct: 1141 CPANSNTNEFIWPENCDSSHKGKVIEQDDLAEFKSNVPEIEISTRYNCQGIEESEKLEVM 1200

Query: 1201 TDPLQAMLLDMVPSLGKSETKSSSTVLEEKQPVHNNSEPTKKKVSYKDVASELLKDW 1247
            TDPLQAMLLDMVPSLG SETKSSSTVLEEKQPV NNSEPTKKKVSYKDVASELLKDW
Sbjct: 1201 TDPLQAMLLDMVPSLGMSETKSSSTVLEEKQPVQNNSEPTKKKVSYKDVASELLKDW 1255

BLAST of MS007107 vs. ExPASy TrEMBL
Match: A0A6J1D9Q4 (DNA ligase IV OS=Momordica charantia OX=3673 GN=LOC111018188 PE=3 SV=1)

HSP 1 Score: 2345.1 bits (6076), Expect = 0.0e+00
Identity = 1197/1257 (95.23%), Postives = 1208/1257 (96.10%), Query Frame = 0

Query: 1    MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT 60
            MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT
Sbjct: 1    MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT 60

Query: 61   YGLKESVLATCLIDALGISRDSDDALRLTNWRKGGAKTGANAGNFALVAAEVLQRRQGMT 120
            YGLKESVLATCLIDALGISRDSDDALRL NWRKGGAKTGANAGNFALVAAEVLQRRQGMT
Sbjct: 61   YGLKESVLATCLIDALGISRDSDDALRLNNWRKGGAKTGANAGNFALVAAEVLQRRQGMT 120

Query: 121  PGGLTIRELNDLLDRLALSENSRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK 180
            PGGLTI+ELNDLLDRLALSENSRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK
Sbjct: 121  PGGLTIKELNDLLDRLALSENSRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSEK 180

Query: 181  SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW 240
            SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW
Sbjct: 181  SIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAAW 240

Query: 241  KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENILATRCIL 300
            KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFS                        RCIL
Sbjct: 241  KKLHGKEVVVECKFDGDRIQIHKNGTEIHFFS------------------------RCIL 300

Query: 301  DGEMLVWDKSLNRFAEFGSNQEIG-----------EWTDVAFDVLYVGDTSVIHRSLKER 360
            DGEMLVWDKSLNRFAEFGSNQEI            +   VAFDVLYVGDTSVIHRSLKER
Sbjct: 301  DGEMLVWDKSLNRFAEFGSNQEIARAAKDGLDSDRQLCYVAFDVLYVGDTSVIHRSLKER 360

Query: 361  HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV 420
            HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV
Sbjct: 361  HELLRDVVKPVKGRLEILVPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIV 420

Query: 421  IKDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS 480
            IKD+GSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS
Sbjct: 421  IKDMGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAERS 480

Query: 481  PSNAYPRRFISFCRVGTGLTDEELDAVTNKLRPYFRKSEYPKKTAPSFYQVTNNSKERPD 540
            PSNAYPRRFISFCRVGTGLTDE+LDAVTNKLRPYFRKSEYPKK APSFYQVTNNSKERPD
Sbjct: 481  PSNAYPRRFISFCRVGTGLTDEDLDAVTNKLRPYFRKSEYPKKAAPSFYQVTNNSKERPD 540

Query: 541  VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH 600
            VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH
Sbjct: 541  VWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVELVH 600

Query: 601  SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML 660
            SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML
Sbjct: 601  SSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSNML 660

Query: 661  FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTKAGIKYEAAKRRGDVIHY 720
            FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTKAGIKYEAA+RRGDVIHY
Sbjct: 661  FYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTKAGIKYEAARRRGDVIHY 720

Query: 721  SWILECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR 780
            SW+LECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR
Sbjct: 721  SWMLECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNVSR 780

Query: 781  SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG 840
            SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG
Sbjct: 781  SEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSFRG 840

Query: 841  GKVSDNHVSATHVVVFCIPGSSVDCETVLKSFNEAEKHVILKNKIHIVAHQWLEDCLERA 900
            GKVSDNHVSATHVVVFCIPGSSVDCETVLK FNEAEKHVILKNKIHIVAHQWLEDCLERA
Sbjct: 841  GKVSDNHVSATHVVVFCIPGSSVDCETVLKCFNEAEKHVILKNKIHIVAHQWLEDCLERA 900

Query: 901  HRLQEDAYSLKPNWKMSIEKLSSDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ 960
            HRLQEDAYSLKPNWK SIEKL+SDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ
Sbjct: 901  HRLQEDAYSLKPNWKKSIEKLNSDIDSETPLASENTENQKISSPSNAYKDQGRNEAASGQ 960

Query: 961  HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE 1020
            HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE
Sbjct: 961  HMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDE 1020

Query: 1021 KTNAETENIGTLCSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT 1080
            KTNAETENIGT CSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT
Sbjct: 1021 KTNAETENIGTPCSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPT 1080

Query: 1081 ASARSMQKGKAGTEQGRRTQARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV 1140
            ASARSMQKGKAG EQG+RT+ARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV
Sbjct: 1081 ASARSMQKGKAGVEQGQRTRARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTV 1140

Query: 1141 CPANSNRNEFIWPEDCDSSHKGKAIEQNDLAEFKSNVPEIEISTRYNCQGIEESEKLEVM 1200
            CPANSN NEFIWPE+CDSSHKGK IEQ+DLAEFKSNVPEIEISTRYNCQGIEESEKLEVM
Sbjct: 1141 CPANSNTNEFIWPENCDSSHKGKVIEQDDLAEFKSNVPEIEISTRYNCQGIEESEKLEVM 1200

Query: 1201 TDPLQAMLLDMVPSLGKSETKSSSTVLEEKQPVHNNSEPTKKKVSYKDVASELLKDW 1247
            TDPLQAMLLDMVPSLG SETKSSSTVLEEKQPV NNSEPTKKKVSYKDVASELLKDW
Sbjct: 1201 TDPLQAMLLDMVPSLGMSETKSSSTVLEEKQPVQNNSEPTKKKVSYKDVASELLKDW 1233

BLAST of MS007107 vs. TAIR 10
Match: AT5G57160.1 (DNA ligase IV )

HSP 1 Score: 1345.5 bits (3481), Expect = 0.0e+00
Identity = 731/1290 (56.67%), Postives = 910/1290 (70.54%), Query Frame = 0

Query: 1    MTELINFNVLSTLFDWIQRSKSSAKKRSKFRKFLDTFCSSADYFSAIRLILPTLDRERGT 60
            MTE I F+VL +LF+WIQ+SK+S++KRSKFRKFLDT+C  +DYF A+RLI+P+LDRERG+
Sbjct: 1    MTEEIKFSVLVSLFNWIQKSKTSSQKRSKFRKFLDTYCKPSDYFVAVRLIIPSLDRERGS 60

Query: 61   YGLKESVLATCLIDALGISRDSDDALRLTNWRKGG-AKTGANAGNFALVAAEVLQRRQGM 120
            YGLKESVLATCLIDALGISRD+ DA+RL NWRKGG AK GANAGNF+L+AAEVLQRRQGM
Sbjct: 61   YGLKESVLATCLIDALGISRDAPDAVRLLNWRKGGTAKAGANAGNFSLIAAEVLQRRQGM 120

Query: 121  TPGGLTIRELNDLLDRLALSENSRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVSE 180
              GGLTI+ELNDLLDRLA SEN RAEKT VLSTLIQKTNAQEMKW++ IILKDLKLG+SE
Sbjct: 121  ASGGLTIKELNDLLDRLASSEN-RAEKTLVLSTLIQKTNAQEMKWVIRIILKDLKLGMSE 180

Query: 181  KSIFHEFHPDAEDLFNVTCDLKLVCEKLRDRRKRHKRQDIEVGKAVRPQLASRVSNANAA 240
            KSIF EFHPDAEDLFNVTCDLKLVCEKLRDR +RHKRQDIEVGKAVRPQLA R+ + NAA
Sbjct: 181  KSIFQEFHPDAEDLFNVTCDLKLVCEKLRDRHQRHKRQDIEVGKAVRPQLAMRIGDVNAA 240

Query: 241  WKKLHGKEVVVECKFDGDRIQIHKNGTEIHFFSRNFLDHSEYGRGMSDVIVENILATRCI 300
            WKKLHGK+VV ECKFDGDRIQIHKNGT+IH+FSRNFLDHSEY   MSD+IV+NIL  +CI
Sbjct: 241  WKKLHGKDVVAECKFDGDRIQIHKNGTDIHYFSRNFLDHSEYAHAMSDLIVQNILVDKCI 300

Query: 301  LDGEMLVWDKSLNRFAEFGSNQEIG----EWTD-------VAFDVLYVGDTSVIHRSLKE 360
            LDGEMLVWD SLNRFAEFGSNQEI     E  D       VAFDVLYVGDTSVIH+SLKE
Sbjct: 301  LDGEMLVWDTSLNRFAEFGSNQEIAKAAREGLDSHKQLCYVAFDVLYVGDTSVIHQSLKE 360

Query: 361  RHELLRDVVKPVKGRLEILVPNGGLN-SDCAGDPSWSQRAYNADDVERFFKNTIENRDEG 420
            RHELL+ VVKP+KGRLE+LVP GGLN    +G+PSWS   + A DVERFFK T+ENRDEG
Sbjct: 361  RHELLKKVVKPLKGRLEVLVPEGGLNVHRPSGEPSWSIVVHAAADVERFFKETVENRDEG 420

Query: 421  IVIKDLGSKWEPGDRSGKWLKLKPDYVRAGSDLDALIIGGYYGSGRRGGEVAQFLMGLAE 480
            IV+KDL SKWEPGDRSGKW+KLKP+Y+RAG+DLD LIIGGYYGSGRRGGEVAQFL+ LA+
Sbjct: 421  IVLKDLESKWEPGDRSGKWMKLKPEYIRAGADLDVLIIGGYYGSGRRGGEVAQFLVALAD 480

Query: 481  RSPSNAYPRRFISFCRVGTGLTDEELDAVTNKLRPYFRKSEYPKKTAPSFYQVTNNSKER 540
            R+ +N YPRRF+SFCRVGTGL+D+EL+ V +KL+PYFRK+E+PKK  PSFYQVTN+SKER
Sbjct: 481  RAEANVYPRRFMSFCRVGTGLSDDELNTVVSKLKPYFRKNEHPKKAPPSFYQVTNHSKER 540

Query: 541  PDVWIESPEKSIILSITSDIRTIKSEVFAAPYSLRFPRIDRVRYDKPWHECLDVQSFVEL 600
            PDVWI+SPEKSIILSITSDIRTI+SEVF APYSLRFPRID+VRYDKPWHECLDVQ+FVEL
Sbjct: 541  PDVWIDSPEKSIILSITSDIRTIRSEVFVAPYSLRFPRIDKVRYDKPWHECLDVQAFVEL 600

Query: 601  VHSSNGNTQRGTDNSGWQDSNRKFIKSTGKRRKKSVSVVPSHLLQTDISGIKEDSLIFSN 660
            V+SSNG TQ+  ++   QD N K  KS+ +  KK+VS+VPS  +QTD+S IK  + IFSN
Sbjct: 601  VNSSNGTTQKQKESESTQD-NPKVNKSSKRGEKKNVSLVPSQFIQTDVSDIKGKTSIFSN 660

Query: 661  MLFYIVNVPPTHSLDSLHRLIVENGGTFSMNLNDSVTHSVAADTKAGIKYEAAKRRGDVI 720
            M+FY VNVP +HSL++ H+++VENGG FSMNLN+SVTH +AA++ +GIKY+AAKR+ DVI
Sbjct: 661  MIFYFVNVPRSHSLETFHKMVVENGGKFSMNLNNSVTHCIAAES-SGIKYQAAKRQRDVI 720

Query: 721  HYSWILECCSQKKLLPLQPKHFLFLSDHSKKKLEEEIDAFSDSYFWDLELADLKQLLSNV 780
            H+SW+L+CCS+ K+LPL PK+FL L+D S+ KL+++ID FSDSY+WDL+L  LKQ+LSN 
Sbjct: 721  HFSWVLDCCSRNKMLPLLPKYFLHLTDASRTKLQDDIDEFSDSYYWDLDLEGLKQVLSNA 780

Query: 781  SRSEDVKAIDYYRGKYCPKDEWSIFVGFQIYFLPLRPSLKFDWDVLLELSMRRLKVEVSF 840
             +SED K+IDYY+ K CP+  WS  +   +YF P   +L  + + LL +  +RL +EV  
Sbjct: 781  KQSEDSKSIDYYKKKLCPEKRWSCLLSCCVYFYPYSQTLSTEEEALLGIMAKRLMLEVLM 840

Query: 841  RGGKVSDNHVSATHVVVFCIPGSSVDCETVLKSFNEAEKHVILKNKIHIVAHQWLEDCLE 900
             GGKVS+N   A+H+VV  +    +D   V KSF+E EK ++LK ++H+V+  WLE+ L+
Sbjct: 841  AGGKVSNNLAHASHLVVLAMAEEPLDFTLVSKSFSEMEKRLLLKKRLHVVSSHWLEESLQ 900

Query: 901  RAHRLQEDAYSLKPNWKMSIEKLSSDIDSETPLASENTENQKISSPSNAYKDQGRNEAAS 960
            R  +L ED Y+L+P       K   + D+E    SE+   +  S  S   K     E AS
Sbjct: 901  REEKLCEDVYTLRP-------KYMEESDTEESDKSEHDTTEVASQGSAQTK-----EPAS 960

Query: 961  GQHMLLHSTERSGGKKRGKSTARSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYN 1020
             +  +  S        RG+S  R++++G+ S   ++R + R  K P+KIS  E++ SD +
Sbjct: 961  SKIAITSS--------RGRSNTRAVKRGRSSTNSLQRVQRRRGKQPSKISGDETEESDAS 1020

Query: 1021 DEKTNAETENIGTLCSENLKTNELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPT 1080
            +EK +    +I                                   T+  GE +R     
Sbjct: 1021 EEKVSTRLSDIAE--------------------------------ETDSFGEAQRN---- 1080

Query: 1081 PTASARSMQKGKAGTEQGRRTQ-ARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNI 1140
             ++  +  ++GK+   Q +R Q +R  K +AKIGG ESD +D  D   N   +  E    
Sbjct: 1081 -SSRGKCAKRGKSRVGQTQRVQRSRRGKKAAKIGGDESDENDELDGNNNVSADAEEGNAA 1140

Query: 1141 GTVCPANSNRNEFIWPEDCDSSHKGKAIEQNDLAEFKSNVPEIEISTRYNCQGIEESEKL 1200
            G        R   I               +  L + ++   E+++            EKL
Sbjct: 1141 GRSVENEETREPDIAKYTESQQRDNTVAVEEALQDSRNAKTEMDM-----------KEKL 1200

Query: 1201 EVMTDPLQAMLLDM--VPSLGKSETKSSSTVLEEKQPVHNNSEPT--------------- 1247
            ++  DPLQAML+ M  +PS   +ET + +T    K  V    E +               
Sbjct: 1201 QIHEDPLQAMLMKMFPIPSQKTTETSNRTTGEYRKANVSGECESSEKRKLDAETDNTSVN 1219

BLAST of MS007107 vs. TAIR 10
Match: AT1G08130.1 (DNA ligase 1 )

HSP 1 Score: 110.9 bits (276), Expect = 7.3e-24
Identity = 141/597 (23.62%), Postives = 242/597 (40.54%), Query Frame = 0

Query: 62  GLKESVLATCLIDALGISRDSDDALRLTNWRKGGAKTGANAGNFALVAAEVLQRRQGM-T 121
           G+ ES +   + +A G + D               K     G+  LVA      +  M  
Sbjct: 236 GIGESTIIKAISEAFGRTEDH------------VKKQNTELGDLGLVAKGSRSTQTMMFK 295

Query: 122 PGGLTIRELNDLLDRLA--LSENSRAEKTAVLSTLIQKTNAQEMKWIVMIILKDLKLGVS 181
           P  LT+ ++ D   ++A    ++S  +K   +  L+  T   E  ++  ++   L+LG S
Sbjct: 296 PEPLTVVKVFDTFRQIAKESGKDSNEKKKNRMKALLVATTDCEPLYLTRLLQAKLRLGFS 355

Query: 182 ---------EKSIFHEFHPD---------------AEDLFNVTCDLKLVCEKLRDRR--K 241
                    + ++++E H                  + +F V     ++   L       
Sbjct: 356 GQTVLAALGQAAVYNEEHSKPPPNTKSPLEEAAKIVKQVFTVLPVYDIIVPALLSGGVWN 415

Query: 242 RHKRQDIEVGKAVRPQLASRVSNANAAWKKLHGKEVVVECKFDGDRIQIH--KNGT-EIH 301
             K  +  +G  + P LA           K        E K+DG+R QIH  ++GT EI+
Sbjct: 416 LPKTCNFTLGVPIGPMLAKPTKGVAEILNKFQDIVFTCEYKYDGERAQIHFMEDGTFEIY 475

Query: 302 F--FSRNFLDHSEYGRGMSDVIVENILATRCILDGEMLVWDKSLNRFAEF-------GSN 361
                RN   + +    +S +   ++ +   ILD E++ +D+   +   F         N
Sbjct: 476 SRNAERNTGKYPDVALALSRLKKPSVKS--FILDCEVVAFDREKKKILPFQILSTRARKN 535

Query: 362 QEIGE----WTDVAFDVLYVGDTSVIHRSLKERHELLRDVVKPVKGRLEILVPNGGLNSD 421
             + +        AFD+LY+    +I  +LK R E L +  +   G  +           
Sbjct: 536 VNVNDIKVGVCIFAFDMLYLNGQQLIQENLKIRREKLYESFEEDPGYFQF---------- 595

Query: 422 CAGDPSWSQRAYNADDVERFFKNTIENRDEGIVIKDLGS--KWEPGDRSGKWLKLKPDYV 481
                + +  + + D++++F   +++   EG++IK L S   +EP  RS  WLKLK DY+
Sbjct: 596 -----ATAVTSNDIDEIQKFLDASVDVGCEGLIIKTLDSDATYEPAKRSNNWLKLKKDYM 655

Query: 482 RA-GSDLDALIIGGYYGSGRRGGEVAQFLMGLAERSPSNAYPRRFISFCRVGTGLTDEEL 541
            + G  +D + I  ++G G+R G    FL+   +          F S C++GTG +D  L
Sbjct: 656 DSIGDSVDLVPIAAFHGRGKRTGVYGAFLLACYD-----VDKEEFQSICKIGTGFSDAML 715

Query: 542 DAVTNKLRPYFRKSEYPKKTAPSFYQVTNNSKERPDVWIESPE----KSIILSITSDIRT 601
           D  ++ LR           T   +Y+V ++    PDVW E  E    K+  L+I+   R 
Sbjct: 716 DERSSSLR------SQVIATPKQYYRVGDSL--NPDVWFEPTEVWEVKAADLTISPVHRA 775

BLAST of MS007107 vs. TAIR 10
Match: AT1G66730.1 (DNA LIGASE 6 )

HSP 1 Score: 106.3 bits (264), Expect = 1.8e-22
Identity = 134/602 (22.26%), Postives = 248/602 (41.20%), Query Frame = 0

Query: 55   DRERGTYGLKESVLATCLIDALGISRDSDDALRLTNWRKGGAKTGANAGNFALVAAEVLQ 114
            D E     +  S++++ L +A GISR +          +    +  + G+ A +  +   
Sbjct: 819  DHENIELNIGGSLISSALEEACGISRST---------VRDMYNSLGDLGDVAQLCRQT-- 878

Query: 115  RRQGMTPGGLTIRELNDLLDRLAL---SENSRAEKTAVLSTLIQKTNAQEMKWIVMIILK 174
            ++  + P  L +R++   L ++++   + ++R +K  ++  L++    +E+K++V  + +
Sbjct: 879  QKLLVPPPPLLVRDVFSTLRKISVQTGTGSTRLKKNLIVK-LMRSCREKEIKFLVRTLAR 938

Query: 175  DLKLGVSEKS---------IFHEFHPD--------------------AEDLFNVTCDLKL 234
            +L++G   ++         + + F  D                      + +N+   L +
Sbjct: 939  NLRIGAMLRTVLPALGRAIVMNSFWNDHNKELSESCFREKLEGVSAAVVEAYNILPSLDV 998

Query: 235  VCEKLRDRRKRHKRQDIEV--GKAVRPQLASRVSNANAAWKKLHGKEVVVECKFDGDRIQ 294
            V   L D+        + +  G  ++P LA         +     K    E K+DG R Q
Sbjct: 999  VVPSLMDKDIEFSTSTLSMVPGIPIKPMLAKIAKGVQEFFNLSQEKAFTCEYKYDGQRAQ 1058

Query: 295  IHK--NGTEIHFFSRNFLDHSEYGRGMSDVIVENI--LATRCILDGEMLVWDK----SLN 354
            IHK  +GT +  FSRN  + +     + DVI +     A   +LD E++  D+     L 
Sbjct: 1059 IHKLLDGT-VCIFSRNGDETTSRFPDLVDVIKQFSCPAAETFMLDAEVVATDRINGNKLM 1118

Query: 355  RFAEFGSNQEIG-------------EWTDVAFDVLYVGDTSVIHRSLKERHELLRDVVKP 414
             F E  S +E G             E     FD+++V    ++   L+ER   L++V   
Sbjct: 1119 SFQEL-STRERGSKDALITTESIKVEVCVFVFDIMFVNGEQLLALPLRERRRRLKEVFPE 1178

Query: 415  VK-GRLEIL--VPNGGLNSDCAGDPSWSQRAYNADDVERFFKNTIENRDEGIVIK--DLG 474
             + G LE    +  G   +      + S+       +  F +   ++  EGI++K  D+ 
Sbjct: 1179 TRPGYLEYAKEITVGAEEASLNNHDTLSR-------INAFLEEAFQSSCEGIMVKSLDVN 1238

Query: 475  SKWEPGDRSGKWLKLKPDYVRA-GSDLDALIIGGYYGSGRRGGEVAQFLMGLAERSPSNA 534
            + + P  RS  WLK+K DYV   G  LD + IG +YG+GR+ G  + FLM        N 
Sbjct: 1239 AGYCPTKRSDSWLKVKRDYVDGLGDTLDLVPIGAWYGNGRKAGWYSPFLMAC-----FNP 1298

Query: 535  YPRRFISFCRVGTGLTDEELDAVTNKLRPYFRKSEYPKKTAPSFYQVTNNSKERPDVWIE 591
                F S CRV +G +    DA   +++ ++ + +   K  P +      + E PD+W  
Sbjct: 1299 ETEEFQSVCRVMSGFS----DAFYIEMKEFYSEDKILAKKPPYY-----RTGETPDMWFS 1358