MS006875 (gene) Bitter gourd (TR) v1

BLAST of MS006875 vs. NCBI nr
Match: XP_004147280.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >KGN64786.1 hypothetical protein Csa_014287 [Cucumis sativus])

HSP 1 Score: 879.4 bits (2271), Expect = 1.6e-251
Identity = 452/557 (81.15%), Postives = 497/557 (89.23%), Query Frame = 0

Query: 2   VRFTLQVLKGRWFTVFATLLIMAGAGATYLFGTYSKQIKSTLGYDQTTLNLMGFFKDFGA 61
           ++F LQV+ GRWFTVFAT LIMAGAGATYLFG YSKQIKS LGYDQTTLNLMGFFKD GA
Sbjct: 18  LQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNLMGFFKDLGA 77

Query: 62  NVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPAVWQMCLYICVGANSQ 121
           NVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAV+GRIAKP VWQMCLYICVGANSQ
Sbjct: 78  NVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAKPKVWQMCLYICVGANSQ 137

Query: 122 NFANTGALVTCIQNFPEGRGAMMGLLKGLTGLSGAILTQIYHAAYGDDATSLILLIAWLP 181
           NFANTGALVTCIQNFPE RGAMMGLLKG TGLSGAILT+IY A Y DDAT+LILLI WLP
Sbjct: 138 NFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDATALILLIGWLP 197

Query: 182 AALSVVFVFTIRKLSDDYQRQPNERKVFYHFLFVSIGLAVFIMAMNLAQKAVEFNRAAYA 241
           AA+SVVFVFTIR+L    +RQPNE++VFYHFL++SIGLAVFIM MN+ QK V+FN  AYA
Sbjct: 198 AAISVVFVFTIRRLRS--ERQPNEKRVFYHFLYISIGLAVFIMIMNIVQKKVQFNHTAYA 257

Query: 242 GSATVACLFLFLPLLIVIREELRICNTNKNQ--PIENPKPIEL-KPKIAVQESEEEKEY- 301
            SATV C+FLFLPLL+VIREELRI NT K+   PIE+P+P  + +PKI  +ES++  E  
Sbjct: 258 SSATVICVFLFLPLLVVIREELRIWNTKKSTSVPIESPQPKPIDEPKIITEESKQITEIQ 317

Query: 302 ------PPPESCFSNISHKPERGQDYTILQALLSWDMLVLFIATFCGLGTSLTSVDNLGQ 361
                 PPPESCFSNI  KP RG DYTILQALLS DM VLF+ATFCGLGTSLT+VDNLGQ
Sbjct: 318 KQNLATPPPESCFSNICQKPPRGDDYTILQALLSIDMFVLFVATFCGLGTSLTAVDNLGQ 377

Query: 362 IGESLGYPTKTVSSFVSLVSIWNYFGRIFSGFVSEILLEKFKFPRPLMMTLALLLSCVGQ 421
           IGESLGYP KTVSSFVSLVSIWNYFGRIF+GFVSE LL +FKFPRPLMMTL LLLSCVGQ
Sbjct: 378 IGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARFKFPRPLMMTLVLLLSCVGQ 437

Query: 422 LLIAFPVPGSVYLASIIIGFTFGAQLPLLFAIISELFGLKYFSTLFNFGQIASPLGSYIL 481
           LLIAFPVPGSVY+AS+IIGF+FGAQLPLLFAIISELFGLKYFSTLFN GQIASPLGSYIL
Sbjct: 438 LLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIASPLGSYIL 497

Query: 482 NVQVTGKLYDKQALKQLREKGLDRSAVKELVCLGKQCYRESFSLTAGVTFAGALISLILV 541
           NV+V G LYD +ALKQL+EKGLDRSAVKEL+C+GKQCYR+SFS+TA VTF GA++SL+LV
Sbjct: 498 NVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSITAIVTFVGAMVSLVLV 557

Query: 542 VRTREFYRGDIYKKFRD 549
           +RTREFY+GDIYKKFR+
Sbjct: 558 MRTREFYKGDIYKKFRE 572

BLAST of MS006875 vs. NCBI nr
Match: XP_038895725.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida])

HSP 1 Score: 874.4 bits (2258), Expect = 5.2e-250
Identity = 457/560 (81.61%), Postives = 493/560 (88.04%), Query Frame = 0

Query: 2   VRFTLQVLKGRWFTVFATLLIMAGAGATYLFGTYSKQIKSTLGYDQTTLNLMGFFKDFGA 61
           +RFTLQV+KGRWFTVFAT LIMAGAGATYLFG YSKQIK+TLGYDQTTLNLMGF KD GA
Sbjct: 16  LRFTLQVIKGRWFTVFATFLIMAGAGATYLFGVYSKQIKATLGYDQTTLNLMGFSKDLGA 75

Query: 62  NVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPAVWQMCLYICVGANSQ 121
           NVGVLSGLVAEVTPTWFVLLLGSALNF GYFMIWLAVTGRIAKP VWQMCLYICVGANSQ
Sbjct: 76  NVGVLSGLVAEVTPTWFVLLLGSALNFAGYFMIWLAVTGRIAKPQVWQMCLYICVGANSQ 135

Query: 122 NFANTGALVTCIQNFPEGRGAMMGLLKGLTGLSGAILTQIYHAAYGDDATSLILLIAWLP 181
           NFANTGALVTCIQNFPE RGAMMGLLKG TGLSGA+LTQIY A YGDDAT+LILLI WLP
Sbjct: 136 NFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAVLTQIYLAVYGDDATALILLIGWLP 195

Query: 182 AALSVVFVFTIRKLSDDYQRQPNERKVFYHFLFVSIGLAVFIMAMNLAQKAVEFNRAAYA 241
           AALSVVFVFTIR+L    +RQPNE++VFYHFL+VSIGLA+FIM MN+ QK VEFN  AYA
Sbjct: 196 AALSVVFVFTIRRLR--AERQPNEKRVFYHFLYVSIGLALFIMIMNIVQKKVEFNHTAYA 255

Query: 242 GSATVACLFLFLPLLIVIREELRICNTNK--NQPIEN----------PKPIELKPKIAVQ 301
            SATV C+FLFLPL IVIREEL + N  K    PI N          PKP++ + KI  +
Sbjct: 256 ISATVICVFLFLPLFIVIREELHLWNAKKTPTPPIPNENPQSQSQSQPKPVD-ESKIITE 315

Query: 302 ESEEEKEY--PPPESCFSNISHKPERGQDYTILQALLSWDMLVLFIATFCGLGTSLTSVD 361
           ES + KE   PPP+SCFSNI HKP RG DYTILQALLS DMLVLFIATFCGLGTSLT+VD
Sbjct: 316 ESNQIKEIPNPPPDSCFSNICHKPARGDDYTILQALLSVDMLVLFIATFCGLGTSLTAVD 375

Query: 362 NLGQIGESLGYPTKTVSSFVSLVSIWNYFGRIFSGFVSEILLEKFKFPRPLMMTLALLLS 421
           NLGQIGESLGYP KTVSSFVSLVSIWNYFGR+FSGFVSE LL KFKFPRPLMMTL LLLS
Sbjct: 376 NLGQIGESLGYPLKTVSSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRPLMMTLVLLLS 435

Query: 422 CVGQLLIAFPVPGSVYLASIIIGFTFGAQLPLLFAIISELFGLKYFSTLFNFGQIASPLG 481
           C+GQLLIAFPVPGSVYLAS+IIGF+FGAQLPLLFAIISELFGLKYFSTLFN GQIASPLG
Sbjct: 436 CIGQLLIAFPVPGSVYLASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIASPLG 495

Query: 482 SYILNVQVTGKLYDKQALKQLREKGLDRSAVKELVCLGKQCYRESFSLTAGVTFAGALIS 541
           SYILNV+V G LYD +ALKQL+EKGLDRSAVKEL C+GKQC+R+SF+L A VTF GA++S
Sbjct: 496 SYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELTCIGKQCFRKSFTLMAIVTFVGAMVS 555

Query: 542 LILVVRTREFYRGDIYKKFR 548
           L+LV+RTREFYRGDIYKKFR
Sbjct: 556 LVLVMRTREFYRGDIYKKFR 572

BLAST of MS006875 vs. NCBI nr
Match: XP_023519125.1 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 869.4 bits (2245), Expect = 1.7e-248
Identity = 449/557 (80.61%), Postives = 490/557 (87.97%), Query Frame = 0

Query: 1   VVRFTLQVLKGRWFTVFATLLIMAGAGATYLFGTYSKQIKSTLGYDQTTLNLMGFFKDFG 60
           +++F L VLKGRWFTVFAT LIMAGAGATYLFG YSKQIKSTLGYDQTTLNLMGFFKD G
Sbjct: 19  LLQFALHVLKGRWFTVFATFLIMAGAGATYLFGVYSKQIKSTLGYDQTTLNLMGFFKDLG 78

Query: 61  ANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPAVWQMCLYICVGANS 120
           ANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKP VWQMCLYICVGANS
Sbjct: 79  ANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPKVWQMCLYICVGANS 138

Query: 121 QNFANTGALVTCIQNFPEGRGAMMGLLKGLTGLSGAILTQIYHAAYGDDATSLILLIAWL 180
           QNFANTGALVTCIQNFPE RGAMMGLLKG TGLSGAILT+IY A YG+DAT+LILLIAWL
Sbjct: 139 QNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYLAVYGEDATALILLIAWL 198

Query: 181 PAALSVVFVFTIRKLSDDYQRQPNERKVFYHFLFVSIGLAVFIMAMNLAQKAVEFNRAAY 240
           PAALSVVF+F IRKL    +RQPNE +VFYHFL++SIGLA+FIM MN+ QK VEFN  AY
Sbjct: 199 PAALSVVFIFNIRKLK--AERQPNETRVFYHFLYISIGLAIFIMLMNIIQKKVEFNHTAY 258

Query: 241 AGSATVACLFLFLPLLIVIREELRICNTNKNQPIENPKPIELK---PKIAVQESEE---- 300
           A SATV C  LFLPL IVIREEL I N+  N PI    PIE K   PKI+++ES+E    
Sbjct: 259 ALSATVICALLFLPLFIVIREELHIWNSKINPPIPIENPIEPKIXEPKISIEESKEIKNN 318

Query: 301 --EKEYPPPESCFSNISHKPERGQDYTILQALLSWDMLVLFIATFCGLGTSLTSVDNLGQ 360
              K YPPP SCFS+I  KP RG DYTILQALLS DM+VLFIATFCGLGTSLT+VDNLGQ
Sbjct: 319 PDPKPYPPPPSCFSSICSKPARGDDYTILQALLSIDMMVLFIATFCGLGTSLTAVDNLGQ 378

Query: 361 IGESLGYPTKTVSSFVSLVSIWNYFGRIFSGFVSEILLEKFKFPRPLMMTLALLLSCVGQ 420
           IGESLGYP KTV SFVSLVSIWNYFGR+FSGFVSE LL KFKFPRPLMMTL LLLSC+GQ
Sbjct: 379 IGESLGYPLKTVKSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRPLMMTLVLLLSCIGQ 438

Query: 421 LLIAFPVPGSVYLASIIIGFTFGAQLPLLFAIISELFGLKYFSTLFNFGQIASPLGSYIL 480
           LLIAFP+PGSVY+ASIIIGF+FGAQLPLLFAIISELFGLKYFSTLFN GQIASPLGSYIL
Sbjct: 439 LLIAFPIPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIASPLGSYIL 498

Query: 481 NVQVTGKLYDKQALKQLREKGLDRSAVKELVCLGKQCYRESFSLTAGVTFAGALISLILV 540
           NV+V G +YD +ALKQL+E G+DR+AVKEL C+GK+CYR+SF+L A VTF GAL+SL+LV
Sbjct: 499 NVKVAGMMYDAEALKQLKEMGMDRAAVKELTCIGKRCYRKSFTLMAIVTFVGALVSLVLV 558

Query: 541 VRTREFYRGDIYKKFRD 549
           +RTREFYRGDIYK+FR+
Sbjct: 559 MRTREFYRGDIYKRFRE 573

BLAST of MS006875 vs. NCBI nr
Match: XP_023519124.1 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 868.6 bits (2243), Expect = 2.8e-248
Identity = 448/554 (80.87%), Postives = 489/554 (88.27%), Query Frame = 0

Query: 1   VVRFTLQVLKGRWFTVFATLLIMAGAGATYLFGTYSKQIKSTLGYDQTTLNLMGFFKDFG 60
           +++F L VLKGRWFTVFAT LIMAGAGATYLFG YSKQIKSTLGYDQTTLNLMGFFKD G
Sbjct: 19  LLQFALHVLKGRWFTVFATFLIMAGAGATYLFGVYSKQIKSTLGYDQTTLNLMGFFKDLG 78

Query: 61  ANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPAVWQMCLYICVGANS 120
           ANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKP VWQMCLYICVGANS
Sbjct: 79  ANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPKVWQMCLYICVGANS 138

Query: 121 QNFANTGALVTCIQNFPEGRGAMMGLLKGLTGLSGAILTQIYHAAYGDDATSLILLIAWL 180
           QNFANTGALVTCIQNFPE RGAMMGLLKG TGLSGAILT+IY A YG+DAT+LILLIAWL
Sbjct: 139 QNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYLAVYGEDATALILLIAWL 198

Query: 181 PAALSVVFVFTIRKLSDDYQRQPNERKVFYHFLFVSIGLAVFIMAMNLAQKAVEFNRAAY 240
           PAALSVVF+F IRKL    +RQPNE +VFYHFL++SIGLA+FIM MN+ QK VEFN  AY
Sbjct: 199 PAALSVVFIFNIRKLK--AERQPNETRVFYHFLYISIGLAIFIMLMNIIQKKVEFNHTAY 258

Query: 241 AGSATVACLFLFLPLLIVIREELRICNTNKNQPIENPKPIELKPKIAVQESEE------E 300
           A SATV C  LFLPL IVIREEL I N+  N PI    PIE  PKI+++ES+E       
Sbjct: 259 ALSATVICALLFLPLFIVIREELHIWNSKINPPIPIENPIE--PKISIEESKEIKNNPDP 318

Query: 301 KEYPPPESCFSNISHKPERGQDYTILQALLSWDMLVLFIATFCGLGTSLTSVDNLGQIGE 360
           K YPPP SCFS+I  KP RG DYTILQALLS DM+VLFIATFCGLGTSLT+VDNLGQIGE
Sbjct: 319 KPYPPPPSCFSSICSKPARGDDYTILQALLSIDMMVLFIATFCGLGTSLTAVDNLGQIGE 378

Query: 361 SLGYPTKTVSSFVSLVSIWNYFGRIFSGFVSEILLEKFKFPRPLMMTLALLLSCVGQLLI 420
           SLGYP KTV SFVSLVSIWNYFGR+FSGFVSE LL KFKFPRPLMMTL LLLSC+GQLLI
Sbjct: 379 SLGYPLKTVKSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRPLMMTLVLLLSCIGQLLI 438

Query: 421 AFPVPGSVYLASIIIGFTFGAQLPLLFAIISELFGLKYFSTLFNFGQIASPLGSYILNVQ 480
           AFP+PGSVY+ASIIIGF+FGAQLPLLFAIISELFGLKYFSTLFN GQIASPLGSYILNV+
Sbjct: 439 AFPIPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIASPLGSYILNVK 498

Query: 481 VTGKLYDKQALKQLREKGLDRSAVKELVCLGKQCYRESFSLTAGVTFAGALISLILVVRT 540
           V G +YD +ALKQL+E G+DR+AVKEL C+GK+CYR+SF+L A VTF GAL+SL+LV+RT
Sbjct: 499 VAGMMYDAEALKQLKEMGMDRAAVKELTCIGKRCYRKSFTLMAIVTFVGALVSLVLVMRT 558

Query: 541 REFYRGDIYKKFRD 549
           REFYRGDIYK+FR+
Sbjct: 559 REFYRGDIYKRFRE 568

BLAST of MS006875 vs. NCBI nr
Match: KAG6583553.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 867.8 bits (2241), Expect = 4.8e-248
Identity = 447/554 (80.69%), Postives = 488/554 (88.09%), Query Frame = 0

Query: 1   VVRFTLQVLKGRWFTVFATLLIMAGAGATYLFGTYSKQIKSTLGYDQTTLNLMGFFKDFG 60
           +++F L VLKGRWFTVFAT LIMAGAGATYLFG YSKQIKSTLGYDQTTLNLMGFFKD G
Sbjct: 19  LLQFALHVLKGRWFTVFATFLIMAGAGATYLFGVYSKQIKSTLGYDQTTLNLMGFFKDLG 78

Query: 61  ANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPAVWQMCLYICVGANS 120
           ANVGVLSGLVAEVTPTWFVLLLGS LNFTGYFMIWLAVTGRI KP VWQMCLYICVGANS
Sbjct: 79  ANVGVLSGLVAEVTPTWFVLLLGSVLNFTGYFMIWLAVTGRITKPKVWQMCLYICVGANS 138

Query: 121 QNFANTGALVTCIQNFPEGRGAMMGLLKGLTGLSGAILTQIYHAAYGDDATSLILLIAWL 180
           QNFANTGALVTCIQNFPE RGAMMGLLKG TGLSGAILT+IY A YG+DAT+LILLIAWL
Sbjct: 139 QNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYLAVYGEDATALILLIAWL 198

Query: 181 PAALSVVFVFTIRKLSDDYQRQPNERKVFYHFLFVSIGLAVFIMAMNLAQKAVEFNRAAY 240
           PAALSVVF+F IRKL    +RQPNE +VFYHFL++SIGLA+FIM MN+ QK VEFN  AY
Sbjct: 199 PAALSVVFIFNIRKLK--AERQPNETRVFYHFLYISIGLAIFIMLMNIIQKKVEFNHTAY 258

Query: 241 AGSATVACLFLFLPLLIVIREELRICNTNKNQPIENPKPIELKPKIAVQESEE------E 300
           A SATV C  LFLPL IVIREEL I N+  N PI    PIE  PKI+++ES+E       
Sbjct: 259 ALSATVICALLFLPLFIVIREELHIWNSKINPPIPIENPIE--PKISIEESKEIKNNPDP 318

Query: 301 KEYPPPESCFSNISHKPERGQDYTILQALLSWDMLVLFIATFCGLGTSLTSVDNLGQIGE 360
           K YPPP SCFS+I +KP RG DYTILQALLS DM+VLFIATFCGLGTSLT+VDNLGQIGE
Sbjct: 319 KPYPPPPSCFSSICNKPARGDDYTILQALLSIDMMVLFIATFCGLGTSLTAVDNLGQIGE 378

Query: 361 SLGYPTKTVSSFVSLVSIWNYFGRIFSGFVSEILLEKFKFPRPLMMTLALLLSCVGQLLI 420
           SLGYP KTV SFVSLVSIWNYFGR+FSGFVSE LL KFKFPRPLMMTL LLLSC+GQLLI
Sbjct: 379 SLGYPLKTVKSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRPLMMTLVLLLSCIGQLLI 438

Query: 421 AFPVPGSVYLASIIIGFTFGAQLPLLFAIISELFGLKYFSTLFNFGQIASPLGSYILNVQ 480
           AFP+PGSVY+ASIIIGF+FGAQLPLLFAIISELFGLKYFSTLFN GQIASPLGSYILNV+
Sbjct: 439 AFPIPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIASPLGSYILNVK 498

Query: 481 VTGKLYDKQALKQLREKGLDRSAVKELVCLGKQCYRESFSLTAGVTFAGALISLILVVRT 540
           V G +YD +ALKQL+E G+DR+AVKEL C+GKQCYR+SF+L A VTF GAL+SL+LV+RT
Sbjct: 499 VAGMMYDAEALKQLKEMGMDRAAVKELTCIGKQCYRKSFTLMAIVTFVGALVSLVLVMRT 558

Query: 541 REFYRGDIYKKFRD 549
           REFYRGDIYK+FR+
Sbjct: 559 REFYRGDIYKRFRE 568

BLAST of MS006875 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 9.1e-32
Identity = 133/538 (24.72%), Postives = 237/538 (44.05%), Query Frame = 0

Query: 12  RWFTVFATLLIMAGAGATYLFGTYSKQIKSTLGYDQTTLNLMGFFKDFGANVGVLSGLVA 71
           +W  + A + I A  G  + F  YS  +KS LG  Q  LN +    D G   G  SG+  
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 72  EVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPAVWQMCLYICVGAN-SQNFANTGALV 131
              P   VL   +A+ F GY + WL +T  I  P  + +    C+ A  S  + NT   +
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP--YSLVFLCCLLAGLSICWFNTACFI 162

Query: 132 TCIQNFPEGRGAMMGLLKGLTGLSGAILTQIYHAAYGDDATSLILLIAWLPAALSVVFVF 191
            CI++FP  R   + L     G+S A+ +  ++A     +   +LL + +P  +S   ++
Sbjct: 163 LCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVVSFAALY 222

Query: 192 -TIRKLSDDYQRQPNERKVFYH-FLFVSIGLAVFIMAMNLAQKAVEFNRAAYAGSATVAC 251
             + K S D     + R+   H F  +++   +    + L+  +    R  + G+  +  
Sbjct: 223 PVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIGAVVLLV 282

Query: 252 LFLFLPLLIVIREE-LRICNTNKNQPIENPKPIELKPKIAVQESEEEKEYPPPESCFSNI 311
             L  PLL+  R+  L + N   N   E+   + L     + E + +K     ++ + ++
Sbjct: 283 FPLCAPLLVYARDYFLPVINARLNH--ESSGYVMLN----IDELKNQKTSVSSKTGYEHM 342

Query: 312 SHKPE-----RGQDYTILQALLSWDMLVLFIATFCGLGTSLTSVDNLGQIGESLGYPTKT 371
               E      G +++    +   +  + +IA FCG    L   +NLGQI +SLG  + T
Sbjct: 343 GTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNSTT 402

Query: 372 VSSFVSLVSIWNYFGRIFSGFVSEILLEKFKFPRPLMMTLALLLSCVGQLLIAFPVPGSV 431
           +   V++ S +++FGR+ S    + + ++F+  R     +ALL + +   L+A       
Sbjct: 403 L---VTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQT 462

Query: 432 YL--ASIIIGFTFGAQLPLLFAIISELFGLKYFSTLFNFGQIASPLGSYILNVQVTGKLY 491
            L  A+ +IG + G       +I S+LFG        N      P+GS +L   +   +Y
Sbjct: 463 ALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGS-LLYGYIAASIY 522

Query: 492 DKQALKQLREKGLDRSAVKELVCLGKQCYRESFSLTAGVTFAGALISLILVVRTREFY 539
           +  A   +     D      +VC+G+ CY ++F     ++  G + SL L +RT+  Y
Sbjct: 523 EANASPDITPIVSD-----SIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of MS006875 vs. ExPASy TrEMBL
Match: A0A0A0LXR5 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G097655 PE=4 SV=1)

HSP 1 Score: 879.4 bits (2271), Expect = 7.8e-252
Identity = 452/557 (81.15%), Postives = 497/557 (89.23%), Query Frame = 0

Query: 2   VRFTLQVLKGRWFTVFATLLIMAGAGATYLFGTYSKQIKSTLGYDQTTLNLMGFFKDFGA 61
           ++F LQV+ GRWFTVFAT LIMAGAGATYLFG YSKQIKS LGYDQTTLNLMGFFKD GA
Sbjct: 18  LQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNLMGFFKDLGA 77

Query: 62  NVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPAVWQMCLYICVGANSQ 121
           NVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAV+GRIAKP VWQMCLYICVGANSQ
Sbjct: 78  NVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAKPKVWQMCLYICVGANSQ 137

Query: 122 NFANTGALVTCIQNFPEGRGAMMGLLKGLTGLSGAILTQIYHAAYGDDATSLILLIAWLP 181
           NFANTGALVTCIQNFPE RGAMMGLLKG TGLSGAILT+IY A Y DDAT+LILLI WLP
Sbjct: 138 NFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDATALILLIGWLP 197

Query: 182 AALSVVFVFTIRKLSDDYQRQPNERKVFYHFLFVSIGLAVFIMAMNLAQKAVEFNRAAYA 241
           AA+SVVFVFTIR+L    +RQPNE++VFYHFL++SIGLAVFIM MN+ QK V+FN  AYA
Sbjct: 198 AAISVVFVFTIRRLRS--ERQPNEKRVFYHFLYISIGLAVFIMIMNIVQKKVQFNHTAYA 257

Query: 242 GSATVACLFLFLPLLIVIREELRICNTNKNQ--PIENPKPIEL-KPKIAVQESEEEKEY- 301
            SATV C+FLFLPLL+VIREELRI NT K+   PIE+P+P  + +PKI  +ES++  E  
Sbjct: 258 SSATVICVFLFLPLLVVIREELRIWNTKKSTSVPIESPQPKPIDEPKIITEESKQITEIQ 317

Query: 302 ------PPPESCFSNISHKPERGQDYTILQALLSWDMLVLFIATFCGLGTSLTSVDNLGQ 361
                 PPPESCFSNI  KP RG DYTILQALLS DM VLF+ATFCGLGTSLT+VDNLGQ
Sbjct: 318 KQNLATPPPESCFSNICQKPPRGDDYTILQALLSIDMFVLFVATFCGLGTSLTAVDNLGQ 377

Query: 362 IGESLGYPTKTVSSFVSLVSIWNYFGRIFSGFVSEILLEKFKFPRPLMMTLALLLSCVGQ 421
           IGESLGYP KTVSSFVSLVSIWNYFGRIF+GFVSE LL +FKFPRPLMMTL LLLSCVGQ
Sbjct: 378 IGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARFKFPRPLMMTLVLLLSCVGQ 437

Query: 422 LLIAFPVPGSVYLASIIIGFTFGAQLPLLFAIISELFGLKYFSTLFNFGQIASPLGSYIL 481
           LLIAFPVPGSVY+AS+IIGF+FGAQLPLLFAIISELFGLKYFSTLFN GQIASPLGSYIL
Sbjct: 438 LLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIASPLGSYIL 497

Query: 482 NVQVTGKLYDKQALKQLREKGLDRSAVKELVCLGKQCYRESFSLTAGVTFAGALISLILV 541
           NV+V G LYD +ALKQL+EKGLDRSAVKEL+C+GKQCYR+SFS+TA VTF GA++SL+LV
Sbjct: 498 NVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSITAIVTFVGAMVSLVLV 557

Query: 542 VRTREFYRGDIYKKFRD 549
           +RTREFY+GDIYKKFR+
Sbjct: 558 MRTREFYKGDIYKKFRE 572

BLAST of MS006875 vs. ExPASy TrEMBL
Match: A0A6J1HPV2 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464958 PE=4 SV=1)

HSP 1 Score: 867.8 bits (2241), Expect = 2.3e-248
Identity = 447/554 (80.69%), Postives = 488/554 (88.09%), Query Frame = 0

Query: 1   VVRFTLQVLKGRWFTVFATLLIMAGAGATYLFGTYSKQIKSTLGYDQTTLNLMGFFKDFG 60
           +++F L VLKGRWFTVFAT LIMAGAGATYLFG YSKQIKSTLGYDQTTLNLMGFFKD G
Sbjct: 19  LLQFALHVLKGRWFTVFATFLIMAGAGATYLFGVYSKQIKSTLGYDQTTLNLMGFFKDLG 78

Query: 61  ANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPAVWQMCLYICVGANS 120
           ANVGVLSGLVAEVTPTWFVLLLGS LNFTGYFMIWLAVTGRI KP VWQMCLYICVGANS
Sbjct: 79  ANVGVLSGLVAEVTPTWFVLLLGSVLNFTGYFMIWLAVTGRITKPKVWQMCLYICVGANS 138

Query: 121 QNFANTGALVTCIQNFPEGRGAMMGLLKGLTGLSGAILTQIYHAAYGDDATSLILLIAWL 180
           QNFANTGALVTCIQNFPE RGAMMGLLKG TGLSGAILT+IY A YG+DAT+LILLIAWL
Sbjct: 139 QNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYLAVYGEDATALILLIAWL 198

Query: 181 PAALSVVFVFTIRKLSDDYQRQPNERKVFYHFLFVSIGLAVFIMAMNLAQKAVEFNRAAY 240
           PAALSVVF+F IRKL    +RQPNE +VFYHFL++SIGLA+FIM MN+ QK VEFN  AY
Sbjct: 199 PAALSVVFIFNIRKLK--AERQPNETRVFYHFLYISIGLAIFIMLMNIIQKKVEFNHTAY 258

Query: 241 AGSATVACLFLFLPLLIVIREELRICNTNKNQPIENPKPIELKPKIAVQESEE------E 300
           A SATV C  LFLPL IVIREEL I N+  N PI    PIE  PKI+++ES+E       
Sbjct: 259 ALSATVICALLFLPLFIVIREELHIWNSKINPPIPIENPIE--PKISIEESKEIKNNPDP 318

Query: 301 KEYPPPESCFSNISHKPERGQDYTILQALLSWDMLVLFIATFCGLGTSLTSVDNLGQIGE 360
           K YPPP SCFS+I +KP RG DYTILQALLS DM+VLFIATFCGLGTSLT+VDNLGQIGE
Sbjct: 319 KPYPPPPSCFSSICNKPARGDDYTILQALLSIDMMVLFIATFCGLGTSLTAVDNLGQIGE 378

Query: 361 SLGYPTKTVSSFVSLVSIWNYFGRIFSGFVSEILLEKFKFPRPLMMTLALLLSCVGQLLI 420
           SLGYP KTV SFVSLVSIWNYFGR+FSGFVSE LL KFKFPRPLMMTL LLLSC+GQLLI
Sbjct: 379 SLGYPLKTVKSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRPLMMTLVLLLSCIGQLLI 438

Query: 421 AFPVPGSVYLASIIIGFTFGAQLPLLFAIISELFGLKYFSTLFNFGQIASPLGSYILNVQ 480
           AFP+PGSVY+ASIIIGF+FGAQLPLLFAIISELFGLKYFSTLFN GQIASPLGSYILNV+
Sbjct: 439 AFPIPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIASPLGSYILNVK 498

Query: 481 VTGKLYDKQALKQLREKGLDRSAVKELVCLGKQCYRESFSLTAGVTFAGALISLILVVRT 540
           V G +YD +ALKQL+E G+DR+AVKEL C+GKQCYR+SF+L A VTF GAL+SL+LV+RT
Sbjct: 499 VAGMMYDAEALKQLKEMGMDRAAVKELTCIGKQCYRKSFTLMAIVTFVGALVSLVLVMRT 558

Query: 541 REFYRGDIYKKFRD 549
           REFYRGDIYK+FR+
Sbjct: 559 REFYRGDIYKRFRE 568

BLAST of MS006875 vs. ExPASy TrEMBL
Match: A0A5A7UED5 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold609G00250 PE=4 SV=1)

HSP 1 Score: 867.5 bits (2240), Expect = 3.1e-248
Identity = 450/558 (80.65%), Postives = 493/558 (88.35%), Query Frame = 0

Query: 2   VRFTLQVLKGRWFTVFATLLIMAGAGATYLFGTYSKQIKSTLGYDQTTLNLMGFFKDFGA 61
           ++F LQV+ GRWFTVFAT LIMAGAGATYLFG YSKQIKS LGYDQTTLNLMGFFKD GA
Sbjct: 18  LQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNLMGFFKDLGA 77

Query: 62  NVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPAVWQMCLYICVGANSQ 121
           NVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKP VWQMCLYICVGANSQ
Sbjct: 78  NVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPKVWQMCLYICVGANSQ 137

Query: 122 NFANTGALVTCIQNFPEGRGAMMGLLKGLTGLSGAILTQIYHAAYGDDATSLILLIAWLP 181
           NFANTGALVTCIQNFPE RGAMMGLLKG TGLSGAILT+IY A Y DDAT+LILLI WLP
Sbjct: 138 NFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDATALILLIGWLP 197

Query: 182 AALSVVFVFTIRKLSDDYQRQPNERKVFYHFLFVSIGLAVFIMAMNLAQKAVEFNRAAYA 241
           AA+SVVFVFTIR+L    +RQPNE++VFYHFL++SIGLAVFIM MN+ QK VEFN  AY 
Sbjct: 198 AAISVVFVFTIRRLK--AERQPNEKRVFYHFLYISIGLAVFIMIMNIVQKKVEFNHTAYT 257

Query: 242 GSATVACLFLFLPLLIVIREELRICNTNKNQ--PIENPKPIEL-KPKIAVQES------- 301
            SATV C+FLFLPLLIVIREELR+ N NK+   PIENP+P  + +PKI  +ES       
Sbjct: 258 LSATVICIFLFLPLLIVIREELRLWNANKSPPIPIENPQPKPIDEPKIITEESNQITKIQ 317

Query: 302 EEEKEYPPPE-SCFSNISHKPERGQDYTILQALLSWDMLVLFIATFCGLGTSLTSVDNLG 361
            +    PPPE +CFSNI  KP RG DYTILQALLS DM VLFIATFCGLGTSLT+VDNLG
Sbjct: 318 NQSPPAPPPEVACFSNICRKPPRGDDYTILQALLSIDMFVLFIATFCGLGTSLTAVDNLG 377

Query: 362 QIGESLGYPTKTVSSFVSLVSIWNYFGRIFSGFVSEILLEKFKFPRPLMMTLALLLSCVG 421
           QIGESLGYP KTVSSFVSLVSIWNYFGRIF+GFVSE LL ++KFPRPLMMTL LLLSC+G
Sbjct: 378 QIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARYKFPRPLMMTLVLLLSCIG 437

Query: 422 QLLIAFPVPGSVYLASIIIGFTFGAQLPLLFAIISELFGLKYFSTLFNFGQIASPLGSYI 481
           QLLIAFPVPGSVY+AS+IIGF+FGAQLPLLFAIISELFGLKYFSTLFN GQIASPLGSYI
Sbjct: 438 QLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIASPLGSYI 497

Query: 482 LNVQVTGKLYDKQALKQLREKGLDRSAVKELVCLGKQCYRESFSLTAGVTFAGALISLIL 541
           LNV+V G LYD +ALKQL+EKGLDRSAVKEL C+GK+CYR+SF++T  VTFAGA++SLIL
Sbjct: 498 LNVKVAGMLYDIEALKQLKEKGLDRSAVKELTCIGKECYRKSFTITTIVTFAGAMVSLIL 557

Query: 542 VVRTREFYRGDIYKKFRD 549
           V+RTREFY+GDIYKKFR+
Sbjct: 558 VMRTREFYKGDIYKKFRE 573

BLAST of MS006875 vs. ExPASy TrEMBL
Match: A0A1S3CP80 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103503227 PE=4 SV=1)

HSP 1 Score: 867.5 bits (2240), Expect = 3.1e-248
Identity = 450/558 (80.65%), Postives = 493/558 (88.35%), Query Frame = 0

Query: 2   VRFTLQVLKGRWFTVFATLLIMAGAGATYLFGTYSKQIKSTLGYDQTTLNLMGFFKDFGA 61
           ++F LQV+ GRWFTVFAT LIMAGAGATYLFG YSKQIKS LGYDQTTLNLMGFFKD GA
Sbjct: 18  LQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNLMGFFKDLGA 77

Query: 62  NVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPAVWQMCLYICVGANSQ 121
           NVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKP VWQMCLYICVGANSQ
Sbjct: 78  NVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPKVWQMCLYICVGANSQ 137

Query: 122 NFANTGALVTCIQNFPEGRGAMMGLLKGLTGLSGAILTQIYHAAYGDDATSLILLIAWLP 181
           NFANTGALVTCIQNFPE RGAMMGLLKG TGLSGAILT+IY A Y DDAT+LILLI WLP
Sbjct: 138 NFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDATALILLIGWLP 197

Query: 182 AALSVVFVFTIRKLSDDYQRQPNERKVFYHFLFVSIGLAVFIMAMNLAQKAVEFNRAAYA 241
           AA+SVVFVFTIR+L    +RQPNE++VFYHFL++SIGLAVFIM MN+ QK VEFN  AY 
Sbjct: 198 AAISVVFVFTIRRLK--AERQPNEKRVFYHFLYISIGLAVFIMIMNIVQKKVEFNHTAYT 257

Query: 242 GSATVACLFLFLPLLIVIREELRICNTNKNQ--PIENPKPIEL-KPKIAVQES------- 301
            SATV C+FLFLPLLIVIREELR+ N NK+   PIENP+P  + +PKI  +ES       
Sbjct: 258 LSATVICIFLFLPLLIVIREELRLWNANKSPPIPIENPQPKPIDEPKIITEESNQITKIQ 317

Query: 302 EEEKEYPPPE-SCFSNISHKPERGQDYTILQALLSWDMLVLFIATFCGLGTSLTSVDNLG 361
            +    PPPE +CFSNI  KP RG DYTILQALLS DM VLFIATFCGLGTSLT+VDNLG
Sbjct: 318 NQSPPAPPPEVACFSNICRKPPRGDDYTILQALLSIDMFVLFIATFCGLGTSLTAVDNLG 377

Query: 362 QIGESLGYPTKTVSSFVSLVSIWNYFGRIFSGFVSEILLEKFKFPRPLMMTLALLLSCVG 421
           QIGESLGYP KTVSSFVSLVSIWNYFGRIF+GFVSE LL ++KFPRPLMMTL LLLSC+G
Sbjct: 378 QIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARYKFPRPLMMTLVLLLSCIG 437

Query: 422 QLLIAFPVPGSVYLASIIIGFTFGAQLPLLFAIISELFGLKYFSTLFNFGQIASPLGSYI 481
           QLLIAFPVPGSVY+AS+IIGF+FGAQLPLLFAIISELFGLKYFSTLFN GQIASPLGSYI
Sbjct: 438 QLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIASPLGSYI 497

Query: 482 LNVQVTGKLYDKQALKQLREKGLDRSAVKELVCLGKQCYRESFSLTAGVTFAGALISLIL 541
           LNV+V G LYD +ALKQL+EKGLDRSAVKEL C+GK+CYR+SF++T  VTFAGA++SLIL
Sbjct: 498 LNVKVAGMLYDIEALKQLKEKGLDRSAVKELTCIGKECYRKSFTITTIVTFAGAMVSLIL 557

Query: 542 VVRTREFYRGDIYKKFRD 549
           V+RTREFY+GDIYKKFR+
Sbjct: 558 VMRTREFYKGDIYKKFRE 573

BLAST of MS006875 vs. ExPASy TrEMBL
Match: A0A6J1I026 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469244 PE=4 SV=1)

HSP 1 Score: 863.2 bits (2229), Expect = 5.8e-247
Identity = 446/554 (80.51%), Postives = 486/554 (87.73%), Query Frame = 0

Query: 1   VVRFTLQVLKGRWFTVFATLLIMAGAGATYLFGTYSKQIKSTLGYDQTTLNLMGFFKDFG 60
           +++F L VLKGRWFTVFAT LIMAGAGATYLFG YSKQIKSTLGYDQTTLNLMGFFKD G
Sbjct: 19  LLQFALHVLKGRWFTVFATFLIMAGAGATYLFGVYSKQIKSTLGYDQTTLNLMGFFKDLG 78

Query: 61  ANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPAVWQMCLYICVGANS 120
           ANVGVLSGLVAEVTPTWFVLLLGS LNFTGYFMIWLAVTGRIAKP VWQMCLYICVGANS
Sbjct: 79  ANVGVLSGLVAEVTPTWFVLLLGSVLNFTGYFMIWLAVTGRIAKPKVWQMCLYICVGANS 138

Query: 121 QNFANTGALVTCIQNFPEGRGAMMGLLKGLTGLSGAILTQIYHAAYGDDATSLILLIAWL 180
           QNFANTGALVTCIQNFPE RGAMMGLLKG TGLSGAILTQIY A YG+DAT+LILLIAWL
Sbjct: 139 QNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTQIYLAVYGEDATALILLIAWL 198

Query: 181 PAALSVVFVFTIRKLSDDYQRQPNERKVFYHFLFVSIGLAVFIMAMNLAQKAVEFNRAAY 240
           PAALSVVF+F IRKL    +R+PNE +VFYHFL+VSIGLA+FIM MN+ QK VEFN  AY
Sbjct: 199 PAALSVVFIFNIRKLK--AERRPNETRVFYHFLYVSIGLAIFIMLMNIIQKEVEFNHTAY 258

Query: 241 AGSATVACLFLFLPLLIVIREELRICNTNKNQPIENPKPIELKPKIAVQESEE------E 300
           A SATV C  LFLPL IVIREEL+I N+  N PI    P E  PKI+  ES+E       
Sbjct: 259 ALSATVICALLFLPLFIVIREELQIWNSKINPPIPIENPTE--PKISTDESKEIKNNPDP 318

Query: 301 KEYPPPESCFSNISHKPERGQDYTILQALLSWDMLVLFIATFCGLGTSLTSVDNLGQIGE 360
           K YPPP SCFS+I  KP RG DYTILQALLS DM+VLFIATFCGLGTSLT+VDNLGQIGE
Sbjct: 319 KPYPPPPSCFSSICDKPARGDDYTILQALLSIDMMVLFIATFCGLGTSLTAVDNLGQIGE 378

Query: 361 SLGYPTKTVSSFVSLVSIWNYFGRIFSGFVSEILLEKFKFPRPLMMTLALLLSCVGQLLI 420
           SLGYP KTV SFVSLVSIWNYFGR+FSGFVSE LL KFKFPRPLMMTL LLLSC+GQLLI
Sbjct: 379 SLGYPLKTVKSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRPLMMTLVLLLSCIGQLLI 438

Query: 421 AFPVPGSVYLASIIIGFTFGAQLPLLFAIISELFGLKYFSTLFNFGQIASPLGSYILNVQ 480
           AFP+PGSVY+AS+IIGF+FGAQLPLLFAIISELFGLKYFSTLFN GQIASPLGSYILNV+
Sbjct: 439 AFPIPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCGQIASPLGSYILNVK 498

Query: 481 VTGKLYDKQALKQLREKGLDRSAVKELVCLGKQCYRESFSLTAGVTFAGALISLILVVRT 540
           V G +YD +ALKQL+E G+DR+AVKEL C+GK+CYR+SF+L A VTF GAL+SL+LV+RT
Sbjct: 499 VAGMMYDAEALKQLKEMGIDRAAVKELTCIGKRCYRKSFTLMAIVTFVGALVSLVLVMRT 558

Query: 541 REFYRGDIYKKFRD 549
           REFYRGDIYK+FR+
Sbjct: 559 REFYRGDIYKRFRE 568

BLAST of MS006875 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 762.7 bits (1968), Expect = 2.0e-220
Identity = 392/556 (70.50%), Postives = 456/556 (82.01%), Query Frame = 0

Query: 2   VRFTLQVLKGRWFTVFATLLIMAGAGATYLFGTYSKQIKSTLGYDQTTLNLMGFFKDFGA 61
           VRF +    GRWF VFA+ LIMA AGATYLFGTYSK IKSTLGYDQTTLNL+GFFKD GA
Sbjct: 7   VRFLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGA 66

Query: 62  NVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPAVWQMCLYICVGANSQ 121
           NVGVLSGL+AEVTPTWFVL +GSA+NF GYFMIWL VTG++AKP VWQMCLYIC+GANSQ
Sbjct: 67  NVGVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQ 126

Query: 122 NFANTGALVTCIQNFPEGRGAMMGLLKGLTGLSGAILTQIYHAAYGDDATSLILLIAWLP 181
           NFANTGALVTC++NFPE RG M+GLLKG  GLSGAI TQ+Y A YG D+ SLILLIAWLP
Sbjct: 127 NFANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAWLP 186

Query: 182 AALSVVFVFTIRKLSDDYQRQPNERKVFYHFLFVSIGLAVFIMAMNLAQKAVEFNRAAYA 241
           AA+S+VFV+ IR+      RQ NE  VFY FL++SI LA+F+MAMN+A+K V F++AAYA
Sbjct: 187 AAVSLVFVYLIRE--KKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYA 246

Query: 242 GSATVACLFLFLPLLIVIREELRICNTNKNQPIENPKPIEL-KPK--------IAVQESE 301
            SAT+ C  LF+PL + +++EL + N  K  PIE P  +++ KPK         A + + 
Sbjct: 247 ASATICCALLFVPLTVSVKQELEVWNMMK-LPIEEPSEVKVEKPKKELDLDQDKAAKVNG 306

Query: 302 EEKEYPPPESCFSNISHKPERGQDYTILQALLSWDMLVLFIATFCGLGTSLTSVDNLGQI 361
           EEKE    +SCFS +   P RG+DYTILQALLS DM++LF+ATFCGLG+SLT+VDNLGQI
Sbjct: 307 EEKE---TKSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQI 366

Query: 362 GESLGYPTKTVSSFVSLVSIWNYFGRIFSGFVSEILLEKFKFPRPLMMTLALLLSCVGQL 421
           GESLGYP  TVSSFVSLVSIWNYFGR+FSGFVSE LL K+K PRPLMMTL LLLSC G L
Sbjct: 367 GESLGYPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHL 426

Query: 422 LIAFPVPGSVYLASIIIGFTFGAQLPLLFAIISELFGLKYFSTLFNFGQIASPLGSYILN 481
           LIAFPVPGSVY+ASI++GF+FGAQLPLLFAIISELFGLKY+STLFN GQ+ASPLGSYILN
Sbjct: 427 LIAFPVPGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILN 486

Query: 482 VQVTGKLYDKQALKQLREKGLDRSAVKELVCLGKQCYRESFSLTAGVTFAGALISLILVV 541
           V+VTG LYDK+ALKQL  +GL R  VK+L CLG QCY+  F + A VTF GAL+SL L +
Sbjct: 487 VRVTGMLYDKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAI 546

Query: 542 RTREFYRGDIYKKFRD 549
           RTREFY+GDIYKKFR+
Sbjct: 547 RTREFYKGDIYKKFRE 556

BLAST of MS006875 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 652.5 bits (1682), Expect = 3.0e-187
Identity = 330/555 (59.46%), Postives = 423/555 (76.22%), Query Frame = 0

Query: 5   TLQVLKGRWFTVFATLLIMAGAGATYLFGTYSKQIKSTLGYDQTTLNLMGFFKDFGANVG 64
           T+Q+L GRWF  F +LLIM+ AGATY+FG YS  IK TLGYDQTTLNL+ FFKD GANVG
Sbjct: 14  TIQILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVG 73

Query: 65  VLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPAVWQMCLYICVGANSQNFA 124
           VL+GL+ EVTP WF+LL+G+ LNF GYFMIWLAVT RI+KP VW MCLYICVGANSQ+FA
Sbjct: 74  VLAGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFA 133

Query: 125 NTGALVTCIQNFPEGRGAMMGLLKGLTGLSGAILTQIYHAAYGDDATSLILLIAWLPAAL 184
           NTG+LVTC++NFPE RG ++G+LKG  GLSGAI+TQ+Y A YG+D   LIL+I WLPA +
Sbjct: 134 NTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIGWLPAIV 193

Query: 185 SVVFVFTIRKLSDDYQRQPNERKVFYHFLFVSIGLAVFIMAMNLAQKAVEFNRAAYAGSA 244
           S  F+ TIR +    +RQ NE KVFY+FL++S+GLA F+M + +  K   F ++ + GSA
Sbjct: 194 SFAFLRTIRIMK--VKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSA 253

Query: 245 TVACLFLFLPLLIVIREELRICNTNKNQPIENPKPIEL---KPKIAV--------QESEE 304
            V  + L LP+++VI EE ++    K   + +P PI +   KPK+          +ES+E
Sbjct: 254 AVVIVLLLLPIIVVILEEKKLWK-EKQVALNDPAPINVVTEKPKLDSSEFKDDDGEESKE 313

Query: 305 EKEYPPPESCFSNISHKPERGQDYTILQALLSWDMLVLFIATFCGLGTSLTSVDNLGQIG 364
             E     SC++ + + PERG DYTILQAL S DML+LF+AT CG+G +LT++DNLGQIG
Sbjct: 314 VVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIG 373

Query: 365 ESLGYPTKTVSSFVSLVSIWNYFGRIFSGFVSEILLEKFKFPRPLMMTLALLLSCVGQLL 424
            SLGYP ++VS+FVSLVSIWNY+GR+ SG VSEI L K+KFPRPLM+T+ LLLSC G LL
Sbjct: 374 NSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLL 433

Query: 425 IAFPVPGSVYLASIIIGFTFGAQLPLLFAIISELFGLKYFSTLFNFGQIASPLGSYILNV 484
           IAF VPG +Y+AS+IIGF FGAQ PLLFAIISE+FGLKY+STL+NFG +ASP+GSY+LNV
Sbjct: 434 IAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLNV 493

Query: 485 QVTGKLYDKQALKQLREKGLDRSAVKELVCLGKQCYRESFSLTAGVTFAGALISLILVVR 544
           +V G LYD +A KQ +  G  R   ++L C+G  C++ SF + A VT  G L+S++LV+R
Sbjct: 494 RVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVIR 553

Query: 545 TREFYRGDIYKKFRD 549
           T++FY+ DIYKKFR+
Sbjct: 554 TKKFYKSDIYKKFRE 565

BLAST of MS006875 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 325.1 bits (832), Expect = 1.1e-88
Identity = 197/571 (34.50%), Postives = 305/571 (53.42%), Query Frame = 0

Query: 9   LKGRWFTVFATLLIMAGAGATYLFGTYSKQIKSTLGYDQTTLNLMGFFKDFGANVGVLSG 68
           +  RW    A + I + AG  YLFG+ S  IKS+L Y+Q  L+ +G  KD G +VG ++G
Sbjct: 13  INNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIAG 72

Query: 69  LVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPAVWQMCLYICVGANSQNFANTGA 128
            ++E+ P W  LL+G+  N  GY  +WL VTGR     +W MC+ I VG N + + NTGA
Sbjct: 73  TLSEILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTGA 132

Query: 129 LVTCIQNFPEGRGAMMGLLKGLTGLSGAILTQIYHAAYGDDATSLILLIAWLPAALSVVF 188
           LV+ +QNFP+ RG ++G+LKG  GL GAI++QIY   +  +  SLIL++A  PA + V  
Sbjct: 133 LVSGVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVAVTPAVVVVCL 192

Query: 189 VFTIRKLSDDYQRQPNERKVFYHFLFVSIGLAVFIMAMNLAQKAVEFNRAAYAGSATVAC 248
           +F IR +    Q +P +   F     V + LA ++M++ L Q  V  +         V  
Sbjct: 193 MFFIRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITVFTIVLF 252

Query: 249 LFLFLPLLIVIREELRICNTNKNQPIENP--------KPIELKPKIAVQESEEEK----E 308
           + L +P+L+ I           +  IE P        +P    P + + E E+EK    +
Sbjct: 253 VILVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLILSEVEDEKPKDVD 312

Query: 309 YPPPESCFSNISH--------------------KPERGQDYTILQALLSWDMLVLFIATF 368
             P       I+H                     P RG+D+T+ QAL+  D  ++F +  
Sbjct: 313 LLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLIFFSLL 372

Query: 369 CGLGTSLTSVDNLGQIGESLGYPTKTVSSFVSLVSIWNYFGRIFSGFVSEILLEKFKFPR 428
            G G+ LT +DNLGQ+ +SLGY    V   VS++SIWN+ GRI  G+ SE+++  + +PR
Sbjct: 373 LGSGSGLTVIDNLGQMSQSLGYDNTHV--LVSMISIWNFLGRIGGGYFSELVVRDYAYPR 432

Query: 429 PLMMTLALLLSCVGQLLIAFPVPGSVYLASIIIGFTFGAQLPLLFAIISELFGLKYFSTL 488
           P+ M +A L+  VG +  A+  PG++Y+ +++IG  +GA   ++ A  SELFGLK F  L
Sbjct: 433 PVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFGLKKFGAL 492

Query: 489 FNFGQIASPLGSYILNVQVTGKLYDKQALKQLREKGLDRSAVKELVCLGKQCYRESFSLT 548
           +NF  +A+P GS + +  +   +YD++A +Q      D      L C G  C+  +  + 
Sbjct: 493 YNFLTLANPAGSLVFSGMIASSIYDREAERQAHGSVFDPD--DALRCNGSICFFLTSLIM 552

BLAST of MS006875 vs. TAIR 10
Match: AT3G01930.2 (Major facilitator superfamily protein )

HSP 1 Score: 319.7 bits (818), Expect = 4.6e-87
Identity = 195/577 (33.80%), Postives = 308/577 (53.38%), Query Frame = 0

Query: 9   LKGRWFTVFATLLIMAGAGATYLFGTYSKQIKSTLGYDQTTLNLMGFFKDFGANVGVLSG 68
           +  RW    A + I + AG  YLFG+ S  IKS+L Y+Q  L+ +G  KD G +VG L+G
Sbjct: 13  INNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKQLSRLGVAKDLGDSVGFLAG 72

Query: 69  LVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPAVWQMCLYICVGANSQNFANTGA 128
            ++E+ P W  LL+GS  N  GY  +WL VTGR     +W MC+ I VG N + + NT A
Sbjct: 73  TLSEILPLWAALLVGSVQNLVGYGWVWLIVTGRAPILPLWAMCILIFVGNNGETYFNTAA 132

Query: 129 LVTCIQNFPEGRGAMMGLLKGLTGLSGAILTQIYHAAYGDDATSLILLIAWLPAALSVVF 188
           LV+ +QNFP+ RG ++G+LKG  GL GAIL+Q+Y   +  D  SLI ++A  P+ + V  
Sbjct: 133 LVSGVQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHSSDRASLIFMVAVAPSVVVVPL 192

Query: 189 VFTIRKLSDDYQRQPNERKVFYHFLFVSIGLAVFIMAMNLAQKAVEFNRAAYAGSATVAC 248
           +F IR +    Q + ++   F     V I LA ++MA+ L +  ++ + +       V  
Sbjct: 193 MFFIRPVGGHRQIRSSDATSFTVIYAVCILLAAYLMAVMLVEDFIDLSHSIIIAFTVVLF 252

Query: 249 LFLFLPLLIVIREELRICNTNKNQPIENP-------------KPIELKPKIAVQESEEEK 308
             L +P+ I I       +T+    +E P                +  P++   E E+EK
Sbjct: 253 AILLVPIFIPIATSCFTASTDPCDTLEEPLLGDQQGQDPGQSTTPDHGPELIFSEVEDEK 312

Query: 309 ----EYPPPESCFSNIS--------------------HKPERGQDYTILQALLSWDMLVL 368
               +  P       I+                      P RG+D+T+ QAL+  D  ++
Sbjct: 313 PKEVDLLPAVERHKRIAQLQAKLMQAAAEGAVRVKRRRGPHRGEDFTLTQALVKADFWLI 372

Query: 369 FIATFCGLGTSLTSVDNLGQIGESLGYPTKTVSSFVSLVSIWNYFGRIFSGFVSEILLEK 428
           F +   G G+ LT +DNLGQ+ +SLGY    V  FVS++SIWN+ GRI  G+ SE+++  
Sbjct: 373 FFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHV--FVSMISIWNFLGRIGGGYFSELIVRD 432

Query: 429 FKFPRPLMMTLALLLSCVGQLLIAFPVPGSVYLASIIIGFTFGAQLPLLFAIISELFGLK 488
           + +PRP+ + +A L+  VG +  A+  PG++++ +++IG  +GA   ++ A  SELFGLK
Sbjct: 433 YAYPRPVAIAVAQLVMSVGHIFFAYGWPGAMHIGTLLIGLGYGAHWAIVPATASELFGLK 492

Query: 489 YFSTLFNFGQIASPLGSYILNVQVTGKLYDKQALKQLREKGLDRSAVKELVCLGKQCYRE 548
            F  L+NF  +A+P GS + +  +   +YD++A +Q +    +   V  L C G  CY  
Sbjct: 493 KFGALYNFLTLANPAGSLVFSGLIASSIYDREAERQAQGSLFNPDDV--LRCRGSICYFL 552

BLAST of MS006875 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 318.5 bits (815), Expect = 1.0e-86
Identity = 192/556 (34.53%), Postives = 306/556 (55.04%), Query Frame = 0

Query: 6   LQVLKGRWFTVFATLLIMAGAGATYLFGTYSKQIKSTLGYDQTTLNLMGFFKDFGANVGV 65
           +++L+ +W  + A++ I   +GA+Y FG YS  +KST  YDQ+TL+ +  FKD GAN GV
Sbjct: 1   MEILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGV 60

Query: 66  LSGLVAEVTPT----------------WFVLLLGSALNFTGYFMIWLAVTGRIAKPAVWQ 125
            SGL+     +                W VL +G+   F GYF+IW +VTG I KP V  
Sbjct: 61  FSGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPL 120

Query: 126 MCLYICVGANSQNFANTGALVTCIQNFPEGRGAMMGLLKGLTGLSGAILTQIYHAAYGDD 185
           MCL++ + A SQ F NT  +V+ ++NF +  G  +G++KG  GLSGAIL Q+Y      D
Sbjct: 121 MCLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGD 180

Query: 186 ATSLILLIAWLPAALSVVFVFTIRKLSDDYQRQPNERKVFYHFLFVSIGLAVFIMAMNLA 245
             S ILL+A  P  LS++ +  +R          +++K       VS+ +A ++M + + 
Sbjct: 181 PASFILLLAVTPTVLSLLVMPLVRIYETSV---ADDKKHLNGLSAVSLIIAAYLMIIIIL 240

Query: 246 QKAVEFNRAAYAGSATVACLFLFLPLLIVIREELRICNTNKNQPIENPKPIELKPKIAVQ 305
           +    F  +++A   T+ CL + L L ++I    +     K  P  +  P+   PK    
Sbjct: 241 KNT--FGLSSWANIVTLVCLLVMLALPLLIARRAQRDGMEKTVP-HDYSPLISSPKATTS 300

Query: 306 ESEEEKEYPPPESCFSNISHKPERGQDYTILQALLSWDMLVLFIATFCGLGTSLTSVDNL 365
            ++  +     E+  S         ++  +LQA+      +LF+A  CG+G+ L++++N+
Sbjct: 301 GNQSSEGDSKVEAGLS---------ENLNLLQAMKKLSFWLLFLAMICGMGSGLSTINNI 360

Query: 366 GQIGESLGYPTKTVSSFVSLVSIWNYFGRIFSGFVSEILLEKFKFPRPLMMTLALLLSCV 425
            QIGESL Y +  ++S VSL SIWN+ GR  +G+ S+ LL K  +PRPL+M   L    +
Sbjct: 361 RQIGESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSI 420

Query: 426 GQLLIAFPVPGSVYLASIIIGFTFGAQLPLLFAIISELFGLKYFSTLFNFGQIASPLGSY 485
           G L+IA    G++Y+ S+I+G  +G+Q  L+  I SELFG+++  T+FN   +ASP+GSY
Sbjct: 421 GHLIIASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVASPIGSY 480

Query: 486 ILNVQVTGKLYDKQALKQLREKGLDRSAVKELVCLGKQCYRESFSLTAGVTFAGALISLI 545
           I +V++ G +YDK A            + +   C G  C+R SF + A V F G L++++
Sbjct: 481 IFSVRLIGYIYDKTA------------SGEGNTCYGSHCFRLSFIIMASVAFFGFLVAIV 529