MS006874 (gene) Bitter gourd (TR) v1

Overview
NameMS006874
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionProtein NUCLEAR FUSION DEFECTIVE 4-like
Locationscaffold871: 346996 .. 348681 (-)
RNA-Seq ExpressionMS006874
SyntenyMS006874
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCGACAACAACCATGCAGCAGCGAGTTGGCATTTCGTGAAACAAGTTGCAGAAGGGCGATGGTTTTCAGTATTCGCATCGTTTCTGATCATGATCGGCGCCGGTTCAACTTACCTCTTCGGAACATACTCAAAACTCTTGAAGACCCAATTCGATTACGACCAAACCCAAGTTAACACCTTGGGATTTGCCAAAGATCTCGGCTCCAACTTCGGAGTTTTCGCCGGACTTCTCGCCGAGGTGGCTCCCTCGTGGGTCCTTTTCCTCGTCGGCTCATCCCTCAACTTCTTCAGCTACTTCATGATTTGGCTCTCCGTCACCCGCCGCATTGCCAACCCCCAATTCTGGCACATGTGCCTCTACATTTGCTTGGCTGCAAATTCCCAGAATTTCGCCAACACTGCAGTCCTCGTCACGAGCGTCAAAAACTTTCCGGAGCGACGAGGGATCATCCTGGGGCTCCTCAAGGGCTTTGTCGGGATCGGAGGCGCCATCTTGACGCAGATGTATTTAGCCATTTACGGCCACGAAAACCCGGCCAGTGTGATTCTCCTTCTAGCGTGGTTCCCCTCGGTCATATCCTTTGTGTTTGTGCTCTCCATTCGAACCATCAAAGTCCGGAAACACCCGGAGGAGCTCAAAGTGTTTTACCATTTGCTTTATGTCTCCATAATTCTGGCGCTCTATATCTTGTTCTTGACCTTAACCCAAAAGCAGGCTGTTTTCTCCCACGCTGGCTACGTGGGTGGCGCCTCCGTGATTGTGTGCTTGTTGTGTCTCCCCCTTTTGATTGCCATTAGAGAAGAATTCATGCTGTTCAATCTCAACAAACAAAACCAGGACGGACACGTCGCTGTTTTGATCCATGACCAAAAACCTCCGCCCAAAAGGGCATTGGAGGAGGACGCTATAGACAGTGTTTCAATCAAGCCGGAAAGAGGAGATGACTTCACCATCTTTCAAGCTCTCCTCAGTAAAGACATGGCTCTTCTTTTCATAGCTACACTTTCGGGATGTGGCTCGTCTATTGCCGCCATTGACAATATAGGCCAAATCGCGGAGTCGCTTCAATACCCGCCTCACTCCATAGCAATTTTCGTCTCCTGGGTCTCCATATTCAATTTCTTCGGCCGCGTCTTCTCCGGCTTCATCTCCGAAACCCTTATGGTCAAATACAAATTACCTCGCCCTCTCATGTTCGCCTCCTCCTTTCTCCTTACCTGCATTGGCCAGCTCTTAATCGCCTTCCCATATCGCGGCTCAGTCTACGCCGCTTCCTTGATCATTGGTTTCGGGTTTGGAGCCCAAGTTCCATTGCTATTCGCCATAATCTCTGAGCTCTTCGGCCTCAAACACTACTCAACGCTCTTCAACTGCGGCCAACTCGCAGTCCCGTTCGGGTCTTATATACTAAATGTGGATGTAATAGGCAAGTTGTACGATATGGAAGCCATAAGACAAGGTGGAAAGAAGACTGGCAAGGGACTAACTTGCACTGGAGCTAATTGCTTTAGTAATTCCTTCACAATTTTGGCAGCAGTGACTTTTTGTGGAGCTCTCTCTTCGCTTGTGCTGGCTTATAGGACTCGAGACTTTTACAAAGGAGAAACGTATAAGAAATATAGAGAAGATATGTGGATTCCTAAAGCAGATATGGAATTCTATTGTTTGGATAACAGGAAA

mRNA sequence

ATGGGCGACAACAACCATGCAGCAGCGAGTTGGCATTTCGTGAAACAAGTTGCAGAAGGGCGATGGTTTTCAGTATTCGCATCGTTTCTGATCATGATCGGCGCCGGTTCAACTTACCTCTTCGGAACATACTCAAAACTCTTGAAGACCCAATTCGATTACGACCAAACCCAAGTTAACACCTTGGGATTTGCCAAAGATCTCGGCTCCAACTTCGGAGTTTTCGCCGGACTTCTCGCCGAGGTGGCTCCCTCGTGGGTCCTTTTCCTCGTCGGCTCATCCCTCAACTTCTTCAGCTACTTCATGATTTGGCTCTCCGTCACCCGCCGCATTGCCAACCCCCAATTCTGGCACATGTGCCTCTACATTTGCTTGGCTGCAAATTCCCAGAATTTCGCCAACACTGCAGTCCTCGTCACGAGCGTCAAAAACTTTCCGGAGCGACGAGGGATCATCCTGGGGCTCCTCAAGGGCTTTGTCGGGATCGGAGGCGCCATCTTGACGCAGATGTATTTAGCCATTTACGGCCACGAAAACCCGGCCAGTGTGATTCTCCTTCTAGCGTGGTTCCCCTCGGTCATATCCTTTGTGTTTGTGCTCTCCATTCGAACCATCAAAGTCCGGAAACACCCGGAGGAGCTCAAAGTGTTTTACCATTTGCTTTATGTCTCCATAATTCTGGCGCTCTATATCTTGTTCTTGACCTTAACCCAAAAGCAGGCTGTTTTCTCCCACGCTGGCTACGTGGGTGGCGCCTCCGTGATTGTGTGCTTGTTGTGTCTCCCCCTTTTGATTGCCATTAGAGAAGAATTCATGCTGTTCAATCTCAACAAACAAAACCAGGACGGACACGTCGCTGTTTTGATCCATGACCAAAAACCTCCGCCCAAAAGGGCATTGGAGGAGGACGCTATAGACAGTGTTTCAATCAAGCCGGAAAGAGGAGATGACTTCACCATCTTTCAAGCTCTCCTCAGTAAAGACATGGCTCTTCTTTTCATAGCTACACTTTCGGGATGTGGCTCGTCTATTGCCGCCATTGACAATATAGGCCAAATCGCGGAGTCGCTTCAATACCCGCCTCACTCCATAGCAATTTTCGTCTCCTGGGTCTCCATATTCAATTTCTTCGGCCGCGTCTTCTCCGGCTTCATCTCCGAAACCCTTATGGTCAAATACAAATTACCTCGCCCTCTCATGTTCGCCTCCTCCTTTCTCCTTACCTGCATTGGCCAGCTCTTAATCGCCTTCCCATATCGCGGCTCAGTCTACGCCGCTTCCTTGATCATTGGTTTCGGGTTTGGAGCCCAAGTTCCATTGCTATTCGCCATAATCTCTGAGCTCTTCGGCCTCAAACACTACTCAACGCTCTTCAACTGCGGCCAACTCGCAGTCCCGTTCGGGTCTTATATACTAAATGTGGATGTAATAGGCAAGTTGTACGATATGGAAGCCATAAGACAAGGTGGAAAGAAGACTGGCAAGGGACTAACTTGCACTGGAGCTAATTGCTTTAGTAATTCCTTCACAATTTTGGCAGCAGTGACTTTTTGTGGAGCTCTCTCTTCGCTTGTGCTGGCTTATAGGACTCGAGACTTTTACAAAGGAGAAACGTATAAGAAATATAGAGAAGATATGTGGATTCCTAAAGCAGATATGGAATTCTATTGTTTGGATAACAGGAAA

Coding sequence (CDS)

ATGGGCGACAACAACCATGCAGCAGCGAGTTGGCATTTCGTGAAACAAGTTGCAGAAGGGCGATGGTTTTCAGTATTCGCATCGTTTCTGATCATGATCGGCGCCGGTTCAACTTACCTCTTCGGAACATACTCAAAACTCTTGAAGACCCAATTCGATTACGACCAAACCCAAGTTAACACCTTGGGATTTGCCAAAGATCTCGGCTCCAACTTCGGAGTTTTCGCCGGACTTCTCGCCGAGGTGGCTCCCTCGTGGGTCCTTTTCCTCGTCGGCTCATCCCTCAACTTCTTCAGCTACTTCATGATTTGGCTCTCCGTCACCCGCCGCATTGCCAACCCCCAATTCTGGCACATGTGCCTCTACATTTGCTTGGCTGCAAATTCCCAGAATTTCGCCAACACTGCAGTCCTCGTCACGAGCGTCAAAAACTTTCCGGAGCGACGAGGGATCATCCTGGGGCTCCTCAAGGGCTTTGTCGGGATCGGAGGCGCCATCTTGACGCAGATGTATTTAGCCATTTACGGCCACGAAAACCCGGCCAGTGTGATTCTCCTTCTAGCGTGGTTCCCCTCGGTCATATCCTTTGTGTTTGTGCTCTCCATTCGAACCATCAAAGTCCGGAAACACCCGGAGGAGCTCAAAGTGTTTTACCATTTGCTTTATGTCTCCATAATTCTGGCGCTCTATATCTTGTTCTTGACCTTAACCCAAAAGCAGGCTGTTTTCTCCCACGCTGGCTACGTGGGTGGCGCCTCCGTGATTGTGTGCTTGTTGTGTCTCCCCCTTTTGATTGCCATTAGAGAAGAATTCATGCTGTTCAATCTCAACAAACAAAACCAGGACGGACACGTCGCTGTTTTGATCCATGACCAAAAACCTCCGCCCAAAAGGGCATTGGAGGAGGACGCTATAGACAGTGTTTCAATCAAGCCGGAAAGAGGAGATGACTTCACCATCTTTCAAGCTCTCCTCAGTAAAGACATGGCTCTTCTTTTCATAGCTACACTTTCGGGATGTGGCTCGTCTATTGCCGCCATTGACAATATAGGCCAAATCGCGGAGTCGCTTCAATACCCGCCTCACTCCATAGCAATTTTCGTCTCCTGGGTCTCCATATTCAATTTCTTCGGCCGCGTCTTCTCCGGCTTCATCTCCGAAACCCTTATGGTCAAATACAAATTACCTCGCCCTCTCATGTTCGCCTCCTCCTTTCTCCTTACCTGCATTGGCCAGCTCTTAATCGCCTTCCCATATCGCGGCTCAGTCTACGCCGCTTCCTTGATCATTGGTTTCGGGTTTGGAGCCCAAGTTCCATTGCTATTCGCCATAATCTCTGAGCTCTTCGGCCTCAAACACTACTCAACGCTCTTCAACTGCGGCCAACTCGCAGTCCCGTTCGGGTCTTATATACTAAATGTGGATGTAATAGGCAAGTTGTACGATATGGAAGCCATAAGACAAGGTGGAAAGAAGACTGGCAAGGGACTAACTTGCACTGGAGCTAATTGCTTTAGTAATTCCTTCACAATTTTGGCAGCAGTGACTTTTTGTGGAGCTCTCTCTTCGCTTGTGCTGGCTTATAGGACTCGAGACTTTTACAAAGGAGAAACGTATAAGAAATATAGAGAAGATATGTGGATTCCTAAAGCAGATATGGAATTCTATTGTTTGGATAACAGGAAA

Protein sequence

MGDNNHAAASWHFVKQVAEGRWFSVFASFLIMIGAGSTYLFGTYSKLLKTQFDYDQTQVNTLGFAKDLGSNFGVFAGLLAEVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMCLYICLAANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHENPASVILLLAWFPSVISFVFVLSIRTIKVRKHPEELKVFYHLLYVSIILALYILFLTLTQKQAVFSHAGYVGGASVIVCLLCLPLLIAIREEFMLFNLNKQNQDGHVAVLIHDQKPPPKRALEEDAIDSVSIKPERGDDFTIFQALLSKDMALLFIATLSGCGSSIAAIDNIGQIAESLQYPPHSIAIFVSWVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLLTCIGQLLIAFPYRGSVYAASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPFGSYILNVDVIGKLYDMEAIRQGGKKTGKGLTCTGANCFSNSFTILAAVTFCGALSSLVLAYRTRDFYKGETYKKYREDMWIPKADMEFYCLDNRK
Homology
BLAST of MS006874 vs. NCBI nr
Match: XP_022152849.1 (uncharacterized protein LOC111020473 [Momordica charantia])

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 560/562 (99.64%), Postives = 560/562 (99.64%), Query Frame = 0

Query: 1   MGDNNHAAASWHFVKQVAEGRWFSVFASFLIMIGAGSTYLFGTYSKLLKTQFDYDQTQVN 60
           MGDNNHAAASWHFVKQVAEGRWFSVFASFLIMIGAGSTYLFGTYSKLLKTQFDYDQTQVN
Sbjct: 1   MGDNNHAAASWHFVKQVAEGRWFSVFASFLIMIGAGSTYLFGTYSKLLKTQFDYDQTQVN 60

Query: 61  TLGFAKDLGSNFGVFAGLLAEVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMC 120
           TLGFAKDLGSNFGVFAGLLAEVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMC
Sbjct: 61  TLGFAKDLGSNFGVFAGLLAEVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMC 120

Query: 121 LYICLAANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHENP 180
           LYICLAANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHEN 
Sbjct: 121 LYICLAANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHENQ 180

Query: 181 ASVILLLAWFPSVISFVFVLSIRTIKVRKHPEELKVFYHLLYVSIILALYILFLTLTQKQ 240
           ASVILLLAWFPSVISFVFVLSIRTIKVRKHPEELKVFYHLLYVSIILALYILFLTLTQKQ
Sbjct: 181 ASVILLLAWFPSVISFVFVLSIRTIKVRKHPEELKVFYHLLYVSIILALYILFLTLTQKQ 240

Query: 241 AVFSHAGYVGGASVIVCLLCLPLLIAIREEFMLFNLNKQNQDGHVAVLIHDQKPPPKRAL 300
           AVFSHAGYVGGASVIVCLLCLPLLIAIREEFMLFNLNKQNQDGHVAVLIHDQKPPPKRAL
Sbjct: 241 AVFSHAGYVGGASVIVCLLCLPLLIAIREEFMLFNLNKQNQDGHVAVLIHDQKPPPKRAL 300

Query: 301 EEDAIDSVSIKPERGDDFTIFQALLSKDMALLFIATLSGCGSSIAAIDNIGQIAESLQYP 360
           EEDAIDSVSIKPERGDDFTIFQALLSKDMALLFIATLSGCGSSIAAIDNIGQIAESLQYP
Sbjct: 301 EEDAIDSVSIKPERGDDFTIFQALLSKDMALLFIATLSGCGSSIAAIDNIGQIAESLQYP 360

Query: 361 PHSIAIFVSWVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLLTCIGQLLIAFPYR 420
           PHSIAIFVSWVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLLTCIGQLLIAFPYR
Sbjct: 361 PHSIAIFVSWVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLLTCIGQLLIAFPYR 420

Query: 421 GSVYAASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPFGSYILNVDVIGKL 480
           GSVYAASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPFGSYILNVDVIGKL
Sbjct: 421 GSVYAASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPFGSYILNVDVIGKL 480

Query: 481 YDMEAIRQGGKKTGKGLTCTGANCFSNSFTILAAVTFCGALSSLVLAYRTRDFYKGETYK 540
           YDMEAIRQGGKKTGKGLTCTGANCFSNSFTILAAVTF GALSSLVLAYRTRDFYKGETYK
Sbjct: 481 YDMEAIRQGGKKTGKGLTCTGANCFSNSFTILAAVTFFGALSSLVLAYRTRDFYKGETYK 540

Query: 541 KYREDMWIPKADMEFYCLDNRK 563
           KYREDMWIPKADMEFYCLDNRK
Sbjct: 541 KYREDMWIPKADMEFYCLDNRK 562

BLAST of MS006874 vs. NCBI nr
Match: XP_022152845.1 (uncharacterized protein LOC111020469 [Momordica charantia])

HSP 1 Score: 839.0 bits (2166), Expect = 2.5e-239
Identity = 420/563 (74.60%), Postives = 479/563 (85.08%), Query Frame = 0

Query: 5   NHAAASWHFVKQVAEGRWFSVFASFLIMIGAGSTYLFGTYSKLLKTQFDYDQTQVNTLGF 64
           N A  S +FV+QV EGRWFSVFASFLIMIGAGSTY+FGTYSK++K QFDY QTQVNTLGF
Sbjct: 4   NRAEQSLYFVRQVVEGRWFSVFASFLIMIGAGSTYVFGTYSKVMKAQFDYSQTQVNTLGF 63

Query: 65  AKDLGSNFGVFAGLLAEVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMCLYIC 124
            KDLGSN GVFAGLLAEVAP WVLF+VGSSLNF SYFMIWLS+T RIA P+FWHMC YIC
Sbjct: 64  FKDLGSNLGVFAGLLAEVAPPWVLFVVGSSLNFVSYFMIWLSLTHRIAKPEFWHMCTYIC 123

Query: 125 LAANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHENPASVI 184
           LAANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVG+GGAILTQMYLAIYGHEN  +++
Sbjct: 124 LAANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVGVGGAILTQMYLAIYGHENQVNLV 183

Query: 185 LLLAWFPSVISFVFVLSIRTIKVRKHPEELKVFYHLLYVSIILALYILFLTLTQKQAVFS 244
           LLL+WFP++IS VF+L+IR IKVRKHP+EL+V YHLLYVSIILAL++LFLT+TQKQAVFS
Sbjct: 184 LLLSWFPTIISLVFLLTIRPIKVRKHPDELRVMYHLLYVSIILALFLLFLTITQKQAVFS 243

Query: 245 HAGYVGGASVIVCLLCLPLLIAIREEFMLFNLNKQNQDGHVAVLIHDQKPPPKR-----A 304
             GY+ GA+VI  LL +PLLIA+REEF+LF LNK+  +  V V I DQ+ P         
Sbjct: 244 QEGYMSGAAVIASLLSIPLLIAVREEFLLFKLNKETTNPSVTVSILDQQRPAPMLDEIVE 303

Query: 305 LEEDAIDSVSIKPERGDDFTIFQALLSKDMALLFIATLSGCGSSIAAIDNIGQIAESLQY 364
            E   + SV  KP+RGDD+TI QAL SKDMAL+F+  L GCGSSIAAIDN+GQI ESL Y
Sbjct: 304 AEPSCMSSVFNKPQRGDDYTILQALFSKDMALIFVGALCGCGSSIAAIDNLGQIGESLYY 363

Query: 365 PPHSIAIFVSWVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLLTCIGQLLIAFPY 424
           P  SI IF+S +SIFNFFGRV SGFISETLM K+KLPRPLMFA + LLTCIG L +AFP+
Sbjct: 364 PAQSITIFISLISIFNFFGRVCSGFISETLMTKHKLPRPLMFALAHLLTCIGLLFVAFPF 423

Query: 425 RGSVYAASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPFGSYILNVDVIGK 484
            GS+Y AS+++GFGFGAQVP++FAIISELFGLK Y+TLFN GQL+VP GSYILNVD+IGK
Sbjct: 424 HGSIYIASVVVGFGFGAQVPMVFAIISELFGLKRYATLFNFGQLSVPIGSYILNVDIIGK 483

Query: 485 LYDMEAIRQGGKKTGKGLTCTGANCFSNSFTILAAVTFCGALSSLVLAYRTRDFYKGETY 544
           LYDMEA +QG  K GKGLTCTG  CFS SFTILA VT  GAL+SLVLA+RTRDFYKG+ Y
Sbjct: 484 LYDMEARKQGTTKNGKGLTCTGHECFSTSFTILATVTLFGALTSLVLAFRTRDFYKGDVY 543

Query: 545 KKYREDMWIPKADMEFYCLDNRK 563
            KYREDMW+P++DMEFYCLDN+K
Sbjct: 544 NKYREDMWMPESDMEFYCLDNKK 566

BLAST of MS006874 vs. NCBI nr
Match: XP_023521059.1 (uncharacterized protein LOC111784657 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 837.4 bits (2162), Expect = 7.2e-239
Identity = 420/569 (73.81%), Postives = 486/569 (85.41%), Query Frame = 0

Query: 4   NNHAAASWHFVKQVAEGRWFSVFASFLIMIGAGSTYLFGTYSKLLKTQFDYDQTQVNTLG 63
           +N    SW F+KQV EGRWF+VFASFLIMIGAGSTY+FGTYSK +K+QFDY+QTQ+NTLG
Sbjct: 3   DNSLPESWRFIKQVVEGRWFAVFASFLIMIGAGSTYIFGTYSKAIKSQFDYNQTQINTLG 62

Query: 64  FAKDLGSNFGVFAGLLAEVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMCLYI 123
           FAKDLGSNFGVFAGLL EVAP WVLFLVGS+LNF+SYFMIWLS+T RIA P FW M L+I
Sbjct: 63  FAKDLGSNFGVFAGLLGEVAPPWVLFLVGSALNFWSYFMIWLSITGRIAKPPFWEMFLFI 122

Query: 124 CLAANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHENPASV 183
           C+AANSQNFANTA+LVTSV+NFP+RRGIILGLLKGFVG GGAILTQ YLA+YGH+NP ++
Sbjct: 123 CVAANSQNFANTAILVTSVRNFPDRRGIILGLLKGFVGFGGAILTQFYLALYGHDNPTNL 182

Query: 184 ILLLAWFPSVISFVFVLSIRTIKVRKHPEELKVFYHLLYVSIILALYILFLTLTQKQAVF 243
           +LLL+W P+V+S +F LSIR IKVRKHP+EL+V YHLLYVSIILAL++LFLT+TQKQ VF
Sbjct: 183 VLLLSWLPTVVSMLFFLSIRPIKVRKHPDELRVLYHLLYVSIILALFLLFLTITQKQTVF 242

Query: 244 SHAGYVGGASVIVCLLCLPLLIAIREEFMLFNLNKQ--NQDGHVAVLIHDQKPPPK---- 303
           +   Y+ GA+VI+ LL +PLLIAIREEFMLF LNKQ  N +  V +L+ + +P PK    
Sbjct: 243 TQEAYMSGATVIIGLLLIPLLIAIREEFMLFKLNKQTLNNNPTVPILMPEHEPSPKAPPT 302

Query: 304 -RALEEDAIDSVSI---KPERGDDFTIFQALLSKDMALLFIATLSGCGSSIAAIDNIGQI 363
            + + E +    S+   KP+RG+DFTI QAL SKDMAL+FIATL+ CG+SIAAIDN+GQ+
Sbjct: 303 IQQIPEASTSCFSMAFKKPQRGEDFTILQALFSKDMALVFIATLTACGASIAAIDNLGQV 362

Query: 364 AESLQYPPHSIAIFVSWVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLLTCIGQL 423
            ESL YP  SI+IFVSWVSIFNFFGRVFSGFISE LM KYKLPRPLMFA S LLTCIG L
Sbjct: 363 GESLGYPSQSISIFVSWVSIFNFFGRVFSGFISEALMTKYKLPRPLMFAFSHLLTCIGIL 422

Query: 424 LIAFPYRGSVYAASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPFGSYILN 483
            IAFPY+GSVYAASLIIGFGFGAQVP++FAIISELFGLK+YST+FNCGQLAVP GSYILN
Sbjct: 423 FIAFPYKGSVYAASLIIGFGFGAQVPMVFAIISELFGLKYYSTIFNCGQLAVPIGSYILN 482

Query: 484 VDVIGKLYDMEAIRQGGKKTGKGLTCTGANCFSNSFTILAAVTFCGALSSLVLAYRTRDF 543
           VDVIGKLYD+EA + GG K GKGLTCTG  CFS SF ILA V   GA+ SLVLAYRT+DF
Sbjct: 483 VDVIGKLYDIEAAKLGGVKNGKGLTCTGTPCFSGSFLILAVVVLFGAVVSLVLAYRTQDF 542

Query: 544 YKGETYKKYREDMWIPKADMEFYCLDNRK 563
           YKG+ Y KYREDMWIP++DMEFYC+D+RK
Sbjct: 543 YKGDVYNKYREDMWIPQSDMEFYCIDDRK 571

BLAST of MS006874 vs. NCBI nr
Match: KAG6583549.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 833.2 bits (2151), Expect = 1.4e-237
Identity = 418/569 (73.46%), Postives = 486/569 (85.41%), Query Frame = 0

Query: 4   NNHAAASWHFVKQVAEGRWFSVFASFLIMIGAGSTYLFGTYSKLLKTQFDYDQTQVNTLG 63
           +N    SW F+KQV EGRWF+VFASFLIMIGAGSTY+FGTYSK +K+QFDY+QTQ+NTLG
Sbjct: 3   DNSLPESWRFIKQVVEGRWFAVFASFLIMIGAGSTYIFGTYSKAIKSQFDYNQTQINTLG 62

Query: 64  FAKDLGSNFGVFAGLLAEVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMCLYI 123
           FAKDLGSNFGVFAGLL EVAP WVLFLVGS+LNF+SYFMIWLS+T RI+ P FW M ++I
Sbjct: 63  FAKDLGSNFGVFAGLLGEVAPPWVLFLVGSALNFWSYFMIWLSITGRISKPPFWEMFVFI 122

Query: 124 CLAANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHENPASV 183
           C+AANSQNFANTAVLVTSV+NFP+RRGIILGLLKGFVG GGAILTQ YLA+YGH+NP ++
Sbjct: 123 CVAANSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGFGGAILTQFYLALYGHDNPTNL 182

Query: 184 ILLLAWFPSVISFVFVLSIRTIKVRKHPEELKVFYHLLYVSIILALYILFLTLTQKQAVF 243
           +LLL+W P+V S +F LSIR IKVRK P+EL+V YHLLYVSIILAL++LFLT+TQKQ VF
Sbjct: 183 VLLLSWLPTVASMLFFLSIRPIKVRKDPDELRVLYHLLYVSIILALFLLFLTITQKQTVF 242

Query: 244 SHAGYVGGASVIVCLLCLPLLIAIREEFMLFNLNKQ--NQDGHVAVLIHDQKPPPK---- 303
           +   Y+ GA+VI+ LL +PLLIAIREEFMLF LNKQ  N +  V +L+ + +P PK    
Sbjct: 243 TQEAYMSGATVIIGLLLIPLLIAIREEFMLFKLNKQTLNNNPTVPILMPEHEPSPKAPPT 302

Query: 304 -RALEEDAIDSVSI---KPERGDDFTIFQALLSKDMALLFIATLSGCGSSIAAIDNIGQI 363
            + + E +    S+   KP+RG+DFTI QAL SKDMAL+FIATL+ CG+SIAAIDN+GQ+
Sbjct: 303 IQKIPEASTSCFSMTFKKPQRGEDFTILQALFSKDMALVFIATLTACGASIAAIDNLGQV 362

Query: 364 AESLQYPPHSIAIFVSWVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLLTCIGQL 423
            ESL YP  +I+IFVSWVSIFNFFGRVFSGFISE LM KYKLPRPLMFA S LLTCIG L
Sbjct: 363 GESLGYPSQTISIFVSWVSIFNFFGRVFSGFISEALMTKYKLPRPLMFAFSHLLTCIGIL 422

Query: 424 LIAFPYRGSVYAASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPFGSYILN 483
            IAFPY+GSVYAASLIIGFGFGAQVP+LFAIISELFGLK+YST+FNCGQLAVP GSYILN
Sbjct: 423 FIAFPYKGSVYAASLIIGFGFGAQVPMLFAIISELFGLKYYSTIFNCGQLAVPIGSYILN 482

Query: 484 VDVIGKLYDMEAIRQGGKKTGKGLTCTGANCFSNSFTILAAVTFCGALSSLVLAYRTRDF 543
           VDVIGKLYD+EA + GG K GKGLTCTG +CFS SF ILA V   GA++SLVLAYRTRDF
Sbjct: 483 VDVIGKLYDIEAAKLGGVKNGKGLTCTGTHCFSGSFLILAVVVLFGAVASLVLAYRTRDF 542

Query: 544 YKGETYKKYREDMWIPKADMEFYCLDNRK 563
           YKG+ Y +YREDMWIP++DMEFYC+D+RK
Sbjct: 543 YKGDVYNRYREDMWIPQSDMEFYCIDDRK 571

BLAST of MS006874 vs. NCBI nr
Match: XP_022964772.1 (uncharacterized protein LOC111464772 [Cucurbita moschata])

HSP 1 Score: 832.8 bits (2150), Expect = 1.8e-237
Identity = 418/569 (73.46%), Postives = 485/569 (85.24%), Query Frame = 0

Query: 4   NNHAAASWHFVKQVAEGRWFSVFASFLIMIGAGSTYLFGTYSKLLKTQFDYDQTQVNTLG 63
           +N    SW F+KQV EGRWF+VFASFLIMIGAGSTY+FGTYSK +K+QFDY+QTQ+NTLG
Sbjct: 3   DNSLPESWRFIKQVVEGRWFAVFASFLIMIGAGSTYIFGTYSKAIKSQFDYNQTQINTLG 62

Query: 64  FAKDLGSNFGVFAGLLAEVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMCLYI 123
           FAKDLGSNFGVFAGLL EVAP WVLFLVGS+LNF+SYFMIWLS+T RIA P FW M L+I
Sbjct: 63  FAKDLGSNFGVFAGLLGEVAPPWVLFLVGSALNFWSYFMIWLSITGRIAKPPFWEMFLFI 122

Query: 124 CLAANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHENPASV 183
           C+AANSQNFANTA+LVTSV+NFP+RRGIILGLLKGFVG GGAILTQ YLA+YGH+NP ++
Sbjct: 123 CVAANSQNFANTAILVTSVRNFPDRRGIILGLLKGFVGFGGAILTQFYLALYGHDNPTNL 182

Query: 184 ILLLAWFPSVISFVFVLSIRTIKVRKHPEELKVFYHLLYVSIILALYILFLTLTQKQAVF 243
           +LLL+W P+V S +F LSIR IKVRK P+EL+V YHLLYVSIILAL++LFLT+TQKQ VF
Sbjct: 183 VLLLSWLPTVASMLFFLSIRPIKVRKDPDELRVLYHLLYVSIILALFLLFLTITQKQTVF 242

Query: 244 SHAGYVGGASVIVCLLCLPLLIAIREEFMLFNLNKQ--NQDGHVAVLIHDQKPPPK---- 303
           +   Y+ GA+VI+ LL +PLLIAIREEFMLF LNKQ  N +  V +L+ + +P PK    
Sbjct: 243 TQEAYMSGATVIIGLLLIPLLIAIREEFMLFKLNKQTLNNNPTVPILMPEHEPSPKAPPT 302

Query: 304 -RALEEDAIDSVSI---KPERGDDFTIFQALLSKDMALLFIATLSGCGSSIAAIDNIGQI 363
            + + E +    S+   KP+RG+DFTI QAL SKDMAL+FIATL+ CG+SIAAIDN+GQ+
Sbjct: 303 IQQIPEASTSCFSMTFKKPQRGEDFTILQALFSKDMALVFIATLTACGASIAAIDNLGQV 362

Query: 364 AESLQYPPHSIAIFVSWVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLLTCIGQL 423
            ESL YP  +I+IFVSWVSIFNFFGRVFSGFISE LM KYKLPRPLMFA S LLTCIG L
Sbjct: 363 GESLGYPSQTISIFVSWVSIFNFFGRVFSGFISEALMTKYKLPRPLMFAFSHLLTCIGIL 422

Query: 424 LIAFPYRGSVYAASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPFGSYILN 483
            IAFPY+GSVYAASLIIGFGFGAQVP++FAIISELFGLK+YST+FNCGQLAVP GSYILN
Sbjct: 423 FIAFPYKGSVYAASLIIGFGFGAQVPMVFAIISELFGLKYYSTIFNCGQLAVPIGSYILN 482

Query: 484 VDVIGKLYDMEAIRQGGKKTGKGLTCTGANCFSNSFTILAAVTFCGALSSLVLAYRTRDF 543
           VDVIGKLYD+EA + GG K GKGLTCTG +CFS SF ILA V   GA+ SLVLAYRT+DF
Sbjct: 483 VDVIGKLYDIEAAKLGGVKNGKGLTCTGTHCFSGSFLILAVVVLLGAVVSLVLAYRTQDF 542

Query: 544 YKGETYKKYREDMWIPKADMEFYCLDNRK 563
           YKG+ Y KYREDMWIP++DMEFYC+D+RK
Sbjct: 543 YKGDVYNKYREDMWIPQSDMEFYCIDDRK 571

BLAST of MS006874 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 7.9e-31
Identity = 138/532 (25.94%), Postives = 238/532 (44.74%), Query Frame = 0

Query: 21  RWFSVFASFLIMIGAGSTYLFGTYSKLLKTQFDYDQTQVNTLGFAKDLGSNFGVFAGLLA 80
           +W  + A+  I    G+ + F  YS  LK+     Q ++N L  A DLG  FG  +G+  
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 81  EVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMCLYIC--LAANSQNFANTAVL 140
              P  V+    +++ F  Y + WL +T  I  P   +  +++C  LA  S  + NTA  
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP---YSLVFLCCLLAGLSICWFNTACF 162

Query: 141 VTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHENPAS---VILLLAWFPSVIS 200
           +  +++FP  R + L L   F GI  A+ +  + AI    NP+S    +LL +  P V+S
Sbjct: 163 ILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAI----NPSSSNLYLLLNSLVPLVVS 222

Query: 201 FVF---VLSIRTIKVRKHPEELKVFYHLLYVSIILALYILF-LTLTQKQAVFSHAGYVGG 260
           F     VL+  ++      +  +   H+  +  +LA+   F L L+      +   ++G 
Sbjct: 223 FAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIGA 282

Query: 261 ASVIVCLLCLPLLIAIREEFM-LFNLNKQNQ-DGHVAVLIHDQKPPPKRALEEDAIDSVS 320
             ++V  LC PLL+  R+ F+ + N    ++  G+V + I + K        +   + + 
Sbjct: 283 VVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHMG 342

Query: 321 IKPE----RGDDFTIFQALLSK-DMALLFIATLSGCGSSIAAIDNIGQIAESLQYPPHSI 380
              E    R  D   F+ L+S+ +  L +IA   G    +   +N+GQIA+SL     + 
Sbjct: 343 TAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSL---GQNS 402

Query: 381 AIFVSWVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLLTCIGQLLIAF--PYRGS 440
              V+  S F+FFGR+ S    + +  +++L R   FA + L T I   L+A     + +
Sbjct: 403 TTLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTA 462

Query: 441 VYAASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPFGSYILNVDVIGKLYD 500
           +  A+ +IG   G       +I S+LFG        N     +P GS +    +   +Y+
Sbjct: 463 LQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGY-IAASIYE 522

Query: 501 MEAIRQGGKKTGKGLTCTGANCFSNSFTILAAVTFCGALSSLVLAYRTRDFY 535
             A           + C G +C+  +F     ++  G +SSL L  RT+  Y
Sbjct: 523 ANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of MS006874 vs. ExPASy TrEMBL
Match: A0A6J1DHB0 (uncharacterized protein LOC111020473 OS=Momordica charantia OX=3673 GN=LOC111020473 PE=4 SV=1)

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 560/562 (99.64%), Postives = 560/562 (99.64%), Query Frame = 0

Query: 1   MGDNNHAAASWHFVKQVAEGRWFSVFASFLIMIGAGSTYLFGTYSKLLKTQFDYDQTQVN 60
           MGDNNHAAASWHFVKQVAEGRWFSVFASFLIMIGAGSTYLFGTYSKLLKTQFDYDQTQVN
Sbjct: 1   MGDNNHAAASWHFVKQVAEGRWFSVFASFLIMIGAGSTYLFGTYSKLLKTQFDYDQTQVN 60

Query: 61  TLGFAKDLGSNFGVFAGLLAEVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMC 120
           TLGFAKDLGSNFGVFAGLLAEVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMC
Sbjct: 61  TLGFAKDLGSNFGVFAGLLAEVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMC 120

Query: 121 LYICLAANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHENP 180
           LYICLAANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHEN 
Sbjct: 121 LYICLAANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHENQ 180

Query: 181 ASVILLLAWFPSVISFVFVLSIRTIKVRKHPEELKVFYHLLYVSIILALYILFLTLTQKQ 240
           ASVILLLAWFPSVISFVFVLSIRTIKVRKHPEELKVFYHLLYVSIILALYILFLTLTQKQ
Sbjct: 181 ASVILLLAWFPSVISFVFVLSIRTIKVRKHPEELKVFYHLLYVSIILALYILFLTLTQKQ 240

Query: 241 AVFSHAGYVGGASVIVCLLCLPLLIAIREEFMLFNLNKQNQDGHVAVLIHDQKPPPKRAL 300
           AVFSHAGYVGGASVIVCLLCLPLLIAIREEFMLFNLNKQNQDGHVAVLIHDQKPPPKRAL
Sbjct: 241 AVFSHAGYVGGASVIVCLLCLPLLIAIREEFMLFNLNKQNQDGHVAVLIHDQKPPPKRAL 300

Query: 301 EEDAIDSVSIKPERGDDFTIFQALLSKDMALLFIATLSGCGSSIAAIDNIGQIAESLQYP 360
           EEDAIDSVSIKPERGDDFTIFQALLSKDMALLFIATLSGCGSSIAAIDNIGQIAESLQYP
Sbjct: 301 EEDAIDSVSIKPERGDDFTIFQALLSKDMALLFIATLSGCGSSIAAIDNIGQIAESLQYP 360

Query: 361 PHSIAIFVSWVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLLTCIGQLLIAFPYR 420
           PHSIAIFVSWVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLLTCIGQLLIAFPYR
Sbjct: 361 PHSIAIFVSWVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLLTCIGQLLIAFPYR 420

Query: 421 GSVYAASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPFGSYILNVDVIGKL 480
           GSVYAASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPFGSYILNVDVIGKL
Sbjct: 421 GSVYAASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPFGSYILNVDVIGKL 480

Query: 481 YDMEAIRQGGKKTGKGLTCTGANCFSNSFTILAAVTFCGALSSLVLAYRTRDFYKGETYK 540
           YDMEAIRQGGKKTGKGLTCTGANCFSNSFTILAAVTF GALSSLVLAYRTRDFYKGETYK
Sbjct: 481 YDMEAIRQGGKKTGKGLTCTGANCFSNSFTILAAVTFFGALSSLVLAYRTRDFYKGETYK 540

Query: 541 KYREDMWIPKADMEFYCLDNRK 563
           KYREDMWIPKADMEFYCLDNRK
Sbjct: 541 KYREDMWIPKADMEFYCLDNRK 562

BLAST of MS006874 vs. ExPASy TrEMBL
Match: A0A6J1DF43 (uncharacterized protein LOC111020469 OS=Momordica charantia OX=3673 GN=LOC111020469 PE=4 SV=1)

HSP 1 Score: 839.0 bits (2166), Expect = 1.2e-239
Identity = 420/563 (74.60%), Postives = 479/563 (85.08%), Query Frame = 0

Query: 5   NHAAASWHFVKQVAEGRWFSVFASFLIMIGAGSTYLFGTYSKLLKTQFDYDQTQVNTLGF 64
           N A  S +FV+QV EGRWFSVFASFLIMIGAGSTY+FGTYSK++K QFDY QTQVNTLGF
Sbjct: 4   NRAEQSLYFVRQVVEGRWFSVFASFLIMIGAGSTYVFGTYSKVMKAQFDYSQTQVNTLGF 63

Query: 65  AKDLGSNFGVFAGLLAEVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMCLYIC 124
            KDLGSN GVFAGLLAEVAP WVLF+VGSSLNF SYFMIWLS+T RIA P+FWHMC YIC
Sbjct: 64  FKDLGSNLGVFAGLLAEVAPPWVLFVVGSSLNFVSYFMIWLSLTHRIAKPEFWHMCTYIC 123

Query: 125 LAANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHENPASVI 184
           LAANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVG+GGAILTQMYLAIYGHEN  +++
Sbjct: 124 LAANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVGVGGAILTQMYLAIYGHENQVNLV 183

Query: 185 LLLAWFPSVISFVFVLSIRTIKVRKHPEELKVFYHLLYVSIILALYILFLTLTQKQAVFS 244
           LLL+WFP++IS VF+L+IR IKVRKHP+EL+V YHLLYVSIILAL++LFLT+TQKQAVFS
Sbjct: 184 LLLSWFPTIISLVFLLTIRPIKVRKHPDELRVMYHLLYVSIILALFLLFLTITQKQAVFS 243

Query: 245 HAGYVGGASVIVCLLCLPLLIAIREEFMLFNLNKQNQDGHVAVLIHDQKPPPKR-----A 304
             GY+ GA+VI  LL +PLLIA+REEF+LF LNK+  +  V V I DQ+ P         
Sbjct: 244 QEGYMSGAAVIASLLSIPLLIAVREEFLLFKLNKETTNPSVTVSILDQQRPAPMLDEIVE 303

Query: 305 LEEDAIDSVSIKPERGDDFTIFQALLSKDMALLFIATLSGCGSSIAAIDNIGQIAESLQY 364
            E   + SV  KP+RGDD+TI QAL SKDMAL+F+  L GCGSSIAAIDN+GQI ESL Y
Sbjct: 304 AEPSCMSSVFNKPQRGDDYTILQALFSKDMALIFVGALCGCGSSIAAIDNLGQIGESLYY 363

Query: 365 PPHSIAIFVSWVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLLTCIGQLLIAFPY 424
           P  SI IF+S +SIFNFFGRV SGFISETLM K+KLPRPLMFA + LLTCIG L +AFP+
Sbjct: 364 PAQSITIFISLISIFNFFGRVCSGFISETLMTKHKLPRPLMFALAHLLTCIGLLFVAFPF 423

Query: 425 RGSVYAASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPFGSYILNVDVIGK 484
            GS+Y AS+++GFGFGAQVP++FAIISELFGLK Y+TLFN GQL+VP GSYILNVD+IGK
Sbjct: 424 HGSIYIASVVVGFGFGAQVPMVFAIISELFGLKRYATLFNFGQLSVPIGSYILNVDIIGK 483

Query: 485 LYDMEAIRQGGKKTGKGLTCTGANCFSNSFTILAAVTFCGALSSLVLAYRTRDFYKGETY 544
           LYDMEA +QG  K GKGLTCTG  CFS SFTILA VT  GAL+SLVLA+RTRDFYKG+ Y
Sbjct: 484 LYDMEARKQGTTKNGKGLTCTGHECFSTSFTILATVTLFGALTSLVLAFRTRDFYKGDVY 543

Query: 545 KKYREDMWIPKADMEFYCLDNRK 563
            KYREDMW+P++DMEFYCLDN+K
Sbjct: 544 NKYREDMWMPESDMEFYCLDNKK 566

BLAST of MS006874 vs. ExPASy TrEMBL
Match: A0A6J1I3B9 (uncharacterized protein LOC111469246 OS=Cucurbita maxima OX=3661 GN=LOC111469246 PE=4 SV=1)

HSP 1 Score: 832.8 bits (2150), Expect = 8.6e-238
Identity = 416/569 (73.11%), Postives = 483/569 (84.89%), Query Frame = 0

Query: 4   NNHAAASWHFVKQVAEGRWFSVFASFLIMIGAGSTYLFGTYSKLLKTQFDYDQTQVNTLG 63
           +N    SW F+KQV EGRWF+VFASFLIMIGAGSTY+FGTYSK +K+QF+Y+QTQ+NTLG
Sbjct: 3   DNSLPESWRFIKQVVEGRWFAVFASFLIMIGAGSTYIFGTYSKAIKSQFNYNQTQINTLG 62

Query: 64  FAKDLGSNFGVFAGLLAEVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMCLYI 123
           FAKDLGSNFGVFAGLL EVAP WVLFLVGS+LNF+SYFMIWLS+T RIA P FW M L+I
Sbjct: 63  FAKDLGSNFGVFAGLLGEVAPPWVLFLVGSALNFWSYFMIWLSITGRIAKPPFWEMFLFI 122

Query: 124 CLAANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHENPASV 183
           C+AANSQNFANTA+LVTSV+NFP+RRGIILGLLKGFVG GGAILTQ YLA+YGH+NP ++
Sbjct: 123 CVAANSQNFANTAILVTSVRNFPDRRGIILGLLKGFVGFGGAILTQFYLALYGHDNPTNL 182

Query: 184 ILLLAWFPSVISFVFVLSIRTIKVRKHPEELKVFYHLLYVSIILALYILFLTLTQKQAVF 243
           +LLL+W P+V+S +F LSIR IKVRKHP+EL+V YHLLYVSIILAL++LFLT+TQKQ VF
Sbjct: 183 VLLLSWLPTVVSMLFFLSIRPIKVRKHPDELRVLYHLLYVSIILALFLLFLTITQKQTVF 242

Query: 244 SHAGYVGGASVIVCLLCLPLLIAIREEFMLFNLNKQ--NQDGHVAVLIHDQKPPPKRALE 303
           +   Y+ GA+VI+ LL +PLLIAIREEF+LF LNKQ  N +  V +L+ + +P PK    
Sbjct: 243 TQEAYMSGATVIIGLLLIPLLIAIREEFLLFKLNKQTLNNNPTVPILMPEHEPSPKAPPT 302

Query: 304 EDAIDSVSI--------KPERGDDFTIFQALLSKDMALLFIATLSGCGSSIAAIDNIGQI 363
            + I   S         +P+RG+DFTI QAL SKDMAL+FIATLS CG+SIAAIDN+GQ+
Sbjct: 303 IEQIPEASTSCFSMAFKRPQRGEDFTILQALFSKDMALVFIATLSACGASIAAIDNLGQV 362

Query: 364 AESLQYPPHSIAIFVSWVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLLTCIGQL 423
            ESL YP  +I+IFVSWVSIFNFFGRVFSGFISE LM KYKLPRPLMFA S LLTCIG L
Sbjct: 363 GESLGYPSQTISIFVSWVSIFNFFGRVFSGFISEALMTKYKLPRPLMFAFSHLLTCIGIL 422

Query: 424 LIAFPYRGSVYAASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPFGSYILN 483
            IAFPY+GSVYAASLIIGFGFGAQVP++FAIISELFGLK+YST+FNCGQLAVP GSYILN
Sbjct: 423 FIAFPYKGSVYAASLIIGFGFGAQVPMVFAIISELFGLKYYSTIFNCGQLAVPIGSYILN 482

Query: 484 VDVIGKLYDMEAIRQGGKKTGKGLTCTGANCFSNSFTILAAVTFCGALSSLVLAYRTRDF 543
           VDVIGKLYD+EA + GG K GKGLTCTG  CFS SF ILA V   GA+ SLVLAYRT+DF
Sbjct: 483 VDVIGKLYDIEAAKLGGVKNGKGLTCTGTPCFSGSFLILAVVVLFGAVVSLVLAYRTQDF 542

Query: 544 YKGETYKKYREDMWIPKADMEFYCLDNRK 563
           YKG+ Y +YREDMWIP++DMEFYC+D+RK
Sbjct: 543 YKGDVYNRYREDMWIPQSDMEFYCIDDRK 571

BLAST of MS006874 vs. ExPASy TrEMBL
Match: A0A6J1HIM2 (uncharacterized protein LOC111464772 OS=Cucurbita moschata OX=3662 GN=LOC111464772 PE=4 SV=1)

HSP 1 Score: 832.8 bits (2150), Expect = 8.6e-238
Identity = 418/569 (73.46%), Postives = 485/569 (85.24%), Query Frame = 0

Query: 4   NNHAAASWHFVKQVAEGRWFSVFASFLIMIGAGSTYLFGTYSKLLKTQFDYDQTQVNTLG 63
           +N    SW F+KQV EGRWF+VFASFLIMIGAGSTY+FGTYSK +K+QFDY+QTQ+NTLG
Sbjct: 3   DNSLPESWRFIKQVVEGRWFAVFASFLIMIGAGSTYIFGTYSKAIKSQFDYNQTQINTLG 62

Query: 64  FAKDLGSNFGVFAGLLAEVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMCLYI 123
           FAKDLGSNFGVFAGLL EVAP WVLFLVGS+LNF+SYFMIWLS+T RIA P FW M L+I
Sbjct: 63  FAKDLGSNFGVFAGLLGEVAPPWVLFLVGSALNFWSYFMIWLSITGRIAKPPFWEMFLFI 122

Query: 124 CLAANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHENPASV 183
           C+AANSQNFANTA+LVTSV+NFP+RRGIILGLLKGFVG GGAILTQ YLA+YGH+NP ++
Sbjct: 123 CVAANSQNFANTAILVTSVRNFPDRRGIILGLLKGFVGFGGAILTQFYLALYGHDNPTNL 182

Query: 184 ILLLAWFPSVISFVFVLSIRTIKVRKHPEELKVFYHLLYVSIILALYILFLTLTQKQAVF 243
           +LLL+W P+V S +F LSIR IKVRK P+EL+V YHLLYVSIILAL++LFLT+TQKQ VF
Sbjct: 183 VLLLSWLPTVASMLFFLSIRPIKVRKDPDELRVLYHLLYVSIILALFLLFLTITQKQTVF 242

Query: 244 SHAGYVGGASVIVCLLCLPLLIAIREEFMLFNLNKQ--NQDGHVAVLIHDQKPPPK---- 303
           +   Y+ GA+VI+ LL +PLLIAIREEFMLF LNKQ  N +  V +L+ + +P PK    
Sbjct: 243 TQEAYMSGATVIIGLLLIPLLIAIREEFMLFKLNKQTLNNNPTVPILMPEHEPSPKAPPT 302

Query: 304 -RALEEDAIDSVSI---KPERGDDFTIFQALLSKDMALLFIATLSGCGSSIAAIDNIGQI 363
            + + E +    S+   KP+RG+DFTI QAL SKDMAL+FIATL+ CG+SIAAIDN+GQ+
Sbjct: 303 IQQIPEASTSCFSMTFKKPQRGEDFTILQALFSKDMALVFIATLTACGASIAAIDNLGQV 362

Query: 364 AESLQYPPHSIAIFVSWVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLLTCIGQL 423
            ESL YP  +I+IFVSWVSIFNFFGRVFSGFISE LM KYKLPRPLMFA S LLTCIG L
Sbjct: 363 GESLGYPSQTISIFVSWVSIFNFFGRVFSGFISEALMTKYKLPRPLMFAFSHLLTCIGIL 422

Query: 424 LIAFPYRGSVYAASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPFGSYILN 483
            IAFPY+GSVYAASLIIGFGFGAQVP++FAIISELFGLK+YST+FNCGQLAVP GSYILN
Sbjct: 423 FIAFPYKGSVYAASLIIGFGFGAQVPMVFAIISELFGLKYYSTIFNCGQLAVPIGSYILN 482

Query: 484 VDVIGKLYDMEAIRQGGKKTGKGLTCTGANCFSNSFTILAAVTFCGALSSLVLAYRTRDF 543
           VDVIGKLYD+EA + GG K GKGLTCTG +CFS SF ILA V   GA+ SLVLAYRT+DF
Sbjct: 483 VDVIGKLYDIEAAKLGGVKNGKGLTCTGTHCFSGSFLILAVVVLLGAVVSLVLAYRTQDF 542

Query: 544 YKGETYKKYREDMWIPKADMEFYCLDNRK 563
           YKG+ Y KYREDMWIP++DMEFYC+D+RK
Sbjct: 543 YKGDVYNKYREDMWIPQSDMEFYCIDDRK 571

BLAST of MS006874 vs. ExPASy TrEMBL
Match: A0A6J1HYK5 (uncharacterized protein LOC111469251 OS=Cucurbita maxima OX=3661 GN=LOC111469251 PE=4 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 1.1e-237
Identity = 413/569 (72.58%), Postives = 483/569 (84.89%), Query Frame = 0

Query: 4   NNHAAASWHFVKQVAEGRWFSVFASFLIMIGAGSTYLFGTYSKLLKTQFDYDQTQVNTLG 63
           +N    SW F+KQV EGRWF+VFASFLIMIGAGSTY+FGTYSK +K+QFDY+QTQ+NTLG
Sbjct: 3   DNSLPESWRFIKQVVEGRWFAVFASFLIMIGAGSTYIFGTYSKAIKSQFDYNQTQINTLG 62

Query: 64  FAKDLGSNFGVFAGLLAEVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMCLYI 123
           FAKDLGSNFGVFAGLL EVAP WVLFLVGS+LNF+SYFMIWLS+T RI  P FW M ++I
Sbjct: 63  FAKDLGSNFGVFAGLLGEVAPPWVLFLVGSALNFWSYFMIWLSITGRIVKPPFWEMFVFI 122

Query: 124 CLAANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHENPASV 183
           C+AANSQNFANTA+LVTSV+NFP+RRGIILGLLKGFVG GGAILTQ YLA+YGH+NP ++
Sbjct: 123 CVAANSQNFANTAILVTSVRNFPDRRGIILGLLKGFVGFGGAILTQFYLALYGHDNPTNL 182

Query: 184 ILLLAWFPSVISFVFVLSIRTIKVRKHPEELKVFYHLLYVSIILALYILFLTLTQKQAVF 243
           +LLL+W P+V+S +F LSIR IKVRKHP+EL+V YHLLYVSIILAL++LFLT+TQKQ VF
Sbjct: 183 VLLLSWLPTVVSMLFFLSIRPIKVRKHPDELRVLYHLLYVSIILALFLLFLTITQKQTVF 242

Query: 244 SHAGYVGGASVIVCLLCLPLLIAIREEFMLFNLNKQ--NQDGHVAVLIHDQKPPPKRALE 303
           +   Y+ GA+VI+ LL +PLLIAIREEF+LF LNKQ  N +  V +L+ + +P PK    
Sbjct: 243 TQEAYMSGATVIIGLLLIPLLIAIREEFLLFKLNKQTLNNNPTVPILMPEHEPSPKAPPT 302

Query: 304 EDAIDSVSI--------KPERGDDFTIFQALLSKDMALLFIATLSGCGSSIAAIDNIGQI 363
            + I   S         +P+RG+DFTI QAL SKDMAL+FIATLS CG+SIAAIDN+GQ+
Sbjct: 303 IEQIPEASTSCFSMAFKRPQRGEDFTILQALFSKDMALVFIATLSACGASIAAIDNLGQV 362

Query: 364 AESLQYPPHSIAIFVSWVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLLTCIGQL 423
            ESL YP  +I+IFVSWVSIFNFFGRVFSGFIS+ LM KYKLPRPLMFA S +LTC+G L
Sbjct: 363 GESLGYPSQTISIFVSWVSIFNFFGRVFSGFISDALMTKYKLPRPLMFAFSHILTCVGIL 422

Query: 424 LIAFPYRGSVYAASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPFGSYILN 483
            IAFPY+GSVYAASLIIGFGFGAQVP+LFAIISELFGLK+YST+FNCGQLAVP GSYILN
Sbjct: 423 FIAFPYKGSVYAASLIIGFGFGAQVPMLFAIISELFGLKYYSTIFNCGQLAVPIGSYILN 482

Query: 484 VDVIGKLYDMEAIRQGGKKTGKGLTCTGANCFSNSFTILAAVTFCGALSSLVLAYRTRDF 543
           VDVIG+LYD+EA + GG K GKGLTCTG  CFS SF ILA V   GA++SLVLAYRTRDF
Sbjct: 483 VDVIGRLYDIEAAKLGGVKNGKGLTCTGTPCFSGSFLILAVVVLFGAVASLVLAYRTRDF 542

Query: 544 YKGETYKKYREDMWIPKADMEFYCLDNRK 563
           YKG+ Y +YREDMWIP++DMEFYC+D+RK
Sbjct: 543 YKGDVYNRYREDMWIPQSDMEFYCIDDRK 571

BLAST of MS006874 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 595.5 bits (1534), Expect = 4.4e-170
Identity = 316/569 (55.54%), Postives = 403/569 (70.83%), Query Frame = 0

Query: 13  FVKQVAEGRWFSVFASFLIMIGAGSTYLFGTYSKLLKTQFDYDQTQVNTLGFAKDLGSNF 72
           F+     GRWF VFASFLIM  AG+TYLFGTYSK +K+   YDQT +N LGF KDLG+N 
Sbjct: 9   FLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANV 68

Query: 73  GVFAGLLAEVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMCLYICLAANSQNF 132
           GV +GL+AEV P+W +  +GS++NF  YFMIWL+VT ++A P+ W MCLYIC+ ANSQNF
Sbjct: 69  GVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNF 128

Query: 133 ANTAVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHENPASVILLLAWFPS 192
           ANT  LVT VKNFPE RG++LGLLKG+VG+ GAI TQ+Y AIYGH++  S+ILL+AW P+
Sbjct: 129 ANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDS-KSLILLIAWLPA 188

Query: 193 VISFVFVLSIRTIKVRKHPEELKVFYHLLYVSIILALYILFLTLTQKQAVFSHAGYVGGA 252
            +S VFV  IR  KV +   EL VFY  LY+SI LAL+++ + + +KQ  FS A Y   A
Sbjct: 189 AVSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAASA 248

Query: 253 SVIVCLLCLPLLIAIREEFMLFNLNKQNQDGHVAVLIHDQKPPPKRALEED--------- 312
           ++   LL +PL +++++E  ++N+ K   +    V +  +KP  +  L++D         
Sbjct: 249 TICCALLFVPLTVSVKQELEVWNMMKLPIEEPSEVKV--EKPKKELDLDQDKAAKVNGEE 308

Query: 313 -----AIDSVSIKPERGDDFTIFQALLSKDMALLFIATLSGCGSSIAAIDNIGQIAESLQ 372
                   +V   P RG+D+TI QALLS DM +LF+AT  G GSS+ A+DN+GQI ESL 
Sbjct: 309 KETKSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESLG 368

Query: 373 YPPHSIAIFVSWVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLLTCIGQLLIAFP 432
           YP H+++ FVS VSI+N+FGRVFSGF+SE L+ KYKLPRPLM     LL+C G LLIAFP
Sbjct: 369 YPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIAFP 428

Query: 433 YRGSVYAASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPFGSYILNVDVIG 492
             GSVY AS+++GF FGAQ+PLLFAIISELFGLK+YSTLFNCGQLA P GSYILNV V G
Sbjct: 429 VPGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRVTG 488

Query: 493 KLYDMEAIRQ-----GGKKTGKGLTCTGANCFSNSFTILAAVTFCGALSSLVLAYRTRDF 552
            LYD EA++Q       +K  K LTC G+ C+   F ILAAVTF GAL SL LA RTR+F
Sbjct: 489 MLYDKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRTREF 548

Query: 553 YKGETYKKYREDMWIPKADMEFYCLDNRK 563
           YKG+ YKK+RE    P+++ E    D+RK
Sbjct: 549 YKGDIYKKFRES---PESESEL-VPDSRK 570

BLAST of MS006874 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 537.7 bits (1384), Expect = 1.1e-152
Identity = 284/561 (50.62%), Postives = 376/561 (67.02%), Query Frame = 0

Query: 16  QVAEGRWFSVFASFLIMIGAGSTYLFGTYSKLLKTQFDYDQTQVNTLGFAKDLGSNFGVF 75
           Q+  GRWF  F S LIM  AG+TY+FG YS  +K    YDQT +N L F KDLG+N GV 
Sbjct: 16  QILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVL 75

Query: 76  AGLLAEVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMCLYICLAANSQNFANT 135
           AGLL EV P W + L+G+ LNFF YFMIWL+VT RI+ PQ WHMCLYIC+ ANSQ+FANT
Sbjct: 76  AGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANT 135

Query: 136 AVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHENPASVILLLAWFPSVIS 195
             LVT VKNFPE RG++LG+LKG+VG+ GAI+TQ+Y A YG E+   +IL++ W P+++S
Sbjct: 136 GSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYG-EDTKELILMIGWLPAIVS 195

Query: 196 FVFVLSIRTIKVRKHPEELKVFYHLLYVSIILALYILFLTLTQKQAVFSHAGYVGGASVI 255
           F F+ +IR +KV++   ELKVFY+ LY+S+ LA +++ + +  K + F+ + + G A+V+
Sbjct: 196 FAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVV 255

Query: 256 VCLLCLPLLIAIREEFMLFNLNKQ---NQDGHVAVLIHDQKPPPKRALEEDAIDSVSI-- 315
           + LL LP+++ I EE  L+   KQ   N    + V+    K       ++D  +S  +  
Sbjct: 256 IVLLLLPIIVVILEEKKLWK-EKQVALNDPAPINVVTEKPKLDSSEFKDDDGEESKEVVE 315

Query: 316 -------------KPERGDDFTIFQALLSKDMALLFIATLSGCGSSIAAIDNIGQIAESL 375
                         PERGDD+TI QAL S DM +LF+AT+ G G ++ AIDN+GQI  SL
Sbjct: 316 KVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIGNSL 375

Query: 376 QYPPHSIAIFVSWVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLLTCIGQLLIAF 435
            YP  S++ FVS VSI+N++GRV SG +SE  ++KYK PRPLM     LL+C G LLIAF
Sbjct: 376 GYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIAF 435

Query: 436 PYRGSVYAASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPFGSYILNVDVI 495
              G +Y AS+IIGF FGAQ PLLFAIISE+FGLK+YSTL+N G +A P GSY+LNV V 
Sbjct: 436 NVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLNVRVA 495

Query: 496 GKLYDMEAIRQ-----GGKKTGKGLTCTGANCFSNSFTILAAVTFCGALSSLVLAYRTRD 554
           G LYD+EA +Q       +  G+ L C G +CF  SF I+AAVT  G L S+VL  RT+ 
Sbjct: 496 GYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVIRTKK 555

BLAST of MS006874 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 305.4 bits (781), Expect = 9.1e-83
Identity = 200/541 (36.97%), Postives = 303/541 (56.01%), Query Frame = 0

Query: 16  QVAEGRWFSVFASFLIMIGAGSTYLFGTYSKLLKTQFDYDQTQVNTLGFAKDLGSNFGVF 75
           ++   +W ++ AS  I   +G++Y FG YS +LK+   YDQ+ ++T+   KD+G+N GVF
Sbjct: 2   EILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVF 61

Query: 76  AGLLAEVAPS----------------WVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHM 135
           +GLL   A S                WV+  VG+   F  YF+IW SVT  I  P    M
Sbjct: 62  SGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLM 121

Query: 136 CLYICLAANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHEN 195
           CL++ LAA SQ F NTA +V++V+NF +  G  +G++KGF+G+ GAIL Q+Y  +   + 
Sbjct: 122 CLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGD- 181

Query: 196 PASVILLLAWFPSVISFVFVLSIRTIKVRKHPEELKVFYHLLYVSIILALYILFLTLTQK 255
           PAS ILLLA  P+V+S + V+ +  I      ++ K    L  VS+I+A Y++ + + + 
Sbjct: 182 PASFILLLAVTPTVLS-LLVMPLVRIYETSVADDKKHLNGLSAVSLIIAAYLMIIIILKN 241

Query: 256 QAVFSHAGYVGGASVIVCLLCLPLLIAIREEFMLFNLNKQNQDGHVAVLIHDQKP---PP 315
               S    +     ++ +L LPLLIA R +          +DG    + HD  P    P
Sbjct: 242 TFGLSSWANIVTLVCLLVMLALPLLIARRAQ----------RDGMEKTVPHDYSPLISSP 301

Query: 316 KRALEEDAIDSVSIKPERG--DDFTIFQALLSKDMALLFIATLSGCGSSIAAIDNIGQIA 375
           K     +       K E G  ++  + QA+      LLF+A + G GS ++ I+NI QI 
Sbjct: 302 KATTSGNQSSEGDSKVEAGLSENLNLLQAMKKLSFWLLFLAMICGMGSGLSTINNIRQIG 361

Query: 376 ESLQYPPHSIAIFVSWVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLLTCIGQLL 435
           ESL+Y    I   VS  SI+NF GR  +G+ S+ L+ K   PRPL+ A++     IG L+
Sbjct: 362 ESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSIGHLI 421

Query: 436 IAFPYRGSVYAASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPFGSYILNV 495
           IA  ++G++Y  S+I+G  +G+Q  L+  I SELFG++H  T+FN   +A P GSYI +V
Sbjct: 422 IASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVASPIGSYIFSV 481

Query: 496 DVIGKLYDMEAIRQGGKKTGKGLTCTGANCFSNSFTILAAVTFCGALSSLVLAYRTRDFY 536
            +IG +YD  A       +G+G TC G++CF  SF I+A+V F G L ++VL +RT+  Y
Sbjct: 482 RLIGYIYDKTA-------SGEGNTCYGSHCFRLSFIIMASVAFFGFLVAIVLFFRTKTLY 523

BLAST of MS006874 vs. TAIR 10
Match: AT5G50630.1 (Major facilitator superfamily protein )

HSP 1 Score: 289.3 bits (739), Expect = 6.8e-78
Identity = 181/539 (33.58%), Postives = 293/539 (54.36%), Query Frame = 0

Query: 12  HFVKQVAEGRWFSVFASFLIMIGAGSTYLF-GTYSKLLKTQFDYDQTQVNTLGFAKDLGS 71
           H ++ +   RW     +  +   AG  YLF G+ S  +KT   Y+Q Q+  LG AK+LG 
Sbjct: 7   HKLELLVNDRWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGD 66

Query: 72  NFGVFAGLLAEVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMCLYICLAANSQ 131
             G  +G L+EV+PSWV+ LVG++ N F Y ++WL VT ++ N   W + + I +  N +
Sbjct: 67  AIGFVSGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGE 126

Query: 132 NFANTAVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHENPASVILLLAWF 191
            + NTA LV+ + NFPE RG ++G+LKGF G+ GAILTQ+YL ++   + +SVIL++A  
Sbjct: 127 TYYNTASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYL-MFNPSHDSSVILMVALG 186

Query: 192 PSVISFVFVLSIRTI----KVRKHPEELKVFYHLLYVSIILALYILFLTLTQKQAVFSHA 251
           P V+    +  +R +    +     ++L+ F  +    ++LA+Y+L L + Q     +  
Sbjct: 187 PPVVVLALLFIVRPVERSCRTNLRSDDLR-FLAIYGFCVVLAVYLLGLLVLQSVFDMTQT 246

Query: 252 GYVGGASVIVCLLCLPLLIAIREEFMLFN--------LNKQNQDGHVA--VLIHDQKPPP 311
                 +++V  + +P+L+     F+  N            N D H A  ++    +PP 
Sbjct: 247 IITTSGAILVIFMVVPVLVPFSSVFISGNNVTSVKPEEGTSNVDQHEARTLIERSDRPPE 306

Query: 312 KRALEEDAIDSVSIKPERGDDFTIFQALLSKDMALLFIATLSGCGSSIAAIDNIGQIAES 371
           K+             P  G+DFT+ QAL   D  L+F++ + G GS I  IDN+GQI  S
Sbjct: 307 KKR-----------APCIGEDFTLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYS 366

Query: 372 LQYPPHSIAIFVSWVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLLTCIGQLLIA 431
           L Y   +  IFVS +SI NF GRV  G+ SE ++ K  LPR L  +    +  +G +  A
Sbjct: 367 LGY--SNTKIFVSLISISNFLGRVAGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYYA 426

Query: 432 FPYRGSVYAASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPFGSYILNVDV 491
             + G +Y  +++IG G+GA   +  A +S++FGLK + +L+N    A+P GS++ +  +
Sbjct: 427 IDWPGKIYVVTIVIGMGYGAHWAIAPASVSDIFGLKSFGSLYNFQITALPIGSFVFSGVI 486

Query: 492 IGKLYDMEAIRQGGKKT-GKGLTCTGANCFSNSFTILAAVTFCGALSSLVLAYRTRDFY 535
              +YD  A +Q G  T  + L CTG+ C+S + ++++ +     + SL + YRTR FY
Sbjct: 487 ASNIYDYYARKQAGPTTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFY 530

BLAST of MS006874 vs. TAIR 10
Match: AT5G50520.1 (Major facilitator superfamily protein )

HSP 1 Score: 289.3 bits (739), Expect = 6.8e-78
Identity = 181/539 (33.58%), Postives = 293/539 (54.36%), Query Frame = 0

Query: 12  HFVKQVAEGRWFSVFASFLIMIGAGSTYLF-GTYSKLLKTQFDYDQTQVNTLGFAKDLGS 71
           H ++ +   RW     +  +   AG  YLF G+ S  +KT   Y+Q Q+  LG AK+LG 
Sbjct: 7   HKLELLVNDRWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGD 66

Query: 72  NFGVFAGLLAEVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMCLYICLAANSQ 131
             G  +G L+EV+PSWV+ LVG++ N F Y ++WL VT ++ N   W + + I +  N +
Sbjct: 67  AIGFVSGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGE 126

Query: 132 NFANTAVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHENPASVILLLAWF 191
            + NTA LV+ + NFPE RG ++G+LKGF G+ GAILTQ+YL ++   + +SVIL++A  
Sbjct: 127 TYYNTASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYL-MFNPSHDSSVILMVALG 186

Query: 192 PSVISFVFVLSIRTI----KVRKHPEELKVFYHLLYVSIILALYILFLTLTQKQAVFSHA 251
           P V+    +  +R +    +     ++L+ F  +    ++LA+Y+L L + Q     +  
Sbjct: 187 PPVVVLALLFIVRPVERSCRTNLRSDDLR-FLAIYGFCVVLAVYLLGLLVLQSVFDMTQT 246

Query: 252 GYVGGASVIVCLLCLPLLIAIREEFMLFN--------LNKQNQDGHVA--VLIHDQKPPP 311
                 +++V  + +P+L+     F+  N            N D H A  ++    +PP 
Sbjct: 247 IITTSGAILVIFMVVPVLVPFSSVFISGNNVTSVKPEEGTSNVDQHEARTLIERSDRPPE 306

Query: 312 KRALEEDAIDSVSIKPERGDDFTIFQALLSKDMALLFIATLSGCGSSIAAIDNIGQIAES 371
           K+             P  G+DFT+ QAL   D  L+F++ + G GS I  IDN+GQI  S
Sbjct: 307 KKR-----------APCIGEDFTLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYS 366

Query: 372 LQYPPHSIAIFVSWVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLLTCIGQLLIA 431
           L Y   +  IFVS +SI NF GRV  G+ SE ++ K  LPR L  +    +  +G +  A
Sbjct: 367 LGY--SNTKIFVSLISISNFLGRVAGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYYA 426

Query: 432 FPYRGSVYAASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPFGSYILNVDV 491
             + G +Y  +++IG G+GA   +  A +S++FGLK + +L+N    A+P GS++ +  +
Sbjct: 427 IDWPGKIYVVTIVIGMGYGAHWAIAPASVSDIFGLKSFGSLYNFQITALPIGSFVFSGVI 486

Query: 492 IGKLYDMEAIRQGGKKT-GKGLTCTGANCFSNSFTILAAVTFCGALSSLVLAYRTRDFY 535
              +YD  A +Q G  T  + L CTG+ C+S + ++++ +     + SL + YRTR FY
Sbjct: 487 ASNIYDYYARKQAGPTTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFY 530

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152849.10.0e+0099.64uncharacterized protein LOC111020473 [Momordica charantia][more]
XP_022152845.12.5e-23974.60uncharacterized protein LOC111020469 [Momordica charantia][more]
XP_023521059.17.2e-23973.81uncharacterized protein LOC111784657 [Cucurbita pepo subsp. pepo][more]
KAG6583549.11.4e-23773.46Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022964772.11.8e-23773.46uncharacterized protein LOC111464772 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
F4I9E17.9e-3125.94Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A6J1DHB00.0e+0099.64uncharacterized protein LOC111020473 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
A0A6J1DF431.2e-23974.60uncharacterized protein LOC111020469 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
A0A6J1I3B98.6e-23873.11uncharacterized protein LOC111469246 OS=Cucurbita maxima OX=3661 GN=LOC111469246... [more]
A0A6J1HIM28.6e-23873.46uncharacterized protein LOC111464772 OS=Cucurbita moschata OX=3662 GN=LOC1114647... [more]
A0A6J1HYK51.1e-23772.58uncharacterized protein LOC111469251 OS=Cucurbita maxima OX=3661 GN=LOC111469251... [more]
Match NameE-valueIdentityDescription
AT2G28120.14.4e-17055.54Major facilitator superfamily protein [more]
AT2G39210.11.1e-15250.62Major facilitator superfamily protein [more]
AT1G74780.19.1e-8336.97Nodulin-like / Major Facilitator Superfamily protein [more]
AT5G50630.16.8e-7833.58Major facilitator superfamily protein [more]
AT5G50520.16.8e-7833.58Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 21..267
e-value: 7.4E-82
score: 274.6
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 15..216
e-value: 4.1E-6
score: 28.3
coord: 328..540
e-value: 1.8E-9
score: 39.3
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 23..532
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 6..545
NoneNo IPR availablePANTHERPTHR21576:SF29PROTEIN NUCLEAR FUSION DEFECTIVE 4coord: 6..545
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 20..520
e-value: 1.4542E-100
score: 307.641

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS006874.1MS006874.1mRNA