MS006870 (gene) Bitter gourd (TR) v1

Overview
NameMS006870
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionprotein NUCLEAR FUSION DEFECTIVE 4-like
Locationscaffold871: 281663 .. 283405 (+)
RNA-Seq ExpressionMS006870
SyntenyMS006870
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCAACGACGGAGCCGCCGAAGCATGGATTTTTATCAGACAAGTTGCGCGGGGACGGTGGTTCTCGGTGTTCGCGTCCTTCATGATCATGATCGGCGCCGGCTCAACTTACATGTTCGGGACTTACTCCAAAGCCATAAAGACCGAATTCAATTACAGCCAAACCGAAGTTAACACATTGGGATTCGCCAAGGACCTCGGCTCGAACGTCGGAGTTTTCGCCGGTCTTCTCGCCGAGGTGGCTCCGCTGTGGGTCCTCTTCATCGTCGGCTCAGCCCTCAACTTCTTCAGCTACTTCATGATTTGGCTCTCCGTCACCCGCCGCATTGCCAAGCCCGAATTCTGGCACATGTTCATCTACATCTGCTTGGCTACCAACTCACAGAATTTCGCCAACACCGCGGTTTTGGTCACCAGCGTCAGAAACTTCCCCGACCGAAGAGGGATCATTCTGGGGCTTCTCAAGGGCTTCGTTGGCATCGGAGGAGCCATCTTGACCCAGTTGTATTTAGCCGTGTACGGCCATGAAGACCCATCGAATCTTATTCTCCTTCTTTCGTGGTTCCCCTCATTCGTATCTCTTGTGCTTTTGCTCGCAATTCGACCAATCAAAATCCGCAAACATCCCGATGAGCTGAAAGTGCTTTACCACTTGCTTTACGTCTCCATACTTCTGGCTTTGTTTATCTTGTTCTTGACCTTAATGCAAAAACAAGTGGTTTTCTCTCACACTGGATATGCGAGTGGCGCTTTCGCCATTTTGGCCTTGTTGGCTCTCCCCCTTTTGATCACTGTTAGAGAAGAGCTCATGCTCTACAAACTCAACAAACAAAACAATGACCCATCCGTCATCGTTTCCATCGTTGGCGAGCAGAAGCTCCCGATGGTAACCGTGCCCCAGAACACGCCGCCGACGTCCACGCTGCAGGAAATGACCGAAATCTCGCCGTCCTGCCTCGAAAACATATGCAACAAGCCTGAAAGAGGGGAGGATTTCACCATCTTGCAAGCCATCTTCAGCGTAGACATGGCTCTTGTCTATCTCGCCACCTTTACTGGCTGTGGCTCCTCCCTTGCTTCCATTGACAACATAGGCCAAATTGGTGAGTCTCTTCACTACCCAACTCAATCCATAGCCATTTTCGTCTCTTGGGTTTCCGTTTTCAATTTCTTCGGCCGCGTCTTCTCCGGCTTCATCTCCGAAACCCTCATGACCAAATACAAATTACCTCGCCCTCTCATGTTTGCTTTTTCCTTTCTCCTCACTTGCATCGGCCAGCTCTTCATCGCCTTCCCATCTCCTGGCTCGGTCTACGTCGCTTCCTTGATCATTGGGTTCGGGTTCGGAGCCCAAGTTCCCCTGTTGTTCGCCATCATCTCCGAGCTCTTTGGTCTCAAACGCTACTCAACGCTCTTTAACTGTGGACAACTGGTGGTCCCGTTTGGATCCTATATACTAAACGTGGATATAGTGGGGAAGTTGTACGATATTGAAGCCATAAAAGAAGGTAGTAAGAAGACTGGCAAGGGGCTAACTTGCACCGGGGCTCATTGCTTCGGCTGGTCTTTCACAATTTTGGCATCAGTGACATTGTTTGGAGCTCTGGTTGCGCTTGTGCTGGCTTACAGGACTCGAGAGTTTTACAAAGGCGACGTATATAAGAAATACAGAGAGGACATGTGGATTCCACAATCGGAAATGGAATTCTATAGGTTAGATAACAAGAAGAACATTGATGAT

mRNA sequence

ATGGCCAACGACGGAGCCGCCGAAGCATGGATTTTTATCAGACAAGTTGCGCGGGGACGGTGGTTCTCGGTGTTCGCGTCCTTCATGATCATGATCGGCGCCGGCTCAACTTACATGTTCGGGACTTACTCCAAAGCCATAAAGACCGAATTCAATTACAGCCAAACCGAAGTTAACACATTGGGATTCGCCAAGGACCTCGGCTCGAACGTCGGAGTTTTCGCCGGTCTTCTCGCCGAGGTGGCTCCGCTGTGGGTCCTCTTCATCGTCGGCTCAGCCCTCAACTTCTTCAGCTACTTCATGATTTGGCTCTCCGTCACCCGCCGCATTGCCAAGCCCGAATTCTGGCACATGTTCATCTACATCTGCTTGGCTACCAACTCACAGAATTTCGCCAACACCGCGGTTTTGGTCACCAGCGTCAGAAACTTCCCCGACCGAAGAGGGATCATTCTGGGGCTTCTCAAGGGCTTCGTTGGCATCGGAGGAGCCATCTTGACCCAGTTGTATTTAGCCGTGTACGGCCATGAAGACCCATCGAATCTTATTCTCCTTCTTTCGTGGTTCCCCTCATTCGTATCTCTTGTGCTTTTGCTCGCAATTCGACCAATCAAAATCCGCAAACATCCCGATGAGCTGAAAGTGCTTTACCACTTGCTTTACGTCTCCATACTTCTGGCTTTGTTTATCTTGTTCTTGACCTTAATGCAAAAACAAGTGGTTTTCTCTCACACTGGATATGCGAGTGGCGCTTTCGCCATTTTGGCCTTGTTGGCTCTCCCCCTTTTGATCACTGTTAGAGAAGAGCTCATGCTCTACAAACTCAACAAACAAAACAATGACCCATCCGTCATCGTTTCCATCGTTGGCGAGCAGAAGCTCCCGATGGTAACCGTGCCCCAGAACACGCCGCCGACGTCCACGCTGCAGGAAATGACCGAAATCTCGCCGTCCTGCCTCGAAAACATATGCAACAAGCCTGAAAGAGGGGAGGATTTCACCATCTTGCAAGCCATCTTCAGCGTAGACATGGCTCTTGTCTATCTCGCCACCTTTACTGGCTGTGGCTCCTCCCTTGCTTCCATTGACAACATAGGCCAAATTGGTGAGTCTCTTCACTACCCAACTCAATCCATAGCCATTTTCGTCTCTTGGGTTTCCGTTTTCAATTTCTTCGGCCGCGTCTTCTCCGGCTTCATCTCCGAAACCCTCATGACCAAATACAAATTACCTCGCCCTCTCATGTTTGCTTTTTCCTTTCTCCTCACTTGCATCGGCCAGCTCTTCATCGCCTTCCCATCTCCTGGCTCGGTCTACGTCGCTTCCTTGATCATTGGGTTCGGGTTCGGAGCCCAAGTTCCCCTGTTGTTCGCCATCATCTCCGAGCTCTTTGGTCTCAAACGCTACTCAACGCTCTTTAACTGTGGACAACTGGTGGTCCCGTTTGGATCCTATATACTAAACGTGGATATAGTGGGGAAGTTGTACGATATTGAAGCCATAAAAGAAGGTAGTAAGAAGACTGGCAAGGGGCTAACTTGCACCGGGGCTCATTGCTTCGGCTGGTCTTTCACAATTTTGGCATCAGTGACATTGTTTGGAGCTCTGGTTGCGCTTGTGCTGGCTTACAGGACTCGAGAGTTTTACAAAGGCGACGTATATAAGAAATACAGAGAGGACATGTGGATTCCACAATCGGAAATGGAATTCTATAGGTTAGATAACAAGAAGAACATTGATGAT

Coding sequence (CDS)

ATGGCCAACGACGGAGCCGCCGAAGCATGGATTTTTATCAGACAAGTTGCGCGGGGACGGTGGTTCTCGGTGTTCGCGTCCTTCATGATCATGATCGGCGCCGGCTCAACTTACATGTTCGGGACTTACTCCAAAGCCATAAAGACCGAATTCAATTACAGCCAAACCGAAGTTAACACATTGGGATTCGCCAAGGACCTCGGCTCGAACGTCGGAGTTTTCGCCGGTCTTCTCGCCGAGGTGGCTCCGCTGTGGGTCCTCTTCATCGTCGGCTCAGCCCTCAACTTCTTCAGCTACTTCATGATTTGGCTCTCCGTCACCCGCCGCATTGCCAAGCCCGAATTCTGGCACATGTTCATCTACATCTGCTTGGCTACCAACTCACAGAATTTCGCCAACACCGCGGTTTTGGTCACCAGCGTCAGAAACTTCCCCGACCGAAGAGGGATCATTCTGGGGCTTCTCAAGGGCTTCGTTGGCATCGGAGGAGCCATCTTGACCCAGTTGTATTTAGCCGTGTACGGCCATGAAGACCCATCGAATCTTATTCTCCTTCTTTCGTGGTTCCCCTCATTCGTATCTCTTGTGCTTTTGCTCGCAATTCGACCAATCAAAATCCGCAAACATCCCGATGAGCTGAAAGTGCTTTACCACTTGCTTTACGTCTCCATACTTCTGGCTTTGTTTATCTTGTTCTTGACCTTAATGCAAAAACAAGTGGTTTTCTCTCACACTGGATATGCGAGTGGCGCTTTCGCCATTTTGGCCTTGTTGGCTCTCCCCCTTTTGATCACTGTTAGAGAAGAGCTCATGCTCTACAAACTCAACAAACAAAACAATGACCCATCCGTCATCGTTTCCATCGTTGGCGAGCAGAAGCTCCCGATGGTAACCGTGCCCCAGAACACGCCGCCGACGTCCACGCTGCAGGAAATGACCGAAATCTCGCCGTCCTGCCTCGAAAACATATGCAACAAGCCTGAAAGAGGGGAGGATTTCACCATCTTGCAAGCCATCTTCAGCGTAGACATGGCTCTTGTCTATCTCGCCACCTTTACTGGCTGTGGCTCCTCCCTTGCTTCCATTGACAACATAGGCCAAATTGGTGAGTCTCTTCACTACCCAACTCAATCCATAGCCATTTTCGTCTCTTGGGTTTCCGTTTTCAATTTCTTCGGCCGCGTCTTCTCCGGCTTCATCTCCGAAACCCTCATGACCAAATACAAATTACCTCGCCCTCTCATGTTTGCTTTTTCCTTTCTCCTCACTTGCATCGGCCAGCTCTTCATCGCCTTCCCATCTCCTGGCTCGGTCTACGTCGCTTCCTTGATCATTGGGTTCGGGTTCGGAGCCCAAGTTCCCCTGTTGTTCGCCATCATCTCCGAGCTCTTTGGTCTCAAACGCTACTCAACGCTCTTTAACTGTGGACAACTGGTGGTCCCGTTTGGATCCTATATACTAAACGTGGATATAGTGGGGAAGTTGTACGATATTGAAGCCATAAAAGAAGGTAGTAAGAAGACTGGCAAGGGGCTAACTTGCACCGGGGCTCATTGCTTCGGCTGGTCTTTCACAATTTTGGCATCAGTGACATTGTTTGGAGCTCTGGTTGCGCTTGTGCTGGCTTACAGGACTCGAGAGTTTTACAAAGGCGACGTATATAAGAAATACAGAGAGGACATGTGGATTCCACAATCGGAAATGGAATTCTATAGGTTAGATAACAAGAAGAACATTGATGAT

Protein sequence

MANDGAAEAWIFIRQVARGRWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNTLGFAKDLGSNVGVFAGLLAEVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFIYICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPSNLILLLSWFPSFVSLVLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQKQVVFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMVTVPQNTPPTSTLQEMTEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGSSLASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFAFSFLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQLVVPFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALVALVLAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNIDD
Homology
BLAST of MS006870 vs. NCBI nr
Match: XP_022152846.1 (uncharacterized protein LOC111020470 [Momordica charantia])

HSP 1 Score: 896.7 bits (2316), Expect = 1.0e-256
Identity = 461/464 (99.35%), Postives = 464/464 (100.00%), Query Frame = 0

Query: 118 MFIYICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHE 177
           MFIYICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHE
Sbjct: 1   MFIYICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHE 60

Query: 178 DPSNLILLLSWFPSFVSLVLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQ 237
           DPSNLILLLSWFPSF+SL+LLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQ
Sbjct: 61  DPSNLILLLSWFPSFISLLLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQ 120

Query: 238 KQVVFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMV 297
           KQVVFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMV
Sbjct: 121 KQVVFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMV 180

Query: 298 TVPQNTPPTSTLQEMTEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGS 357
           TVPQNTPPTSTLQE+TEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGS
Sbjct: 181 TVPQNTPPTSTLQEITEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGS 240

Query: 358 SLASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFA 417
           SLASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFA
Sbjct: 241 SLASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFA 300

Query: 418 FSFLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQ 477
           FSFLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQ
Sbjct: 301 FSFLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQ 360

Query: 478 LVVPFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALV 537
           LVVPFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALV
Sbjct: 361 LVVPFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALV 420

Query: 538 ALVLAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNIDD 582
           ALVLAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNIDD
Sbjct: 421 ALVLAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNIDD 464

BLAST of MS006870 vs. NCBI nr
Match: XP_022152849.1 (uncharacterized protein LOC111020473 [Momordica charantia])

HSP 1 Score: 852.0 bits (2200), Expect = 2.9e-243
Identity = 430/577 (74.52%), Postives = 495/577 (85.79%), Query Frame = 0

Query: 3   NDGAAEAWIFIRQVARGRWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNTLG 62
           N+ AA +W F++QVA GRWFSVFASF+IMIGAGSTY+FGTYSK +KT+F+Y QT+VNTLG
Sbjct: 4   NNHAAASWHFVKQVAEGRWFSVFASFLIMIGAGSTYLFGTYSKLLKTQFDYDQTQVNTLG 63

Query: 63  FAKDLGSNVGVFAGLLAEVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFIYI 122
           FAKDLGSN GVFAGLLAEVAP WVLF+VGS+LNFFSYFMIWLSVTRRIA P+FWHM +YI
Sbjct: 64  FAKDLGSNFGVFAGLLAEVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMCLYI 123

Query: 123 CLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPSNL 182
           CLA NSQNFANTAVLVTSV+NFP+RRGIILGLLKGFVGIGGAILTQ+YLA+YGHE+ +++
Sbjct: 124 CLAANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHENQASV 183

Query: 183 ILLLSWFPSFVSLVLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQKQVVF 242
           ILLL+WFPS +S V +L+IR IK+RKHP+ELKV YHLLYVSI+LAL+ILFLTL QKQ VF
Sbjct: 184 ILLLAWFPSVISFVFVLSIRTIKVRKHPEELKVFYHLLYVSIILALYILFLTLTQKQAVF 243

Query: 243 SHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMVTVPQN 302
           SH GY  GA  I+ LL LPLLI +REE ML+ LNKQN D  V V ++ +QK         
Sbjct: 244 SHAGYVGGASVIVCLLCLPLLIAIREEFMLFNLNKQNQDGHVAV-LIHDQK--------- 303

Query: 303 TPPTSTLQEMTEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGSSLASI 362
            PP   L+E        ++++  KPERG+DFTI QA+ S DMAL+++AT +GCGSS+A+I
Sbjct: 304 PPPKRALEE------DAIDSVSIKPERGDDFTIFQALLSKDMALLFIATLSGCGSSIAAI 363

Query: 363 DNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFAFSFLL 422
           DNIGQI ESL YP  SIAIFVSWVS+FNFFGRVFSGFISETLM KYKLPRPLMFA SFLL
Sbjct: 364 DNIGQIAESLQYPPHSIAIFVSWVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLL 423

Query: 423 TCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQLVVPF 482
           TCIGQL IAFP  GSVY ASLIIGFGFGAQVPLLFAIISELFGLK YSTLFNCGQL VPF
Sbjct: 424 TCIGQLLIAFPYRGSVYAASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPF 483

Query: 483 GSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALVALVLA 542
           GSYILNVD++GKLYD+EAI++G KKTGKGLTCTGA+CF  SFTILA+VT FGAL +LVLA
Sbjct: 484 GSYILNVDVIGKLYDMEAIRQGGKKTGKGLTCTGANCFSNSFTILAAVTFFGALSSLVLA 543

Query: 543 YRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNI 580
           YRTR+FYKG+ YKKYREDMWIP+++MEFY LDN+K +
Sbjct: 544 YRTRDFYKGETYKKYREDMWIPKADMEFYCLDNRKAV 564

BLAST of MS006870 vs. NCBI nr
Match: XP_022152845.1 (uncharacterized protein LOC111020469 [Momordica charantia])

HSP 1 Score: 843.6 bits (2178), Expect = 1.0e-240
Identity = 417/581 (71.77%), Postives = 497/581 (85.54%), Query Frame = 0

Query: 1   MANDGAAEAWIFIRQVARGRWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNT 60
           MA + A ++  F+RQV  GRWFSVFASF+IMIGAGSTY+FGTYSK +K +F+YSQT+VNT
Sbjct: 1   MAGNRAEQSLYFVRQVVEGRWFSVFASFLIMIGAGSTYVFGTYSKVMKAQFDYSQTQVNT 60

Query: 61  LGFAKDLGSNVGVFAGLLAEVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFI 120
           LGF KDLGSN+GVFAGLLAEVAP WVLF+VGS+LNF SYFMIWLS+T RIAKPEFWHM  
Sbjct: 61  LGFFKDLGSNLGVFAGLLAEVAPPWVLFVVGSSLNFVSYFMIWLSLTHRIAKPEFWHMCT 120

Query: 121 YICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPS 180
           YICLA NSQNFANTAVLVTSV+NFP+RRGIILGLLKGFVG+GGAILTQ+YLA+YGHE+  
Sbjct: 121 YICLAANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVGVGGAILTQMYLAIYGHENQV 180

Query: 181 NLILLLSWFPSFVSLVLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQKQV 240
           NL+LLLSWFP+ +SLV LL IRPIK+RKHPDEL+V+YHLLYVSI+LALF+LFLT+ QKQ 
Sbjct: 181 NLVLLLSWFPTIISLVFLLTIRPIKVRKHPDELRVMYHLLYVSIILALFLLFLTITQKQA 240

Query: 241 VFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMVTVP 300
           VFS  GY SGA  I +LL++PLLI VREE +L+KLNK+  +PSV VSI+ +Q+       
Sbjct: 241 VFSQEGYMSGAAVIASLLSIPLLIAVREEFLLFKLNKETTNPSVTVSILDQQR------- 300

Query: 301 QNTPPTSTLQEMTEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGSSLA 360
               P   L E+ E  PSC+ ++ NKP+RG+D+TILQA+FS DMAL+++    GCGSS+A
Sbjct: 301 ----PAPMLDEIVEAEPSCMSSVFNKPQRGDDYTILQALFSKDMALIFVGALCGCGSSIA 360

Query: 361 SIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFAFSF 420
           +IDN+GQIGESL+YP QSI IF+S +S+FNFFGRV SGFISETLMTK+KLPRPLMFA + 
Sbjct: 361 AIDNLGQIGESLYYPAQSITIFISLISIFNFFGRVCSGFISETLMTKHKLPRPLMFALAH 420

Query: 421 LLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQLVV 480
           LLTCIG LF+AFP  GS+Y+AS+++GFGFGAQVP++FAIISELFGLKRY+TLFN GQL V
Sbjct: 421 LLTCIGLLFVAFPFHGSIYIASVVVGFGFGAQVPMVFAIISELFGLKRYATLFNFGQLSV 480

Query: 481 PFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALVALV 540
           P GSYILNVDI+GKLYD+EA K+G+ K GKGLTCTG  CF  SFTILA+VTLFGAL +LV
Sbjct: 481 PIGSYILNVDIIGKLYDMEARKQGTTKNGKGLTCTGHECFSTSFTILATVTLFGALTSLV 540

Query: 541 LAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNIDD 582
           LA+RTR+FYKGDVY KYREDMW+P+S+MEFY LDNKK  +D
Sbjct: 541 LAFRTRDFYKGDVYNKYREDMWMPESDMEFYCLDNKKKSND 570

BLAST of MS006870 vs. NCBI nr
Match: XP_022970365.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima] >XP_022970399.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 842.8 bits (2176), Expect = 1.8e-240
Identity = 416/581 (71.60%), Postives = 494/581 (85.03%), Query Frame = 0

Query: 1   MANDGAAEAWIFIRQVARGRWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNT 60
           M N G +EAW FI+QV  GRWFSVFA  ++MIG G+TY+F TYSK IKT+F+YSQT++NT
Sbjct: 1   MGNKGGSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNVGVFAGLLAEVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFI 120
           LGFAKDLGSN+G+ AGLLAEV P WVLFI+G+  NF  +F+IWLS+T RIAKP+FW MFI
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPS 180
            +C  TNS N+ANTA++VTSVRNFPDRRGIILGLLKG+VGIGGAI++Q YLA+YGH DPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPS 180

Query: 181 NLILLLSWFPSFVSLVLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQKQV 240
           NL+LL +W PS + L+L  +IRPI+IRKHP+ELKV Y LLYVSILLA+FILFLT+ QKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 VFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMVTVP 300
           VFS  GY  GA  +++LL +P+LI  REEL+LYKLNKQ   PSV VS+V  Q  P     
Sbjct: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMV-HQNPP----- 300

Query: 301 QNTPPTSTLQEMTEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGSSLA 360
             T P S   E+ EISPSC ENI NKPERGEDFTILQA+FS DMAL+ LATF+GCGSSLA
Sbjct: 301 --TLPASADSEIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLA 360

Query: 361 SIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFAFSF 420
           +IDN+GQ+GESL YP ++I I VSWVS+FNFFGRVFSGFISETLMTKYKLPRPL F+F+F
Sbjct: 361 AIDNLGQVGESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAF 420

Query: 421 LLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQLVV 480
           L+TC+GQLFIA+P PGSVY+A++IIGFGFGAQ P+LFA+ISE+FGL+RYSTLFNCGQL V
Sbjct: 421 LITCVGQLFIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAV 480

Query: 481 PFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALVALV 540
           P GSYILNVD+VGKLYD+EA++EG K  GKGLTCTGAHCFG SFTILA+ TLFGA+V LV
Sbjct: 481 PLGSYILNVDVVGKLYDMEALREGKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLV 540

Query: 541 LAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNIDD 582
           LAYRTREFYK DVYK + E++WIPQ++MEFYRLD+KK+I+D
Sbjct: 541 LAYRTREFYKRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED 573

BLAST of MS006870 vs. NCBI nr
Match: XP_022964774.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 842.0 bits (2174), Expect = 3.0e-240
Identity = 416/581 (71.60%), Postives = 492/581 (84.68%), Query Frame = 0

Query: 1   MANDGAAEAWIFIRQVARGRWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNT 60
           M N G +EAW FI+QV  GRWFSVFA  ++MIG G+TY+F TYSK IKT+F+YSQT++NT
Sbjct: 1   MGNKGGSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNVGVFAGLLAEVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFI 120
           LGFAKDLGSN+G+ AGLLAEV P WVLFI+G+  NF  +F+IWLS+T RIAKP+FW MFI
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPS 180
            +C ATNS N+ANTA++VTSVRNFPDRRGIILGLLKG+VGIGGAI++QLYLA+YGH DPS
Sbjct: 121 LVCFATNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPS 180

Query: 181 NLILLLSWFPSFVSLVLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQKQV 240
           NL+LL +W PS + L+L  +IRPI+IRKHPDELKV Y LLYVSILLA+FILFLT+ QKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 VFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMVTVP 300
           VFS  GY  GA  ++ LL +P+LI  REEL+LYKLNKQ   PSV +S++  Q  P     
Sbjct: 241 VFSQAGYVGGASFVVCLLCVPILIACREELLLYKLNKQTAAPSVALSML-HQNPP----- 300

Query: 301 QNTPPTSTLQEMTEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGSSLA 360
             T PTS   E+ EISPSC  NI NKPERGEDFTILQA+FS DMAL+ LATF+GCGSSLA
Sbjct: 301 --TLPTSADSEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLA 360

Query: 361 SIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFAFSF 420
           +IDN+GQ+GESL Y  ++I I VSWVS+FNFFGRVFSGFISETLMTKYKLPRPL FAF+F
Sbjct: 361 AIDNLGQVGESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAF 420

Query: 421 LLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQLVV 480
           L+TC+GQLFIA+P PGS+Y+A++IIGFGFGAQ P+LFA+ISE+FGLKRYSTLFNCGQL  
Sbjct: 421 LITCVGQLFIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAA 480

Query: 481 PFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALVALV 540
           PFGSYILNVD+VGKLYD+EA++E  +  GKGLTCTGAHCFG SFT+LA+ TLFGA+V  V
Sbjct: 481 PFGSYILNVDVVGKLYDMEALRERKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFV 540

Query: 541 LAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNIDD 582
           LAYRTREFYK DVYK + E++WIPQ+EMEFYRLDNKKNI+D
Sbjct: 541 LAYRTREFYKRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

BLAST of MS006870 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 5.0e-28
Identity = 138/539 (25.60%), Postives = 231/539 (42.86%), Query Frame = 0

Query: 20  RWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNTLGFAKDLGSNVGVFAGLLA 79
           +W  + A+  I    G+ + F  YS  +K+    SQ  +N L  A DLG   G  +G+  
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 80  EVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFIYIC--LATNSQNFANTAVL 139
              PL V+    +A+ F  Y + WL +T  I  P   +  +++C  LA  S  + NTA  
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP---YSLVFLCCLLAGLSICWFNTACF 162

Query: 140 VTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPSNLILLL-SWFP---SFV 199
           +  +R+FP+ R + L L   F GI  A+ +  + A+  +   SNL LLL S  P   SF 
Sbjct: 163 ILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAI--NPSSSNLYLLLNSLVPLVVSFA 222

Query: 200 SLVLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQKQVVFSHTGYASGAFA 259
           +L  +L    +      D  +   H+  +  +LA+   F  L+      S      GA  
Sbjct: 223 ALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIGAVV 282

Query: 260 ILAL-LALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMVTVPQNTPPTSTLQEM 319
           +L   L  PLL+  R+  +     + N++ S  V         M+ + +     +++   
Sbjct: 283 LLVFPLCAPLLVYARDYFLPVINARLNHESSGYV---------MLNIDELKNQKTSVSSK 342

Query: 320 TEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGSSLASIDNIGQIGESL 379
           T           N    G++ +    I  ++  L Y+A F G    L   +N+GQI +SL
Sbjct: 343 TGYEHMGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSL 402

Query: 380 HYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFAFSFLLTCIGQLFIAF 439
               Q+    V+  S F+FFGR+ S    + +  +++L R   FA + L T I    +A 
Sbjct: 403 ---GQNSTTLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAV 462

Query: 440 PS--PGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQLVVPFGSYILNVD 499
            S    ++  A+ +IG   G       +I S+LFG        N     +P GS +    
Sbjct: 463 SSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGY- 522

Query: 500 IVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALVALVLAYRTREFY 550
           I   +Y+  A  + +      + C G  C+  +F     +++ G + +L L  RT+  Y
Sbjct: 523 IAASIYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of MS006870 vs. ExPASy TrEMBL
Match: A0A6J1DIZ3 (uncharacterized protein LOC111020470 OS=Momordica charantia OX=3673 GN=LOC111020470 PE=4 SV=1)

HSP 1 Score: 896.7 bits (2316), Expect = 5.0e-257
Identity = 461/464 (99.35%), Postives = 464/464 (100.00%), Query Frame = 0

Query: 118 MFIYICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHE 177
           MFIYICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHE
Sbjct: 1   MFIYICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHE 60

Query: 178 DPSNLILLLSWFPSFVSLVLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQ 237
           DPSNLILLLSWFPSF+SL+LLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQ
Sbjct: 61  DPSNLILLLSWFPSFISLLLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQ 120

Query: 238 KQVVFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMV 297
           KQVVFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMV
Sbjct: 121 KQVVFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMV 180

Query: 298 TVPQNTPPTSTLQEMTEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGS 357
           TVPQNTPPTSTLQE+TEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGS
Sbjct: 181 TVPQNTPPTSTLQEITEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGS 240

Query: 358 SLASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFA 417
           SLASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFA
Sbjct: 241 SLASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFA 300

Query: 418 FSFLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQ 477
           FSFLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQ
Sbjct: 301 FSFLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQ 360

Query: 478 LVVPFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALV 537
           LVVPFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALV
Sbjct: 361 LVVPFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALV 420

Query: 538 ALVLAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNIDD 582
           ALVLAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNIDD
Sbjct: 421 ALVLAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNIDD 464

BLAST of MS006870 vs. ExPASy TrEMBL
Match: A0A6J1DHB0 (uncharacterized protein LOC111020473 OS=Momordica charantia OX=3673 GN=LOC111020473 PE=4 SV=1)

HSP 1 Score: 852.0 bits (2200), Expect = 1.4e-243
Identity = 430/577 (74.52%), Postives = 495/577 (85.79%), Query Frame = 0

Query: 3   NDGAAEAWIFIRQVARGRWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNTLG 62
           N+ AA +W F++QVA GRWFSVFASF+IMIGAGSTY+FGTYSK +KT+F+Y QT+VNTLG
Sbjct: 4   NNHAAASWHFVKQVAEGRWFSVFASFLIMIGAGSTYLFGTYSKLLKTQFDYDQTQVNTLG 63

Query: 63  FAKDLGSNVGVFAGLLAEVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFIYI 122
           FAKDLGSN GVFAGLLAEVAP WVLF+VGS+LNFFSYFMIWLSVTRRIA P+FWHM +YI
Sbjct: 64  FAKDLGSNFGVFAGLLAEVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMCLYI 123

Query: 123 CLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPSNL 182
           CLA NSQNFANTAVLVTSV+NFP+RRGIILGLLKGFVGIGGAILTQ+YLA+YGHE+ +++
Sbjct: 124 CLAANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHENQASV 183

Query: 183 ILLLSWFPSFVSLVLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQKQVVF 242
           ILLL+WFPS +S V +L+IR IK+RKHP+ELKV YHLLYVSI+LAL+ILFLTL QKQ VF
Sbjct: 184 ILLLAWFPSVISFVFVLSIRTIKVRKHPEELKVFYHLLYVSIILALYILFLTLTQKQAVF 243

Query: 243 SHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMVTVPQN 302
           SH GY  GA  I+ LL LPLLI +REE ML+ LNKQN D  V V ++ +QK         
Sbjct: 244 SHAGYVGGASVIVCLLCLPLLIAIREEFMLFNLNKQNQDGHVAV-LIHDQK--------- 303

Query: 303 TPPTSTLQEMTEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGSSLASI 362
            PP   L+E        ++++  KPERG+DFTI QA+ S DMAL+++AT +GCGSS+A+I
Sbjct: 304 PPPKRALEE------DAIDSVSIKPERGDDFTIFQALLSKDMALLFIATLSGCGSSIAAI 363

Query: 363 DNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFAFSFLL 422
           DNIGQI ESL YP  SIAIFVSWVS+FNFFGRVFSGFISETLM KYKLPRPLMFA SFLL
Sbjct: 364 DNIGQIAESLQYPPHSIAIFVSWVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLL 423

Query: 423 TCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQLVVPF 482
           TCIGQL IAFP  GSVY ASLIIGFGFGAQVPLLFAIISELFGLK YSTLFNCGQL VPF
Sbjct: 424 TCIGQLLIAFPYRGSVYAASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPF 483

Query: 483 GSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALVALVLA 542
           GSYILNVD++GKLYD+EAI++G KKTGKGLTCTGA+CF  SFTILA+VT FGAL +LVLA
Sbjct: 484 GSYILNVDVIGKLYDMEAIRQGGKKTGKGLTCTGANCFSNSFTILAAVTFFGALSSLVLA 543

Query: 543 YRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNI 580
           YRTR+FYKG+ YKKYREDMWIP+++MEFY LDN+K +
Sbjct: 544 YRTRDFYKGETYKKYREDMWIPKADMEFYCLDNRKAV 564

BLAST of MS006870 vs. ExPASy TrEMBL
Match: A0A6J1DF43 (uncharacterized protein LOC111020469 OS=Momordica charantia OX=3673 GN=LOC111020469 PE=4 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 5.0e-241
Identity = 417/581 (71.77%), Postives = 497/581 (85.54%), Query Frame = 0

Query: 1   MANDGAAEAWIFIRQVARGRWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNT 60
           MA + A ++  F+RQV  GRWFSVFASF+IMIGAGSTY+FGTYSK +K +F+YSQT+VNT
Sbjct: 1   MAGNRAEQSLYFVRQVVEGRWFSVFASFLIMIGAGSTYVFGTYSKVMKAQFDYSQTQVNT 60

Query: 61  LGFAKDLGSNVGVFAGLLAEVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFI 120
           LGF KDLGSN+GVFAGLLAEVAP WVLF+VGS+LNF SYFMIWLS+T RIAKPEFWHM  
Sbjct: 61  LGFFKDLGSNLGVFAGLLAEVAPPWVLFVVGSSLNFVSYFMIWLSLTHRIAKPEFWHMCT 120

Query: 121 YICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPS 180
           YICLA NSQNFANTAVLVTSV+NFP+RRGIILGLLKGFVG+GGAILTQ+YLA+YGHE+  
Sbjct: 121 YICLAANSQNFANTAVLVTSVKNFPERRGIILGLLKGFVGVGGAILTQMYLAIYGHENQV 180

Query: 181 NLILLLSWFPSFVSLVLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQKQV 240
           NL+LLLSWFP+ +SLV LL IRPIK+RKHPDEL+V+YHLLYVSI+LALF+LFLT+ QKQ 
Sbjct: 181 NLVLLLSWFPTIISLVFLLTIRPIKVRKHPDELRVMYHLLYVSIILALFLLFLTITQKQA 240

Query: 241 VFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMVTVP 300
           VFS  GY SGA  I +LL++PLLI VREE +L+KLNK+  +PSV VSI+ +Q+       
Sbjct: 241 VFSQEGYMSGAAVIASLLSIPLLIAVREEFLLFKLNKETTNPSVTVSILDQQR------- 300

Query: 301 QNTPPTSTLQEMTEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGSSLA 360
               P   L E+ E  PSC+ ++ NKP+RG+D+TILQA+FS DMAL+++    GCGSS+A
Sbjct: 301 ----PAPMLDEIVEAEPSCMSSVFNKPQRGDDYTILQALFSKDMALIFVGALCGCGSSIA 360

Query: 361 SIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFAFSF 420
           +IDN+GQIGESL+YP QSI IF+S +S+FNFFGRV SGFISETLMTK+KLPRPLMFA + 
Sbjct: 361 AIDNLGQIGESLYYPAQSITIFISLISIFNFFGRVCSGFISETLMTKHKLPRPLMFALAH 420

Query: 421 LLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQLVV 480
           LLTCIG LF+AFP  GS+Y+AS+++GFGFGAQVP++FAIISELFGLKRY+TLFN GQL V
Sbjct: 421 LLTCIGLLFVAFPFHGSIYIASVVVGFGFGAQVPMVFAIISELFGLKRYATLFNFGQLSV 480

Query: 481 PFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALVALV 540
           P GSYILNVDI+GKLYD+EA K+G+ K GKGLTCTG  CF  SFTILA+VTLFGAL +LV
Sbjct: 481 PIGSYILNVDIIGKLYDMEARKQGTTKNGKGLTCTGHECFSTSFTILATVTLFGALTSLV 540

Query: 541 LAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNIDD 582
           LA+RTR+FYKGDVY KYREDMW+P+S+MEFY LDNKK  +D
Sbjct: 541 LAFRTRDFYKGDVYNKYREDMWMPESDMEFYCLDNKKKSND 570

BLAST of MS006870 vs. ExPASy TrEMBL
Match: A0A6J1I5D7 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469398 PE=4 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 8.6e-241
Identity = 416/581 (71.60%), Postives = 494/581 (85.03%), Query Frame = 0

Query: 1   MANDGAAEAWIFIRQVARGRWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNT 60
           M N G +EAW FI+QV  GRWFSVFA  ++MIG G+TY+F TYSK IKT+F+YSQT++NT
Sbjct: 1   MGNKGGSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNVGVFAGLLAEVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFI 120
           LGFAKDLGSN+G+ AGLLAEV P WVLFI+G+  NF  +F+IWLS+T RIAKP+FW MFI
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPS 180
            +C  TNS N+ANTA++VTSVRNFPDRRGIILGLLKG+VGIGGAI++Q YLA+YGH DPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPS 180

Query: 181 NLILLLSWFPSFVSLVLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQKQV 240
           NL+LL +W PS + L+L  +IRPI+IRKHP+ELKV Y LLYVSILLA+FILFLT+ QKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 VFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMVTVP 300
           VFS  GY  GA  +++LL +P+LI  REEL+LYKLNKQ   PSV VS+V  Q  P     
Sbjct: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMV-HQNPP----- 300

Query: 301 QNTPPTSTLQEMTEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGSSLA 360
             T P S   E+ EISPSC ENI NKPERGEDFTILQA+FS DMAL+ LATF+GCGSSLA
Sbjct: 301 --TLPASADSEIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLA 360

Query: 361 SIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFAFSF 420
           +IDN+GQ+GESL YP ++I I VSWVS+FNFFGRVFSGFISETLMTKYKLPRPL F+F+F
Sbjct: 361 AIDNLGQVGESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAF 420

Query: 421 LLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQLVV 480
           L+TC+GQLFIA+P PGSVY+A++IIGFGFGAQ P+LFA+ISE+FGL+RYSTLFNCGQL V
Sbjct: 421 LITCVGQLFIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAV 480

Query: 481 PFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALVALV 540
           P GSYILNVD+VGKLYD+EA++EG K  GKGLTCTGAHCFG SFTILA+ TLFGA+V LV
Sbjct: 481 PLGSYILNVDVVGKLYDMEALREGKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLV 540

Query: 541 LAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNIDD 582
           LAYRTREFYK DVYK + E++WIPQ++MEFYRLD+KK+I+D
Sbjct: 541 LAYRTREFYKRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED 573

BLAST of MS006870 vs. ExPASy TrEMBL
Match: A0A6J1HP70 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464775 PE=4 SV=1)

HSP 1 Score: 842.0 bits (2174), Expect = 1.5e-240
Identity = 416/581 (71.60%), Postives = 492/581 (84.68%), Query Frame = 0

Query: 1   MANDGAAEAWIFIRQVARGRWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNT 60
           M N G +EAW FI+QV  GRWFSVFA  ++MIG G+TY+F TYSK IKT+F+YSQT++NT
Sbjct: 1   MGNKGGSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNVGVFAGLLAEVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFI 120
           LGFAKDLGSN+G+ AGLLAEV P WVLFI+G+  NF  +F+IWLS+T RIAKP+FW MFI
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPS 180
            +C ATNS N+ANTA++VTSVRNFPDRRGIILGLLKG+VGIGGAI++QLYLA+YGH DPS
Sbjct: 121 LVCFATNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPS 180

Query: 181 NLILLLSWFPSFVSLVLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQKQV 240
           NL+LL +W PS + L+L  +IRPI+IRKHPDELKV Y LLYVSILLA+FILFLT+ QKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 VFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMVTVP 300
           VFS  GY  GA  ++ LL +P+LI  REEL+LYKLNKQ   PSV +S++  Q  P     
Sbjct: 241 VFSQAGYVGGASFVVCLLCVPILIACREELLLYKLNKQTAAPSVALSML-HQNPP----- 300

Query: 301 QNTPPTSTLQEMTEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGSSLA 360
             T PTS   E+ EISPSC  NI NKPERGEDFTILQA+FS DMAL+ LATF+GCGSSLA
Sbjct: 301 --TLPTSADSEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLA 360

Query: 361 SIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFAFSF 420
           +IDN+GQ+GESL Y  ++I I VSWVS+FNFFGRVFSGFISETLMTKYKLPRPL FAF+F
Sbjct: 361 AIDNLGQVGESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAF 420

Query: 421 LLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQLVV 480
           L+TC+GQLFIA+P PGS+Y+A++IIGFGFGAQ P+LFA+ISE+FGLKRYSTLFNCGQL  
Sbjct: 421 LITCVGQLFIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAA 480

Query: 481 PFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGALVALV 540
           PFGSYILNVD+VGKLYD+EA++E  +  GKGLTCTGAHCFG SFT+LA+ TLFGA+V  V
Sbjct: 481 PFGSYILNVDVVGKLYDMEALRERKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFV 540

Query: 541 LAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNIDD 582
           LAYRTREFYK DVYK + E++WIPQ+EMEFYRLDNKKNI+D
Sbjct: 541 LAYRTREFYKRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

BLAST of MS006870 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 591.7 bits (1524), Expect = 6.6e-169
Identity = 315/586 (53.75%), Postives = 414/586 (70.65%), Query Frame = 0

Query: 1   MANDGAAEAWIFIRQVARGRWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNT 60
           M ND   E   F+     GRWF VFASF+IM  AG+TY+FGTYSK IK+   Y QT +N 
Sbjct: 1   MGND---ETVRFLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNL 60

Query: 61  LGFAKDLGSNVGVFAGLLAEVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFI 120
           LGF KDLG+NVGV +GL+AEV P W +  +GSA+NF  YFMIWL+VT ++AKP+ W M +
Sbjct: 61  LGFFKDLGANVGVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCL 120

Query: 121 YICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPS 180
           YIC+  NSQNFANT  LVT V+NFP+ RG++LGLLKG+VG+ GAI TQLY A+YGH D  
Sbjct: 121 YICIGANSQNFANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGH-DSK 180

Query: 181 NLILLLSWFPSFVSLVLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQKQV 240
           +LILL++W P+ VSLV +  IR  K+ +  +EL V Y  LY+SI LALF++ + + +KQV
Sbjct: 181 SLILLIAWLPAAVSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQV 240

Query: 241 VFSHTGYASGAFAILALLALPLLITVREELMLYKLNK-QNNDPSVIVSIVGEQKLPMVTV 300
            FS   YA+ A    ALL +PL ++V++EL ++ + K    +PS +     + + P   +
Sbjct: 241 HFSKAAYAASATICCALLFVPLTVSVKQELEVWNMMKLPIEEPSEV-----KVEKPKKEL 300

Query: 301 PQNTPPTSTLQEMTEISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGSSL 360
             +    + +    + + SC   + + P RGED+TILQA+ S DM ++++ATF G GSSL
Sbjct: 301 DLDQDKAAKVNGEEKETKSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSL 360

Query: 361 ASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFAFS 420
            ++DN+GQIGESL YP  +++ FVS VS++N+FGRVFSGF+SE L+ KYKLPRPLM    
Sbjct: 361 TAVDNLGQIGESLGYPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLV 420

Query: 421 FLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQLV 480
            LL+C G L IAFP PGSVY+AS+++GF FGAQ+PLLFAIISELFGLK YSTLFNCGQL 
Sbjct: 421 LLLSCAGHLLIAFPVPGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLA 480

Query: 481 VPFGSYILNVDIVGKLYDIEAIKE-----GSKKTGKGLTCTGAHCFGWSFTILASVTLFG 540
            P GSYILNV + G LYD EA+K+      ++K  K LTC G+ C+   F ILA+VT FG
Sbjct: 481 SPLGSYILNVRVTGMLYDKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFG 540

Query: 541 ALVALVLAYRTREFYKGDVYKKYREDMWIPQSEMEFYRLDNKKNID 581
           ALV+L LA RTREFYKGD+YKK+RE    P+SE E    D++K ++
Sbjct: 541 ALVSLGLAIRTREFYKGDIYKKFRES---PESESELVP-DSRKAVE 573

BLAST of MS006870 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 546.6 bits (1407), Expect = 2.4e-155
Identity = 285/560 (50.89%), Postives = 381/560 (68.04%), Query Frame = 0

Query: 15  QVARGRWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNTLGFAKDLGSNVGVF 74
           Q+  GRWF  F S +IM  AG+TYMFG YS  IK    Y QT +N L F KDLG+NVGV 
Sbjct: 16  QILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVL 75

Query: 75  AGLLAEVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFIYICLATNSQNFANT 134
           AGLL EV P W + ++G+ LNFF YFMIWL+VT RI+KP+ WHM +YIC+  NSQ+FANT
Sbjct: 76  AGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANT 135

Query: 135 AVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPSNLILLLSWFPSFVS 194
             LVT V+NFP+ RG++LG+LKG+VG+ GAI+TQLY A YG ED   LIL++ W P+ VS
Sbjct: 136 GSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYG-EDTKELILMIGWLPAIVS 195

Query: 195 LVLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQKQVVFSHTGYASGAFAI 254
              L  IR +K+++  +ELKV Y+ LY+S+ LA F++ + ++ K   F+ + +   A  +
Sbjct: 196 FAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVV 255

Query: 255 LALLALPLLITVREELMLYKLNK-QNNDPSVIVSIVGEQKLPMVTVPQNTPPTSTLQEMT 314
           + LL LP+++ + EE  L+K  +   NDP+ I  +  + KL       +    S      
Sbjct: 256 IVLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEKPKLDSSEFKDDDGEESKEVVEK 315

Query: 315 EISPSCLENICNKPERGEDFTILQAIFSVDMALVYLATFTGCGSSLASIDNIGQIGESLH 374
             +PSC   + N PERG+D+TILQA+FSVDM +++LAT  G G +L +IDN+GQIG SL 
Sbjct: 316 VKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIGNSLG 375

Query: 375 YPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMFAFSFLLTCIGQLFIAFP 434
           YP +S++ FVS VS++N++GRV SG +SE  + KYK PRPLM     LL+C G L IAF 
Sbjct: 376 YPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIAFN 435

Query: 435 SPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCGQLVVPFGSYILNVDIVG 494
            PG +YVAS+IIGF FGAQ PLLFAIISE+FGLK YSTL+N G +  P GSY+LNV + G
Sbjct: 436 VPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLNVRVAG 495

Query: 495 KLYDIEAIKE-----GSKKTGKGLTCTGAHCFGWSFTILASVTLFGALVALVLAYRTREF 554
            LYD+EA K+      ++  G+ L C G  CF  SF I+A+VTLFG LV++VL  RT++F
Sbjct: 496 YLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVIRTKKF 555

Query: 555 YKGDVYKKYREDMWIPQSEM 569
           YK D+YKK+RE     + EM
Sbjct: 556 YKSDIYKKFREKALAAEMEM 574

BLAST of MS006870 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 296.2 bits (757), Expect = 5.7e-80
Identity = 185/558 (33.15%), Postives = 298/558 (53.41%), Query Frame = 0

Query: 20  RWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNTLGFAKDLGSNVGVFAGLLA 79
           RW    A+  I   AG  Y+FG+ S  IK+  NY+Q E++ LG AKDLG +VG  AG L+
Sbjct: 16  RWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIAGTLS 75

Query: 80  EVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFIYICLATNSQNFANTAVLVT 139
           E+ PLW   +VG+  N   Y  +WL VT R      W M + I +  N + + NT  LV+
Sbjct: 76  EILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTGALVS 135

Query: 140 SVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPSNLILLLSWFPSFVSLVLLL 199
            V+NFP  RG ++G+LKGF G+GGAI++Q+Y  ++   +P++LIL+++  P+ V + L+ 
Sbjct: 136 GVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHS-SNPASLILMVAVTPAVVVVCLMF 195

Query: 200 AIRPIKIRK--HPDELKVLYHLLYVSILLALFILFLTLMQKQVVFSHTGYASGAFAILAL 259
            IRP+   K   P +      +  V +LLA +++ + L+Q  VV SH         +  +
Sbjct: 196 FIRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITVFTIVLFVI 255

Query: 260 LALPLLITVR--------------EELMLYKLNKQN---NDPSVIVSIVGEQKLPMV-TV 319
           L +P+L+ +               EE ++ K   Q      P +I+S V ++K   V  +
Sbjct: 256 LVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLILSEVEDEKPKDVDLL 315

Query: 320 PQNTPPTSTLQEMTEISPSCLE-----NICNKPERGEDFTILQAIFSVDMALVYLATFTG 379
           P +           ++  +  E     N    P RGEDFT+ QA+   D  L++ +   G
Sbjct: 316 PASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLIFFSLLLG 375

Query: 380 CGSSLASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPL 439
            GS L  IDN+GQ+ +SL Y   +  + VS +S++NF GR+  G+ SE ++  Y  PRP+
Sbjct: 376 SGSGLTVIDNLGQMSQSLGY--DNTHVLVSMISIWNFLGRIGGGYFSELVVRDYAYPRPV 435

Query: 440 MFAFSFLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFN 499
             A + L+  +G +F A+  PG++Y+ +L+IG G+GA   ++ A  SELFGLK++  L+N
Sbjct: 436 AMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFGLKKFGALYN 495

Query: 500 CGQLVVPFGSYILNVDIVGKLYDIEAIKEGSKKT---GKGLTCTGAHCFGWSFTILASVT 550
              L  P GS + +  I   +YD EA ++           L C G+ CF  +  I++   
Sbjct: 496 FLTLANPAGSLVFSGMIASSIYDREAERQAHGSVFDPDDALRCNGSICFFLTSLIMSGFC 555

BLAST of MS006870 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 293.1 bits (749), Expect = 4.9e-79
Identity = 203/560 (36.25%), Postives = 307/560 (54.82%), Query Frame = 0

Query: 15  QVARGRWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNTLGFAKDLGSNVGVF 74
           ++ R +W ++ AS  I   +G++Y FG YS  +K+  +Y Q+ ++T+   KD+G+N GVF
Sbjct: 2   EILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVF 61

Query: 75  AGLLAEVAPL----------------WVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHM 134
           +GLL   A                  WV+  VG+   F  YF+IW SVT  I KP    M
Sbjct: 62  SGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLM 121

Query: 135 FIYICLATNSQNFANTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHED 194
            +++ LA  SQ F NTA +V++V NF D  G  +G++KGF+G+ GAIL QLY  +    D
Sbjct: 122 CLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCA-GD 181

Query: 195 PSNLILLLSWFPSFVSLVLLLAIRPIKIRKHPDELKVLYHLLYVSILLALFILFLTLMQK 254
           P++ ILLL+  P+ +SL+++  +R I      D+ K L  L  VS+++A +++ + +++ 
Sbjct: 182 PASFILLLAVTPTVLSLLVMPLVR-IYETSVADDKKHLNGLSAVSLIIAAYLMIIIILKN 241

Query: 255 QVVFSHTGYASGAFAILALLALPLLITVREELMLYKLNKQNNDPSVIVSIVGEQKLPMVT 314
               S          +L +LALPLLI  R +                    G +K    T
Sbjct: 242 TFGLSSWANIVTLVCLLVMLALPLLIARRAQR------------------DGMEK----T 301

Query: 315 VPQNTPPTSTLQEMTEISPSCLENICNKPERG--EDFTILQAIFSVDMALVYLATFTGCG 374
           VP +  P  +  + T       E   +K E G  E+  +LQA+  +   L++LA   G G
Sbjct: 302 VPHDYSPLISSPKATTSGNQSSEG-DSKVEAGLSENLNLLQAMKKLSFWLLFLAMICGMG 361

Query: 375 SSLASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGFISETLMTKYKLPRPLMF 434
           S L++I+NI QIGESL Y +  I   VS  S++NF GR  +G+ S+ L+ K   PRPL+ 
Sbjct: 362 SGLSTINNIRQIGESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLM 421

Query: 435 AFSFLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAIISELFGLKRYSTLFNCG 494
           A +     IG L IA    G++YV S+I+G  +G+Q  L+  I SELFG++   T+FN  
Sbjct: 422 AATLGTMSIGHLIIASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTI 481

Query: 495 QLVVPFGSYILNVDIVGKLYDIEAIKEGSKKTGKGLTCTGAHCFGWSFTILASVTLFGAL 554
            +  P GSYI +V ++G +YD  A       +G+G TC G+HCF  SF I+ASV  FG L
Sbjct: 482 SVASPIGSYIFSVRLIGYIYDKTA-------SGEGNTCYGSHCFRLSFIIMASVAFFGFL 529

Query: 555 VALVLAYRTREFYKGDVYKK 557
           VA+VL +RT+  Y+  + K+
Sbjct: 542 VAIVLFFRTKTLYRQILVKR 529

BLAST of MS006870 vs. TAIR 10
Match: AT3G01930.2 (Major facilitator superfamily protein )

HSP 1 Score: 287.7 bits (735), Expect = 2.0e-77
Identity = 193/582 (33.16%), Postives = 307/582 (52.75%), Query Frame = 0

Query: 13  IRQVARGRWFSVFASFMIMIGAGSTYMFGTYSKAIKTEFNYSQTEVNTLGFAKDLGSNVG 72
           ++     RW    A+  I   AG  Y+FG+ S  IK+  NY+Q +++ LG AKDLG +VG
Sbjct: 9   VKSFINNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKQLSRLGVAKDLGDSVG 68

Query: 73  VFAGLLAEVAPLWVLFIVGSALNFFSYFMIWLSVTRRIAKPEFWHMFIYICLATNSQNFA 132
             AG L+E+ PLW   +VGS  N   Y  +WL VT R      W M I I +  N + + 
Sbjct: 69  FLAGTLSEILPLWAALLVGSVQNLVGYGWVWLIVTGRAPILPLWAMCILIFVGNNGETYF 128

Query: 133 NTAVLVTSVRNFPDRRGIILGLLKGFVGIGGAILTQLYLAVYGHEDPSNLILLLSWFPSF 192
           NTA LV+ V+NFP  RG ++G+LKGF G+GGAIL+Q+Y  ++   D ++LI +++  PS 
Sbjct: 129 NTAALVSGVQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHS-SDRASLIFMVAVAPSV 188

Query: 193 VSLVLLLAIRPI----KIR-KHPDELKVLYHLLYVSILLALFILFLTLMQKQVVFSHTGY 252
           V + L+  IRP+    +IR        V+Y    V ILLA +++ + L++  +  SH+  
Sbjct: 189 VVVPLMFFIRPVGGHRQIRSSDATSFTVIY---AVCILLAAYLMAVMLVEDFIDLSHSII 248

Query: 253 ASGAFAILALLALPLLITVR--------------EELMLYKLNKQ--------NNDPSVI 312
            +    + A+L +P+ I +               EE +L     Q        ++ P +I
Sbjct: 249 IAFTVVLFAILLVPIFIPIATSCFTASTDPCDTLEEPLLGDQQGQDPGQSTTPDHGPELI 308

Query: 313 VSIVGEQKLPMV-TVPQNTPPTSTLQEMTEISPSCLENIC-----NKPERGEDFTILQAI 372
            S V ++K   V  +P         Q   ++  +  E          P RGEDFT+ QA+
Sbjct: 309 FSEVEDEKPKEVDLLPAVERHKRIAQLQAKLMQAAAEGAVRVKRRRGPHRGEDFTLTQAL 368

Query: 373 FSVDMALVYLATFTGCGSSLASIDNIGQIGESLHYPTQSIAIFVSWVSVFNFFGRVFSGF 432
              D  L++ +   G GS L  IDN+GQ+ +SL Y   +  +FVS +S++NF GR+  G+
Sbjct: 369 VKADFWLIFFSLLLGSGSGLTVIDNLGQMSQSLGY--DNTHVFVSMISIWNFLGRIGGGY 428

Query: 433 ISETLMTKYKLPRPLMFAFSFLLTCIGQLFIAFPSPGSVYVASLIIGFGFGAQVPLLFAI 492
            SE ++  Y  PRP+  A + L+  +G +F A+  PG++++ +L+IG G+GA   ++ A 
Sbjct: 429 FSELIVRDYAYPRPVAIAVAQLVMSVGHIFFAYGWPGAMHIGTLLIGLGYGAHWAIVPAT 488

Query: 493 ISELFGLKRYSTLFNCGQLVVPFGSYILNVDIVGKLYDIEAIKEGSK---KTGKGLTCTG 552
            SELFGLK++  L+N   L  P GS + +  I   +YD EA ++           L C G
Sbjct: 489 ASELFGLKKFGALYNFLTLANPAGSLVFSGLIASSIYDREAERQAQGSLFNPDDVLRCRG 548

Query: 553 AHCFGWSFTILASVTLFGALVALVLAYRTREFYKGDVYKKYR 559
           + C+  +  I++   L  A ++++L  RT+  Y  ++Y K R
Sbjct: 549 SICYFLTSLIMSGFCLIAAALSMILVQRTKPVYT-NLYGKTR 583

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152846.11.0e-25699.35uncharacterized protein LOC111020470 [Momordica charantia][more]
XP_022152849.12.9e-24374.52uncharacterized protein LOC111020473 [Momordica charantia][more]
XP_022152845.11.0e-24071.77uncharacterized protein LOC111020469 [Momordica charantia][more]
XP_022970365.11.8e-24071.60protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima] >XP_022970399.1 prote... [more]
XP_022964774.13.0e-24071.60protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
F4I9E15.0e-2825.60Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A6J1DIZ35.0e-25799.35uncharacterized protein LOC111020470 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
A0A6J1DHB01.4e-24374.52uncharacterized protein LOC111020473 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
A0A6J1DF435.0e-24171.77uncharacterized protein LOC111020469 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
A0A6J1I5D78.6e-24171.60protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469... [more]
A0A6J1HP701.5e-24071.60protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT2G28120.16.6e-16953.75Major facilitator superfamily protein [more]
AT2G39210.12.4e-15550.89Major facilitator superfamily protein [more]
AT5G14120.15.7e-8033.15Major facilitator superfamily protein [more]
AT1G74780.14.9e-7936.25Nodulin-like / Major Facilitator Superfamily protein [more]
AT3G01930.22.0e-7733.16Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 320..550
e-value: 6.6E-13
score: 50.5
coord: 14..210
e-value: 8.0E-7
score: 30.6
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 21..549
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 20..265
e-value: 3.3E-79
score: 265.9
NoneNo IPR availablePANTHERPTHR21576:SF29PROTEIN NUCLEAR FUSION DEFECTIVE 4coord: 5..560
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 5..560
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 19..535
e-value: 2.53688E-95
score: 294.544

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS006870.1MS006870.1mRNA