MS006868 (gene) Bitter gourd (TR) v1

Overview
NameMS006868
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionMajor facilitator superfamily protein
Locationscaffold871: 234883 .. 236499 (-)
RNA-Seq ExpressionMS006868
SyntenyMS006868
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CGATTCACGAAACAAGTTGTAGAAGGGCGATGGTTTTCGGTGTTCGCTTCGTTCGTGATCATGATCGGCGCCGGCTCACCCTACCTATTCGGAACATACTCCAAAGTCATAAAGACCCAATTCGATTACAGCCAAACCCAAGTTAACACCTTGGGATTCTCCAAAGATCTCGGCTCCAACCTCGGAGTCTTCGCCGGACTTCTCGCCGAGGTGGCTCCGCCGTGGGTTCTGTTCGTCGTCGGCTCCTCCCTCAACTTCTTCAGCTTCATCATGATTTGGCTCTCCGTCACTCACCGGATCGCCAAGCCCGAGTTCTGGCAGATGTTCCTCTACGTCGGCTTGGCTGCCAACGCACAAAACTTCGCCAACACTGCGGTTATGGTCATCAGCGTTAAAAACTTCCCGGACCGACGAGGGATTATTATTGGCCTTCTCAAGGGCTTCGTCGGCCTCGGAGGGGCCGTCTTGACTCAGATCTATCTAGCCATTTATGGCCACGAAGATCCATCTGATGTGCTTCTTCTGCTCTCATGGTTTCCGTCCGTTATATCCATTGTTCTTTTGTTTTCAATTAGAACAATCAGAGCTCGCAAACATCCAGAAGAGCTTAAAGTGTTTTACCACTTGCTCTACGCATCTATAATTCTAGCCATCTCTATCCTTATTATTACCTTAACCCAAAAACATATTGTTTTCTCCCATGCTGCCTATTTGGGAAGCTTTGCTGTGGTTGTGGCCTTACTGTGTCTGCCCCTTTTGATCGCCATTAAAGAAGAATTCTTGCTCTTCAAGCTCAACAACCAAGTCGTGGATCCATCCGTCAACCTTTCTATTGCCGAGGAGAAGGAATCTTTCCCGTACTCCAATGTTGTAGTGGAAGAAAATTCCCCTTCTAAGCCACAAAGAGGGGAAGATTTCACCATCTTGCAAGCTCTCCTCAGCAAAGACATGGCTCTCATTTTCTTGGCAACCGTTTGTGCATGTGGGTCGTCCATTGCAGCCATCGACAATCTAGGACAGATTGCAGAATCACTTTCCTATCCAGCTCAATCCATAAGCGTCTTCGTTTCTTGGATTTCCATATTCAATTTCTTCGGTCGGGTCTTCTCTGGCTTCATCTCCGAAACGCTAATGACCAAATACAAATTACCTCGTCCTCTCATGTTCGCCCTTGCCCATCTCCTCACCTGCATTGGCATACTCTTCATCGCCTTTCCATATCATGGTTCGGTCTACGTGGCTTCACTGATCATTGGGTTTGGGTTTGGAGCCCAAGTTCCTTTGTTATTCGCCATAATCTCTGATCTATTTGGCCTCAAGCACTACTCAACGCTGCTCAATTGTGGGCAACTGGCGGTGCCGCTTGGATCTTATGTCCTAAATGTGCTTCTAATAGGTAGGATGTATGATATGGAAGCCACTAGAACTGGAAATACCAAGAACGGTAAGGGGCTCACTTGCAGTGGAGCTCATTGCTTCAACGGCTCTTTCACAATTTTGGCAGCAATAACATTGTTTGGAGCAATAAGTTCACTCGCATTGGCTTATAGAACTCGAGGCTTTTACAAAGGCGACATATATCAGAGATACAGAGATGACATGTGGATTACCCAATCC

mRNA sequence

CGATTCACGAAACAAGTTGTAGAAGGGCGATGGTTTTCGGTGTTCGCTTCGTTCGTGATCATGATCGGCGCCGGCTCACCCTACCTATTCGGAACATACTCCAAAGTCATAAAGACCCAATTCGATTACAGCCAAACCCAAGTTAACACCTTGGGATTCTCCAAAGATCTCGGCTCCAACCTCGGAGTCTTCGCCGGACTTCTCGCCGAGGTGGCTCCGCCGTGGGTTCTGTTCGTCGTCGGCTCCTCCCTCAACTTCTTCAGCTTCATCATGATTTGGCTCTCCGTCACTCACCGGATCGCCAAGCCCGAGTTCTGGCAGATGTTCCTCTACGTCGGCTTGGCTGCCAACGCACAAAACTTCGCCAACACTGCGGTTATGGTCATCAGCGTTAAAAACTTCCCGGACCGACGAGGGATTATTATTGGCCTTCTCAAGGGCTTCGTCGGCCTCGGAGGGGCCGTCTTGACTCAGATCTATCTAGCCATTTATGGCCACGAAGATCCATCTGATGTGCTTCTTCTGCTCTCATGGTTTCCGTCCGTTATATCCATTGTTCTTTTGTTTTCAATTAGAACAATCAGAGCTCGCAAACATCCAGAAGAGCTTAAAGTGTTTTACCACTTGCTCTACGCATCTATAATTCTAGCCATCTCTATCCTTATTATTACCTTAACCCAAAAACATATTGTTTTCTCCCATGCTGCCTATTTGGGAAGCTTTGCTGTGGTTGTGGCCTTACTGTGTCTGCCCCTTTTGATCGCCATTAAAGAAGAATTCTTGCTCTTCAAGCTCAACAACCAAGTCGTGGATCCATCCGTCAACCTTTCTATTGCCGAGGAGAAGGAATCTTTCCCGTACTCCAATGTTGTAGTGGAAGAAAATTCCCCTTCTAAGCCACAAAGAGGGGAAGATTTCACCATCTTGCAAGCTCTCCTCAGCAAAGACATGGCTCTCATTTTCTTGGCAACCGTTTGTGCATGTGGGTCGTCCATTGCAGCCATCGACAATCTAGGACAGATTGCAGAATCACTTTCCTATCCAGCTCAATCCATAAGCGTCTTCGTTTCTTGGATTTCCATATTCAATTTCTTCGGTCGGGTCTTCTCTGGCTTCATCTCCGAAACGCTAATGACCAAATACAAATTACCTCGTCCTCTCATGTTCGCCCTTGCCCATCTCCTCACCTGCATTGGCATACTCTTCATCGCCTTTCCATATCATGGTTCGGTCTACGTGGCTTCACTGATCATTGGGTTTGGGTTTGGAGCCCAAGTTCCTTTGTTATTCGCCATAATCTCTGATCTATTTGGCCTCAAGCACTACTCAACGCTGCTCAATTGTGGGCAACTGGCGGTGCCGCTTGGATCTTATGTCCTAAATGTGCTTCTAATAGGTAGGATGTATGATATGGAAGCCACTAGAACTGGAAATACCAAGAACGGTAAGGGGCTCACTTGCAGTGGAGCTCATTGCTTCAACGGCTCTTTCACAATTTTGGCAGCAATAACATTGTTTGGAGCAATAAGTTCACTCGCATTGGCTTATAGAACTCGAGGCTTTTACAAAGGCGACATATATCAGAGATACAGAGATGACATGTGGATTACCCAATCC

Coding sequence (CDS)

CGATTCACGAAACAAGTTGTAGAAGGGCGATGGTTTTCGGTGTTCGCTTCGTTCGTGATCATGATCGGCGCCGGCTCACCCTACCTATTCGGAACATACTCCAAAGTCATAAAGACCCAATTCGATTACAGCCAAACCCAAGTTAACACCTTGGGATTCTCCAAAGATCTCGGCTCCAACCTCGGAGTCTTCGCCGGACTTCTCGCCGAGGTGGCTCCGCCGTGGGTTCTGTTCGTCGTCGGCTCCTCCCTCAACTTCTTCAGCTTCATCATGATTTGGCTCTCCGTCACTCACCGGATCGCCAAGCCCGAGTTCTGGCAGATGTTCCTCTACGTCGGCTTGGCTGCCAACGCACAAAACTTCGCCAACACTGCGGTTATGGTCATCAGCGTTAAAAACTTCCCGGACCGACGAGGGATTATTATTGGCCTTCTCAAGGGCTTCGTCGGCCTCGGAGGGGCCGTCTTGACTCAGATCTATCTAGCCATTTATGGCCACGAAGATCCATCTGATGTGCTTCTTCTGCTCTCATGGTTTCCGTCCGTTATATCCATTGTTCTTTTGTTTTCAATTAGAACAATCAGAGCTCGCAAACATCCAGAAGAGCTTAAAGTGTTTTACCACTTGCTCTACGCATCTATAATTCTAGCCATCTCTATCCTTATTATTACCTTAACCCAAAAACATATTGTTTTCTCCCATGCTGCCTATTTGGGAAGCTTTGCTGTGGTTGTGGCCTTACTGTGTCTGCCCCTTTTGATCGCCATTAAAGAAGAATTCTTGCTCTTCAAGCTCAACAACCAAGTCGTGGATCCATCCGTCAACCTTTCTATTGCCGAGGAGAAGGAATCTTTCCCGTACTCCAATGTTGTAGTGGAAGAAAATTCCCCTTCTAAGCCACAAAGAGGGGAAGATTTCACCATCTTGCAAGCTCTCCTCAGCAAAGACATGGCTCTCATTTTCTTGGCAACCGTTTGTGCATGTGGGTCGTCCATTGCAGCCATCGACAATCTAGGACAGATTGCAGAATCACTTTCCTATCCAGCTCAATCCATAAGCGTCTTCGTTTCTTGGATTTCCATATTCAATTTCTTCGGTCGGGTCTTCTCTGGCTTCATCTCCGAAACGCTAATGACCAAATACAAATTACCTCGTCCTCTCATGTTCGCCCTTGCCCATCTCCTCACCTGCATTGGCATACTCTTCATCGCCTTTCCATATCATGGTTCGGTCTACGTGGCTTCACTGATCATTGGGTTTGGGTTTGGAGCCCAAGTTCCTTTGTTATTCGCCATAATCTCTGATCTATTTGGCCTCAAGCACTACTCAACGCTGCTCAATTGTGGGCAACTGGCGGTGCCGCTTGGATCTTATGTCCTAAATGTGCTTCTAATAGGTAGGATGTATGATATGGAAGCCACTAGAACTGGAAATACCAAGAACGGTAAGGGGCTCACTTGCAGTGGAGCTCATTGCTTCAACGGCTCTTTCACAATTTTGGCAGCAATAACATTGTTTGGAGCAATAAGTTCACTCGCATTGGCTTATAGAACTCGAGGCTTTTACAAAGGCGACATATATCAGAGATACAGAGATGACATGTGGATTACCCAATCC

Protein sequence

RFTKQVVEGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSNLGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQNFANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLSWFPSVISIVLLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIVFSHAAYLGSFAVVVALLCLPLLIAIKEEFLLFKLNNQVVDPSVNLSIAEEKESFPYSNVVVEENSPSKPQRGEDFTILQALLSKDMALIFLATVCACGSSIAAIDNLGQIAESLSYPAQSISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHGSVYVASLIIGFGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYVLNVLLIGRMYDMEATRTGNTKNGKGLTCSGAHCFNGSFTILAAITLFGAISSLALAYRTRGFYKGDIYQRYRDDMWITQS
Homology
BLAST of MS006868 vs. NCBI nr
Match: XP_022152848.1 (uncharacterized membrane protein YMR155W-like [Momordica charantia])

HSP 1 Score: 976.1 bits (2522), Expect = 1.2e-280
Identity = 506/543 (93.19%), Postives = 522/543 (96.13%), Query Frame = 0

Query: 1   RFTKQVVEGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSN 60
           RF KQV +GRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSN
Sbjct: 11  RFAKQVADGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSN 70

Query: 61  LGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQN 120
           LGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQN
Sbjct: 71  LGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQN 130

Query: 121 FANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLSWFP 180
           FANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLSWFP
Sbjct: 131 FANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLSWFP 190

Query: 181 SVISIVLLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIVFSHAAYLGS 240
           SVISIVLLFSIRTIRAR HPEELKVFYHLLYASIILAISILIITLTQKHIVFSHAAYLGS
Sbjct: 191 SVISIVLLFSIRTIRARXHPEELKVFYHLLYASIILAISILIITLTQKHIVFSHAAYLGS 250

Query: 241 FAVVVALLCLPLLIAIKEEFLLFKLNNQVVDPSVNLSIAEEKESFPYSNV----VVEENS 300
            AVVVALLCLPLLIAIKEEFLLFKLNNQ VDPSV LSI EEK+S PYSN+    V+E++S
Sbjct: 251 VAVVVALLCLPLLIAIKEEFLLFKLNNQAVDPSVTLSIPEEKQSLPYSNILLSTVLEKDS 310

Query: 301 PSKPQRGEDFTILQALLSKDMALIFLATVCACGSSIAAIDNLGQIAESLSYPAQSISVFV 360
           PSKP+RGEDFTILQALLSKDMAL+FLATV ACGSSI AIDNLGQIAESL+YPAQSISVFV
Sbjct: 311 PSKPERGEDFTILQALLSKDMALVFLATVSACGSSIVAIDNLGQIAESLAYPAQSISVFV 370

Query: 361 SWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHGSVYVASL 420
           SWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHGSVYVASL
Sbjct: 371 SWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHGSVYVASL 430

Query: 421 IIGFGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYVLNVLLIGRMYDMEATRT 480
           IIG GFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSY+LNVLL+GRMYDMEA R 
Sbjct: 431 IIGSGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYILNVLLVGRMYDMEANRA 490

Query: 481 GNTKNGKGLTCSGAHCFNGSFTILAAITLFGAISSLALAYRTRGFYKGDIYQRYRDDMWI 540
           GNTK+GKGLTC+G HCF GSFTILAAITLFGA+SSLALA RTR FYKGDIY+RYRDDMWI
Sbjct: 491 GNTKHGKGLTCNGPHCFGGSFTILAAITLFGAMSSLALACRTRNFYKGDIYKRYRDDMWI 550

BLAST of MS006868 vs. NCBI nr
Match: XP_022152849.1 (uncharacterized protein LOC111020473 [Momordica charantia])

HSP 1 Score: 793.9 bits (2049), Expect = 8.7e-226
Identity = 404/542 (74.54%), Postives = 464/542 (85.61%), Query Frame = 0

Query: 2   FTKQVVEGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSNL 61
           F KQV EGRWFSVFASF+IMIGAGS YLFGTYSK++KTQFDY QTQVNTLGF+KDLGSN 
Sbjct: 13  FVKQVAEGRWFSVFASFLIMIGAGSTYLFGTYSKLLKTQFDYDQTQVNTLGFAKDLGSNF 72

Query: 62  GVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQNF 121
           GVFAGLLAEVAP WVLF+VGSSLNFFS+ MIWLSVT RIA P+FW M LY+ LAAN+QNF
Sbjct: 73  GVFAGLLAEVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMCLYICLAANSQNF 132

Query: 122 ANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLSWFPS 181
           ANTAV+V SVKNFP+RRGII+GLLKGFVG+GGA+LTQ+YLAIYGHE+ + V+LLL+WFPS
Sbjct: 133 ANTAVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHENQASVILLLAWFPS 192

Query: 182 VISIVLLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIVFSHAAYLGSF 241
           VIS V + SIRTI+ RKHPEELKVFYHLLY SIILA+ IL +TLTQK  VFSHA Y+G  
Sbjct: 193 VISFVFVLSIRTIKVRKHPEELKVFYHLLYVSIILALYILFLTLTQKQAVFSHAGYVGGA 252

Query: 242 AVVVALLCLPLLIAIKEEFLLFKLNNQVVDPSVNLSIAEEKESFPYSNVVVEENS----P 301
           +V+V LLCLPLLIAI+EEF+LF LN Q  D  V + I ++K   P     +EE++     
Sbjct: 253 SVIVCLLCLPLLIAIREEFMLFNLNKQNQDGHVAVLIHDQK---PPPKRALEEDAIDSVS 312

Query: 302 SKPQRGEDFTILQALLSKDMALIFLATVCACGSSIAAIDNLGQIAESLSYPAQSISVFVS 361
            KP+RG+DFTI QALLSKDMAL+F+AT+  CGSSIAAIDN+GQIAESL YP  SI++FVS
Sbjct: 313 IKPERGDDFTIFQALLSKDMALLFIATLSGCGSSIAAIDNIGQIAESLQYPPHSIAIFVS 372

Query: 362 WISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHGSVYVASLI 421
           W+SIFNFFGRVFSGFISETLM KYKLPRPLMFA + LLTCIG L IAFPY GSVY ASLI
Sbjct: 373 WVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLLTCIGQLLIAFPYRGSVYAASLI 432

Query: 422 IGFGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYVLNVLLIGRMYDMEATRTG 481
           IGFGFGAQVPLLFAIIS+LFGLKHYSTL NCGQLAVP GSY+LNV +IG++YDMEA R G
Sbjct: 433 IGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPFGSYILNVDVIGKLYDMEAIRQG 492

Query: 482 NTKNGKGLTCSGAHCFNGSFTILAAITLFGAISSLALAYRTRGFYKGDIYQRYRDDMWIT 540
             K GKGLTC+GA+CF+ SFTILAA+T FGA+SSL LAYRTR FYKG+ Y++YR+DMWI 
Sbjct: 493 GKKTGKGLTCTGANCFSNSFTILAAVTFFGALSSLVLAYRTRDFYKGETYKKYREDMWIP 551

BLAST of MS006868 vs. NCBI nr
Match: XP_022152845.1 (uncharacterized protein LOC111020469 [Momordica charantia])

HSP 1 Score: 785.4 bits (2027), Expect = 3.1e-223
Identity = 387/544 (71.14%), Postives = 469/544 (86.21%), Query Frame = 0

Query: 2   FTKQVVEGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSNL 61
           F +QVVEGRWFSVFASF+IMIGAGS Y+FGTYSKV+K QFDYSQTQVNTLGF KDLGSNL
Sbjct: 12  FVRQVVEGRWFSVFASFLIMIGAGSTYVFGTYSKVMKAQFDYSQTQVNTLGFFKDLGSNL 71

Query: 62  GVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQNF 121
           GVFAGLLAEVAPPWVLFVVGSSLNF S+ MIWLS+THRIAKPEFW M  Y+ LAAN+QNF
Sbjct: 72  GVFAGLLAEVAPPWVLFVVGSSLNFVSYFMIWLSLTHRIAKPEFWHMCTYICLAANSQNF 131

Query: 122 ANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLSWFPS 181
           ANTAV+V SVKNFP+RRGII+GLLKGFVG+GGA+LTQ+YLAIYGHE+  +++LLLSWFP+
Sbjct: 132 ANTAVLVTSVKNFPERRGIILGLLKGFVGVGGAILTQMYLAIYGHENQVNLVLLLSWFPT 191

Query: 182 VISIVLLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIVFSHAAYLGSF 241
           +IS+V L +IR I+ RKHP+EL+V YHLLY SIILA+ +L +T+TQK  VFS   Y+   
Sbjct: 192 IISLVFLLTIRPIKVRKHPDELRVMYHLLYVSIILALFLLFLTITQKQAVFSQEGYMSGA 251

Query: 242 AVVVALLCLPLLIAIKEEFLLFKLNNQVVDPSVNLSIAEEKESFPYSNVVVEENSP---- 301
           AV+ +LL +PLLIA++EEFLLFKLN +  +PSV +SI +++   P  + +VE        
Sbjct: 252 AVIASLLSIPLLIAVREEFLLFKLNKETTNPSVTVSILDQQRPAPMLDEIVEAEPSCMSS 311

Query: 302 --SKPQRGEDFTILQALLSKDMALIFLATVCACGSSIAAIDNLGQIAESLSYPAQSISVF 361
             +KPQRG+D+TILQAL SKDMALIF+  +C CGSSIAAIDNLGQI ESL YPAQSI++F
Sbjct: 312 VFNKPQRGDDYTILQALFSKDMALIFVGALCGCGSSIAAIDNLGQIGESLYYPAQSITIF 371

Query: 362 VSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHGSVYVAS 421
           +S ISIFNFFGRV SGFISETLMTK+KLPRPLMFALAHLLTCIG+LF+AFP+HGS+Y+AS
Sbjct: 372 ISLISIFNFFGRVCSGFISETLMTKHKLPRPLMFALAHLLTCIGLLFVAFPFHGSIYIAS 431

Query: 422 LIIGFGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYVLNVLLIGRMYDMEATR 481
           +++GFGFGAQVP++FAIIS+LFGLK Y+TL N GQL+VP+GSY+LNV +IG++YDMEA +
Sbjct: 432 VVVGFGFGAQVPMVFAIISELFGLKRYATLFNFGQLSVPIGSYILNVDIIGKLYDMEARK 491

Query: 482 TGNTKNGKGLTCSGAHCFNGSFTILAAITLFGAISSLALAYRTRGFYKGDIYQRYRDDMW 540
            G TKNGKGLTC+G  CF+ SFTILA +TLFGA++SL LA+RTR FYKGD+Y +YR+DMW
Sbjct: 492 QGTTKNGKGLTCTGHECFSTSFTILATVTLFGALTSLVLAFRTRDFYKGDVYNKYREDMW 551

BLAST of MS006868 vs. NCBI nr
Match: XP_038895745.1 (uncharacterized protein LOC120083909 [Benincasa hispida])

HSP 1 Score: 777.3 bits (2006), Expect = 8.5e-221
Identity = 390/542 (71.96%), Postives = 458/542 (84.50%), Query Frame = 0

Query: 1   RFTKQVVEGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSN 60
           RF KQVVEGRWFSVFA+F+IMIG GS YLFGTYSKV+KT+FDY+QTQ++ LGF+KDLGSN
Sbjct: 11  RFVKQVVEGRWFSVFAAFLIMIGCGSTYLFGTYSKVLKTKFDYNQTQLSHLGFAKDLGSN 70

Query: 61  LGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQN 120
           LGVFAGL AEVAPPW+LF+VG +LNFFS+ MIWLSVT  + KPE W MF+Y+ ++ANAQN
Sbjct: 71  LGVFAGLFAEVAPPWMLFLVGLTLNFFSYFMIWLSVTEYVPKPELWLMFIYIYISANAQN 130

Query: 121 FANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLSWFP 180
           FANTAVMV SV+NFPD+RGII+GLLKGFVGLGGA+LTQIY AIYGH+DP +++LLLSW P
Sbjct: 131 FANTAVMVTSVRNFPDQRGIILGLLKGFVGLGGAILTQIYFAIYGHQDPINLVLLLSWLP 190

Query: 181 SVISIVLLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIVFSHAAYLGS 240
           S+I  +   S RTI+ARKHP+ELKVF+HLLY SI +A+ IL +T+TQKH  F+HA Y+G 
Sbjct: 191 SIICFLFFLSFRTIKARKHPQELKVFFHLLYVSITMAVFILFLTITQKHTPFTHANYVGG 250

Query: 241 FAVVVALLCLPLLIAIKEEFLLFKLNNQVVDPSVNLSIAEEK--ESFPYSNVVVEENSPS 300
            +V+V LLCLPLLIAIKEE  LFKLN Q  DPSV +SI  +K  E    S+        +
Sbjct: 251 TSVIVVLLCLPLLIAIKEELFLFKLNKQTKDPSVVVSIPVQKLEEISETSSPCFSNKRSN 310

Query: 301 KPQRGEDFTILQALLSKDMALIFLATVCACGSSIAAIDNLGQIAESLSYPAQSISVFVSW 360
           KPQRGEDF I+QAL SKDMALIF+ATV ACGSS+AAIDNLGQIAESL+YP+++I+VFVSW
Sbjct: 311 KPQRGEDFGIVQALFSKDMALIFIATVSACGSSVAAIDNLGQIAESLNYPSETINVFVSW 370

Query: 361 ISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHGSVYVASLII 420
           ISIFNFFGRV SGF+SETLMTKYKLPRPLMF L  ++T IG++ IAFPY  SVY ASLII
Sbjct: 371 ISIFNFFGRVCSGFVSETLMTKYKLPRPLMFGLTQVITSIGLISIAFPYKDSVYAASLII 430

Query: 421 GFGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYVLNVLLIGRMYDMEATRTGN 480
           GFGFGAQ PLLFA+ISDLFGLKHYSTLLNCGQLAVP GSY++NV ++G+ YD EAT+ GN
Sbjct: 431 GFGFGAQTPLLFALISDLFGLKHYSTLLNCGQLAVPFGSYIMNVHVVGKFYDREATKIGN 490

Query: 481 TKNGKGLTCSGAHCFNGSFTILAAITLFGAISSLALAYRTRGFYKGDIYQRYRDD-MWIT 540
            KNGKGLTC+G HCF+ SF IL  +TLFGA++S  LAYRTR FYKGDIY+RYRDD MW T
Sbjct: 491 VKNGKGLTCTGTHCFSESFQILVLVTLFGAMTSFVLAYRTREFYKGDIYKRYRDDQMWST 550

BLAST of MS006868 vs. NCBI nr
Match: KAG6583549.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 776.2 bits (2003), Expect = 1.9e-220
Identity = 385/550 (70.00%), Postives = 468/550 (85.09%), Query Frame = 0

Query: 1   RFTKQVVEGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSN 60
           RF KQVVEGRWF+VFASF+IMIGAGS Y+FGTYSK IK+QFDY+QTQ+NTLGF+KDLGSN
Sbjct: 11  RFIKQVVEGRWFAVFASFLIMIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSN 70

Query: 61  LGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQN 120
            GVFAGLL EVAPPWVLF+VGS+LNF+S+ MIWLS+T RI+KP FW+MF+++ +AAN+QN
Sbjct: 71  FGVFAGLLGEVAPPWVLFLVGSALNFWSYFMIWLSITGRISKPPFWEMFVFICVAANSQN 130

Query: 121 FANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLSWFP 180
           FANTAV+V SV+NFPDRRGII+GLLKGFVG GGA+LTQ YLA+YGH++P++++LLLSW P
Sbjct: 131 FANTAVLVTSVRNFPDRRGIILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLP 190

Query: 181 SVISIVLLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIVFSHAAYLGS 240
           +V S++   SIR I+ RK P+EL+V YHLLY SIILA+ +L +T+TQK  VF+  AY+  
Sbjct: 191 TVASMLFFLSIRPIKVRKDPDELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSG 250

Query: 241 FAVVVALLCLPLLIAIKEEFLLFKLNNQVV--DPSVNLSIAEEKES--FPYSNVVVEENS 300
             V++ LL +PLLIAI+EEF+LFKLN Q +  +P+V + + E + S   P +   + E S
Sbjct: 251 ATVIIGLLLIPLLIAIREEFMLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQKIPEAS 310

Query: 301 PS-------KPQRGEDFTILQALLSKDMALIFLATVCACGSSIAAIDNLGQIAESLSYPA 360
            S       KPQRGEDFTILQAL SKDMAL+F+AT+ ACG+SIAAIDNLGQ+ ESL YP+
Sbjct: 311 TSCFSMTFKKPQRGEDFTILQALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPS 370

Query: 361 QSISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHG 420
           Q+IS+FVSW+SIFNFFGRVFSGFISE LMTKYKLPRPLMFA +HLLTCIGILFIAFPY G
Sbjct: 371 QTISIFVSWVSIFNFFGRVFSGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKG 430

Query: 421 SVYVASLIIGFGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYVLNVLLIGRMY 480
           SVY ASLIIGFGFGAQVP+LFAIIS+LFGLK+YST+ NCGQLAVP+GSY+LNV +IG++Y
Sbjct: 431 SVYAASLIIGFGFGAQVPMLFAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLY 490

Query: 481 DMEATRTGNTKNGKGLTCSGAHCFNGSFTILAAITLFGAISSLALAYRTRGFYKGDIYQR 540
           D+EA + G  KNGKGLTC+G HCF+GSF ILA + LFGA++SL LAYRTR FYKGD+Y R
Sbjct: 491 DIEAAKLGGVKNGKGLTCTGTHCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGDVYNR 550

BLAST of MS006868 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 3.0e-27
Identity = 132/536 (24.63%), Postives = 246/536 (45.90%), Query Frame = 0

Query: 10  RWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSNLGVFAGLLA 69
           +W  + A+  I    G+ + F  YS  +K+    SQ ++N L  + DLG   G  +G+  
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 70  EVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQNFANTAVMVI 129
              P  V+    +++ F  + + WL +T+ I  P +  +FL   LA  +  + NTA  ++
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP-YSLVFLCCLLAGLSICWFNTACFIL 162

Query: 130 SVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSD---VLLLLSWFPSVISIV 189
            +++FP+ R + + L   F G+  A+ +  + AI    +PS     LLL S  P V+S  
Sbjct: 163 CIRHFPNNRALALSLTVSFNGISAALYSLAFNAI----NPSSSNLYLLLNSLVPLVVSFA 222

Query: 190 LLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIVFSHAA-------YLG 249
            L+ + T   +   +    +    + S +  I  ++  +T  H++ S ++       ++G
Sbjct: 223 ALYPVLT---KPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIG 282

Query: 250 SFAVVVALLCLPLLIAIKEEFLLF---KLNNQV-------VDPSVNLSIA-EEKESFPYS 309
           +  ++V  LC PLL+  ++ FL     +LN++        +D   N   +   K  + + 
Sbjct: 283 AVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHM 342

Query: 310 NVVVEENSPSKPQRGEDFTILQALLSKDMALIFLATVCACGSSIAAIDNLGQIAESLSYP 369
               E N+    + G++ +    +   +  L ++A  C     +   +NLGQIA+SL   
Sbjct: 343 GTAKEGNT---VRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSL--- 402

Query: 370 AQSISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIG--ILFIAFP 429
            Q+ +  V+  S F+FFGR+ S    + +  +++L R   FA+A L T I   +L ++  
Sbjct: 403 GQNSTTLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSS 462

Query: 430 YHGSVYVASLIIGFGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYVLNVLLIG 489
              ++  A+ +IG   G       +I SDLFG        N     +P+GS +L   +  
Sbjct: 463 QQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGS-LLYGYIAA 522

Query: 490 RMYDMEATRTGNTKNGKGLTCSGAHCFNGSFTILAAITLFGAISSLALAYRTRGFY 523
            +Y+  A+          + C G  C+  +F     +++ G +SSL+L  RT+  Y
Sbjct: 523 SIYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of MS006868 vs. ExPASy Swiss-Prot
Match: Q03795 (Uncharacterized membrane protein YMR155W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YMR155W PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 4.1e-08
Identity = 123/537 (22.91%), Postives = 216/537 (40.22%), Query Frame = 0

Query: 19  VIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSNL-GVFAGLLAEVAPPWVL 78
           V+ +GAG+PYLF  Y+  + ++     +  + L FS  +GS+L G+ AG++ + +P  + 
Sbjct: 27  VVALGAGTPYLFSFYAPQLLSKCHIPVSASSKLSFSLTIGSSLMGILAGIVVDRSPK-LS 86

Query: 79  FVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQNFANTAVMVISVK----N 138
            ++GS   F +++++ L   H       W     + L+     + + +    SVK    N
Sbjct: 87  CLIGSMCVFIAYLILNLCYKHE------WSSTFLISLSLVLIGYGSVSGFYASVKCANTN 146

Query: 139 FPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLSWFPSVISIVLLFSIRT 198
           FP  RG          GL G V + +   ++G E+   V + L      + +V  FS+  
Sbjct: 147 FPQHRGTAGAFPVSLYGLSGMVFSYLCSKLFG-ENIEHVFIFLMVACGCMILVGYFSL-D 206

Query: 199 IRARKHPEELKVFYHLLYASIILAISILIITLTQKHIVFSHAAYLGSFAVVVALLCLPLL 258
           I +    ++  +    L  S        I+ L +    +  +    S         L   
Sbjct: 207 IFSNAEGDDASIKEWELQKS--RETDDNIVPLYENSNDYIGSPVRSSSPATYETYALSDN 266

Query: 259 IAIKEEFLLFKLNNQVVDPSVNLSIAEEKESFPYSNV---VVEENSPSKPQRGEDFTILQ 318
                EF   +       P ++ S    K  F   N     V ENS  K  R     + Q
Sbjct: 267 FQETSEFFALEDRQLSNRPLLSPSSPHTKYDFEDENTSKNTVGENSAQKSMR---LHVFQ 326

Query: 319 ALLSKDMALIFLATVCACGSSIAAIDNLGQIAES---------LSYPAQSI-SVFVSWIS 378
           +L S      ++      G  +  I ++G + ++         L   A+ I S+ V+ +S
Sbjct: 327 SLKSSTFIGYYIVLGILQGVGLMYIYSVGFMVQAQVSTPPLNQLPINAEKIQSLQVTLLS 386

Query: 379 IFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHG----------- 438
           + +F GR+ SG IS+ L+ K+K  R     +A LL  +    I+  +             
Sbjct: 387 LLSFCGRLSSGPISDFLVKKFKAQRLWNIVIASLLVFLASNKISHDFSSIEDPSLRASKS 446

Query: 439 --SVYVASLIIGFGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYVLNVL--LI 498
             ++ V S I G+ FG       +I++D FG   YSTL     +    G + ++V   ++
Sbjct: 447 FKNISVCSAIFGYSFGVLFGTFPSIVADRFGTNGYSTLWG---VLTTGGVFSVSVFTDIL 506

Query: 499 GRMYDMEATRTGNTKNGKGLTCSGAHCFNGSFTI---LAAITLFGAISSLALAYRTR 520
           GR  D +A    NT +  G    G  C++ +F +    AA  L   +  +   Y  R
Sbjct: 507 GR--DFKA----NTGDDDGNCKKGVLCYSYTFMVTKYCAAFNLLFVLGIIGYTYYRR 540

BLAST of MS006868 vs. ExPASy TrEMBL
Match: A0A6J1DG00 (uncharacterized membrane protein YMR155W-like OS=Momordica charantia OX=3673 GN=LOC111020471 PE=4 SV=1)

HSP 1 Score: 976.1 bits (2522), Expect = 6.0e-281
Identity = 506/543 (93.19%), Postives = 522/543 (96.13%), Query Frame = 0

Query: 1   RFTKQVVEGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSN 60
           RF KQV +GRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSN
Sbjct: 11  RFAKQVADGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSN 70

Query: 61  LGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQN 120
           LGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQN
Sbjct: 71  LGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQN 130

Query: 121 FANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLSWFP 180
           FANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLSWFP
Sbjct: 131 FANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLSWFP 190

Query: 181 SVISIVLLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIVFSHAAYLGS 240
           SVISIVLLFSIRTIRAR HPEELKVFYHLLYASIILAISILIITLTQKHIVFSHAAYLGS
Sbjct: 191 SVISIVLLFSIRTIRARXHPEELKVFYHLLYASIILAISILIITLTQKHIVFSHAAYLGS 250

Query: 241 FAVVVALLCLPLLIAIKEEFLLFKLNNQVVDPSVNLSIAEEKESFPYSNV----VVEENS 300
            AVVVALLCLPLLIAIKEEFLLFKLNNQ VDPSV LSI EEK+S PYSN+    V+E++S
Sbjct: 251 VAVVVALLCLPLLIAIKEEFLLFKLNNQAVDPSVTLSIPEEKQSLPYSNILLSTVLEKDS 310

Query: 301 PSKPQRGEDFTILQALLSKDMALIFLATVCACGSSIAAIDNLGQIAESLSYPAQSISVFV 360
           PSKP+RGEDFTILQALLSKDMAL+FLATV ACGSSI AIDNLGQIAESL+YPAQSISVFV
Sbjct: 311 PSKPERGEDFTILQALLSKDMALVFLATVSACGSSIVAIDNLGQIAESLAYPAQSISVFV 370

Query: 361 SWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHGSVYVASL 420
           SWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHGSVYVASL
Sbjct: 371 SWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHGSVYVASL 430

Query: 421 IIGFGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYVLNVLLIGRMYDMEATRT 480
           IIG GFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSY+LNVLL+GRMYDMEA R 
Sbjct: 431 IIGSGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYILNVLLVGRMYDMEANRA 490

Query: 481 GNTKNGKGLTCSGAHCFNGSFTILAAITLFGAISSLALAYRTRGFYKGDIYQRYRDDMWI 540
           GNTK+GKGLTC+G HCF GSFTILAAITLFGA+SSLALA RTR FYKGDIY+RYRDDMWI
Sbjct: 491 GNTKHGKGLTCNGPHCFGGSFTILAAITLFGAMSSLALACRTRNFYKGDIYKRYRDDMWI 550

BLAST of MS006868 vs. ExPASy TrEMBL
Match: A0A6J1DHB0 (uncharacterized protein LOC111020473 OS=Momordica charantia OX=3673 GN=LOC111020473 PE=4 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 4.2e-226
Identity = 404/542 (74.54%), Postives = 464/542 (85.61%), Query Frame = 0

Query: 2   FTKQVVEGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSNL 61
           F KQV EGRWFSVFASF+IMIGAGS YLFGTYSK++KTQFDY QTQVNTLGF+KDLGSN 
Sbjct: 13  FVKQVAEGRWFSVFASFLIMIGAGSTYLFGTYSKLLKTQFDYDQTQVNTLGFAKDLGSNF 72

Query: 62  GVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQNF 121
           GVFAGLLAEVAP WVLF+VGSSLNFFS+ MIWLSVT RIA P+FW M LY+ LAAN+QNF
Sbjct: 73  GVFAGLLAEVAPSWVLFLVGSSLNFFSYFMIWLSVTRRIANPQFWHMCLYICLAANSQNF 132

Query: 122 ANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLSWFPS 181
           ANTAV+V SVKNFP+RRGII+GLLKGFVG+GGA+LTQ+YLAIYGHE+ + V+LLL+WFPS
Sbjct: 133 ANTAVLVTSVKNFPERRGIILGLLKGFVGIGGAILTQMYLAIYGHENQASVILLLAWFPS 192

Query: 182 VISIVLLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIVFSHAAYLGSF 241
           VIS V + SIRTI+ RKHPEELKVFYHLLY SIILA+ IL +TLTQK  VFSHA Y+G  
Sbjct: 193 VISFVFVLSIRTIKVRKHPEELKVFYHLLYVSIILALYILFLTLTQKQAVFSHAGYVGGA 252

Query: 242 AVVVALLCLPLLIAIKEEFLLFKLNNQVVDPSVNLSIAEEKESFPYSNVVVEENS----P 301
           +V+V LLCLPLLIAI+EEF+LF LN Q  D  V + I ++K   P     +EE++     
Sbjct: 253 SVIVCLLCLPLLIAIREEFMLFNLNKQNQDGHVAVLIHDQK---PPPKRALEEDAIDSVS 312

Query: 302 SKPQRGEDFTILQALLSKDMALIFLATVCACGSSIAAIDNLGQIAESLSYPAQSISVFVS 361
            KP+RG+DFTI QALLSKDMAL+F+AT+  CGSSIAAIDN+GQIAESL YP  SI++FVS
Sbjct: 313 IKPERGDDFTIFQALLSKDMALLFIATLSGCGSSIAAIDNIGQIAESLQYPPHSIAIFVS 372

Query: 362 WISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHGSVYVASLI 421
           W+SIFNFFGRVFSGFISETLM KYKLPRPLMFA + LLTCIG L IAFPY GSVY ASLI
Sbjct: 373 WVSIFNFFGRVFSGFISETLMVKYKLPRPLMFASSFLLTCIGQLLIAFPYRGSVYAASLI 432

Query: 422 IGFGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYVLNVLLIGRMYDMEATRTG 481
           IGFGFGAQVPLLFAIIS+LFGLKHYSTL NCGQLAVP GSY+LNV +IG++YDMEA R G
Sbjct: 433 IGFGFGAQVPLLFAIISELFGLKHYSTLFNCGQLAVPFGSYILNVDVIGKLYDMEAIRQG 492

Query: 482 NTKNGKGLTCSGAHCFNGSFTILAAITLFGAISSLALAYRTRGFYKGDIYQRYRDDMWIT 540
             K GKGLTC+GA+CF+ SFTILAA+T FGA+SSL LAYRTR FYKG+ Y++YR+DMWI 
Sbjct: 493 GKKTGKGLTCTGANCFSNSFTILAAVTFFGALSSLVLAYRTRDFYKGETYKKYREDMWIP 551

BLAST of MS006868 vs. ExPASy TrEMBL
Match: A0A6J1DF43 (uncharacterized protein LOC111020469 OS=Momordica charantia OX=3673 GN=LOC111020469 PE=4 SV=1)

HSP 1 Score: 785.4 bits (2027), Expect = 1.5e-223
Identity = 387/544 (71.14%), Postives = 469/544 (86.21%), Query Frame = 0

Query: 2   FTKQVVEGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSNL 61
           F +QVVEGRWFSVFASF+IMIGAGS Y+FGTYSKV+K QFDYSQTQVNTLGF KDLGSNL
Sbjct: 12  FVRQVVEGRWFSVFASFLIMIGAGSTYVFGTYSKVMKAQFDYSQTQVNTLGFFKDLGSNL 71

Query: 62  GVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQNF 121
           GVFAGLLAEVAPPWVLFVVGSSLNF S+ MIWLS+THRIAKPEFW M  Y+ LAAN+QNF
Sbjct: 72  GVFAGLLAEVAPPWVLFVVGSSLNFVSYFMIWLSLTHRIAKPEFWHMCTYICLAANSQNF 131

Query: 122 ANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLSWFPS 181
           ANTAV+V SVKNFP+RRGII+GLLKGFVG+GGA+LTQ+YLAIYGHE+  +++LLLSWFP+
Sbjct: 132 ANTAVLVTSVKNFPERRGIILGLLKGFVGVGGAILTQMYLAIYGHENQVNLVLLLSWFPT 191

Query: 182 VISIVLLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIVFSHAAYLGSF 241
           +IS+V L +IR I+ RKHP+EL+V YHLLY SIILA+ +L +T+TQK  VFS   Y+   
Sbjct: 192 IISLVFLLTIRPIKVRKHPDELRVMYHLLYVSIILALFLLFLTITQKQAVFSQEGYMSGA 251

Query: 242 AVVVALLCLPLLIAIKEEFLLFKLNNQVVDPSVNLSIAEEKESFPYSNVVVEENSP---- 301
           AV+ +LL +PLLIA++EEFLLFKLN +  +PSV +SI +++   P  + +VE        
Sbjct: 252 AVIASLLSIPLLIAVREEFLLFKLNKETTNPSVTVSILDQQRPAPMLDEIVEAEPSCMSS 311

Query: 302 --SKPQRGEDFTILQALLSKDMALIFLATVCACGSSIAAIDNLGQIAESLSYPAQSISVF 361
             +KPQRG+D+TILQAL SKDMALIF+  +C CGSSIAAIDNLGQI ESL YPAQSI++F
Sbjct: 312 VFNKPQRGDDYTILQALFSKDMALIFVGALCGCGSSIAAIDNLGQIGESLYYPAQSITIF 371

Query: 362 VSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHGSVYVAS 421
           +S ISIFNFFGRV SGFISETLMTK+KLPRPLMFALAHLLTCIG+LF+AFP+HGS+Y+AS
Sbjct: 372 ISLISIFNFFGRVCSGFISETLMTKHKLPRPLMFALAHLLTCIGLLFVAFPFHGSIYIAS 431

Query: 422 LIIGFGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYVLNVLLIGRMYDMEATR 481
           +++GFGFGAQVP++FAIIS+LFGLK Y+TL N GQL+VP+GSY+LNV +IG++YDMEA +
Sbjct: 432 VVVGFGFGAQVPMVFAIISELFGLKRYATLFNFGQLSVPIGSYILNVDIIGKLYDMEARK 491

Query: 482 TGNTKNGKGLTCSGAHCFNGSFTILAAITLFGAISSLALAYRTRGFYKGDIYQRYRDDMW 540
            G TKNGKGLTC+G  CF+ SFTILA +TLFGA++SL LA+RTR FYKGD+Y +YR+DMW
Sbjct: 492 QGTTKNGKGLTCTGHECFSTSFTILATVTLFGALTSLVLAFRTRDFYKGDVYNKYREDMW 551

BLAST of MS006868 vs. ExPASy TrEMBL
Match: A0A6J1HYK5 (uncharacterized protein LOC111469251 OS=Cucurbita maxima OX=3661 GN=LOC111469251 PE=4 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 1.6e-220
Identity = 379/550 (68.91%), Postives = 466/550 (84.73%), Query Frame = 0

Query: 1   RFTKQVVEGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSN 60
           RF KQVVEGRWF+VFASF+IMIGAGS Y+FGTYSK IK+QFDY+QTQ+NTLGF+KDLGSN
Sbjct: 11  RFIKQVVEGRWFAVFASFLIMIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSN 70

Query: 61  LGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQN 120
            GVFAGLL EVAPPWVLF+VGS+LNF+S+ MIWLS+T RI KP FW+MF+++ +AAN+QN
Sbjct: 71  FGVFAGLLGEVAPPWVLFLVGSALNFWSYFMIWLSITGRIVKPPFWEMFVFICVAANSQN 130

Query: 121 FANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLSWFP 180
           FANTA++V SV+NFPDRRGII+GLLKGFVG GGA+LTQ YLA+YGH++P++++LLLSW P
Sbjct: 131 FANTAILVTSVRNFPDRRGIILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLP 190

Query: 181 SVISIVLLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIVFSHAAYLGS 240
           +V+S++   SIR I+ RKHP+EL+V YHLLY SIILA+ +L +T+TQK  VF+  AY+  
Sbjct: 191 TVVSMLFFLSIRPIKVRKHPDELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSG 250

Query: 241 FAVVVALLCLPLLIAIKEEFLLFKLNNQVV--DPSVNLSIAEEK---------ESFPYSN 300
             V++ LL +PLLIAI+EEFLLFKLN Q +  +P+V + + E +         E  P ++
Sbjct: 251 ATVIIGLLLIPLLIAIREEFLLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIEQIPEAS 310

Query: 301 VVVEENSPSKPQRGEDFTILQALLSKDMALIFLATVCACGSSIAAIDNLGQIAESLSYPA 360
                 +  +PQRGEDFTILQAL SKDMAL+F+AT+ ACG+SIAAIDNLGQ+ ESL YP+
Sbjct: 311 TSCFSMAFKRPQRGEDFTILQALFSKDMALVFIATLSACGASIAAIDNLGQVGESLGYPS 370

Query: 361 QSISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHG 420
           Q+IS+FVSW+SIFNFFGRVFSGFIS+ LMTKYKLPRPLMFA +H+LTC+GILFIAFPY G
Sbjct: 371 QTISIFVSWVSIFNFFGRVFSGFISDALMTKYKLPRPLMFAFSHILTCVGILFIAFPYKG 430

Query: 421 SVYVASLIIGFGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYVLNVLLIGRMY 480
           SVY ASLIIGFGFGAQVP+LFAIIS+LFGLK+YST+ NCGQLAVP+GSY+LNV +IGR+Y
Sbjct: 431 SVYAASLIIGFGFGAQVPMLFAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGRLY 490

Query: 481 DMEATRTGNTKNGKGLTCSGAHCFNGSFTILAAITLFGAISSLALAYRTRGFYKGDIYQR 540
           D+EA + G  KNGKGLTC+G  CF+GSF ILA + LFGA++SL LAYRTR FYKGD+Y R
Sbjct: 491 DIEAAKLGGVKNGKGLTCTGTPCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGDVYNR 550

BLAST of MS006868 vs. ExPASy TrEMBL
Match: A0A6J1HIM6 (uncharacterized protein LOC111464777 OS=Cucurbita moschata OX=3662 GN=LOC111464777 PE=4 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 2.7e-220
Identity = 384/550 (69.82%), Postives = 467/550 (84.91%), Query Frame = 0

Query: 1   RFTKQVVEGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSN 60
           RF KQVVEGRWF+VFASF+IMIGAGS Y+FGTYSK IK+QFDY+QTQ+NTLGF+KDLGSN
Sbjct: 11  RFIKQVVEGRWFAVFASFLIMIGAGSTYIFGTYSKAIKSQFDYNQTQINTLGFAKDLGSN 70

Query: 61  LGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQN 120
            GVFAGLL EVAPPWVLF+VGS+LNF+S+ MIWLS+T RI+KP FW+MF+++ +AAN+QN
Sbjct: 71  FGVFAGLLGEVAPPWVLFLVGSALNFWSYFMIWLSITGRISKPPFWEMFVFICVAANSQN 130

Query: 121 FANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLSWFP 180
           FANTA++V SV+NFPDRRGII+GLLKGFVG GGA+LTQ YLA+YGH++P++++LLLSW P
Sbjct: 131 FANTAILVTSVRNFPDRRGIILGLLKGFVGFGGAILTQFYLALYGHDNPTNLVLLLSWLP 190

Query: 181 SVISIVLLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIVFSHAAYLGS 240
           +V S++   SIR I+ RK P+EL+V YHLLY SIILA+ +L +T+TQK  VF+  AY+  
Sbjct: 191 TVASMLFFLSIRPIKVRKDPDELRVLYHLLYVSIILALFLLFLTITQKQTVFTQEAYMSG 250

Query: 241 FAVVVALLCLPLLIAIKEEFLLFKLNNQVV--DPSVNLSIAEEKES--FPYSNVVVEENS 300
             V++ LL +PLLIAI+EEF+LFKLN Q +  +P+V + + E + S   P +   + E S
Sbjct: 251 ATVIIGLLLIPLLIAIREEFMLFKLNKQTLNNNPTVPILMPEHEPSPKAPPTIQQIPEAS 310

Query: 301 PS-------KPQRGEDFTILQALLSKDMALIFLATVCACGSSIAAIDNLGQIAESLSYPA 360
            S       KPQRGEDFTILQAL SKDMAL+F+AT+ ACG+SIAAIDNLGQ+ ESL YP 
Sbjct: 311 TSCFSMTFKKPQRGEDFTILQALFSKDMALVFIATLTACGASIAAIDNLGQVGESLGYPY 370

Query: 361 QSISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHG 420
           Q+IS+FVSW+SIFNFFGRVFSGFISE LMTKYKLPRPLMFA +HLLTCIGILFIAFPY G
Sbjct: 371 QTISIFVSWVSIFNFFGRVFSGFISEALMTKYKLPRPLMFAFSHLLTCIGILFIAFPYKG 430

Query: 421 SVYVASLIIGFGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYVLNVLLIGRMY 480
           SVY ASLIIGFGFGAQVP+LFAIIS+LFGLK+YST+ NCGQLAVP+GSY+LNV +IG++Y
Sbjct: 431 SVYAASLIIGFGFGAQVPMLFAIISELFGLKYYSTIFNCGQLAVPIGSYILNVDVIGKLY 490

Query: 481 DMEATRTGNTKNGKGLTCSGAHCFNGSFTILAAITLFGAISSLALAYRTRGFYKGDIYQR 540
           D+EA + G  KNGKGLTC+G HCF+GSF ILA + LFGA++SL LAYRTR FYKGD+Y R
Sbjct: 491 DIEAAKLGGVKNGKGLTCTGTHCFSGSFLILAVVVLFGAVASLVLAYRTRDFYKGDVYNR 550

BLAST of MS006868 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 552.7 bits (1423), Expect = 3.2e-157
Identity = 290/550 (52.73%), Postives = 385/550 (70.00%), Query Frame = 0

Query: 1   RFTKQVVEGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSN 60
           RF      GRWF VFASF+IM  AG+ YLFGTYSK IK+   Y QT +N LGF KDLG+N
Sbjct: 8   RFLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGAN 67

Query: 61  LGVFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQN 120
           +GV +GL+AEV P W +  +GS++NF  + MIWL+VT ++AKP+ WQM LY+ + AN+QN
Sbjct: 68  VGVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQN 127

Query: 121 FANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLSWFP 180
           FANT  +V  VKNFP+ RG+++GLLKG+VGL GA+ TQ+Y AIYGH D   ++LL++W P
Sbjct: 128 FANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGH-DSKSLILLIAWLP 187

Query: 181 SVISIVLLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIVFSHAAYLGS 240
           + +S+V ++ IR  +  +   EL VFY  LY SI LA+ ++ + + +K + FS AAY  S
Sbjct: 188 AAVSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAAS 247

Query: 241 FAVVVALLCLPLLIAIKEEFLLFKLNNQVVDPSVNLSIAEEKESFPY-----SNVVVEEN 300
             +  ALL +PL +++K+E  ++ +    ++    + + + K+         + V  EE 
Sbjct: 248 ATICCALLFVPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVNGEEK 307

Query: 301 SP--------SKPQRGEDFTILQALLSKDMALIFLATVCACGSSIAAIDNLGQIAESLSY 360
                     S P RGED+TILQALLS DM ++F+AT C  GSS+ A+DNLGQI ESL Y
Sbjct: 308 ETKSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESLGY 367

Query: 361 PAQSISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPY 420
           P  ++S FVS +SI+N+FGRVFSGF+SE L+ KYKLPRPLM  L  LL+C G L IAFP 
Sbjct: 368 PNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIAFPV 427

Query: 421 HGSVYVASLIIGFGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYVLNVLLIGR 480
            GSVY+AS+++GF FGAQ+PLLFAIIS+LFGLK+YSTL NCGQLA PLGSY+LNV + G 
Sbjct: 428 PGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRVTGM 487

Query: 481 MYDMEA----TRTGNT-KNGKGLTCSGAHCFNGSFTILAAITLFGAISSLALAYRTRGFY 533
           +YD EA    T  G T K+ K LTC G+ C+   F ILAA+T FGA+ SL LA RTR FY
Sbjct: 488 LYDKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRTREFY 547

BLAST of MS006868 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 508.1 bits (1307), Expect = 8.9e-144
Identity = 265/553 (47.92%), Postives = 371/553 (67.09%), Query Frame = 0

Query: 3   TKQVVEGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSNLG 62
           T Q++ GRWF  F S +IM  AG+ Y+FG YS  IK    Y QT +N L F KDLG+N+G
Sbjct: 14  TIQILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVG 73

Query: 63  VFAGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQNFA 122
           V AGLL EV PPW + ++G+ LNFF + MIWL+VT RI+KP+ W M LY+ + AN+Q+FA
Sbjct: 74  VLAGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFA 133

Query: 123 NTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLSWFPSV 182
           NT  +V  VKNFP+ RG+++G+LKG+VGL GA++TQ+Y A YG ED  +++L++ W P++
Sbjct: 134 NTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYG-EDTKELILMIGWLPAI 193

Query: 183 ISIVLLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQKHIVFSHAAYLGSFA 242
           +S   L +IR ++ ++   ELKVFY+ LY S+ LA  ++++ +  K   F+ + + GS A
Sbjct: 194 VSFAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAA 253

Query: 243 VVVALLCLPLLIAIKEEFLLFKLNNQVVDPSVNLSIAEEKESFPYSNVVVEENSPSK--- 302
           VV+ LL LP+++ I EE  L+K     ++    +++  EK     S    ++   SK   
Sbjct: 254 VVIVLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEKPKLDSSEFKDDDGEESKEVV 313

Query: 303 ---------------PQRGEDFTILQALLSKDMALIFLATVCACGSSIAAIDNLGQIAES 362
                          P+RG+D+TILQAL S DM ++FLAT+C  G ++ AIDNLGQI  S
Sbjct: 314 EKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIGNS 373

Query: 363 LSYPAQSISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIA 422
           L YP +S+S FVS +SI+N++GRV SG +SE  + KYK PRPLM  +  LL+C G L IA
Sbjct: 374 LGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIA 433

Query: 423 FPYHGSVYVASLIIGFGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYVLNVLL 482
           F   G +YVAS+IIGF FGAQ PLLFAIIS++FGLK+YSTL N G +A P+GSY+LNV +
Sbjct: 434 FNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLNVRV 493

Query: 483 IGRMYDMEATR----TGNTK-NGKGLTCSGAHCFNGSFTILAAITLFGAISSLALAYRTR 533
            G +YD+EA +     G T+  G+ L C G  CF  SF I+AA+TLFG + S+ L  RT+
Sbjct: 494 AGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVIRTK 553

BLAST of MS006868 vs. TAIR 10
Match: AT5G50630.1 (Major facilitator superfamily protein )

HSP 1 Score: 297.0 bits (759), Expect = 3.1e-80
Identity = 180/527 (34.16%), Postives = 299/527 (56.74%), Query Frame = 0

Query: 6   VVEGRWFSVFASFVIMIGAGSPYLF-GTYSKVIKTQFDYSQTQVNTLGFSKDLGSNLGVF 65
           +V  RW     +  +   AG  YLF G+ S  IKT   Y+Q Q+  LG +K+LG  +G  
Sbjct: 12  LVNDRWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGDAIGFV 71

Query: 66  AGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQNFANT 125
           +G L+EV+P WV+ +VG++ N F + ++WL VT ++     W +F+ + +  N + + NT
Sbjct: 72  SGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETYYNT 131

Query: 126 AVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLSWFPSVIS 185
           A +V  + NFP+ RG ++G+LKGF GL GA+LTQ+YL ++     S V+L+++  P V+ 
Sbjct: 132 ASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYL-MFNPSHDSSVILMVALGPPVVV 191

Query: 186 IVLLFSIRTI----RARKHPEELKVFYHLLYASIILAISILIITLTQKHIVFSHAAYLGS 245
           + LLF +R +    R     ++L+ F  +    ++LA+ +L + + Q     +      S
Sbjct: 192 LALLFIVRPVERSCRTNLRSDDLR-FLAIYGFCVVLAVYLLGLLVLQSVFDMTQTIITTS 251

Query: 246 FAVVVALLCLPLLIAIKEEFLLFKLNNQVVDPSVNLSIAEEKESFPYSNVVVEENSPSKP 305
            A++V  + +P+L+     F+    N   V P    S  ++ E+     ++   + P + 
Sbjct: 252 GAILVIFMVVPVLVPFSSVFISGN-NVTSVKPEEGTSNVDQHEA---RTLIERSDRPPEK 311

Query: 306 QR----GEDFTILQALLSKDMALIFLATVCACGSSIAAIDNLGQIAESLSYPAQSISVFV 365
           +R    GEDFT+LQAL   D  LIF++ V   GS I  IDNLGQI  SL Y   +  +FV
Sbjct: 312 KRAPCIGEDFTLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYSLGY--SNTKIFV 371

Query: 366 SWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHGSVYVASL 425
           S ISI NF GRV  G+ SE ++ K  LPR L  ++   +  +G+++ A  + G +YV ++
Sbjct: 372 SLISISNFLGRVAGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYYAIDWPGKIYVVTI 431

Query: 426 IIGFGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYVLNVLLIGRMYDMEATR- 485
           +IG G+GA   +  A +SD+FGLK + +L N    A+P+GS+V + ++   +YD  A + 
Sbjct: 432 VIGMGYGAHWAIAPASVSDIFGLKSFGSLYNFQITALPIGSFVFSGVIASNIYDYYARKQ 491

Query: 486 TGNTKNGKGLTCSGAHCFNGSFTILAAITLFGAISSLALAYRTRGFY 523
            G T   + L C+G+ C++ + ++++ + L   + SL++ YRTR FY
Sbjct: 492 AGPTTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFY 530

BLAST of MS006868 vs. TAIR 10
Match: AT5G50520.1 (Major facilitator superfamily protein )

HSP 1 Score: 297.0 bits (759), Expect = 3.1e-80
Identity = 180/527 (34.16%), Postives = 299/527 (56.74%), Query Frame = 0

Query: 6   VVEGRWFSVFASFVIMIGAGSPYLF-GTYSKVIKTQFDYSQTQVNTLGFSKDLGSNLGVF 65
           +V  RW     +  +   AG  YLF G+ S  IKT   Y+Q Q+  LG +K+LG  +G  
Sbjct: 12  LVNDRWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGDAIGFV 71

Query: 66  AGLLAEVAPPWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQMFLYVGLAANAQNFANT 125
           +G L+EV+P WV+ +VG++ N F + ++WL VT ++     W +F+ + +  N + + NT
Sbjct: 72  SGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETYYNT 131

Query: 126 AVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHEDPSDVLLLLSWFPSVIS 185
           A +V  + NFP+ RG ++G+LKGF GL GA+LTQ+YL ++     S V+L+++  P V+ 
Sbjct: 132 ASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYL-MFNPSHDSSVILMVALGPPVVV 191

Query: 186 IVLLFSIRTI----RARKHPEELKVFYHLLYASIILAISILIITLTQKHIVFSHAAYLGS 245
           + LLF +R +    R     ++L+ F  +    ++LA+ +L + + Q     +      S
Sbjct: 192 LALLFIVRPVERSCRTNLRSDDLR-FLAIYGFCVVLAVYLLGLLVLQSVFDMTQTIITTS 251

Query: 246 FAVVVALLCLPLLIAIKEEFLLFKLNNQVVDPSVNLSIAEEKESFPYSNVVVEENSPSKP 305
            A++V  + +P+L+     F+    N   V P    S  ++ E+     ++   + P + 
Sbjct: 252 GAILVIFMVVPVLVPFSSVFISGN-NVTSVKPEEGTSNVDQHEA---RTLIERSDRPPEK 311

Query: 306 QR----GEDFTILQALLSKDMALIFLATVCACGSSIAAIDNLGQIAESLSYPAQSISVFV 365
           +R    GEDFT+LQAL   D  LIF++ V   GS I  IDNLGQI  SL Y   +  +FV
Sbjct: 312 KRAPCIGEDFTLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYSLGY--SNTKIFV 371

Query: 366 SWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYHGSVYVASL 425
           S ISI NF GRV  G+ SE ++ K  LPR L  ++   +  +G+++ A  + G +YV ++
Sbjct: 372 SLISISNFLGRVAGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYYAIDWPGKIYVVTI 431

Query: 426 IIGFGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYVLNVLLIGRMYDMEATR- 485
           +IG G+GA   +  A +SD+FGLK + +L N    A+P+GS+V + ++   +YD  A + 
Sbjct: 432 VIGMGYGAHWAIAPASVSDIFGLKSFGSLYNFQITALPIGSFVFSGVIASNIYDYYARKQ 491

Query: 486 TGNTKNGKGLTCSGAHCFNGSFTILAAITLFGAISSLALAYRTRGFY 523
            G T   + L C+G+ C++ + ++++ + L   + SL++ YRTR FY
Sbjct: 492 AGPTTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFY 530

BLAST of MS006868 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 288.5 bits (737), Expect = 1.1e-77
Identity = 187/541 (34.57%), Postives = 301/541 (55.64%), Query Frame = 0

Query: 5   QVVEGRWFSVFASFVIMIGAGSPYLFGTYSKVIKTQFDYSQTQVNTLGFSKDLGSNLGVF 64
           +++  +W ++ AS  I   +G+ Y FG YS V+K+   Y Q+ ++T+   KD+G+N GVF
Sbjct: 2   EILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVF 61

Query: 65  AGLLAEVAP----------------PWVLFVVGSSLNFFSFIMIWLSVTHRIAKPEFWQM 124
           +GLL   A                 PWV+  VG+   F  + +IW SVT  I KP    M
Sbjct: 62  SGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLM 121

Query: 125 FLYVGLAANAQNFANTAVMVISVKNFPDRRGIIIGLLKGFVGLGGAVLTQIYLAIYGHED 184
            L++ LAA +Q F NTA +V +V+NF D  G  +G++KGF+GL GA+L Q+Y  +    D
Sbjct: 122 CLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCA-GD 181

Query: 185 PSDVLLLLSWFPSVISIVLLFSIRTIRARKHPEELKVFYHLLYASIILAISILIITLTQK 244
           P+  +LLL+  P+V+S++++  +R I      ++ K    L   S+I+A  ++II + + 
Sbjct: 182 PASFILLLAVTPTVLSLLVMPLVR-IYETSVADDKKHLNGLSAVSLIIAAYLMIIIILKN 241

Query: 245 HIVFSHAAYLGSFAVVVALLCLPLLIAIKEEFLLFKLNNQVVDPSVNLSIAEEKESFPYS 304
               S  A + +   ++ +L LPLLIA + +    +   +   P     +    ++    
Sbjct: 242 TFGLSSWANIVTLVCLLVMLALPLLIARRAQ----RDGMEKTVPHDYSPLISSPKATTSG 301

Query: 305 NVVVEENSPSKPQRGEDFTILQALLSKDMALIFLATVCACGSSIAAIDNLGQIAESLSYP 364
           N   E +S  +    E+  +LQA+      L+FLA +C  GS ++ I+N+ QI ESL Y 
Sbjct: 302 NQSSEGDSKVEAGLSENLNLLQAMKKLSFWLLFLAMICGMGSGLSTINNIRQIGESLRYS 361

Query: 365 AQSISVFVSWISIFNFFGRVFSGFISETLMTKYKLPRPLMFALAHLLTCIGILFIAFPYH 424
           +  I+  VS  SI+NF GR  +G+ S+ L+ K   PRPL+ A       IG L IA  + 
Sbjct: 362 SVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSIGHLIIASGFQ 421

Query: 425 GSVYVASLIIGFGFGAQVPLLFAIISDLFGLKHYSTLLNCGQLAVPLGSYVLNVLLIGRM 484
           G++YV S+I+G  +G+Q  L+  I S+LFG++H  T+ N   +A P+GSY+ +V LIG +
Sbjct: 422 GNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVASPIGSYIFSVRLIGYI 481

Query: 485 YDMEATRTGNTKNGKGLTCSGAHCFNGSFTILAAITLFGAISSLALAYRTRGFYKGDIYQ 530
           YD        T +G+G TC G+HCF  SF I+A++  FG + ++ L +RT+  Y+  + +
Sbjct: 482 YD-------KTASGEGNTCYGSHCFRLSFIIMASVAFFGFLVAIVLFFRTKTLYRQILVK 529

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152848.11.2e-28093.19uncharacterized membrane protein YMR155W-like [Momordica charantia][more]
XP_022152849.18.7e-22674.54uncharacterized protein LOC111020473 [Momordica charantia][more]
XP_022152845.13.1e-22371.14uncharacterized protein LOC111020469 [Momordica charantia][more]
XP_038895745.18.5e-22171.96uncharacterized protein LOC120083909 [Benincasa hispida][more]
KAG6583549.11.9e-22070.00Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
F4I9E13.0e-2724.63Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Q037954.1e-0822.91Uncharacterized membrane protein YMR155W OS=Saccharomyces cerevisiae (strain ATC... [more]
Match NameE-valueIdentityDescription
A0A6J1DG006.0e-28193.19uncharacterized membrane protein YMR155W-like OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1DHB04.2e-22674.54uncharacterized protein LOC111020473 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
A0A6J1DF431.5e-22371.14uncharacterized protein LOC111020469 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
A0A6J1HYK51.6e-22068.91uncharacterized protein LOC111469251 OS=Cucurbita maxima OX=3661 GN=LOC111469251... [more]
A0A6J1HIM62.7e-22069.82uncharacterized protein LOC111464777 OS=Cucurbita moschata OX=3662 GN=LOC1114647... [more]
Match NameE-valueIdentityDescription
AT2G28120.13.2e-15752.73Major facilitator superfamily protein [more]
AT2G39210.18.9e-14447.92Major facilitator superfamily protein [more]
AT5G50630.13.1e-8034.16Major facilitator superfamily protein [more]
AT5G50520.13.1e-8034.16Major facilitator superfamily protein [more]
AT1G74780.11.1e-7734.57Nodulin-like / Major Facilitator Superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 10..256
e-value: 6.2E-75
score: 251.9
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 7..204
e-value: 2.9E-7
score: 32.1
coord: 316..521
e-value: 8.0E-9
score: 37.2
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 11..521
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 1..533
NoneNo IPR availablePANTHERPTHR21576:SF29PROTEIN NUCLEAR FUSION DEFECTIVE 4coord: 1..533
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 9..508
e-value: 2.30946E-94
score: 291.077

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS006868.1MS006868.1mRNA