MS006835 (gene) Bitter gourd (TR) v1

Overview
NameMS006835
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionXyloglucan endotransglucosylase/hydrolase
Locationscaffold60: 1075123 .. 1076429 (+)
RNA-Seq ExpressionMS006835
SyntenyMS006835
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAATGTCTCGTCTTCTTGTTCTTCTCTCTCTCCCAATTCTCTTCCTCTCTCTAAGCTCTTGCCAGAACCCCCCCTCCCCTGGCTACCACCCAAGCAACCAAATTCAGACCATTGGGTTCGATCAGGGCTTTAGAAATCTCTGGGGCTCTCAGCACCAGAGACTTGACCACGGCGCGTTAACCATTTGGCTTGACGCTACGTCGGGTATACTAGATAAAACTTTAAAATCTTTACTTTTTAATATGATTTTTTTTCTTTCTTTAAATTTTAAGTGAACTCACTTTCTTATGAACCTGATAAAAAAATTTGGTTGGTAAAACAGGTAGTGGGTTCAAATCCCTCCGTCGATACCAATCTGGCTACTTTGGAGCTGCCATTAAGCTTCATCCTGGCTACACTGCTGGAGTTATCACTTCTCTTTATGTAAGCAGTACTATATATATGTATATATTCGATTTTGATATGAATAAATAATTAAATTTACTGTCTTCGTATCAGAATAGGAGATTATATGTTTAAAGTTCAACGACGTGTTGCGATTAAGTTTGGTAGATGGGTTAATTTTTAAGTTCATTAGTGAGAGTTTAGCGGTGATTTAATTGTTTTTTCTCTTCTTTATTTGGGTTTTGTAGCTTTCGAACAATGAAGATTTCCCGGGGTACCATGACGAGATCGATATCGAGTTCCTCGGGACGACGTCAGACAAGCCATATATTCTGCAGACAAATGTGTACATGAGAGGGAGTGGAGATGGAAATATCATAGGAAGGGAAATGAGGTTCCATCTTTGGTTTGACCCAACTCAAGATTTCCACAACTACGCCATTTCGTGGACTCCAAGAGAAATTATGTAAGCTTACGAAGTTAAACTAAATTGTTACTTCAATAAAAATACAGAGACTAAATATAATTTTTTTAACTCGTTCGTTTTGTCTATATGTTGTATAGATTCTTCGTGGACGACGTACCAATAAGGAGGTATGAGAGGAAGAGTGACGCGACGTTTCCAGTGAGGCCAATGTGGGTGTACGGGTCAATCTGGGACGCGTCGTCGTGGGCGACGGAGGATGGAAAGTACAAGGCTGATTACAAATACCAACCGTTCATCGGAAGGTACAACAACTTCAAGCTGGGCGGGTGCACGGTGGACGGGGCAGCATCGTGTCGTCCACCGAGCACCGGAGCGAGCGGCGGGGAGGGGCTGAGCCAGCAGCAGGAGAAGGCAATGGAGTGGGTGCAGAACAATTACTTGGTGTACAACTACTGCCATGATCCCAAGAGAGATCATACCTTAATTCCTGAGTGC

mRNA sequence

AAAATGTCTCGTCTTCTTGTTCTTCTCTCTCTCCCAATTCTCTTCCTCTCTCTAAGCTCTTGCCAGAACCCCCCCTCCCCTGGCTACCACCCAAGCAACCAAATTCAGACCATTGGGTTCGATCAGGGCTTTAGAAATCTCTGGGGCTCTCAGCACCAGAGACTTGACCACGGCGCGTTAACCATTTGGCTTGACGCTACGTCGGGTAGTGGGTTCAAATCCCTCCGTCGATACCAATCTGGCTACTTTGGAGCTGCCATTAAGCTTCATCCTGGCTACACTGCTGGAGTTATCACTTCTCTTTATCTTTCGAACAATGAAGATTTCCCGGGGTACCATGACGAGATCGATATCGAGTTCCTCGGGACGACGTCAGACAAGCCATATATTCTGCAGACAAATGTGTACATGAGAGGGAGTGGAGATGGAAATATCATAGGAAGGGAAATGAGGTTCCATCTTTGGTTTGACCCAACTCAAGATTTCCACAACTACGCCATTTCGTGGACTCCAAGAGAAATTATATTCTTCGTGGACGACGTACCAATAAGGAGGTATGAGAGGAAGAGTGACGCGACGTTTCCAGTGAGGCCAATGTGGGTGTACGGGTCAATCTGGGACGCGTCGTCGTGGGCGACGGAGGATGGAAAGTACAAGGCTGATTACAAATACCAACCGTTCATCGGAAGGTACAACAACTTCAAGCTGGGCGGGTGCACGGTGGACGGGGCAGCATCGTGTCGTCCACCGAGCACCGGAGCGAGCGGCGGGGAGGGGCTGAGCCAGCAGCAGGAGAAGGCAATGGAGTGGGTGCAGAACAATTACTTGGTGTACAACTACTGCCATGATCCCAAGAGAGATCATACCTTAATTCCTGAGTGC

Coding sequence (CDS)

AAAATGTCTCGTCTTCTTGTTCTTCTCTCTCTCCCAATTCTCTTCCTCTCTCTAAGCTCTTGCCAGAACCCCCCCTCCCCTGGCTACCACCCAAGCAACCAAATTCAGACCATTGGGTTCGATCAGGGCTTTAGAAATCTCTGGGGCTCTCAGCACCAGAGACTTGACCACGGCGCGTTAACCATTTGGCTTGACGCTACGTCGGGTAGTGGGTTCAAATCCCTCCGTCGATACCAATCTGGCTACTTTGGAGCTGCCATTAAGCTTCATCCTGGCTACACTGCTGGAGTTATCACTTCTCTTTATCTTTCGAACAATGAAGATTTCCCGGGGTACCATGACGAGATCGATATCGAGTTCCTCGGGACGACGTCAGACAAGCCATATATTCTGCAGACAAATGTGTACATGAGAGGGAGTGGAGATGGAAATATCATAGGAAGGGAAATGAGGTTCCATCTTTGGTTTGACCCAACTCAAGATTTCCACAACTACGCCATTTCGTGGACTCCAAGAGAAATTATATTCTTCGTGGACGACGTACCAATAAGGAGGTATGAGAGGAAGAGTGACGCGACGTTTCCAGTGAGGCCAATGTGGGTGTACGGGTCAATCTGGGACGCGTCGTCGTGGGCGACGGAGGATGGAAAGTACAAGGCTGATTACAAATACCAACCGTTCATCGGAAGGTACAACAACTTCAAGCTGGGCGGGTGCACGGTGGACGGGGCAGCATCGTGTCGTCCACCGAGCACCGGAGCGAGCGGCGGGGAGGGGCTGAGCCAGCAGCAGGAGAAGGCAATGGAGTGGGTGCAGAACAATTACTTGGTGTACAACTACTGCCATGATCCCAAGAGAGATCATACCTTAATTCCTGAGTGC

Protein sequence

KMSRLLVLLSLPILFLSLSSCQNPPSPGYHPSNQIQTIGFDQGFRNLWGSQHQRLDHGALTIWLDATSGSGFKSLRRYQSGYFGAAIKLHPGYTAGVITSLYLSNNEDFPGYHDEIDIEFLGTTSDKPYILQTNVYMRGSGDGNIIGREMRFHLWFDPTQDFHNYAISWTPREIIFFVDDVPIRRYERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCTVDGAASCRPPSTGASGGEGLSQQQEKAMEWVQNNYLVYNYCHDPKRDHTLIPEC
Homology
BLAST of MS006835 vs. NCBI nr
Match: XP_038894127.1 (probable xyloglucan endotransglucosylase/hydrolase protein 32 [Benincasa hispida] >XP_038894129.1 probable xyloglucan endotransglucosylase/hydrolase protein 32 [Benincasa hispida])

HSP 1 Score: 543.9 bits (1400), Expect = 8.6e-151
Identity = 252/294 (85.71%), Postives = 267/294 (90.82%), Query Frame = 0

Query: 3   SRLLVLLSLPILFLSLSSCQNPPSPGYHPSNQIQTIGFDQGFRNLWGSQHQRLDHGALTI 62
           S  L+LLSL   F S +S QNPPSPGY PSNQ+Q+IGFDQGFRN WGSQHQ++D G LTI
Sbjct: 4   SYFLILLSL-FFFFSFTSSQNPPSPGYFPSNQVQSIGFDQGFRNRWGSQHQKVDQGTLTI 63

Query: 63  WLDATSGSGFKSLRRYQSGYFGAAIKLHPGYTAGVITSLYLSNNEDFPGYHDEIDIEFLG 122
           WLD++SGSGFKSL RYQSGYFGAAIKLHPGYTAGVITS YLSNNED+PG HDEIDIEFLG
Sbjct: 64  WLDSSSGSGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGNHDEIDIEFLG 123

Query: 123 TTSDKPYILQTNVYMRGSGDGNIIGREMRFHLWFDPTQDFHNYAISWTPREIIFFVDDVP 182
           TT DKPYILQTNV+MRGSGDGNIIGREMRFHLWFDPTQDFHNYAI WTP EIIF VDDVP
Sbjct: 124 TTFDKPYILQTNVFMRGSGDGNIIGREMRFHLWFDPTQDFHNYAILWTPEEIIFLVDDVP 183

Query: 183 IRRYERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCTVD 242
           IRRYERKSDATFPV+PMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGC  D
Sbjct: 184 IRRYERKSDATFPVKPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCATD 243

Query: 243 GAASCRPPSTGASGG--EGLSQQQEKAMEWVQNNYLVYNYCHDPKRDHTLIPEC 295
           GAASCRP ++G SGG   GLSQQQEKAMEWVQ+NYLVYNYCHDP+RDHTL PEC
Sbjct: 244 GAASCRPLNSGPSGGGRGGLSQQQEKAMEWVQSNYLVYNYCHDPRRDHTLTPEC 296

BLAST of MS006835 vs. NCBI nr
Match: XP_023519902.1 (probable xyloglucan endotransglucosylase/hydrolase protein 32 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 526.6 bits (1355), Expect = 1.4e-145
Identity = 241/289 (83.39%), Postives = 260/289 (89.97%), Query Frame = 0

Query: 6   LVLLSLPILFLSLSSCQNPPSPGYHPSNQIQTIGFDQGFRNLWGSQHQRLDHGALTIWLD 65
           L+LLSL  LF S  S QNPPSPGY PSNQ+Q+I FD GFRN WGSQHQ++D G LTIWLD
Sbjct: 6   LILLSL-FLFFSFISSQNPPSPGYFPSNQVQSIAFDHGFRNRWGSQHQKVDQGTLTIWLD 65

Query: 66  ATSGSGFKSLRRYQSGYFGAAIKLHPGYTAGVITSLYLSNNEDFPGYHDEIDIEFLGTTS 125
           ++SGSGFKSL RYQSGYFGAAIKLHPGYTAGVITS YLSNNED+PG HDEIDIEFLGTTS
Sbjct: 66  SSSGSGFKSLNRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGNHDEIDIEFLGTTS 125

Query: 126 DKPYILQTNVYMRGSGDGNIIGREMRFHLWFDPTQDFHNYAISWTPREIIFFVDDVPIRR 185
           DKPY+LQTNV+MRGSGDGNIIGREMRFHLWFDPTQDFHNYAI WT  +IIF VDDVPIRR
Sbjct: 126 DKPYVLQTNVFMRGSGDGNIIGREMRFHLWFDPTQDFHNYAILWTREQIIFLVDDVPIRR 185

Query: 186 YERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCTVDGAA 245
           YE+K++AT+PVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGC  DGAA
Sbjct: 186 YEKKTEATYPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCAADGAA 245

Query: 246 SCRPPSTGASGGEGLSQQQEKAMEWVQNNYLVYNYCHDPKRDHTLIPEC 295
           SC P S G   G G+SQQQE+AMEWVQ+NYLVYNYCHDP+RDHTL PEC
Sbjct: 246 SCHPLSGGP--GSGMSQQQERAMEWVQSNYLVYNYCHDPRRDHTLTPEC 291

BLAST of MS006835 vs. NCBI nr
Match: XP_022949180.1 (xyloglucan endotransglucosylase/hydrolase protein 31-like [Cucurbita moschata] >KAG6607155.1 putative xyloglucan endotransglucosylase/hydrolase protein 32, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 525.0 bits (1351), Expect = 4.1e-145
Identity = 240/293 (81.91%), Postives = 262/293 (89.42%), Query Frame = 0

Query: 2   MSRLLVLLSLPILFLSLSSCQNPPSPGYHPSNQIQTIGFDQGFRNLWGSQHQRLDHGALT 61
           M+  LVLL    +F S SS  NPPSPG++PSNQ+Q+IGFD GFRN WGSQHQ++DHGALT
Sbjct: 1   MAHFLVLL---FVFFSCSSALNPPSPGFYPSNQVQSIGFDHGFRNRWGSQHQKVDHGALT 60

Query: 62  IWLDATSGSGFKSLRRYQSGYFGAAIKLHPGYTAGVITSLYLSNNEDFPGYHDEIDIEFL 121
           IWLD++SGSGFKSL RY+SGYFGAAIKLHPGYTAGVITS YLSNNED+PG HDEIDIEFL
Sbjct: 61  IWLDSSSGSGFKSLHRYKSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGNHDEIDIEFL 120

Query: 122 GTTSDKPYILQTNVYMRGSGDGNIIGREMRFHLWFDPTQDFHNYAISWTPREIIFFVDDV 181
           GTT  KPY+LQTNV+MRGSGDGNIIGREMRFHLWFDPTQDFHNYAI WTP EIIF VDDV
Sbjct: 121 GTTPGKPYVLQTNVFMRGSGDGNIIGREMRFHLWFDPTQDFHNYAILWTPEEIIFLVDDV 180

Query: 182 PIRRYERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCTV 241
           PIRRY +KS+ATFP+RPMWVYGSIWDASSWATEDGKYKADY+YQPFIGRYNNFKLGGC V
Sbjct: 181 PIRRYVKKSEATFPIRPMWVYGSIWDASSWATEDGKYKADYRYQPFIGRYNNFKLGGCAV 240

Query: 242 DGAASCRPPSTGASGGEGLSQQQEKAMEWVQNNYLVYNYCHDPKRDHTLIPEC 295
           DGAA+CR  S+G  GG GLSQQQEKAM WVQ NYL+YNYCHDP+RDHT  PEC
Sbjct: 241 DGAATCRRVSSG-PGGRGLSQQQEKAMAWVQGNYLMYNYCHDPRRDHTSTPEC 289

BLAST of MS006835 vs. NCBI nr
Match: KAG6583568.1 (putative xyloglucan endotransglucosylase/hydrolase protein 32, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 524.2 bits (1349), Expect = 7.0e-145
Identity = 239/289 (82.70%), Postives = 260/289 (89.97%), Query Frame = 0

Query: 6   LVLLSLPILFLSLSSCQNPPSPGYHPSNQIQTIGFDQGFRNLWGSQHQRLDHGALTIWLD 65
           L+LLSL  LF S  S QNPPSPGY PSNQ+Q+I FD GFRN WGS+HQ++D G LTIWLD
Sbjct: 6   LILLSL-FLFFSFISSQNPPSPGYFPSNQVQSIAFDHGFRNRWGSEHQKVDQGTLTIWLD 65

Query: 66  ATSGSGFKSLRRYQSGYFGAAIKLHPGYTAGVITSLYLSNNEDFPGYHDEIDIEFLGTTS 125
           ++SGSGFKSL RYQSGYFGAAIKLHPGYTAGVITS YLSNNED+PG HDEIDIEFLGTTS
Sbjct: 66  SSSGSGFKSLNRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGNHDEIDIEFLGTTS 125

Query: 126 DKPYILQTNVYMRGSGDGNIIGREMRFHLWFDPTQDFHNYAISWTPREIIFFVDDVPIRR 185
           DKPY+LQTNV+MRGSGDGNIIGREMRFHLWFDPTQDFHNYAI WT  +IIF VDDVPIRR
Sbjct: 126 DKPYVLQTNVFMRGSGDGNIIGREMRFHLWFDPTQDFHNYAILWTREQIIFLVDDVPIRR 185

Query: 186 YERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCTVDGAA 245
           YE+K++AT+PVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGC  DGA+
Sbjct: 186 YEKKTEATYPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCAADGAS 245

Query: 246 SCRPPSTGASGGEGLSQQQEKAMEWVQNNYLVYNYCHDPKRDHTLIPEC 295
           SC P S G   G G+SQQQE+AMEWVQ+NYLVYNYCHDP+RDHTL PEC
Sbjct: 246 SCHPLSGGP--GSGMSQQQERAMEWVQSNYLVYNYCHDPRRDHTLTPEC 291

BLAST of MS006835 vs. NCBI nr
Match: XP_004147295.2 (xyloglucan endotransglucosylase/hydrolase protein 31 [Cucumis sativus] >KGN64814.1 hypothetical protein Csa_013724 [Cucumis sativus])

HSP 1 Score: 523.5 bits (1347), Expect = 1.2e-144
Identity = 245/293 (83.62%), Postives = 261/293 (89.08%), Query Frame = 0

Query: 6   LVLLSL-PILFLSLSSCQN-PPSPGYHPSNQIQTIGFDQGFRNLWGSQHQRLDHGALTIW 65
           L LLSL    F S +S QN PPSPGY PS Q+Q+ GFDQ FRN WGSQHQ++D G LTIW
Sbjct: 7   LFLLSLFFFFFFSFTSSQNYPPSPGYFPSTQVQSTGFDQVFRNRWGSQHQKVDQGTLTIW 66

Query: 66  LDATSGSGFKSLRRYQSGYFGAAIKLHPGYTAGVITSLYLSNNEDFPGYHDEIDIEFLGT 125
           LD++SGSGFKSL RYQSGYFGAAIKLHPGYTAGVITS YLSNNED+PG HDEIDIEFLGT
Sbjct: 67  LDSSSGSGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGNHDEIDIEFLGT 126

Query: 126 TSDKPYILQTNVYMRGSGDGNIIGREMRFHLWFDPTQDFHNYAISWTPREIIFFVDDVPI 185
           TSDKPY+LQTNV+MRGSGDGNIIGREMRFHLWF+PTQDFHNYAI WTP EIIF VDDVPI
Sbjct: 127 TSDKPYVLQTNVFMRGSGDGNIIGREMRFHLWFNPTQDFHNYAILWTPEEIIFLVDDVPI 186

Query: 186 RRYERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCTVDG 245
           RRYERKS+ATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKL GCT DG
Sbjct: 187 RRYERKSEATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLSGCTTDG 246

Query: 246 AASCRPPSTGASGG--EGLSQQQEKAMEWVQNNYLVYNYCHDPKRDHTLIPEC 295
           AASCRP ++G  GG    +SQQQEKAMEWVQNNYLVYNYCHDP+RDHTL PEC
Sbjct: 247 AASCRPLNSGPGGGGRGRMSQQQEKAMEWVQNNYLVYNYCHDPRRDHTLTPEC 299

BLAST of MS006835 vs. ExPASy Swiss-Prot
Match: P93046 (Xyloglucan endotransglucosylase/hydrolase protein 31 OS=Arabidopsis thaliana OX=3702 GN=XTH31 PE=1 SV=2)

HSP 1 Score: 436.8 bits (1122), Expect = 1.9e-121
Identity = 194/293 (66.21%), Postives = 233/293 (79.52%), Query Frame = 0

Query: 2   MSRLLVLLSLPILFLSLSSCQNPPSPGYHPSNQIQTIGFDQGFRNLWGSQHQRLDHGALT 61
           M+  L+ L+L +L  S    Q  PSPGY+PS+++ T  FD+ FR LWGSQHQR +   +T
Sbjct: 1   MALSLIFLALLVLCPSSGHSQRSPSPGYYPSSRVPTSPFDREFRTLWGSQHQRREQDVVT 60

Query: 62  IWLDATSGSGFKSLRRYQSGYFGAAIKLHPGYTAGVITSLYLSNNEDFPGYHDEIDIEFL 121
           +WLD ++GSGFKSLR Y+SGYFGA+IKL PG+TAGV TSLYLSNN++ PG HDE+DIEFL
Sbjct: 61  LWLDKSTGSGFKSLRPYRSGYFGASIKLQPGFTAGVDTSLYLSNNQEHPGDHDEVDIEFL 120

Query: 122 GTTSDKPYILQTNVYMRGSGDGNIIGREMRFHLWFDPTQDFHNYAISWTPREIIFFVDDV 181
           GTT  KPY LQTNV++RGSGD N+IGREM+F LWFDPTQDFH+YAI W P +I+FFVDDV
Sbjct: 121 GTTPGKPYSLQTNVFVRGSGDRNVIGREMKFTLWFDPTQDFHHYAILWNPNQIVFFVDDV 180

Query: 182 PIRRYERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCTV 241
           PIR Y RK++A FP RPMWVYGSIWDAS WATE+G+ KADY+YQPF+ +Y NFKL GCT 
Sbjct: 181 PIRTYNRKNEAIFPTRPMWVYGSIWDASDWATENGRIKADYRYQPFVAKYKNFKLAGCTA 240

Query: 242 DGAASCRPPSTGASGGEGLSQQQEKAMEWVQNNYLVYNYCHDPKRDHTLIPEC 295
           D ++SCRPPS       GLS+QQ  A+ W Q N+LVYNYCHDPKRDHT  PEC
Sbjct: 241 DSSSSCRPPSPAPMRNRGLSRQQMAALTWAQRNFLVYNYCHDPKRDHTQTPEC 293

BLAST of MS006835 vs. ExPASy Swiss-Prot
Match: Q9SJL9 (Probable xyloglucan endotransglucosylase/hydrolase protein 32 OS=Arabidopsis thaliana OX=3702 GN=XTH32 PE=2 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 2.8e-120
Identity = 198/290 (68.28%), Postives = 233/290 (80.34%), Query Frame = 0

Query: 5   LLVLLSLPILFLSLSSCQNPPSPGYHPSNQIQTIGFDQGFRNLWGSQHQRLDHGALTIWL 64
           LL +  L +L+ S  +   PPSPGY PS+++ ++ F +GFRNLWG QHQR+D  ALTIWL
Sbjct: 8   LLSIFHLLVLWGSSVNAYWPPSPGYWPSSKVGSLNFYKGFRNLWGPQHQRMDQNALTIWL 67

Query: 65  DATSGSGFKSLRRYQSGYFGAAIKLHPGYTAGVITSLYLSNNEDFPGYHDEIDIEFLGTT 124
           D TSGSGFKS++ ++SGYFGA IKL PGYTAGVITSLYLSNNE  PG+HDE+DIEFLGTT
Sbjct: 68  DRTSGSGFKSVKPFRSGYFGANIKLQPGYTAGVITSLYLSNNEAHPGFHDEVDIEFLGTT 127

Query: 125 SDKPYILQTNVYMRGSGDGNIIGREMRFHLWFDPTQDFHNYAISWTPREIIFFVDDVPIR 184
             KPY LQTNVY+RGSGDG IIGREM+F LWFDPT+DFH+YAI W+PREIIF VDD+PIR
Sbjct: 128 FGKPYTLQTNVYIRGSGDGKIIGREMKFRLWFDPTKDFHHYAILWSPREIIFLVDDIPIR 187

Query: 185 RYERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCTVDGA 244
           RY +KS +TFP+RPMW+YGSIWDASSWATEDGKYKADYKYQPF  +Y NFK  GCT   +
Sbjct: 188 RYPKKSASTFPLRPMWLYGSIWDASSWATEDGKYKADYKYQPFTAKYTNFKALGCTAYSS 247

Query: 245 ASCRPPSTGASGGEGLSQQQEKAMEWVQNNYLVYNYCHDPKRDHTLIPEC 295
           A C P S       GL++QQ +AM WVQ + +VYNYC D KRDH+L PEC
Sbjct: 248 ARCYPLSASPYRSGGLTRQQHQAMRWVQTHSMVYNYCKDYKRDHSLTPEC 297

BLAST of MS006835 vs. ExPASy Swiss-Prot
Match: Q38908 (Probable xyloglucan endotransglucosylase/hydrolase protein 30 OS=Arabidopsis thaliana OX=3702 GN=XTH30 PE=2 SV=2)

HSP 1 Score: 221.9 bits (564), Expect = 9.8e-57
Identity = 111/270 (41.11%), Postives = 157/270 (58.15%), Query Frame = 0

Query: 35  IQTIGFDQGFRNLWGSQH--QRLDHGALTIWLDATSGSGFKSLRRYQSGYFGAAIKLHPG 94
           + T+ F++    L+G  +  +  D  ++ + LD  +GSGF S   YQ G++ + IKL   
Sbjct: 27  LNTLSFEESLSPLFGDANLVRSPDDLSVRLLLDRYTGSGFISSNMYQHGFYSSMIKLPAD 86

Query: 95  YTAGVITSLYLSNNEDFPGYHDEIDIEFLGTTSDKPYILQTNVYMRGSGDGNIIGREMRF 154
           YTAGV+ + Y SN + F   HDE+DIEFLG    KP+  QTN+Y  GS      GRE R+
Sbjct: 87  YTAGVVVAFYTSNGDVFEKTHDELDIEFLGNIKGKPWRFQTNLYGNGSTHR---GREERY 146

Query: 155 HLWFDPTQDFHNYAISWTPREIIFFVDDVPIRRYERKS--DATFPVRPMWVYGSIWDASS 214
            LWFDP+++FH Y+I WTP +IIF+VDDVPIR   R     A +P +PM +Y +IWDAS 
Sbjct: 147 RLWFDPSKEFHRYSILWTPHKIIFWVDDVPIREVIRNDAMGADYPAKPMALYATIWDASD 206

Query: 215 WATEDGKYKADYKYQPFIGRYNNFKLGGCTVDGA------ASCRPPSTGASGGEGLSQQQ 274
           WAT  GKYKA+YK+ PF+  + +F L GC+VD         S       +     ++  Q
Sbjct: 207 WATSGGKYKANYKFAPFVAEFKSFSLDGCSVDPIQEVPMDCSDSVDFLESQDYSSINSHQ 266

Query: 275 EKAMEWVQNNYLVYNYCHDPKRDHTLIPEC 295
             AM   +  ++ Y+YC+D  R    +PEC
Sbjct: 267 RAAMRRFRQRFMYYSYCYDTLRYPEPLPEC 293

BLAST of MS006835 vs. ExPASy Swiss-Prot
Match: Q8LDS2 (Probable xyloglucan endotransglucosylase/hydrolase protein 27 OS=Arabidopsis thaliana OX=3702 GN=XTH27 PE=2 SV=2)

HSP 1 Score: 215.7 bits (548), Expect = 7.0e-55
Identity = 119/303 (39.27%), Postives = 171/303 (56.44%), Query Frame = 0

Query: 2   MSRLLVLLSLPILFLSLSSCQNPPSPGYHPSNQIQTIGFDQGFRNLWGSQ----HQRLDH 61
           +SRLLV +SL    +S  + QN P              F++ +  L+G +    HQ  D 
Sbjct: 4   LSRLLVFMSLFSGLVSGFALQNLP-----------ITSFEESYTQLFGDKNLFVHQ--DG 63

Query: 62  GALTIWLDATSGSGFKSLRRYQSGYFGAAIKLHPGYTAGVITSLYLSNNEDFPGYHDEID 121
            ++ + LD  +GSGF S   Y  G+F A+IKL   YTAGV+ + Y+SN + +   HDEID
Sbjct: 64  KSVRLTLDERTGSGFVSNDYYLHGFFSASIKLPSDYTAGVVVAFYMSNGDMYEKNHDEID 123

Query: 122 IEFLGTTSDKPYILQTNVYMRGSGDGNIIGREMRFHLWFDPTQDFHNYAISWTPREIIFF 181
            EFLG   +K + +QTN+Y  GS      GRE R++LWFDPT+DFH Y+I W+   IIFF
Sbjct: 124 FEFLGNIREKEWRVQTNIYGNGSTHS---GREERYNLWFDPTEDFHQYSILWSDSHIIFF 183

Query: 182 VDDVPIRRYERKSD--ATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFK 241
           VD+VPIR  +R ++    FP +PM +Y +IWD S WAT  GKY  +YKY P+I R+++  
Sbjct: 184 VDNVPIREVKRTAEMGGHFPSKPMSLYTTIWDGSKWATNGGKYGVNYKYAPYIARFSDLV 243

Query: 242 LGGCTVDGAASCRPPSTGAS----GGEGLSQQQEKAMEWVQNNYLVYNYCHDPKRDHTLI 295
           L GC VD          GA+      + ++  Q   M+  +   + Y+YC+D  R +  +
Sbjct: 244 LHGCPVDPIEQFPRCDEGAAEDMRAAQEITPSQRSKMDVFRRRLMTYSYCYDRARYNVAL 290

BLAST of MS006835 vs. ExPASy Swiss-Prot
Match: Q8L7H3 (Probable xyloglucan endotransglucosylase/hydrolase protein 29 OS=Arabidopsis thaliana OX=3702 GN=XTH29 PE=2 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.6e-54
Identity = 112/281 (39.86%), Postives = 157/281 (55.87%), Query Frame = 0

Query: 35  IQTIGFDQGFRNLWGSQH--QRLDHGALTIWLDATSGSGFKSLRRYQSGYFGAAIKLHPG 94
           I  I FD+G  +L+G  +  +  D  ++ + LD  +GSGF S   YQ G+F + IKL   
Sbjct: 35  INPIFFDEGLSHLFGEGNLIRSPDDRSVRLLLDKYTGSGFISSSMYQHGFFSSLIKLPGA 94

Query: 95  YTAGVITSLYLSNNEDFPGYHDEIDIEFLGTTSDKPYILQTNVYMRGSGDGNIIGREMRF 154
           YTAG++ + Y SN + F   HDE+DIEFLG    KP+  QTN+Y  GS +    GRE R+
Sbjct: 95  YTAGIVVAFYTSNGDVFVKDHDELDIEFLGNLEGKPWRFQTNMYGNGSTNR---GREERY 154

Query: 155 HLWFDPTQDFHNYAISWTPREIIFFVDDVPIRRYERKSD--ATFPVRPMWVYGSIWDASS 214
            LWFDP+++FH Y+I WTP +IIF+VDDVPIR   RK +    +P +PM +Y +IWDASS
Sbjct: 155 RLWFDPSKEFHRYSILWTPTKIIFWVDDVPIREILRKEEMNGDYPQKPMSLYATIWDASS 214

Query: 215 WATEDGKYKADYKYQPFIGRYNNFKLGGCTVDGA-----------------ASCRPPSTG 274
           WAT  GK+  DY + PF+  + +  L GC V  +                  S       
Sbjct: 215 WATSGGKFGVDYTFSPFVSEFKDIALDGCNVSDSFPGENNNNNIGNYNNINCSVSDQFLM 274

Query: 275 ASGGEGLSQQQEKAMEWVQNNYLVYNYCHDPKRDHTLIPEC 295
           ++    +S +Q  AM   +  Y+ Y+YC+D  R     PEC
Sbjct: 275 SNDYSTISPKQATAMRRFRERYMYYSYCYDTIRYSVPPPEC 312

BLAST of MS006835 vs. ExPASy TrEMBL
Match: A0A6J1GC21 (Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111452608 PE=3 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 2.0e-145
Identity = 240/293 (81.91%), Postives = 262/293 (89.42%), Query Frame = 0

Query: 2   MSRLLVLLSLPILFLSLSSCQNPPSPGYHPSNQIQTIGFDQGFRNLWGSQHQRLDHGALT 61
           M+  LVLL    +F S SS  NPPSPG++PSNQ+Q+IGFD GFRN WGSQHQ++DHGALT
Sbjct: 1   MAHFLVLL---FVFFSCSSALNPPSPGFYPSNQVQSIGFDHGFRNRWGSQHQKVDHGALT 60

Query: 62  IWLDATSGSGFKSLRRYQSGYFGAAIKLHPGYTAGVITSLYLSNNEDFPGYHDEIDIEFL 121
           IWLD++SGSGFKSL RY+SGYFGAAIKLHPGYTAGVITS YLSNNED+PG HDEIDIEFL
Sbjct: 61  IWLDSSSGSGFKSLHRYKSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGNHDEIDIEFL 120

Query: 122 GTTSDKPYILQTNVYMRGSGDGNIIGREMRFHLWFDPTQDFHNYAISWTPREIIFFVDDV 181
           GTT  KPY+LQTNV+MRGSGDGNIIGREMRFHLWFDPTQDFHNYAI WTP EIIF VDDV
Sbjct: 121 GTTPGKPYVLQTNVFMRGSGDGNIIGREMRFHLWFDPTQDFHNYAILWTPEEIIFLVDDV 180

Query: 182 PIRRYERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCTV 241
           PIRRY +KS+ATFP+RPMWVYGSIWDASSWATEDGKYKADY+YQPFIGRYNNFKLGGC V
Sbjct: 181 PIRRYVKKSEATFPIRPMWVYGSIWDASSWATEDGKYKADYRYQPFIGRYNNFKLGGCAV 240

Query: 242 DGAASCRPPSTGASGGEGLSQQQEKAMEWVQNNYLVYNYCHDPKRDHTLIPEC 295
           DGAA+CR  S+G  GG GLSQQQEKAM WVQ NYL+YNYCHDP+RDHT  PEC
Sbjct: 241 DGAATCRRVSSG-PGGRGLSQQQEKAMAWVQGNYLMYNYCHDPRRDHTSTPEC 289

BLAST of MS006835 vs. ExPASy TrEMBL
Match: A0A0A0LV02 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G109370 PE=3 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 5.8e-145
Identity = 245/293 (83.62%), Postives = 261/293 (89.08%), Query Frame = 0

Query: 6   LVLLSL-PILFLSLSSCQN-PPSPGYHPSNQIQTIGFDQGFRNLWGSQHQRLDHGALTIW 65
           L LLSL    F S +S QN PPSPGY PS Q+Q+ GFDQ FRN WGSQHQ++D G LTIW
Sbjct: 7   LFLLSLFFFFFFSFTSSQNYPPSPGYFPSTQVQSTGFDQVFRNRWGSQHQKVDQGTLTIW 66

Query: 66  LDATSGSGFKSLRRYQSGYFGAAIKLHPGYTAGVITSLYLSNNEDFPGYHDEIDIEFLGT 125
           LD++SGSGFKSL RYQSGYFGAAIKLHPGYTAGVITS YLSNNED+PG HDEIDIEFLGT
Sbjct: 67  LDSSSGSGFKSLHRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGNHDEIDIEFLGT 126

Query: 126 TSDKPYILQTNVYMRGSGDGNIIGREMRFHLWFDPTQDFHNYAISWTPREIIFFVDDVPI 185
           TSDKPY+LQTNV+MRGSGDGNIIGREMRFHLWF+PTQDFHNYAI WTP EIIF VDDVPI
Sbjct: 127 TSDKPYVLQTNVFMRGSGDGNIIGREMRFHLWFNPTQDFHNYAILWTPEEIIFLVDDVPI 186

Query: 186 RRYERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCTVDG 245
           RRYERKS+ATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKL GCT DG
Sbjct: 187 RRYERKSEATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLSGCTTDG 246

Query: 246 AASCRPPSTGASGG--EGLSQQQEKAMEWVQNNYLVYNYCHDPKRDHTLIPEC 295
           AASCRP ++G  GG    +SQQQEKAMEWVQNNYLVYNYCHDP+RDHTL PEC
Sbjct: 247 AASCRPLNSGPGGGGRGRMSQQQEKAMEWVQNNYLVYNYCHDPRRDHTLTPEC 299

BLAST of MS006835 vs. ExPASy TrEMBL
Match: A0A6J1HM52 (Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111464871 PE=3 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 1.3e-144
Identity = 239/289 (82.70%), Postives = 259/289 (89.62%), Query Frame = 0

Query: 6   LVLLSLPILFLSLSSCQNPPSPGYHPSNQIQTIGFDQGFRNLWGSQHQRLDHGALTIWLD 65
           L+LLSL  LF S  S QNPPSPGY PSNQ+Q+I FD GFRN WGSQHQ++D G LTIWLD
Sbjct: 6   LILLSL-FLFFSFISSQNPPSPGYFPSNQVQSIAFDHGFRNRWGSQHQKVDQGTLTIWLD 65

Query: 66  ATSGSGFKSLRRYQSGYFGAAIKLHPGYTAGVITSLYLSNNEDFPGYHDEIDIEFLGTTS 125
           ++SGSGFKSL RYQSGYFGAAIKLHPGYTAGVITS YLSNNED+PG HDEIDIEFLGTTS
Sbjct: 66  SSSGSGFKSLNRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGNHDEIDIEFLGTTS 125

Query: 126 DKPYILQTNVYMRGSGDGNIIGREMRFHLWFDPTQDFHNYAISWTPREIIFFVDDVPIRR 185
           DKPY+LQTNV+MRGSGDGNIIGREMRFHLWFDPTQDFHNYAI WT  +IIF VDDVPIRR
Sbjct: 126 DKPYVLQTNVFMRGSGDGNIIGREMRFHLWFDPTQDFHNYAILWTREQIIFLVDDVPIRR 185

Query: 186 YERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCTVDGAA 245
           YE+K++AT+PVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGC  DGA+
Sbjct: 186 YEKKTEATYPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCAADGAS 245

Query: 246 SCRPPSTGASGGEGLSQQQEKAMEWVQNNYLVYNYCHDPKRDHTLIPEC 295
           SC P S G   G  +SQQQE+AMEWVQ+NYLVYNYCHDP+RDHTL PEC
Sbjct: 246 SCHPLSGGP--GSWMSQQQERAMEWVQSNYLVYNYCHDPRRDHTLTPEC 291

BLAST of MS006835 vs. ExPASy TrEMBL
Match: A0A6J1KFJ7 (Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111493408 PE=3 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 2.2e-144
Identity = 239/293 (81.57%), Postives = 260/293 (88.74%), Query Frame = 0

Query: 2   MSRLLVLLSLPILFLSLSSCQNPPSPGYHPSNQIQTIGFDQGFRNLWGSQHQRLDHGALT 61
           M+  LVLL     F S SS  NPPSPG+ PSNQ+Q+IGFDQGFRN WGS HQ++DHGALT
Sbjct: 1   MAHFLVLL---FAFFSCSSALNPPSPGFSPSNQVQSIGFDQGFRNRWGSHHQKVDHGALT 60

Query: 62  IWLDATSGSGFKSLRRYQSGYFGAAIKLHPGYTAGVITSLYLSNNEDFPGYHDEIDIEFL 121
           IWLD++SGSGFKSL RY+SGYFGAAIKLHPGYTAGVITS YLSNNED+PG HDEIDIEFL
Sbjct: 61  IWLDSSSGSGFKSLHRYKSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGNHDEIDIEFL 120

Query: 122 GTTSDKPYILQTNVYMRGSGDGNIIGREMRFHLWFDPTQDFHNYAISWTPREIIFFVDDV 181
           GTT  KPY+LQTNV+MRGSGDGNIIGREMRFHLWFDPTQDFHNYAI WTP EIIF VDDV
Sbjct: 121 GTTPGKPYVLQTNVFMRGSGDGNIIGREMRFHLWFDPTQDFHNYAILWTPNEIIFLVDDV 180

Query: 182 PIRRYERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCTV 241
           PIRRY +KS+ATFP+RPMWVYGSIWDASSWATEDGKYKADY++QPFIGRYNNFKLGGCTV
Sbjct: 181 PIRRYVKKSEATFPIRPMWVYGSIWDASSWATEDGKYKADYRHQPFIGRYNNFKLGGCTV 240

Query: 242 DGAASCRPPSTGASGGEGLSQQQEKAMEWVQNNYLVYNYCHDPKRDHTLIPEC 295
           DG A+CR  S+G  GG GLSQQQEKAM WVQ NYL+YNYCHDP+RDHT  PEC
Sbjct: 241 DGVATCRRVSSG-PGGRGLSQQQEKAMAWVQRNYLMYNYCHDPRRDHTSTPEC 289

BLAST of MS006835 vs. ExPASy TrEMBL
Match: A0A6J1I2D8 (Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111469279 PE=3 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 3.8e-144
Identity = 238/289 (82.35%), Postives = 258/289 (89.27%), Query Frame = 0

Query: 6   LVLLSLPILFLSLSSCQNPPSPGYHPSNQIQTIGFDQGFRNLWGSQHQRLDHGALTIWLD 65
           L+LLSL   F S +S QNPPSPGY PSNQ+Q+I FD GF N WGSQHQ++D G LTIWLD
Sbjct: 6   LILLSL-FPFFSFTSSQNPPSPGYFPSNQVQSIAFDHGFLNRWGSQHQKVDQGTLTIWLD 65

Query: 66  ATSGSGFKSLRRYQSGYFGAAIKLHPGYTAGVITSLYLSNNEDFPGYHDEIDIEFLGTTS 125
           ++SGSGFKSL RYQSGYFGAAIKLHPGYTAGVITS YLSNNED+PG HDEIDIEFLGTTS
Sbjct: 66  SSSGSGFKSLNRYQSGYFGAAIKLHPGYTAGVITSFYLSNNEDYPGNHDEIDIEFLGTTS 125

Query: 126 DKPYILQTNVYMRGSGDGNIIGREMRFHLWFDPTQDFHNYAISWTPREIIFFVDDVPIRR 185
           DKPY+LQTNV+MRGSGDGNIIGREMRFHLWFDPTQDFHNYAI WT  +IIF VDDVPIRR
Sbjct: 126 DKPYVLQTNVFMRGSGDGNIIGREMRFHLWFDPTQDFHNYAILWTREQIIFLVDDVPIRR 185

Query: 186 YERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCTVDGAA 245
           YE+K++AT+PVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGC  DGA 
Sbjct: 186 YEKKTEATYPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCAADGAV 245

Query: 246 SCRPPSTGASGGEGLSQQQEKAMEWVQNNYLVYNYCHDPKRDHTLIPEC 295
           SC P S G   G G+SQQQE+AMEWVQ+NYLVYNYCHDP+RDHTL PEC
Sbjct: 246 SCHPLSGGP--GSGMSQQQERAMEWVQSNYLVYNYCHDPRRDHTLTPEC 291

BLAST of MS006835 vs. TAIR 10
Match: AT3G44990.1 (xyloglucan endo-transglycosylase-related 8 )

HSP 1 Score: 436.8 bits (1122), Expect = 1.4e-122
Identity = 194/293 (66.21%), Postives = 233/293 (79.52%), Query Frame = 0

Query: 2   MSRLLVLLSLPILFLSLSSCQNPPSPGYHPSNQIQTIGFDQGFRNLWGSQHQRLDHGALT 61
           M+  L+ L+L +L  S    Q  PSPGY+PS+++ T  FD+ FR LWGSQHQR +   +T
Sbjct: 1   MALSLIFLALLVLCPSSGHSQRSPSPGYYPSSRVPTSPFDREFRTLWGSQHQRREQDVVT 60

Query: 62  IWLDATSGSGFKSLRRYQSGYFGAAIKLHPGYTAGVITSLYLSNNEDFPGYHDEIDIEFL 121
           +WLD ++GSGFKSLR Y+SGYFGA+IKL PG+TAGV TSLYLSNN++ PG HDE+DIEFL
Sbjct: 61  LWLDKSTGSGFKSLRPYRSGYFGASIKLQPGFTAGVDTSLYLSNNQEHPGDHDEVDIEFL 120

Query: 122 GTTSDKPYILQTNVYMRGSGDGNIIGREMRFHLWFDPTQDFHNYAISWTPREIIFFVDDV 181
           GTT  KPY LQTNV++RGSGD N+IGREM+F LWFDPTQDFH+YAI W P +I+FFVDDV
Sbjct: 121 GTTPGKPYSLQTNVFVRGSGDRNVIGREMKFTLWFDPTQDFHHYAILWNPNQIVFFVDDV 180

Query: 182 PIRRYERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCTV 241
           PIR Y RK++A FP RPMWVYGSIWDAS WATE+G+ KADY+YQPF+ +Y NFKL GCT 
Sbjct: 181 PIRTYNRKNEAIFPTRPMWVYGSIWDASDWATENGRIKADYRYQPFVAKYKNFKLAGCTA 240

Query: 242 DGAASCRPPSTGASGGEGLSQQQEKAMEWVQNNYLVYNYCHDPKRDHTLIPEC 295
           D ++SCRPPS       GLS+QQ  A+ W Q N+LVYNYCHDPKRDHT  PEC
Sbjct: 241 DSSSSCRPPSPAPMRNRGLSRQQMAALTWAQRNFLVYNYCHDPKRDHTQTPEC 293

BLAST of MS006835 vs. TAIR 10
Match: AT2G36870.1 (xyloglucan endotransglucosylase/hydrolase 32 )

HSP 1 Score: 433.0 bits (1112), Expect = 2.0e-121
Identity = 198/290 (68.28%), Postives = 233/290 (80.34%), Query Frame = 0

Query: 5   LLVLLSLPILFLSLSSCQNPPSPGYHPSNQIQTIGFDQGFRNLWGSQHQRLDHGALTIWL 64
           LL +  L +L+ S  +   PPSPGY PS+++ ++ F +GFRNLWG QHQR+D  ALTIWL
Sbjct: 8   LLSIFHLLVLWGSSVNAYWPPSPGYWPSSKVGSLNFYKGFRNLWGPQHQRMDQNALTIWL 67

Query: 65  DATSGSGFKSLRRYQSGYFGAAIKLHPGYTAGVITSLYLSNNEDFPGYHDEIDIEFLGTT 124
           D TSGSGFKS++ ++SGYFGA IKL PGYTAGVITSLYLSNNE  PG+HDE+DIEFLGTT
Sbjct: 68  DRTSGSGFKSVKPFRSGYFGANIKLQPGYTAGVITSLYLSNNEAHPGFHDEVDIEFLGTT 127

Query: 125 SDKPYILQTNVYMRGSGDGNIIGREMRFHLWFDPTQDFHNYAISWTPREIIFFVDDVPIR 184
             KPY LQTNVY+RGSGDG IIGREM+F LWFDPT+DFH+YAI W+PREIIF VDD+PIR
Sbjct: 128 FGKPYTLQTNVYIRGSGDGKIIGREMKFRLWFDPTKDFHHYAILWSPREIIFLVDDIPIR 187

Query: 185 RYERKSDATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFKLGGCTVDGA 244
           RY +KS +TFP+RPMW+YGSIWDASSWATEDGKYKADYKYQPF  +Y NFK  GCT   +
Sbjct: 188 RYPKKSASTFPLRPMWLYGSIWDASSWATEDGKYKADYKYQPFTAKYTNFKALGCTAYSS 247

Query: 245 ASCRPPSTGASGGEGLSQQQEKAMEWVQNNYLVYNYCHDPKRDHTLIPEC 295
           A C P S       GL++QQ +AM WVQ + +VYNYC D KRDH+L PEC
Sbjct: 248 ARCYPLSASPYRSGGLTRQQHQAMRWVQTHSMVYNYCKDYKRDHSLTPEC 297

BLAST of MS006835 vs. TAIR 10
Match: AT2G36870.2 (xyloglucan endotransglucosylase/hydrolase 32 )

HSP 1 Score: 258.5 bits (659), Expect = 6.7e-69
Identity = 119/171 (69.59%), Postives = 142/171 (83.04%), Query Frame = 0

Query: 5   LLVLLSLPILFLSLSSCQNPPSPGYHPSNQIQTIGFDQGFRNLWGSQHQRLDHGALTIWL 64
           LL +  L +L+ S  +   PPSPGY PS+++ ++ F +GFRNLWG QHQR+D  ALTIWL
Sbjct: 8   LLSIFHLLVLWGSSVNAYWPPSPGYWPSSKVGSLNFYKGFRNLWGPQHQRMDQNALTIWL 67

Query: 65  DATSGSGFKSLRRYQSGYFGAAIKLHPGYTAGVITSLYLSNNEDFPGYHDEIDIEFLGTT 124
           D TSGSGFKS++ ++SGYFGA IKL PGYTAGVITSLYLSNNE  PG+HDE+DIEFLGTT
Sbjct: 68  DRTSGSGFKSVKPFRSGYFGANIKLQPGYTAGVITSLYLSNNEAHPGFHDEVDIEFLGTT 127

Query: 125 SDKPYILQTNVYMRGSGDGNIIGREMRFHLWFDPTQDFHNYAISWTPREII 176
             KPY LQTNVY+RGSGDG IIGREM+F LWFDPT+DFH+YAI W+PREI+
Sbjct: 128 FGKPYTLQTNVYIRGSGDGKIIGREMKFRLWFDPTKDFHHYAILWSPREIM 178

BLAST of MS006835 vs. TAIR 10
Match: AT1G32170.1 (xyloglucan endotransglucosylase/hydrolase 30 )

HSP 1 Score: 221.9 bits (564), Expect = 7.0e-58
Identity = 111/270 (41.11%), Postives = 157/270 (58.15%), Query Frame = 0

Query: 35  IQTIGFDQGFRNLWGSQH--QRLDHGALTIWLDATSGSGFKSLRRYQSGYFGAAIKLHPG 94
           + T+ F++    L+G  +  +  D  ++ + LD  +GSGF S   YQ G++ + IKL   
Sbjct: 27  LNTLSFEESLSPLFGDANLVRSPDDLSVRLLLDRYTGSGFISSNMYQHGFYSSMIKLPAD 86

Query: 95  YTAGVITSLYLSNNEDFPGYHDEIDIEFLGTTSDKPYILQTNVYMRGSGDGNIIGREMRF 154
           YTAGV+ + Y SN + F   HDE+DIEFLG    KP+  QTN+Y  GS      GRE R+
Sbjct: 87  YTAGVVVAFYTSNGDVFEKTHDELDIEFLGNIKGKPWRFQTNLYGNGSTHR---GREERY 146

Query: 155 HLWFDPTQDFHNYAISWTPREIIFFVDDVPIRRYERKS--DATFPVRPMWVYGSIWDASS 214
            LWFDP+++FH Y+I WTP +IIF+VDDVPIR   R     A +P +PM +Y +IWDAS 
Sbjct: 147 RLWFDPSKEFHRYSILWTPHKIIFWVDDVPIREVIRNDAMGADYPAKPMALYATIWDASD 206

Query: 215 WATEDGKYKADYKYQPFIGRYNNFKLGGCTVDGA------ASCRPPSTGASGGEGLSQQQ 274
           WAT  GKYKA+YK+ PF+  + +F L GC+VD         S       +     ++  Q
Sbjct: 207 WATSGGKYKANYKFAPFVAEFKSFSLDGCSVDPIQEVPMDCSDSVDFLESQDYSSINSHQ 266

Query: 275 EKAMEWVQNNYLVYNYCHDPKRDHTLIPEC 295
             AM   +  ++ Y+YC+D  R    +PEC
Sbjct: 267 RAAMRRFRQRFMYYSYCYDTLRYPEPLPEC 293

BLAST of MS006835 vs. TAIR 10
Match: AT2G01850.1 (endoxyloglucan transferase A3 )

HSP 1 Score: 215.7 bits (548), Expect = 5.0e-56
Identity = 119/303 (39.27%), Postives = 171/303 (56.44%), Query Frame = 0

Query: 2   MSRLLVLLSLPILFLSLSSCQNPPSPGYHPSNQIQTIGFDQGFRNLWGSQ----HQRLDH 61
           +SRLLV +SL    +S  + QN P              F++ +  L+G +    HQ  D 
Sbjct: 4   LSRLLVFMSLFSGLVSGFALQNLP-----------ITSFEESYTQLFGDKNLFVHQ--DG 63

Query: 62  GALTIWLDATSGSGFKSLRRYQSGYFGAAIKLHPGYTAGVITSLYLSNNEDFPGYHDEID 121
            ++ + LD  +GSGF S   Y  G+F A+IKL   YTAGV+ + Y+SN + +   HDEID
Sbjct: 64  KSVRLTLDERTGSGFVSNDYYLHGFFSASIKLPSDYTAGVVVAFYMSNGDMYEKNHDEID 123

Query: 122 IEFLGTTSDKPYILQTNVYMRGSGDGNIIGREMRFHLWFDPTQDFHNYAISWTPREIIFF 181
            EFLG   +K + +QTN+Y  GS      GRE R++LWFDPT+DFH Y+I W+   IIFF
Sbjct: 124 FEFLGNIREKEWRVQTNIYGNGSTHS---GREERYNLWFDPTEDFHQYSILWSDSHIIFF 183

Query: 182 VDDVPIRRYERKSD--ATFPVRPMWVYGSIWDASSWATEDGKYKADYKYQPFIGRYNNFK 241
           VD+VPIR  +R ++    FP +PM +Y +IWD S WAT  GKY  +YKY P+I R+++  
Sbjct: 184 VDNVPIREVKRTAEMGGHFPSKPMSLYTTIWDGSKWATNGGKYGVNYKYAPYIARFSDLV 243

Query: 242 LGGCTVDGAASCRPPSTGAS----GGEGLSQQQEKAMEWVQNNYLVYNYCHDPKRDHTLI 295
           L GC VD          GA+      + ++  Q   M+  +   + Y+YC+D  R +  +
Sbjct: 244 LHGCPVDPIEQFPRCDEGAAEDMRAAQEITPSQRSKMDVFRRRLMTYSYCYDRARYNVAL 290

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038894127.18.6e-15185.71probable xyloglucan endotransglucosylase/hydrolase protein 32 [Benincasa hispida... [more]
XP_023519902.11.4e-14583.39probable xyloglucan endotransglucosylase/hydrolase protein 32 isoform X1 [Cucurb... [more]
XP_022949180.14.1e-14581.91xyloglucan endotransglucosylase/hydrolase protein 31-like [Cucurbita moschata] >... [more]
KAG6583568.17.0e-14582.70putative xyloglucan endotransglucosylase/hydrolase protein 32, partial [Cucurbit... [more]
XP_004147295.21.2e-14483.62xyloglucan endotransglucosylase/hydrolase protein 31 [Cucumis sativus] >KGN64814... [more]
Match NameE-valueIdentityDescription
P930461.9e-12166.21Xyloglucan endotransglucosylase/hydrolase protein 31 OS=Arabidopsis thaliana OX=... [more]
Q9SJL92.8e-12068.28Probable xyloglucan endotransglucosylase/hydrolase protein 32 OS=Arabidopsis tha... [more]
Q389089.8e-5741.11Probable xyloglucan endotransglucosylase/hydrolase protein 30 OS=Arabidopsis tha... [more]
Q8LDS27.0e-5539.27Probable xyloglucan endotransglucosylase/hydrolase protein 27 OS=Arabidopsis tha... [more]
Q8L7H31.6e-5439.86Probable xyloglucan endotransglucosylase/hydrolase protein 29 OS=Arabidopsis tha... [more]
Match NameE-valueIdentityDescription
A0A6J1GC212.0e-14581.91Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A0A0LV025.8e-14583.62Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G10... [more]
A0A6J1HM521.3e-14482.70Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1KFJ72.2e-14481.57Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1I2D83.8e-14482.35Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT3G44990.11.4e-12266.21xyloglucan endo-transglycosylase-related 8 [more]
AT2G36870.12.0e-12168.28xyloglucan endotransglucosylase/hydrolase 32 [more]
AT2G36870.26.7e-6969.59xyloglucan endotransglucosylase/hydrolase 32 [more]
AT1G32170.17.0e-5841.11xyloglucan endotransglucosylase/hydrolase 30 [more]
AT2G01850.15.0e-5639.27endoxyloglucan transferase A3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000757Glycoside hydrolase family 16PFAMPF00722Glyco_hydro_16coord: 41..223
e-value: 1.5E-53
score: 180.9
IPR000757Glycoside hydrolase family 16PROSITEPS51762GH16_2coord: 19..231
score: 30.25275
NoneNo IPR availableGENE3D2.60.120.200coord: 20..294
e-value: 2.9E-99
score: 333.8
NoneNo IPR availablePANTHERPTHR31062:SF146XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 31coord: 7..294
IPR016455Xyloglucan endotransglucosylase/hydrolasePIRSFPIRSF005604EndGlu_transfcoord: 4..294
e-value: 2.0E-99
score: 330.4
IPR010713Xyloglucan endo-transglycosylase, C-terminalPFAMPF06955XET_Ccoord: 258..294
e-value: 1.8E-14
score: 53.3
IPR044791Beta-glucanase/XTHPANTHERPTHR31062XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATEDcoord: 7..294
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 62..281

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS006835.1MS006835.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006073 cellular glucan metabolic process
biological_process GO:0042546 cell wall biogenesis
biological_process GO:0010411 xyloglucan metabolic process
cellular_component GO:0048046 apoplast
cellular_component GO:0005618 cell wall
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0016762 xyloglucan:xyloglucosyl transferase activity