MS006811 (gene) Bitter gourd (TR) v1

Overview
NameMS006811
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionRNase H domain-containing protein
Locationscaffold60: 694655 .. 694955 (-)
RNA-Seq ExpressionMS006811
SyntenyMS006811
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGTCATGGGTTCCTCCGACTGCTGATAGCCTGAAGCTAAACACGGATGCCGCATGCAATGAGACGATTCAAGGTATCGGTGTGTGTTTTATTTTTCGGGACTCTTCTGGTACGATTTGTGCAACGGGATCCTTCTTCCACCCATTCTATCTTTCGCCACTTTTAGGAGCTTCTCGCAATTATACAAGGATTGGAACAGACTCAAACTCTTAATTGCAGGAAAATTATGGTGGAATCAGATTCCCTTCAAGCAATTTCTCTTTTTCAACAAAAGTCGAAATCTTTGGCAGCCTCAAGCGTC

mRNA sequence

CTGTCATGGGTTCCTCCGACTGCTGATAGCCTGAAGCTAAACACGGATGCCGCATGCAATGAGACGATTCAAGGTATCGGTGTGTGTTTTATTTTTCGGGACTCTTCTGGTACGATTTGTGCAACGGGATCCTTCTTCCACCCATTCTATCTTTCGCCACTTGAGCTTCTCGCAATTATACAAGGATTGGAACAGACTCAAACTCTTAATTGCAGGAAAATTATGGTGGAATCAGATTCCCTTCAAGCAATTTCTCTTTTTCAACAAAAGTCGAAATCTTTGGCAGCCTCAAGCGTC

Coding sequence (CDS)

CTGTCATGGGTTCCTCCGACTGCTGATAGCCTGAAGCTAAACACGGATGCCGCATGCAATGAGACGATTCAAGGTATCGGTGTGTGTTTTATTTTTCGGGACTCTTCTGGTACGATTTGTGCAACGGGATCCTTCTTCCACCCATTCTATCTTTCGCCACTTGAGCTTCTCGCAATTATACAAGGATTGGAACAGACTCAAACTCTTAATTGCAGGAAAATTATGGTGGAATCAGATTCCCTTCAAGCAATTTCTCTTTTTCAACAAAAGTCGAAATCTTTGGCAGCCTCAAGCGTC

Protein sequence

LSWVPPTADSLKLNTDAACNETIQGIGVCFIFRDSSGTICATGSFFHPFYLSPLELLAIIQGLEQTQTLNCRKIMVESDSLQAISLFQQKSKSLAASSV
Homology
BLAST of MS006811 vs. NCBI nr
Match: XP_022154990.1 (uncharacterized protein LOC111022134 isoform X1 [Momordica charantia])

HSP 1 Score: 62.4 bits (150), Expect = 2.6e-06
Identity = 37/92 (40.22%), Postives = 52/92 (56.52%), Query Frame = 0

Query: 3   WVPPTADSLKLNTDAACNETIQGIGVCFIFRDSSGTICATGS--FFHPFYLSPLELLAII 62
           W PPT++S KLNTDAA        G+ +I RD  G +  TG         ++ LE++AI 
Sbjct: 255 WKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEVMAIC 314

Query: 63  QGLEQTQTLNCRKIMVESDSLQAISLFQQKSK 93
           +GL   +  +CR I +ESDSL+AI L  +  K
Sbjct: 315 EGLRAIRQEHCRPIHLESDSLEAIHLLHRHVK 346

BLAST of MS006811 vs. NCBI nr
Match: XP_022154991.1 (uncharacterized protein LOC111022134 isoform X2 [Momordica charantia])

HSP 1 Score: 62.4 bits (150), Expect = 2.6e-06
Identity = 37/92 (40.22%), Postives = 52/92 (56.52%), Query Frame = 0

Query: 3   WVPPTADSLKLNTDAACNETIQGIGVCFIFRDSSGTICATGS--FFHPFYLSPLELLAII 62
           W PPT++S KLNTDAA        G+ +I RD  G +  TG         ++ LE++AI 
Sbjct: 220 WKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEVMAIC 279

Query: 63  QGLEQTQTLNCRKIMVESDSLQAISLFQQKSK 93
           +GL   +  +CR I +ESDSL+AI L  +  K
Sbjct: 280 EGLRAIRQEHCRPIHLESDSLEAIHLLHRHVK 311

BLAST of MS006811 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 61.6 bits (148), Expect = 4.3e-06
Identity = 40/90 (44.44%), Postives = 51/90 (56.67%), Query Frame = 0

Query: 3    WVPPTADSLKLNTDAACNETIQGIGVCFIFRDSSGTICATGSFFHPFYLSPL--ELLAII 62
            W P ++ SLKLNTDAAC       G   I RDSS ++ A  S   PF LSPL  E+  I+
Sbjct: 1294 WRPSSSVSLKLNTDAACRGASTSFGC--IIRDSSCSLVAATSIRVPFPLSPLLAEIRCIL 1353

Query: 63   QGLEQTQTLNCRKIMVESDSLQAISLFQQK 91
            +GL+     N   + VESDSL AI L + +
Sbjct: 1354 EGLKFAAASNFTHLEVESDSLLAIQLIRNE 1381

BLAST of MS006811 vs. NCBI nr
Match: XP_022131661.1 (uncharacterized protein LOC111004786 [Momordica charantia])

HSP 1 Score: 61.2 bits (147), Expect = 5.7e-06
Identity = 33/88 (37.50%), Postives = 52/88 (59.09%), Query Frame = 0

Query: 3   WVPPTADSLKLNTDAACNETIQGIGVCFIFRDSSGTICATGSFFHPFYLSPL--ELLAII 62
           W+PP   ++K+N+DAAC  T  G+G+  I RD  G +    S F P  L+PL  E+  I+
Sbjct: 115 WLPPRDSAMKVNSDAACRSTSTGLGL--IIRDHFGVLLVAKSMFLPMPLNPLFAEIRGIL 174

Query: 63  QGLEQTQTLNCRKIMVESDSLQAISLFQ 89
           + L+   + +  +++VESD  +AI L Q
Sbjct: 175 EALKLAASRSYTRLVVESDCQEAIRLVQ 200

BLAST of MS006811 vs. NCBI nr
Match: XP_024178556.2 (uncharacterized protein LOC112184522 [Rosa chinensis])

HSP 1 Score: 59.3 bits (142), Expect = 2.2e-05
Identity = 35/95 (36.84%), Postives = 50/95 (52.63%), Query Frame = 0

Query: 3    WVPPTADSLKLNTDAACNETIQGIGVCFIFRDSSGTICATGSFFHPFYLSPL--ELLAII 62
            W PP   +LKLN D A   +IQ  G   + R+  G   A  S+   F LSPL  ELLA+ 
Sbjct: 984  WFPPATGTLKLNVDGAFLSSIQYGGTGGVLRNDQGDFIAAFSYRAEFVLSPLHAELLALK 1043

Query: 63   QGLEQTQTLNCRKIMVESDSLQAISLFQQKSKSLA 96
             GL+    +N  K++VE+D L A+      ++ L+
Sbjct: 1044 YGLDFLHAMNVTKVVVETDCLVAVQAINSSTEDLS 1078

BLAST of MS006811 vs. ExPASy TrEMBL
Match: A0A6J1DL64 (uncharacterized protein LOC111022134 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022134 PE=4 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.2e-06
Identity = 37/92 (40.22%), Postives = 52/92 (56.52%), Query Frame = 0

Query: 3   WVPPTADSLKLNTDAACNETIQGIGVCFIFRDSSGTICATGS--FFHPFYLSPLELLAII 62
           W PPT++S KLNTDAA        G+ +I RD  G +  TG         ++ LE++AI 
Sbjct: 255 WKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEVMAIC 314

Query: 63  QGLEQTQTLNCRKIMVESDSLQAISLFQQKSK 93
           +GL   +  +CR I +ESDSL+AI L  +  K
Sbjct: 315 EGLRAIRQEHCRPIHLESDSLEAIHLLHRHVK 346

BLAST of MS006811 vs. ExPASy TrEMBL
Match: A0A6J1DQC9 (uncharacterized protein LOC111022134 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022134 PE=4 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.2e-06
Identity = 37/92 (40.22%), Postives = 52/92 (56.52%), Query Frame = 0

Query: 3   WVPPTADSLKLNTDAACNETIQGIGVCFIFRDSSGTICATGS--FFHPFYLSPLELLAII 62
           W PPT++S KLNTDAA        G+ +I RD  G +  TG         ++ LE++AI 
Sbjct: 220 WKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEVMAIC 279

Query: 63  QGLEQTQTLNCRKIMVESDSLQAISLFQQKSK 93
           +GL   +  +CR I +ESDSL+AI L  +  K
Sbjct: 280 EGLRAIRQEHCRPIHLESDSLEAIHLLHRHVK 311

BLAST of MS006811 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 2.1e-06
Identity = 40/90 (44.44%), Postives = 51/90 (56.67%), Query Frame = 0

Query: 3    WVPPTADSLKLNTDAACNETIQGIGVCFIFRDSSGTICATGSFFHPFYLSPL--ELLAII 62
            W P ++ SLKLNTDAAC       G   I RDSS ++ A  S   PF LSPL  E+  I+
Sbjct: 1294 WRPSSSVSLKLNTDAACRGASTSFGC--IIRDSSCSLVAATSIRVPFPLSPLLAEIRCIL 1353

Query: 63   QGLEQTQTLNCRKIMVESDSLQAISLFQQK 91
            +GL+     N   + VESDSL AI L + +
Sbjct: 1354 EGLKFAAASNFTHLEVESDSLLAIQLIRNE 1381

BLAST of MS006811 vs. ExPASy TrEMBL
Match: A0A6J1BQ49 (uncharacterized protein LOC111004786 OS=Momordica charantia OX=3673 GN=LOC111004786 PE=4 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 2.8e-06
Identity = 33/88 (37.50%), Postives = 52/88 (59.09%), Query Frame = 0

Query: 3   WVPPTADSLKLNTDAACNETIQGIGVCFIFRDSSGTICATGSFFHPFYLSPL--ELLAII 62
           W+PP   ++K+N+DAAC  T  G+G+  I RD  G +    S F P  L+PL  E+  I+
Sbjct: 115 WLPPRDSAMKVNSDAACRSTSTGLGL--IIRDHFGVLLVAKSMFLPMPLNPLFAEIRGIL 174

Query: 63  QGLEQTQTLNCRKIMVESDSLQAISLFQ 89
           + L+   + +  +++VESD  +AI L Q
Sbjct: 175 EALKLAASRSYTRLVVESDCQEAIRLVQ 200

BLAST of MS006811 vs. ExPASy TrEMBL
Match: J7G0Q7 (Non-ltr retroelement reverse transcriptase OS=Rosa rugosa OX=74645 PE=4 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 2.3e-05
Identity = 38/95 (40.00%), Postives = 48/95 (50.53%), Query Frame = 0

Query: 3    WVPPTADSLKLNTDAACNETIQGIGVCFIFRDSSGTICATG--SFFHPFYLSPLELLAII 62
            W  P++  LK+N D A N   +  G   I RD  G++   G  +F HP      ELLAI 
Sbjct: 1490 WQAPSSGQLKVNCDGAANFKDRCFGGGTIIRDEFGSLIVAGGKNFQHPVSSLVAELLAIK 1549

Query: 63   QGLEQTQTLNCRKIMVESDSLQAISLFQQKSKSLA 96
             GL+       R IMVESD L+AI L   K + LA
Sbjct: 1550 VGLDLVVERRLRNIMVESDCLEAIHLLNSKERCLA 1584

BLAST of MS006811 vs. TAIR 10
Match: AT5G65005.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 43.5 bits (101), Expect = 1.1e-04
Identity = 28/95 (29.47%), Postives = 45/95 (47.37%), Query Frame = 0

Query: 3   WVPPTADSLKLNTDAACNE--TIQGIGVCFIFRDSSGTI--CATGSFFHPFYLSPLELLA 62
           W PP  D LK N DA+ +E  T+ G+G  +I R+S GT+  C  G F         E   
Sbjct: 105 WSPPGRDKLKCNYDASHHERNTVSGLG--WILRNSQGTVIECGMGKFQGRMTTEEAECST 164

Query: 63  IIQGLEQTQTLNCRKIMVESDSLQAISLFQQKSKS 94
           +I  ++ +     +K++ E D+     +   KS +
Sbjct: 165 LIWAIQASYGFGHKKVIFEGDNQTITRMINTKSSN 197

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022154990.12.6e-0640.22uncharacterized protein LOC111022134 isoform X1 [Momordica charantia][more]
XP_022154991.12.6e-0640.22uncharacterized protein LOC111022134 isoform X2 [Momordica charantia][more]
XP_022158377.14.3e-0644.44uncharacterized protein LOC111024874 [Momordica charantia][more]
XP_022131661.15.7e-0637.50uncharacterized protein LOC111004786 [Momordica charantia][more]
XP_024178556.22.2e-0536.84uncharacterized protein LOC112184522 [Rosa chinensis][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DL641.2e-0640.22uncharacterized protein LOC111022134 isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1DQC91.2e-0640.22uncharacterized protein LOC111022134 isoform X2 OS=Momordica charantia OX=3673 G... [more]
A0A6J1DX302.1e-0644.44uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
A0A6J1BQ492.8e-0637.50uncharacterized protein LOC111004786 OS=Momordica charantia OX=3673 GN=LOC111004... [more]
J7G0Q72.3e-0540.00Non-ltr retroelement reverse transcriptase OS=Rosa rugosa OX=74645 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G65005.11.1e-0429.47Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 14..92
e-value: 9.1E-9
score: 35.2
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 9..97
e-value: 1.5E-8
score: 36.8
NoneNo IPR availablePANTHERPTHR47074:SF37PROTEIN, PUTATIVE-RELATEDcoord: 2..91
NoneNo IPR availablePANTHERPTHR47074BNAC02G40300D PROTEINcoord: 2..91
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 13..92
e-value: 2.26642E-14
score: 61.5612
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 11..89

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS006811.1MS006811.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity