MS006768 (gene) Bitter gourd (TR) v1

Overview


Name	MS006768
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	splicing factor U2af small subunit B-like
Location	scaffold60: 234845 .. 236359 (-)
RNA-Seq Expression	MS006768
Synteny	MS006768

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAGGTGCTGTAATGGCCTCCATTTCCGGGACGCAGATGGGCAGGGCTAACTGTCCCTTCTACTCCAAGGTCGGGGCCTGCCGCCATGGCGATCAGTGTTCCCGGTTGCACCCCAAGCCTCTCATAAGCCCCACACTCTTGCTTTCTAATATGTACCAGAGACTTGATACGATTAGCCCTGGTGTCGACGCGCGCGGAAATCCGATCGACCCTCGTAAGATCCAGCGCCATTTTGAGGTGATTTATTTTCCTTTTCGATCTTTTAATGTGTTCGGAAGTGTAAATTTTTGTTATGAATCTTGACATTTTGGGGGCCAATCTTGATTCGGACTCAATTTTATTTTGCCCTAGTGTTAGAGATTGTCTAATTTGGTTGATATTTAAGCGATCGTGTAATTTTGAATAGAGCTAGATAATACTCTCCTCTGTTACCCTGTTGCTGCATGTTCGTGGTGTGGTAATGAATTCTTGTATAGAAAAATGTTCTCCAAATATTACAAGAACCAAAATGAAAAGTGTTCCCATTATTTCTTTTGGAGGGCTTTGGTTCTTGATCTTGTGTTGTTTTGATTCTTTTTGCTGGTAGAAAAATTGTCGCCTAATTGTTGATTTGTGCACATTTTAGGAGTTCTATGAAGATATATTCGAGGAAATGAGCAAGTACGGTGAGATTGAGAGTTTGAATGTCTGTGACAACCTAGGTGACCACATGGTAAAAACTGTCTTCTCCCTCTGTTTATGTTCCATATTTTTGCATTTGTTATATGTTATTTCATTTGGTTAGGTGGGTAATGTTTATGTTCAATATCGAGAGGAGGGGCAAGCCGCAAATGCTCTTCGGAATCTTAGGGACAGATATTATGGTGGTTAGTTGGAGTTCCGTTTTCTTTTATGTATAATATGATCTGCTTTTACCATGTTTGCATAATTTTTATACAGCTTTCAATCTTGAATTTGGATGCGTAATTTCTGCAGGACTTCTAATTAGAGTCGACTTCTCTCCAGTGACAGACTTTATAGTAGCCGCTTGTAGACGATACGAAGAGAACATGTGTAATTTTGGTCCCTATTGTAATTTCATGCATCTGAAGAGGATAAGTTGGTATGCATATTTTCTTCACTTTCCCTTTTATGTAGTGTCTTTATATTGACTCTGAATTGCTGCTTCTGCTACGTGCACTTCATTTCAGGGAGCTGAGGAGCAGACTGTTTAGGACCAGGAGCCACAGCCCCGATAGGCATCGGAATTATGAACAACGCTCTTATGGTAGACATGGTCATAGTCGGAGGTATGATGACCGAGATGCTTATCATGAAAGCCGGAGCAGGAGGAGTAGGAGTACAAGCCCAGGTCGTAGAAGAAGCAGAAGTCGTAGTCTGCGGGGAAGGAGGAACCGAAGTCCAGTTAGAGAAGGGAGCGGAGAGAGGCGAGCTAGATTTGAGCAGTGGAACAGGGAAGGAGGACAGCAAGCAAATCATAACAATGCCAATTTTGATGGAAGTAAAAGTAGTCAT

mRNA sequence

AGAGGTGCTGTAATGGCCTCCATTTCCGGGACGCAGATGGGCAGGGCTAACTGTCCCTTCTACTCCAAGGTCGGGGCCTGCCGCCATGGCGATCAGTGTTCCCGGTTGCACCCCAAGCCTCTCATAAGCCCCACACTCTTGCTTTCTAATATGTACCAGAGACTTGATACGATTAGCCCTGGTGTCGACGCGCGCGGAAATCCGATCGACCCTCGTAAGATCCAGCGCCATTTTGAGGAGTTCTATGAAGATATATTCGAGGAAATGAGCAAGTACGGTGAGATTGAGAGTTTGAATGTCTGTGACAACCTAGGTGACCACATGGTGGGTAATGTTTATGTTCAATATCGAGAGGAGGGGCAAGCCGCAAATGCTCTTCGGAATCTTAGGGACAGATATTATGGTGGACTTCTAATTAGAGTCGACTTCTCTCCAGTGACAGACTTTATAGTAGCCGCTTGTAGACGATACGAAGAGAACATGTGTAATTTTGGTCCCTATTGTAATTTCATGCATCTGAAGAGGATAAGTTGGGAGCTGAGGAGCAGACTGTTTAGGACCAGGAGCCACAGCCCCGATAGGCATCGGAATTATGAACAACGCTCTTATGGTAGACATGGTCATAGTCGGAGGTATGATGACCGAGATGCTTATCATGAAAGCCGGAGCAGGAGGAGTAGGAGTACAAGCCCAGGTCGTAGAAGAAGCAGAAGTCGTAGTCTGCGGGGAAGGAGGAACCGAAGTCCAGTTAGAGAAGGGAGCGGAGAGAGGCGAGCTAGATTTGAGCAGTGGAACAGGGAAGGAGGACAGCAAGCAAATCATAACAATGCCAATTTTGATGGAAGTAAAAGTAGTCAT

Coding sequence (CDS)

AGAGGTGCTGTAATGGCCTCCATTTCCGGGACGCAGATGGGCAGGGCTAACTGTCCCTTCTACTCCAAGGTCGGGGCCTGCCGCCATGGCGATCAGTGTTCCCGGTTGCACCCCAAGCCTCTCATAAGCCCCACACTCTTGCTTTCTAATATGTACCAGAGACTTGATACGATTAGCCCTGGTGTCGACGCGCGCGGAAATCCGATCGACCCTCGTAAGATCCAGCGCCATTTTGAGGAGTTCTATGAAGATATATTCGAGGAAATGAGCAAGTACGGTGAGATTGAGAGTTTGAATGTCTGTGACAACCTAGGTGACCACATGGTGGGTAATGTTTATGTTCAATATCGAGAGGAGGGGCAAGCCGCAAATGCTCTTCGGAATCTTAGGGACAGATATTATGGTGGACTTCTAATTAGAGTCGACTTCTCTCCAGTGACAGACTTTATAGTAGCCGCTTGTAGACGATACGAAGAGAACATGTGTAATTTTGGTCCCTATTGTAATTTCATGCATCTGAAGAGGATAAGTTGGGAGCTGAGGAGCAGACTGTTTAGGACCAGGAGCCACAGCCCCGATAGGCATCGGAATTATGAACAACGCTCTTATGGTAGACATGGTCATAGTCGGAGGTATGATGACCGAGATGCTTATCATGAAAGCCGGAGCAGGAGGAGTAGGAGTACAAGCCCAGGTCGTAGAAGAAGCAGAAGTCGTAGTCTGCGGGGAAGGAGGAACCGAAGTCCAGTTAGAGAAGGGAGCGGAGAGAGGCGAGCTAGATTTGAGCAGTGGAACAGGGAAGGAGGACAGCAAGCAAATCATAACAATGCCAATTTTGATGGAAGTAAAAGTAGTCAT

Protein sequence

RGAVMASISGTQMGRANCPFYSKVGACRHGDQCSRLHPKPLISPTLLLSNMYQRLDTISPGVDARGNPIDPRKIQRHFEEFYEDIFEEMSKYGEIESLNVCDNLGDHMVGNVYVQYREEGQAANALRNLRDRYYGGLLIRVDFSPVTDFIVAACRRYEENMCNFGPYCNFMHLKRISWELRSRLFRTRSHSPDRHRNYEQRSYGRHGHSRRYDDRDAYHESRSRRSRSTSPGRRRSRSRSLRGRRNRSPVREGSGERRARFEQWNREGGQQANHNNANFDGSKSSH

Homology

[Close]

BLAST of MS006768 vs. NCBI nr
Match: XP_022152942.1 (splicing factor U2af small subunit B-like [Momordica charantia])

HSP 1 Score: 548.9 bits (1413), Expect = 2.6e-152
Identity = 278/292 (95.21%), Postives = 278/292 (95.21%), Query Frame = 0


Query: 5   MASISGTQMGRANCPFYSKVGACRHGDQCSRLHPKPLISPTLLLSNMYQRLDTISPGVDA 64
           MASISGTQMGRANCPFYSKVGACRHGDQCSRLHPKPLISPTLLLSNMYQRLDTISPGVDA
Sbjct: 1   MASISGTQMGRANCPFYSKVGACRHGDQCSRLHPKPLISPTLLLSNMYQRLDTISPGVDA 60

Query: 65  RGNPIDPRKIQRHFEEFYEDIFEEMSKYGEIESLNVCDNLGDHMVGNVYVQYREEGQAAN 124
           RGNPIDPRKIQRHFEEFYEDIFEEMSKYGEIESLNVCDNLGDHMVGNVYVQYREEGQAAN
Sbjct: 61  RGNPIDPRKIQRHFEEFYEDIFEEMSKYGEIESLNVCDNLGDHMVGNVYVQYREEGQAAN 120

Query: 125 ALRNLRDRYYGGLLIRVDFSPVTDFIVAACRRYEENMCNFGPYCNFMHLKRISW------ 184
           AL NLRDRYYGGLLIRVDFS VTDFIVAACRRYEENMCNFGPYCNFMHLKRISW      
Sbjct: 121 ALXNLRDRYYGGLLIRVDFSTVTDFIVAACRRYEENMCNFGPYCNFMHLKRISWYAYFLH 180

Query: 185 ----ELRSRLFRTRSHSPDRHRNYEQRSYGRHGHSRRYDDRDAYHESRSRRSRSTSPGRR 244
                LRSRLFRTRSHS DRHRNYEQRSYGRHGHSRRYDDRDAYHESRSRRSRSTSPGRR
Sbjct: 181 FPFYVLRSRLFRTRSHSXDRHRNYEQRSYGRHGHSRRYDDRDAYHESRSRRSRSTSPGRR 240

Query: 245 RSRSRSLRGRRNRSPVREGSGERRARFEQWNREGGQQANHNNANFDGSKSSH 287
           RSRSRSLRGRRNRSPVREGSGERRARFEQWNREGGQQANHNNANFDGSKSSH
Sbjct: 241 RSRSRSLRGRRNRSPVREGSGERRARFEQWNREGGQQANHNNANFDGSKSSH 292

[Close]

BLAST of MS006768 vs. NCBI nr
Match: XP_023519661.1 (splicing factor U2af small subunit B-like [Cucurbita pepo subsp. pepo] >XP_023519662.1 splicing factor U2af small subunit B-like [Cucurbita pepo subsp. pepo] >XP_023519664.1 splicing factor U2af small subunit B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 423.7 bits (1088), Expect = 1.3e-114
Identity = 217/292 (74.32%), Postives = 243/292 (83.22%), Query Frame = 0


Query: 5   MASISGTQMGRANCPFYSKVGACRHGDQCSRLHPKPLISPTLLLSNMYQRLDTISPGVDA 64
           +ASI GT+  R NCPFY K+GACRHGD+CSRLH KP ISPTLLLSNMYQR D I+PGVDA
Sbjct: 5   LASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITPGVDA 64

Query: 65  RGNPIDPRKIQRHFEEFYEDIFEEMSKYGEIESLNVCDNLGDHMVGNVYVQYREEGQAAN 124
           +GNPIDPRKIQ HFEEFYED+F+E++KYGEIESLNVCDNL DHMVGNVYVQ++EE QAAN
Sbjct: 65  QGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFQEEEQAAN 124

Query: 125 ALRNLRDRYYGGLLIRVDFSPVTDFIVAACRRYEENMCNFGPYCNFMHLKRISWELRSRL 184
           ALRNL  R+Y G  I VDFSPVTDF  A CR+YEENMCN G YCNFMHLKRIS ELR  L
Sbjct: 125 ALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRISRELRHEL 184

Query: 185 F----------RTRSHSPDRHRNYEQRSYGRHGHSRRYDDRDAYHESRSRRSRSTSPGRR 244
           F          R+RS SP RHR+YE+R YGRHGHSRRYD+RDAYH+SR+RR RSTSPG R
Sbjct: 185 FASYHRRRSHSRSRSRSPYRHRSYEERHYGRHGHSRRYDERDAYHDSRNRRHRSTSPGHR 244

Query: 245 RSRSRSLRGRRNRSPVREGSGERRARFEQWNREGGQQANHNNANFDGSKSSH 287
            SRSRS RGR+NRSPVREGS ERRA+ EQWNRE  QQ N+NNAN DG+++SH
Sbjct: 245 GSRSRSPRGRKNRSPVREGSEERRAKIEQWNREKEQQENYNNANSDGNENSH 296

[Close]

BLAST of MS006768 vs. NCBI nr
Match: XP_022964867.1 (splicing factor U2af small subunit B-like [Cucurbita moschata] >XP_022964868.1 splicing factor U2af small subunit B-like [Cucurbita moschata] >XP_022964870.1 splicing factor U2af small subunit B-like [Cucurbita moschata] >KAG7019309.1 Splicing factor U2af small subunit A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 421.8 bits (1083), Expect = 4.8e-114
Identity = 216/292 (73.97%), Postives = 242/292 (82.88%), Query Frame = 0


Query: 5   MASISGTQMGRANCPFYSKVGACRHGDQCSRLHPKPLISPTLLLSNMYQRLDTISPGVDA 64
           +ASI GT+  R NCPFY K+GACRHGD+CSRLH KP ISPTLLLSNMYQR D I+PGVDA
Sbjct: 5   LASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITPGVDA 64

Query: 65  RGNPIDPRKIQRHFEEFYEDIFEEMSKYGEIESLNVCDNLGDHMVGNVYVQYREEGQAAN 124
           +GNPIDPRKIQ HFEEFYED+F+E++KYGEIESLNVCDNL DHMVGNVYVQ++EE QAAN
Sbjct: 65  QGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFQEEEQAAN 124

Query: 125 ALRNLRDRYYGGLLIRVDFSPVTDFIVAACRRYEENMCNFGPYCNFMHLKRISWELRSRL 184
           ALRNL  R+Y G  I VDFSPVTDF  A CR+YEENMCN G YCNFMHLKRIS ELR  L
Sbjct: 125 ALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRISRELRHEL 184

Query: 185 F----------RTRSHSPDRHRNYEQRSYGRHGHSRRYDDRDAYHESRSRRSRSTSPGRR 244
           F          R+RS SP RHR+YE+R YGRHGHSRRYD+RDAYH+SR+RR RSTSPG R
Sbjct: 185 FASYHRRRSHSRSRSRSPYRHRSYEERHYGRHGHSRRYDERDAYHDSRNRRHRSTSPGHR 244

Query: 245 RSRSRSLRGRRNRSPVREGSGERRARFEQWNREGGQQANHNNANFDGSKSSH 287
            SRSRS RGR+NRSPVREGS ERRA+ EQWNRE  QQ N+NN+N DG ++SH
Sbjct: 245 GSRSRSPRGRKNRSPVREGSEERRAKIEQWNREKEQQKNYNNSNSDGHENSH 296

[Close]

BLAST of MS006768 vs. NCBI nr
Match: XP_022970323.1 (splicing factor U2af small subunit B-like [Cucurbita maxima] >XP_022970325.1 splicing factor U2af small subunit B-like [Cucurbita maxima] >XP_022970326.1 splicing factor U2af small subunit B-like [Cucurbita maxima])

HSP 1 Score: 415.2 bits (1066), Expect = 4.5e-112
Identity = 215/292 (73.63%), Postives = 241/292 (82.53%), Query Frame = 0


Query: 5   MASISGTQMGRANCPFYSKVGACRHGDQCSRLHPKPLISPTLLLSNMYQRLDTISPGVDA 64
           +ASI GT+  R NCPFY K+GACRHGD+CSRLH KP ISPTLLLSNMYQR D I+PGVDA
Sbjct: 5   LASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITPGVDA 64

Query: 65  RGNPIDPRKIQRHFEEFYEDIFEEMSKYGEIESLNVCDNLGDHMVGNVYVQYREEGQAAN 124
           +GNPIDPRKIQ HFEEFYED+F+E++KYGEIESLNVCDNL DHMVGNVYVQ++EE QAAN
Sbjct: 65  QGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFQEEEQAAN 124

Query: 125 ALRNLRDRYYGGLLIRVDFSPVTDFIVAACRRYEENMCNFGPYCNFMHLKRISWELRSRL 184
           ALRNL  R+Y G  I VDFSPVTDF  A CR+YEENMCN G YCNFMHLKRIS ELR  L
Sbjct: 125 ALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRISRELRHEL 184

Query: 185 F----------RTRSHSPDRHRNYEQRSYGRHGHSRRYDDRDAYHESRSRRSRSTSPGRR 244
           F          R+RS SP RHR+YE+R YGRHGHSRRYD+RDAYH+SR+RR RSTSPG R
Sbjct: 185 FASYHRRRGHSRSRSRSPYRHRSYEERHYGRHGHSRRYDERDAYHDSRNRRHRSTSPGHR 244

Query: 245 RSRSRSLRGRRNRSPVREGSGERRARFEQWNREGGQQANHNNANFDGSKSSH 287
            SRSRS RGR+NRSPVRE S ERRA+ EQWNRE  +Q N+NNAN DG++ SH
Sbjct: 245 GSRSRSPRGRKNRSPVREESEERRAKIEQWNRE-KEQENYNNANSDGNEHSH 295

[Close]

BLAST of MS006768 vs. NCBI nr
Match: XP_008461981.1 (PREDICTED: splicing factor U2af small subunit B-like [Cucumis melo] >XP_008461988.1 PREDICTED: splicing factor U2af small subunit B-like [Cucumis melo] >KAA0065889.1 splicing factor U2af small subunit B-like [Cucumis melo var. makuwa] >TYK15128.1 splicing factor U2af small subunit B-like [Cucumis melo var. makuwa])

HSP 1 Score: 411.0 bits (1055), Expect = 8.4e-111
Identity = 214/292 (73.29%), Postives = 239/292 (81.85%), Query Frame = 0


Query: 5   MASISGTQMGRANCPFYSKVGACRHGDQCSRLHPKPLISPTLLLSNMYQRLDTISPGVDA 64
           +ASI GT+  R NCPFY K+GACRHGD+CSRLH KP ISPTLLLSNMYQR D I+PGVDA
Sbjct: 5   LASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITPGVDA 64

Query: 65  RGNPIDPRKIQRHFEEFYEDIFEEMSKYGEIESLNVCDNLGDHMVGNVYVQYREEGQAAN 124
           +GNPIDPRKIQ HFEEFYED+F+E++KYGEIESLNVCDNL DHMVGNVYVQ+REE QAAN
Sbjct: 65  QGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEEQAAN 124

Query: 125 ALRNLRDRYYGGLLIRVDFSPVTDFIVAACRRYEENMCNFGPYCNFMHLKRISWELRSRL 184
           ALRNL  R+Y G  I VDFSPVTDF  A CR+YEENMCN G YCNFMHLKRI  ELR  L
Sbjct: 125 ALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGRELRHEL 184

Query: 185 F----------RTRSHSPDRHRNYEQRSYGRHGHSRRYDDRDAYHESRSRRSRSTSPGRR 244
           F          R+RS SP RHR+YE+RSYGRHGHSRRYD+RDAYHE+RSRR R+TSPG  
Sbjct: 185 FAMYRRRRSHSRSRSRSPYRHRSYEERSYGRHGHSRRYDERDAYHENRSRRRRTTSPG-H 244

Query: 245 RSRSRSLRGRRNRSPVREGSGERRARFEQWNREGGQQANHNNANFDGSKSSH 287
           RSRSRS RGR+NRSP REGS ERRA+ EQWNRE  +Q N NNA  D +++SH
Sbjct: 245 RSRSRSPRGRKNRSPAREGSEERRAKIEQWNRE-REQGNDNNAKSDDNRNSH 294

[Close]

BLAST of MS006768 vs. ExPASy Swiss-Prot
Match: Q9ZQW8 (Splicing factor U2af small subunit A OS=Oryza sativa subsp. japonica OX=39947 GN=U2AF35A PE=2 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 4.2e-81
Identity = 168/286 (58.74%), Postives = 194/286 (67.83%), Query Frame = 0


Query: 5   MASISGTQMGRANCPFYSKVGACRHGDQCSRLHPKPLISPTLLLSNMYQRLDTISPGVDA 64
           +ASI GT+  R NCPFY K+GACRHGD+CSRLH KP +SPTLLLSNMY R D I+PG+DA
Sbjct: 5   LASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNKPSVSPTLLLSNMYLRPDMITPGIDA 64

Query: 65  RGNPIDPRKIQRHFEEFYEDIFEEMSKYGEIESLNVCDNLGDHMVGNVYVQYREEGQAAN 124
           +GNPIDP KIQ  FE+FYEDIFEE+SKYGEIESL+VCDN  DHM+GNVYVQ+REE QAA 
Sbjct: 65  QGNPIDPEKIQADFEDFYEDIFEELSKYGEIESLHVCDNFADHMIGNVYVQFREEDQAAR 124

Query: 125 ALRNLRDRYYGGLLIRVDFSPVTDFIVAACRRYEENMCNFGPYCNFMHLKRISWELRSRL 184
           AL+ L  RYY G  I V+FSPV+DF  A CR+YEEN CN G YCNFMH+K I  +LR RL
Sbjct: 125 ALQALTGRYYSGRPIIVEFSPVSDFREATCRQYEENSCNRGGYCNFMHVKEIGRDLRKRL 184

Query: 185 F-----RTRSHSPDRHRN----YEQRSYGRHGHSRRYDDRDAYH--------------ES 244
           F       RSHS  R R+    + +R Y R   SR  D  D Y                 
Sbjct: 185 FGHLHRSRRSHSHGRSRSPSPYHYRRDYDRRSSSRSRDHDDYYRGGSHDYYRGGSRRSSE 244

Query: 245 RSRRSRSTSPGRRRSRSRSLRGRRNRSPVREGSGERRARFEQWNRE 268
           R R S  +   RRR RSR+      RSPVR+GS ERRA+ EQWNRE
Sbjct: 245 RHRSSYDSDGSRRRHRSRT------RSPVRDGSEERRAQIEQWNRE 284

[Close]

BLAST of MS006768 vs. ExPASy Swiss-Prot
Match: Q9S709 (Splicing factor U2af small subunit A OS=Arabidopsis thaliana OX=3702 GN=U2AF35A PE=1 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 1.6e-80
Identity = 164/286 (57.34%), Postives = 195/286 (68.18%), Query Frame = 0


Query: 5   MASISGTQMGRANCPFYSKVGACRHGDQCSRLHPKPLISPTLLLSNMYQRLDTISPGVDA 64
           +ASI GT+  R NCPFY K+GACRHGD+CSRLH +P ISPTLLLSNMYQR D I+PGVDA
Sbjct: 5   LASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITPGVDA 64

Query: 65  RGNPIDPRKIQRHFEEFYEDIFEEMSKYGEIESLNVCDNLGDHMVGNVYVQYREEGQAAN 124
           +G P+DPRKIQ HFE+F+ED+FEE+ K+GEIESLN+CDNL DHM+GNVYVQ++EE QAA 
Sbjct: 65  QGQPLDPRKIQEHFEDFFEDLFEELGKFGEIESLNICDNLADHMIGNVYVQFKEEDQAAA 124

Query: 125 ALRNLRDRYYGGLLIRVDFSPVTDFIVAACRRYEENMCNFGPYCNFMHLKRISWELRSRL 184
           AL+ L+ R+Y G  I  DFSPVTDF  A CR+YEEN CN G YCNFMH+K +S ELR +L
Sbjct: 125 ALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKLVSRELRRKL 184

Query: 185 F---------------RTRSHSPDRHRNYEQRSYGRHGHSRRYDDRDAYHESRSRRSRST 244
           F               R+RS SP   R+ ++R       S R  DR+ Y     +RS   
Sbjct: 185 FGRYRRSYRRGSRSRSRSRSISPRNKRDNDRRDPSHREFSHRDRDREFYRHGSGKRSSER 244

Query: 245 SPGRRRSRSRSLRGRRNRSPV--------REGSGERRARFEQWNRE 268
           S  + R  S   RGRR  SP         REGS ERRAR EQWNRE
Sbjct: 245 SERQERDGS---RGRRQASPKRGGSPGGGREGSEERRARIEQWNRE 287

[Close]

BLAST of MS006768 vs. ExPASy Swiss-Prot
Match: Q9FMY5 (Splicing factor U2af small subunit B OS=Arabidopsis thaliana OX=3702 GN=U2AF35B PE=1 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 3.6e-80
Identity = 165/285 (57.89%), Postives = 196/285 (68.77%), Query Frame = 0


Query: 5   MASISGTQMGRANCPFYSKVGACRHGDQCSRLHPKPLISPTLLLSNMYQRLDTISPGVDA 64
           +ASI GT+  R NCPFY K+GACRHGD+CSRLH +P ISPTLLLSNMYQR D I+PGVD 
Sbjct: 5   LASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITPGVDP 64

Query: 65  RGNPIDPRKIQRHFEEFYEDIFEEMSKYGEIESLNVCDNLGDHMVGNVYVQYREEGQAAN 124
           +G P+DP KIQ HFE+FYEDIFEE++K+GE+ESLNVCDNL DHM+GNVYV ++EE  AA 
Sbjct: 65  QGQPLDPSKIQDHFEDFYEDIFEELNKFGEVESLNVCDNLADHMIGNVYVLFKEEDHAAA 124

Query: 125 ALRNLRDRYYGGLLIRVDFSPVTDFIVAACRRYEENMCNFGPYCNFMHLKRISWELRSRL 184
           AL+ L+ R+Y G  I  DFSPVTDF  A CR+YEEN CN G YCNFMH+K+IS ELR +L
Sbjct: 125 ALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENSCNRGGYCNFMHVKQISRELRRKL 184

Query: 185 F-------------RTRSHSPDRHRNYEQ-------RSYGRHGHSRRYDDRDAYHESRSR 244
           F             R+RS SP R R + +       R   RHG+ +R  DR   H+    
Sbjct: 185 FGRYRRSYRRGSRSRSRSISPRRKREHSRERERGDVRDRDRHGNGKRSSDRSERHD---- 244

Query: 245 RSRSTSPGRRRSRSRSLRGRRNRSP--VREGSGERRARFEQWNRE 268
                  GRRR  S     +R+RSP  VREGS ERRAR EQWNRE
Sbjct: 245 ---RDGGGRRRHGS----PKRSRSPRNVREGSEERRARIEQWNRE 278

[Close]

BLAST of MS006768 vs. ExPASy Swiss-Prot
Match: Q6AUG0 (Splicing factor U2af small subunit B OS=Oryza sativa subsp. japonica OX=39947 GN=U2AF35B PE=2 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 1.5e-73
Identity = 159/299 (53.18%), Postives = 193/299 (64.55%), Query Frame = 0


Query: 5   MASISGTQMGRANCPFYSKVGACRHGDQCSRLHPKPLISPTLLLSNMYQRLDTISPGVDA 64
           +ASI GT+  R NCPFY K+GACRHGD+CSRLH +P +SPT++L+NMYQR D I+PGVDA
Sbjct: 5   LASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTVSPTIVLANMYQRPDMITPGVDA 64

Query: 65  RGNPIDPRKIQRHFEEFYEDIFEEMSKYGEIESLNVCDNLGDHMVGNVYVQYREEGQAAN 124
           +G PIDP K+Q HFE+FYEDI+EE+SK+GE+E+LNVCDNL DHM+GNVYVQ+REE QA  
Sbjct: 65  QGQPIDPEKMQEHFEDFYEDIYEELSKFGEVETLNVCDNLADHMIGNVYVQFREEEQAVA 124

Query: 125 ALRNLRDRYYGGLLIRVDFSPVTDFIVAACRRYEENMCNFGPYCNFMHLKRISWELRSRL 184
           A   L+ R+Y G  I V++SPVTDF  A CR++EEN CN G YCNFMH+K+I  ELR +L
Sbjct: 125 AHNALQGRFYSGRPIIVEYSPVTDFREATCRQFEENSCNRGGYCNFMHVKQIGRELRRKL 184

Query: 185 F---------RTRSHSPDRHRNYEQ-------------------RSYGRHGHSRRYDDRD 244
           +         R+RS SP RHR   +                   R  G  G    Y   D
Sbjct: 185 YGGRSRRSHGRSRSPSP-RHRRGNRDRDDFRRERDGYRGGGDGYRGGGGGGGGDGYRGGD 244

Query: 245 AY--------HESRSRRSRSTSPGRRRSRSRSLRGRRNRSPVREGSGERRARFEQWNRE 268
           +Y            SR  R    GRRR  S     RR RSPVRE S ERRA+ EQWNRE
Sbjct: 245 SYRGGGGGGRRGGGSRYDRYDDGGRRRHGSPP---RRARSPVRESSEERRAKIEQWNRE 299

[Close]

BLAST of MS006768 vs. ExPASy Swiss-Prot
Match: A1A4K8 (Splicing factor U2AF 35 kDa subunit OS=Bos taurus OX=9913 GN=U2AF1 PE=2 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 3.9e-50
Identity = 111/246 (45.12%), Postives = 143/246 (58.13%), Query Frame = 0


Query: 5   MASISGTQMGRANCPFYSKVGACRHGDQCSRLHPKPLISPTLLLSNMYQRLDTISPGVDA 64
           +ASI GT+  + NC FY K+GACRHGD+CSRLH KP  S T+ L N+Y+     S   D 
Sbjct: 5   LASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNKPTFSQTIALLNIYRNPQNSSQSADG 64

Query: 65  RGNPIDPRKIQRHFEEFYEDIFEEM-SKYGEIESLNVCDNLGDHMVGNVYVQYREEGQAA 124
               +   ++Q H++EF+E++F EM  KYGE+E +NVCDNLGDH+VGNVYV++R E  A 
Sbjct: 65  LRCAVSDVEMQEHYDEFFEEVFTEMEEKYGEVEEMNVCDNLGDHLVGNVYVKFRREEDAE 124

Query: 125 NALRNLRDRYYGGLLIRVDFSPVTDFIVAACRRYEENMCNFGPYCNFMHLKRISWELRSR 184
            A+ +L +R++ G  I  + SPVTDF  A CR+YE   C  G +CNFMHLK IS ELR  
Sbjct: 125 KAVIDLNNRWFNGQPIHAELSPVTDFREACCRQYEMGECTRGGFCNFMHLKPISRELRRE 184

Query: 185 LF-----RTRSHSPDRHRNYEQRSYGRHGHSRRYDDRDAYHESRSRRSRSTSPGRRRSRS 244
           L+     + RS S  R R    R  GR G       R+                RRRSR 
Sbjct: 185 LYGRRRKKHRSRSRSRERRSRSRDRGRGGGGGGGGGRER--------------DRRRSRD 236

[Close]

BLAST of MS006768 vs. ExPASy TrEMBL
Match: A0A6J1DHM1 (splicing factor U2af small subunit B-like OS=Momordica charantia OX=3673 GN=LOC111020554 PE=4 SV=1)

HSP 1 Score: 548.9 bits (1413), Expect = 1.3e-152
Identity = 278/292 (95.21%), Postives = 278/292 (95.21%), Query Frame = 0


Query: 5   MASISGTQMGRANCPFYSKVGACRHGDQCSRLHPKPLISPTLLLSNMYQRLDTISPGVDA 64
           MASISGTQMGRANCPFYSKVGACRHGDQCSRLHPKPLISPTLLLSNMYQRLDTISPGVDA
Sbjct: 1   MASISGTQMGRANCPFYSKVGACRHGDQCSRLHPKPLISPTLLLSNMYQRLDTISPGVDA 60

Query: 65  RGNPIDPRKIQRHFEEFYEDIFEEMSKYGEIESLNVCDNLGDHMVGNVYVQYREEGQAAN 124
           RGNPIDPRKIQRHFEEFYEDIFEEMSKYGEIESLNVCDNLGDHMVGNVYVQYREEGQAAN
Sbjct: 61  RGNPIDPRKIQRHFEEFYEDIFEEMSKYGEIESLNVCDNLGDHMVGNVYVQYREEGQAAN 120

Query: 125 ALRNLRDRYYGGLLIRVDFSPVTDFIVAACRRYEENMCNFGPYCNFMHLKRISW------ 184
           AL NLRDRYYGGLLIRVDFS VTDFIVAACRRYEENMCNFGPYCNFMHLKRISW      
Sbjct: 121 ALXNLRDRYYGGLLIRVDFSTVTDFIVAACRRYEENMCNFGPYCNFMHLKRISWYAYFLH 180

Query: 185 ----ELRSRLFRTRSHSPDRHRNYEQRSYGRHGHSRRYDDRDAYHESRSRRSRSTSPGRR 244
                LRSRLFRTRSHS DRHRNYEQRSYGRHGHSRRYDDRDAYHESRSRRSRSTSPGRR
Sbjct: 181 FPFYVLRSRLFRTRSHSXDRHRNYEQRSYGRHGHSRRYDDRDAYHESRSRRSRSTSPGRR 240

Query: 245 RSRSRSLRGRRNRSPVREGSGERRARFEQWNREGGQQANHNNANFDGSKSSH 287
           RSRSRSLRGRRNRSPVREGSGERRARFEQWNREGGQQANHNNANFDGSKSSH
Sbjct: 241 RSRSRSLRGRRNRSPVREGSGERRARFEQWNREGGQQANHNNANFDGSKSSH 292

[Close]

BLAST of MS006768 vs. ExPASy TrEMBL
Match: A0A6J1HM65 (splicing factor U2af small subunit B-like OS=Cucurbita moschata OX=3662 GN=LOC111464850 PE=4 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 2.3e-114
Identity = 216/292 (73.97%), Postives = 242/292 (82.88%), Query Frame = 0


Query: 5   MASISGTQMGRANCPFYSKVGACRHGDQCSRLHPKPLISPTLLLSNMYQRLDTISPGVDA 64
           +ASI GT+  R NCPFY K+GACRHGD+CSRLH KP ISPTLLLSNMYQR D I+PGVDA
Sbjct: 5   LASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITPGVDA 64

Query: 65  RGNPIDPRKIQRHFEEFYEDIFEEMSKYGEIESLNVCDNLGDHMVGNVYVQYREEGQAAN 124
           +GNPIDPRKIQ HFEEFYED+F+E++KYGEIESLNVCDNL DHMVGNVYVQ++EE QAAN
Sbjct: 65  QGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFQEEEQAAN 124

Query: 125 ALRNLRDRYYGGLLIRVDFSPVTDFIVAACRRYEENMCNFGPYCNFMHLKRISWELRSRL 184
           ALRNL  R+Y G  I VDFSPVTDF  A CR+YEENMCN G YCNFMHLKRIS ELR  L
Sbjct: 125 ALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRISRELRHEL 184

Query: 185 F----------RTRSHSPDRHRNYEQRSYGRHGHSRRYDDRDAYHESRSRRSRSTSPGRR 244
           F          R+RS SP RHR+YE+R YGRHGHSRRYD+RDAYH+SR+RR RSTSPG R
Sbjct: 185 FASYHRRRSHSRSRSRSPYRHRSYEERHYGRHGHSRRYDERDAYHDSRNRRHRSTSPGHR 244

Query: 245 RSRSRSLRGRRNRSPVREGSGERRARFEQWNREGGQQANHNNANFDGSKSSH 287
            SRSRS RGR+NRSPVREGS ERRA+ EQWNRE  QQ N+NN+N DG ++SH
Sbjct: 245 GSRSRSPRGRKNRSPVREGSEERRAKIEQWNREKEQQKNYNNSNSDGHENSH 296

[Close]

BLAST of MS006768 vs. ExPASy TrEMBL
Match: A0A6J1I2J7 (splicing factor U2af small subunit B-like OS=Cucurbita maxima OX=3661 GN=LOC111469321 PE=4 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 2.2e-112
Identity = 215/292 (73.63%), Postives = 241/292 (82.53%), Query Frame = 0


Query: 5   MASISGTQMGRANCPFYSKVGACRHGDQCSRLHPKPLISPTLLLSNMYQRLDTISPGVDA 64
           +ASI GT+  R NCPFY K+GACRHGD+CSRLH KP ISPTLLLSNMYQR D I+PGVDA
Sbjct: 5   LASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITPGVDA 64

Query: 65  RGNPIDPRKIQRHFEEFYEDIFEEMSKYGEIESLNVCDNLGDHMVGNVYVQYREEGQAAN 124
           +GNPIDPRKIQ HFEEFYED+F+E++KYGEIESLNVCDNL DHMVGNVYVQ++EE QAAN
Sbjct: 65  QGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFQEEEQAAN 124

Query: 125 ALRNLRDRYYGGLLIRVDFSPVTDFIVAACRRYEENMCNFGPYCNFMHLKRISWELRSRL 184
           ALRNL  R+Y G  I VDFSPVTDF  A CR+YEENMCN G YCNFMHLKRIS ELR  L
Sbjct: 125 ALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRISRELRHEL 184

Query: 185 F----------RTRSHSPDRHRNYEQRSYGRHGHSRRYDDRDAYHESRSRRSRSTSPGRR 244
           F          R+RS SP RHR+YE+R YGRHGHSRRYD+RDAYH+SR+RR RSTSPG R
Sbjct: 185 FASYHRRRGHSRSRSRSPYRHRSYEERHYGRHGHSRRYDERDAYHDSRNRRHRSTSPGHR 244

Query: 245 RSRSRSLRGRRNRSPVREGSGERRARFEQWNREGGQQANHNNANFDGSKSSH 287
            SRSRS RGR+NRSPVRE S ERRA+ EQWNRE  +Q N+NNAN DG++ SH
Sbjct: 245 GSRSRSPRGRKNRSPVREESEERRAKIEQWNRE-KEQENYNNANSDGNEHSH 295

[Close]

BLAST of MS006768 vs. ExPASy TrEMBL
Match: A0A1S3CFU2 (splicing factor U2af small subunit B-like OS=Cucumis melo OX=3656 GN=LOC103500459 PE=4 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 4.1e-111
Identity = 214/292 (73.29%), Postives = 239/292 (81.85%), Query Frame = 0


Query: 5   MASISGTQMGRANCPFYSKVGACRHGDQCSRLHPKPLISPTLLLSNMYQRLDTISPGVDA 64
           +ASI GT+  R NCPFY K+GACRHGD+CSRLH KP ISPTLLLSNMYQR D I+PGVDA
Sbjct: 5   LASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITPGVDA 64

Query: 65  RGNPIDPRKIQRHFEEFYEDIFEEMSKYGEIESLNVCDNLGDHMVGNVYVQYREEGQAAN 124
           +GNPIDPRKIQ HFEEFYED+F+E++KYGEIESLNVCDNL DHMVGNVYVQ+REE QAAN
Sbjct: 65  QGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEEQAAN 124

Query: 125 ALRNLRDRYYGGLLIRVDFSPVTDFIVAACRRYEENMCNFGPYCNFMHLKRISWELRSRL 184
           ALRNL  R+Y G  I VDFSPVTDF  A CR+YEENMCN G YCNFMHLKRI  ELR  L
Sbjct: 125 ALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGRELRHEL 184

Query: 185 F----------RTRSHSPDRHRNYEQRSYGRHGHSRRYDDRDAYHESRSRRSRSTSPGRR 244
           F          R+RS SP RHR+YE+RSYGRHGHSRRYD+RDAYHE+RSRR R+TSPG  
Sbjct: 185 FAMYRRRRSHSRSRSRSPYRHRSYEERSYGRHGHSRRYDERDAYHENRSRRRRTTSPG-H 244

Query: 245 RSRSRSLRGRRNRSPVREGSGERRARFEQWNREGGQQANHNNANFDGSKSSH 287
           RSRSRS RGR+NRSP REGS ERRA+ EQWNRE  +Q N NNA  D +++SH
Sbjct: 245 RSRSRSPRGRKNRSPAREGSEERRAKIEQWNRE-REQGNDNNAKSDDNRNSH 294

[Close]

BLAST of MS006768 vs. ExPASy TrEMBL
Match: A0A5D3CV58 (Splicing factor U2af small subunit B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold259G00620 PE=4 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 4.1e-111
Identity = 214/292 (73.29%), Postives = 239/292 (81.85%), Query Frame = 0


Query: 5   MASISGTQMGRANCPFYSKVGACRHGDQCSRLHPKPLISPTLLLSNMYQRLDTISPGVDA 64
           +ASI GT+  R NCPFY K+GACRHGD+CSRLH KP ISPTLLLSNMYQR D I+PGVDA
Sbjct: 5   LASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHTKPSISPTLLLSNMYQRPDMITPGVDA 64

Query: 65  RGNPIDPRKIQRHFEEFYEDIFEEMSKYGEIESLNVCDNLGDHMVGNVYVQYREEGQAAN 124
           +GNPIDPRKIQ HFEEFYED+F+E++KYGEIESLNVCDNL DHMVGNVYVQ+REE QAAN
Sbjct: 65  QGNPIDPRKIQDHFEEFYEDLFQELNKYGEIESLNVCDNLADHMVGNVYVQFREEEQAAN 124

Query: 125 ALRNLRDRYYGGLLIRVDFSPVTDFIVAACRRYEENMCNFGPYCNFMHLKRISWELRSRL 184
           ALRNL  R+Y G  I VDFSPVTDF  A CR+YEENMCN G YCNFMHLKRI  ELR  L
Sbjct: 125 ALRNLSGRFYAGRPIIVDFSPVTDFREATCRQYEENMCNRGGYCNFMHLKRIGRELRHEL 184

Query: 185 F----------RTRSHSPDRHRNYEQRSYGRHGHSRRYDDRDAYHESRSRRSRSTSPGRR 244
           F          R+RS SP RHR+YE+RSYGRHGHSRRYD+RDAYHE+RSRR R+TSPG  
Sbjct: 185 FAMYRRRRSHSRSRSRSPYRHRSYEERSYGRHGHSRRYDERDAYHENRSRRRRTTSPG-H 244

Query: 245 RSRSRSLRGRRNRSPVREGSGERRARFEQWNREGGQQANHNNANFDGSKSSH 287
           RSRSRS RGR+NRSP REGS ERRA+ EQWNRE  +Q N NNA  D +++SH
Sbjct: 245 RSRSRSPRGRKNRSPAREGSEERRAKIEQWNRE-REQGNDNNAKSDDNRNSH 294

[Close]

BLAST of MS006768 vs. TAIR 10
Match: AT1G27650.1 (U2 snRNP auxiliary factor small subunit, putative )

HSP 1 Score: 300.8 bits (769), Expect = 1.1e-81
Identity = 164/286 (57.34%), Postives = 195/286 (68.18%), Query Frame = 0


Query: 5   MASISGTQMGRANCPFYSKVGACRHGDQCSRLHPKPLISPTLLLSNMYQRLDTISPGVDA 64
           +ASI GT+  R NCPFY K+GACRHGD+CSRLH +P ISPTLLLSNMYQR D I+PGVDA
Sbjct: 5   LASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITPGVDA 64

Query: 65  RGNPIDPRKIQRHFEEFYEDIFEEMSKYGEIESLNVCDNLGDHMVGNVYVQYREEGQAAN 124
           +G P+DPRKIQ HFE+F+ED+FEE+ K+GEIESLN+CDNL DHM+GNVYVQ++EE QAA 
Sbjct: 65  QGQPLDPRKIQEHFEDFFEDLFEELGKFGEIESLNICDNLADHMIGNVYVQFKEEDQAAA 124

Query: 125 ALRNLRDRYYGGLLIRVDFSPVTDFIVAACRRYEENMCNFGPYCNFMHLKRISWELRSRL 184
           AL+ L+ R+Y G  I  DFSPVTDF  A CR+YEEN CN G YCNFMH+K +S ELR +L
Sbjct: 125 ALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNFMHVKLVSRELRRKL 184

Query: 185 F---------------RTRSHSPDRHRNYEQRSYGRHGHSRRYDDRDAYHESRSRRSRST 244
           F               R+RS SP   R+ ++R       S R  DR+ Y     +RS   
Sbjct: 185 FGRYRRSYRRGSRSRSRSRSISPRNKRDNDRRDPSHREFSHRDRDREFYRHGSGKRSSER 244

Query: 245 SPGRRRSRSRSLRGRRNRSPV--------REGSGERRARFEQWNRE 268
           S  + R  S   RGRR  SP         REGS ERRAR EQWNRE
Sbjct: 245 SERQERDGS---RGRRQASPKRGGSPGGGREGSEERRARIEQWNRE 287

[Close]

BLAST of MS006768 vs. TAIR 10
Match: AT5G42820.2 (Zinc finger C-x8-C-x5-C-x3-H type family protein )

HSP 1 Score: 299.7 bits (766), Expect = 2.6e-81
Identity = 165/285 (57.89%), Postives = 196/285 (68.77%), Query Frame = 0


Query: 5   MASISGTQMGRANCPFYSKVGACRHGDQCSRLHPKPLISPTLLLSNMYQRLDTISPGVDA 64
           +ASI GT+  R NCPFY K+GACRHGD+CSRLH +P ISPTLLLSNMYQR D I+PGVD 
Sbjct: 5   LASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITPGVDP 64

Query: 65  RGNPIDPRKIQRHFEEFYEDIFEEMSKYGEIESLNVCDNLGDHMVGNVYVQYREEGQAAN 124
           +G P+DP KIQ HFE+FYEDIFEE++K+GE+ESLNVCDNL DHM+GNVYV ++EE  AA 
Sbjct: 65  QGQPLDPSKIQDHFEDFYEDIFEELNKFGEVESLNVCDNLADHMIGNVYVLFKEEDHAAA 124

Query: 125 ALRNLRDRYYGGLLIRVDFSPVTDFIVAACRRYEENMCNFGPYCNFMHLKRISWELRSRL 184
           AL+ L+ R+Y G  I  DFSPVTDF  A CR+YEEN CN G YCNFMH+K+IS ELR +L
Sbjct: 125 ALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENSCNRGGYCNFMHVKQISRELRRKL 184

Query: 185 F-------------RTRSHSPDRHRNYEQ-------RSYGRHGHSRRYDDRDAYHESRSR 244
           F             R+RS SP R R + +       R   RHG+ +R  DR   H+    
Sbjct: 185 FGRYRRSYRRGSRSRSRSISPRRKREHSRERERGDVRDRDRHGNGKRSSDRSERHD---- 244

Query: 245 RSRSTSPGRRRSRSRSLRGRRNRSP--VREGSGERRARFEQWNRE 268
                  GRRR  S     +R+RSP  VREGS ERRAR EQWNRE
Sbjct: 245 ---RDGGGRRRHGS----PKRSRSPRNVREGSEERRARIEQWNRE 278

[Close]

BLAST of MS006768 vs. TAIR 10
Match: AT5G42820.1 (Zinc finger C-x8-C-x5-C-x3-H type family protein )

HSP 1 Score: 299.7 bits (766), Expect = 2.6e-81
Identity = 165/285 (57.89%), Postives = 196/285 (68.77%), Query Frame = 0


Query: 5   MASISGTQMGRANCPFYSKVGACRHGDQCSRLHPKPLISPTLLLSNMYQRLDTISPGVDA 64
           +ASI GT+  R NCPFY K+GACRHGD+CSRLH +P ISPTLLLSNMYQR D I+PGVD 
Sbjct: 5   LASIFGTEKDRVNCPFYFKIGACRHGDRCSRLHNRPTISPTLLLSNMYQRPDMITPGVDP 64

Query: 65  RGNPIDPRKIQRHFEEFYEDIFEEMSKYGEIESLNVCDNLGDHMVGNVYVQYREEGQAAN 124
           +G P+DP KIQ HFE+FYEDIFEE++K+GE+ESLNVCDNL DHM+GNVYV ++EE  AA 
Sbjct: 65  QGQPLDPSKIQDHFEDFYEDIFEELNKFGEVESLNVCDNLADHMIGNVYVLFKEEDHAAA 124

Query: 125 ALRNLRDRYYGGLLIRVDFSPVTDFIVAACRRYEENMCNFGPYCNFMHLKRISWELRSRL 184
           AL+ L+ R+Y G  I  DFSPVTDF  A CR+YEEN CN G YCNFMH+K+IS ELR +L
Sbjct: 125 ALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENSCNRGGYCNFMHVKQISRELRRKL 184

Query: 185 F-------------RTRSHSPDRHRNYEQ-------RSYGRHGHSRRYDDRDAYHESRSR 244
           F             R+RS SP R R + +       R   RHG+ +R  DR   H+    
Sbjct: 185 FGRYRRSYRRGSRSRSRSISPRRKREHSRERERGDVRDRDRHGNGKRSSDRSERHD---- 244

Query: 245 RSRSTSPGRRRSRSRSLRGRRNRSP--VREGSGERRARFEQWNRE 268
                  GRRR  S     +R+RSP  VREGS ERRAR EQWNRE
Sbjct: 245 ---RDGGGRRRHGS----PKRSRSPRNVREGSEERRARIEQWNRE 278

[Close]

BLAST of MS006768 vs. TAIR 10
Match: AT1G27650.2 (U2 snRNP auxiliary factor small subunit, putative )

HSP 1 Score: 226.1 bits (575), Expect = 3.6e-59
Identity = 130/240 (54.17%), Postives = 156/240 (65.00%), Query Frame = 0


Query: 51  MYQRLDTISPGVDARGNPIDPRKIQRHFEEFYEDIFEEMSKYGEIESLNVCDNLGDHMVG 110
           MYQR D I+PGVDA+G P+DPRKIQ HFE+F+ED+FEE+ K+GEIESLN+CDNL DHM+G
Sbjct: 1   MYQRPDMITPGVDAQGQPLDPRKIQEHFEDFFEDLFEELGKFGEIESLNICDNLADHMIG 60

Query: 111 NVYVQYREEGQAANALRNLRDRYYGGLLIRVDFSPVTDFIVAACRRYEENMCNFGPYCNF 170
           NVYVQ++EE QAA AL+ L+ R+Y G  I  DFSPVTDF  A CR+YEEN CN G YCNF
Sbjct: 61  NVYVQFKEEDQAAAALQALQGRFYSGRPIIADFSPVTDFREATCRQYEENNCNRGGYCNF 120

Query: 171 MHLKRISWELRSRLF---------------RTRSHSPDRHRNYEQRSYGRHGHSRRYDDR 230
           MH+K +S ELR +LF               R+RS SP   R+ ++R       S R  DR
Sbjct: 121 MHVKLVSRELRRKLFGRYRRSYRRGSRSRSRSRSISPRNKRDNDRRDPSHREFSHRDRDR 180

Query: 231 DAYHESRSRRSRSTSPGRRRSRSRSLRGRRNRSPV--------REGSGERRARFEQWNRE 268
           + Y     +RS   S  + R  S   RGRR  SP         REGS ERRAR EQWNRE
Sbjct: 181 EFYRHGSGKRSSERSERQERDGS---RGRRQASPKRGGSPGGGREGSEERRARIEQWNRE 237

[Close]

BLAST of MS006768 vs. TAIR 10
Match: AT1G10320.1 (Zinc finger C-x8-C-x5-C-x3-H type family protein )

HSP 1 Score: 104.0 bits (258), Expect = 2.1e-22
Identity = 90/292 (30.82%), Postives = 133/292 (45.55%), Query Frame = 0


Query: 10  GTQMGRANCPFYSKVGACRHGDQCSRLHPKPLISPTLLLSNMYQRLDTISPGV---DARG 69
           GT+  +A+CPF+ K GACR G +CSR+H  P  S TLL+ NMY       PG+      G
Sbjct: 238 GTEQDKAHCPFHLKTGACRFGQRCSRVHFYPNKSCTLLMKNMYN-----GPGITWEQDEG 297

Query: 70  NPIDPRKIQRHFEEFYEDIFEEMSKYGEIESLNVCDNLGDHMVGNVYVQYREEGQAANAL 129
                 + +  +EEFYED+  E  KYGE+ +  VC N   H+ GNVYV YR    A  A 
Sbjct: 298 LEYTDEEAELCYEEFYEDVHTEFLKYGELVNFKVCRNGSFHLKGNVYVHYRSLESAILAY 357

Query: 130 RNLRDRYYGGLLIRVDFSPVTDFIVAACRRYEEN---MCNFGPYCNFMHLKR-----ISW 189
           +++  RY+ G  +  +F  ++ + VA C  Y ++    C+ G  CNF+H  R       W
Sbjct: 358 QSINGRYFAGKQVNCEFVNISRWKVAICGEYMKSRLKTCSRGSACNFIHCFRNPGGDYEW 417

Query: 190 ELRSR---------LFRTRSHSPDRHRNYEQRSYGRHGHSRRYDDRDAYHESRSRRSRST 249
               R         +     +S ++H  +E  S G    S      D+ H   SRRSRS 
Sbjct: 418 ADHDRPPPRFWIHKMTSLFGYSDEKHMEHE--SSGSLNDSISDLSTDS-HRQPSRRSRSR 477

Query: 250 SPGRRRSRSR-SLRGRRNRSPVREGSGERRARFEQWNREGGQQANHNNANFD 281
                   S  S R R+     ++ + E + R    N   G  +   + + +
Sbjct: 478 DHDHANVGSTPSYRSRKYHGDTQDSTREDKLRRHAENCHDGDDSPSRDGSLE 521

The following BLAST results are available for this feature:

BLAST of MS006768 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022152942.1	2.6e-152	95.21	splicing factor U2af small subunit B-like [Momordica charantia]	[more]
XP_023519661.1	1.3e-114	74.32	splicing factor U2af small subunit B-like [Cucurbita pepo subsp. pepo] >XP_02351...	[more]
XP_022964867.1	4.8e-114	73.97	splicing factor U2af small subunit B-like [Cucurbita moschata] >XP_022964868.1 s...	[more]
XP_022970323.1	4.5e-112	73.63	splicing factor U2af small subunit B-like [Cucurbita maxima] >XP_022970325.1 spl...	[more]
XP_008461981.1	8.4e-111	73.29	PREDICTED: splicing factor U2af small subunit B-like [Cucumis melo] >XP_00846198...	[more]

BLAST of MS006768 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZQW8	4.2e-81	58.74	Splicing factor U2af small subunit A OS=Oryza sativa subsp. japonica OX=39947 GN...	[more]
Q9S709	1.6e-80	57.34	Splicing factor U2af small subunit A OS=Arabidopsis thaliana OX=3702 GN=U2AF35A ...	[more]
Q9FMY5	3.6e-80	57.89	Splicing factor U2af small subunit B OS=Arabidopsis thaliana OX=3702 GN=U2AF35B ...	[more]
Q6AUG0	1.5e-73	53.18	Splicing factor U2af small subunit B OS=Oryza sativa subsp. japonica OX=39947 GN...	[more]
A1A4K8	3.9e-50	45.12	Splicing factor U2AF 35 kDa subunit OS=Bos taurus OX=9913 GN=U2AF1 PE=2 SV=1	[more]

BLAST of MS006768 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DHM1	1.3e-152	95.21	splicing factor U2af small subunit B-like OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A6J1HM65	2.3e-114	73.97	splicing factor U2af small subunit B-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1I2J7	2.2e-112	73.63	splicing factor U2af small subunit B-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A1S3CFU2	4.1e-111	73.29	splicing factor U2af small subunit B-like OS=Cucumis melo OX=3656 GN=LOC10350045...	[more]
A0A5D3CV58	4.1e-111	73.29	Splicing factor U2af small subunit B-like OS=Cucumis melo var. makuwa OX=1194695...	[more]

BLAST of MS006768 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G27650.1	1.1e-81	57.34	U2 snRNP auxiliary factor small subunit, putative	[more]
AT5G42820.2	2.6e-81	57.89	Zinc finger C-x8-C-x5-C-x3-H type family protein	[more]
AT5G42820.1	2.6e-81	57.89	Zinc finger C-x8-C-x5-C-x3-H type family protein	[more]
AT1G27650.2	3.6e-59	54.17	U2 snRNP auxiliary factor small subunit, putative	[more]
AT1G10320.1	2.1e-22	30.82	Zinc finger C-x8-C-x5-C-x3-H type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009145	U2 auxiliary factor small subunit	PRINTS	PR01848	U2AUXFACTOR	coord: 37..57 score: 50.0 coord: 103..125 score: 60.67 coord: 75..90 score: 52.84 coord: 130..154 score: 44.18 coord: 18..37 score: 76.82 coord: 165..177 score: 61.89
IPR009145	U2 auxiliary factor small subunit	PANTHER	PTHR12620	U2 SNRNP AUXILIARY FACTOR, SMALL SUBUNIT	coord: 5..282
IPR000571	Zinc finger, CCCH-type	SMART	SM00356	c3hfinal6	coord: 148..174 e-value: 0.18 score: 20.9 coord: 13..39 e-value: 0.0034 score: 26.6
IPR000571	Zinc finger, CCCH-type	PFAM	PF00642	zf-CCCH	coord: 14..39 e-value: 7.4E-7 score: 28.9
IPR000571	Zinc finger, CCCH-type	PROSITE	PS50103	ZF_C3H1	coord: 12..40 score: 13.779069
IPR000571	Zinc finger, CCCH-type	PROSITE	PS50103	ZF_C3H1	coord: 148..175 score: 10.492884
IPR003954	RNA recognition motif domain, eukaryote	SMART	SM00361	rrm2_1	coord: 73..142 e-value: 9.8E-5 score: 31.7
IPR012677	Nucleotide-binding alpha-beta plait domain superfamily	GENE3D	3.30.70.330		coord: 43..145 e-value: 4.1E-26 score: 93.3
IPR000504	RNA recognition motif domain	PFAM	PF00076	RRM_1	coord: 83..138 e-value: 2.3E-4 score: 21.0
IPR000504	RNA recognition motif domain	PROSITE	PS50102	RRM	coord: 44..146 score: 9.490363
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 245..264
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 228..244
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 190..227
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 190..286
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 266..286
None	No IPR available	PANTHER	PTHR12620:SF39	SPLICING FACTOR U2AF SMALL SUBUNIT A	coord: 5..282
IPR035979	RNA-binding domain superfamily	SUPERFAMILY	54928	RNA-binding domain, RBD	coord: 32..149

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS006768.1	MS006768.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000398	mRNA splicing, via spliceosome
cellular_component	GO:0089701	U2AF complex
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0003723	RNA binding