MS006431 (gene) Bitter gourd (TR) v1

Overview
NameMS006431
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionphosphoglycerate kinase
Locationscaffold327: 597681 .. 599903 (+)
RNA-Seq ExpressionMS006431
SyntenyMS006431
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTGTTATGGCTACCAAAAGGAGCGTTGGTACTCTGAAGGAGGCTGATTTGAAGGGAAAGAGGGTGTTCGTGAGGGTCGATCTCAATGTTCCTTTGGATGACAACTTCAACATCACCGATGACACCAGAATCCGTGCTGCTGTGCCTACCATTAAGTATTTACTCGGCCATGGTGCCAGGGTCATCCTCTCCAGCCACTTGGTGCGCTACTTCTCCTCTTTCCCTCTTTCGCATTCTCGCAAGATCTATCTATTTGGCATGTTCTCCCGAGTTCTACTTTGGGTTTGCTTCGATCTTTGCATTTTACAGCATGCCCGACAATCGTAACTTTCGATCCTTTGTTTTCAGGAAAGGCCATCATCCCTCTCCCCATTTTTATCATCTTTTTTGGTGGATTTAGATAAACTTGAAAAGGAATATTGCGATTTTGGATAATGTACAATTCGATTGTATTGTCATACGTCATATGCACGATTCAGCTTTTTGGATCTAATATTCATTATGCTCAATATTGGAAAATTCATTGACATGCAGTGTAAAGATCAACGTTGATTATTGTTAGTTAAATTTTCAGTGATCTGCGCCTTTCTTCTTCCGTAAACTTTGGAGTGACGCTGCGTATTTGATTGCGATCGATTAAGATTATGATCTTTACTGAACTGAGGTCGCTTATTGTGTTCAATTTCATGTTAGCATCACTGTTCGATTTGTTTTCAAATTTGCATTATTTTTCATTTTGTCTATTCCCTGGGCTCATTTTGTTTTTTATTATTGTGGTCTTTCAGGGGCGCCCAAAGGGCGTAACGCCGAAATACAGTTTGAAACCTCTTGTTCCCAGGCTTTCTGAGCTCCTCGGAGTTCAGGTATGGCGCCTAAATTTCGTAGAAATATTTGTTTGGGATTTGTGTTTATATGTGATGACAACGTTATCCCTTACTAAAATTTTGTCCCCTCCCCCCACAATAGGTGAAAATGGCCAATGATTCTATTGGCGAGGAAGTTGAGAAGCTGGTCGCTGAATTGCCTGAGGGAGGTGTATTGCTGCTTGAAAATGTGAGGTTTTACAAGGAGGAAGAGAAGAATGACCCCGAGTTTGCCAAGAAGCTGGCTTCCCTCGCGGATCTCTATGTTAACGATGCCTTTGGAACTGCTCACAGGGCTCACGCTTCCACAGAGGGTGTGGCTAAGTTCTTGAAACCGTCTGTTGCTGGTTTCCTTATGCAGAAGGTTTGTTGCACGCTCTATGGCCTTTCTTGTTGTGCGTCAAAATGGACATTAACTCTCTAAACGTCAATGGACATTAACTCTCTAACCGTCATGTTTGTTCATTAGTTTTTAGAAGATGACTGATTGCAACTGATGATTCCTTTGGTTCAGGAACTTGACTACCTCGTTGGAGCTGTGGCTAATCCCAAGAGGCCATTTGCTGCCATTGTTGGTGGTTCAAAGGTGTCTTCAAAGATTGGAGTGATTGAATCCTTGTTGGAGAAGGTCAACCTTCTTGTCCTGGGTGGAGGAATGATCTTTACCTTCTACAAGGCGCAAGGTCACTCAGTGGGGTCATCGCTTGTTGAAGAAGACAAGCTTGGCCTTGCTACTTCGCTCATTGAGAAGGCAAAATCCAAGGGGGTGTCTCTTTTGCTTCCAACCGATGTAGTCATAGCAGATAAATTTGCTGCTGATGCTAACAGCAAGGTTCGTAGTTTTGCTTTTTCCAATTGAATATAGCTTTAACATTCATGGAGAGTAGTTGGTAAGAATTTTATCCATAATTTCAGACTGTGGCCGCATCAAGTATCCCAGATGGTTGGATGGGGTTGGATATTGGACCTGATTCTATTAAGAGCTTCAGTGAAGCATTGGACTCTACCCAGACCATTATTTGGAATGGGCCTATGGGAGTGTTTGAGTTTGAGAAGTTTGCGGCTGGAACGGAGGTGTGAACATTGGACCAGCTATTTGTTATATATCGATGTTCTTCTGTGGCATGTGTATGTACTCAGTGTTGGGTTGATTTACGCTCATGTGCAGGCAATTGCAAGGAAGTTGGCAGAGCTGAGTGGGAAGGGGGTGACCACCATAATTGGAGGAGGCGACTCTGTTGCGGCTGTTGAGAAGGTTGGGCTTGCGGATAAGATGAGCCACATCTCCACTGGAGGCGGTGCCAGCTTAGAGCTTCTTGAAGGAAAACCGCTTCCCGGGGTCCTTGCTCTCGATGATGCT

mRNA sequence

ATTGTTATGGCTACCAAAAGGAGCGTTGGTACTCTGAAGGAGGCTGATTTGAAGGGAAAGAGGGTGTTCGTGAGGGTCGATCTCAATGTTCCTTTGGATGACAACTTCAACATCACCGATGACACCAGAATCCGTGCTGCTGTGCCTACCATTAAGTATTTACTCGGCCATGGTGCCAGGGTCATCCTCTCCAGCCACTTGGGGCGCCCAAAGGGCGTAACGCCGAAATACAGTTTGAAACCTCTTGTTCCCAGGCTTTCTGAGCTCCTCGGAGTTCAGGTGAAAATGGCCAATGATTCTATTGGCGAGGAAGTTGAGAAGCTGGTCGCTGAATTGCCTGAGGGAGGTGTATTGCTGCTTGAAAATGTGAGGTTTTACAAGGAGGAAGAGAAGAATGACCCCGAGTTTGCCAAGAAGCTGGCTTCCCTCGCGGATCTCTATGTTAACGATGCCTTTGGAACTGCTCACAGGGCTCACGCTTCCACAGAGGGTGTGGCTAAGTTCTTGAAACCGTCTGTTGCTGGTTTCCTTATGCAGAAGGAACTTGACTACCTCGTTGGAGCTGTGGCTAATCCCAAGAGGCCATTTGCTGCCATTGTTGGTGGTTCAAAGGTGTCTTCAAAGATTGGAGTGATTGAATCCTTGTTGGAGAAGGTCAACCTTCTTGTCCTGGGTGGAGGAATGATCTTTACCTTCTACAAGGCGCAAGGTCACTCAGTGGGGTCATCGCTTGTTGAAGAAGACAAGCTTGGCCTTGCTACTTCGCTCATTGAGAAGGCAAAATCCAAGGGGGTGTCTCTTTTGCTTCCAACCGATGTAGTCATAGCAGATAAATTTGCTGCTGATGCTAACAGCAAGACTGTGGCCGCATCAAGTATCCCAGATGGTTGGATGGGGTTGGATATTGGACCTGATTCTATTAAGAGCTTCAGTGAAGCATTGGACTCTACCCAGACCATTATTTGGAATGGGCCTATGGGAGTGTTTGAGTTTGAGAAGTTTGCGGCTGGAACGGAGGCAATTGCAAGGAAGTTGGCAGAGCTGAGTGGGAAGGGGGTGACCACCATAATTGGAGGAGGCGACTCTGTTGCGGCTGTTGAGAAGGTTGGGCTTGCGGATAAGATGAGCCACATCTCCACTGGAGGCGGTGCCAGCTTAGAGCTTCTTGAAGGAAAACCGCTTCCCGGGGTCCTTGCTCTCGATGATGCT

Coding sequence (CDS)

ATTGTTATGGCTACCAAAAGGAGCGTTGGTACTCTGAAGGAGGCTGATTTGAAGGGAAAGAGGGTGTTCGTGAGGGTCGATCTCAATGTTCCTTTGGATGACAACTTCAACATCACCGATGACACCAGAATCCGTGCTGCTGTGCCTACCATTAAGTATTTACTCGGCCATGGTGCCAGGGTCATCCTCTCCAGCCACTTGGGGCGCCCAAAGGGCGTAACGCCGAAATACAGTTTGAAACCTCTTGTTCCCAGGCTTTCTGAGCTCCTCGGAGTTCAGGTGAAAATGGCCAATGATTCTATTGGCGAGGAAGTTGAGAAGCTGGTCGCTGAATTGCCTGAGGGAGGTGTATTGCTGCTTGAAAATGTGAGGTTTTACAAGGAGGAAGAGAAGAATGACCCCGAGTTTGCCAAGAAGCTGGCTTCCCTCGCGGATCTCTATGTTAACGATGCCTTTGGAACTGCTCACAGGGCTCACGCTTCCACAGAGGGTGTGGCTAAGTTCTTGAAACCGTCTGTTGCTGGTTTCCTTATGCAGAAGGAACTTGACTACCTCGTTGGAGCTGTGGCTAATCCCAAGAGGCCATTTGCTGCCATTGTTGGTGGTTCAAAGGTGTCTTCAAAGATTGGAGTGATTGAATCCTTGTTGGAGAAGGTCAACCTTCTTGTCCTGGGTGGAGGAATGATCTTTACCTTCTACAAGGCGCAAGGTCACTCAGTGGGGTCATCGCTTGTTGAAGAAGACAAGCTTGGCCTTGCTACTTCGCTCATTGAGAAGGCAAAATCCAAGGGGGTGTCTCTTTTGCTTCCAACCGATGTAGTCATAGCAGATAAATTTGCTGCTGATGCTAACAGCAAGACTGTGGCCGCATCAAGTATCCCAGATGGTTGGATGGGGTTGGATATTGGACCTGATTCTATTAAGAGCTTCAGTGAAGCATTGGACTCTACCCAGACCATTATTTGGAATGGGCCTATGGGAGTGTTTGAGTTTGAGAAGTTTGCGGCTGGAACGGAGGCAATTGCAAGGAAGTTGGCAGAGCTGAGTGGGAAGGGGGTGACCACCATAATTGGAGGAGGCGACTCTGTTGCGGCTGTTGAGAAGGTTGGGCTTGCGGATAAGATGAGCCACATCTCCACTGGAGGCGGTGCCAGCTTAGAGCTTCTTGAAGGAAAACCGCTTCCCGGGGTCCTTGCTCTCGATGATGCT

Protein sequence

IVMATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVILSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLENVRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKELDYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGSSLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDIGPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDSVAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA
Homology
BLAST of MS006431 vs. NCBI nr
Match: XP_022147931.1 (phosphoglycerate kinase, cytosolic [Momordica charantia])

HSP 1 Score: 771.2 bits (1990), Expect = 4.5e-219
Identity = 400/401 (99.75%), Postives = 401/401 (100.00%), Query Frame = 0

Query: 3   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 62
           MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI
Sbjct: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 60

Query: 63  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 122
           LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN
Sbjct: 61  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 120

Query: 123 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKEL 182
           VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFL+PSVAGFLMQKEL
Sbjct: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLEPSVAGFLMQKEL 180

Query: 183 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 242
           DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS
Sbjct: 181 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 240

Query: 243 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 302
           SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI
Sbjct: 241 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 300

Query: 303 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 362
           GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS
Sbjct: 301 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360

Query: 363 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 404
           VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA
Sbjct: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 401

BLAST of MS006431 vs. NCBI nr
Match: XP_023529234.1 (phosphoglycerate kinase, cytosolic [Cucurbita pepo subsp. pepo] >XP_023529235.1 phosphoglycerate kinase, cytosolic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 747.7 bits (1929), Expect = 5.4e-212
Identity = 385/401 (96.01%), Postives = 395/401 (98.50%), Query Frame = 0

Query: 3   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 62
           MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTI+YLLGHGARVI
Sbjct: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIQYLLGHGARVI 60

Query: 63  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 122
           LS+HLGRPKGVTPKYSLKPL+PRLSELLG+QV MAND IGEEVEKLVAEL EGGVLLLEN
Sbjct: 61  LSTHLGRPKGVTPKYSLKPLIPRLSELLGLQVIMANDCIGEEVEKLVAELSEGGVLLLEN 120

Query: 123 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKEL 182
           VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAK+LKPSVAGFLMQKEL
Sbjct: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKYLKPSVAGFLMQKEL 180

Query: 183 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 242
           DYLVGAV+NPKRPFAAIVGGSKVSSKIGVIESLLEKVNLL LGGGMIFTFYKAQGHSVGS
Sbjct: 181 DYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLFLGGGMIFTFYKAQGHSVGS 240

Query: 243 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 302
           SLVEEDKL LATSLIEKAK+KGVSLLLPTDVVIADKFAADANSK VAAS+IPDGWMGLDI
Sbjct: 241 SLVEEDKLDLATSLIEKAKAKGVSLLLPTDVVIADKFAADANSKVVAASAIPDGWMGLDI 300

Query: 303 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 362
           GPDSIKSFSEALDSTQT+IWNGPMGVFEF+KFAAGTEAIARKLAELSGKGVTTIIGGGDS
Sbjct: 301 GPDSIKSFSEALDSTQTVIWNGPMGVFEFDKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360

Query: 363 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 404
           VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA
Sbjct: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 401

BLAST of MS006431 vs. NCBI nr
Match: XP_022989221.1 (phosphoglycerate kinase, cytosolic [Cucurbita maxima] >XP_022989222.1 phosphoglycerate kinase, cytosolic [Cucurbita maxima])

HSP 1 Score: 746.9 bits (1927), Expect = 9.1e-212
Identity = 384/401 (95.76%), Postives = 395/401 (98.50%), Query Frame = 0

Query: 3   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 62
           MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTI+YLLGHGARVI
Sbjct: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIQYLLGHGARVI 60

Query: 63  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 122
           LS+HLGRPKGVTPKYSLKPL+PRLSELLG+QV MAND IGEEVEKLVAEL EGGVLLLEN
Sbjct: 61  LSTHLGRPKGVTPKYSLKPLIPRLSELLGLQVIMANDCIGEEVEKLVAELSEGGVLLLEN 120

Query: 123 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKEL 182
           VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAK+LKPSVAGFLMQKEL
Sbjct: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKYLKPSVAGFLMQKEL 180

Query: 183 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 242
           DYLVGAV+NPKRPFAAIVGGSKVSSKIGVIESLLEKVNLL LGGGMIFTFYKAQGHSVGS
Sbjct: 181 DYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLFLGGGMIFTFYKAQGHSVGS 240

Query: 243 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 302
           SLVEEDKL LATS+IEKAK+KGVSLLLPTDVVIADKFAADANSK VAAS+IPDGWMGLDI
Sbjct: 241 SLVEEDKLDLATSIIEKAKAKGVSLLLPTDVVIADKFAADANSKVVAASAIPDGWMGLDI 300

Query: 303 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 362
           GPDSIKSFSEALDSTQT+IWNGPMGVFEF+KFAAGTEAIARKLAELSGKGVTTIIGGGDS
Sbjct: 301 GPDSIKSFSEALDSTQTVIWNGPMGVFEFDKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360

Query: 363 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 404
           VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA
Sbjct: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 401

BLAST of MS006431 vs. NCBI nr
Match: KAG7023014.1 (hypothetical protein SDJN02_16750 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 746.5 bits (1926), Expect = 1.2e-211
Identity = 384/401 (95.76%), Postives = 395/401 (98.50%), Query Frame = 0

Query: 3   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 62
           MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTI+YLLGHGARVI
Sbjct: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIQYLLGHGARVI 60

Query: 63  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 122
           LS+HLGRPKGVTPKYSLKPL+PRLSELLG+QV MAND IGEEVEKLVAEL EGGVLLLEN
Sbjct: 61  LSTHLGRPKGVTPKYSLKPLIPRLSELLGLQVIMANDCIGEEVEKLVAELSEGGVLLLEN 120

Query: 123 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKEL 182
           VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAK+LKPSVAGFLMQKEL
Sbjct: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKYLKPSVAGFLMQKEL 180

Query: 183 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 242
           DYLVGAV+NPKRPFAAIVGGSKVSSKIGVIESLLEKVNLL LGGGMIFTFYKAQGHSVGS
Sbjct: 181 DYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLFLGGGMIFTFYKAQGHSVGS 240

Query: 243 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 302
           SLVEEDKL LATSL+EKAK+KGVSLLLPTDVVIADKFAADANSK VAAS+IPDGWMGLDI
Sbjct: 241 SLVEEDKLDLATSLMEKAKAKGVSLLLPTDVVIADKFAADANSKVVAASAIPDGWMGLDI 300

Query: 303 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 362
           GPDSIKSFSEALDSTQT+IWNGPMGVFEF+KFAAGTEAIARKLAELSGKGVTTIIGGGDS
Sbjct: 301 GPDSIKSFSEALDSTQTVIWNGPMGVFEFDKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360

Query: 363 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 404
           VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA
Sbjct: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 401

BLAST of MS006431 vs. NCBI nr
Match: KAG6589315.1 (hypothetical protein SDJN03_17880, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 745.0 bits (1922), Expect = 3.5e-211
Identity = 383/401 (95.51%), Postives = 394/401 (98.25%), Query Frame = 0

Query: 3   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 62
           MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTI+YLLGHGARVI
Sbjct: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIQYLLGHGARVI 60

Query: 63  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 122
           LS+HLGRPKGVTPKYSLKPL+PRLSELLG+QV MAND IGEEVEKLVAEL EGGVLLLEN
Sbjct: 61  LSTHLGRPKGVTPKYSLKPLIPRLSELLGLQVIMANDCIGEEVEKLVAELSEGGVLLLEN 120

Query: 123 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKEL 182
           VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAK+LKPSVAGFLMQKEL
Sbjct: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKYLKPSVAGFLMQKEL 180

Query: 183 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 242
           DYLVGAV+NPKRPFAAIVGGSKVSSKIGVIESLLEKVNLL LGGGMIFTFYKAQGHSVGS
Sbjct: 181 DYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLFLGGGMIFTFYKAQGHSVGS 240

Query: 243 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 302
           SLVEEDKL LATSL+EKAK+KGVSLLLPTDVVIADKFAADANSK VAAS+IPDGWMGLDI
Sbjct: 241 SLVEEDKLDLATSLMEKAKAKGVSLLLPTDVVIADKFAADANSKVVAASAIPDGWMGLDI 300

Query: 303 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 362
           GPDSIK FSEALDSTQT+IWNGPMGVFEF+KFAAGTEAIARKLAELSGKGVTTIIGGGDS
Sbjct: 301 GPDSIKGFSEALDSTQTVIWNGPMGVFEFDKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360

Query: 363 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 404
           VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA
Sbjct: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 401

BLAST of MS006431 vs. ExPASy Swiss-Prot
Match: Q42962 (Phosphoglycerate kinase, cytosolic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 1.7e-205
Identity = 363/401 (90.52%), Postives = 391/401 (97.51%), Query Frame = 0

Query: 3   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 62
           MA K+SVG+LKEADLKGKRVFVRVDLNVPLD+NFNITDDTRIRAAVPTIKYL+ HG+ VI
Sbjct: 1   MAVKKSVGSLKEADLKGKRVFVRVDLNVPLDENFNITDDTRIRAAVPTIKYLMQHGSHVI 60

Query: 63  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 122
           L+SHLGRPKGVTPKYSLKPLVPRLSELLGV+VK+ANDSIG EVEKLVAE+PEGGVLLLEN
Sbjct: 61  LASHLGRPKGVTPKYSLKPLVPRLSELLGVEVKIANDSIGPEVEKLVAEIPEGGVLLLEN 120

Query: 123 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKEL 182
           VRFYKEEEKN+PEFAKKLASLADLYVNDAFGTAHRAHASTEGVAK+LKP+VAGFLMQKEL
Sbjct: 121 VRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKYLKPAVAGFLMQKEL 180

Query: 183 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 242
           DYLVGAVANP++PFAAIVGGSKVSSKIGVIESLLEKV++L+LGGGMIFTFYKAQG++VGS
Sbjct: 181 DYLVGAVANPQKPFAAIVGGSKVSSKIGVIESLLEKVDVLLLGGGMIFTFYKAQGYAVGS 240

Query: 243 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 302
           SLVEEDKL LATSL+EKAK+KGVSLLLPTDVVIADKFAADANSK V AS IPDGWMGLDI
Sbjct: 241 SLVEEDKLDLATSLMEKAKAKGVSLLLPTDVVIADKFAADANSKVVPASEIPDGWMGLDI 300

Query: 303 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 362
           GPD+IKSF  ALD+T+T+IWNGPMGVFEF+KFAAGTEAIA+KLAELSGKGVTTIIGGGDS
Sbjct: 301 GPDAIKSFGSALDTTKTVIWNGPMGVFEFDKFAAGTEAIAKKLAELSGKGVTTIIGGGDS 360

Query: 363 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 404
           VAAVEKVGLA+KMSHISTGGGASLELLEGKPLPGVLALDDA
Sbjct: 361 VAAVEKVGLAEKMSHISTGGGASLELLEGKPLPGVLALDDA 401

BLAST of MS006431 vs. ExPASy Swiss-Prot
Match: Q9SAJ4 (Phosphoglycerate kinase 3, cytosolic OS=Arabidopsis thaliana OX=3702 GN=PGK3 PE=1 SV=1)

HSP 1 Score: 708.0 bits (1826), Expect = 6.2e-203
Identity = 362/401 (90.27%), Postives = 387/401 (96.51%), Query Frame = 0

Query: 3   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 62
           MATKRSVGTLKEADLKGK VFVRVDLNVPLDDN NITDDTRIRAAVPTIKYL+G+G+RV+
Sbjct: 1   MATKRSVGTLKEADLKGKSVFVRVDLNVPLDDNSNITDDTRIRAAVPTIKYLMGNGSRVV 60

Query: 63  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 122
           L SHLGRPKGVTPKYSLKPLVPRLSELLGV+V MANDSIGEEV+KLVA LPEGGVLLLEN
Sbjct: 61  LCSHLGRPKGVTPKYSLKPLVPRLSELLGVEVVMANDSIGEEVQKLVAGLPEGGVLLLEN 120

Query: 123 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKEL 182
           VRFY EEEKNDPEFAKKLA+LAD+YVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKEL
Sbjct: 121 VRFYAEEEKNDPEFAKKLAALADVYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKEL 180

Query: 183 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 242
           DYLVGAVANPK+PFAAIVGGSKVS+KIGVIESLL  V++L+LGGGMIFTFYKAQG SVGS
Sbjct: 181 DYLVGAVANPKKPFAAIVGGSKVSTKIGVIESLLNTVDILLLGGGMIFTFYKAQGLSVGS 240

Query: 243 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 302
           SLVEEDKL LA SL+EKAK+KGVSLLLPTDVVIADKFA DANSK V A++IPDGWMGLDI
Sbjct: 241 SLVEEDKLDLAKSLMEKAKAKGVSLLLPTDVVIADKFAPDANSKIVPATAIPDGWMGLDI 300

Query: 303 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 362
           GPDSIK+FSEALD+T+TIIWNGPMGVFEF+KFAAGTEA+A++LAELSGKGVTTIIGGGDS
Sbjct: 301 GPDSIKTFSEALDTTKTIIWNGPMGVFEFDKFAAGTEAVAKQLAELSGKGVTTIIGGGDS 360

Query: 363 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 404
           VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALD+A
Sbjct: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDEA 401

BLAST of MS006431 vs. ExPASy Swiss-Prot
Match: P12783 (Phosphoglycerate kinase, cytosolic OS=Triticum aestivum OX=4565 PE=2 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 8.1e-195
Identity = 347/401 (86.53%), Postives = 377/401 (94.01%), Query Frame = 0

Query: 3   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 62
           MATKRSVGTL EADLKGK+VFVR DLNVPLDD   ITDDTRIRA++PTIKYLL  GA+VI
Sbjct: 1   MATKRSVGTLGEADLKGKKVFVRADLNVPLDDAQKITDDTRIRASIPTIKYLLEKGAKVI 60

Query: 63  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 122
           L+SHLGRPKGVTPK+SLKPLV RLSELLG++V MA D IGEEVEKL A LP+GGVLLLEN
Sbjct: 61  LASHLGRPKGVTPKFSLKPLVARLSELLGLEVVMAPDCIGEEVEKLAAALPDGGVLLLEN 120

Query: 123 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKEL 182
           VRFYKEEEKNDPEFAKKLAS+ADLYVNDAFGTAHRAHASTEGV KFL+PSVAGFLMQKEL
Sbjct: 121 VRFYKEEEKNDPEFAKKLASVADLYVNDAFGTAHRAHASTEGVTKFLRPSVAGFLMQKEL 180

Query: 183 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 242
           DYLVGAVANPK+PFAAIVGGSKVSSKIGVIESLL KV++L+LGGGMIFTFYKAQG +VG 
Sbjct: 181 DYLVGAVANPKKPFAAIVGGSKVSSKIGVIESLLAKVDILILGGGMIFTFYKAQGLAVGK 240

Query: 243 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 302
           SLVEEDKL LATSLIE AKSKGV LLLPTDVV+ADKFAADA SK V A++IPDGWMGLD+
Sbjct: 241 SLVEEDKLELATSLIETAKSKGVKLLLPTDVVVADKFAADAESKIVPATAIPDGWMGLDV 300

Query: 303 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 362
           GPDSIK+F+EALD+T+T+IWNGPMGVFEFEKFAAGT+AIA++LAEL+GKGVTTIIGGGDS
Sbjct: 301 GPDSIKTFAEALDTTKTVIWNGPMGVFEFEKFAAGTDAIAKQLAELTGKGVTTIIGGGDS 360

Query: 363 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 404
           VAAVEK GLADKMSHISTGGGASLELLEGKPLPGVLALD+A
Sbjct: 361 VAAVEKAGLADKMSHISTGGGASLELLEGKPLPGVLALDEA 401

BLAST of MS006431 vs. ExPASy Swiss-Prot
Match: P29409 (Phosphoglycerate kinase, chloroplastic (Fragment) OS=Spinacia oleracea OX=3562 PE=2 SV=2)

HSP 1 Score: 674.1 bits (1738), Expect = 9.9e-193
Identity = 345/403 (85.61%), Postives = 374/403 (92.80%), Query Frame = 0

Query: 1   IVMATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGAR 60
           +    K+SVG L  ADLKGK+VFVR DLNVPLDD+ NITDDTRIRAA+PTIK+L+ +GA+
Sbjct: 26  VASMAKKSVGDLTSADLKGKKVFVRADLNVPLDDSQNITDDTRIRAAIPTIKHLINNGAK 85

Query: 61  VILSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLL 120
           VILSSHLGRPKGVTPK+SL PLVPRLSELLG+QV  A+D IG +VEKLVAELPEGGVLLL
Sbjct: 86  VILSSHLGRPKGVTPKFSLAPLVPRLSELLGLQVVKADDCIGPDVEKLVAELPEGGVLLL 145

Query: 121 ENVRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQK 180
           ENVRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGV KFLKPSVAGFL+QK
Sbjct: 146 ENVRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQK 205

Query: 181 ELDYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSV 240
           ELDYLVGAV+NPKRPFAAIVGGSKVSSKIGVIESLLEK ++L+LGGGMIFTFYKAQG SV
Sbjct: 206 ELDYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGMSV 265

Query: 241 GSSLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGL 300
           GSSLVEEDKL LATSL+ KAK KGVSLLLPTDVVIADKFAADA+SK V AS IPDGWMGL
Sbjct: 266 GSSLVEEDKLDLATSLLAKAKEKGVSLLLPTDVVIADKFAADADSKIVPASGIPDGWMGL 325

Query: 301 DIGPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGG 360
           DIGPDSIK+FSEALD+TQT+IWNGPMGVFEFEKFAAGTEAIA+KL E+S KG TTIIGGG
Sbjct: 326 DIGPDSIKTFSEALDTTQTVIWNGPMGVFEFEKFAAGTEAIAKKLEEISKKGATTIIGGG 385

Query: 361 DSVAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 404
           DSVAAVEKVG+A+ MSHISTGGGASLELLEGK LPGVLAL++A
Sbjct: 386 DSVAAVEKVGVAEAMSHISTGGGASLELLEGKQLPGVLALNEA 428

BLAST of MS006431 vs. ExPASy Swiss-Prot
Match: Q42961 (Phosphoglycerate kinase, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 672.2 bits (1733), Expect = 3.8e-192
Identity = 342/403 (84.86%), Postives = 374/403 (92.80%), Query Frame = 0

Query: 1   IVMATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGAR 60
           +    K+SVG L  A+LKGK+VFVR DLNVPLDDN NITDDTRIRAAVPTIK+L+ +GA+
Sbjct: 73  VASMAKKSVGDLTAAELKGKKVFVRADLNVPLDDNQNITDDTRIRAAVPTIKHLMANGAK 132

Query: 61  VILSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLL 120
           VILSSHLGRPKGVTPKYSL PLVPRLSELLG+QV    D IG EVEKLVA LPEGGVLLL
Sbjct: 133 VILSSHLGRPKGVTPKYSLAPLVPRLSELLGIQVVKVEDCIGPEVEKLVASLPEGGVLLL 192

Query: 121 ENVRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQK 180
           ENVRFYKEEEKN+PEFAKKLASLADLYVNDAFGTAHRAHASTEGV KFLKPSVAGFL+QK
Sbjct: 193 ENVRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQK 252

Query: 181 ELDYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSV 240
           ELDYLVGAV+NPKRPFAAIVGGSKVSSKIGVIESLLEK ++L+LGGGMIFTFYKAQG SV
Sbjct: 253 ELDYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSV 312

Query: 241 GSSLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGL 300
           GSSLVEEDKL LATSL+EKAK+KGVSLLLP+DVVIADKFA DANSK V AS+IPDGWMGL
Sbjct: 313 GSSLVEEDKLELATSLLEKAKAKGVSLLLPSDVVIADKFAPDANSKIVPASAIPDGWMGL 372

Query: 301 DIGPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGG 360
           DIGPDS+K+F++ALD+T+T+IWNGPMGVFEF+KFA GTEAIA+KLA+LSGKGVTTIIGGG
Sbjct: 373 DIGPDSVKTFNDALDTTKTVIWNGPMGVFEFDKFAVGTEAIAKKLADLSGKGVTTIIGGG 432

Query: 361 DSVAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 404
           DSVAAVEKVG+A  MSHISTGGGASLELLEGK LPGV+ALD+A
Sbjct: 433 DSVAAVEKVGVASVMSHISTGGGASLELLEGKVLPGVIALDEA 475

BLAST of MS006431 vs. ExPASy TrEMBL
Match: A0A6J1D3U8 (Phosphoglycerate kinase OS=Momordica charantia OX=3673 GN=LOC111016741 PE=3 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 2.2e-219
Identity = 400/401 (99.75%), Postives = 401/401 (100.00%), Query Frame = 0

Query: 3   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 62
           MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI
Sbjct: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 60

Query: 63  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 122
           LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN
Sbjct: 61  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 120

Query: 123 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKEL 182
           VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFL+PSVAGFLMQKEL
Sbjct: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLEPSVAGFLMQKEL 180

Query: 183 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 242
           DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS
Sbjct: 181 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 240

Query: 243 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 302
           SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI
Sbjct: 241 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 300

Query: 303 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 362
           GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS
Sbjct: 301 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360

Query: 363 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 404
           VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA
Sbjct: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 401

BLAST of MS006431 vs. ExPASy TrEMBL
Match: A0A6J1JF79 (Phosphoglycerate kinase OS=Cucurbita maxima OX=3661 GN=LOC111486363 PE=3 SV=1)

HSP 1 Score: 746.9 bits (1927), Expect = 4.4e-212
Identity = 384/401 (95.76%), Postives = 395/401 (98.50%), Query Frame = 0

Query: 3   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 62
           MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTI+YLLGHGARVI
Sbjct: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIQYLLGHGARVI 60

Query: 63  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 122
           LS+HLGRPKGVTPKYSLKPL+PRLSELLG+QV MAND IGEEVEKLVAEL EGGVLLLEN
Sbjct: 61  LSTHLGRPKGVTPKYSLKPLIPRLSELLGLQVIMANDCIGEEVEKLVAELSEGGVLLLEN 120

Query: 123 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKEL 182
           VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAK+LKPSVAGFLMQKEL
Sbjct: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKYLKPSVAGFLMQKEL 180

Query: 183 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 242
           DYLVGAV+NPKRPFAAIVGGSKVSSKIGVIESLLEKVNLL LGGGMIFTFYKAQGHSVGS
Sbjct: 181 DYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLFLGGGMIFTFYKAQGHSVGS 240

Query: 243 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 302
           SLVEEDKL LATS+IEKAK+KGVSLLLPTDVVIADKFAADANSK VAAS+IPDGWMGLDI
Sbjct: 241 SLVEEDKLDLATSIIEKAKAKGVSLLLPTDVVIADKFAADANSKVVAASAIPDGWMGLDI 300

Query: 303 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 362
           GPDSIKSFSEALDSTQT+IWNGPMGVFEF+KFAAGTEAIARKLAELSGKGVTTIIGGGDS
Sbjct: 301 GPDSIKSFSEALDSTQTVIWNGPMGVFEFDKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360

Query: 363 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 404
           VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA
Sbjct: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 401

BLAST of MS006431 vs. ExPASy TrEMBL
Match: A0A6J1EXD4 (Phosphoglycerate kinase OS=Cucurbita moschata OX=3662 GN=LOC111437038 PE=3 SV=1)

HSP 1 Score: 744.2 bits (1920), Expect = 2.9e-211
Identity = 383/401 (95.51%), Postives = 394/401 (98.25%), Query Frame = 0

Query: 3   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 62
           MATKRSVGTLKEADLK KRVFVRVDLNVPLDDNFNITDDTRIRAAVPTI+YLLGHGARVI
Sbjct: 1   MATKRSVGTLKEADLKRKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIQYLLGHGARVI 60

Query: 63  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 122
           LS+HLGRPKGVTPKYSLKPL+PRLSELLG+QV MAND IGEEVEKLVAEL EGGVLLLEN
Sbjct: 61  LSTHLGRPKGVTPKYSLKPLIPRLSELLGLQVIMANDCIGEEVEKLVAELSEGGVLLLEN 120

Query: 123 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKEL 182
           VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAK+LKPSVAGFLMQKEL
Sbjct: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKYLKPSVAGFLMQKEL 180

Query: 183 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 242
           DYLVGAV+NPKRPFAAIVGGSKVSSKIGVIESLLEKVNLL LGGGMIFTFYKAQGHSVGS
Sbjct: 181 DYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLFLGGGMIFTFYKAQGHSVGS 240

Query: 243 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 302
           SLVEEDKL LATSLIEKAK+KGVSLLLPTDVV+ADKFAADANSK VAAS+IPDGWMGLDI
Sbjct: 241 SLVEEDKLDLATSLIEKAKAKGVSLLLPTDVVVADKFAADANSKVVAASAIPDGWMGLDI 300

Query: 303 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 362
           GPDSIKSFSEALDSTQT+IWNGPMGVFEF+KFAAGTEAIARKLAELSGKGVTTIIGGGDS
Sbjct: 301 GPDSIKSFSEALDSTQTVIWNGPMGVFEFDKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360

Query: 363 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 404
           VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA
Sbjct: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 401

BLAST of MS006431 vs. ExPASy TrEMBL
Match: A0A0A0K441 (Phosphoglycerate kinase OS=Cucumis sativus OX=3659 GN=Csa_7G239010 PE=3 SV=1)

HSP 1 Score: 738.0 bits (1904), Expect = 2.1e-209
Identity = 381/401 (95.01%), Postives = 392/401 (97.76%), Query Frame = 0

Query: 3   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 62
           MATK+SVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTI+YLL HGARVI
Sbjct: 1   MATKKSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIQYLLSHGARVI 60

Query: 63  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 122
           LSSHLGRPKGVTPKYSLKPLVPRLSELLG+QV MAND IGEEVEK+VAELPEGGVLLLEN
Sbjct: 61  LSSHLGRPKGVTPKYSLKPLVPRLSELLGLQVVMANDCIGEEVEKMVAELPEGGVLLLEN 120

Query: 123 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKEL 182
           VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAK+LKPSVAGFLMQKEL
Sbjct: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKYLKPSVAGFLMQKEL 180

Query: 183 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 242
           DYLVGAV+NPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQG +VG 
Sbjct: 181 DYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGLAVGK 240

Query: 243 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 302
           SLVEEDKL LATSLIEKAK+KGVSLLLPTDVV+ADKFAADA+SK VAASSIPDGWMGLDI
Sbjct: 241 SLVEEDKLDLATSLIEKAKAKGVSLLLPTDVVVADKFAADADSKVVAASSIPDGWMGLDI 300

Query: 303 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 362
           GPDSIKSF EALDSTQTIIWNGPMGVFEFEKFAAGTEAIA+KLAELSGKGVTTIIGGGDS
Sbjct: 301 GPDSIKSFGEALDSTQTIIWNGPMGVFEFEKFAAGTEAIAKKLAELSGKGVTTIIGGGDS 360

Query: 363 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 404
           VAAVEKVGLADKMSHISTGGGASLELLEGK LPGVLALDDA
Sbjct: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKTLPGVLALDDA 401

BLAST of MS006431 vs. ExPASy TrEMBL
Match: A0A6J1GM56 (Phosphoglycerate kinase OS=Cucurbita moschata OX=3662 GN=LOC111455236 PE=3 SV=1)

HSP 1 Score: 736.5 bits (1900), Expect = 6.0e-209
Identity = 382/401 (95.26%), Postives = 391/401 (97.51%), Query Frame = 0

Query: 3   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 62
           MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLG+GARVI
Sbjct: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGNGARVI 60

Query: 63  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 122
           LSSHLGRPKGVTPKYSLKPLVPRLSELLGV V MAND IGEEVEKLVA LPEGGVLLLEN
Sbjct: 61  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVSVVMANDCIGEEVEKLVAGLPEGGVLLLEN 120

Query: 123 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKEL 182
           VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAK+LKPSVAGFLMQKEL
Sbjct: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKYLKPSVAGFLMQKEL 180

Query: 183 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 242
           DYLVGAV+NPKRPFAAIVGGSKVSSKIGVIESLLEKVNLL LGGGMIFTFYKAQG +VG 
Sbjct: 181 DYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLFLGGGMIFTFYKAQGLAVGK 240

Query: 243 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 302
           SLVEEDKL LATSL+EKAK+KGVSLLLPTDVV+ADKFAADA+SK VAASSIPDGWMGLDI
Sbjct: 241 SLVEEDKLDLATSLLEKAKAKGVSLLLPTDVVVADKFAADADSKVVAASSIPDGWMGLDI 300

Query: 303 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 362
           GPDSIKSFSEALDSTQTIIWNGPMGVFEF+KFAAGTEAIARKLAELSGKGVTTIIGGGDS
Sbjct: 301 GPDSIKSFSEALDSTQTIIWNGPMGVFEFDKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360

Query: 363 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 404
           VAAVEKVGLADKMSHISTGGGASLELLEGK LPGVLALDDA
Sbjct: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKTLPGVLALDDA 401

BLAST of MS006431 vs. TAIR 10
Match: AT1G79550.1 (phosphoglycerate kinase )

HSP 1 Score: 708.0 bits (1826), Expect = 4.4e-204
Identity = 362/401 (90.27%), Postives = 387/401 (96.51%), Query Frame = 0

Query: 3   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 62
           MATKRSVGTLKEADLKGK VFVRVDLNVPLDDN NITDDTRIRAAVPTIKYL+G+G+RV+
Sbjct: 1   MATKRSVGTLKEADLKGKSVFVRVDLNVPLDDNSNITDDTRIRAAVPTIKYLMGNGSRVV 60

Query: 63  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 122
           L SHLGRPKGVTPKYSLKPLVPRLSELLGV+V MANDSIGEEV+KLVA LPEGGVLLLEN
Sbjct: 61  LCSHLGRPKGVTPKYSLKPLVPRLSELLGVEVVMANDSIGEEVQKLVAGLPEGGVLLLEN 120

Query: 123 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKEL 182
           VRFY EEEKNDPEFAKKLA+LAD+YVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKEL
Sbjct: 121 VRFYAEEEKNDPEFAKKLAALADVYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKEL 180

Query: 183 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 242
           DYLVGAVANPK+PFAAIVGGSKVS+KIGVIESLL  V++L+LGGGMIFTFYKAQG SVGS
Sbjct: 181 DYLVGAVANPKKPFAAIVGGSKVSTKIGVIESLLNTVDILLLGGGMIFTFYKAQGLSVGS 240

Query: 243 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 302
           SLVEEDKL LA SL+EKAK+KGVSLLLPTDVVIADKFA DANSK V A++IPDGWMGLDI
Sbjct: 241 SLVEEDKLDLAKSLMEKAKAKGVSLLLPTDVVIADKFAPDANSKIVPATAIPDGWMGLDI 300

Query: 303 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 362
           GPDSIK+FSEALD+T+TIIWNGPMGVFEF+KFAAGTEA+A++LAELSGKGVTTIIGGGDS
Sbjct: 301 GPDSIKTFSEALDTTKTIIWNGPMGVFEFDKFAAGTEAVAKQLAELSGKGVTTIIGGGDS 360

Query: 363 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 404
           VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALD+A
Sbjct: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDEA 401

BLAST of MS006431 vs. TAIR 10
Match: AT1G79550.2 (phosphoglycerate kinase )

HSP 1 Score: 708.0 bits (1826), Expect = 4.4e-204
Identity = 362/401 (90.27%), Postives = 387/401 (96.51%), Query Frame = 0

Query: 3   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 62
           MATKRSVGTLKEADLKGK VFVRVDLNVPLDDN NITDDTRIRAAVPTIKYL+G+G+RV+
Sbjct: 1   MATKRSVGTLKEADLKGKSVFVRVDLNVPLDDNSNITDDTRIRAAVPTIKYLMGNGSRVV 60

Query: 63  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 122
           L SHLGRPKGVTPKYSLKPLVPRLSELLGV+V MANDSIGEEV+KLVA LPEGGVLLLEN
Sbjct: 61  LCSHLGRPKGVTPKYSLKPLVPRLSELLGVEVVMANDSIGEEVQKLVAGLPEGGVLLLEN 120

Query: 123 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKEL 182
           VRFY EEEKNDPEFAKKLA+LAD+YVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKEL
Sbjct: 121 VRFYAEEEKNDPEFAKKLAALADVYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKEL 180

Query: 183 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 242
           DYLVGAVANPK+PFAAIVGGSKVS+KIGVIESLL  V++L+LGGGMIFTFYKAQG SVGS
Sbjct: 181 DYLVGAVANPKKPFAAIVGGSKVSTKIGVIESLLNTVDILLLGGGMIFTFYKAQGLSVGS 240

Query: 243 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 302
           SLVEEDKL LA SL+EKAK+KGVSLLLPTDVVIADKFA DANSK V A++IPDGWMGLDI
Sbjct: 241 SLVEEDKLDLAKSLMEKAKAKGVSLLLPTDVVIADKFAPDANSKIVPATAIPDGWMGLDI 300

Query: 303 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 362
           GPDSIK+FSEALD+T+TIIWNGPMGVFEF+KFAAGTEA+A++LAELSGKGVTTIIGGGDS
Sbjct: 301 GPDSIKTFSEALDTTKTIIWNGPMGVFEFDKFAAGTEAVAKQLAELSGKGVTTIIGGGDS 360

Query: 363 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 404
           VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALD+A
Sbjct: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDEA 401

BLAST of MS006431 vs. TAIR 10
Match: AT3G12780.1 (phosphoglycerate kinase 1 )

HSP 1 Score: 666.4 bits (1718), Expect = 1.5e-191
Identity = 342/403 (84.86%), Postives = 369/403 (91.56%), Query Frame = 0

Query: 1   IVMATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGAR 60
           +V   K+SVG L  ADLKGK+VFVR DLNVPLDDN  ITDDTRIRAA+PTIKYL+ +GA+
Sbjct: 74  VVSMAKKSVGDLTSADLKGKKVFVRADLNVPLDDNQTITDDTRIRAAIPTIKYLIENGAK 133

Query: 61  VILSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLL 120
           VILS+HLGRPKGVTPK+SL PLVPRLSELLG++V  A+D IG EVE LVA LPEGGVLLL
Sbjct: 134 VILSTHLGRPKGVTPKFSLAPLVPRLSELLGIEVTKADDCIGPEVESLVASLPEGGVLLL 193

Query: 121 ENVRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQK 180
           ENVRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGV KFLKPSVAGFL+QK
Sbjct: 194 ENVRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQK 253

Query: 181 ELDYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSV 240
           ELDYLVGAV+NPKRPFAAIVGGSKVSSKIGVIESLLEK ++L+LGGGMIFTFYKAQG SV
Sbjct: 254 ELDYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSV 313

Query: 241 GSSLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGL 300
           GSSLVEEDKL LAT L+ KAK+KGVSLLLPTDVV+ADKFA DANSK V AS I DGWMGL
Sbjct: 314 GSSLVEEDKLELATELLAKAKAKGVSLLLPTDVVVADKFAPDANSKIVPASGIEDGWMGL 373

Query: 301 DIGPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGG 360
           DIGPDSIK+F+EALD+TQT+IWNGPMGVFE EKFAAGTEAIA KLAELS KGVTTIIGGG
Sbjct: 374 DIGPDSIKTFNEALDTTQTVIWNGPMGVFEMEKFAAGTEAIANKLAELSEKGVTTIIGGG 433

Query: 361 DSVAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 404
           DSVAAVEKVG+A  MSHISTGGGASLELLEGK LPGV+ALD+A
Sbjct: 434 DSVAAVEKVGVAGVMSHISTGGGASLELLEGKVLPGVIALDEA 476

BLAST of MS006431 vs. TAIR 10
Match: AT1G56190.1 (Phosphoglycerate kinase family protein )

HSP 1 Score: 664.5 bits (1713), Expect = 5.6e-191
Identity = 337/403 (83.62%), Postives = 372/403 (92.31%), Query Frame = 0

Query: 1   IVMATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGAR 60
           ++   K+SVG L   DLKGK+VFVR DLNVPLDDN NITDDTRIRAA+PTIK+L+ +GA+
Sbjct: 71  VITMAKKSVGDLNSVDLKGKKVFVRADLNVPLDDNQNITDDTRIRAAIPTIKFLIENGAK 130

Query: 61  VILSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLL 120
           VILS+HLGRPKGVTPK+SL PLVPRLSELLG++V  A+D IG EVE LVA LPEGGVLLL
Sbjct: 131 VILSTHLGRPKGVTPKFSLAPLVPRLSELLGIEVVKADDCIGPEVETLVASLPEGGVLLL 190

Query: 121 ENVRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQK 180
           ENVRFYKEEEKN+P+FAKKLASLADLYVNDAFGTAHRAHASTEGV KFLKPSVAGFL+QK
Sbjct: 191 ENVRFYKEEEKNEPDFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQK 250

Query: 181 ELDYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSV 240
           ELDYLVGAV+NPKRPFAAIVGGSKVSSKIGVIESLLEK ++L+LGGGMIFTFYKAQG SV
Sbjct: 251 ELDYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSV 310

Query: 241 GSSLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGL 300
           GSSLVEEDKL LAT+L+ KAK++GVSLLLPTDVVIADKFA DANSK V AS+IPDGWMGL
Sbjct: 311 GSSLVEEDKLELATTLLAKAKARGVSLLLPTDVVIADKFAPDANSKIVPASAIPDGWMGL 370

Query: 301 DIGPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGG 360
           DIGPDS+K+F+EALD+TQT+IWNGPMGVFEFEKFA GTEA+A KLAELS KGVTTIIGGG
Sbjct: 371 DIGPDSVKTFNEALDTTQTVIWNGPMGVFEFEKFAKGTEAVANKLAELSKKGVTTIIGGG 430

Query: 361 DSVAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 404
           DSVAAVEKVG+A  MSHISTGGGASLELLEGK LPGV+ALD+A
Sbjct: 431 DSVAAVEKVGVAGVMSHISTGGGASLELLEGKVLPGVVALDEA 473

BLAST of MS006431 vs. TAIR 10
Match: AT1G56190.2 (Phosphoglycerate kinase family protein )

HSP 1 Score: 662.9 bits (1709), Expect = 1.6e-190
Identity = 337/398 (84.67%), Postives = 370/398 (92.96%), Query Frame = 0

Query: 6   KRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVILSS 65
           K+SVG L   DLKGK+VFVR DLNVPLDDN NITDDTRIRAA+PTIK+L+ +GA+VILS+
Sbjct: 3   KKSVGDLNSVDLKGKKVFVRADLNVPLDDNQNITDDTRIRAAIPTIKFLIENGAKVILST 62

Query: 66  HLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLENVRF 125
           HLGRPKGVTPK+SL PLVPRLSELLG++V  A+D IG EVE LVA LPEGGVLLLENVRF
Sbjct: 63  HLGRPKGVTPKFSLAPLVPRLSELLGIEVVKADDCIGPEVETLVASLPEGGVLLLENVRF 122

Query: 126 YKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKELDYL 185
           YKEEEKN+P+FAKKLASLADLYVNDAFGTAHRAHASTEGV KFLKPSVAGFL+QKELDYL
Sbjct: 123 YKEEEKNEPDFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYL 182

Query: 186 VGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGSSLV 245
           VGAV+NPKRPFAAIVGGSKVSSKIGVIESLLEK ++L+LGGGMIFTFYKAQG SVGSSLV
Sbjct: 183 VGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLV 242

Query: 246 EEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDIGPD 305
           EEDKL LAT+L+ KAK++GVSLLLPTDVVIADKFA DANSK V AS+IPDGWMGLDIGPD
Sbjct: 243 EEDKLELATTLLAKAKARGVSLLLPTDVVIADKFAPDANSKIVPASAIPDGWMGLDIGPD 302

Query: 306 SIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDSVAA 365
           S+K+F+EALD+TQT+IWNGPMGVFEFEKFA GTEA+A KLAELS KGVTTIIGGGDSVAA
Sbjct: 303 SVKTFNEALDTTQTVIWNGPMGVFEFEKFAKGTEAVANKLAELSKKGVTTIIGGGDSVAA 362

Query: 366 VEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 404
           VEKVG+A  MSHISTGGGASLELLEGK LPGV+ALD+A
Sbjct: 363 VEKVGVAGVMSHISTGGGASLELLEGKVLPGVVALDEA 400

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022147931.14.5e-21999.75phosphoglycerate kinase, cytosolic [Momordica charantia][more]
XP_023529234.15.4e-21296.01phosphoglycerate kinase, cytosolic [Cucurbita pepo subsp. pepo] >XP_023529235.1 ... [more]
XP_022989221.19.1e-21295.76phosphoglycerate kinase, cytosolic [Cucurbita maxima] >XP_022989222.1 phosphogly... [more]
KAG7023014.11.2e-21195.76hypothetical protein SDJN02_16750 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6589315.13.5e-21195.51hypothetical protein SDJN03_17880, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Q429621.7e-20590.52Phosphoglycerate kinase, cytosolic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q9SAJ46.2e-20390.27Phosphoglycerate kinase 3, cytosolic OS=Arabidopsis thaliana OX=3702 GN=PGK3 PE=... [more]
P127838.1e-19586.53Phosphoglycerate kinase, cytosolic OS=Triticum aestivum OX=4565 PE=2 SV=1[more]
P294099.9e-19385.61Phosphoglycerate kinase, chloroplastic (Fragment) OS=Spinacia oleracea OX=3562 P... [more]
Q429613.8e-19284.86Phosphoglycerate kinase, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1D3U82.2e-21999.75Phosphoglycerate kinase OS=Momordica charantia OX=3673 GN=LOC111016741 PE=3 SV=1[more]
A0A6J1JF794.4e-21295.76Phosphoglycerate kinase OS=Cucurbita maxima OX=3661 GN=LOC111486363 PE=3 SV=1[more]
A0A6J1EXD42.9e-21195.51Phosphoglycerate kinase OS=Cucurbita moschata OX=3662 GN=LOC111437038 PE=3 SV=1[more]
A0A0A0K4412.1e-20995.01Phosphoglycerate kinase OS=Cucumis sativus OX=3659 GN=Csa_7G239010 PE=3 SV=1[more]
A0A6J1GM566.0e-20995.26Phosphoglycerate kinase OS=Cucurbita moschata OX=3662 GN=LOC111455236 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G79550.14.4e-20490.27phosphoglycerate kinase [more]
AT1G79550.24.4e-20490.27phosphoglycerate kinase [more]
AT3G12780.11.5e-19184.86phosphoglycerate kinase 1 [more]
AT1G56190.15.6e-19183.62Phosphoglycerate kinase family protein [more]
AT1G56190.21.6e-19084.67Phosphoglycerate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001576Phosphoglycerate kinasePRINTSPR00477PHGLYCKINASEcoord: 38..60
score: 55.95
coord: 319..344
score: 59.85
coord: 16..32
score: 69.62
coord: 143..165
score: 70.95
coord: 174..196
score: 55.09
coord: 197..216
score: 60.33
coord: 355..366
score: 62.91
coord: 115..130
score: 70.19
coord: 378..395
score: 84.62
IPR001576Phosphoglycerate kinasePFAMPF00162PGKcoord: 13..392
e-value: 8.6E-166
score: 551.6
IPR001576Phosphoglycerate kinasePIRSFPIRSF000724Pgkcoord: 4..403
e-value: 1.6E-156
score: 519.0
IPR001576Phosphoglycerate kinasePANTHERPTHR11406PHOSPHOGLYCERATE KINASEcoord: 3..403
IPR001576Phosphoglycerate kinaseHAMAPMF_00145Phosphoglyc_kinasecoord: 11..402
score: 42.67218
IPR001576Phosphoglycerate kinaseCDDcd00318Phosphoglycerate_kinasecoord: 12..402
e-value: 0.0
score: 621.581
IPR015824Phosphoglycerate kinase, N-terminalGENE3D3.40.50.1260coord: 189..402
e-value: 5.7E-91
score: 305.7
IPR015824Phosphoglycerate kinase, N-terminalGENE3D3.40.50.1260coord: 10..186
e-value: 1.2E-79
score: 268.2
NoneNo IPR availablePIRSRPIRSR000724-2PIRSR000724-2coord: 11..243
e-value: 1.3E-58
score: 196.3
NoneNo IPR availablePANTHERPTHR11406:SF25PHOSPHOGLYCERATE KINASE, CYTOSOLICcoord: 3..403
IPR015911Phosphoglycerate kinase, conserved sitePROSITEPS00111PGLYCERATE_KINASEcoord: 21..31
IPR036043Phosphoglycerate kinase superfamilySUPERFAMILY53748Phosphoglycerate kinasecoord: 10..402

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS006431.1MS006431.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
molecular_function GO:0004618 phosphoglycerate kinase activity