MS006411 (gene) Bitter gourd (TR) v1

Overview


Name	MS006411
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RING-type domain-containing protein
Location	scaffold327: 427450 .. 428716 (+)
RNA-Seq Expression	MS006411
Synteny	MS006411

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGTTGAAGCTCGCCATCTCAATCTCTTCCATCCTCAACTAATAGGAGGAAACAGGTATGCATATATGTTCATCTTCAGTCTTTCCCGTTTTCTGTTTTTCTGGAAGAACGTCTTTATATTCGTTGTGAGTTTGGTTCTGACAATCTAATGGGCGGGGGTTGTGGGTTTACAGAGAATTCGTGAATCCCATTGAAGGAATGGGAGATGCAACGTTGAGTATGTACAACGCTCATATGGGATACGGAGTGCCATTATCGGGAACTACCACCGCCGAGACTCTCCTTCCGGCTTACAATTCTGTAATCGTGGATTCGGTTTCCCCCAAGACGGCCTCCGTCGCAATGAAGTCCGATAGCGGCCTTACTTATCACCTTCCCCTGCCAAGAAAGCGCTCTAGAGAATGTATGAATCCGTACGTCTCCTACCCCTGTCCCCCAACTTCCAAGTCTTCTTGCGGATCTTTCTCCTTTCTTGGAGAAGACATCTCTCTCCAGATCCACCACCAGCAACTCGACATCGACCGCCTCATTTCCCAACACGTAAGCAATCCAAATCAATCGTCTCTGTTTTTGTTCAATTGTGATTATTCAAGTCGTTCCTTCCCCGTTTTAGATTGATTTTGTATTGATCCGATTCTGATCGTAATCCAACAGTTGGAGAAGGTGAGGTCGGAAGTAGAGGAAAGAAGGAAGAGGCAAGCGAGGAGGATAATAGAGGCGATCGAAGTGGGCGTGATGAAGAGGCTGAAAGCGAAGGAGGAAGAGATCGAGAAGATGGGGAAGTTGAATTGGGCGCTAGAAGAAAGAGTGAAATCGTTGTGTATAGAGAATCAGTTATGGCGCGACATGGCACAGACGAACGAAGCCGCCGTGAATGCCCTGAGGAGCAATCTGGAAGCAGTGCTGTGCCAAGAGCAGAACAACCACGGGGCGGGAGGAATCGACGATCCGGCGACGGCATTGGTGGACGACGCGCAGTCGAGCTGCGGGAGCAACGTGGGGGAGGAAAGAGAGGTGGAGGAGGGGTGGCGCGTGGTGAAGGCAGTGAAAGTGGAGGATGAGAGTAGGAATAAGAATAACAATGGCGGTCAAGATACGGGCAGCAGAAATTGCAGGCTGTGCAGAAACTGCGGGAAAGAGGAATCGAGTGTGCTGCTACTTCCGTGCAGGCACCTGTGCCTGTGTACTGTTTGTGGGTCTTCTCTCCACACATGCCCCATCTGTAAATCCACCAAAAACGCCAGTGTACATGTTAACATGTCC

mRNA sequence

ATGGCTGTTGAAGCTCGCCATCTCAATCTCTTCCATCCTCAACTAATAGGAGGAAACAGAGAATTCGTGAATCCCATTGAAGGAATGGGAGATGCAACGTTGAGTATGTACAACGCTCATATGGGATACGGAGTGCCATTATCGGGAACTACCACCGCCGAGACTCTCCTTCCGGCTTACAATTCTGTAATCGTGGATTCGGTTTCCCCCAAGACGGCCTCCGTCGCAATGAAGTCCGATAGCGGCCTTACTTATCACCTTCCCCTGCCAAGAAAGCGCTCTAGAGAATGTATGAATCCGTACGTCTCCTACCCCTGTCCCCCAACTTCCAAGTCTTCTTGCGGATCTTTCTCCTTTCTTGGAGAAGACATCTCTCTCCAGATCCACCACCAGCAACTCGACATCGACCGCCTCATTTCCCAACACTTGGAGAAGGTGAGGTCGGAAGTAGAGGAAAGAAGGAAGAGGCAAGCGAGGAGGATAATAGAGGCGATCGAAGTGGGCGTGATGAAGAGGCTGAAAGCGAAGGAGGAAGAGATCGAGAAGATGGGGAAGTTGAATTGGGCGCTAGAAGAAAGAGTGAAATCGTTGTGTATAGAGAATCAGTTATGGCGCGACATGGCACAGACGAACGAAGCCGCCGTGAATGCCCTGAGGAGCAATCTGGAAGCAGTGCTGTGCCAAGAGCAGAACAACCACGGGGCGGGAGGAATCGACGATCCGGCGACGGCATTGGTGGACGACGCGCAGTCGAGCTGCGGGAGCAACGTGGGGGAGGAAAGAGAGGTGGAGGAGGGGTGGCGCGTGGTGAAGGCAGTGAAAGTGGAGGATGAGAGTAGGAATAAGAATAACAATGGCGGTCAAGATACGGGCAGCAGAAATTGCAGGCTGTGCAGAAACTGCGGGAAAGAGGAATCGAGTGTGCTGCTACTTCCGTGCAGGCACCTGTGCCTGTGTACTGTTTGTGGGTCTTCTCTCCACACATGCCCCATCTGTAAATCCACCAAAAACGCCAGTGTACATGTTAACATGTCC

Coding sequence (CDS)

ATGGCTGTTGAAGCTCGCCATCTCAATCTCTTCCATCCTCAACTAATAGGAGGAAACAGAGAATTCGTGAATCCCATTGAAGGAATGGGAGATGCAACGTTGAGTATGTACAACGCTCATATGGGATACGGAGTGCCATTATCGGGAACTACCACCGCCGAGACTCTCCTTCCGGCTTACAATTCTGTAATCGTGGATTCGGTTTCCCCCAAGACGGCCTCCGTCGCAATGAAGTCCGATAGCGGCCTTACTTATCACCTTCCCCTGCCAAGAAAGCGCTCTAGAGAATGTATGAATCCGTACGTCTCCTACCCCTGTCCCCCAACTTCCAAGTCTTCTTGCGGATCTTTCTCCTTTCTTGGAGAAGACATCTCTCTCCAGATCCACCACCAGCAACTCGACATCGACCGCCTCATTTCCCAACACTTGGAGAAGGTGAGGTCGGAAGTAGAGGAAAGAAGGAAGAGGCAAGCGAGGAGGATAATAGAGGCGATCGAAGTGGGCGTGATGAAGAGGCTGAAAGCGAAGGAGGAAGAGATCGAGAAGATGGGGAAGTTGAATTGGGCGCTAGAAGAAAGAGTGAAATCGTTGTGTATAGAGAATCAGTTATGGCGCGACATGGCACAGACGAACGAAGCCGCCGTGAATGCCCTGAGGAGCAATCTGGAAGCAGTGCTGTGCCAAGAGCAGAACAACCACGGGGCGGGAGGAATCGACGATCCGGCGACGGCATTGGTGGACGACGCGCAGTCGAGCTGCGGGAGCAACGTGGGGGAGGAAAGAGAGGTGGAGGAGGGGTGGCGCGTGGTGAAGGCAGTGAAAGTGGAGGATGAGAGTAGGAATAAGAATAACAATGGCGGTCAAGATACGGGCAGCAGAAATTGCAGGCTGTGCAGAAACTGCGGGAAAGAGGAATCGAGTGTGCTGCTACTTCCGTGCAGGCACCTGTGCCTGTGTACTGTTTGTGGGTCTTCTCTCCACACATGCCCCATCTGTAAATCCACCAAAAACGCCAGTGTACATGTTAACATGTCC

Protein sequence

MAVEARHLNLFHPQLIGGNREFVNPIEGMGDATLSMYNAHMGYGVPLSGTTTAETLLPAYNSVIVDSVSPKTASVAMKSDSGLTYHLPLPRKRSRECMNPYVSYPCPPTSKSSCGSFSFLGEDISLQIHHQQLDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGVMKRLKAKEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRSNLEAVLCQEQNNHGAGGIDDPATALVDDAQSSCGSNVGEEREVEEGWRVVKAVKVEDESRNKNNNGGQDTGSRNCRLCRNCGKEESSVLLLPCRHLCLCTVCGSSLHTCPICKSTKNASVHVNMS

Homology

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BLAST of MS006411 vs. NCBI nr
Match: XP_011659033.1 (probable BOI-related E3 ubiquitin-protein ligase 3 [Cucumis sativus] >KAE8646100.1 hypothetical protein Csa_016409 [Cucumis sativus])

HSP 1 Score: 511.9 bits (1317), Expect = 4.2e-141
Identity = 298/360 (82.78%), Postives = 312/360 (86.67%), Query Frame = 0


Query: 1   MAVEARHLNLFHPQLIGGNREFVNPIEGMGDATLSMYNA-HMGY-GVPL-SGTT-TAETL 60
           MAVEARHLNLFHPQLIGGNRE VN IE  GDA  +MYN+  MGY GVPL SGTT TAETL
Sbjct: 1   MAVEARHLNLFHPQLIGGNRELVNLIE--GDA--NMYNSTGMGYGGVPLVSGTTSTAETL 60

Query: 61  LPAYNSVIVD-SVSPKTASV--AMKSDSGLTYH--LPLPRKRSRECM--NPYVSYPCPPT 120
           LPAYNSVIVD SVSPKTA+V  AMKSDSGLTY+  LPLPRKR+RECM  NP+ SYP  PT
Sbjct: 61  LPAYNSVIVDSSVSPKTAAVSAAMKSDSGLTYNYTLPLPRKRARECMNINPFASYPSAPT 120

Query: 121 SKSSCGSFSFLGEDISLQIHHQQLDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGV 180
           SK SCGSFSFLGEDISLQIH QQLDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGV
Sbjct: 121 SK-SCGSFSFLGEDISLQIHQQQLDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGV 180

Query: 181 MKRLKAKEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRSNLEAVLCQ- 240
           MK+LK+KEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALR+NLE VLCQ 
Sbjct: 181 MKKLKSKEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRNNLEQVLCQV 240

Query: 241 -EQNNH-GAGGIDDPATALVDDAQSSCGSNVGEEREVEEGWRVVKAVKVEDESRNKNN-N 300
            E   H G GGI+DP TALVDDAQSSCGSN GE  E   GWRVVKA  V+   RN NN  
Sbjct: 241 NEDRTHVGGGGIEDPTTALVDDAQSSCGSNEGEGEE-RGGWRVVKAKTVK---RNSNNGG 300

Query: 301 GGQDTGSRNCRLCRNCGKEESSVLLLPCRHLCLCTVCGSSLHTCPICKSTKNASVHVNMS 346
           GG+DTGSR  R+CRNCGKEESSVLLLPCRHLCLCTVCGSS+HTCPICKSTKNASVHVNMS
Sbjct: 301 GGEDTGSRKSRMCRNCGKEESSVLLLPCRHLCLCTVCGSSVHTCPICKSTKNASVHVNMS 351

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BLAST of MS006411 vs. NCBI nr
Match: KAA0056527.1 (putative BOI-related E3 ubiquitin-protein ligase 3 [Cucumis melo var. makuwa] >TYK12096.1 putative BOI-related E3 ubiquitin-protein ligase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 507.7 bits (1306), Expect = 8.0e-140
Identity = 297/365 (81.37%), Postives = 312/365 (85.48%), Query Frame = 0


Query: 1   MAVEARHLNLFHPQLIGGNREFVNPIEGMGDATLSMYNA-HMGY-GVPL-SGTT-TAETL 60
           MAVEARHLNLFHPQLIGGNRE VN IE  GDA  +MYN+  MGY GVPL SGTT TAETL
Sbjct: 1   MAVEARHLNLFHPQLIGGNRELVNLIE--GDA--NMYNSTGMGYGGVPLVSGTTSTAETL 60

Query: 61  LPAYNSVIVD-SVSPKTASV--AMKSDSGLTYH--LPLPRKRSRECM--NPYVSYPCPPT 120
           LPAYNS+IVD SVSPKTA+V  AMKSDSGLTY+  LPLPRKR+RECM  NP+ SYP  PT
Sbjct: 61  LPAYNSIIVDSSVSPKTAAVSAAMKSDSGLTYNYTLPLPRKRTRECMNINPFTSYPSAPT 120

Query: 121 SKSSCGSFSFLGEDISLQIHHQQLDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGV 180
           SK SCGSFSFLGEDISLQIH QQ DIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGV
Sbjct: 121 SK-SCGSFSFLGEDISLQIHQQQHDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGV 180

Query: 181 MKRLKAKEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRSNLEAVLCQ- 240
           MK+LK+KEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALR+NLE VLCQ 
Sbjct: 181 MKKLKSKEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRNNLEQVLCQV 240

Query: 241 -EQNNH-GAGGIDDPATALVDDAQSSCGSNVGE-----EREVEEGWRVVKAVKVEDESRN 300
            E   H G GGI+DP TALVDDAQSSCGSN GE     E E   GWRVVKA  V+   RN
Sbjct: 241 NEDRAHVGGGGIEDPTTALVDDAQSSCGSNEGEGEGEGEGEERGGWRVVKAKTVK---RN 300

Query: 301 KNN-NGGQDTGSRNCRLCRNCGKEESSVLLLPCRHLCLCTVCGSSLHTCPICKSTKNASV 346
            NN  GG+DTGSR  R+CRNCGKEESSVLLLPCRHLCLCTVCGSS+HTCPICKSTKNASV
Sbjct: 301 SNNGGGGEDTGSRKSRMCRNCGKEESSVLLLPCRHLCLCTVCGSSVHTCPICKSTKNASV 357

[Close]

BLAST of MS006411 vs. NCBI nr
Match: XP_008447126.1 (PREDICTED: probable BOI-related E3 ubiquitin-protein ligase 3 [Cucumis melo])

HSP 1 Score: 504.6 bits (1298), Expect = 6.8e-139
Identity = 296/366 (80.87%), Postives = 311/366 (84.97%), Query Frame = 0


Query: 1   MAVEARHLNLFHPQLIGGNREFVNPIEGMGDATLSMYNA-HMGY-GVPL-SGTT-TAETL 60
           MAVEARHLNLFHPQLIGGNRE VN IE  GDA  +MYN+  MGY GVPL SGTT TAETL
Sbjct: 1   MAVEARHLNLFHPQLIGGNRELVNLIE--GDA--NMYNSTGMGYGGVPLVSGTTSTAETL 60

Query: 61  LPAYNSVIVD-SVSPKTASV--AMKSDSGLTYH--LPLPRKRSRECM--NPYVSYPCPPT 120
           LPAYNS+IVD SVSPKTA+V  AMKSDSGLTY+  LPLPRKR+RECM  NP+ SYP  PT
Sbjct: 61  LPAYNSIIVDSSVSPKTAAVSAAMKSDSGLTYNYTLPLPRKRTRECMNINPFTSYPSAPT 120

Query: 121 SKSSCGSFSFLGEDISLQIHHQQLDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGV 180
           SK SCGSFSFLGEDISLQIH QQ DIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGV
Sbjct: 121 SK-SCGSFSFLGEDISLQIHQQQHDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGV 180

Query: 181 MKRLKAKEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRSNLEAVLCQ- 240
           MK+LK+KEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALR+NLE VLCQ 
Sbjct: 181 MKKLKSKEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRNNLEQVLCQV 240

Query: 241 -EQNNH-GAGGIDDPATALVDDAQSSCGSN------VGEEREVEEGWRVVKAVKVEDESR 300
            E   H G GGI+DP TALVDDAQSSCGSN       GE  E   GWRVVKA  V+   R
Sbjct: 241 NEDRAHVGGGGIEDPTTALVDDAQSSCGSNQGQGEGEGEGGEERGGWRVVKAKTVK---R 300

Query: 301 NKNN-NGGQDTGSRNCRLCRNCGKEESSVLLLPCRHLCLCTVCGSSLHTCPICKSTKNAS 346
           N NN  GG+DTGSR  R+CRNCGKEESSVLLLPCRHLCLCTVCGSS+HTCPICKSTKNAS
Sbjct: 301 NSNNGGGGEDTGSRKSRMCRNCGKEESSVLLLPCRHLCLCTVCGSSVHTCPICKSTKNAS 358

[Close]

BLAST of MS006411 vs. NCBI nr
Match: XP_022147836.1 (probable BOI-related E3 ubiquitin-protein ligase 3, partial [Momordica charantia])

HSP 1 Score: 498.0 bits (1281), Expect = 6.3e-137
Identity = 253/254 (99.61%), Postives = 253/254 (99.61%), Query Frame = 0


Query: 1   MAVEARHLNLFHPQLIGGNREFVNPIEGMGDATLSMYNAHMGYGVPLSGTTTAETLLPAY 60
           MAVEARHLNLFHPQLIGGNREFVNPIEGMGD TLSMYNAHMGYGVPLSGTTTAETLLPAY
Sbjct: 1   MAVEARHLNLFHPQLIGGNREFVNPIEGMGDETLSMYNAHMGYGVPLSGTTTAETLLPAY 60

Query: 61  NSVIVDSVSPKTASVAMKSDSGLTYHLPLPRKRSRECMNPYVSYPCPPTSKSSCGSFSFL 120
           NSVIVDSVSPKTASVAMKSDSGLTYHLPLPRKRSRECMNPYVSYPCPPTSKSSCGSFSFL
Sbjct: 61  NSVIVDSVSPKTASVAMKSDSGLTYHLPLPRKRSRECMNPYVSYPCPPTSKSSCGSFSFL 120

Query: 121 GEDISLQIHHQQLDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGVMKRLKAKEEEI 180
           GEDISLQIHHQQLDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGVMKRLKAKEEEI
Sbjct: 121 GEDISLQIHHQQLDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGVMKRLKAKEEEI 180

Query: 181 EKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRSNLEAVLCQEQNNHGAGGIDD 240
           EKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRSNLEAVLCQEQNNHGAGGIDD
Sbjct: 181 EKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRSNLEAVLCQEQNNHGAGGIDD 240

Query: 241 PATALVDDAQSSCG 255
           PATALVDDAQSSCG
Sbjct: 241 PATALVDDAQSSCG 254

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BLAST of MS006411 vs. NCBI nr
Match: XP_038888853.1 (probable BOI-related E3 ubiquitin-protein ligase 3 [Benincasa hispida])

HSP 1 Score: 495.4 bits (1274), Expect = 4.1e-136
Identity = 288/361 (79.78%), Postives = 304/361 (84.21%), Query Frame = 0


Query: 1   MAVEARHLNLFHPQLIGGNREFVNPIEGMGDATLSMYNA-HMGY-GVPL-SGTTTAETLL 60
           MAVEARHLNLFHPQLIGGNRE VN IEG      +MYN+  MGY GVPL SGTTTAETLL
Sbjct: 1   MAVEARHLNLFHPQLIGGNRELVNLIEG----DTNMYNSTGMGYGGVPLVSGTTTAETLL 60

Query: 61  PAYNSVIVDS--VSPKTASV--AMKSDSGLTYH--LPLPRKRSREC--MNPYVSYPCPPT 120
           PAYNSVIVDS    PKTA+V  AMKSDSGLTY+  LPLPRKR+REC  MNP+ SYP  P 
Sbjct: 61  PAYNSVIVDSSVSPPKTAAVSAAMKSDSGLTYNYTLPLPRKRARECMNMNPFASYPSAPV 120

Query: 121 SKSSCGSFSFLGEDISLQIHHQQLDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGV 180
           SK SCGSFSFLGEDISLQIH QQLDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEV V
Sbjct: 121 SK-SCGSFSFLGEDISLQIHQQQLDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVSV 180

Query: 181 MKRLKAKEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRSNLEAVLCQ- 240
           MKRLK KEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALR+NLE VLCQ 
Sbjct: 181 MKRLKLKEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRNNLEQVLCQV 240

Query: 241 -EQNNH-GAGGIDDPAT-ALVDDAQSSCGSN-VGEEREVEEGWRVVKAVKVEDESRNKNN 300
            E   H   GGI+D AT ALVDDAQSSCGSN   EE+E EEGWRVVK     +++  + N
Sbjct: 241 NEDRTHVVGGGIEDQATAALVDDAQSSCGSNEKEEEKEEEEGWRVVK-----EKTVKRKN 300

Query: 301 NGGQDTGSRNCRLCRNCGKEESSVLLLPCRHLCLCTVCGSSLHTCPICKSTKNASVHVNM 346
            GG+ TGSR  R+CRNCGKEESSVLLLPCRHLCLCTVCGSS+HTCPICKSTKNASVHVNM
Sbjct: 301 GGGEGTGSRKSRMCRNCGKEESSVLLLPCRHLCLCTVCGSSVHTCPICKSTKNASVHVNM 351

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BLAST of MS006411 vs. ExPASy Swiss-Prot
Match: Q9LDD1 (Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=3702 GN=BRG3 PE=1 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 1.3e-68
Identity = 172/368 (46.74%), Postives = 225/368 (61.14%), Query Frame = 0


Query: 1   MAVEARHLNLFHPQLIGGNREFVNPIEGMGDATLSMYNAHMGYG-VP-LSGTTTAETLL- 60
           MAVEA HLN     L   NRE ++P+E  G     +YN  M YG VP  + T   +T L 
Sbjct: 1   MAVEAHHLN----PLFSSNREMIHPVEASG----VVYNTQMRYGTVPTFNPTVECQTSLF 60

Query: 61  -PAYNSVIVD---------SVSPKTASVAMKSDSGLTYHLPL-PRKRSRE---CMNP--Y 120
            P YN   VD         ++    +S+   SD+ + +  P+  RKRSRE    +NP  Y
Sbjct: 61  NPIYNISPVDRLVHQSMKPTIQSVDSSLTFNSDNNVDFLRPVSSRKRSREESVVLNPSAY 120

Query: 121 VSYPCPPTSKSSCGSFSFLGEDISLQIHHQQLDIDRLISQHLEKVRSEVEERRKRQARRI 180
           +     PT         FLG+D+S  +     DIDRLIS H+E++R E+EE+RK Q RRI
Sbjct: 121 MQIQKNPTD-----PLMFLGQDLSSNVQQHHFDIDRLISNHVERMRMEIEEKRKTQGRRI 180

Query: 181 IEAIEVGVMKRLKAKEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRSN 240
           +EA+E G+MK L+AK++EI  +GKLN  LEE+VKSLC+ENQ+WRD+AQ+NEA VNALRSN
Sbjct: 181 VEAVEQGLMKTLRAKDDEINHIGKLNLFLEEKVKSLCVENQIWRDVAQSNEATVNALRSN 240

Query: 241 LEAVLCQEQNNHGAGGIDDPATALVDDAQSSCGSNVGEEREVEEGWRVVKAVKVEDESRN 300
           L+ VL   + N      ++P T + DDAQS CGSN   + E EE W++            
Sbjct: 241 LQQVLAAVERNRW----EEPPT-VADDAQSCCGSNDEGDSE-EERWKLA----------- 300

Query: 301 KNNNGGQDTGSRNCR----LCRNCGKEESSVLLLPCRHLCLCTVCGSSLHTCPICKSTKN 346
                 QDT  + CR    +CR+CGK E+SVLLLPCRH+CLC+VCGSSL+TCPICKS K 
Sbjct: 301 ---GEAQDT-KKMCRVGMSMCRSCGKGEASVLLLPCRHMCLCSVCGSSLNTCPICKSPKT 334

[Close]

BLAST of MS006411 vs. ExPASy Swiss-Prot
Match: F4IDI6 (Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=BRG2 PE=1 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 1.8e-62
Identity = 173/371 (46.63%), Postives = 217/371 (58.49%), Query Frame = 0


Query: 1   MAVEARHLNLFHPQLIGGNREFV---NPIE----GMGDATLSMYNAHMGYGVPLSGTTTA 60
           MAV+A HL L  PQL   NRE     N +E    G  +   + Y     + VP   +TT 
Sbjct: 1   MAVDAHHLFLSPPQLF-SNRELTMNNNTMEPTSGGFCNNNQTGYGVVSPFSVPNHTSTTT 60

Query: 61  ETLLPAYNSVIVDSVSPKTASVAMKSDSGLTYH-LPLP-RKRSRE----------CMNPY 120
               P  +        P TA       + L     PLP RKRSR+            NP 
Sbjct: 61  TATPPLLHVYGGSDTIPTTAGYYADGATNLDCEFFPLPTRKRSRDSSRSNYHHLLLQNPR 120

Query: 121 VSYPC--PPTSKSSCGSFSFLGEDISLQIH--HQQLDIDRLISQHL---EKVRSEVEERR 180
            S  C    T+ ++   FSFLG+DI +  H   QQ +IDR +S HL   E+V+ E+EE+R
Sbjct: 121 -SSSCVNAATTTTTTTLFSFLGQDIDISSHMNQQQHEIDRFVSLHLYQMERVKYEIEEKR 180

Query: 181 KRQARRIIEAIEVGVMKRLKAKEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAA 240
           KRQAR I+EAIE G++KRL+ KEEE E++GK+N ALEERVKSL IENQ+WRD+AQTNEA 
Sbjct: 181 KRQARTIMEAIEQGLVKRLRVKEEERERIGKVNHALEERVKSLSIENQIWRDLAQTNEAT 240

Query: 241 VNALRSNLEAVLCQEQNNHGAGGIDDPATALVDDAQSSCGSNVGEEREVEEGWRVVKAVK 300
            N LR+NLE VL Q ++     G++       DDA+S CGS+ G   E      V + V 
Sbjct: 241 ANHLRTNLEHVLAQVKDVSRGAGLEKNMNE-EDDAESCCGSSCGGGGEE----TVRRRVG 300

Query: 301 VEDESRNKNNNGGQDTGSRNCRLCRNCGKEESSVLLLPCRHLCLCTVCGSSLHTCPICKS 346
           +E E+++K          R  R+CRNCG+EES VLLLPCRHLCLC VCGSS+HTCPIC S
Sbjct: 301 LEREAQDKAER-------RRRRMCRNCGEEESCVLLLPCRHLCLCGVCGSSVHTCPICTS 357

[Close]

BLAST of MS006411 vs. ExPASy Swiss-Prot
Match: O81851 (E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.3e-47
Identity = 141/353 (39.94%), Postives = 190/353 (53.82%), Query Frame = 0


Query: 1   MAVEARHLNLFHPQLIGGNREFVNPIEGMGDATLSMYNAHMGYGVPLSGTTTAETLLPAY 60
           MAV+A H+N+F  Q I  NR+ V   E M            G  VPL    T E+     
Sbjct: 1   MAVQAHHMNIF-SQFISPNRDCVKFQENMNHGEFEF----TGGEVPL---ITGESF---- 60

Query: 61  NSVIVDSVSPKTASVAM-KSDSGLTYHL---PLPRKRSRECMNPYVSYPCPPTSKS-SCG 120
                 +V P  A     K++SGL+Y+    PL  KR R+      + P    S +    
Sbjct: 61  ------AVEPLAAKANFNKAESGLSYNFTVPPLSTKRQRDFQFSDSNAPVKRRSVAFDSS 120

Query: 121 SFSFLGEDISLQI-HHQQLDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGVMKRLK 180
           S S +  ++  QI + QQ +IDR ++Q  EK+R E+E R++ Q R +  A++  + K+LK
Sbjct: 121 SPSLINVELVSQIQNQQQSEIDRFVAQQTEKLRIEIEARQQTQTRMLASAVQNVIAKKLK 180

Query: 181 AKEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRSNLEAVLCQEQNNHG 240
            K++EI ++  LNW L+ERVKSL +ENQ+WRD+AQTNEA  N LR+NL+ VL Q +    
Sbjct: 181 EKDDEIVRIRNLNWVLQERVKSLYVENQIWRDIAQTNEANANTLRTNLDQVLAQLETFPT 240

Query: 241 AGGIDDPATALVDDAQSSCGSNVGEEREVEEGWRVVKAVKVEDESRNKNNNGGQDTGSRN 300
           A  +      + DDA+SSCGS  G     + G   V AV            GG       
Sbjct: 241 ASAV------VEDDAESSCGSCCG-----DGGGEAVTAV-----------GGG------- 300

Query: 301 CRLCRNCGKEESSVLLLPCRHLCLCTVCGSS--LHTCPICKSTKNASVHVNMS 346
              C+ CG+ E+SVL+LPCRHLCLCTVCG S  L TCP+C    NASVHVNMS
Sbjct: 301 ---CKRCGEREASVLVLPCRHLCLCTVCGGSALLRTCPVCDMVMNASVHVNMS 303

[Close]

BLAST of MS006411 vs. ExPASy Swiss-Prot
Match: Q9FHE4 (BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG1 PE=1 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 3.7e-47
Identity = 138/355 (38.87%), Postives = 184/355 (51.83%), Query Frame = 0


Query: 1   MAVEARHLNLFHPQLIGGNREFVNPIEGM--------GDATLSMYNAHMGYGVPLSGTTT 60
           MAVEARH+NLF  Q I  NRE V     M        G   +++ + ++ Y  P++    
Sbjct: 1   MAVEARHMNLFSSQYI-TNRECVKSQTNMNNGQQIAGGGFPVTIGDRNLQYIDPINSFNK 60

Query: 61  AETLLPAYNSVIVDSVSPKTASVAMKSDSGLTYHLPLPRKRSRECMNPYVSYPCPPTSKS 120
           +E+ L A +    DS     A +A              +KR     +P            
Sbjct: 61  SESELTAISKRQRDSTFDSDALIA-------------SQKRRAIAFSP------------ 120

Query: 121 SCGSFSFLGEDISLQIHHQQLDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGVMKR 180
                S +  ++  QI  Q  +IDR ++Q  E +R E+E R++ Q R +  A++  ++K+
Sbjct: 121 ----ASLIDAELVSQIQQQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKK 180

Query: 181 LKAKEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRSNLEAVLCQEQNN 240
           LKAK+EEI +MGKLNW L+ERVK+L +ENQ+WRD+AQTNEA  N LRSNLE VL Q  + 
Sbjct: 181 LKAKDEEIIRMGKLNWVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQVLAQVDD- 240

Query: 241 HGAGGIDDPATALVDDAQSSCGSNVGEEREVEEGWRVVKAVKVEDESRNKNNNGGQDTGS 300
                   P     DDA+SSCGS  G +         V AV           NGG     
Sbjct: 241 --LDAFRRPLVEEADDAESSCGSCDGGD---------VTAVV----------NGG----- 293

Query: 301 RNCRLCRNCGKEESSVLLLPCRHLCLCTVCGSS--LHTCPICKSTKNASVHVNMS 346
                C+ CG+  +SVL+LPCRHLCLCTVCGSS  L TCP+C     ASVHVNMS
Sbjct: 301 -----CKRCGELTASVLVLPCRHLCLCTVCGSSALLRTCPVCDMVMTASVHVNMS 293

[Close]

BLAST of MS006411 vs. ExPASy Swiss-Prot
Match: Q8W5R5 (Kinesin-like protein KIN-7D, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=KIN7D PE=2 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 3.9e-04
Identity = 23/78 (29.49%), Postives = 38/78 (48.72%), Query Frame = 0


Query: 257  VGEEREVEEGWRVVKAVKVEDESRNKNNNGGQDTGSRNCRLCRNCGKEESSVLLLPCRHL 316
            V +  E  +   +V  +K   +   +     Q  G  N  +C+ C +  ++ +LLPCRH 
Sbjct: 967  VAKTEETPKEEPLVARLKARMQEMKEKEMKSQANGDANSHICKVCFESPTAAILLPCRHF 1026

Query: 317  CLCTVCGSSLHTCPICKS 335
            CLC  C  +   CPIC++
Sbjct: 1027 CLCKSCSLACSECPICRT 1044

[Close]

BLAST of MS006411 vs. ExPASy TrEMBL
Match: A0A5A7UPI9 (Putative BOI-related E3 ubiquitin-protein ligase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G00260 PE=4 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 3.9e-140
Identity = 297/365 (81.37%), Postives = 312/365 (85.48%), Query Frame = 0


Query: 1   MAVEARHLNLFHPQLIGGNREFVNPIEGMGDATLSMYNA-HMGY-GVPL-SGTT-TAETL 60
           MAVEARHLNLFHPQLIGGNRE VN IE  GDA  +MYN+  MGY GVPL SGTT TAETL
Sbjct: 1   MAVEARHLNLFHPQLIGGNRELVNLIE--GDA--NMYNSTGMGYGGVPLVSGTTSTAETL 60

Query: 61  LPAYNSVIVD-SVSPKTASV--AMKSDSGLTYH--LPLPRKRSRECM--NPYVSYPCPPT 120
           LPAYNS+IVD SVSPKTA+V  AMKSDSGLTY+  LPLPRKR+RECM  NP+ SYP  PT
Sbjct: 61  LPAYNSIIVDSSVSPKTAAVSAAMKSDSGLTYNYTLPLPRKRTRECMNINPFTSYPSAPT 120

Query: 121 SKSSCGSFSFLGEDISLQIHHQQLDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGV 180
           SK SCGSFSFLGEDISLQIH QQ DIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGV
Sbjct: 121 SK-SCGSFSFLGEDISLQIHQQQHDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGV 180

Query: 181 MKRLKAKEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRSNLEAVLCQ- 240
           MK+LK+KEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALR+NLE VLCQ 
Sbjct: 181 MKKLKSKEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRNNLEQVLCQV 240

Query: 241 -EQNNH-GAGGIDDPATALVDDAQSSCGSNVGE-----EREVEEGWRVVKAVKVEDESRN 300
            E   H G GGI+DP TALVDDAQSSCGSN GE     E E   GWRVVKA  V+   RN
Sbjct: 241 NEDRAHVGGGGIEDPTTALVDDAQSSCGSNEGEGEGEGEGEERGGWRVVKAKTVK---RN 300

Query: 301 KNN-NGGQDTGSRNCRLCRNCGKEESSVLLLPCRHLCLCTVCGSSLHTCPICKSTKNASV 346
            NN  GG+DTGSR  R+CRNCGKEESSVLLLPCRHLCLCTVCGSS+HTCPICKSTKNASV
Sbjct: 301 SNNGGGGEDTGSRKSRMCRNCGKEESSVLLLPCRHLCLCTVCGSSVHTCPICKSTKNASV 357

[Close]

BLAST of MS006411 vs. ExPASy TrEMBL
Match: A0A1S3BHA8 (probable BOI-related E3 ubiquitin-protein ligase 3 OS=Cucumis melo OX=3656 GN=LOC103489652 PE=4 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 3.3e-139
Identity = 296/366 (80.87%), Postives = 311/366 (84.97%), Query Frame = 0


Query: 1   MAVEARHLNLFHPQLIGGNREFVNPIEGMGDATLSMYNA-HMGY-GVPL-SGTT-TAETL 60
           MAVEARHLNLFHPQLIGGNRE VN IE  GDA  +MYN+  MGY GVPL SGTT TAETL
Sbjct: 1   MAVEARHLNLFHPQLIGGNRELVNLIE--GDA--NMYNSTGMGYGGVPLVSGTTSTAETL 60

Query: 61  LPAYNSVIVD-SVSPKTASV--AMKSDSGLTYH--LPLPRKRSRECM--NPYVSYPCPPT 120
           LPAYNS+IVD SVSPKTA+V  AMKSDSGLTY+  LPLPRKR+RECM  NP+ SYP  PT
Sbjct: 61  LPAYNSIIVDSSVSPKTAAVSAAMKSDSGLTYNYTLPLPRKRTRECMNINPFTSYPSAPT 120

Query: 121 SKSSCGSFSFLGEDISLQIHHQQLDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGV 180
           SK SCGSFSFLGEDISLQIH QQ DIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGV
Sbjct: 121 SK-SCGSFSFLGEDISLQIHQQQHDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGV 180

Query: 181 MKRLKAKEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRSNLEAVLCQ- 240
           MK+LK+KEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALR+NLE VLCQ 
Sbjct: 181 MKKLKSKEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRNNLEQVLCQV 240

Query: 241 -EQNNH-GAGGIDDPATALVDDAQSSCGSN------VGEEREVEEGWRVVKAVKVEDESR 300
            E   H G GGI+DP TALVDDAQSSCGSN       GE  E   GWRVVKA  V+   R
Sbjct: 241 NEDRAHVGGGGIEDPTTALVDDAQSSCGSNQGQGEGEGEGGEERGGWRVVKAKTVK---R 300

Query: 301 NKNN-NGGQDTGSRNCRLCRNCGKEESSVLLLPCRHLCLCTVCGSSLHTCPICKSTKNAS 346
           N NN  GG+DTGSR  R+CRNCGKEESSVLLLPCRHLCLCTVCGSS+HTCPICKSTKNAS
Sbjct: 301 NSNNGGGGEDTGSRKSRMCRNCGKEESSVLLLPCRHLCLCTVCGSSVHTCPICKSTKNAS 358

[Close]

BLAST of MS006411 vs. ExPASy TrEMBL
Match: A0A6J1D3J4 (probable BOI-related E3 ubiquitin-protein ligase 3 OS=Momordica charantia OX=3673 GN=LOC111016679 PE=4 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 3.1e-137
Identity = 253/254 (99.61%), Postives = 253/254 (99.61%), Query Frame = 0


Query: 1   MAVEARHLNLFHPQLIGGNREFVNPIEGMGDATLSMYNAHMGYGVPLSGTTTAETLLPAY 60
           MAVEARHLNLFHPQLIGGNREFVNPIEGMGD TLSMYNAHMGYGVPLSGTTTAETLLPAY
Sbjct: 1   MAVEARHLNLFHPQLIGGNREFVNPIEGMGDETLSMYNAHMGYGVPLSGTTTAETLLPAY 60

Query: 61  NSVIVDSVSPKTASVAMKSDSGLTYHLPLPRKRSRECMNPYVSYPCPPTSKSSCGSFSFL 120
           NSVIVDSVSPKTASVAMKSDSGLTYHLPLPRKRSRECMNPYVSYPCPPTSKSSCGSFSFL
Sbjct: 61  NSVIVDSVSPKTASVAMKSDSGLTYHLPLPRKRSRECMNPYVSYPCPPTSKSSCGSFSFL 120

Query: 121 GEDISLQIHHQQLDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGVMKRLKAKEEEI 180
           GEDISLQIHHQQLDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGVMKRLKAKEEEI
Sbjct: 121 GEDISLQIHHQQLDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGVMKRLKAKEEEI 180

Query: 181 EKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRSNLEAVLCQEQNNHGAGGIDD 240
           EKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRSNLEAVLCQEQNNHGAGGIDD
Sbjct: 181 EKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRSNLEAVLCQEQNNHGAGGIDD 240

Query: 241 PATALVDDAQSSCG 255
           PATALVDDAQSSCG
Sbjct: 241 PATALVDDAQSSCG 254

[Close]

BLAST of MS006411 vs. ExPASy TrEMBL
Match: A0A0A0K3I3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G237310 PE=4 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 1.1e-126
Identity = 277/346 (80.06%), Postives = 291/346 (84.10%), Query Frame = 0


Query: 1   MAVEARHLNLFHPQLIGGNREFVNPIEGMGDATLSMYNA-HMGY-GVPL-SGTT-TAETL 60
           MAVEARHLNLFHPQLIGGNRE VN IE  GDA  +MYN+  MGY GVPL SGTT TAETL
Sbjct: 1   MAVEARHLNLFHPQLIGGNRELVNLIE--GDA--NMYNSTGMGYGGVPLVSGTTSTAETL 60

Query: 61  LPAYNSVIVD-SVSPKTASV--AMKSDSGLTYH--LPLPRKRSRECM--NPYVSYPCPPT 120
           LPAYNSVIVD SVSPKTA+V  AMKSDSGLTY+  LPLPRKR+RECM  NP+ SYP  PT
Sbjct: 61  LPAYNSVIVDSSVSPKTAAVSAAMKSDSGLTYNYTLPLPRKRARECMNINPFASYPSAPT 120

Query: 121 SKSSCGSFSFLGEDISLQIHHQQLDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGV 180
           SK SCGSFSFLGEDISLQIH QQLDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGV
Sbjct: 121 SK-SCGSFSFLGEDISLQIHQQQLDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGV 180

Query: 181 MKRLKAKEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRSNLEAVLCQ- 240
           MK+LK+KEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALR+NLE VLCQ 
Sbjct: 181 MKKLKSKEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRNNLEQVLCQV 240

Query: 241 -EQNNH-GAGGIDDPATALVDDAQSSCGSNVGEEREVEEGWRVVKAVKVEDESRNKNN-N 300
            E   H G GGI+DP TALVDDAQSSCGSN GE  E   GWRVVKA  V+   RN NN  
Sbjct: 241 NEDRTHVGGGGIEDPTTALVDDAQSSCGSNEGEGEE-RGGWRVVKAKTVK---RNSNNGG 300

Query: 301 GGQDTGSRNCRLCRNCGKEESSVLLLPCRHLCLCTV----CGSSLH 328
           GG+DTGSR  R+CRNCGKEESSVLLLPCRHLCLCT      G S+H
Sbjct: 301 GGEDTGSRKSRMCRNCGKEESSVLLLPCRHLCLCTTIIRGIGGSVH 337

[Close]

BLAST of MS006411 vs. ExPASy TrEMBL
Match: A0A6J1GLP9 (probable BOI-related E3 ubiquitin-protein ligase 3 OS=Cucurbita moschata OX=3662 GN=LOC111455453 PE=4 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 5.4e-126
Identity = 269/371 (72.51%), Postives = 298/371 (80.32%), Query Frame = 0


Query: 1   MAVEARHLNLFHPQLIGGNREFVNPIEGMGDATLSMYNAHMGYGVPL-SGTTTAETLLPA 60
           MAVEARHLNLFHPQLIGGNRE +NPIE  GDA L  YN+ MGYGVPL SGTTT ETLLPA
Sbjct: 1   MAVEARHLNLFHPQLIGGNRELMNPIE--GDANL--YNSQMGYGVPLVSGTTTTETLLPA 60

Query: 61  YNSVIVD-SVSPKTA--SVAMKSDSGLTY----HLPLPRKRSRECM--NPYV-SYPCPPT 120
           YNSVIVD SVSPKTA  S A+KSDSGLT+    +L +PRKRSRECM  NP+  SYP PPT
Sbjct: 61  YNSVIVDSSVSPKTAAPSAAIKSDSGLTFNYNSNLSIPRKRSRECMNINPFASSYPSPPT 120

Query: 121 SKSSCGSFSFLGEDISLQIHHQQLDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGV 180
           SK SCGSFSFLGED+SLQI+ QQLDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEV V
Sbjct: 121 SK-SCGSFSFLGEDLSLQIYQQQLDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVSV 180

Query: 181 MKRLKAKEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRSNLEAVLCQE 240
           MK+LKAKEEE+EKM KLN ALEERVK+L IEN++WRDMAQTNEAAVN LR+NLE VL Q 
Sbjct: 181 MKKLKAKEEEVEKMSKLNLALEERVKTLNIENEVWRDMAQTNEAAVNTLRTNLEQVLRQA 240

Query: 241 QNNHGAGGIDDPATALVDDAQSSCGSNVGEEREVEE------GWRVVKAVKVEDESRNKN 300
           + +   GG+     ALV+DAQSSCGSN  EE+E EE       WRVVK+  V+  ++  N
Sbjct: 241 KEDRKNGGV-GKREALVEDAQSSCGSNEEEEKEEEEEEEVEGRWRVVKSKTVKRNNKGSN 300

Query: 301 -NNGGQDTGSRNC---------RLCRNCGKEESSVLLLPCRHLCLCTVCGSSLHTCPICK 345
             NGG++ GS +          R+CRNCGKEESSVLLLPCRHLCLCTVCGSS+HTCPICK
Sbjct: 301 RKNGGEERGSSSSSSSSSKGSNRMCRNCGKEESSVLLLPCRHLCLCTVCGSSVHTCPICK 360

[Close]

BLAST of MS006411 vs. TAIR 10
Match: AT3G12920.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 261.5 bits (667), Expect = 9.3e-70
Identity = 172/368 (46.74%), Postives = 225/368 (61.14%), Query Frame = 0


Query: 1   MAVEARHLNLFHPQLIGGNREFVNPIEGMGDATLSMYNAHMGYG-VP-LSGTTTAETLL- 60
           MAVEA HLN     L   NRE ++P+E  G     +YN  M YG VP  + T   +T L 
Sbjct: 1   MAVEAHHLN----PLFSSNREMIHPVEASG----VVYNTQMRYGTVPTFNPTVECQTSLF 60

Query: 61  -PAYNSVIVD---------SVSPKTASVAMKSDSGLTYHLPL-PRKRSRE---CMNP--Y 120
            P YN   VD         ++    +S+   SD+ + +  P+  RKRSRE    +NP  Y
Sbjct: 61  NPIYNISPVDRLVHQSMKPTIQSVDSSLTFNSDNNVDFLRPVSSRKRSREESVVLNPSAY 120

Query: 121 VSYPCPPTSKSSCGSFSFLGEDISLQIHHQQLDIDRLISQHLEKVRSEVEERRKRQARRI 180
           +     PT         FLG+D+S  +     DIDRLIS H+E++R E+EE+RK Q RRI
Sbjct: 121 MQIQKNPTD-----PLMFLGQDLSSNVQQHHFDIDRLISNHVERMRMEIEEKRKTQGRRI 180

Query: 181 IEAIEVGVMKRLKAKEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRSN 240
           +EA+E G+MK L+AK++EI  +GKLN  LEE+VKSLC+ENQ+WRD+AQ+NEA VNALRSN
Sbjct: 181 VEAVEQGLMKTLRAKDDEINHIGKLNLFLEEKVKSLCVENQIWRDVAQSNEATVNALRSN 240

Query: 241 LEAVLCQEQNNHGAGGIDDPATALVDDAQSSCGSNVGEEREVEEGWRVVKAVKVEDESRN 300
           L+ VL   + N      ++P T + DDAQS CGSN   + E EE W++            
Sbjct: 241 LQQVLAAVERNRW----EEPPT-VADDAQSCCGSNDEGDSE-EERWKLA----------- 300

Query: 301 KNNNGGQDTGSRNCR----LCRNCGKEESSVLLLPCRHLCLCTVCGSSLHTCPICKSTKN 346
                 QDT  + CR    +CR+CGK E+SVLLLPCRH+CLC+VCGSSL+TCPICKS K 
Sbjct: 301 ---GEAQDT-KKMCRVGMSMCRSCGKGEASVLLLPCRHMCLCSVCGSSLNTCPICKSPKT 334

[Close]

BLAST of MS006411 vs. TAIR 10
Match: AT1G79110.2 (zinc ion binding )

HSP 1 Score: 245.7 bits (626), Expect = 5.3e-65
Identity = 172/368 (46.74%), Postives = 217/368 (58.97%), Query Frame = 0


Query: 1   MAVEARHLNLFHPQLIGGNREFV---NPIE----GMGDATLSMYNAHMGYGVPLSGTTTA 60
           MAV+A HL L  PQL   NRE     N +E    G  +   + Y     + VP   +TT 
Sbjct: 1   MAVDAHHLFLSPPQLF-SNRELTMNNNTMEPTSGGFCNNNQTGYGVVSPFSVPNHTSTTT 60

Query: 61  ETLLPAYNSVIVDSVSPKTASVAMKSDSGLTYH-LPLP-RKRSRE----------CMNPY 120
               P  +        P TA       + L     PLP RKRSR+            NP 
Sbjct: 61  TATPPLLHVYGGSDTIPTTAGYYADGATNLDCEFFPLPTRKRSRDSSRSNYHHLLLQNPR 120

Query: 121 VSYPC--PPTSKSSCGSFSFLGEDISLQIH--HQQLDIDRLISQHLEKVRSEVEERRKRQ 180
            S  C    T+ ++   FSFLG+DI +  H   QQ +IDR +S H+E+V+ E+EE+RKRQ
Sbjct: 121 -SSSCVNAATTTTTTTLFSFLGQDIDISSHMNQQQHEIDRFVSLHMERVKYEIEEKRKRQ 180

Query: 181 ARRIIEAIEVGVMKRLKAKEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNA 240
           AR I+EAIE G++KRL+ KEEE E++GK+N ALEERVKSL IENQ+WRD+AQTNEA  N 
Sbjct: 181 ARTIMEAIEQGLVKRLRVKEEERERIGKVNHALEERVKSLSIENQIWRDLAQTNEATANH 240

Query: 241 LRSNLEAVLCQEQNNHGAGGIDDPATALVDDAQSSCGSNVGEEREVEEGWRVVKAVKVED 300
           LR+NLE VL Q ++     G++       DDA+S CGS+ G   E      V + V +E 
Sbjct: 241 LRTNLEHVLAQVKDVSRGAGLEKNMNE-EDDAESCCGSSCGGGGEE----TVRRRVGLER 300

Query: 301 ESRNKNNNGGQDTGSRNCRLCRNCGKEESSVLLLPCRHLCLCTVCGSSLHTCPICKSTKN 346
           E+++K          R  R+CRNCG+EES VLLLPCRHLCLC VCGSS+HTCPIC S KN
Sbjct: 301 EAQDKAER-------RRRRMCRNCGEEESCVLLLPCRHLCLCGVCGSSVHTCPICTSPKN 354

[Close]

BLAST of MS006411 vs. TAIR 10
Match: AT1G79110.1 (zinc ion binding )

HSP 1 Score: 241.1 bits (614), Expect = 1.3e-63
Identity = 173/371 (46.63%), Postives = 217/371 (58.49%), Query Frame = 0


Query: 1   MAVEARHLNLFHPQLIGGNREFV---NPIE----GMGDATLSMYNAHMGYGVPLSGTTTA 60
           MAV+A HL L  PQL   NRE     N +E    G  +   + Y     + VP   +TT 
Sbjct: 1   MAVDAHHLFLSPPQLF-SNRELTMNNNTMEPTSGGFCNNNQTGYGVVSPFSVPNHTSTTT 60

Query: 61  ETLLPAYNSVIVDSVSPKTASVAMKSDSGLTYH-LPLP-RKRSRE----------CMNPY 120
               P  +        P TA       + L     PLP RKRSR+            NP 
Sbjct: 61  TATPPLLHVYGGSDTIPTTAGYYADGATNLDCEFFPLPTRKRSRDSSRSNYHHLLLQNPR 120

Query: 121 VSYPC--PPTSKSSCGSFSFLGEDISLQIH--HQQLDIDRLISQHL---EKVRSEVEERR 180
            S  C    T+ ++   FSFLG+DI +  H   QQ +IDR +S HL   E+V+ E+EE+R
Sbjct: 121 -SSSCVNAATTTTTTTLFSFLGQDIDISSHMNQQQHEIDRFVSLHLYQMERVKYEIEEKR 180

Query: 181 KRQARRIIEAIEVGVMKRLKAKEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAA 240
           KRQAR I+EAIE G++KRL+ KEEE E++GK+N ALEERVKSL IENQ+WRD+AQTNEA 
Sbjct: 181 KRQARTIMEAIEQGLVKRLRVKEEERERIGKVNHALEERVKSLSIENQIWRDLAQTNEAT 240

Query: 241 VNALRSNLEAVLCQEQNNHGAGGIDDPATALVDDAQSSCGSNVGEEREVEEGWRVVKAVK 300
            N LR+NLE VL Q ++     G++       DDA+S CGS+ G   E      V + V 
Sbjct: 241 ANHLRTNLEHVLAQVKDVSRGAGLEKNMNE-EDDAESCCGSSCGGGGEE----TVRRRVG 300

Query: 301 VEDESRNKNNNGGQDTGSRNCRLCRNCGKEESSVLLLPCRHLCLCTVCGSSLHTCPICKS 346
           +E E+++K          R  R+CRNCG+EES VLLLPCRHLCLC VCGSS+HTCPIC S
Sbjct: 301 LEREAQDKAER-------RRRRMCRNCGEEESCVLLLPCRHLCLCGVCGSSVHTCPICTS 357

[Close]

BLAST of MS006411 vs. TAIR 10
Match: AT4G19700.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 191.8 bits (486), Expect = 9.0e-49
Identity = 141/353 (39.94%), Postives = 190/353 (53.82%), Query Frame = 0


Query: 1   MAVEARHLNLFHPQLIGGNREFVNPIEGMGDATLSMYNAHMGYGVPLSGTTTAETLLPAY 60
           MAV+A H+N+F  Q I  NR+ V   E M            G  VPL    T E+     
Sbjct: 1   MAVQAHHMNIF-SQFISPNRDCVKFQENMNHGEFEF----TGGEVPL---ITGESF---- 60

Query: 61  NSVIVDSVSPKTASVAM-KSDSGLTYHL---PLPRKRSRECMNPYVSYPCPPTSKS-SCG 120
                 +V P  A     K++SGL+Y+    PL  KR R+      + P    S +    
Sbjct: 61  ------AVEPLAAKANFNKAESGLSYNFTVPPLSTKRQRDFQFSDSNAPVKRRSVAFDSS 120

Query: 121 SFSFLGEDISLQI-HHQQLDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGVMKRLK 180
           S S +  ++  QI + QQ +IDR ++Q  EK+R E+E R++ Q R +  A++  + K+LK
Sbjct: 121 SPSLINVELVSQIQNQQQSEIDRFVAQQTEKLRIEIEARQQTQTRMLASAVQNVIAKKLK 180

Query: 181 AKEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRSNLEAVLCQEQNNHG 240
            K++EI ++  LNW L+ERVKSL +ENQ+WRD+AQTNEA  N LR+NL+ VL Q +    
Sbjct: 181 EKDDEIVRIRNLNWVLQERVKSLYVENQIWRDIAQTNEANANTLRTNLDQVLAQLETFPT 240

Query: 241 AGGIDDPATALVDDAQSSCGSNVGEEREVEEGWRVVKAVKVEDESRNKNNNGGQDTGSRN 300
           A  +      + DDA+SSCGS  G     + G   V AV            GG       
Sbjct: 241 ASAV------VEDDAESSCGSCCG-----DGGGEAVTAV-----------GGG------- 300

Query: 301 CRLCRNCGKEESSVLLLPCRHLCLCTVCGSS--LHTCPICKSTKNASVHVNMS 346
              C+ CG+ E+SVL+LPCRHLCLCTVCG S  L TCP+C    NASVHVNMS
Sbjct: 301 ---CKRCGEREASVLVLPCRHLCLCTVCGGSALLRTCPVCDMVMNASVHVNMS 303

[Close]

BLAST of MS006411 vs. TAIR 10
Match: AT5G45100.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 190.3 bits (482), Expect = 2.6e-48
Identity = 138/355 (38.87%), Postives = 184/355 (51.83%), Query Frame = 0


Query: 1   MAVEARHLNLFHPQLIGGNREFVNPIEGM--------GDATLSMYNAHMGYGVPLSGTTT 60
           MAVEARH+NLF  Q I  NRE V     M        G   +++ + ++ Y  P++    
Sbjct: 1   MAVEARHMNLFSSQYI-TNRECVKSQTNMNNGQQIAGGGFPVTIGDRNLQYIDPINSFNK 60

Query: 61  AETLLPAYNSVIVDSVSPKTASVAMKSDSGLTYHLPLPRKRSRECMNPYVSYPCPPTSKS 120
           +E+ L A +    DS     A +A              +KR     +P            
Sbjct: 61  SESELTAISKRQRDSTFDSDALIA-------------SQKRRAIAFSP------------ 120

Query: 121 SCGSFSFLGEDISLQIHHQQLDIDRLISQHLEKVRSEVEERRKRQARRIIEAIEVGVMKR 180
                S +  ++  QI  Q  +IDR ++Q  E +R E+E R++ Q R +  A++  ++K+
Sbjct: 121 ----ASLIDAELVSQIQQQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKK 180

Query: 181 LKAKEEEIEKMGKLNWALEERVKSLCIENQLWRDMAQTNEAAVNALRSNLEAVLCQEQNN 240
           LKAK+EEI +MGKLNW L+ERVK+L +ENQ+WRD+AQTNEA  N LRSNLE VL Q  + 
Sbjct: 181 LKAKDEEIIRMGKLNWVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQVLAQVDD- 240

Query: 241 HGAGGIDDPATALVDDAQSSCGSNVGEEREVEEGWRVVKAVKVEDESRNKNNNGGQDTGS 300
                   P     DDA+SSCGS  G +         V AV           NGG     
Sbjct: 241 --LDAFRRPLVEEADDAESSCGSCDGGD---------VTAVV----------NGG----- 293

Query: 301 RNCRLCRNCGKEESSVLLLPCRHLCLCTVCGSS--LHTCPICKSTKNASVHVNMS 346
                C+ CG+  +SVL+LPCRHLCLCTVCGSS  L TCP+C     ASVHVNMS
Sbjct: 301 -----CKRCGELTASVLVLPCRHLCLCTVCGSSALLRTCPVCDMVMTASVHVNMS 293

The following BLAST results are available for this feature:

BLAST of MS006411 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011659033.1	4.2e-141	82.78	probable BOI-related E3 ubiquitin-protein ligase 3 [Cucumis sativus] >KAE8646100...	[more]
KAA0056527.1	8.0e-140	81.37	putative BOI-related E3 ubiquitin-protein ligase 3 [Cucumis melo var. makuwa] >T...	[more]
XP_008447126.1	6.8e-139	80.87	PREDICTED: probable BOI-related E3 ubiquitin-protein ligase 3 [Cucumis melo]	[more]
XP_022147836.1	6.3e-137	99.61	probable BOI-related E3 ubiquitin-protein ligase 3, partial [Momordica charantia...	[more]
XP_038888853.1	4.1e-136	79.78	probable BOI-related E3 ubiquitin-protein ligase 3 [Benincasa hispida]	[more]

BLAST of MS006411 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LDD1	1.3e-68	46.74	Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=37...	[more]
F4IDI6	1.8e-62	46.63	Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=37...	[more]
O81851	1.3e-47	39.94	E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1	[more]
Q9FHE4	3.7e-47	38.87	BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG...	[more]
Q8W5R5	3.9e-04	29.49	Kinesin-like protein KIN-7D, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=KI...	[more]

BLAST of MS006411 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UPI9	3.9e-140	81.37	Putative BOI-related E3 ubiquitin-protein ligase 3 OS=Cucumis melo var. makuwa O...	[more]
A0A1S3BHA8	3.3e-139	80.87	probable BOI-related E3 ubiquitin-protein ligase 3 OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A6J1D3J4	3.1e-137	99.61	probable BOI-related E3 ubiquitin-protein ligase 3 OS=Momordica charantia OX=367...	[more]
A0A0A0K3I3	1.1e-126	80.06	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G237310 PE=4 SV=1	[more]
A0A6J1GLP9	5.4e-126	72.51	probable BOI-related E3 ubiquitin-protein ligase 3 OS=Cucurbita moschata OX=3662...	[more]

BLAST of MS006411 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12920.1	9.3e-70	46.74	SBP (S-ribonuclease binding protein) family protein	[more]
AT1G79110.2	5.3e-65	46.74	zinc ion binding	[more]
AT1G79110.1	1.3e-63	46.63	zinc ion binding	[more]
AT4G19700.1	9.0e-49	39.94	SBP (S-ribonuclease binding protein) family protein	[more]
AT5G45100.1	2.6e-48	38.87	SBP (S-ribonuclease binding protein) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 173..193
None	No IPR available	PFAM	PF13920	zf-C3HC4_3	coord: 296..334 e-value: 9.5E-8 score: 31.8
None	No IPR available	PANTHER	PTHR42647:SF12	BOI-RELATED E3 UBIQUITIN-PROTEIN LIGASE 2-RELATED	coord: 1..344
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 270..345 e-value: 8.5E-11 score: 43.1
IPR017066	E3 ubiquitin-protein ligase BOI-like	PANTHER	PTHR42647	SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEIN	coord: 1..344
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 295..333 score: 10.556635

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS006411.1	MS006411.1	mRNA