MS006386 (gene) Bitter gourd (TR) v1

Overview


Name	MS006386
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	V-type proton ATPase subunit D
Location	scaffold327: 263851 .. 264633 (+)
RNA-Seq Expression	MS006386
Synteny	MS006386

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCGGCCAAAGCCAGCGTTTGAATGTGGTTCCAACAGTTACGATGCTTGGAGCCATGAAAGCCCGTCTTATTGGGGCAACCAGAGGTCATGCTCTCCTCAAGAAGAAGAGTGATGCTTTGACTGTGCAGTTCCGTCAGATACTCAAGAAGATTGTCTCAGCTAAGGAATCAATGGGGGAAGTTATGAAAACATCTTCATTTTCCCTCACCGAGGCAAAATATGTTGCTGGTGACAACATCAAGCATATCGTCCTTGAGAATGTCCAAAATGCAGCTATTAAGATTCGATCTCGGCAGGAGAACATTGCTGGTGTAAAACTCCCAAAGTTTGAACACTATTCTGATGGTGAGACAAAAAATGATCTCACGGGGTTGGCCAGGGGTGGACAACAGATTCAGTTATGTCGGGGTGCCTATGTAAAGGCAATTGAGGTTCTTGTTGAGCTTGCTTCCCTTCAGACATCATTTTTGACTCTTGATGAGGCAATTAAGACCACAAACCGCAGGGTTAATGCTCTAGAGAATGTTGTGAAGCCAAGGATAGAGAACACTATAAGTTACATCAAGGGGGAATTGGACGAGCTGGAAAGGGAAGATTTCTTTAGACTTAAAAAGATACAGGGTTACAAGAAAAGGGAAATTGAGAGACAACGTGCTAATGCCAAGCTATATGCTGAGGAACAGCTTGCTGAGAAGGTGTCTTTGCAAAAGGGTATGTCCATAAGTTCAGCTCACAATTTGTTGTCTGCAGCTATGGAGAAGGACGAGGATATTATTTTT

mRNA sequence

ATGTCCGGCCAAAGCCAGCGTTTGAATGTGGTTCCAACAGTTACGATGCTTGGAGCCATGAAAGCCCGTCTTATTGGGGCAACCAGAGGTCATGCTCTCCTCAAGAAGAAGAGTGATGCTTTGACTGTGCAGTTCCGTCAGATACTCAAGAAGATTGTCTCAGCTAAGGAATCAATGGGGGAAGTTATGAAAACATCTTCATTTTCCCTCACCGAGGCAAAATATGTTGCTGGTGACAACATCAAGCATATCGTCCTTGAGAATGTCCAAAATGCAGCTATTAAGATTCGATCTCGGCAGGAGAACATTGCTGGTGTAAAACTCCCAAAGTTTGAACACTATTCTGATGGTGAGACAAAAAATGATCTCACGGGGTTGGCCAGGGGTGGACAACAGATTCAGTTATGTCGGGGTGCCTATGTAAAGGCAATTGAGGTTCTTGTTGAGCTTGCTTCCCTTCAGACATCATTTTTGACTCTTGATGAGGCAATTAAGACCACAAACCGCAGGGTTAATGCTCTAGAGAATGTTGTGAAGCCAAGGATAGAGAACACTATAAGTTACATCAAGGGGGAATTGGACGAGCTGGAAAGGGAAGATTTCTTTAGACTTAAAAAGATACAGGGTTACAAGAAAAGGGAAATTGAGAGACAACGTGCTAATGCCAAGCTATATGCTGAGGAACAGCTTGCTGAGAAGGTGTCTTTGCAAAAGGGTATGTCCATAAGTTCAGCTCACAATTTGTTGTCTGCAGCTATGGAGAAGGACGAGGATATTATTTTT

Coding sequence (CDS)

ATGTCCGGCCAAAGCCAGCGTTTGAATGTGGTTCCAACAGTTACGATGCTTGGAGCCATGAAAGCCCGTCTTATTGGGGCAACCAGAGGTCATGCTCTCCTCAAGAAGAAGAGTGATGCTTTGACTGTGCAGTTCCGTCAGATACTCAAGAAGATTGTCTCAGCTAAGGAATCAATGGGGGAAGTTATGAAAACATCTTCATTTTCCCTCACCGAGGCAAAATATGTTGCTGGTGACAACATCAAGCATATCGTCCTTGAGAATGTCCAAAATGCAGCTATTAAGATTCGATCTCGGCAGGAGAACATTGCTGGTGTAAAACTCCCAAAGTTTGAACACTATTCTGATGGTGAGACAAAAAATGATCTCACGGGGTTGGCCAGGGGTGGACAACAGATTCAGTTATGTCGGGGTGCCTATGTAAAGGCAATTGAGGTTCTTGTTGAGCTTGCTTCCCTTCAGACATCATTTTTGACTCTTGATGAGGCAATTAAGACCACAAACCGCAGGGTTAATGCTCTAGAGAATGTTGTGAAGCCAAGGATAGAGAACACTATAAGTTACATCAAGGGGGAATTGGACGAGCTGGAAAGGGAAGATTTCTTTAGACTTAAAAAGATACAGGGTTACAAGAAAAGGGAAATTGAGAGACAACGTGCTAATGCCAAGCTATATGCTGAGGAACAGCTTGCTGAGAAGGTGTCTTTGCAAAAGGGTATGTCCATAAGTTCAGCTCACAATTTGTTGTCTGCAGCTATGGAGAAGGACGAGGATATTATTTTT

Protein sequence

MSGQSQRLNVVPTVTMLGAMKARLIGATRGHALLKKKSDALTVQFRQILKKIVSAKESMGEVMKTSSFSLTEAKYVAGDNIKHIVLENVQNAAIKIRSRQENIAGVKLPKFEHYSDGETKNDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKPRIENTISYIKGELDELEREDFFRLKKIQGYKKREIERQRANAKLYAEEQLAEKVSLQKGMSISSAHNLLSAAMEKDEDIIF

Homology

[Close]

BLAST of MS006386 vs. NCBI nr
Match: XP_022148058.1 (V-type proton ATPase subunit D [Momordica charantia])

HSP 1 Score: 485.0 bits (1247), Expect = 4.2e-133
Identity = 261/261 (100.00%), Postives = 261/261 (100.00%), Query Frame = 0


Query: 1   MSGQSQRLNVVPTVTMLGAMKARLIGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG 60
           MSGQSQRLNVVPTVTMLGAMKARLIGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG
Sbjct: 1   MSGQSQRLNVVPTVTMLGAMKARLIGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG 60

Query: 61  EVMKTSSFSLTEAKYVAGDNIKHIVLENVQNAAIKIRSRQENIAGVKLPKFEHYSDGETK 120
           EVMKTSSFSLTEAKYVAGDNIKHIVLENVQNAAIKIRSRQENIAGVKLPKFEHYSDGETK
Sbjct: 61  EVMKTSSFSLTEAKYVAGDNIKHIVLENVQNAAIKIRSRQENIAGVKLPKFEHYSDGETK 120

Query: 121 NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 180
           NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP
Sbjct: 121 NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 180

Query: 181 RIENTISYIKGELDELEREDFFRLKKIQGYKKREIERQRANAKLYAEEQLAEKVSLQKGM 240
           RIENTISYIKGELDELEREDFFRLKKIQGYKKREIERQRANAKLYAEEQLAEKVSLQKGM
Sbjct: 181 RIENTISYIKGELDELEREDFFRLKKIQGYKKREIERQRANAKLYAEEQLAEKVSLQKGM 240

Query: 241 SISSAHNLLSAAMEKDEDIIF 262
           SISSAHNLLSAAMEKDEDIIF
Sbjct: 241 SISSAHNLLSAAMEKDEDIIF 261

[Close]

BLAST of MS006386 vs. NCBI nr
Match: XP_022948069.1 (V-type proton ATPase subunit D-like [Cucurbita moschata] >XP_023007390.1 V-type proton ATPase subunit D-like [Cucurbita maxima] >XP_023534555.1 V-type proton ATPase subunit D-like [Cucurbita pepo subsp. pepo] >KAG7035231.1 V-type proton ATPase subunit D, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 472.6 bits (1215), Expect = 2.2e-129
Identity = 254/261 (97.32%), Postives = 260/261 (99.62%), Query Frame = 0


Query: 1   MSGQSQRLNVVPTVTMLGAMKARLIGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG 60
           MSGQ+QRLNVVPTVTMLGAMKARLIGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG
Sbjct: 1   MSGQTQRLNVVPTVTMLGAMKARLIGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG 60

Query: 61  EVMKTSSFSLTEAKYVAGDNIKHIVLENVQNAAIKIRSRQENIAGVKLPKFEHYSDGETK 120
           EVMKTSSFSLTEAKYVAGDNIKHIVLENVQNAAIKIRSRQ+NIAGVKLPKFEHYSDGETK
Sbjct: 61  EVMKTSSFSLTEAKYVAGDNIKHIVLENVQNAAIKIRSRQDNIAGVKLPKFEHYSDGETK 120

Query: 121 NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 180
           NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP
Sbjct: 121 NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 180

Query: 181 RIENTISYIKGELDELEREDFFRLKKIQGYKKREIERQRANAKLYAEEQLAEKVSLQKGM 240
           RIENTISYIKGELDELEREDFFRLKKIQGYK+REIERQRANAKLYAEEQLAEKVSLQKG+
Sbjct: 181 RIENTISYIKGELDELEREDFFRLKKIQGYKRREIERQRANAKLYAEEQLAEKVSLQKGV 240

Query: 241 SISSAHNLLSAAMEKDEDIIF 262
           SISSA++LLSAA EKDEDIIF
Sbjct: 241 SISSAYSLLSAAGEKDEDIIF 261

[Close]

BLAST of MS006386 vs. NCBI nr
Match: KGN48252.2 (hypothetical protein Csa_003783 [Cucumis sativus])

HSP 1 Score: 472.2 bits (1214), Expect = 2.8e-129
Identity = 252/261 (96.55%), Postives = 259/261 (99.23%), Query Frame = 0


Query: 1   MSGQSQRLNVVPTVTMLGAMKARLIGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG 60
           MSGQ+QRLNVVPTVTMLGAMKARL+GATRGHALLKKKSDALTVQFRQILKKIVSAKESMG
Sbjct: 617 MSGQTQRLNVVPTVTMLGAMKARLVGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG 676

Query: 61  EVMKTSSFSLTEAKYVAGDNIKHIVLENVQNAAIKIRSRQENIAGVKLPKFEHYSDGETK 120
           +VMKTS+FSLTEAKYVAGDNIKHIVLENVQ AAIKIRSRQENIAGVKLPKFEHYSDGETK
Sbjct: 677 DVMKTSAFSLTEAKYVAGDNIKHIVLENVQTAAIKIRSRQENIAGVKLPKFEHYSDGETK 736

Query: 121 NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 180
           NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP
Sbjct: 737 NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 796

Query: 181 RIENTISYIKGELDELEREDFFRLKKIQGYKKREIERQRANAKLYAEEQLAEKVSLQKGM 240
           RIENTISYIKGELDELEREDFFRLKKIQGYK+REIERQRANAKL+AEEQLAEKVSLQKG+
Sbjct: 797 RIENTISYIKGELDELEREDFFRLKKIQGYKRREIERQRANAKLFAEEQLAEKVSLQKGV 856

Query: 241 SISSAHNLLSAAMEKDEDIIF 262
           SISSAHNLLSAA EKDEDIIF
Sbjct: 857 SISSAHNLLSAAAEKDEDIIF 877

[Close]

BLAST of MS006386 vs. NCBI nr
Match: XP_004140139.1 (V-type proton ATPase subunit D [Cucumis sativus] >XP_031743924.1 V-type proton ATPase subunit D [Cucumis sativus])

HSP 1 Score: 472.2 bits (1214), Expect = 2.8e-129
Identity = 252/261 (96.55%), Postives = 259/261 (99.23%), Query Frame = 0


Query: 1   MSGQSQRLNVVPTVTMLGAMKARLIGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG 60
           MSGQ+QRLNVVPTVTMLGAMKARL+GATRGHALLKKKSDALTVQFRQILKKIVSAKESMG
Sbjct: 1   MSGQTQRLNVVPTVTMLGAMKARLVGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG 60

Query: 61  EVMKTSSFSLTEAKYVAGDNIKHIVLENVQNAAIKIRSRQENIAGVKLPKFEHYSDGETK 120
           +VMKTS+FSLTEAKYVAGDNIKHIVLENVQ AAIKIRSRQENIAGVKLPKFEHYSDGETK
Sbjct: 61  DVMKTSAFSLTEAKYVAGDNIKHIVLENVQTAAIKIRSRQENIAGVKLPKFEHYSDGETK 120

Query: 121 NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 180
           NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP
Sbjct: 121 NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 180

Query: 181 RIENTISYIKGELDELEREDFFRLKKIQGYKKREIERQRANAKLYAEEQLAEKVSLQKGM 240
           RIENTISYIKGELDELEREDFFRLKKIQGYK+REIERQRANAKL+AEEQLAEKVSLQKG+
Sbjct: 181 RIENTISYIKGELDELEREDFFRLKKIQGYKRREIERQRANAKLFAEEQLAEKVSLQKGV 240

Query: 241 SISSAHNLLSAAMEKDEDIIF 262
           SISSAHNLLSAA EKDEDIIF
Sbjct: 241 SISSAHNLLSAAAEKDEDIIF 261

[Close]

BLAST of MS006386 vs. NCBI nr
Match: KAG6589288.1 (Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 470.7 bits (1210), Expect = 8.2e-129
Identity = 250/261 (95.79%), Postives = 259/261 (99.23%), Query Frame = 0


Query: 1   MSGQSQRLNVVPTVTMLGAMKARLIGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG 60
           MSGQ+QRLNVVPTVTMLGAMKARL+GATRGHALLKKKSDALTVQFRQILKKIVSAKESMG
Sbjct: 591 MSGQTQRLNVVPTVTMLGAMKARLVGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG 650

Query: 61  EVMKTSSFSLTEAKYVAGDNIKHIVLENVQNAAIKIRSRQENIAGVKLPKFEHYSDGETK 120
           EVMKTS+FSLTEAKYVAGDNIKHIVLENVQ+AAIKIRSRQENIAGVKLPKFEHYSDGETK
Sbjct: 651 EVMKTSAFSLTEAKYVAGDNIKHIVLENVQSAAIKIRSRQENIAGVKLPKFEHYSDGETK 710

Query: 121 NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 180
           NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP
Sbjct: 711 NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 770

Query: 181 RIENTISYIKGELDELEREDFFRLKKIQGYKKREIERQRANAKLYAEEQLAEKVSLQKGM 240
           RIENTISYIKGELDELEREDFFRLKKIQGYK+REIERQR NAKLYAEEQLAEK+SLQKG+
Sbjct: 771 RIENTISYIKGELDELEREDFFRLKKIQGYKRREIERQRINAKLYAEEQLAEKISLQKGV 830

Query: 241 SISSAHNLLSAAMEKDEDIIF 262
           S++SAHNLLSAA EKDEDIIF
Sbjct: 831 SMTSAHNLLSAAAEKDEDIIF 851

[Close]

BLAST of MS006386 vs. ExPASy Swiss-Prot
Match: Q9XGM1 (V-type proton ATPase subunit D OS=Arabidopsis thaliana OX=3702 GN=VHA-D PE=1 SV=2)

HSP 1 Score: 410.2 bits (1053), Expect = 1.7e-113
Identity = 210/261 (80.46%), Postives = 240/261 (91.95%), Query Frame = 0


Query: 1   MSGQSQRLNVVPTVTMLGAMKARLIGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG 60
           M+GQ+ RLNVVPTVTMLG MKARL+GATRGHALLKKKSDALTVQFR +LKKIV+AKESMG
Sbjct: 1   MAGQNARLNVVPTVTMLGVMKARLVGATRGHALLKKKSDALTVQFRALLKKIVTAKESMG 60

Query: 61  EVMKTSSFSLTEAKYVAGDNIKHIVLENVQNAAIKIRSRQENIAGVKLPKFEHYSDGETK 120
           ++MKTSSF+LTE KYVAGDN+KH+VLENV+ A +K+RSR ENIAGVKLPKF+H+S+GETK
Sbjct: 61  DMMKTSSFALTEVKYVAGDNVKHVVLENVKEATLKVRSRTENIAGVKLPKFDHFSEGETK 120

Query: 121 NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 180
           NDLTGLARGGQQ++ CR AYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP
Sbjct: 121 NDLTGLARGGQQVRACRVAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 180

Query: 181 RIENTISYIKGELDELEREDFFRLKKIQGYKKREIERQRANAKLYAEEQLAEKVSLQKGM 240
           ++ENTISYIKGELDELEREDFFRLKKIQGYK+RE+ERQ ANAK +AEE + E +S+Q+G+
Sbjct: 181 KLENTISYIKGELDELEREDFFRLKKIQGYKRREVERQAANAKEFAEEMVLEDISMQRGI 240

Query: 241 SISSAHNLLSAAMEKDEDIIF 262
           SI++A N L    EKD DIIF
Sbjct: 241 SINAARNFLVGGAEKDSDIIF 261

[Close]

BLAST of MS006386 vs. ExPASy Swiss-Prot
Match: P57746 (V-type proton ATPase subunit D OS=Mus musculus OX=10090 GN=Atp6v1d PE=1 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 4.5e-61
Identity = 141/268 (52.61%), Postives = 183/268 (68.28%), Query Frame = 0


Query: 1   MSGQSQRLNVVPTVTMLGAMKARLIGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG 60
           MSG+  R+ + P+      MKARL GA  G  LLKKKSDALT++FRQILKKI+  K  MG
Sbjct: 1   MSGK-DRIEIFPSRMAQTIMKARLKGAQTGRNLLKKKSDALTLRFRQILKKIIETKMLMG 60

Query: 61  EVMKTSSFSLTEAKYVAGDNIKHIVLENVQNAAIKIRSRQENIAGVKLPKFEHYSDGETK 120
           EVM+ ++FSL EAK+ AGD     V++NV  A +KIR++++N+AGV LP FEHY +G   
Sbjct: 61  EVMREAAFSLAEAKFTAGD-FSTTVIQNVNKAQVKIRAKKDNVAGVTLPVFEHYHEGTDS 120

Query: 121 NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 180
            +LTGLARGG+Q+   +  Y KA+E+LVELASLQTSF+TLDEAIK TNRRVNA+E+V+ P
Sbjct: 121 YELTGLARGGEQLAKLKRNYAKAVELLVELASLQTSFVTLDEAIKITNRRVNAIEHVIIP 180

Query: 181 RIENTISYIKGELDELEREDFFRLKKIQGYKK-------REIERQRANAKLYAEEQLAEK 240
           RIE T++YI  ELDE ERE+F+RLKKIQ  KK       +++ER+RA  +          
Sbjct: 181 RIERTLAYIITELDEREREEFYRLKKIQEKKKIIKEKFEKDLERRRAAGE---------- 240

Query: 241 VSLQKGMSISSAHNLLSAAMEKDEDIIF 262
                   +    NLL  A EKDED++F
Sbjct: 241 --------VMEPANLL--AEEKDEDLLF 246

[Close]

BLAST of MS006386 vs. ExPASy Swiss-Prot
Match: Q9Y5K8 (V-type proton ATPase subunit D OS=Homo sapiens OX=9606 GN=ATP6V1D PE=1 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 7.6e-61
Identity = 139/261 (53.26%), Postives = 183/261 (70.11%), Query Frame = 0


Query: 1   MSGQSQRLNVVPTVTMLGAMKARLIGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG 60
           MSG+  R+ + P+      MKARL GA  G  LLKKKSDALT++FRQILKKI+  K  MG
Sbjct: 1   MSGK-DRIEIFPSRMAQTIMKARLKGAQTGRNLLKKKSDALTLRFRQILKKIIETKMLMG 60

Query: 61  EVMKTSSFSLTEAKYVAGDNIKHIVLENVQNAAIKIRSRQENIAGVKLPKFEHYSDGETK 120
           EVM+ ++FSL EAK+ AGD     V++NV  A +KIR++++N+AGV LP FEHY +G   
Sbjct: 61  EVMREAAFSLAEAKFTAGD-FSTTVIQNVNKAQVKIRAKKDNVAGVTLPVFEHYHEGTDS 120

Query: 121 NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 180
            +LTGLARGG+Q+   +  Y KA+E+LVELASLQTSF+TLDEAIK TNRRVNA+E+V+ P
Sbjct: 121 YELTGLARGGEQLAKLKRNYAKAVELLVELASLQTSFVTLDEAIKITNRRVNAIEHVIIP 180

Query: 181 RIENTISYIKGELDELEREDFFRLKKIQGYKKREIERQRANAKLYAEEQLAEKVSLQKGM 240
           RIE T++YI  ELDE ERE+F+RLKKIQ  +K++I ++++   L       E        
Sbjct: 181 RIERTLAYIITELDEREREEFYRLKKIQ--EKKKILKEKSEKDLEQRRAAGE-------- 240

Query: 241 SISSAHNLLSAAMEKDEDIIF 262
            +    NLL  A EKDED++F
Sbjct: 241 -VLEPANLL--AEEKDEDLLF 246

[Close]

BLAST of MS006386 vs. ExPASy Swiss-Prot
Match: Q5RCS8 (V-type proton ATPase subunit D OS=Pongo abelii OX=9601 GN=ATP6V1D PE=2 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 7.6e-61
Identity = 139/261 (53.26%), Postives = 183/261 (70.11%), Query Frame = 0


Query: 1   MSGQSQRLNVVPTVTMLGAMKARLIGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG 60
           MSG+  R+ + P+      MKARL GA  G  LLKKKSDALT++FRQILKKI+  K  MG
Sbjct: 1   MSGK-DRIEIFPSRMAQTIMKARLKGAQTGRNLLKKKSDALTLRFRQILKKIIETKMLMG 60

Query: 61  EVMKTSSFSLTEAKYVAGDNIKHIVLENVQNAAIKIRSRQENIAGVKLPKFEHYSDGETK 120
           EVM+ ++FSL EAK+ AGD     V++NV  A +KIR++++N+AGV LP FEHY +G   
Sbjct: 61  EVMREAAFSLAEAKFTAGD-FSTTVIQNVNKAQVKIRAKKDNVAGVTLPVFEHYHEGTDS 120

Query: 121 NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 180
            +LTGLARGG+Q+   +  Y KA+E+LVELASLQTSF+TLDEAIK TNRRVNA+E+V+ P
Sbjct: 121 YELTGLARGGEQLAKLKRNYAKAVELLVELASLQTSFVTLDEAIKITNRRVNAIEHVIIP 180

Query: 181 RIENTISYIKGELDELEREDFFRLKKIQGYKKREIERQRANAKLYAEEQLAEKVSLQKGM 240
           RIE T++YI  ELDE ERE+F+RLKKIQ  +K++I ++++   L       E        
Sbjct: 181 RIERTLAYIITELDEREREEFYRLKKIQ--EKKKILKEKSEKDLEQRRAAGE-------- 240

Query: 241 SISSAHNLLSAAMEKDEDIIF 262
            +    NLL  A EKDED++F
Sbjct: 241 -VLEPANLL--AEEKDEDLLF 246

[Close]

BLAST of MS006386 vs. ExPASy Swiss-Prot
Match: Q9V7D2 (V-type proton ATPase subunit D 1 OS=Drosophila melanogaster OX=7227 GN=Vha36-1 PE=2 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 1.7e-60
Identity = 139/261 (53.26%), Postives = 185/261 (70.88%), Query Frame = 0


Query: 1   MSGQSQRLNVVPTVTMLGAMKARLIGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG 60
           MSG+  RL + P+      MKARL GA +GH LLKKK+DAL ++FR IL KI+  K  MG
Sbjct: 1   MSGK-DRLPIFPSRGAQMLMKARLAGAQKGHGLLKKKADALQMRFRLILGKIIETKTLMG 60

Query: 61  EVMKTSSFSLTEAKYVAGDNIKHIVLENVQNAAIKIRSRQENIAGVKLPKFEHYSDGETK 120
           +VMK ++FSL EAK+ +GD I  +VL+NV  A IKIR++++N+AGV LP FE Y DG   
Sbjct: 61  DVMKEAAFSLAEAKFTSGD-INQVVLQNVTKAQIKIRTKKDNVAGVTLPVFESYQDGSDT 120

Query: 121 NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 180
            +L GLARGGQQ+   +  Y  A+++LVELASLQTSF+TLDE IK TNRRVNA+E+V+ P
Sbjct: 121 YELAGLARGGQQLAKLKKNYQSAVKLLVELASLQTSFVTLDEVIKITNRRVNAIEHVIIP 180

Query: 181 RIENTISYIKGELDELEREDFFRLKKIQGYKKREIERQRANAKLYAEEQLAEKVSLQKGM 240
           RI+ T++YI  ELDELERE+F+RLKKIQ  KKRE       A++ A+ + AE   LQ+G+
Sbjct: 181 RIDRTLAYIISELDELEREEFYRLKKIQD-KKRE-------ARIKADAKKAE--LLQQGI 240

Query: 241 SISSAHNLLSAAMEKDEDIIF 262
            +    N+L    E D+D++F
Sbjct: 241 DVRQQANILD---EGDDDVLF 246

[Close]

BLAST of MS006386 vs. ExPASy TrEMBL
Match: A0A6J1D312 (V-type proton ATPase subunit D OS=Momordica charantia OX=3673 GN=LOC111016833 PE=3 SV=1)

HSP 1 Score: 485.0 bits (1247), Expect = 2.0e-133
Identity = 261/261 (100.00%), Postives = 261/261 (100.00%), Query Frame = 0


Query: 1   MSGQSQRLNVVPTVTMLGAMKARLIGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG 60
           MSGQSQRLNVVPTVTMLGAMKARLIGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG
Sbjct: 1   MSGQSQRLNVVPTVTMLGAMKARLIGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG 60

Query: 61  EVMKTSSFSLTEAKYVAGDNIKHIVLENVQNAAIKIRSRQENIAGVKLPKFEHYSDGETK 120
           EVMKTSSFSLTEAKYVAGDNIKHIVLENVQNAAIKIRSRQENIAGVKLPKFEHYSDGETK
Sbjct: 61  EVMKTSSFSLTEAKYVAGDNIKHIVLENVQNAAIKIRSRQENIAGVKLPKFEHYSDGETK 120

Query: 121 NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 180
           NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP
Sbjct: 121 NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 180

Query: 181 RIENTISYIKGELDELEREDFFRLKKIQGYKKREIERQRANAKLYAEEQLAEKVSLQKGM 240
           RIENTISYIKGELDELEREDFFRLKKIQGYKKREIERQRANAKLYAEEQLAEKVSLQKGM
Sbjct: 181 RIENTISYIKGELDELEREDFFRLKKIQGYKKREIERQRANAKLYAEEQLAEKVSLQKGM 240

Query: 241 SISSAHNLLSAAMEKDEDIIF 262
           SISSAHNLLSAAMEKDEDIIF
Sbjct: 241 SISSAHNLLSAAMEKDEDIIF 261

[Close]

BLAST of MS006386 vs. ExPASy TrEMBL
Match: A0A6J1L2U2 (V-type proton ATPase subunit D-like OS=Cucurbita maxima OX=3661 GN=LOC111499899 PE=3 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 1.0e-129
Identity = 254/261 (97.32%), Postives = 260/261 (99.62%), Query Frame = 0


Query: 1   MSGQSQRLNVVPTVTMLGAMKARLIGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG 60
           MSGQ+QRLNVVPTVTMLGAMKARLIGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG
Sbjct: 1   MSGQTQRLNVVPTVTMLGAMKARLIGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG 60

Query: 61  EVMKTSSFSLTEAKYVAGDNIKHIVLENVQNAAIKIRSRQENIAGVKLPKFEHYSDGETK 120
           EVMKTSSFSLTEAKYVAGDNIKHIVLENVQNAAIKIRSRQ+NIAGVKLPKFEHYSDGETK
Sbjct: 61  EVMKTSSFSLTEAKYVAGDNIKHIVLENVQNAAIKIRSRQDNIAGVKLPKFEHYSDGETK 120

Query: 121 NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 180
           NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP
Sbjct: 121 NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 180

Query: 181 RIENTISYIKGELDELEREDFFRLKKIQGYKKREIERQRANAKLYAEEQLAEKVSLQKGM 240
           RIENTISYIKGELDELEREDFFRLKKIQGYK+REIERQRANAKLYAEEQLAEKVSLQKG+
Sbjct: 181 RIENTISYIKGELDELEREDFFRLKKIQGYKRREIERQRANAKLYAEEQLAEKVSLQKGV 240

Query: 241 SISSAHNLLSAAMEKDEDIIF 262
           SISSA++LLSAA EKDEDIIF
Sbjct: 241 SISSAYSLLSAAGEKDEDIIF 261

[Close]

BLAST of MS006386 vs. ExPASy TrEMBL
Match: A0A6J1G8R8 (V-type proton ATPase subunit D-like OS=Cucurbita moschata OX=3662 GN=LOC111451762 PE=3 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 1.0e-129
Identity = 254/261 (97.32%), Postives = 260/261 (99.62%), Query Frame = 0


Query: 1   MSGQSQRLNVVPTVTMLGAMKARLIGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG 60
           MSGQ+QRLNVVPTVTMLGAMKARLIGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG
Sbjct: 1   MSGQTQRLNVVPTVTMLGAMKARLIGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG 60

Query: 61  EVMKTSSFSLTEAKYVAGDNIKHIVLENVQNAAIKIRSRQENIAGVKLPKFEHYSDGETK 120
           EVMKTSSFSLTEAKYVAGDNIKHIVLENVQNAAIKIRSRQ+NIAGVKLPKFEHYSDGETK
Sbjct: 61  EVMKTSSFSLTEAKYVAGDNIKHIVLENVQNAAIKIRSRQDNIAGVKLPKFEHYSDGETK 120

Query: 121 NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 180
           NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP
Sbjct: 121 NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 180

Query: 181 RIENTISYIKGELDELEREDFFRLKKIQGYKKREIERQRANAKLYAEEQLAEKVSLQKGM 240
           RIENTISYIKGELDELEREDFFRLKKIQGYK+REIERQRANAKLYAEEQLAEKVSLQKG+
Sbjct: 181 RIENTISYIKGELDELEREDFFRLKKIQGYKRREIERQRANAKLYAEEQLAEKVSLQKGV 240

Query: 241 SISSAHNLLSAAMEKDEDIIF 262
           SISSA++LLSAA EKDEDIIF
Sbjct: 241 SISSAYSLLSAAGEKDEDIIF 261

[Close]

BLAST of MS006386 vs. ExPASy TrEMBL
Match: A0A0A0KGM2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G422820 PE=3 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 1.4e-129
Identity = 252/261 (96.55%), Postives = 259/261 (99.23%), Query Frame = 0


Query: 1   MSGQSQRLNVVPTVTMLGAMKARLIGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG 60
           MSGQ+QRLNVVPTVTMLGAMKARL+GATRGHALLKKKSDALTVQFRQILKKIVSAKESMG
Sbjct: 1   MSGQTQRLNVVPTVTMLGAMKARLVGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG 60

Query: 61  EVMKTSSFSLTEAKYVAGDNIKHIVLENVQNAAIKIRSRQENIAGVKLPKFEHYSDGETK 120
           +VMKTS+FSLTEAKYVAGDNIKHIVLENVQ AAIKIRSRQENIAGVKLPKFEHYSDGETK
Sbjct: 61  DVMKTSAFSLTEAKYVAGDNIKHIVLENVQTAAIKIRSRQENIAGVKLPKFEHYSDGETK 120

Query: 121 NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 180
           NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP
Sbjct: 121 NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 180

Query: 181 RIENTISYIKGELDELEREDFFRLKKIQGYKKREIERQRANAKLYAEEQLAEKVSLQKGM 240
           RIENTISYIKGELDELEREDFFRLKKIQGYK+REIERQRANAKL+AEEQLAEKVSLQKG+
Sbjct: 181 RIENTISYIKGELDELEREDFFRLKKIQGYKRREIERQRANAKLFAEEQLAEKVSLQKGV 240

Query: 241 SISSAHNLLSAAMEKDEDIIF 262
           SISSAHNLLSAA EKDEDIIF
Sbjct: 241 SISSAHNLLSAAAEKDEDIIF 261

[Close]

BLAST of MS006386 vs. ExPASy TrEMBL
Match: A0A6J1JNF5 (V-type proton ATPase subunit D-like OS=Cucurbita maxima OX=3661 GN=LOC111486278 PE=3 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 4.0e-129
Identity = 250/261 (95.79%), Postives = 259/261 (99.23%), Query Frame = 0


Query: 1   MSGQSQRLNVVPTVTMLGAMKARLIGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG 60
           MSGQ+QRLNVVPTVTMLGAMKARL+GATRGHALLKKKSDALTVQFRQILKKIVSAKESMG
Sbjct: 1   MSGQTQRLNVVPTVTMLGAMKARLVGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG 60

Query: 61  EVMKTSSFSLTEAKYVAGDNIKHIVLENVQNAAIKIRSRQENIAGVKLPKFEHYSDGETK 120
           EVMKTS+FSLTEAKYVAGDNIKHIVLENVQ+AAIKIRSRQENIAGVKLPKFEHYSDGETK
Sbjct: 61  EVMKTSAFSLTEAKYVAGDNIKHIVLENVQSAAIKIRSRQENIAGVKLPKFEHYSDGETK 120

Query: 121 NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 180
           NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP
Sbjct: 121 NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 180

Query: 181 RIENTISYIKGELDELEREDFFRLKKIQGYKKREIERQRANAKLYAEEQLAEKVSLQKGM 240
           RIENTISYIKGELDELEREDFFRLKKIQGYK+REIERQR NAKLYAEEQLAEK+SLQKG+
Sbjct: 181 RIENTISYIKGELDELEREDFFRLKKIQGYKRREIERQRINAKLYAEEQLAEKISLQKGV 240

Query: 241 SISSAHNLLSAAMEKDEDIIF 262
           S++SAHNLLSAA EKDEDIIF
Sbjct: 241 SMTSAHNLLSAAAEKDEDIIF 261

[Close]

BLAST of MS006386 vs. TAIR 10
Match: AT3G58730.1 (vacuolar ATP synthase subunit D (VATD) / V-ATPase D subunit / vacuolar proton pump D subunit (VATPD) )

HSP 1 Score: 410.2 bits (1053), Expect = 1.2e-114
Identity = 210/261 (80.46%), Postives = 240/261 (91.95%), Query Frame = 0


Query: 1   MSGQSQRLNVVPTVTMLGAMKARLIGATRGHALLKKKSDALTVQFRQILKKIVSAKESMG 60
           M+GQ+ RLNVVPTVTMLG MKARL+GATRGHALLKKKSDALTVQFR +LKKIV+AKESMG
Sbjct: 1   MAGQNARLNVVPTVTMLGVMKARLVGATRGHALLKKKSDALTVQFRALLKKIVTAKESMG 60

Query: 61  EVMKTSSFSLTEAKYVAGDNIKHIVLENVQNAAIKIRSRQENIAGVKLPKFEHYSDGETK 120
           ++MKTSSF+LTE KYVAGDN+KH+VLENV+ A +K+RSR ENIAGVKLPKF+H+S+GETK
Sbjct: 61  DMMKTSSFALTEVKYVAGDNVKHVVLENVKEATLKVRSRTENIAGVKLPKFDHFSEGETK 120

Query: 121 NDLTGLARGGQQIQLCRGAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 180
           NDLTGLARGGQQ++ CR AYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP
Sbjct: 121 NDLTGLARGGQQVRACRVAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKP 180

Query: 181 RIENTISYIKGELDELEREDFFRLKKIQGYKKREIERQRANAKLYAEEQLAEKVSLQKGM 240
           ++ENTISYIKGELDELEREDFFRLKKIQGYK+RE+ERQ ANAK +AEE + E +S+Q+G+
Sbjct: 181 KLENTISYIKGELDELEREDFFRLKKIQGYKRREVERQAANAKEFAEEMVLEDISMQRGI 240

Query: 241 SISSAHNLLSAAMEKDEDIIF 262
           SI++A N L    EKD DIIF
Sbjct: 241 SINAARNFLVGGAEKDSDIIF 261

The following BLAST results are available for this feature:

BLAST of MS006386 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148058.1	4.2e-133	100.00	V-type proton ATPase subunit D [Momordica charantia]	[more]
XP_022948069.1	2.2e-129	97.32	V-type proton ATPase subunit D-like [Cucurbita moschata] >XP_023007390.1 V-type ...	[more]
KGN48252.2	2.8e-129	96.55	hypothetical protein Csa_003783 [Cucumis sativus]	[more]
XP_004140139.1	2.8e-129	96.55	V-type proton ATPase subunit D [Cucumis sativus] >XP_031743924.1 V-type proton A...	[more]
KAG6589288.1	8.2e-129	95.79	Glutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial, part...	[more]

BLAST of MS006386 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XGM1	1.7e-113	80.46	V-type proton ATPase subunit D OS=Arabidopsis thaliana OX=3702 GN=VHA-D PE=1 SV=...	[more]
P57746	4.5e-61	52.61	V-type proton ATPase subunit D OS=Mus musculus OX=10090 GN=Atp6v1d PE=1 SV=1	[more]
Q9Y5K8	7.6e-61	53.26	V-type proton ATPase subunit D OS=Homo sapiens OX=9606 GN=ATP6V1D PE=1 SV=1	[more]
Q5RCS8	7.6e-61	53.26	V-type proton ATPase subunit D OS=Pongo abelii OX=9601 GN=ATP6V1D PE=2 SV=1	[more]
Q9V7D2	1.7e-60	53.26	V-type proton ATPase subunit D 1 OS=Drosophila melanogaster OX=7227 GN=Vha36-1 P...	[more]

BLAST of MS006386 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D312	2.0e-133	100.00	V-type proton ATPase subunit D OS=Momordica charantia OX=3673 GN=LOC111016833 PE...	[more]
A0A6J1L2U2	1.0e-129	97.32	V-type proton ATPase subunit D-like OS=Cucurbita maxima OX=3661 GN=LOC111499899 ...	[more]
A0A6J1G8R8	1.0e-129	97.32	V-type proton ATPase subunit D-like OS=Cucurbita moschata OX=3662 GN=LOC11145176...	[more]
A0A0A0KGM2	1.4e-129	96.55	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G422820 PE=3 SV=1	[more]
A0A6J1JNF5	4.0e-129	95.79	V-type proton ATPase subunit D-like OS=Cucurbita maxima OX=3661 GN=LOC111486278 ...	[more]

BLAST of MS006386 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G58730.1	1.2e-114	80.46	vacuolar ATP synthase subunit D (VATD) / V-ATPase D subunit / vacuolar proton pu...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002699	ATPase, V1 complex, subunit D	TIGRFAM	TIGR00309	TIGR00309	coord: 9..212 e-value: 3.3E-60 score: 201.2
IPR002699	ATPase, V1 complex, subunit D	PFAM	PF01813	ATP-synt_D	coord: 17..208 e-value: 2.9E-72 score: 242.6
IPR002699	ATPase, V1 complex, subunit D	PANTHER	PTHR11671	V-TYPE ATP SYNTHASE SUBUNIT D	coord: 1..261
None	No IPR available	GENE3D	1.10.287.3240		coord: 1..198 e-value: 1.2E-74 score: 252.2
None	No IPR available	PANTHER	PTHR11671:SF3	V-TYPE PROTON ATPASE SUBUNIT D-RELATED	coord: 1..261

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS006386.1	MS006386.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0042626	ATPase-coupled transmembrane transporter activity