MS006381 (gene) Bitter gourd (TR) v1

Overview
NameMS006381
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionSHSP domain-containing protein
Locationscaffold327: 225854 .. 227786 (+)
RNA-Seq ExpressionMS006381
SyntenyMS006381
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAACCATGCCGGAAAACGCAGACTCGACATGATCGCCGCCCACCTTGCTCCTGCCGGTGATATTTCCGCTGCTCACGCACACGTTCTCCCCGCGGTACTCTTCTGTTTATCTTTCTTTCATTAAATCCGGCGAACCGTTTTCTTTTGTGGGTTTAGAATTAGATCGAGCGGGGTTATTGCAGAAATTTCTGCTGTACACTTCATTTCATCTTTCCCGGTGCTCATTTTGTTAGTTTCTGGATTGGATTTGCTCGATCGATCATCGTTTTTTTGGTTTTGGGTGATTAGAATTGCAGTAGCTATGGAAGCTCTGTCATTCGAAGGTGCGATAATAGGATGTTCTTTGCGCGGCAAGGATCCGAGTCTTGTGGTGCTTATATGAGGCAGGCTTCGATTACAGAGGTGCTTTATGTTAAGAAACTAAATTTACCTAATCCATTTTTGATTTGACATATCATATTTCTTTCATTATATTTTACTTTCTATTTATGGAGACCTGATTTTTTTTTTCTTTTCCTCTGACTTCTTTTTTGAAGAATATATATCAGGGTAATCAAGGATCTAATTCTCAAGCGTCCCTGGATGCTTATGAAGGACCATTATATTCAAGACCTGCAACGGAGGGAAGGATTCCGGGTGTTGGAATAGAACAGCCCGTGGCACAGAATTTCAATTTAATTGCGTCTGAACCTCCGTTGTTTGCCAGGGCATGCTTTGGAATCAGTGAACATAACAACCATTTCGTTTCGGAGACAAAGCAGCCATCCTCTGGATCAAATGGTTGGTTATTGATTTCCCAATTGATAATTGATAAACGAGTATAAAGCACCTTGGCAGGCCCTTGGCCACTGAGATTTTACTTCTTCCTTTTTAATGAGAATCGAAAGTGCTTTAAAACTTACTTCCACTGTTCTTATTTAACACAGGAATTGGTGGATGTTCTCCGAGGATGGATGTGGCAGAATCAGGTAGAGGTTATGTACTAACCGTGGAAATTCCAGGAGTCAAGATCAATGACATCAGAGTTGAGGTCGATGATCAAAAGTAAGACAATGTTATTTTATAAAGATACTTTAAAATTTGGAGTTGTAAATGTAGTATTCAGATCTTGTACCTTTTCAACTATTAATCCTCCCCAATGTTCAGAACTATCTGATTCAACTGCATATTCTTAAGTCTCCCTGAAAGTTACACTAAAAAGCATTTGAAATCTTAATTTCCATGTGCTAAATTATCACTAGATTTACAAGCTATTGTAATTTTATTCATTTATTTATTTTCAACACACTGGATTTGGATTTAAATATTGAAGTAAAAATGACACTAGTCAGGATGTCCTACTGATCACAATGTTTAAGTTAACCCTAAGTTCATAGGTTGTTCGATATCATCAAACTCAAGCTAAAGTTCAAGCTATTAGATGAAGATAAGAATAAGCTTGAATGTTAGCATGAGACCTATAGGCTGTACATGGACTACTGATATTATAGGAATGGAAATGGGGTGGATTTAAGTTATGCCCCTACCGTCCTCAGCCCCGTGGGTTATTTTAATCATTTCTTAAGAAAAAATCCCTGTTCTAGACGAAATTAGGATGTTAGGGAACTTGTCATCCTAACAAAATACGGAAGAAACAATTCAAATTCAATAGTTTCTATATGATATATAGCTTGCTAAATAACTACTGGATCATAAAACATAAGTAAGTTTAAATGACCATATAATGGCATTGATATGATGGTGAAATGCTGTTCTCTTGCAGGTTGACCATAATCGGCAAACGCTCAAATCAATACCATGAAATGGTAGGATATTCAAGAGAATCTATCCGTTCATATCACAAGAGAGAGATTTTACAAGGACCTTACCAAGTGGTGTGGCCACTTCCGATCAACATTAACAAGGACGGAGTATCAGCTGAGTTCTGG

mRNA sequence

ATGGAGAACCATGCCGGAAAACGCAGACTCGACATGATCGCCGCCCACCTTGCTCCTGCCGGTGATATTTCCGCTGCTCACGCACACGTTCTCCCCGCGAATTGCAGTAGCTATGGAAGCTCTGTCATTCGAAGGTGCGATAATAGGATGTTCTTTGCGCGGCAAGGATCCGAGTCTTGTGGTGCTTATATGAGGCAGGCTTCGATTACAGAGGGTAATCAAGGATCTAATTCTCAAGCGTCCCTGGATGCTTATGAAGGACCATTATATTCAAGACCTGCAACGGAGGGAAGGATTCCGGGTGTTGGAATAGAACAGCCCGTGGCACAGAATTTCAATTTAATTGCGTCTGAACCTCCGTTGTTTGCCAGGGCATGCTTTGGAATCAGTGAACATAACAACCATTTCGTTTCGGAGACAAAGCAGCCATCCTCTGGATCAAATGGAATTGGTGGATGTTCTCCGAGGATGGATGTGGCAGAATCAGGTAGAGGTTATGTACTAACCGTGGAAATTCCAGGAGTCAAGATCAATGACATCAGAGTTGAGGTCGATGATCAAAAGTTGACCATAATCGGCAAACGCTCAAATCAATACCATGAAATGGTAGGATATTCAAGAGAATCTATCCGTTCATATCACAAGAGAGAGATTTTACAAGGACCTTACCAAGTGGTGTGGCCACTTCCGATCAACATTAACAAGGACGGAGTATCAGCTGAGTTCTGG

Coding sequence (CDS)

ATGGAGAACCATGCCGGAAAACGCAGACTCGACATGATCGCCGCCCACCTTGCTCCTGCCGGTGATATTTCCGCTGCTCACGCACACGTTCTCCCCGCGAATTGCAGTAGCTATGGAAGCTCTGTCATTCGAAGGTGCGATAATAGGATGTTCTTTGCGCGGCAAGGATCCGAGTCTTGTGGTGCTTATATGAGGCAGGCTTCGATTACAGAGGGTAATCAAGGATCTAATTCTCAAGCGTCCCTGGATGCTTATGAAGGACCATTATATTCAAGACCTGCAACGGAGGGAAGGATTCCGGGTGTTGGAATAGAACAGCCCGTGGCACAGAATTTCAATTTAATTGCGTCTGAACCTCCGTTGTTTGCCAGGGCATGCTTTGGAATCAGTGAACATAACAACCATTTCGTTTCGGAGACAAAGCAGCCATCCTCTGGATCAAATGGAATTGGTGGATGTTCTCCGAGGATGGATGTGGCAGAATCAGGTAGAGGTTATGTACTAACCGTGGAAATTCCAGGAGTCAAGATCAATGACATCAGAGTTGAGGTCGATGATCAAAAGTTGACCATAATCGGCAAACGCTCAAATCAATACCATGAAATGGTAGGATATTCAAGAGAATCTATCCGTTCATATCACAAGAGAGAGATTTTACAAGGACCTTACCAAGTGGTGTGGCCACTTCCGATCAACATTAACAAGGACGGAGTATCAGCTGAGTTCTGG

Protein sequence

MENHAGKRRLDMIAAHLAPAGDISAAHAHVLPANCSSYGSSVIRRCDNRMFFARQGSESCGAYMRQASITEGNQGSNSQASLDAYEGPLYSRPATEGRIPGVGIEQPVAQNFNLIASEPPLFARACFGISEHNNHFVSETKQPSSGSNGIGGCSPRMDVAESGRGYVLTVEIPGVKINDIRVEVDDQKLTIIGKRSNQYHEMVGYSRESIRSYHKREILQGPYQVVWPLPININKDGVSAEFW
Homology
BLAST of MS006381 vs. NCBI nr
Match: XP_022148040.1 (uncharacterized protein LOC111016826 isoform X2 [Momordica charantia])

HSP 1 Score: 490.0 bits (1260), Expect = 1.2e-134
Identity = 241/243 (99.18%), Postives = 241/243 (99.18%), Query Frame = 0

Query: 1   MENHAGKRRLDMIAAHLAPAGDISAAHAHVLPANCSSYGSSVIRRCDNRMFFARQGSESC 60
           MENHAGKRRLDMIAAHLAPAGDISAAHAHVLPANCSSYGSSVIRRCDNRMFFARQGSESC
Sbjct: 1   MENHAGKRRLDMIAAHLAPAGDISAAHAHVLPANCSSYGSSVIRRCDNRMFFARQGSESC 60

Query: 61  GAYMRQASITEGNQGSNSQASLDAYEGPLYSRPATEGRIPGVGIEQPVAQNFNLIASEPP 120
           GAYMRQASITEGNQ SNSQASLDAYEGPLYSRPATEGRIPGVGIEQPVAQNFNLIASEPP
Sbjct: 61  GAYMRQASITEGNQRSNSQASLDAYEGPLYSRPATEGRIPGVGIEQPVAQNFNLIASEPP 120

Query: 121 LFARACFGISEHNNHFVSETKQPSSGSNGIGGCSPRMDVAESGRGYVLTVEIPGVKINDI 180
           LFARACFGISEHNNHFVSETKQPSSGSNGIGGCSPRMDVAESGRGYVLTVEIPGVKINDI
Sbjct: 121 LFARACFGISEHNNHFVSETKQPSSGSNGIGGCSPRMDVAESGRGYVLTVEIPGVKINDI 180

Query: 181 RVEVDDQKLTIIGKRSNQYHEMVGYSRESIRSYHKREILQGPYQVVWPLPININKDGVSA 240
           RVEVDDQKLTIIGKRSNQYHEMVG SRESIRSYHKREILQGPYQVVWPLPININKDGVSA
Sbjct: 181 RVEVDDQKLTIIGKRSNQYHEMVGCSRESIRSYHKREILQGPYQVVWPLPININKDGVSA 240

Query: 241 EFW 244
           EFW
Sbjct: 241 EFW 243

BLAST of MS006381 vs. NCBI nr
Match: XP_022148039.1 (uncharacterized protein LOC111016826 isoform X1 [Momordica charantia])

HSP 1 Score: 484.2 bits (1245), Expect = 6.7e-133
Identity = 241/247 (97.57%), Postives = 241/247 (97.57%), Query Frame = 0

Query: 1   MENHAGKRRLDMIAAHLAPAGDISAAHAHVLPANCSSYGSSVIRRCDNRMFFARQGSESC 60
           MENHAGKRRLDMIAAHLAPAGDISAAHAHVLPANCSSYGSSVIRRCDNRMFFARQGSESC
Sbjct: 1   MENHAGKRRLDMIAAHLAPAGDISAAHAHVLPANCSSYGSSVIRRCDNRMFFARQGSESC 60

Query: 61  GAYMRQASITE----GNQGSNSQASLDAYEGPLYSRPATEGRIPGVGIEQPVAQNFNLIA 120
           GAYMRQASITE    GNQ SNSQASLDAYEGPLYSRPATEGRIPGVGIEQPVAQNFNLIA
Sbjct: 61  GAYMRQASITENIYQGNQRSNSQASLDAYEGPLYSRPATEGRIPGVGIEQPVAQNFNLIA 120

Query: 121 SEPPLFARACFGISEHNNHFVSETKQPSSGSNGIGGCSPRMDVAESGRGYVLTVEIPGVK 180
           SEPPLFARACFGISEHNNHFVSETKQPSSGSNGIGGCSPRMDVAESGRGYVLTVEIPGVK
Sbjct: 121 SEPPLFARACFGISEHNNHFVSETKQPSSGSNGIGGCSPRMDVAESGRGYVLTVEIPGVK 180

Query: 181 INDIRVEVDDQKLTIIGKRSNQYHEMVGYSRESIRSYHKREILQGPYQVVWPLPININKD 240
           INDIRVEVDDQKLTIIGKRSNQYHEMVG SRESIRSYHKREILQGPYQVVWPLPININKD
Sbjct: 181 INDIRVEVDDQKLTIIGKRSNQYHEMVGCSRESIRSYHKREILQGPYQVVWPLPININKD 240

Query: 241 GVSAEFW 244
           GVSAEFW
Sbjct: 241 GVSAEFW 247

BLAST of MS006381 vs. NCBI nr
Match: ADU55782.1 (HSP27.8 [Citrullus lanatus])

HSP 1 Score: 428.7 bits (1101), Expect = 3.3e-116
Identity = 210/243 (86.42%), Postives = 222/243 (91.36%), Query Frame = 0

Query: 1   MENHAGKRRLDMIAAHLAPAGDISAAHAHVLPANCSSYGSSVIRRCDNRMFFARQGSESC 60
           ME+HAGKRRLD++ AHLAPAGDIS A+ HVLPANC SY SSVIRRCDN+ +FARQGSE+C
Sbjct: 1   MEHHAGKRRLDVLTAHLAPAGDISPANGHVLPANCCSYESSVIRRCDNKTYFARQGSEAC 60

Query: 61  GAYMRQASITEGNQGSNSQASLDAYEGPLYSRPATEGRIPGVGIEQPVAQNFNLIASEPP 120
           GAYMRQAS TEGNQ SNSQA  D  EGPLYSRPA EG+IP VGIEQPVAQNFNLIASEPP
Sbjct: 61  GAYMRQASTTEGNQRSNSQAFRDVSEGPLYSRPAKEGKIPSVGIEQPVAQNFNLIASEPP 120

Query: 121 LFARACFGISEHNNHFVSETKQPSSGSNGIGGCSPRMDVAESGRGYVLTVEIPGVKINDI 180
           LFARAC GI+E NNHF+SETKQPSSGSNGIGG SPRMDVAESGRGYVLTVEIPGVKINDI
Sbjct: 121 LFARACLGINEQNNHFISETKQPSSGSNGIGGLSPRMDVAESGRGYVLTVEIPGVKINDI 180

Query: 181 RVEVDDQKLTIIGKRSNQYHEMVGYSRESIRSYHKREILQGPYQVVWPLPININKDGVSA 240
           RVEVDDQKLTIIGKRSNQY E+VGYS +SI SYHKREILQGPYQVVWPLPININKDGV A
Sbjct: 181 RVEVDDQKLTIIGKRSNQYCEVVGYSSDSISSYHKREILQGPYQVVWPLPININKDGVFA 240

Query: 241 EFW 244
           EFW
Sbjct: 241 EFW 243

BLAST of MS006381 vs. NCBI nr
Match: XP_038886846.1 (uncharacterized protein LOC120077066 [Benincasa hispida])

HSP 1 Score: 421.8 bits (1083), Expect = 4.1e-114
Identity = 208/243 (85.60%), Postives = 219/243 (90.12%), Query Frame = 0

Query: 1   MENHAGKRRLDMIAAHLAPAGDISAAHAHVLPANCSSYGSSVIRRCDNRMFFARQGSESC 60
           ME+HAGKRRLD++AAHLAPAGDIS AH HVLPANC    SSVIRRCDN+ +FARQGSE+C
Sbjct: 1   MEHHAGKRRLDVLAAHLAPAGDISPAHGHVLPANC----SSVIRRCDNKTYFARQGSEAC 60

Query: 61  GAYMRQASITEGNQGSNSQASLDAYEGPLYSRPATEGRIPGVGIEQPVAQNFNLIASEPP 120
           GAYMRQ S TEGNQ SNSQA  D  EGPLYSRPA EGRIP VG+EQPVAQNFNLIASEPP
Sbjct: 61  GAYMRQGSTTEGNQRSNSQAFWDVSEGPLYSRPAEEGRIPSVGMEQPVAQNFNLIASEPP 120

Query: 121 LFARACFGISEHNNHFVSETKQPSSGSNGIGGCSPRMDVAESGRGYVLTVEIPGVKINDI 180
           LFARAC GI+EHNNHF+SE KQPSSGSNGIGG SPRMDVAESGRGYVLTVEIPGVKINDI
Sbjct: 121 LFARACLGINEHNNHFISEIKQPSSGSNGIGGLSPRMDVAESGRGYVLTVEIPGVKINDI 180

Query: 181 RVEVDDQKLTIIGKRSNQYHEMVGYSRESIRSYHKREILQGPYQVVWPLPININKDGVSA 240
           RVEVDDQKLTIIGKRSNQY E+VGYS +SI SYHKREILQGPYQVVWPLP NINKDGV A
Sbjct: 181 RVEVDDQKLTIIGKRSNQYCEVVGYSSDSISSYHKREILQGPYQVVWPLPTNINKDGVLA 239

Query: 241 EFW 244
           EFW
Sbjct: 241 EFW 239

BLAST of MS006381 vs. NCBI nr
Match: XP_022953065.1 (uncharacterized protein LOC111455581 isoform X2 [Cucurbita moschata])

HSP 1 Score: 402.5 bits (1033), Expect = 2.6e-108
Identity = 199/245 (81.22%), Postives = 214/245 (87.35%), Query Frame = 0

Query: 1   MENHAGKRRLDMIAAHLAPAGDISAAHAHVLPANCSSYGSSVIRRCDNRMFFARQGSESC 60
           ME+HAGKRRLD++AAHLAPAGDIS    HVLPANC SYGSSVIRR DN+ +FARQGSE+C
Sbjct: 1   MEHHAGKRRLDVLAAHLAPAGDISTGQGHVLPANCCSYGSSVIRRFDNKTYFARQGSEAC 60

Query: 61  GAYMRQASITE--GNQGSNSQASLDAYEGPLYSRPATEGRIPGVGIEQPVAQNFNLIASE 120
           GAYMRQAS TE  GNQ SNSQ  LD  EGP+YSRPA +G IP +GIEQ + QNFNLIASE
Sbjct: 61  GAYMRQASATEVQGNQRSNSQPFLDVSEGPVYSRPAKDGGIPSIGIEQLMTQNFNLIASE 120

Query: 121 PPLFARACFGISEHNNHFVSETKQPSSGSNGIGGCSPRMDVAESGRGYVLTVEIPGVKIN 180
           PPLFARAC GI+E NNH +SE KQPSSGS G+GG SPRMDVAESGRGYVLTVEIPGVKIN
Sbjct: 121 PPLFARACLGINEQNNHVISEAKQPSSGSKGVGGLSPRMDVAESGRGYVLTVEIPGVKIN 180

Query: 181 DIRVEVDDQKLTIIGKRSNQYHEMVGYSRESIRSYHKREILQGPYQVVWPLPININKDGV 240
           DIRVEVDDQKLTIIGKRSNQY E+VGYS +SI SYHKREI QGPYQVVWPLPININKDGV
Sbjct: 181 DIRVEVDDQKLTIIGKRSNQYCEVVGYSSDSISSYHKREISQGPYQVVWPLPININKDGV 240

Query: 241 SAEFW 244
            AEFW
Sbjct: 241 LAEFW 245

BLAST of MS006381 vs. ExPASy Swiss-Prot
Match: Q4UKR8 (Small heat shock protein C2 OS=Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) OX=315456 GN=hspC2 PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.1e-10
Identity = 34/88 (38.64%), Postives = 50/88 (56.82%), Query Frame = 0

Query: 155 PRMDVAESGRGYVLTVEIPGVKINDIRVEVDDQKLTIIGKRSNQYHEMVGYSRESIRSYH 214
           PR D++E+  GY L VE+PG+   DI + +D+  LTI G++  +       S E  ++YH
Sbjct: 50  PRTDISETDSGYSLEVELPGINQKDIDINIDNHILTIKGQKEEK-------SEEKNKNYH 109

Query: 215 KREILQGPYQVVWPLPININKDGVSAEF 243
            RE   G +Q    LP NIN D ++A F
Sbjct: 110 MRERYYGSFQRSISLPANINDDAINARF 130

BLAST of MS006381 vs. ExPASy Swiss-Prot
Match: Q4UJB1 (Small heat shock protein C4 OS=Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) OX=315456 GN=hspc4-1 PE=3 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 1.0e-06
Identity = 35/112 (31.25%), Postives = 58/112 (51.79%), Query Frame = 0

Query: 133 NNHF--VSETKQPSSGSNGIGGCSPRMDVAESGRGYVLTVEIPGVKINDIRVEVDDQKLT 192
           NN F  ++    P+  +N I   SPR D+ E+   Y L VE+PGV  ++I +++D   LT
Sbjct: 38  NNFFNEIASFSYPTHYNNKI--LSPRTDITENESEYHLEVELPGVTQDNIDLKIDSNILT 97

Query: 193 IIGKRSNQYHEMVGYSRESIRSYHKREILQGPYQVVWPLPININKDGVSAEF 243
           I GK+          + +   +YH +E   G +     LP N++++ V+A F
Sbjct: 98  IDGKKEQS-------TEKKDHNYHMKERYYGSFSRSISLPSNVDEEHVTANF 140

BLAST of MS006381 vs. ExPASy Swiss-Prot
Match: Q9XIE3 (17.6 kDa class I heat shock protein 1 OS=Arabidopsis thaliana OX=3702 GN=HSP17.6A PE=1 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 4.7e-04
Identity = 31/103 (30.10%), Postives = 52/103 (50.49%), Query Frame = 0

Query: 139 ETKQPSSGSNGIGGCSPRMDVAESGRGYVLTVEIPGVKINDIRVEV-DDQKLTIIGKRSN 198
           E + PSS S+ I   + R+D  E+   +V   ++PG+K  +++VE+ DD  L I G+R  
Sbjct: 32  ELQFPSSSSSAI--ANARVDWKETAEAHVFKADLPGMKKEEVKVEIEDDSVLKISGERHV 91

Query: 199 QYHEMVGYSRESIRSYHKREILQGPYQVVWPLPININKDGVSA 241
           +         E   ++H+ E   G +   + LP N+  D V A
Sbjct: 92  E-------KEEKQDTWHRVERSSGGFSRKFRLPENVKMDQVKA 125

BLAST of MS006381 vs. ExPASy Swiss-Prot
Match: P46516 (17.9 kDa class II heat shock protein OS=Helianthus annuus OX=4232 GN=HSP17.9 PE=2 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 4.7e-04
Identity = 26/83 (31.33%), Postives = 41/83 (49.40%), Query Frame = 0

Query: 158 DVAESGRGYVLTVEIPGVKINDIRVEVDDQKLTIIGKRSNQYHEMVGYSRESIRSYHKRE 217
           DV E    YV  V++PG+K  DI+V+V+   + +I  + N+  E  G        Y + E
Sbjct: 54  DVKECPNSYVFIVDMPGLKSGDIKVQVERDNVLVISGKRNREEEKEGV------KYVRME 113

Query: 218 ILQGPYQVVWPLPININKDGVSA 241
              G +   + LP + N D +SA
Sbjct: 114 RRMGKFMKKFALPEDANTDKISA 130

BLAST of MS006381 vs. ExPASy Swiss-Prot
Match: Q01544 (17.2 kDa class II heat shock protein OS=Ipomoea nil OX=35883 GN=SHSP-1 PE=2 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 4.7e-04
Identity = 29/84 (34.52%), Postives = 43/84 (51.19%), Query Frame = 0

Query: 158 DVAESGRGYVLTVEIPGVKINDIRVEVD-DQKLTIIGKRSNQYHEMVGYSRESIRSYHKR 217
           DV E    YV  +++PG+K  DI+V+VD D  L+I G+R  +  E  G        Y + 
Sbjct: 48  DVKEYPNSYVFIIDMPGLKSGDIKVQVDGDNVLSISGERKREAEEKEG------AKYVRM 107

Query: 218 EILQGPYQVVWPLPININKDGVSA 241
           E   G     + LP N NK+ ++A
Sbjct: 108 ERRVGKLMRKFVLPENANKEKITA 125

BLAST of MS006381 vs. ExPASy TrEMBL
Match: A0A6J1D1T8 (uncharacterized protein LOC111016826 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016826 PE=3 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 5.9e-135
Identity = 241/243 (99.18%), Postives = 241/243 (99.18%), Query Frame = 0

Query: 1   MENHAGKRRLDMIAAHLAPAGDISAAHAHVLPANCSSYGSSVIRRCDNRMFFARQGSESC 60
           MENHAGKRRLDMIAAHLAPAGDISAAHAHVLPANCSSYGSSVIRRCDNRMFFARQGSESC
Sbjct: 1   MENHAGKRRLDMIAAHLAPAGDISAAHAHVLPANCSSYGSSVIRRCDNRMFFARQGSESC 60

Query: 61  GAYMRQASITEGNQGSNSQASLDAYEGPLYSRPATEGRIPGVGIEQPVAQNFNLIASEPP 120
           GAYMRQASITEGNQ SNSQASLDAYEGPLYSRPATEGRIPGVGIEQPVAQNFNLIASEPP
Sbjct: 61  GAYMRQASITEGNQRSNSQASLDAYEGPLYSRPATEGRIPGVGIEQPVAQNFNLIASEPP 120

Query: 121 LFARACFGISEHNNHFVSETKQPSSGSNGIGGCSPRMDVAESGRGYVLTVEIPGVKINDI 180
           LFARACFGISEHNNHFVSETKQPSSGSNGIGGCSPRMDVAESGRGYVLTVEIPGVKINDI
Sbjct: 121 LFARACFGISEHNNHFVSETKQPSSGSNGIGGCSPRMDVAESGRGYVLTVEIPGVKINDI 180

Query: 181 RVEVDDQKLTIIGKRSNQYHEMVGYSRESIRSYHKREILQGPYQVVWPLPININKDGVSA 240
           RVEVDDQKLTIIGKRSNQYHEMVG SRESIRSYHKREILQGPYQVVWPLPININKDGVSA
Sbjct: 181 RVEVDDQKLTIIGKRSNQYHEMVGCSRESIRSYHKREILQGPYQVVWPLPININKDGVSA 240

Query: 241 EFW 244
           EFW
Sbjct: 241 EFW 243

BLAST of MS006381 vs. ExPASy TrEMBL
Match: A0A6J1D2U0 (uncharacterized protein LOC111016826 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016826 PE=3 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 3.2e-133
Identity = 241/247 (97.57%), Postives = 241/247 (97.57%), Query Frame = 0

Query: 1   MENHAGKRRLDMIAAHLAPAGDISAAHAHVLPANCSSYGSSVIRRCDNRMFFARQGSESC 60
           MENHAGKRRLDMIAAHLAPAGDISAAHAHVLPANCSSYGSSVIRRCDNRMFFARQGSESC
Sbjct: 1   MENHAGKRRLDMIAAHLAPAGDISAAHAHVLPANCSSYGSSVIRRCDNRMFFARQGSESC 60

Query: 61  GAYMRQASITE----GNQGSNSQASLDAYEGPLYSRPATEGRIPGVGIEQPVAQNFNLIA 120
           GAYMRQASITE    GNQ SNSQASLDAYEGPLYSRPATEGRIPGVGIEQPVAQNFNLIA
Sbjct: 61  GAYMRQASITENIYQGNQRSNSQASLDAYEGPLYSRPATEGRIPGVGIEQPVAQNFNLIA 120

Query: 121 SEPPLFARACFGISEHNNHFVSETKQPSSGSNGIGGCSPRMDVAESGRGYVLTVEIPGVK 180
           SEPPLFARACFGISEHNNHFVSETKQPSSGSNGIGGCSPRMDVAESGRGYVLTVEIPGVK
Sbjct: 121 SEPPLFARACFGISEHNNHFVSETKQPSSGSNGIGGCSPRMDVAESGRGYVLTVEIPGVK 180

Query: 181 INDIRVEVDDQKLTIIGKRSNQYHEMVGYSRESIRSYHKREILQGPYQVVWPLPININKD 240
           INDIRVEVDDQKLTIIGKRSNQYHEMVG SRESIRSYHKREILQGPYQVVWPLPININKD
Sbjct: 181 INDIRVEVDDQKLTIIGKRSNQYHEMVGCSRESIRSYHKREILQGPYQVVWPLPININKD 240

Query: 241 GVSAEFW 244
           GVSAEFW
Sbjct: 241 GVSAEFW 247

BLAST of MS006381 vs. ExPASy TrEMBL
Match: H6TB35 (HSP27.8 OS=Citrullus lanatus OX=3654 PE=2 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 1.6e-116
Identity = 210/243 (86.42%), Postives = 222/243 (91.36%), Query Frame = 0

Query: 1   MENHAGKRRLDMIAAHLAPAGDISAAHAHVLPANCSSYGSSVIRRCDNRMFFARQGSESC 60
           ME+HAGKRRLD++ AHLAPAGDIS A+ HVLPANC SY SSVIRRCDN+ +FARQGSE+C
Sbjct: 1   MEHHAGKRRLDVLTAHLAPAGDISPANGHVLPANCCSYESSVIRRCDNKTYFARQGSEAC 60

Query: 61  GAYMRQASITEGNQGSNSQASLDAYEGPLYSRPATEGRIPGVGIEQPVAQNFNLIASEPP 120
           GAYMRQAS TEGNQ SNSQA  D  EGPLYSRPA EG+IP VGIEQPVAQNFNLIASEPP
Sbjct: 61  GAYMRQASTTEGNQRSNSQAFRDVSEGPLYSRPAKEGKIPSVGIEQPVAQNFNLIASEPP 120

Query: 121 LFARACFGISEHNNHFVSETKQPSSGSNGIGGCSPRMDVAESGRGYVLTVEIPGVKINDI 180
           LFARAC GI+E NNHF+SETKQPSSGSNGIGG SPRMDVAESGRGYVLTVEIPGVKINDI
Sbjct: 121 LFARACLGINEQNNHFISETKQPSSGSNGIGGLSPRMDVAESGRGYVLTVEIPGVKINDI 180

Query: 181 RVEVDDQKLTIIGKRSNQYHEMVGYSRESIRSYHKREILQGPYQVVWPLPININKDGVSA 240
           RVEVDDQKLTIIGKRSNQY E+VGYS +SI SYHKREILQGPYQVVWPLPININKDGV A
Sbjct: 181 RVEVDDQKLTIIGKRSNQYCEVVGYSSDSISSYHKREILQGPYQVVWPLPININKDGVFA 240

Query: 241 EFW 244
           EFW
Sbjct: 241 EFW 243

BLAST of MS006381 vs. ExPASy TrEMBL
Match: A0A6J1GNM5 (uncharacterized protein LOC111455581 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111455581 PE=3 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 1.2e-108
Identity = 199/245 (81.22%), Postives = 214/245 (87.35%), Query Frame = 0

Query: 1   MENHAGKRRLDMIAAHLAPAGDISAAHAHVLPANCSSYGSSVIRRCDNRMFFARQGSESC 60
           ME+HAGKRRLD++AAHLAPAGDIS    HVLPANC SYGSSVIRR DN+ +FARQGSE+C
Sbjct: 1   MEHHAGKRRLDVLAAHLAPAGDISTGQGHVLPANCCSYGSSVIRRFDNKTYFARQGSEAC 60

Query: 61  GAYMRQASITE--GNQGSNSQASLDAYEGPLYSRPATEGRIPGVGIEQPVAQNFNLIASE 120
           GAYMRQAS TE  GNQ SNSQ  LD  EGP+YSRPA +G IP +GIEQ + QNFNLIASE
Sbjct: 61  GAYMRQASATEVQGNQRSNSQPFLDVSEGPVYSRPAKDGGIPSIGIEQLMTQNFNLIASE 120

Query: 121 PPLFARACFGISEHNNHFVSETKQPSSGSNGIGGCSPRMDVAESGRGYVLTVEIPGVKIN 180
           PPLFARAC GI+E NNH +SE KQPSSGS G+GG SPRMDVAESGRGYVLTVEIPGVKIN
Sbjct: 121 PPLFARACLGINEQNNHVISEAKQPSSGSKGVGGLSPRMDVAESGRGYVLTVEIPGVKIN 180

Query: 181 DIRVEVDDQKLTIIGKRSNQYHEMVGYSRESIRSYHKREILQGPYQVVWPLPININKDGV 240
           DIRVEVDDQKLTIIGKRSNQY E+VGYS +SI SYHKREI QGPYQVVWPLPININKDGV
Sbjct: 181 DIRVEVDDQKLTIIGKRSNQYCEVVGYSSDSISSYHKREISQGPYQVVWPLPININKDGV 240

Query: 241 SAEFW 244
            AEFW
Sbjct: 241 LAEFW 245

BLAST of MS006381 vs. ExPASy TrEMBL
Match: A0A6J1GNK5 (uncharacterized protein LOC111455581 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111455581 PE=3 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 3.6e-108
Identity = 199/249 (79.92%), Postives = 214/249 (85.94%), Query Frame = 0

Query: 1   MENHAGKRRLDMIAAHLAPAGDISAAHAHVLPANCSSYGSSVIRRCDNRMFFARQGSESC 60
           ME+HAGKRRLD++AAHLAPAGDIS    HVLPANC SYGSSVIRR DN+ +FARQGSE+C
Sbjct: 1   MEHHAGKRRLDVLAAHLAPAGDISTGQGHVLPANCCSYGSSVIRRFDNKTYFARQGSEAC 60

Query: 61  GAYMRQASITE------GNQGSNSQASLDAYEGPLYSRPATEGRIPGVGIEQPVAQNFNL 120
           GAYMRQAS TE      GNQ SNSQ  LD  EGP+YSRPA +G IP +GIEQ + QNFNL
Sbjct: 61  GAYMRQASATEMIFQVQGNQRSNSQPFLDVSEGPVYSRPAKDGGIPSIGIEQLMTQNFNL 120

Query: 121 IASEPPLFARACFGISEHNNHFVSETKQPSSGSNGIGGCSPRMDVAESGRGYVLTVEIPG 180
           IASEPPLFARAC GI+E NNH +SE KQPSSGS G+GG SPRMDVAESGRGYVLTVEIPG
Sbjct: 121 IASEPPLFARACLGINEQNNHVISEAKQPSSGSKGVGGLSPRMDVAESGRGYVLTVEIPG 180

Query: 181 VKINDIRVEVDDQKLTIIGKRSNQYHEMVGYSRESIRSYHKREILQGPYQVVWPLPININ 240
           VKINDIRVEVDDQKLTIIGKRSNQY E+VGYS +SI SYHKREI QGPYQVVWPLPININ
Sbjct: 181 VKINDIRVEVDDQKLTIIGKRSNQYCEVVGYSSDSISSYHKREISQGPYQVVWPLPININ 240

Query: 241 KDGVSAEFW 244
           KDGV AEFW
Sbjct: 241 KDGVLAEFW 249

BLAST of MS006381 vs. TAIR 10
Match: AT1G06460.1 (alpha-crystallin domain 32.1 )

HSP 1 Score: 138.3 bits (347), Expect = 8.4e-33
Identity = 100/272 (36.76%), Postives = 140/272 (51.47%), Query Frame = 0

Query: 2   ENHAGKRRLDMIAAHLAPAGDISAAHAHVLPANCSSYGSSVIRRCDNRMFFARQGSESCG 61
           E+   +RRL   AAH       SA+ A ++P NCSS  ++VI+RCDN++ FARQ S   G
Sbjct: 4   ESITARRRLAAFAAHFPATSYDSASTASLVPLNCSSSLNTVIQRCDNKISFARQASSEQG 63

Query: 62  AYMRQASITE--GNQGSN-SQASLDAYEGPLY-SRPATEGRIPGVGIEQPVAQNFNL--- 121
            +MR AS  +   N G N     +   +  LY +R A+  +  G  + Q       +   
Sbjct: 64  FFMRPASPDDVLENLGMNLKNTVVRRGDNRLYFARQASSAQ--GFFMRQASTNERTIPHD 123

Query: 122 IASEPPLFARACFGISEHN-----NHFVSETKQ----PSSGSN--------------GIG 181
            A+     A    G    +      HF    K+    PS   N               +G
Sbjct: 124 AAASTKFSATKTTGFDSSSPAYAAPHFSKPAKEDIFFPSLSPNLQKERPKLDLPKLANLG 183

Query: 182 GC-SPRMDVAESGRGYVLTVEIPGVKINDIRVEVDDQKLTIIGKRSNQYHEMVGYSRESI 241
              SPR +VAES   YV+ +E+PG  INDIRVEVD+  LT+ G+R++   ++   ++ SI
Sbjct: 184 TVWSPRSNVAESTHSYVVAIELPGASINDIRVEVDNTNLTVTGRRTSICQKVDAGTKASI 243

Query: 242 RSYHKREILQGPYQVVWPLPININKDGVSAEF 243
             YHK+EILQGP++V WPLP N+NKD VSAEF
Sbjct: 244 LGYHKQEILQGPFKVSWPLPSNVNKDNVSAEF 273

BLAST of MS006381 vs. TAIR 10
Match: AT1G59860.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 46.6 bits (109), Expect = 3.3e-05
Identity = 31/103 (30.10%), Postives = 52/103 (50.49%), Query Frame = 0

Query: 139 ETKQPSSGSNGIGGCSPRMDVAESGRGYVLTVEIPGVKINDIRVEV-DDQKLTIIGKRSN 198
           E + PSS S+ I   + R+D  E+   +V   ++PG+K  +++VE+ DD  L I G+R  
Sbjct: 32  ELQFPSSSSSAI--ANARVDWKETAEAHVFKADLPGMKKEEVKVEIEDDSVLKISGERHV 91

Query: 199 QYHEMVGYSRESIRSYHKREILQGPYQVVWPLPININKDGVSA 241
           +         E   ++H+ E   G +   + LP N+  D V A
Sbjct: 92  E-------KEEKQDTWHRVERSSGGFSRKFRLPENVKMDQVKA 125

BLAST of MS006381 vs. TAIR 10
Match: AT5G12020.1 (17.6 kDa class II heat shock protein )

HSP 1 Score: 42.7 bits (99), Expect = 4.8e-04
Identity = 26/84 (30.95%), Postives = 42/84 (50.00%), Query Frame = 0

Query: 158 DVAESGRGYVLTVEIPGVKINDIRVEVD-DQKLTIIGKRSNQYHEMVGYSRESIRSYHKR 217
           DV E    Y   V++PG+K ++I+V+V+ D  L + G+R  +  E  G        Y + 
Sbjct: 48  DVIEHPNAYAFVVDMPGIKGDEIKVQVENDNVLVVSGERQRENKENEGV------KYVRM 107

Query: 218 EILQGPYQVVWPLPININKDGVSA 241
           E   G +   + LP N + D +SA
Sbjct: 108 ERRMGKFMRKFQLPENADLDKISA 125

BLAST of MS006381 vs. TAIR 10
Match: AT1G07400.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 42.0 bits (97), Expect = 8.2e-04
Identity = 30/104 (28.85%), Postives = 50/104 (48.08%), Query Frame = 0

Query: 139 ETKQPSSGSNGIGG-CSPRMDVAESGRGYVLTVEIPGVKINDIRVEV-DDQKLTIIGKRS 198
           E + PSS S       + R+D  E+   +V   ++PG+K  +++VE+ DD  L I G+R 
Sbjct: 31  ELQFPSSLSGETSAITNARVDWKETAEAHVFKADLPGMKKEEVKVEIEDDSVLKISGERH 90

Query: 199 NQYHEMVGYSRESIRSYHKREILQGPYQVVWPLPININKDGVSA 241
            +         E   ++H+ E   G +   + LP N+  D V A
Sbjct: 91  VE-------KEEKQDTWHRVERSSGQFSRKFKLPENVKMDQVKA 127

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022148040.11.2e-13499.18uncharacterized protein LOC111016826 isoform X2 [Momordica charantia][more]
XP_022148039.16.7e-13397.57uncharacterized protein LOC111016826 isoform X1 [Momordica charantia][more]
ADU55782.13.3e-11686.42HSP27.8 [Citrullus lanatus][more]
XP_038886846.14.1e-11485.60uncharacterized protein LOC120077066 [Benincasa hispida][more]
XP_022953065.12.6e-10881.22uncharacterized protein LOC111455581 isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q4UKR81.1e-1038.64Small heat shock protein C2 OS=Rickettsia felis (strain ATCC VR-1525 / URRWXCal2... [more]
Q4UJB11.0e-0631.25Small heat shock protein C4 OS=Rickettsia felis (strain ATCC VR-1525 / URRWXCal2... [more]
Q9XIE34.7e-0430.1017.6 kDa class I heat shock protein 1 OS=Arabidopsis thaliana OX=3702 GN=HSP17.6... [more]
P465164.7e-0431.3317.9 kDa class II heat shock protein OS=Helianthus annuus OX=4232 GN=HSP17.9 PE=... [more]
Q015444.7e-0434.5217.2 kDa class II heat shock protein OS=Ipomoea nil OX=35883 GN=SHSP-1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1D1T85.9e-13599.18uncharacterized protein LOC111016826 isoform X2 OS=Momordica charantia OX=3673 G... [more]
A0A6J1D2U03.2e-13397.57uncharacterized protein LOC111016826 isoform X1 OS=Momordica charantia OX=3673 G... [more]
H6TB351.6e-11686.42HSP27.8 OS=Citrullus lanatus OX=3654 PE=2 SV=1[more]
A0A6J1GNM51.2e-10881.22uncharacterized protein LOC111455581 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1GNK53.6e-10879.92uncharacterized protein LOC111455581 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT1G06460.18.4e-3336.76alpha-crystallin domain 32.1 [more]
AT1G59860.13.3e-0530.10HSP20-like chaperones superfamily protein [more]
AT5G12020.14.8e-0430.9517.6 kDa class II heat shock protein [more]
AT1G07400.18.2e-0428.85HSP20-like chaperones superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002068Alpha crystallin/Hsp20 domainPFAMPF00011HSP20coord: 159..241
e-value: 6.3E-8
score: 32.7
IPR002068Alpha crystallin/Hsp20 domainPROSITEPS01031SHSPcoord: 148..243
score: 14.765091
IPR008978HSP20-like chaperoneGENE3D2.60.40.790coord: 128..243
e-value: 1.7E-17
score: 65.4
IPR008978HSP20-like chaperoneSUPERFAMILY49764HSP20-like chaperonescoord: 147..242
NoneNo IPR availablePANTHERPTHR43670:SF61ALPHA-CRYSTALLIN DOMAIN 32.1coord: 6..243
NoneNo IPR availableCDDcd06464ACD_sHsps-likecoord: 158..242
e-value: 4.57031E-18
score: 74.5128
IPR045045Small heat shock protein RTM2-likePANTHERPTHR43670HEAT SHOCK PROTEIN 26coord: 6..243

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS006381.1MS006381.1mRNA