MS006372 (gene) Bitter gourd (TR) v1

Overview


Name	MS006372
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	GDSL esterase/lipase
Location	scaffold327: 179954 .. 182828 (-)
RNA-Seq Expression	MS006372
Synteny	MS006372

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTCCGGCCATTATTGTGTTTGGAGACTCCTCTGTGGATTCCGGCAATAACAATGTGGTTAAGACGGTGTTGAAAAGCAATTTCAGGCCTTATGGCCGGGACTTCGTCGGTGGTCAGGCCACCGGAAGGTTCTCCAACGGCCGCGTTCCGCCGGACTTTATTTCTGAAGCTTTTGGTCTAAAATCAACCATACCGGCTTACTTGGACCCCGGTTATACCATTGCCGACTTCGCCACCGGAGTTTGCTTCGCCTCTGCCGGAACTGGCTTCGACAACTCCACTTCCGATGTTTTAGTAAGTCTGTTTAATTTCTAAACTTTCAAGCTAATAAACTTGAAATTTTGTTGATATTTAAAATATGAAAATACTTAAACTTTTGAATTTTGATATCTAAAAGAGAAAAATACTAAAATTAATATTTTTTATAAAATTAATATCAATTTTTTAACGGTGATAAGGACAATATTTAATCCAAGAGTCAAGTATTTTCCTTTAAAATATATTATTGCTTGGAGAAGAATTTGTGGTTTTGGACAGAGTTCAAGTATTTGATAGTGCAAGTGTTAAAATGATAGAGATAGTGTGAACTATTAGATGCCTATTAGATATAATATTTAAATTAATATCTTTGTTAAGTTAAATTTTAAGAGTATATTTAATAGGTCGAACGTTTAATAGATCTTTGAACTCTTAATATCAATCTTTGATCAAAATTTCTCATTAATTTAACAGTTTTTTAAATTTTCAATTTTATGTCTAATAATTAGCTTCTTGACTTATTAAATACCTTTAAAATATATAAATCTATTAAACACAAAATTCAAAAATCTCATGATTGTGCATCTTTTGAGCTCACCCATTTTGACTTGATCTCATATTGATGTTCGTTTGAGCTTTTGGGCTTTGACATATATCAATATTTATGGGTTCCATCTCAATCATCTTTTAATACGTGATCGAGAACTCAATAATGTCGATGTATAATGAGGATTCTGTTGTTTATGAAACCGGGCAGAATGTGATACCTCTATGGAGGGAAGTGGAGTTTTTCAAGGACTATCAAAACAAGCTAAGAGCCTATATGGGCAATGAGAAAGCAAATGAAGTGATAAGAGAAGCTCTATATTTGGTAAGTTTAGGAACAAACGACTTCTTGGAAAATTACTACACAATTCCTCGGCGGAGACTCCAATTTACGATCCAACAGTACGAGGATTTCCTGCTCGAACTGGCCGGGAACTTCATCGGAGAACTTTACGGGCTCGGCGCGAGAAAAATATCGTTTACCGGGCTTCCTCCGATGGGGTGTTTGCCGCTGGAGAGAGCAACCAATGTCATGGGAAATTTTGGGTGCGTAGAGAAATACAACGCTGTGGCTTTGGAATTCAATAGGAAGTTGGAGGGATTCGTGGGGGCATTGAATAGGCAGCTCCCAGGCCTTACAATGCTCTTTGCAAATCCTTTTCCCATTTTTTATCAGATCATCAGCCAGCCTTACTTATACGGTATGAAATTAAACATCTTATCTTTCAGCTTTGTGCTTCTTTTTCATTTTTGTTCGTGATTTCTTTTAAATAAATCATTTATGGTTCTATATCAAAATAAATTGAGAACGAGAAGAAGTAACACCATGTAGTTTACAAACAAGATCAGAGATGTTCGTTTAAGTTTGTTGGACTCTAAATATATCTTATCAATATTCGTTTAGTGACATATCCTCTCAATAAACCAAGGCTTAGGAACATTGTTAAATTACGTCCCTTATCTTTTTAATGTGGGATATTTAAAAAAAAAAAAAAAAAAAAGATGAGGTTTCACCCACTTTTAGAATAGTAGACCCCATTTCCCCTATTTTTGAGTTCTAGATAATAATTTTGTTAAGGATCACACATAATAAGAGGCCTAACCGGTTTATAATATAAGTGAGATACTTCTCTAATTGTCAATTAGTTTTGAGTTGGAACTCATGAATATTAATATGATATCATAACCCAAAAGTTCGAACTCGAAAAAATGAGATCTGGGCCAAGATTGGTGGACCCAAAAGAGGCAACTATCTTAAGGAGCATGTTAAGGATCGGAAGATAAAGTACCTAATAAGGATTTATAATATAAGTGGGCTACTCCGTCGAAAATATTTAGATGTTGACGACTAATGAGGATAGTACAAAAAATATGTGAAAGTTGGTTGGGTAGGAGAACCAAACCACCATTTATAGAAGACAACACAAACGGTAGGTGTCTAGGGCTTTGGTTGTCTGCATTTTCTGTGCGTAGTTAGCTCATTGAAGGTTTGGTCATACTCATTAATATATATCTCTACGCCATCTTTAAGACACTGCCAACCCTCCCCATCTTTCTCTCTCTACATTTTTTACTCTTTTTAGAGTAAAAGTTCTTAAAAGTTCTGGATTGTTTCTTAAAAAGGTTTTGATTTAGTTTCTTACCAGGTGAATATTAAAATTGACCCAGGAATTTTTTGGGTAACATAATACTCGGTGAATTGGCAAAATTTTGAACCAGAAATTTGGGGCACTAAAAGATAAAATCTTTGGGGTAGAAATTTCAATATTTCATCTCTTGAGGGATTAATATTAAAATATAAAGGACTTGATTAAAAAAATATTTGAATATTAAGGACTAAAACATATATTTAACTAATATCGGACACGATAAATGGGCAGGATTTGAAGTGGCTGGAAAGGCATGCTGTGCAACTGGGACATTTGAGATGAGCTATCTGTGCAACCAAGAAAACCCATTTACTTGCATGGACGCAAACAAATATGTGTTTTGGGATGCCTTTCATCCCACTCAGAAGACCAACCAAATTATTGTCAATGATTTGCTTAGAATTCTTCTTCCTATGTTTCGA

mRNA sequence

GTTCCGGCCATTATTGTGTTTGGAGACTCCTCTGTGGATTCCGGCAATAACAATGTGGTTAAGACGGTGTTGAAAAGCAATTTCAGGCCTTATGGCCGGGACTTCGTCGGTGGTCAGGCCACCGGAAGGTTCTCCAACGGCCGCGTTCCGCCGGACTTTATTTCTGAAGCTTTTGGTCTAAAATCAACCATACCGGCTTACTTGGACCCCGGTTATACCATTGCCGACTTCGCCACCGGAGTTTGCTTCGCCTCTGCCGGAACTGGCTTCGACAACTCCACTTCCGATGTTTTAAATGTGATACCTCTATGGAGGGAAGTGGAGTTTTTCAAGGACTATCAAAACAAGCTAAGAGCCTATATGGGCAATGAGAAAGCAAATGAAGTGATAAGAGAAGCTCTATATTTGGTAAGTTTAGGAACAAACGACTTCTTGGAAAATTACTACACAATTCCTCGGCGGAGACTCCAATTTACGATCCAACAGTACGAGGATTTCCTGCTCGAACTGGCCGGGAACTTCATCGGAGAACTTTACGGGCTCGGCGCGAGAAAAATATCGTTTACCGGGCTTCCTCCGATGGGGTGTTTGCCGCTGGAGAGAGCAACCAATGTCATGGGAAATTTTGGGTGCGTAGAGAAATACAACGCTGTGGCTTTGGAATTCAATAGGAAGTTGGAGGGATTCGTGGGGGCATTGAATAGGCAGCTCCCAGGCCTTACAATGCTCTTTGCAAATCCTTTTCCCATTTTTTATCAGATCATCAGCCAGCCTTACTTATACGGATTTGAAGTGGCTGGAAAGGCATGCTGTGCAACTGGGACATTTGAGATGAGCTATCTGTGCAACCAAGAAAACCCATTTACTTGCATGGACGCAAACAAATATGTGTTTTGGGATGCCTTTCATCCCACTCAGAAGACCAACCAAATTATTGTCAATGATTTGCTTAGAATTCTTCTTCCTATGTTTCGA

Coding sequence (CDS)

GTTCCGGCCATTATTGTGTTTGGAGACTCCTCTGTGGATTCCGGCAATAACAATGTGGTTAAGACGGTGTTGAAAAGCAATTTCAGGCCTTATGGCCGGGACTTCGTCGGTGGTCAGGCCACCGGAAGGTTCTCCAACGGCCGCGTTCCGCCGGACTTTATTTCTGAAGCTTTTGGTCTAAAATCAACCATACCGGCTTACTTGGACCCCGGTTATACCATTGCCGACTTCGCCACCGGAGTTTGCTTCGCCTCTGCCGGAACTGGCTTCGACAACTCCACTTCCGATGTTTTAAATGTGATACCTCTATGGAGGGAAGTGGAGTTTTTCAAGGACTATCAAAACAAGCTAAGAGCCTATATGGGCAATGAGAAAGCAAATGAAGTGATAAGAGAAGCTCTATATTTGGTAAGTTTAGGAACAAACGACTTCTTGGAAAATTACTACACAATTCCTCGGCGGAGACTCCAATTTACGATCCAACAGTACGAGGATTTCCTGCTCGAACTGGCCGGGAACTTCATCGGAGAACTTTACGGGCTCGGCGCGAGAAAAATATCGTTTACCGGGCTTCCTCCGATGGGGTGTTTGCCGCTGGAGAGAGCAACCAATGTCATGGGAAATTTTGGGTGCGTAGAGAAATACAACGCTGTGGCTTTGGAATTCAATAGGAAGTTGGAGGGATTCGTGGGGGCATTGAATAGGCAGCTCCCAGGCCTTACAATGCTCTTTGCAAATCCTTTTCCCATTTTTTATCAGATCATCAGCCAGCCTTACTTATACGGATTTGAAGTGGCTGGAAAGGCATGCTGTGCAACTGGGACATTTGAGATGAGCTATCTGTGCAACCAAGAAAACCCATTTACTTGCATGGACGCAAACAAATATGTGTTTTGGGATGCCTTTCATCCCACTCAGAAGACCAACCAAATTATTGTCAATGATTTGCTTAGAATTCTTCTTCCTATGTTTCGA

Protein sequence

VPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNGRVPPDFISEAFGLKSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNVIPLWREVEFFKDYQNKLRAYMGNEKANEVIREALYLVSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGNFIGELYGLGARKISFTGLPPMGCLPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNRQLPGLTMLFANPFPIFYQIISQPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWDAFHPTQKTNQIIVNDLLRILLPMFR

Homology

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BLAST of MS006372 vs. NCBI nr
Match: XP_022147832.1 (GDSL esterase/lipase At2g42990-like isoform X1 [Momordica charantia])

HSP 1 Score: 669.8 bits (1727), Expect = 1.1e-188
Identity = 323/325 (99.38%), Postives = 323/325 (99.38%), Query Frame = 0


Query: 1   VPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNGRVPPDFISEAFGL 60
           VPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNGRVPPDFISEAFGL
Sbjct: 27  VPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNGRVPPDFISEAFGL 86

Query: 61  KSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNVIPLWREVEFFKDYQNKLRAY 120
           KSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNVIPLWREVEFFKDYQNKLRAY
Sbjct: 87  KSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNVIPLWREVEFFKDYQNKLRAY 146

Query: 121 MGNEKANEVIREALYLVSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGNFIGELYG 180
           MGNEKANEVIREALYLVSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAG FIGELYG
Sbjct: 147 MGNEKANEVIREALYLVSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGKFIGELYG 206

Query: 181 LGARKISFTGLPPMGCLPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNRQLPGL 240
           LGARKISFTGLPPMGCLPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALN QLPGL
Sbjct: 207 LGARKISFTGLPPMGCLPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNMQLPGL 266

Query: 241 TMLFANPFPIFYQIISQPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWD 300
           TMLFANPFPIFYQIISQPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWD
Sbjct: 267 TMLFANPFPIFYQIISQPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWD 326

Query: 301 AFHPTQKTNQIIVNDLLRILLPMFR 326
           AFHPTQKTNQIIVNDLLRILLPMFR
Sbjct: 327 AFHPTQKTNQIIVNDLLRILLPMFR 351

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BLAST of MS006372 vs. NCBI nr
Match: XP_022930712.1 (GDSL esterase/lipase At2g42990-like [Cucurbita moschata])

HSP 1 Score: 592.4 bits (1526), Expect = 2.3e-165
Identity = 279/325 (85.85%), Postives = 300/325 (92.31%), Query Frame = 0


Query: 1   VPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNGRVPPDFISEAFGL 60
           VPAIIVFGDSSVDSGNNN +KT+LKSNF+PYGRDF GGQ TGRFSNGRVPPDFISEAFGL
Sbjct: 23  VPAIIVFGDSSVDSGNNNAIKTLLKSNFKPYGRDFYGGQPTGRFSNGRVPPDFISEAFGL 82

Query: 61  KSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNVIPLWREVEFFKDYQNKLRAY 120
           K TIPAYLDP +TIADFATGVCFASAGTGFDN+TSDVLNVIPLWREVE +K+YQ KLR Y
Sbjct: 83  KPTIPAYLDPAFTIADFATGVCFASAGTGFDNATSDVLNVIPLWREVELYKEYQAKLRGY 142

Query: 121 MGNEKANEVIREALYLVSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGNFIGELYG 180
           +GNEKANEVI+EALYLVSLGTNDFLENYYTIPRRRLQF++QQYEDFLL+LAG FIGE+Y 
Sbjct: 143 LGNEKANEVIKEALYLVSLGTNDFLENYYTIPRRRLQFSVQQYEDFLLQLAGKFIGEIYS 202

Query: 181 LGARKISFTGLPPMGCLPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNRQLPGL 240
           LG RKIS +GLPPMGCLPLERATNVM NF CVEK+N VALEFN KLEGFV ALNRQLPGL
Sbjct: 203 LGVRKISISGLPPMGCLPLERATNVMSNFECVEKFNLVALEFNAKLEGFVWALNRQLPGL 262

Query: 241 TMLFANPFPIFYQIISQPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWD 300
           TMLF+NP+ IFYQII+QPYLYGFEVAGKACC TGTFEMSYLC+QEN  TC DA KYVFWD
Sbjct: 263 TMLFSNPYAIFYQIITQPYLYGFEVAGKACCGTGTFEMSYLCSQENSLTCSDATKYVFWD 322

Query: 301 AFHPTQKTNQIIVNDLLRILLPMFR 326
           AFHPT+KTNQIIVNDLL+ LLP FR
Sbjct: 323 AFHPTEKTNQIIVNDLLQTLLPTFR 347

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BLAST of MS006372 vs. NCBI nr
Match: XP_023529877.1 (GDSL esterase/lipase At2g42990-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 592.0 bits (1525), Expect = 3.0e-165
Identity = 279/325 (85.85%), Postives = 300/325 (92.31%), Query Frame = 0


Query: 1   VPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNGRVPPDFISEAFGL 60
           VPAIIVFGDSSVDSGNNN +KT+LKSNF+PYGRDF GGQ TGRFSNGRVPPDFISEAFGL
Sbjct: 23  VPAIIVFGDSSVDSGNNNAIKTLLKSNFKPYGRDFYGGQPTGRFSNGRVPPDFISEAFGL 82

Query: 61  KSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNVIPLWREVEFFKDYQNKLRAY 120
           K TIPAYLDP +TIADFATGVCFASAGTGFDN+TSDVLNVIPLWREVE +K+YQ KLR Y
Sbjct: 83  KPTIPAYLDPAFTIADFATGVCFASAGTGFDNATSDVLNVIPLWREVELYKEYQAKLRGY 142

Query: 121 MGNEKANEVIREALYLVSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGNFIGELYG 180
           +GNEKANEVIREALYLVSLGTNDFLENYYTIPRRRLQF++QQYEDFLL+LAG FIGE+Y 
Sbjct: 143 LGNEKANEVIREALYLVSLGTNDFLENYYTIPRRRLQFSVQQYEDFLLQLAGKFIGEIYS 202

Query: 181 LGARKISFTGLPPMGCLPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNRQLPGL 240
           LG RKIS +GLPPMGCLPLERATNVM NF CVEK+N VALEFN KLEGFV ALNRQLPGL
Sbjct: 203 LGVRKISISGLPPMGCLPLERATNVMSNFECVEKFNLVALEFNAKLEGFVWALNRQLPGL 262

Query: 241 TMLFANPFPIFYQIISQPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWD 300
           TMLF+NP+ IFYQII++PYLYGFEVAGKACC TGTFEMSYLC+QEN  TC DA KYVFWD
Sbjct: 263 TMLFSNPYAIFYQIITKPYLYGFEVAGKACCGTGTFEMSYLCSQENSLTCSDATKYVFWD 322

Query: 301 AFHPTQKTNQIIVNDLLRILLPMFR 326
           AFHPT+KTNQIIVNDLL+ LLP FR
Sbjct: 323 AFHPTEKTNQIIVNDLLQTLLPTFR 347

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BLAST of MS006372 vs. NCBI nr
Match: KAG6589280.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 591.3 bits (1523), Expect = 5.2e-165
Identity = 278/325 (85.54%), Postives = 301/325 (92.62%), Query Frame = 0


Query: 1   VPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNGRVPPDFISEAFGL 60
           VPAIIVFGDSSVDSGNNN +KT+LKSNF+PYGRDF GGQ TGRFSNGRVPPDFISEAFGL
Sbjct: 23  VPAIIVFGDSSVDSGNNNAIKTLLKSNFKPYGRDFYGGQPTGRFSNGRVPPDFISEAFGL 82

Query: 61  KSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNVIPLWREVEFFKDYQNKLRAY 120
           K TIPAYLDP +TIADFATGVCFASAGTGFD++TSDVLNVIPLWREVE +K+YQ KLR Y
Sbjct: 83  KPTIPAYLDPAFTIADFATGVCFASAGTGFDDATSDVLNVIPLWREVELYKEYQAKLRGY 142

Query: 121 MGNEKANEVIREALYLVSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGNFIGELYG 180
           +GNEKANEVI+EALYLVSLGTNDFLENYYTIPRRRLQF++QQYEDFLL+LAG FIGE+Y 
Sbjct: 143 LGNEKANEVIKEALYLVSLGTNDFLENYYTIPRRRLQFSVQQYEDFLLQLAGKFIGEIYS 202

Query: 181 LGARKISFTGLPPMGCLPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNRQLPGL 240
           LG RKIS +GLPPMGCLPLERATNVM NF CVEK+N VALEFN KLEGFV ALNRQLPGL
Sbjct: 203 LGVRKISISGLPPMGCLPLERATNVMSNFECVEKFNLVALEFNAKLEGFVWALNRQLPGL 262

Query: 241 TMLFANPFPIFYQIISQPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWD 300
           TMLF+NP+ IFYQII++PYLYGFEVAGKACC TGTFEMSYLC+QEN  TC DA KYVFWD
Sbjct: 263 TMLFSNPYAIFYQIITKPYLYGFEVAGKACCGTGTFEMSYLCSQENSLTCSDATKYVFWD 322

Query: 301 AFHPTQKTNQIIVNDLLRILLPMFR 326
           AFHPT+KTNQIIVNDLL+ LLPMFR
Sbjct: 323 AFHPTEKTNQIIVNDLLQTLLPMFR 347

[Close]

BLAST of MS006372 vs. NCBI nr
Match: XP_022989111.1 (GDSL esterase/lipase At2g42990-like [Cucurbita maxima])

HSP 1 Score: 588.6 bits (1516), Expect = 3.4e-164
Identity = 278/325 (85.54%), Postives = 299/325 (92.00%), Query Frame = 0


Query: 1   VPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNGRVPPDFISEAFGL 60
           VPAIIVFGDSSVDSGNNN +KT+LKSNF PYGRDF GGQ TGRFSNGRVPPDFISEAFGL
Sbjct: 23  VPAIIVFGDSSVDSGNNNAIKTLLKSNFMPYGRDFYGGQPTGRFSNGRVPPDFISEAFGL 82

Query: 61  KSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNVIPLWREVEFFKDYQNKLRAY 120
           K TIPAYLDP +TIADFATGVCFASAGTGFDN+TSDVLNVIPLWREVE +K+YQ KLR Y
Sbjct: 83  KPTIPAYLDPAFTIADFATGVCFASAGTGFDNATSDVLNVIPLWREVELYKEYQAKLRGY 142

Query: 121 MGNEKANEVIREALYLVSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGNFIGELYG 180
           +GNEKANEVI+EALYLVSLGTNDFLENYYTIPRRRLQF++QQYEDFLL+LAG FI E+Y 
Sbjct: 143 VGNEKANEVIKEALYLVSLGTNDFLENYYTIPRRRLQFSVQQYEDFLLQLAGKFIREIYS 202

Query: 181 LGARKISFTGLPPMGCLPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNRQLPGL 240
           LG RKIS +GLPPMGCLPLERATNVM NF CVEK+N VALEFN KLEGFV ALNRQLPGL
Sbjct: 203 LGVRKISISGLPPMGCLPLERATNVMSNFECVEKFNLVALEFNAKLEGFVWALNRQLPGL 262

Query: 241 TMLFANPFPIFYQIISQPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWD 300
           TMLF+NP+ IFYQII++PYLYGFEVAGKACC TGTFEMSYLC+QEN  TC DA KYVFWD
Sbjct: 263 TMLFSNPYAIFYQIITKPYLYGFEVAGKACCGTGTFEMSYLCSQENSLTCSDATKYVFWD 322

Query: 301 AFHPTQKTNQIIVNDLLRILLPMFR 326
           AFHPT+KTNQIIVNDLL+ LLPMFR
Sbjct: 323 AFHPTEKTNQIIVNDLLQTLLPMFR 347

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BLAST of MS006372 vs. ExPASy Swiss-Prot
Match: Q67ZI9 (GDSL esterase/lipase At2g42990 OS=Arabidopsis thaliana OX=3702 GN=At2g42990 PE=2 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 6.9e-128
Identity = 203/324 (62.65%), Postives = 261/324 (80.56%), Query Frame = 0


Query: 1   VPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNGRVPPDFISEAFGL 60
           +PAIIVFGDSSVDSGNNN + T+ ++NF PYGRDF GG+ATGRF NGR+  DF SEA+GL
Sbjct: 26  IPAIIVFGDSSVDSGNNNFISTMARANFEPYGRDFPGGRATGRFCNGRLSSDFTSEAYGL 85

Query: 61  KSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNVIPLWREVEFFKDYQNKLRAY 120
           K T+PAYLDP Y I+DFATGVCFASAGTG+DNST+DVL VIPLW+EVE+FK+YQ+ L AY
Sbjct: 86  KPTVPAYLDPSYNISDFATGVCFASAGTGYDNSTADVLGVIPLWKEVEYFKEYQSNLSAY 145

Query: 121 MGNEKANEVIREALYLVSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGNFIGELYG 180
           +G+ +A ++IRE+LY+VS+GTNDFLENYYT+P RR QF+I QY+DFL+E+A  F+ ++Y 
Sbjct: 146 LGHRRAAKIIRESLYIVSIGTNDFLENYYTLPDRRSQFSISQYQDFLVEIAEVFLKDIYR 205

Query: 181 LGARKISFTGLPPMGCLPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNRQLPGL 240
           LGARK+SFTG+ PMGCLPLER TN+   F C   YN +A++FN +L   V  LNR+L G+
Sbjct: 206 LGARKMSFTGISPMGCLPLERVTNLDDPFSCARSYNDLAVDFNGRLRRLVTKLNRELTGI 265

Query: 241 TMLFANPFPIFYQIISQPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWD 300
            + FANP+ I + I+++P LYG E++  ACC TG FEM +LC Q+NP TC DANK+VFWD
Sbjct: 266 KIYFANPYDIMWDIVTKPNLYGLEISSSACCGTGLFEMGFLCGQDNPLTCSDANKFVFWD 325

Query: 301 AFHPTQKTNQIIVNDLLRILLPMF 325
           AFHPT++TNQI+ +   + L  +F
Sbjct: 326 AFHPTERTNQIVSDHFFKHLKNLF 349

[Close]

BLAST of MS006372 vs. ExPASy Swiss-Prot
Match: Q8VY93 (GDSL esterase/lipase At4g26790 OS=Arabidopsis thaliana OX=3702 GN=At4g26790 PE=2 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 1.9e-122
Identity = 196/324 (60.49%), Postives = 258/324 (79.63%), Query Frame = 0


Query: 2   PAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNGRVPPDFISEAFGLK 61
           PA+IVFGDS+VDSGNNN + TVLKSNF+PYGRD+  G+ATGRFSNGR+ PDFISE  GLK
Sbjct: 28  PALIVFGDSTVDSGNNNQISTVLKSNFQPYGRDYFDGKATGRFSNGRIAPDFISEGLGLK 87

Query: 62  STIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNVIPLWREVEFFKDYQNKLRAYM 121
           + +PAYLDP Y IADFATGVCFASAGTG DN+TS VL+V+PLW+EVE++K+YQ +LR+Y+
Sbjct: 88  NAVPAYLDPAYNIADFATGVCFASAGTGLDNATSAVLSVMPLWKEVEYYKEYQTRLRSYL 147

Query: 122 GNEKANEVIREALYLVSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGNFIGELYGL 181
           G EKANE+I E+LYL+S+GTNDFLENYY +PR+  ++++ +Y+ FL+ +A +F+ ++Y L
Sbjct: 148 GEEKANEIISESLYLISIGTNDFLENYYLLPRKLRKYSVNEYQYFLIGIAADFVTDIYRL 207

Query: 182 GARKISFTGLPPMGCLPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNRQLPGLT 241
           GARK+S +GL P GCLPLER T +     C+E+YN VA +FN K+E  V  LNR L G+ 
Sbjct: 208 GARKMSLSGLSPFGCLPLERTTQLFYGSKCIEEYNIVARDFNIKMEEKVFQLNRDLNGIQ 267

Query: 242 MLFANPFPIFYQIISQPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWDA 301
           ++F+NP+ +  +II  P  +GFE    ACC TG +EMSYLC++ NPFTC DA+KYVFWD+
Sbjct: 268 LVFSNPYDLVSEIIYHPEAFGFENVRSACCGTGYYEMSYLCDKMNPFTCSDASKYVFWDS 327

Query: 302 FHPTQKTNQIIVNDLLRILLPMFR 326
           FHPT+KTN I+ N +L+  L  F+
Sbjct: 328 FHPTEKTNAIVANHVLKYDLSRFQ 351

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BLAST of MS006372 vs. ExPASy Swiss-Prot
Match: Q9SJB4 (GDSL esterase/lipase At2g04570 OS=Arabidopsis thaliana OX=3702 GN=At2g04570 PE=2 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 6.2e-121
Identity = 194/324 (59.88%), Postives = 250/324 (77.16%), Query Frame = 0


Query: 1   VPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNGRVPPDFISEAFGL 60
           +PAIIVFGDSSVD+GNNN + TV +SNF PYGRDFVGG+ TGRF NG++  DF+SEA GL
Sbjct: 26  IPAIIVFGDSSVDAGNNNYIPTVARSNFEPYGRDFVGGKPTGRFCNGKIATDFMSEALGL 85

Query: 61  KSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNVIPLWREVEFFKDYQNKLRAY 120
           K  IPAYLDP Y I+DFATGV FASA TG+DN+TSDVL+V+PLW+++E++K+YQ KL+AY
Sbjct: 86  KPIIPAYLDPSYNISDFATGVTFASAATGYDNATSDVLSVLPLWKQLEYYKEYQTKLKAY 145

Query: 121 MGNEKANEVIREALYLVSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGNFIGELYG 180
            G ++  E I  +LYL+S+GTNDFLENY+  P R  Q+++  Y+DFL  +A  F+ +L+G
Sbjct: 146 QGKDRGTETIESSLYLISIGTNDFLENYFAFPGRSSQYSVSLYQDFLAGIAKEFVKKLHG 205

Query: 181 LGARKISFTGLPPMGCLPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNRQLPGL 240
           LGARKIS  GLPPMGC+PLERATN+     CV +YN +A++FN KL+  V  L+++LPG 
Sbjct: 206 LGARKISLGGLPPMGCMPLERATNIGTGGECVGRYNDIAVQFNSKLDKMVEKLSKELPGS 265

Query: 241 TMLFANPFPIFYQIISQPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWD 300
            ++F+NP+  F +II  P  +GFEV G ACCATG FEM Y C + NPFTC +A+KYVFWD
Sbjct: 266 NLVFSNPYEPFMRIIKNPSSFGFEVVGAACCATGMFEMGYGCQRNNPFTCTNADKYVFWD 325

Query: 301 AFHPTQKTNQIIVNDLLRILLPMF 325
           +FHPTQKTN I+ N L+    P F
Sbjct: 326 SFHPTQKTNHIMANALMNSTFPHF 349

[Close]

BLAST of MS006372 vs. ExPASy Swiss-Prot
Match: Q9FHW9 (GDSL esterase/lipase At5g42170 OS=Arabidopsis thaliana OX=3702 GN=At5g42170/At5g42160 PE=3 SV=2)

HSP 1 Score: 292.4 bits (747), Expect = 6.5e-78
Identity = 140/317 (44.16%), Postives = 198/317 (62.46%), Query Frame = 0


Query: 1   VPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNGRVPPDFISEAFGL 60
           +P II FGDS VDSGNNN ++T LK NF PYG+DF G  ATGRFS+GRVP D ++E  G+
Sbjct: 48  IPGIITFGDSIVDSGNNNHLRTALKCNFPPYGKDFPGKIATGRFSDGRVPSDIVAERLGI 107

Query: 61  KSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNVIPLWREVEFFKDYQNKLRAY 120
             TIPAYL+P     D   GV FAS G+G+D  T+ ++ V+ L  +++ F++Y+NKL+  
Sbjct: 108 AETIPAYLNPKLKNEDLLKGVNFASGGSGYDPLTAKLVKVVSLSDQLKNFQEYKNKLKVI 167

Query: 121 MGNEKANEVIREALYLVSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGNFIGELYG 180
           +G EKAN +++ +LYLV   +ND    Y     R +++    Y D+L + A  F+  LYG
Sbjct: 168 VGEEKANFLVKNSLYLVVASSNDIAHTY---TARSIKYNKTSYADYLADSASKFVSALYG 227

Query: 181 LGARKISFTGLPPMGCLPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNRQLPGL 240
           LGAR+I      P+GC+P  R         C EK N VA  FN K+   + AL ++LP  
Sbjct: 228 LGARRIGVFSAVPVGCVPAARTLRGKLKRRCSEKLNEVARNFNAKISPTLEALGKELPDS 287

Query: 241 TMLFANPFPIFYQIISQPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWD 300
            ++  +       +I  P  YGFEV+ + CC TG  E+ +LCN+ NPFTC +++ Y+FWD
Sbjct: 288 RVVLIDVCDTLNDMIENPKNYGFEVSNRGCCGTGLVEVLFLCNKINPFTCKNSSSYIFWD 347

Query: 301 AFHPTQKTNQIIVNDLL 318
           ++HPT+K  QIIV+ LL
Sbjct: 348 SYHPTEKAYQIIVDKLL 361

[Close]

BLAST of MS006372 vs. ExPASy Swiss-Prot
Match: Q9C653 (GDSL esterase/lipase At1g58525 OS=Arabidopsis thaliana OX=3702 GN=At1g58525 PE=3 SV=2)

HSP 1 Score: 291.6 bits (745), Expect = 1.1e-77
Identity = 138/320 (43.12%), Postives = 208/320 (65.00%), Query Frame = 0


Query: 1   VPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNGRVPPDFISEAFGL 60
           +PA+IVFGDS +D+GNNN + T+LK NF PYG+D+ GG ATGRFS+GRVP D I+E  GL
Sbjct: 28  IPALIVFGDSIMDTGNNNNLPTLLKCNFPPYGKDYPGGDATGRFSDGRVPSDLIAEKLGL 87

Query: 61  KSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNVIPLWREVEFFKDYQNKLRAY 120
             T+PAY++      D   GV FAS GTG+D  T+ +++VI +W ++ +FK+Y +K++ +
Sbjct: 88  AKTLPAYMNSYLKPEDLLKGVTFASRGTGYDPLTAKIMSVISVWDQLIYFKEYISKIKRH 147

Query: 121 MGNEKANEVIREALYLVSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGNFIGELYG 180
            G EKA +++  + +LV   +ND    Y     R   +    Y +FL + A +F+ EL+ 
Sbjct: 148 FGEEKAKDILEHSFFLVVSSSNDLAHTYLAQAHR---YDRTSYANFLADSAVHFVRELHK 207

Query: 181 LGARKISFTGLPPMGCLPLERATNVMGNF---GCVEKYNAVALEFNRKLEGFVGALNRQL 240
           LGARKI      P+GC+PL+R   V G F   GC E  N +A +FN +L   + +L+++L
Sbjct: 208 LGARKIGVFSAVPVGCVPLQR--TVFGGFFTRGCNEPLNNMAKQFNARLSPALDSLDKEL 267

Query: 241 PGLTMLFANPFPIFYQIISQPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYV 300
            G+ +L+ N +   + +I  P  YGF+VA K CC  G   +SYLCN  NPFTC +++ Y+
Sbjct: 268 DGV-ILYINVYDTLFDMIQHPKKYGFDVADKGCCGKGLLTISYLCNSLNPFTCSNSSSYI 327

Query: 301 FWDAFHPTQKTNQIIVNDLL 318
           FWD++HP+++  Q+IV++LL
Sbjct: 328 FWDSYHPSERAYQVIVDNLL 341

[Close]

BLAST of MS006372 vs. ExPASy TrEMBL
Match: A0A6J1D3F6 (GDSL esterase/lipase At2g42990-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016676 PE=3 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 5.5e-189
Identity = 323/325 (99.38%), Postives = 323/325 (99.38%), Query Frame = 0


Query: 1   VPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNGRVPPDFISEAFGL 60
           VPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNGRVPPDFISEAFGL
Sbjct: 27  VPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNGRVPPDFISEAFGL 86

Query: 61  KSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNVIPLWREVEFFKDYQNKLRAY 120
           KSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNVIPLWREVEFFKDYQNKLRAY
Sbjct: 87  KSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNVIPLWREVEFFKDYQNKLRAY 146

Query: 121 MGNEKANEVIREALYLVSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGNFIGELYG 180
           MGNEKANEVIREALYLVSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAG FIGELYG
Sbjct: 147 MGNEKANEVIREALYLVSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGKFIGELYG 206

Query: 181 LGARKISFTGLPPMGCLPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNRQLPGL 240
           LGARKISFTGLPPMGCLPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALN QLPGL
Sbjct: 207 LGARKISFTGLPPMGCLPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNMQLPGL 266

Query: 241 TMLFANPFPIFYQIISQPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWD 300
           TMLFANPFPIFYQIISQPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWD
Sbjct: 267 TMLFANPFPIFYQIISQPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWD 326

Query: 301 AFHPTQKTNQIIVNDLLRILLPMFR 326
           AFHPTQKTNQIIVNDLLRILLPMFR
Sbjct: 327 AFHPTQKTNQIIVNDLLRILLPMFR 351

[Close]

BLAST of MS006372 vs. ExPASy TrEMBL
Match: A0A6J1ERD0 (GDSL esterase/lipase At2g42990-like OS=Cucurbita moschata OX=3662 GN=LOC111437110 PE=3 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 1.1e-165
Identity = 279/325 (85.85%), Postives = 300/325 (92.31%), Query Frame = 0


Query: 1   VPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNGRVPPDFISEAFGL 60
           VPAIIVFGDSSVDSGNNN +KT+LKSNF+PYGRDF GGQ TGRFSNGRVPPDFISEAFGL
Sbjct: 23  VPAIIVFGDSSVDSGNNNAIKTLLKSNFKPYGRDFYGGQPTGRFSNGRVPPDFISEAFGL 82

Query: 61  KSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNVIPLWREVEFFKDYQNKLRAY 120
           K TIPAYLDP +TIADFATGVCFASAGTGFDN+TSDVLNVIPLWREVE +K+YQ KLR Y
Sbjct: 83  KPTIPAYLDPAFTIADFATGVCFASAGTGFDNATSDVLNVIPLWREVELYKEYQAKLRGY 142

Query: 121 MGNEKANEVIREALYLVSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGNFIGELYG 180
           +GNEKANEVI+EALYLVSLGTNDFLENYYTIPRRRLQF++QQYEDFLL+LAG FIGE+Y 
Sbjct: 143 LGNEKANEVIKEALYLVSLGTNDFLENYYTIPRRRLQFSVQQYEDFLLQLAGKFIGEIYS 202

Query: 181 LGARKISFTGLPPMGCLPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNRQLPGL 240
           LG RKIS +GLPPMGCLPLERATNVM NF CVEK+N VALEFN KLEGFV ALNRQLPGL
Sbjct: 203 LGVRKISISGLPPMGCLPLERATNVMSNFECVEKFNLVALEFNAKLEGFVWALNRQLPGL 262

Query: 241 TMLFANPFPIFYQIISQPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWD 300
           TMLF+NP+ IFYQII+QPYLYGFEVAGKACC TGTFEMSYLC+QEN  TC DA KYVFWD
Sbjct: 263 TMLFSNPYAIFYQIITQPYLYGFEVAGKACCGTGTFEMSYLCSQENSLTCSDATKYVFWD 322

Query: 301 AFHPTQKTNQIIVNDLLRILLPMFR 326
           AFHPT+KTNQIIVNDLL+ LLP FR
Sbjct: 323 AFHPTEKTNQIIVNDLLQTLLPTFR 347

[Close]

BLAST of MS006372 vs. ExPASy TrEMBL
Match: A0A6J1JJ45 (GDSL esterase/lipase At2g42990-like OS=Cucurbita maxima OX=3661 GN=LOC111486280 PE=3 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 1.6e-164
Identity = 278/325 (85.54%), Postives = 299/325 (92.00%), Query Frame = 0


Query: 1   VPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNGRVPPDFISEAFGL 60
           VPAIIVFGDSSVDSGNNN +KT+LKSNF PYGRDF GGQ TGRFSNGRVPPDFISEAFGL
Sbjct: 23  VPAIIVFGDSSVDSGNNNAIKTLLKSNFMPYGRDFYGGQPTGRFSNGRVPPDFISEAFGL 82

Query: 61  KSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNVIPLWREVEFFKDYQNKLRAY 120
           K TIPAYLDP +TIADFATGVCFASAGTGFDN+TSDVLNVIPLWREVE +K+YQ KLR Y
Sbjct: 83  KPTIPAYLDPAFTIADFATGVCFASAGTGFDNATSDVLNVIPLWREVELYKEYQAKLRGY 142

Query: 121 MGNEKANEVIREALYLVSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGNFIGELYG 180
           +GNEKANEVI+EALYLVSLGTNDFLENYYTIPRRRLQF++QQYEDFLL+LAG FI E+Y 
Sbjct: 143 VGNEKANEVIKEALYLVSLGTNDFLENYYTIPRRRLQFSVQQYEDFLLQLAGKFIREIYS 202

Query: 181 LGARKISFTGLPPMGCLPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNRQLPGL 240
           LG RKIS +GLPPMGCLPLERATNVM NF CVEK+N VALEFN KLEGFV ALNRQLPGL
Sbjct: 203 LGVRKISISGLPPMGCLPLERATNVMSNFECVEKFNLVALEFNAKLEGFVWALNRQLPGL 262

Query: 241 TMLFANPFPIFYQIISQPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWD 300
           TMLF+NP+ IFYQII++PYLYGFEVAGKACC TGTFEMSYLC+QEN  TC DA KYVFWD
Sbjct: 263 TMLFSNPYAIFYQIITKPYLYGFEVAGKACCGTGTFEMSYLCSQENSLTCSDATKYVFWD 322

Query: 301 AFHPTQKTNQIIVNDLLRILLPMFR 326
           AFHPT+KTNQIIVNDLL+ LLPMFR
Sbjct: 323 AFHPTEKTNQIIVNDLLQTLLPMFR 347

[Close]

BLAST of MS006372 vs. ExPASy TrEMBL
Match: A0A0A0KGK8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G421680 PE=3 SV=1)

HSP 1 Score: 577.8 bits (1488), Expect = 2.9e-161
Identity = 271/324 (83.64%), Postives = 296/324 (91.36%), Query Frame = 0


Query: 1   VPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNGRVPPDFISEAFGL 60
           +PA+IVFGDSSVDSGNNNV+KT+LKSNFRPYGRDF+ GQ TGRFSNG+VPPDFISEAFGL
Sbjct: 21  IPAVIVFGDSSVDSGNNNVIKTLLKSNFRPYGRDFLSGQPTGRFSNGKVPPDFISEAFGL 80

Query: 61  KSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNVIPLWREVEFFKDYQNKLRAY 120
           K TIPAYLDP +TIADFATGVCFASAGTGFDNSTSDVLNVIP+W+EVE FK+YQ KLR Y
Sbjct: 81  KPTIPAYLDPAFTIADFATGVCFASAGTGFDNSTSDVLNVIPMWKEVELFKEYQRKLRGY 140

Query: 121 MGNEKANEVIREALYLVSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGNFIGELYG 180
           +GNEKANEVI+EALYLVSLGTNDFLENYYT P+RRLQF+IQQ+EDFLL+LA NFI +L+ 
Sbjct: 141 LGNEKANEVIKEALYLVSLGTNDFLENYYTFPQRRLQFSIQQFEDFLLDLARNFIKQLHN 200

Query: 181 LGARKISFTGLPPMGCLPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNRQLPGL 240
            GARKISFTGLPPMGCLPLERATNVMGNF CV+KYN VALEFN KLE FV  LN QLPGL
Sbjct: 201 DGARKISFTGLPPMGCLPLERATNVMGNFDCVDKYNLVALEFNNKLEAFVSDLNTQLPGL 260

Query: 241 TMLFANPFPIFYQIISQPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWD 300
           TM+F+NP+PIFYQII+ PYL+G+EVAGKACC TGTFEMSYLCNQEN FTC DANKYVFWD
Sbjct: 261 TMIFSNPYPIFYQIITNPYLFGYEVAGKACCGTGTFEMSYLCNQENSFTCPDANKYVFWD 320

Query: 301 AFHPTQKTNQIIVNDLLRILLPMF 325
           AFHPTQKTNQIIVN LL  LL  F
Sbjct: 321 AFHPTQKTNQIIVNHLLPSLLSTF 344

[Close]

BLAST of MS006372 vs. ExPASy TrEMBL
Match: A0A1S3BMS5 (GDSL esterase/lipase At2g42990-like OS=Cucumis melo OX=3656 GN=LOC103491363 PE=3 SV=1)

HSP 1 Score: 567.4 bits (1461), Expect = 3.9e-158
Identity = 269/325 (82.77%), Postives = 292/325 (89.85%), Query Frame = 0


Query: 1   VPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNGRVPPDFISEAFGL 60
           +PA+IVFGDSSVDSGNNNV+KT+LKSNFRPYGRDF+ GQ TGRFSNG+VPPDFISEAFGL
Sbjct: 22  IPAVIVFGDSSVDSGNNNVIKTLLKSNFRPYGRDFLTGQPTGRFSNGKVPPDFISEAFGL 81

Query: 61  KSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNVIPLWREVEFFKDYQNKLRAY 120
           K T+PAYLDP +TIADFATGVCFASAGTGFDNSTSDVLNVIPLW+EVE FK+YQ KLR Y
Sbjct: 82  KPTVPAYLDPAFTIADFATGVCFASAGTGFDNSTSDVLNVIPLWKEVELFKEYQRKLRGY 141

Query: 121 MGNEKANEVIREALYLVSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGNFIGELYG 180
           +GNEKANEVI+EALYLVSLGTNDFLENYYT P RRLQF+IQQ+EDFLL+LA NFI +LY 
Sbjct: 142 LGNEKANEVIKEALYLVSLGTNDFLENYYTFPHRRLQFSIQQFEDFLLDLARNFIKQLYH 201

Query: 181 LGARKISFTGLPPMGCLPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNRQLPGL 240
            GARK SFTGLPPMGCLPLERATNVM NF CV+KYN VALEFN KLE FV  LN QLPGL
Sbjct: 202 DGARKFSFTGLPPMGCLPLERATNVMSNFDCVDKYNLVALEFNNKLEAFVSDLNTQLPGL 261

Query: 241 TMLFANPFPIFYQIISQPYLYGFEVAGKACCATGTFEMSYLCN-QENPFTCMDANKYVFW 300
           TM+F+NP+PIFYQII+ PYL+G+E AGKACC TGTFEMSYLCN QEN FTC DANKYVFW
Sbjct: 262 TMIFSNPYPIFYQIITNPYLFGYEEAGKACCGTGTFEMSYLCNDQENSFTCPDANKYVFW 321

Query: 301 DAFHPTQKTNQIIVNDLLRILLPMF 325
           DAFHPTQKTNQIIVN LL  LL  F
Sbjct: 322 DAFHPTQKTNQIIVNHLLPSLLSTF 346

[Close]

BLAST of MS006372 vs. TAIR 10
Match: AT2G42990.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 458.4 bits (1178), Expect = 4.9e-129
Identity = 203/324 (62.65%), Postives = 261/324 (80.56%), Query Frame = 0


Query: 1   VPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNGRVPPDFISEAFGL 60
           +PAIIVFGDSSVDSGNNN + T+ ++NF PYGRDF GG+ATGRF NGR+  DF SEA+GL
Sbjct: 26  IPAIIVFGDSSVDSGNNNFISTMARANFEPYGRDFPGGRATGRFCNGRLSSDFTSEAYGL 85

Query: 61  KSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNVIPLWREVEFFKDYQNKLRAY 120
           K T+PAYLDP Y I+DFATGVCFASAGTG+DNST+DVL VIPLW+EVE+FK+YQ+ L AY
Sbjct: 86  KPTVPAYLDPSYNISDFATGVCFASAGTGYDNSTADVLGVIPLWKEVEYFKEYQSNLSAY 145

Query: 121 MGNEKANEVIREALYLVSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGNFIGELYG 180
           +G+ +A ++IRE+LY+VS+GTNDFLENYYT+P RR QF+I QY+DFL+E+A  F+ ++Y 
Sbjct: 146 LGHRRAAKIIRESLYIVSIGTNDFLENYYTLPDRRSQFSISQYQDFLVEIAEVFLKDIYR 205

Query: 181 LGARKISFTGLPPMGCLPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNRQLPGL 240
           LGARK+SFTG+ PMGCLPLER TN+   F C   YN +A++FN +L   V  LNR+L G+
Sbjct: 206 LGARKMSFTGISPMGCLPLERVTNLDDPFSCARSYNDLAVDFNGRLRRLVTKLNRELTGI 265

Query: 241 TMLFANPFPIFYQIISQPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWD 300
            + FANP+ I + I+++P LYG E++  ACC TG FEM +LC Q+NP TC DANK+VFWD
Sbjct: 266 KIYFANPYDIMWDIVTKPNLYGLEISSSACCGTGLFEMGFLCGQDNPLTCSDANKFVFWD 325

Query: 301 AFHPTQKTNQIIVNDLLRILLPMF 325
           AFHPT++TNQI+ +   + L  +F
Sbjct: 326 AFHPTERTNQIVSDHFFKHLKNLF 349

[Close]

BLAST of MS006372 vs. TAIR 10
Match: AT4G26790.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 440.3 bits (1131), Expect = 1.4e-123
Identity = 196/324 (60.49%), Postives = 258/324 (79.63%), Query Frame = 0


Query: 2   PAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNGRVPPDFISEAFGLK 61
           PA+IVFGDS+VDSGNNN + TVLKSNF+PYGRD+  G+ATGRFSNGR+ PDFISE  GLK
Sbjct: 28  PALIVFGDSTVDSGNNNQISTVLKSNFQPYGRDYFDGKATGRFSNGRIAPDFISEGLGLK 87

Query: 62  STIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNVIPLWREVEFFKDYQNKLRAYM 121
           + +PAYLDP Y IADFATGVCFASAGTG DN+TS VL+V+PLW+EVE++K+YQ +LR+Y+
Sbjct: 88  NAVPAYLDPAYNIADFATGVCFASAGTGLDNATSAVLSVMPLWKEVEYYKEYQTRLRSYL 147

Query: 122 GNEKANEVIREALYLVSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGNFIGELYGL 181
           G EKANE+I E+LYL+S+GTNDFLENYY +PR+  ++++ +Y+ FL+ +A +F+ ++Y L
Sbjct: 148 GEEKANEIISESLYLISIGTNDFLENYYLLPRKLRKYSVNEYQYFLIGIAADFVTDIYRL 207

Query: 182 GARKISFTGLPPMGCLPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNRQLPGLT 241
           GARK+S +GL P GCLPLER T +     C+E+YN VA +FN K+E  V  LNR L G+ 
Sbjct: 208 GARKMSLSGLSPFGCLPLERTTQLFYGSKCIEEYNIVARDFNIKMEEKVFQLNRDLNGIQ 267

Query: 242 MLFANPFPIFYQIISQPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWDA 301
           ++F+NP+ +  +II  P  +GFE    ACC TG +EMSYLC++ NPFTC DA+KYVFWD+
Sbjct: 268 LVFSNPYDLVSEIIYHPEAFGFENVRSACCGTGYYEMSYLCDKMNPFTCSDASKYVFWDS 327

Query: 302 FHPTQKTNQIIVNDLLRILLPMFR 326
           FHPT+KTN I+ N +L+  L  F+
Sbjct: 328 FHPTEKTNAIVANHVLKYDLSRFQ 351

[Close]

BLAST of MS006372 vs. TAIR 10
Match: AT4G26790.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 440.3 bits (1131), Expect = 1.4e-123
Identity = 196/324 (60.49%), Postives = 258/324 (79.63%), Query Frame = 0


Query: 2   PAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNGRVPPDFISEAFGLK 61
           PA+IVFGDS+VDSGNNN + TVLKSNF+PYGRD+  G+ATGRFSNGR+ PDFISE  GLK
Sbjct: 28  PALIVFGDSTVDSGNNNQISTVLKSNFQPYGRDYFDGKATGRFSNGRIAPDFISEGLGLK 87

Query: 62  STIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNVIPLWREVEFFKDYQNKLRAYM 121
           + +PAYLDP Y IADFATGVCFASAGTG DN+TS VL+V+PLW+EVE++K+YQ +LR+Y+
Sbjct: 88  NAVPAYLDPAYNIADFATGVCFASAGTGLDNATSAVLSVMPLWKEVEYYKEYQTRLRSYL 147

Query: 122 GNEKANEVIREALYLVSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGNFIGELYGL 181
           G EKANE+I E+LYL+S+GTNDFLENYY +PR+  ++++ +Y+ FL+ +A +F+ ++Y L
Sbjct: 148 GEEKANEIISESLYLISIGTNDFLENYYLLPRKLRKYSVNEYQYFLIGIAADFVTDIYRL 207

Query: 182 GARKISFTGLPPMGCLPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNRQLPGLT 241
           GARK+S +GL P GCLPLER T +     C+E+YN VA +FN K+E  V  LNR L G+ 
Sbjct: 208 GARKMSLSGLSPFGCLPLERTTQLFYGSKCIEEYNIVARDFNIKMEEKVFQLNRDLNGIQ 267

Query: 242 MLFANPFPIFYQIISQPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWDA 301
           ++F+NP+ +  +II  P  +GFE    ACC TG +EMSYLC++ NPFTC DA+KYVFWD+
Sbjct: 268 LVFSNPYDLVSEIIYHPEAFGFENVRSACCGTGYYEMSYLCDKMNPFTCSDASKYVFWDS 327

Query: 302 FHPTQKTNQIIVNDLLRILLPMFR 326
           FHPT+KTN I+ N +L+  L  F+
Sbjct: 328 FHPTEKTNAIVANHVLKYDLSRFQ 351

[Close]

BLAST of MS006372 vs. TAIR 10
Match: AT2G04570.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 435.3 bits (1118), Expect = 4.4e-122
Identity = 194/324 (59.88%), Postives = 250/324 (77.16%), Query Frame = 0


Query: 1   VPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNGRVPPDFISEAFGL 60
           +PAIIVFGDSSVD+GNNN + TV +SNF PYGRDFVGG+ TGRF NG++  DF+SEA GL
Sbjct: 26  IPAIIVFGDSSVDAGNNNYIPTVARSNFEPYGRDFVGGKPTGRFCNGKIATDFMSEALGL 85

Query: 61  KSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNVIPLWREVEFFKDYQNKLRAY 120
           K  IPAYLDP Y I+DFATGV FASA TG+DN+TSDVL+V+PLW+++E++K+YQ KL+AY
Sbjct: 86  KPIIPAYLDPSYNISDFATGVTFASAATGYDNATSDVLSVLPLWKQLEYYKEYQTKLKAY 145

Query: 121 MGNEKANEVIREALYLVSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGNFIGELYG 180
            G ++  E I  +LYL+S+GTNDFLENY+  P R  Q+++  Y+DFL  +A  F+ +L+G
Sbjct: 146 QGKDRGTETIESSLYLISIGTNDFLENYFAFPGRSSQYSVSLYQDFLAGIAKEFVKKLHG 205

Query: 181 LGARKISFTGLPPMGCLPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNRQLPGL 240
           LGARKIS  GLPPMGC+PLERATN+     CV +YN +A++FN KL+  V  L+++LPG 
Sbjct: 206 LGARKISLGGLPPMGCMPLERATNIGTGGECVGRYNDIAVQFNSKLDKMVEKLSKELPGS 265

Query: 241 TMLFANPFPIFYQIISQPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWD 300
            ++F+NP+  F +II  P  +GFEV G ACCATG FEM Y C + NPFTC +A+KYVFWD
Sbjct: 266 NLVFSNPYEPFMRIIKNPSSFGFEVVGAACCATGMFEMGYGCQRNNPFTCTNADKYVFWD 325

Query: 301 AFHPTQKTNQIIVNDLLRILLPMF 325
           +FHPTQKTN I+ N L+    P F
Sbjct: 326 SFHPTQKTNHIMANALMNSTFPHF 349

[Close]

BLAST of MS006372 vs. TAIR 10
Match: AT5G42170.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 292.4 bits (747), Expect = 4.6e-79
Identity = 140/317 (44.16%), Postives = 198/317 (62.46%), Query Frame = 0


Query: 1   VPAIIVFGDSSVDSGNNNVVKTVLKSNFRPYGRDFVGGQATGRFSNGRVPPDFISEAFGL 60
           +P II FGDS VDSGNNN ++T LK NF PYG+DF G  ATGRFS+GRVP D ++E  G+
Sbjct: 48  IPGIITFGDSIVDSGNNNHLRTALKCNFPPYGKDFPGKIATGRFSDGRVPSDIVAERLGI 107

Query: 61  KSTIPAYLDPGYTIADFATGVCFASAGTGFDNSTSDVLNVIPLWREVEFFKDYQNKLRAY 120
             TIPAYL+P     D   GV FAS G+G+D  T+ ++ V+ L  +++ F++Y+NKL+  
Sbjct: 108 AETIPAYLNPKLKNEDLLKGVNFASGGSGYDPLTAKLVKVVSLSDQLKNFQEYKNKLKVI 167

Query: 121 MGNEKANEVIREALYLVSLGTNDFLENYYTIPRRRLQFTIQQYEDFLLELAGNFIGELYG 180
           +G EKAN +++ +LYLV   +ND    Y     R +++    Y D+L + A  F+  LYG
Sbjct: 168 VGEEKANFLVKNSLYLVVASSNDIAHTY---TARSIKYNKTSYADYLADSASKFVSALYG 227

Query: 181 LGARKISFTGLPPMGCLPLERATNVMGNFGCVEKYNAVALEFNRKLEGFVGALNRQLPGL 240
           LGAR+I      P+GC+P  R         C EK N VA  FN K+   + AL ++LP  
Sbjct: 228 LGARRIGVFSAVPVGCVPAARTLRGKLKRRCSEKLNEVARNFNAKISPTLEALGKELPDS 287

Query: 241 TMLFANPFPIFYQIISQPYLYGFEVAGKACCATGTFEMSYLCNQENPFTCMDANKYVFWD 300
            ++  +       +I  P  YGFEV+ + CC TG  E+ +LCN+ NPFTC +++ Y+FWD
Sbjct: 288 RVVLIDVCDTLNDMIENPKNYGFEVSNRGCCGTGLVEVLFLCNKINPFTCKNSSSYIFWD 347

Query: 301 AFHPTQKTNQIIVNDLL 318
           ++HPT+K  QIIV+ LL
Sbjct: 348 SYHPTEKAYQIIVDKLL 361

The following BLAST results are available for this feature:

BLAST of MS006372 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147832.1	1.1e-188	99.38	GDSL esterase/lipase At2g42990-like isoform X1 [Momordica charantia]	[more]
XP_022930712.1	2.3e-165	85.85	GDSL esterase/lipase At2g42990-like [Cucurbita moschata]	[more]
XP_023529877.1	3.0e-165	85.85	GDSL esterase/lipase At2g42990-like [Cucurbita pepo subsp. pepo]	[more]
KAG6589280.1	5.2e-165	85.54	GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022989111.1	3.4e-164	85.54	GDSL esterase/lipase At2g42990-like [Cucurbita maxima]	[more]

BLAST of MS006372 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q67ZI9	6.9e-128	62.65	GDSL esterase/lipase At2g42990 OS=Arabidopsis thaliana OX=3702 GN=At2g42990 PE=2...	[more]
Q8VY93	1.9e-122	60.49	GDSL esterase/lipase At4g26790 OS=Arabidopsis thaliana OX=3702 GN=At4g26790 PE=2...	[more]
Q9SJB4	6.2e-121	59.88	GDSL esterase/lipase At2g04570 OS=Arabidopsis thaliana OX=3702 GN=At2g04570 PE=2...	[more]
Q9FHW9	6.5e-78	44.16	GDSL esterase/lipase At5g42170 OS=Arabidopsis thaliana OX=3702 GN=At5g42170/At5g...	[more]
Q9C653	1.1e-77	43.13	GDSL esterase/lipase At1g58525 OS=Arabidopsis thaliana OX=3702 GN=At1g58525 PE=3...	[more]

BLAST of MS006372 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D3F6	5.5e-189	99.38	GDSL esterase/lipase At2g42990-like isoform X1 OS=Momordica charantia OX=3673 GN...	[more]
A0A6J1ERD0	1.1e-165	85.85	GDSL esterase/lipase At2g42990-like OS=Cucurbita moschata OX=3662 GN=LOC11143711...	[more]
A0A6J1JJ45	1.6e-164	85.54	GDSL esterase/lipase At2g42990-like OS=Cucurbita maxima OX=3661 GN=LOC111486280 ...	[more]
A0A0A0KGK8	2.9e-161	83.64	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G421680 PE=3 SV=1	[more]
A0A1S3BMS5	3.9e-158	82.77	GDSL esterase/lipase At2g42990-like OS=Cucumis melo OX=3656 GN=LOC103491363 PE=3...	[more]

BLAST of MS006372 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G42990.1	4.9e-129	62.65	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT4G26790.1	1.4e-123	60.49	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT4G26790.2	1.4e-123	60.49	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT2G04570.1	4.4e-122	59.88	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT5G42170.1	4.6e-79	44.16	SGNH hydrolase-type esterase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 1..321 e-value: 1.4E-70 score: 240.2
IPR001087	GDSL lipase/esterase	PFAM	PF00657	Lipase_GDSL	coord: 4..314 e-value: 3.7E-34 score: 119.1
None	No IPR available	PANTHER	PTHR45642	GDSL ESTERASE/LIPASE EXL3	coord: 1..324
None	No IPR available	PANTHER	PTHR45642:SF72	GDSL-LIKE LIPASE/ACYLHYDROLASE	coord: 1..324
None	No IPR available	SUPERFAMILY	52266	SGNH hydrolase	coord: 4..320
IPR035669	GDSL lipase/esterase-like, plant	CDD	cd01837	SGNH_plant_lipase_like	coord: 2..317 e-value: 1.52774E-139 score: 395.831

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS006372.1	MS006372.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds