MS006336 (gene) Bitter gourd (TR) v1

Overview
NameMS006336
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionProtein kinase domain-containing protein
Locationscaffold123_1: 229582 .. 232178 (+)
RNA-Seq ExpressionMS006336
SyntenyMS006336
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCTGCATTTGCTCAAGAAGTTCCTCAGAACCAGAGGATAGCAATCAGAAAAAGTCTGAAAAAGATTTGAACAAATCTTCAGTTCAGTTGGTTGCTCCTTCTCCATCAAAAGGGGAGGATTTGCTATTGGAGATTCAGTTGTCAAGGCAGGCATCTAAAGTAAAGGGGGGTTCTGTTCATAAAACAAAGGCCAATGGAGGGTCTCACCACCATAGAGCAAAGGATGATGAAGCTGAGAATAAATCAGTAAAAGCTGGAAAAGCAAATAATGATAATGCTTCAACAGGTGGGAAACAGCCTGAGGTTTTTAGGTTGGCTAGCTTGTCTAAAGCGGCTGAGACTGAACTTGTAATGGCTGGATGGCCTTCGTGGTTGGCTTCGGTTGCCGGAGAAGCCATCAAGGGGTGGGTGCCTCGTCGGGCTGACTCCTTTGAGAAGTTAGACAAAGTTAGTTCCCGTTATAACATTTCCTTTTACTTCAATTCTTTAGAATCAGATGACTCTAATGCATATATGTATCTATTGAACAGCAACTGATTGCAATCTAATCGATGTTCTAAACACCATCACCATGTATTTGCCTTCAAATGAGTTTCTCACAAATGGTTTTAACTATTGACCCCTCCCCTCATTGGTCAAGTGCTATTGTCCTTCTATTTTTATTGATAATGCAATGCTCTCTCTTTCTTTTGGGTGAAGTTATTTTGTGTATGTTACATATGTGTGGGGAACCAAAACGTTACTGGCCATGGGTGATGGGGAGAAATTATTTGAGGTTGCTGAAACAATGTGGCTGAAAACTTTATATCCATATGCGTCTACTAGGTTTCAGAGACATCAAGGATTTTTCTTTGTTAAGTTTATTAAAAAATTTGGACATGTGACCTTTGGGTAGGTGTTACTTAGAGACTTAGGGCCCTAGCCAACCAGGCCACCCCTTGGGAGTATATGGAGAATTTTAAAACTGCATTTTGAGCCTCTATCAACATAGATTTTTAAACTGCATTGTGGCTGCCTCATTGAATAATTTTCATCAGAGGGCTACTCGGGGGGGAATGTAGATAGGAATCCCAGTTAAAAGACTAGTCCGTTTTGAGTATCATACAACTTCTTCTGTGCATCTCTCTCTCATTATACATCAGTATGTGCTGATTATCATATTATGTTCTTATATGCAGATCGGACAGGGAACTTATAGCAGTGTGTATAAAGCCCGTGACCTTGAAACCGGTAAAACTGTGGCATTGAAAAAAGTGAGATTTGTAAATATGGATCCAGAAAGTGTTCGTTTTATGGCTAGGGAAATATATATTTTGCGTAAACTTGATCATCCAAACGTTATGAAGCTCGAGTGTCTAGTCACTTCGAGAGTGTCAGGCAGCTTGTACCTTGTTTTTGAATACATGGAGCATGACCTTGCAGGTCTCGCAGCAGCTCCTGGACATAAGTTTACTGAGGCTCAGGTATCTTCTTCTTGGCTTGCTTGAGCATTTCAGTTTTTTTTTTGTCTTGAACTGGAACCAACGTGAAAATGAATGCTTATTCAAATTGAGCCATCTATGAAAACTGAAGGAAGTGATGGAGATATTTGAAACCATTGTAATTGAAGTGTCATTTTGTGCCTGCAGATTAAATGTTTTGTGCAACAATTGCTGCATGGACTCGAACACTGTCATAGTCGGGGAGTTCTGCACCGGGATATCAAAGGTTCAAATCTTCTCGTTGACAGTAATGGTGTGCTCAAGATTGGAGACTTTGGTCTTGCTACCTTTTTTAAGCCTGAACAAAAGCAGCCATTGACGAGTCGGGTAGTGACGTTGTGGTACAGGGCACCCGAGCTTTTGCTCGGTGCTACTGATTATGGACCTGCCATTGATCTCTGGAGCTGTGGCTGTATCGTCGCTGAACTATTTGCTGGAAAACCTATTATGCCAGGAAGAACAGAGGTTTAATCATCTACTCGTTAGCCTGTTTTGTTTTTTGGTAATCTGTAAGAACATAAGAGAAAACAATAAGTTGTTAGTTTGCATGAACAGTATATAGATATAAACCAAACGATTTTGTCATTAATTCTTCGTACATGCTCTAGAAATCATCATAAAAGTAATTGGAAGTTGTTGAATCGTTGAGTTTTTCTCAGGTGGAACAAATGCACAAGATTTTCAAGCTCTGTGGTTCACCATCTGAGGAGTTCTGGCGGAGAACAAAATTGCCACATGCAACCAGCTTCAAACCTCATCACCCTTATAAAAGCTGCCTTTCTGAGACGTTTAAAAATTTCCCTTCTTCAGCTTTGGCTCTTGTAAATAAACTACTTGCCATAGAACCAGAGCACAGAGGATCAGCTACTTCAGCTCTCAGCAGTGAGGTATTTTGAAGTTTCTTTTGTATGTCTAGGACCATCTACTTTACTCTCTGCTCTATTTTCTTCTTGATGTTGTCCTTTCTGATTATGTTGCTTGCATATCATAGTAACCTATCTGGTTTTGCAGTTTTTCATAACGGAACCTCTCCCTTGTGACCCTTCAAGCTTACCAAAATATCCTCCGAGCAAGGAATACGATGCAAAGCTTCGGAACGAAGAAGAGAGAAAG

mRNA sequence

ATGGGCTGCATTTGCTCAAGAAGTTCCTCAGAACCAGAGGATAGCAATCAGAAAAAGTCTGAAAAAGATTTGAACAAATCTTCAGTTCAGTTGGTTGCTCCTTCTCCATCAAAAGGGGAGGATTTGCTATTGGAGATTCAGTTGTCAAGGCAGGCATCTAAAGTAAAGGGGGGTTCTGTTCATAAAACAAAGGCCAATGGAGGGTCTCACCACCATAGAGCAAAGGATGATGAAGCTGAGAATAAATCAGTAAAAGCTGGAAAAGCAAATAATGATAATGCTTCAACAGGTGGGAAACAGCCTGAGGTTTTTAGGTTGGCTAGCTTGTCTAAAGCGGCTGAGACTGAACTTGTAATGGCTGGATGGCCTTCGTGGTTGGCTTCGGTTGCCGGAGAAGCCATCAAGGGGTGGGTGCCTCGTCGGGCTGACTCCTTTGAGAAGTTAGACAAAATCGGACAGGGAACTTATAGCAGTGTGTATAAAGCCCGTGACCTTGAAACCGGTAAAACTGTGGCATTGAAAAAAGTGAGATTTGTAAATATGGATCCAGAAAGTGTTCGTTTTATGGCTAGGGAAATATATATTTTGCGTAAACTTGATCATCCAAACGTTATGAAGCTCGAGTGTCTAGTCACTTCGAGAGTGTCAGGCAGCTTGTACCTTGTTTTTGAATACATGGAGCATGACCTTGCAGGTCTCGCAGCAGCTCCTGGACATAAGTTTACTGAGGCTCAGATTAAATGTTTTGTGCAACAATTGCTGCATGGACTCGAACACTGTCATAGTCGGGGAGTTCTGCACCGGGATATCAAAGGTTCAAATCTTCTCGTTGACAGTAATGGTGTGCTCAAGATTGGAGACTTTGGTCTTGCTACCTTTTTTAAGCCTGAACAAAAGCAGCCATTGACGAGTCGGGTAGTGACGTTGTGGTACAGGGCACCCGAGCTTTTGCTCGGTGCTACTGATTATGGACCTGCCATTGATCTCTGGAGCTGTGGCTGTATCGTCGCTGAACTATTTGCTGGAAAACCTATTATGCCAGGAAGAACAGAGGTGGAACAAATGCACAAGATTTTCAAGCTCTGTGGTTCACCATCTGAGGAGTTCTGGCGGAGAACAAAATTGCCACATGCAACCAGCTTCAAACCTCATCACCCTTATAAAAGCTGCCTTTCTGAGACGTTTAAAAATTTCCCTTCTTCAGCTTTGGCTCTTGTAAATAAACTACTTGCCATAGAACCAGAGCACAGAGGATCAGCTACTTCAGCTCTCAGCAGTGAGTTTTTCATAACGGAACCTCTCCCTTGTGACCCTTCAAGCTTACCAAAATATCCTCCGAGCAAGGAATACGATGCAAAGCTTCGGAACGAAGAAGAGAGAAAG

Coding sequence (CDS)

ATGGGCTGCATTTGCTCAAGAAGTTCCTCAGAACCAGAGGATAGCAATCAGAAAAAGTCTGAAAAAGATTTGAACAAATCTTCAGTTCAGTTGGTTGCTCCTTCTCCATCAAAAGGGGAGGATTTGCTATTGGAGATTCAGTTGTCAAGGCAGGCATCTAAAGTAAAGGGGGGTTCTGTTCATAAAACAAAGGCCAATGGAGGGTCTCACCACCATAGAGCAAAGGATGATGAAGCTGAGAATAAATCAGTAAAAGCTGGAAAAGCAAATAATGATAATGCTTCAACAGGTGGGAAACAGCCTGAGGTTTTTAGGTTGGCTAGCTTGTCTAAAGCGGCTGAGACTGAACTTGTAATGGCTGGATGGCCTTCGTGGTTGGCTTCGGTTGCCGGAGAAGCCATCAAGGGGTGGGTGCCTCGTCGGGCTGACTCCTTTGAGAAGTTAGACAAAATCGGACAGGGAACTTATAGCAGTGTGTATAAAGCCCGTGACCTTGAAACCGGTAAAACTGTGGCATTGAAAAAAGTGAGATTTGTAAATATGGATCCAGAAAGTGTTCGTTTTATGGCTAGGGAAATATATATTTTGCGTAAACTTGATCATCCAAACGTTATGAAGCTCGAGTGTCTAGTCACTTCGAGAGTGTCAGGCAGCTTGTACCTTGTTTTTGAATACATGGAGCATGACCTTGCAGGTCTCGCAGCAGCTCCTGGACATAAGTTTACTGAGGCTCAGATTAAATGTTTTGTGCAACAATTGCTGCATGGACTCGAACACTGTCATAGTCGGGGAGTTCTGCACCGGGATATCAAAGGTTCAAATCTTCTCGTTGACAGTAATGGTGTGCTCAAGATTGGAGACTTTGGTCTTGCTACCTTTTTTAAGCCTGAACAAAAGCAGCCATTGACGAGTCGGGTAGTGACGTTGTGGTACAGGGCACCCGAGCTTTTGCTCGGTGCTACTGATTATGGACCTGCCATTGATCTCTGGAGCTGTGGCTGTATCGTCGCTGAACTATTTGCTGGAAAACCTATTATGCCAGGAAGAACAGAGGTGGAACAAATGCACAAGATTTTCAAGCTCTGTGGTTCACCATCTGAGGAGTTCTGGCGGAGAACAAAATTGCCACATGCAACCAGCTTCAAACCTCATCACCCTTATAAAAGCTGCCTTTCTGAGACGTTTAAAAATTTCCCTTCTTCAGCTTTGGCTCTTGTAAATAAACTACTTGCCATAGAACCAGAGCACAGAGGATCAGCTACTTCAGCTCTCAGCAGTGAGTTTTTCATAACGGAACCTCTCCCTTGTGACCCTTCAAGCTTACCAAAATATCCTCCGAGCAAGGAATACGATGCAAAGCTTCGGAACGAAGAAGAGAGAAAG

Protein sequence

MGCICSRSSSEPEDSNQKKSEKDLNKSSVQLVAPSPSKGEDLLLEIQLSRQASKVKGGSVHKTKANGGSHHHRAKDDEAENKSVKAGKANNDNASTGGKQPEVFRLASLSKAAETELVMAGWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGKTVALKKVRFVNMDPESVRFMAREIYILRKLDHPNVMKLECLVTSRVSGSLYLVFEYMEHDLAGLAAAPGHKFTEAQIKCFVQQLLHGLEHCHSRGVLHRDIKGSNLLVDSNGVLKIGDFGLATFFKPEQKQPLTSRVVTLWYRAPELLLGATDYGPAIDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFKLCGSPSEEFWRRTKLPHATSFKPHHPYKSCLSETFKNFPSSALALVNKLLAIEPEHRGSATSALSSEFFITEPLPCDPSSLPKYPPSKEYDAKLRNEEERK
Homology
BLAST of MS006336 vs. NCBI nr
Match: XP_022141899.1 (probable serine/threonine-protein kinase At1g09600 [Momordica charantia])

HSP 1 Score: 922.9 bits (2384), Expect = 1.1e-264
Identity = 461/461 (100.00%), Postives = 461/461 (100.00%), Query Frame = 0

Query: 1   MGCICSRSSSEPEDSNQKKSEKDLNKSSVQLVAPSPSKGEDLLLEIQLSRQASKVKGGSV 60
           MGCICSRSSSEPEDSNQKKSEKDLNKSSVQLVAPSPSKGEDLLLEIQLSRQASKVKGGSV
Sbjct: 1   MGCICSRSSSEPEDSNQKKSEKDLNKSSVQLVAPSPSKGEDLLLEIQLSRQASKVKGGSV 60

Query: 61  HKTKANGGSHHHRAKDDEAENKSVKAGKANNDNASTGGKQPEVFRLASLSKAAETELVMA 120
           HKTKANGGSHHHRAKDDEAENKSVKAGKANNDNASTGGKQPEVFRLASLSKAAETELVMA
Sbjct: 61  HKTKANGGSHHHRAKDDEAENKSVKAGKANNDNASTGGKQPEVFRLASLSKAAETELVMA 120

Query: 121 GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGKTVALKKVRFVN 180
           GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGKTVALKKVRFVN
Sbjct: 121 GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGKTVALKKVRFVN 180

Query: 181 MDPESVRFMAREIYILRKLDHPNVMKLECLVTSRVSGSLYLVFEYMEHDLAGLAAAPGHK 240
           MDPESVRFMAREIYILRKLDHPNVMKLECLVTSRVSGSLYLVFEYMEHDLAGLAAAPGHK
Sbjct: 181 MDPESVRFMAREIYILRKLDHPNVMKLECLVTSRVSGSLYLVFEYMEHDLAGLAAAPGHK 240

Query: 241 FTEAQIKCFVQQLLHGLEHCHSRGVLHRDIKGSNLLVDSNGVLKIGDFGLATFFKPEQKQ 300
           FTEAQIKCFVQQLLHGLEHCHSRGVLHRDIKGSNLLVDSNGVLKIGDFGLATFFKPEQKQ
Sbjct: 241 FTEAQIKCFVQQLLHGLEHCHSRGVLHRDIKGSNLLVDSNGVLKIGDFGLATFFKPEQKQ 300

Query: 301 PLTSRVVTLWYRAPELLLGATDYGPAIDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK 360
           PLTSRVVTLWYRAPELLLGATDYGPAIDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK
Sbjct: 301 PLTSRVVTLWYRAPELLLGATDYGPAIDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK 360

Query: 361 LCGSPSEEFWRRTKLPHATSFKPHHPYKSCLSETFKNFPSSALALVNKLLAIEPEHRGSA 420
           LCGSPSEEFWRRTKLPHATSFKPHHPYKSCLSETFKNFPSSALALVNKLLAIEPEHRGSA
Sbjct: 361 LCGSPSEEFWRRTKLPHATSFKPHHPYKSCLSETFKNFPSSALALVNKLLAIEPEHRGSA 420

Query: 421 TSALSSEFFITEPLPCDPSSLPKYPPSKEYDAKLRNEEERK 462
           TSALSSEFFITEPLPCDPSSLPKYPPSKEYDAKLRNEEERK
Sbjct: 421 TSALSSEFFITEPLPCDPSSLPKYPPSKEYDAKLRNEEERK 461

BLAST of MS006336 vs. NCBI nr
Match: XP_038889815.1 (probable serine/threonine-protein kinase At1g09600 isoform X1 [Benincasa hispida] >XP_038889816.1 probable serine/threonine-protein kinase At1g09600 isoform X1 [Benincasa hispida] >XP_038889818.1 probable serine/threonine-protein kinase At1g09600 isoform X1 [Benincasa hispida])

HSP 1 Score: 847.0 bits (2187), Expect = 7.4e-242
Identity = 420/461 (91.11%), Postives = 438/461 (95.01%), Query Frame = 0

Query: 1   MGCICSRSSSEPEDSNQKKSEKDLNKSSVQLVAPSPSKGEDLLLEIQLSRQASKVKGGSV 60
           MGCICSR SSEPEDSN K+SEKDLNKSSVQLVAPSPSK ED++LEIQLSRQASK  GGSV
Sbjct: 1   MGCICSRDSSEPEDSNHKESEKDLNKSSVQLVAPSPSKREDMMLEIQLSRQASKANGGSV 60

Query: 61  HKTKANGGSHHHRAKDDEAENKSVKAGKANNDNASTGGKQPEVFRLASLSKAAETELVMA 120
           HK KANGGS H   +DDE+ENKS+KA KANNDNAS GGKQP++FRLASLSK AE ELVMA
Sbjct: 61  HKAKANGGSVHRAKEDDESENKSIKAAKANNDNASAGGKQPDIFRLASLSKGAEAELVMA 120

Query: 121 GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGKTVALKKVRFVN 180
           GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGK VALKKVRFVN
Sbjct: 121 GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGKIVALKKVRFVN 180

Query: 181 MDPESVRFMAREIYILRKLDHPNVMKLECLVTSRVSGSLYLVFEYMEHDLAGLAAAPGHK 240
           MDPESVRFMAREIYILRKLDHPNVMKLE LVTSR+SGSLYLVFEYMEHDLAGLAAAPGHK
Sbjct: 181 MDPESVRFMAREIYILRKLDHPNVMKLESLVTSRMSGSLYLVFEYMEHDLAGLAAAPGHK 240

Query: 241 FTEAQIKCFVQQLLHGLEHCHSRGVLHRDIKGSNLLVDSNGVLKIGDFGLATFFKPEQKQ 300
           FTEAQIKC+VQQLLHGLEHCHSRG+LHRDIKGSNLLVD+NGVLKIGDFGLATFF+P++KQ
Sbjct: 241 FTEAQIKCYVQQLLHGLEHCHSRGILHRDIKGSNLLVDNNGVLKIGDFGLATFFQPDKKQ 300

Query: 301 PLTSRVVTLWYRAPELLLGATDYGPAIDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK 360
           PLTSRVVTLWYRAPELLLGAT YGPAIDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK
Sbjct: 301 PLTSRVVTLWYRAPELLLGATQYGPAIDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK 360

Query: 361 LCGSPSEEFWRRTKLPHATSFKPHHPYKSCLSETFKNFPSSALALVNKLLAIEPEHRGSA 420
           LCGSPSEEFW+RTKLPHATSFKP H YKSCLSETFK+FP SALALVNKLLAIEPEHRGSA
Sbjct: 361 LCGSPSEEFWQRTKLPHATSFKPQHRYKSCLSETFKSFPPSALALVNKLLAIEPEHRGSA 420

Query: 421 TSALSSEFFITEPLPCDPSSLPKYPPSKEYDAKLRNEEERK 462
           T AL SEFF TEPLPCDPSSLPKYPPSKE+DAKLRNEEERK
Sbjct: 421 TLALRSEFFRTEPLPCDPSSLPKYPPSKEFDAKLRNEEERK 461

BLAST of MS006336 vs. NCBI nr
Match: XP_011656099.1 (probable serine/threonine-protein kinase At1g09600 isoform X1 [Cucumis sativus])

HSP 1 Score: 842.0 bits (2174), Expect = 2.4e-240
Identity = 418/461 (90.67%), Postives = 435/461 (94.36%), Query Frame = 0

Query: 1   MGCICSRSSSEPEDSNQKKSEKDLNKSSVQLVAPSPSKGEDLLLEIQLSRQASKVKGGSV 60
           MGCICSR SSEPEDS+ K+ EK+LNKSSVQLVAPSPSK ED+LLEIQL+RQASK  GGSV
Sbjct: 1   MGCICSRDSSEPEDSSHKEPEKELNKSSVQLVAPSPSKREDMLLEIQLTRQASKANGGSV 60

Query: 61  HKTKANGGSHHHRAKDDEAENKSVKAGKANNDNASTGGKQPEVFRLASLSKAAETELVMA 120
           HK K NGGS H   +DDE+ENKSVKA KANNDNAS GGKQP++FRLASLSK AETELVMA
Sbjct: 61  HKAKVNGGSVHRAKEDDESENKSVKAAKANNDNASVGGKQPDIFRLASLSKGAETELVMA 120

Query: 121 GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGKTVALKKVRFVN 180
           GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGK VALKKVRFVN
Sbjct: 121 GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGKIVALKKVRFVN 180

Query: 181 MDPESVRFMAREIYILRKLDHPNVMKLECLVTSRVSGSLYLVFEYMEHDLAGLAAAPGHK 240
           MDPESVRFMAREIYILRKLDHPNVMKLE LVTSR+SGSLYLVFEYMEHDLAGLAA PGHK
Sbjct: 181 MDPESVRFMAREIYILRKLDHPNVMKLESLVTSRMSGSLYLVFEYMEHDLAGLAAVPGHK 240

Query: 241 FTEAQIKCFVQQLLHGLEHCHSRGVLHRDIKGSNLLVDSNGVLKIGDFGLATFFKPEQKQ 300
           FTEAQIKC+VQQLLHGLEHCHSRG+LHRDIKGSNLLVD+NGVLKIGDFGLATFF+P+QKQ
Sbjct: 241 FTEAQIKCYVQQLLHGLEHCHSRGILHRDIKGSNLLVDNNGVLKIGDFGLATFFQPDQKQ 300

Query: 301 PLTSRVVTLWYRAPELLLGATDYGPAIDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK 360
           PLTSRVVTLWYRAPELLLGAT YGP IDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK
Sbjct: 301 PLTSRVVTLWYRAPELLLGATQYGPGIDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK 360

Query: 361 LCGSPSEEFWRRTKLPHATSFKPHHPYKSCLSETFKNFPSSALALVNKLLAIEPEHRGSA 420
           LCGSPSEEFWRRTKLPHATSFKP H YKSCLSETFK+FP SALALVNKLLAIEPEHRGSA
Sbjct: 361 LCGSPSEEFWRRTKLPHATSFKPQHRYKSCLSETFKSFPPSALALVNKLLAIEPEHRGSA 420

Query: 421 TSALSSEFFITEPLPCDPSSLPKYPPSKEYDAKLRNEEERK 462
           T AL SEFF TEPLPCDPSSLPKYPPSKE+DAKLRNEEERK
Sbjct: 421 TLALRSEFFRTEPLPCDPSSLPKYPPSKEFDAKLRNEEERK 461

BLAST of MS006336 vs. NCBI nr
Match: KAA0039258.1 (putative serine/threonine-protein kinase [Cucumis melo var. makuwa] >TYK00444.1 putative serine/threonine-protein kinase [Cucumis melo var. makuwa])

HSP 1 Score: 839.3 bits (2167), Expect = 1.5e-239
Identity = 417/461 (90.46%), Postives = 436/461 (94.58%), Query Frame = 0

Query: 1   MGCICSRSSSEPEDSNQKKSEKDLNKSSVQLVAPSPSKGEDLLLEIQLSRQASKVKGGSV 60
           MGCICSR SSEPEDS+ K+ EK+LNKSSVQLVAPSPSK ED+LLEIQLSRQASK  GGSV
Sbjct: 1   MGCICSRDSSEPEDSSHKEPEKELNKSSVQLVAPSPSKREDMLLEIQLSRQASKANGGSV 60

Query: 61  HKTKANGGSHHHRAKDDEAENKSVKAGKANNDNASTGGKQPEVFRLASLSKAAETELVMA 120
           HK KANGGS H   +DDE+ENKS+KA KANNDNAS GGKQP++FRLASLSK AETELVMA
Sbjct: 61  HKAKANGGSVHRAKEDDESENKSLKAAKANNDNASVGGKQPDIFRLASLSKGAETELVMA 120

Query: 121 GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGKTVALKKVRFVN 180
           GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGK VALKKVRFVN
Sbjct: 121 GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGKIVALKKVRFVN 180

Query: 181 MDPESVRFMAREIYILRKLDHPNVMKLECLVTSRVSGSLYLVFEYMEHDLAGLAAAPGHK 240
           MDPESVRFMAREIYILRKLDHPNVMKLE LVTSR+SGSLYLVFEYMEHDLAGL+AAPGHK
Sbjct: 181 MDPESVRFMAREIYILRKLDHPNVMKLESLVTSRMSGSLYLVFEYMEHDLAGLSAAPGHK 240

Query: 241 FTEAQIKCFVQQLLHGLEHCHSRGVLHRDIKGSNLLVDSNGVLKIGDFGLATFFKPEQKQ 300
           FTEAQIKC+VQQLLHGLEHCHSRG+LHRDIKGSNLL+D+NGVLKIGDFGLATFF+P+QKQ
Sbjct: 241 FTEAQIKCYVQQLLHGLEHCHSRGILHRDIKGSNLLLDNNGVLKIGDFGLATFFQPDQKQ 300

Query: 301 PLTSRVVTLWYRAPELLLGATDYGPAIDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK 360
           PLTSRVVTLWYRAPELLLGAT YGP IDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK
Sbjct: 301 PLTSRVVTLWYRAPELLLGATQYGPGIDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK 360

Query: 361 LCGSPSEEFWRRTKLPHATSFKPHHPYKSCLSETFKNFPSSALALVNKLLAIEPEHRGSA 420
           LCGSPSEEFWRRTKLPHATSFKP   YKSCLSETFK+FP SALALVNKLLAIEPEHRGSA
Sbjct: 361 LCGSPSEEFWRRTKLPHATSFKPQSRYKSCLSETFKSFPPSALALVNKLLAIEPEHRGSA 420

Query: 421 TSALSSEFFITEPLPCDPSSLPKYPPSKEYDAKLRNEEERK 462
           T AL SEFF TEPLPCDPSSLPKYPPSKE+DAKLRNEEERK
Sbjct: 421 TLALRSEFFRTEPLPCDPSSLPKYPPSKEFDAKLRNEEERK 461

BLAST of MS006336 vs. NCBI nr
Match: XP_008459627.1 (PREDICTED: probable serine/threonine-protein kinase At1g09600 [Cucumis melo] >XP_008459630.1 PREDICTED: probable serine/threonine-protein kinase At1g09600 [Cucumis melo])

HSP 1 Score: 833.9 bits (2153), Expect = 6.5e-238
Identity = 415/461 (90.02%), Postives = 434/461 (94.14%), Query Frame = 0

Query: 1   MGCICSRSSSEPEDSNQKKSEKDLNKSSVQLVAPSPSKGEDLLLEIQLSRQASKVKGGSV 60
           MGCICSR SSEPEDS+ K+ EK+ NKSSVQLVAPSPSK ED+LLEIQLSRQASK  GGSV
Sbjct: 1   MGCICSRDSSEPEDSSHKEPEKEFNKSSVQLVAPSPSKREDMLLEIQLSRQASKANGGSV 60

Query: 61  HKTKANGGSHHHRAKDDEAENKSVKAGKANNDNASTGGKQPEVFRLASLSKAAETELVMA 120
           HK KANGGS H   +DDE+ENKS+KA KANNDNAS GGKQ ++FRLASLSK AETELVMA
Sbjct: 61  HKAKANGGSVHRAKEDDESENKSLKAAKANNDNASVGGKQLDIFRLASLSKGAETELVMA 120

Query: 121 GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGKTVALKKVRFVN 180
           GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGK VALKKVRFVN
Sbjct: 121 GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGKIVALKKVRFVN 180

Query: 181 MDPESVRFMAREIYILRKLDHPNVMKLECLVTSRVSGSLYLVFEYMEHDLAGLAAAPGHK 240
           MDPESVRFMAREIYILRKLDHPNVMKLE LVTSR+SGSLYLVFEYMEHDLAGL+AAPGHK
Sbjct: 181 MDPESVRFMAREIYILRKLDHPNVMKLESLVTSRMSGSLYLVFEYMEHDLAGLSAAPGHK 240

Query: 241 FTEAQIKCFVQQLLHGLEHCHSRGVLHRDIKGSNLLVDSNGVLKIGDFGLATFFKPEQKQ 300
           FTEAQIKC+VQQLLHGLEHCHSRG+LHRDIKGSNLL+D+NGVLKIGDFGLATFF+P+QKQ
Sbjct: 241 FTEAQIKCYVQQLLHGLEHCHSRGILHRDIKGSNLLLDNNGVLKIGDFGLATFFQPDQKQ 300

Query: 301 PLTSRVVTLWYRAPELLLGATDYGPAIDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK 360
           PLTSRVVTLWYRAPELLLGAT YGP IDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK
Sbjct: 301 PLTSRVVTLWYRAPELLLGATQYGPGIDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK 360

Query: 361 LCGSPSEEFWRRTKLPHATSFKPHHPYKSCLSETFKNFPSSALALVNKLLAIEPEHRGSA 420
           LCGSPSEEFWRRTKLPHATSFKP   YKSCLSETFK+FP SALALVNKLLAIEPEHRGSA
Sbjct: 361 LCGSPSEEFWRRTKLPHATSFKPQSRYKSCLSETFKSFPPSALALVNKLLAIEPEHRGSA 420

Query: 421 TSALSSEFFITEPLPCDPSSLPKYPPSKEYDAKLRNEEERK 462
           T AL SEFF TEPLPCDPSSLPKYPPSKE+DAKLRNEEERK
Sbjct: 421 TLALRSEFFRTEPLPCDPSSLPKYPPSKEFDAKLRNEEERK 461

BLAST of MS006336 vs. ExPASy Swiss-Prot
Match: F4I114 (Probable serine/threonine-protein kinase At1g09600 OS=Arabidopsis thaliana OX=3702 GN=At1g09600 PE=3 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 3.3e-160
Identity = 298/483 (61.70%), Postives = 355/483 (73.50%), Query Frame = 0

Query: 1   MGCICSRSSSEPEDSNQKKSEKDLNKSSVQLVAPSPSKGEDLLLEIQLSRQASKVKGGSV 60
           MGC C++  + P++ N   S   ++  +V+     P K      + + S+ AS  K  +V
Sbjct: 1   MGCNCTK-GTRPDNDNVDNSNSIVSNVNVKERRSKPKKTPK---KKKKSKSASSSKDNNV 60

Query: 61  -HKTKANGGSHH--------HRAKDDEAENK---SVKAGKANNDNASTG----------G 120
             + ++N                KDDE+E K     K+ +       TG           
Sbjct: 61  GFEERSNDNKEASLTLLIPIDAKKDDESEKKVNLERKSSRLVFQRRPTGIEVGANNIGTL 120

Query: 121 KQPEVFRLASLSKAAETELVMAGWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSS 180
           +QP++ R+ S+S       VMAGWPSWLASVAGEAI GW+PR+ADSFEKL+KIGQGTYSS
Sbjct: 121 QQPKMTRICSVSNGERGAQVMAGWPSWLASVAGEAINGWIPRKADSFEKLEKIGQGTYSS 180

Query: 181 VYKARDLETGKTVALKKVRFVNMDPESVRFMAREIYILRKLDHPNVMKLECLVTSRVSGS 240
           VYKARDLET + VALKKVRF NMDP+SVRFMAREI ILR+LDHPNVMKLE L+TSRVSGS
Sbjct: 181 VYKARDLETNQLVALKKVRFANMDPDSVRFMAREIIILRRLDHPNVMKLEGLITSRVSGS 240

Query: 241 LYLVFEYMEHDLAGLAAAPGHKFTEAQIKCFVQQLLHGLEHCHSRGVLHRDIKGSNLLVD 300
           +YL+FEYMEHDLAGLA+ PG  F+EAQIKC+++QLLHGLEHCHSRGVLHRDIKGSNLL+D
Sbjct: 241 MYLIFEYMEHDLAGLASTPGINFSEAQIKCYMKQLLHGLEHCHSRGVLHRDIKGSNLLLD 300

Query: 301 SNGVLKIGDFGLATFFKPEQKQPLTSRVVTLWYRAPELLLGATDYGPAIDLWSCGCIVAE 360
            N  LKIGDFGLA F++  QKQPLTSRVVTLWYR PELLLG+TDYG  +DLWS GCI+AE
Sbjct: 301 HNNNLKIGDFGLANFYQGHQKQPLTSRVVTLWYRPPELLLGSTDYGVTVDLWSTGCILAE 360

Query: 361 LFAGKPIMPGRTEVEQMHKIFKLCGSPSEEFWRRTKLPHATSFKPHHPYKSCLSETFKNF 420
           LF GKPIMPGRTEVEQ+HKIFKLCGSPSEE+W+ +KLPHAT FKP  PYK C++ETFK+ 
Sbjct: 361 LFTGKPIMPGRTEVEQLHKIFKLCGSPSEEYWKISKLPHATIFKPQQPYKRCVAETFKSL 420

Query: 421 PSSALALVNKLLAIEPEHRGSATSALSSEFFITEPLPCDPSSLPKYPPSKEYDAKLRNEE 462
           PSSALALV  LLA+EP+ RG+  SAL SEFF T PL  DPSSLPKY P KE D K + EE
Sbjct: 421 PSSALALVEVLLAVEPDARGTTASALESEFFTTSPLASDPSSLPKYQPRKEIDVKAQEEE 479

BLAST of MS006336 vs. ExPASy Swiss-Prot
Match: Q9ZVM9 (Probable serine/threonine-protein kinase At1g54610 OS=Arabidopsis thaliana OX=3702 GN=At1g54610 PE=1 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 5.0e-148
Identity = 258/406 (63.55%), Postives = 319/406 (78.57%), Query Frame = 0

Query: 58  GSVHKTKANG--GSHHHRAKDDEAENKSVKAGKANNDNASTGGKQPEVFRLASLSKAAET 117
           G    TK+NG       + K++EA     +     +   ST   +P   RL++ SK    
Sbjct: 33  GESSVTKSNGVIADDVEKKKNEEANGDKERKSSKGDRRRST---KPNP-RLSNPSKHWRG 92

Query: 118 ELVMAGWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGKTVALKK 177
           E V AGWPSWL+   GEA+ GWVPR+AD+FEK+DKIGQGTYS+VYKA+D+ TGK VALKK
Sbjct: 93  EQVAAGWPSWLSDACGEALNGWVPRKADTFEKIDKIGQGTYSNVYKAKDMLTGKIVALKK 152

Query: 178 VRFVNMDPESVRFMAREIYILRKLDHPNVMKLECLVTSRVSGSLYLVFEYMEHDLAGLAA 237
           VRF N++PESV+FMAREI +LR+LDHPNV+KLE LVTSR+S SLYLVF+YM+HDLAGLA+
Sbjct: 153 VRFDNLEPESVKFMAREILVLRRLDHPNVVKLEGLVTSRMSCSLYLVFQYMDHDLAGLAS 212

Query: 238 APGHKFTEAQIKCFVQQLLHGLEHCHSRGVLHRDIKGSNLLVDSNGVLKIGDFGLATFFK 297
           +P  KF+E+++KC ++QL+ GLEHCHSRGVLHRDIKGSNLL+D  GVLKI DFGLAT F 
Sbjct: 213 SPVVKFSESEVKCLMRQLISGLEHCHSRGVLHRDIKGSNLLIDDGGVLKIADFGLATIFD 272

Query: 298 PEQKQPLTSRVVTLWYRAPELLLGATDYGPAIDLWSCGCIVAELFAGKPIMPGRTEVEQM 357
           P  K+P+TSRVVTLWYRAPELLLGATDYG  IDLWS GCI+AEL AG+PIMPGRTEVEQ+
Sbjct: 273 PNHKRPMTSRVVTLWYRAPELLLGATDYGVGIDLWSAGCILAELLAGRPIMPGRTEVEQL 332

Query: 358 HKIFKLCGSPSEEFWRRTKLPHATSFKPHHPYKSCLSETFKNFPSSALALVNKLLAIEPE 417
           HKI+KLCGSPSE++W++ K  H   +KP  PYK  + ETFK+FP S+L L++ LL+IEPE
Sbjct: 333 HKIYKLCGSPSEDYWKKGKFTHGAIYKPREPYKRSIRETFKDFPPSSLPLIDALLSIEPE 392

Query: 418 HRGSATSALSSEFFITEPLPCDPSSLPKYPPSKEYDAKLRNEEERK 462
            R +A++AL SEFF +EP  C+P+ LPKYPPSKE DAK R+EE R+
Sbjct: 393 DRQTASAALKSEFFTSEPYACEPADLPKYPPSKEIDAKRRDEETRR 434

BLAST of MS006336 vs. ExPASy Swiss-Prot
Match: F4ICB6 (Protein IMPAIRED IN BABA-INDUCED STERILITY 1 OS=Arabidopsis thaliana OX=3702 GN=IBS1 PE=3 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 1.5e-136
Identity = 232/361 (64.27%), Postives = 289/361 (80.06%), Query Frame = 0

Query: 104 FRLASLSKAAETELVMAGWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKAR 163
           FRL +LSK  E E V AGWP+WL++VAGEAI GWVP R+D+FEKL+KIGQGTYSSV++AR
Sbjct: 90  FRLGNLSKYLEAEQVAAGWPAWLSNVAGEAIHGWVPFRSDAFEKLEKIGQGTYSSVFRAR 149

Query: 164 DLETGKTVALKKVRFVNMDPESVRFMAREIYILRKLDHPNVMKLECLVTSRVSGSLYLVF 223
           + ETG+ VALKKVRF N +PESVRFMAREI ILRKL+HPN++KLE +VTS++S S++LVF
Sbjct: 150 ETETGRIVALKKVRFDNFEPESVRFMAREILILRKLNHPNIIKLEGIVTSKLSCSIHLVF 209

Query: 224 EYMEHDLAGLAAAPGHKFTEAQIKCFVQQLLHGLEHCHSRGVLHRDIKGSNLLVDSNGVL 283
           EYMEHDL GL ++P   FT  QIKC+++QLL GL+HCH+RGV+HRDIKGSNLLV++ G+L
Sbjct: 210 EYMEHDLTGLLSSPDIDFTTPQIKCYMKQLLSGLDHCHARGVMHRDIKGSNLLVNNEGIL 269

Query: 284 KIGDFGLATFFKPE-QKQPLTSRVVTLWYRAPELLLGATDYGPAIDLWSCGCIVAELFAG 343
           K+ DFGLA F      KQPLTSRVVTLWYR PELLLGAT+YG ++DLWS GC+ AEL  G
Sbjct: 270 KVADFGLANFCNASGNKQPLTSRVVTLWYRPPELLLGATEYGASVDLWSVGCVFAELLIG 329

Query: 344 KPIMPGRTEVEQMHKIFKLCGSPSEEFWRRTKLPHATSFKPHHPYKSCLSET--FKNFPS 403
           KP++ GRTEVEQ+HKIFKLCGSP E++W+++KLPHA  FKP   Y  CL ET   K    
Sbjct: 330 KPVLQGRTEVEQLHKIFKLCGSPPEDYWKKSKLPHAMLFKPQQHYDGCLRETLKLKGLSD 389

Query: 404 SALALVNKLLAIEPEHRGSATSALSSEFFITEPLPCDPSSLPKYPPSKEYDAKLRNEEER 462
           + + L+  LL+I+P  RG+A++AL S++F ++P  CDPSSLP Y PSKE DAK R +  R
Sbjct: 390 ADINLIETLLSIQPHKRGTASTALVSQYFTSKPFACDPSSLPVYSPSKEIDAKHREDTTR 449

BLAST of MS006336 vs. ExPASy Swiss-Prot
Match: Q5JK68 (Cyclin-dependent kinase C-2 OS=Oryza sativa subsp. japonica OX=39947 GN=CDKC-2 PE=2 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 2.6e-96
Identity = 176/341 (51.61%), Postives = 229/341 (67.16%), Query Frame = 0

Query: 137 WVPRRADSFEKLDKIGQGTYSSVYKARDLETGKTVALKKVRFVNMDPESVRFMAREIYIL 196
           W  R  D FEKL++IG+GTY  VY A++ ET + VALKK+R  N          REI IL
Sbjct: 17  WGSRSVDCFEKLEQIGEGTYGQVYMAKETETNEIVALKKIRMDNEREGFPITAIREIKIL 76

Query: 197 RKLDHPNVMKLECLVTS---------------RVSGSLYLVFEYMEHDLAGLAAAPGHKF 256
           +KL H NV++L+ +VTS               +  GS+Y+VFEYM+HDL GLA  PG +F
Sbjct: 77  KKLHHQNVIQLKEIVTSPGPERDEQGKPIEGNKYKGSIYMVFEYMDHDLTGLADRPGMRF 136

Query: 257 TEAQIKCFVQQLLHGLEHCHSRGVLHRDIKGSNLLVDSNGVLKIGDFGLATFFKPEQKQP 316
           T  QIKC+++QLL GL +CH   VLHRDIKGSNLL+D+ G LK+ DFGLA  F  +    
Sbjct: 137 TVPQIKCYMRQLLTGLHYCHVNQVLHRDIKGSNLLIDNEGNLKLADFGLARSFSSDHNGN 196

Query: 317 LTSRVVTLWYRAPELLLGATDYGPAIDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFKL 376
           LT+RV+TLWYR PELLLG+T YGPA+D+WS GCI AEL  GKPI+ G+ E EQ+ KIF+L
Sbjct: 197 LTNRVITLWYRPPELLLGSTRYGPAVDMWSVGCIFAELLNGKPILTGKNEPEQLSKIFEL 256

Query: 377 CGSPSEEFWRR-TKLPHATSFKPHHPYKSCLSETFKNFPSSALALVNKLLAIEPEHRGSA 436
           CG+P E  W   TK+P   +FKP  P K  + E+FK+F   AL L+ K+L ++P  R SA
Sbjct: 257 CGTPDELIWPGVTKMPWYNNFKPQRPMKRRVKESFKHFDQHALDLLEKMLTLDPSQRISA 316

Query: 437 TSALSSEFFITEPLPCDPSSLPKYPPSKEYDAKLRNEEERK 462
             AL +E+F T+PLPCDP SLPKY  S E+  K + +++R+
Sbjct: 317 KDALDAEYFWTDPLPCDPKSLPKYEASHEFQTKKKRQQQRQ 357

BLAST of MS006336 vs. ExPASy Swiss-Prot
Match: Q9LFT8 (Cyclin-dependent kinase C-1 OS=Arabidopsis thaliana OX=3702 GN=CDKC-1 PE=1 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 2.2e-95
Identity = 174/340 (51.18%), Postives = 230/340 (67.65%), Query Frame = 0

Query: 137 WVPRRADSFEKLDKIGQGTYSSVYKARDLETGKTVALKKVRFVNMDPESVRFMAREIYIL 196
           W  R  D FEKL++IG+GTY  VY A++++TG+ VALKK+R  N          REI IL
Sbjct: 18  WGSRSVDCFEKLEQIGEGTYGQVYMAKEIKTGEIVALKKIRMDNEREGFPITAIREIKIL 77

Query: 197 RKLDHPNVMKLECLVTS--------------RVSGSLYLVFEYMEHDLAGLAAAPGHKFT 256
           +KL H NV++L+ +VTS              +  G +Y+VFEYM+HDL GLA  PG +FT
Sbjct: 78  KKLHHENVIQLKEIVTSPGRDRDDQGKPDNNKYKGGIYMVFEYMDHDLTGLADRPGLRFT 137

Query: 257 EAQIKCFVQQLLHGLEHCHSRGVLHRDIKGSNLLVDSNGVLKIGDFGLATFFKPEQKQPL 316
             QIKC+++QLL GL +CH   VLHRDIKGSNLL+D+ G LK+ DFGLA  +  +    L
Sbjct: 138 VPQIKCYMKQLLTGLHYCHVNQVLHRDIKGSNLLIDNEGNLKLADFGLARSYSHDHTGNL 197

Query: 317 TSRVVTLWYRAPELLLGATDYGPAIDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFKLC 376
           T+RV+TLWYR PELLLGAT YGPAID+WS GCI AEL   KPI+PG+ E EQ++KIF+LC
Sbjct: 198 TNRVITLWYRPPELLLGATKYGPAIDMWSVGCIFAELLHAKPILPGKNEQEQLNKIFELC 257

Query: 377 GSPSEEFWRR-TKLPHATSFKPHHPYKSCLSETFKNFPSSALALVNKLLAIEPEHRGSAT 436
           GSP E+ W   +K+P   +FKP  P K  + E F++F   AL L+ K+L ++P  R SA 
Sbjct: 258 GSPDEKLWPGVSKMPWFNNFKPARPLKRRVREFFRHFDRHALELLEKMLVLDPAQRISAK 317

Query: 437 SALSSEFFITEPLPCDPSSLPKYPPSKEYDAKLRNEEERK 462
            AL +E+F T+PLPCDP SLP Y  S E+  K + +++R+
Sbjct: 318 DALDAEYFWTDPLPCDPKSLPTYESSHEFQTKKKRQQQRQ 357

BLAST of MS006336 vs. ExPASy TrEMBL
Match: A0A6J1CK27 (probable serine/threonine-protein kinase At1g09600 OS=Momordica charantia OX=3673 GN=LOC111012150 PE=4 SV=1)

HSP 1 Score: 922.9 bits (2384), Expect = 5.2e-265
Identity = 461/461 (100.00%), Postives = 461/461 (100.00%), Query Frame = 0

Query: 1   MGCICSRSSSEPEDSNQKKSEKDLNKSSVQLVAPSPSKGEDLLLEIQLSRQASKVKGGSV 60
           MGCICSRSSSEPEDSNQKKSEKDLNKSSVQLVAPSPSKGEDLLLEIQLSRQASKVKGGSV
Sbjct: 1   MGCICSRSSSEPEDSNQKKSEKDLNKSSVQLVAPSPSKGEDLLLEIQLSRQASKVKGGSV 60

Query: 61  HKTKANGGSHHHRAKDDEAENKSVKAGKANNDNASTGGKQPEVFRLASLSKAAETELVMA 120
           HKTKANGGSHHHRAKDDEAENKSVKAGKANNDNASTGGKQPEVFRLASLSKAAETELVMA
Sbjct: 61  HKTKANGGSHHHRAKDDEAENKSVKAGKANNDNASTGGKQPEVFRLASLSKAAETELVMA 120

Query: 121 GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGKTVALKKVRFVN 180
           GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGKTVALKKVRFVN
Sbjct: 121 GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGKTVALKKVRFVN 180

Query: 181 MDPESVRFMAREIYILRKLDHPNVMKLECLVTSRVSGSLYLVFEYMEHDLAGLAAAPGHK 240
           MDPESVRFMAREIYILRKLDHPNVMKLECLVTSRVSGSLYLVFEYMEHDLAGLAAAPGHK
Sbjct: 181 MDPESVRFMAREIYILRKLDHPNVMKLECLVTSRVSGSLYLVFEYMEHDLAGLAAAPGHK 240

Query: 241 FTEAQIKCFVQQLLHGLEHCHSRGVLHRDIKGSNLLVDSNGVLKIGDFGLATFFKPEQKQ 300
           FTEAQIKCFVQQLLHGLEHCHSRGVLHRDIKGSNLLVDSNGVLKIGDFGLATFFKPEQKQ
Sbjct: 241 FTEAQIKCFVQQLLHGLEHCHSRGVLHRDIKGSNLLVDSNGVLKIGDFGLATFFKPEQKQ 300

Query: 301 PLTSRVVTLWYRAPELLLGATDYGPAIDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK 360
           PLTSRVVTLWYRAPELLLGATDYGPAIDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK
Sbjct: 301 PLTSRVVTLWYRAPELLLGATDYGPAIDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK 360

Query: 361 LCGSPSEEFWRRTKLPHATSFKPHHPYKSCLSETFKNFPSSALALVNKLLAIEPEHRGSA 420
           LCGSPSEEFWRRTKLPHATSFKPHHPYKSCLSETFKNFPSSALALVNKLLAIEPEHRGSA
Sbjct: 361 LCGSPSEEFWRRTKLPHATSFKPHHPYKSCLSETFKNFPSSALALVNKLLAIEPEHRGSA 420

Query: 421 TSALSSEFFITEPLPCDPSSLPKYPPSKEYDAKLRNEEERK 462
           TSALSSEFFITEPLPCDPSSLPKYPPSKEYDAKLRNEEERK
Sbjct: 421 TSALSSEFFITEPLPCDPSSLPKYPPSKEYDAKLRNEEERK 461

BLAST of MS006336 vs. ExPASy TrEMBL
Match: A0A0A0KSU9 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G650500 PE=4 SV=1)

HSP 1 Score: 842.0 bits (2174), Expect = 1.2e-240
Identity = 418/461 (90.67%), Postives = 435/461 (94.36%), Query Frame = 0

Query: 1   MGCICSRSSSEPEDSNQKKSEKDLNKSSVQLVAPSPSKGEDLLLEIQLSRQASKVKGGSV 60
           MGCICSR SSEPEDS+ K+ EK+LNKSSVQLVAPSPSK ED+LLEIQL+RQASK  GGSV
Sbjct: 1   MGCICSRDSSEPEDSSHKEPEKELNKSSVQLVAPSPSKREDMLLEIQLTRQASKANGGSV 60

Query: 61  HKTKANGGSHHHRAKDDEAENKSVKAGKANNDNASTGGKQPEVFRLASLSKAAETELVMA 120
           HK K NGGS H   +DDE+ENKSVKA KANNDNAS GGKQP++FRLASLSK AETELVMA
Sbjct: 61  HKAKVNGGSVHRAKEDDESENKSVKAAKANNDNASVGGKQPDIFRLASLSKGAETELVMA 120

Query: 121 GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGKTVALKKVRFVN 180
           GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGK VALKKVRFVN
Sbjct: 121 GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGKIVALKKVRFVN 180

Query: 181 MDPESVRFMAREIYILRKLDHPNVMKLECLVTSRVSGSLYLVFEYMEHDLAGLAAAPGHK 240
           MDPESVRFMAREIYILRKLDHPNVMKLE LVTSR+SGSLYLVFEYMEHDLAGLAA PGHK
Sbjct: 181 MDPESVRFMAREIYILRKLDHPNVMKLESLVTSRMSGSLYLVFEYMEHDLAGLAAVPGHK 240

Query: 241 FTEAQIKCFVQQLLHGLEHCHSRGVLHRDIKGSNLLVDSNGVLKIGDFGLATFFKPEQKQ 300
           FTEAQIKC+VQQLLHGLEHCHSRG+LHRDIKGSNLLVD+NGVLKIGDFGLATFF+P+QKQ
Sbjct: 241 FTEAQIKCYVQQLLHGLEHCHSRGILHRDIKGSNLLVDNNGVLKIGDFGLATFFQPDQKQ 300

Query: 301 PLTSRVVTLWYRAPELLLGATDYGPAIDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK 360
           PLTSRVVTLWYRAPELLLGAT YGP IDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK
Sbjct: 301 PLTSRVVTLWYRAPELLLGATQYGPGIDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK 360

Query: 361 LCGSPSEEFWRRTKLPHATSFKPHHPYKSCLSETFKNFPSSALALVNKLLAIEPEHRGSA 420
           LCGSPSEEFWRRTKLPHATSFKP H YKSCLSETFK+FP SALALVNKLLAIEPEHRGSA
Sbjct: 361 LCGSPSEEFWRRTKLPHATSFKPQHRYKSCLSETFKSFPPSALALVNKLLAIEPEHRGSA 420

Query: 421 TSALSSEFFITEPLPCDPSSLPKYPPSKEYDAKLRNEEERK 462
           T AL SEFF TEPLPCDPSSLPKYPPSKE+DAKLRNEEERK
Sbjct: 421 TLALRSEFFRTEPLPCDPSSLPKYPPSKEFDAKLRNEEERK 461

BLAST of MS006336 vs. ExPASy TrEMBL
Match: A0A5D3BMF1 (Putative serine/threonine-protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00480 PE=4 SV=1)

HSP 1 Score: 839.3 bits (2167), Expect = 7.5e-240
Identity = 417/461 (90.46%), Postives = 436/461 (94.58%), Query Frame = 0

Query: 1   MGCICSRSSSEPEDSNQKKSEKDLNKSSVQLVAPSPSKGEDLLLEIQLSRQASKVKGGSV 60
           MGCICSR SSEPEDS+ K+ EK+LNKSSVQLVAPSPSK ED+LLEIQLSRQASK  GGSV
Sbjct: 1   MGCICSRDSSEPEDSSHKEPEKELNKSSVQLVAPSPSKREDMLLEIQLSRQASKANGGSV 60

Query: 61  HKTKANGGSHHHRAKDDEAENKSVKAGKANNDNASTGGKQPEVFRLASLSKAAETELVMA 120
           HK KANGGS H   +DDE+ENKS+KA KANNDNAS GGKQP++FRLASLSK AETELVMA
Sbjct: 61  HKAKANGGSVHRAKEDDESENKSLKAAKANNDNASVGGKQPDIFRLASLSKGAETELVMA 120

Query: 121 GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGKTVALKKVRFVN 180
           GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGK VALKKVRFVN
Sbjct: 121 GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGKIVALKKVRFVN 180

Query: 181 MDPESVRFMAREIYILRKLDHPNVMKLECLVTSRVSGSLYLVFEYMEHDLAGLAAAPGHK 240
           MDPESVRFMAREIYILRKLDHPNVMKLE LVTSR+SGSLYLVFEYMEHDLAGL+AAPGHK
Sbjct: 181 MDPESVRFMAREIYILRKLDHPNVMKLESLVTSRMSGSLYLVFEYMEHDLAGLSAAPGHK 240

Query: 241 FTEAQIKCFVQQLLHGLEHCHSRGVLHRDIKGSNLLVDSNGVLKIGDFGLATFFKPEQKQ 300
           FTEAQIKC+VQQLLHGLEHCHSRG+LHRDIKGSNLL+D+NGVLKIGDFGLATFF+P+QKQ
Sbjct: 241 FTEAQIKCYVQQLLHGLEHCHSRGILHRDIKGSNLLLDNNGVLKIGDFGLATFFQPDQKQ 300

Query: 301 PLTSRVVTLWYRAPELLLGATDYGPAIDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK 360
           PLTSRVVTLWYRAPELLLGAT YGP IDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK
Sbjct: 301 PLTSRVVTLWYRAPELLLGATQYGPGIDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK 360

Query: 361 LCGSPSEEFWRRTKLPHATSFKPHHPYKSCLSETFKNFPSSALALVNKLLAIEPEHRGSA 420
           LCGSPSEEFWRRTKLPHATSFKP   YKSCLSETFK+FP SALALVNKLLAIEPEHRGSA
Sbjct: 361 LCGSPSEEFWRRTKLPHATSFKPQSRYKSCLSETFKSFPPSALALVNKLLAIEPEHRGSA 420

Query: 421 TSALSSEFFITEPLPCDPSSLPKYPPSKEYDAKLRNEEERK 462
           T AL SEFF TEPLPCDPSSLPKYPPSKE+DAKLRNEEERK
Sbjct: 421 TLALRSEFFRTEPLPCDPSSLPKYPPSKEFDAKLRNEEERK 461

BLAST of MS006336 vs. ExPASy TrEMBL
Match: A0A1S3CAR0 (probable serine/threonine-protein kinase At1g09600 OS=Cucumis melo OX=3656 GN=LOC103498696 PE=4 SV=1)

HSP 1 Score: 833.9 bits (2153), Expect = 3.2e-238
Identity = 415/461 (90.02%), Postives = 434/461 (94.14%), Query Frame = 0

Query: 1   MGCICSRSSSEPEDSNQKKSEKDLNKSSVQLVAPSPSKGEDLLLEIQLSRQASKVKGGSV 60
           MGCICSR SSEPEDS+ K+ EK+ NKSSVQLVAPSPSK ED+LLEIQLSRQASK  GGSV
Sbjct: 1   MGCICSRDSSEPEDSSHKEPEKEFNKSSVQLVAPSPSKREDMLLEIQLSRQASKANGGSV 60

Query: 61  HKTKANGGSHHHRAKDDEAENKSVKAGKANNDNASTGGKQPEVFRLASLSKAAETELVMA 120
           HK KANGGS H   +DDE+ENKS+KA KANNDNAS GGKQ ++FRLASLSK AETELVMA
Sbjct: 61  HKAKANGGSVHRAKEDDESENKSLKAAKANNDNASVGGKQLDIFRLASLSKGAETELVMA 120

Query: 121 GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGKTVALKKVRFVN 180
           GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGK VALKKVRFVN
Sbjct: 121 GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGKIVALKKVRFVN 180

Query: 181 MDPESVRFMAREIYILRKLDHPNVMKLECLVTSRVSGSLYLVFEYMEHDLAGLAAAPGHK 240
           MDPESVRFMAREIYILRKLDHPNVMKLE LVTSR+SGSLYLVFEYMEHDLAGL+AAPGHK
Sbjct: 181 MDPESVRFMAREIYILRKLDHPNVMKLESLVTSRMSGSLYLVFEYMEHDLAGLSAAPGHK 240

Query: 241 FTEAQIKCFVQQLLHGLEHCHSRGVLHRDIKGSNLLVDSNGVLKIGDFGLATFFKPEQKQ 300
           FTEAQIKC+VQQLLHGLEHCHSRG+LHRDIKGSNLL+D+NGVLKIGDFGLATFF+P+QKQ
Sbjct: 241 FTEAQIKCYVQQLLHGLEHCHSRGILHRDIKGSNLLLDNNGVLKIGDFGLATFFQPDQKQ 300

Query: 301 PLTSRVVTLWYRAPELLLGATDYGPAIDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK 360
           PLTSRVVTLWYRAPELLLGAT YGP IDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK
Sbjct: 301 PLTSRVVTLWYRAPELLLGATQYGPGIDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK 360

Query: 361 LCGSPSEEFWRRTKLPHATSFKPHHPYKSCLSETFKNFPSSALALVNKLLAIEPEHRGSA 420
           LCGSPSEEFWRRTKLPHATSFKP   YKSCLSETFK+FP SALALVNKLLAIEPEHRGSA
Sbjct: 361 LCGSPSEEFWRRTKLPHATSFKPQSRYKSCLSETFKSFPPSALALVNKLLAIEPEHRGSA 420

Query: 421 TSALSSEFFITEPLPCDPSSLPKYPPSKEYDAKLRNEEERK 462
           T AL SEFF TEPLPCDPSSLPKYPPSKE+DAKLRNEEERK
Sbjct: 421 TLALRSEFFRTEPLPCDPSSLPKYPPSKEFDAKLRNEEERK 461

BLAST of MS006336 vs. ExPASy TrEMBL
Match: A0A6J1GBD6 (probable serine/threonine-protein kinase At1g54610 OS=Cucurbita moschata OX=3662 GN=LOC111452616 PE=4 SV=1)

HSP 1 Score: 782.7 bits (2020), Expect = 8.3e-223
Identity = 394/472 (83.47%), Postives = 425/472 (90.04%), Query Frame = 0

Query: 1   MGCICSRSSSEPEDSNQKKSE--KDLNKSSVQLVAPSPSKGEDLLLEIQLSRQASKVKGG 60
           MGCICSR SS PEDS+QK+S   KD+NKSSVQLVAPSPSKGEDLL EI+LSRQASK  GG
Sbjct: 1   MGCICSRDSSGPEDSSQKESRTGKDVNKSSVQLVAPSPSKGEDLLSEIKLSRQASKANGG 60

Query: 61  SVHKTKANGGSHH---------HRAKDDEAENKSVKAGKANNDNASTGGKQPEVFRLASL 120
           SVH+TKANGGS H         HRA++D+   +S+K  KA  DN S  G++P++FRLASL
Sbjct: 61  SVHQTKANGGSVHKTKANGGSVHRAREDD---ESIKPAKAKADNTSAAGRKPDIFRLASL 120

Query: 121 SKAAETELVMAGWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGK 180
           SK AE E VMAGWPSWLASVAGEAIKGWVPRR DSFEKL+KIGQGTYSSVYKARDLETGK
Sbjct: 121 SKVAEAEYVMAGWPSWLASVAGEAIKGWVPRRIDSFEKLEKIGQGTYSSVYKARDLETGK 180

Query: 181 TVALKKVRFVNMDPESVRFMAREIYILRKLDHPNVMKLECLVTSRVSGSLYLVFEYMEHD 240
           TVALKKVRFVNMD ESVRFMAREIYILRKLDHPNVMKLE LVTS++SGSL+LVFEYM+HD
Sbjct: 181 TVALKKVRFVNMDRESVRFMAREIYILRKLDHPNVMKLEGLVTSKMSGSLHLVFEYMDHD 240

Query: 241 LAGLAAAPGHKFTEAQIKCFVQQLLHGLEHCHSRGVLHRDIKGSNLLVDSNGVLKIGDFG 300
           LAGLAAAPGHKF+EAQIKC+VQQL+ GLEHCHSRG+LHRDIKGSNLL+D NGVLKIGDFG
Sbjct: 241 LAGLAAAPGHKFSEAQIKCYVQQLMQGLEHCHSRGILHRDIKGSNLLIDDNGVLKIGDFG 300

Query: 301 LATFFKPEQKQPLTSRVVTLWYRAPELLLGATDYGPAIDLWSCGCIVAELFAGKPIMPGR 360
           LAT F+P+QKQPLTSRVVTLWYRAPELLLGAT+YGPAIDLWSCGCIVAELFAGKPIMPGR
Sbjct: 301 LATCFQPDQKQPLTSRVVTLWYRAPELLLGATEYGPAIDLWSCGCIVAELFAGKPIMPGR 360

Query: 361 TEVEQMHKIFKLCGSPSEEFWRRTKLPHATSFKPHHPYKSCLSETFKNFPSSALALVNKL 420
           TEVEQMHKIFKLCGSPSEEFWRRTKLP +TSFKP H YKSC+SETFK+FP  ALALVNKL
Sbjct: 361 TEVEQMHKIFKLCGSPSEEFWRRTKLPLSTSFKPQHRYKSCISETFKSFPPPALALVNKL 420

Query: 421 LAIEPEHRGSATSALSSEFFITEPLPCDPSSLPKYPPSKEYDAKLRNEEERK 462
           LAIEPEHRGSA   L SEFF TEPLPCDPSSLPKYPPSKE+DAKLRNEEERK
Sbjct: 421 LAIEPEHRGSAAEVLRSEFFRTEPLPCDPSSLPKYPPSKEFDAKLRNEEERK 469

BLAST of MS006336 vs. TAIR 10
Match: AT1G33770.1 (Protein kinase superfamily protein )

HSP 1 Score: 605.5 bits (1560), Expect = 3.5e-173
Identity = 319/471 (67.73%), Postives = 375/471 (79.62%), Query Frame = 0

Query: 1   MGCICSRSSSEPEDS---NQKKSEKDLNK-SSVQLVAPSPSKGEDLLLEIQLSRQASKVK 60
           MGCICS+ ++E E+    +++K+ +  NK SSVQL+AP PS  +D       S +A  V 
Sbjct: 1   MGCICSKGAAEDEEGVVYHREKANEYWNKSSSVQLIAPLPSNKDD------FSHKA--VD 60

Query: 61  GGSVHKTKANG------GSHHHRAKDDEAENKSVKAGKANNDNASTGGKQPEVFRLASLS 120
           G S    +A+G       SH  +    E  ++S + G+  +DN   GG       ++++ 
Sbjct: 61  GSSGGGRRASGLIVPIDDSHDGKTVIVERPSRS-QRGRRVSDNGKGGG-----LIISNVP 120

Query: 121 KAAETELVMAGWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGKT 180
           ++AE EL+ AGWP WL SVAGEAIKGWVPRRADSFEKLDKIGQGTYS VYKARDLETGK 
Sbjct: 121 RSAEAELIAAGWPYWLTSVAGEAIKGWVPRRADSFEKLDKIGQGTYSIVYKARDLETGKI 180

Query: 181 VALKKVRFVNMDPESVRFMAREIYILRKLDHPNVMKLECLVTSRVSGSLYLVFEYMEHDL 240
           VA+KKVRF NMDPESVRFMAREI ILRKLDHPNVMKL+CLVTS++SGSL+LVFEYMEHDL
Sbjct: 181 VAMKKVRFANMDPESVRFMAREINILRKLDHPNVMKLQCLVTSKLSGSLHLVFEYMEHDL 240

Query: 241 AGLAAAPGHKFTEAQIKCFVQQLLHGLEHCHSRGVLHRDIKGSNLLVDSNGVLKIGDFGL 300
           +GLA  PG KFTE QIKCF++QLL GLEHCHSRG+LHRDIKGSNLLV+++GVLKIGDFGL
Sbjct: 241 SGLALRPGVKFTEPQIKCFMKQLLCGLEHCHSRGILHRDIKGSNLLVNNDGVLKIGDFGL 300

Query: 301 ATFFKPEQKQPLTSRVVTLWYRAPELLLGATDYGPAIDLWSCGCIVAELFAGKPIMPGRT 360
           A+F+KP+Q QPLTSRVVTLWYRAPELLLG+T+YGPAIDLWS GCI+AELF  KPIMPGRT
Sbjct: 301 ASFYKPDQDQPLTSRVVTLWYRAPELLLGSTEYGPAIDLWSVGCILAELFVCKPIMPGRT 360

Query: 361 EVEQMHKIFKLCGSPSEEFWRRTKLPHATSFKPHHPYKSCLSETFKNFPSSALALVNKLL 420
           EVEQMHKIFKLCGSPSEEFW  TK P ATS+KP HPYK  L ETFKN  SS+L L++KLL
Sbjct: 361 EVEQMHKIFKLCGSPSEEFWNTTKFPQATSYKPQHPYKRVLLETFKNLSSSSLDLLDKLL 420

Query: 421 AIEPEHRGSATSALSSEFFITEPLPCDPSSLPKYPPSKEYDAKLRNEEERK 462
           ++EPE R SA+S L SEFF TEPLPC  SSLPKYPPSKE DAK+R+EE ++
Sbjct: 421 SVEPEKRCSASSTLLSEFFTTEPLPCHISSLPKYPPSKELDAKVRDEEAKR 457

BLAST of MS006336 vs. TAIR 10
Match: AT1G71530.1 (Protein kinase superfamily protein )

HSP 1 Score: 582.0 bits (1499), Expect = 4.2e-166
Identity = 317/474 (66.88%), Postives = 351/474 (74.05%), Query Frame = 0

Query: 1   MGCICSRSSSEPEDSNQKKSEKDLNKSSVQLVAPSPSKGEDLLLEIQLSRQASKVKGGSV 60
           MGCIC+ + S P  +   K   D +KS +QL+   P            S  +SK +G   
Sbjct: 1   MGCICATARS-PSAAVTDKDLLDSSKSILQLLPHHPPS----------SSSSSKKEGSFT 60

Query: 61  HKTK-------ANGGSHHHR--AKDDEAENKSVKAGKANNDNASTGGKQPEVFRLASLSK 120
             T        ANG     R     D    K V    A   N +       V + A    
Sbjct: 61  RTTSSASITIVANGYPVARRPSTSSDRNSTKPVVVVGAPTRNPTRRVTAIPVAQPAQQQP 120

Query: 121 AA----ETELVMAGWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLET 180
           A     +TEL  A WPSWLASVAGEAIKGWVPR A+SFEKLDKIGQGTYSSVYKARDLET
Sbjct: 121 ARVISNKTELPAAEWPSWLASVAGEAIKGWVPRCAESFEKLDKIGQGTYSSVYKARDLET 180

Query: 181 GKTVALKKVRFVNMDPESVRFMAREIYILRKLDHPNVMKLECLVTSRVSGSLYLVFEYME 240
           GK VA+KKVRFVNMDPESVRFMAREI ILRKLDHPNVMKLE LVTSR+SGSLYLVFEYME
Sbjct: 181 GKIVAMKKVRFVNMDPESVRFMAREILILRKLDHPNVMKLEGLVTSRLSGSLYLVFEYME 240

Query: 241 HDLAGLAAAPGHKFTEAQIKCFVQQLLHGLEHCHSRGVLHRDIKGSNLLVDSNGVLKIGD 300
           HDLAGLAA PG KF+E QIKC++QQL  GLEHCH RG+LHRDIKGSNLL+++ GVLKIGD
Sbjct: 241 HDLAGLAATPGIKFSEPQIKCYMQQLFRGLEHCHRRGILHRDIKGSNLLINNEGVLKIGD 300

Query: 301 FGLATFFKPEQKQPLTSRVVTLWYRAPELLLGATDYGPAIDLWSCGCIVAELFAGKPIMP 360
           FGLA F++ +    LTSRVVTLWYRAPELLLGAT+YGPAIDLWS GCI+ ELFAGKPIMP
Sbjct: 301 FGLANFYRGDGDLQLTSRVVTLWYRAPELLLGATEYGPAIDLWSAGCILTELFAGKPIMP 360

Query: 361 GRTEVEQMHKIFKLCGSPSEEFWRRTKLPHATSFKPHHPYKSCLSETFKNFPSSALALVN 420
           GRTEVEQMHKIFKLCGSPSE++WRR  LP ATSFKP HPYK  L+ETF +FPSSAL L+N
Sbjct: 361 GRTEVEQMHKIFKLCGSPSEDYWRRATLPLATSFKPSHPYKPVLAETFNHFPSSALMLIN 420

Query: 421 KLLAIEPEHRGSATSALSSEFFITEPLPCDPSSLPKYPPSKEYDAKLRNEEERK 462
           KLLAIEPE RGSA S L SEFF TEPLP +PS+LP+YPPSKE DAKLRNEE RK
Sbjct: 421 KLLAIEPEKRGSAASTLRSEFFTTEPLPANPSNLPRYPPSKELDAKLRNEEARK 463

BLAST of MS006336 vs. TAIR 10
Match: AT1G71530.2 (Protein kinase superfamily protein )

HSP 1 Score: 582.0 bits (1499), Expect = 4.2e-166
Identity = 317/474 (66.88%), Postives = 351/474 (74.05%), Query Frame = 0

Query: 1   MGCICSRSSSEPEDSNQKKSEKDLNKSSVQLVAPSPSKGEDLLLEIQLSRQASKVKGGSV 60
           MGCIC+ + S P  +   K   D +KS +QL+   P            S  +SK +G   
Sbjct: 1   MGCICATARS-PSAAVTDKDLLDSSKSILQLLPHHPPS----------SSSSSKKEGSFT 60

Query: 61  HKTK-------ANGGSHHHR--AKDDEAENKSVKAGKANNDNASTGGKQPEVFRLASLSK 120
             T        ANG     R     D    K V    A   N +       V + A    
Sbjct: 61  RTTSSASITIVANGYPVARRPSTSSDRNSTKPVVVVGAPTRNPTRRVTAIPVAQPAQQQP 120

Query: 121 AA----ETELVMAGWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLET 180
           A     +TEL  A WPSWLASVAGEAIKGWVPR A+SFEKLDKIGQGTYSSVYKARDLET
Sbjct: 121 ARVISNKTELPAAEWPSWLASVAGEAIKGWVPRCAESFEKLDKIGQGTYSSVYKARDLET 180

Query: 181 GKTVALKKVRFVNMDPESVRFMAREIYILRKLDHPNVMKLECLVTSRVSGSLYLVFEYME 240
           GK VA+KKVRFVNMDPESVRFMAREI ILRKLDHPNVMKLE LVTSR+SGSLYLVFEYME
Sbjct: 181 GKIVAMKKVRFVNMDPESVRFMAREILILRKLDHPNVMKLEGLVTSRLSGSLYLVFEYME 240

Query: 241 HDLAGLAAAPGHKFTEAQIKCFVQQLLHGLEHCHSRGVLHRDIKGSNLLVDSNGVLKIGD 300
           HDLAGLAA PG KF+E QIKC++QQL  GLEHCH RG+LHRDIKGSNLL+++ GVLKIGD
Sbjct: 241 HDLAGLAATPGIKFSEPQIKCYMQQLFRGLEHCHRRGILHRDIKGSNLLINNEGVLKIGD 300

Query: 301 FGLATFFKPEQKQPLTSRVVTLWYRAPELLLGATDYGPAIDLWSCGCIVAELFAGKPIMP 360
           FGLA F++ +    LTSRVVTLWYRAPELLLGAT+YGPAIDLWS GCI+ ELFAGKPIMP
Sbjct: 301 FGLANFYRGDGDLQLTSRVVTLWYRAPELLLGATEYGPAIDLWSAGCILTELFAGKPIMP 360

Query: 361 GRTEVEQMHKIFKLCGSPSEEFWRRTKLPHATSFKPHHPYKSCLSETFKNFPSSALALVN 420
           GRTEVEQMHKIFKLCGSPSE++WRR  LP ATSFKP HPYK  L+ETF +FPSSAL L+N
Sbjct: 361 GRTEVEQMHKIFKLCGSPSEDYWRRATLPLATSFKPSHPYKPVLAETFNHFPSSALMLIN 420

Query: 421 KLLAIEPEHRGSATSALSSEFFITEPLPCDPSSLPKYPPSKEYDAKLRNEEERK 462
           KLLAIEPE RGSA S L SEFF TEPLP +PS+LP+YPPSKE DAKLRNEE RK
Sbjct: 421 KLLAIEPEKRGSAASTLRSEFFTTEPLPANPSNLPRYPPSKELDAKLRNEEARK 463

BLAST of MS006336 vs. TAIR 10
Match: AT1G53050.1 (Protein kinase superfamily protein )

HSP 1 Score: 569.7 bits (1467), Expect = 2.1e-162
Identity = 289/461 (62.69%), Postives = 352/461 (76.36%), Query Frame = 0

Query: 1   MGCICSRSSSEPEDSNQKKSEKDLNKSSVQLVAPSPSKGEDLLLEIQLSRQASKVKGGSV 60
           MGC+C + S+  EDS     E+  +KSS +     P         +++  ++  V    V
Sbjct: 1   MGCVCGKPSA-IEDSKDSPRERFSSKSSSEFRVSRPVASSRREEPLRIKERSDVVSVRPV 60

Query: 61  HKTKANGGSHHHRAKDDEAENKSVKAGKANNDNASTGGKQPEVFRLASLSKAAETELVMA 120
              K +  S H R ++     K ++   A +  A +           +++KA E E V A
Sbjct: 61  LSNKQSNVSLHLRGENLSRREKRIENVAATSPLAMS----------ITIAKATEGEYVAA 120

Query: 121 GWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSSVYKARDLETGKTVALKKVRFVN 180
           GWP WLASVAGEAI+GWVPRRADSFEKLDKIGQGTYS+VY+ARDL+  K VALKKVRF N
Sbjct: 121 GWPPWLASVAGEAIRGWVPRRADSFEKLDKIGQGTYSNVYRARDLDQKKIVALKKVRFDN 180

Query: 181 MDPESVRFMAREIYILRKLDHPNVMKLECLVTSRVSGSLYLVFEYMEHDLAGLAAAPGHK 240
           ++PESVRFMAREI ILR+LDHPN++KLE LVTSR+S SLYLVFEYMEHDLAGLA+ P  K
Sbjct: 181 LEPESVRFMAREIQILRRLDHPNIIKLEGLVTSRMSCSLYLVFEYMEHDLAGLASHPAIK 240

Query: 241 FTEAQIKCFVQQLLHGLEHCHSRGVLHRDIKGSNLLVDSNGVLKIGDFGLATFFKPEQKQ 300
           F+E+Q+KC++QQLLHGL+HCHSRGVLHRDIKGSNLL+D++GVLKI DFGLA+FF P Q Q
Sbjct: 241 FSESQVKCYLQQLLHGLDHCHSRGVLHRDIKGSNLLIDNSGVLKIADFGLASFFDPRQTQ 300

Query: 301 PLTSRVVTLWYRAPELLLGATDYGPAIDLWSCGCIVAELFAGKPIMPGRTEVEQMHKIFK 360
           PLTSRVVTLWYR PELLLGAT YG A+DLWS GCI+AEL+AGKPIMPGRTEVEQ+HKIFK
Sbjct: 301 PLTSRVVTLWYRPPELLLGATRYGAAVDLWSAGCILAELYAGKPIMPGRTEVEQLHKIFK 360

Query: 361 LCGSPSEEFWRRTKLPHATSFKPHHPYKSCLSETFKNFPSSALALVNKLLAIEPEHRGSA 420
           LCGSP+E++W +++LPHAT FKP  PYK  + ETFK FP  ALAL+  LL++ P+ RG+A
Sbjct: 361 LCGSPTEDYWVKSRLPHATIFKPTQPYKRLVGETFKEFPQPALALLETLLSVNPDDRGTA 420

Query: 421 TSALSSEFFITEPLPCDPSSLPKYPPSKEYDAKLRNEEERK 462
           T+AL SEFF T PLPCDPSSLPKYPPSKE DA++R+EE R+
Sbjct: 421 TAALKSEFFSTRPLPCDPSSLPKYPPSKELDARMRDEESRR 450

BLAST of MS006336 vs. TAIR 10
Match: AT1G09600.1 (Protein kinase superfamily protein )

HSP 1 Score: 566.2 bits (1458), Expect = 2.4e-161
Identity = 298/483 (61.70%), Postives = 355/483 (73.50%), Query Frame = 0

Query: 1   MGCICSRSSSEPEDSNQKKSEKDLNKSSVQLVAPSPSKGEDLLLEIQLSRQASKVKGGSV 60
           MGC C++  + P++ N   S   ++  +V+     P K      + + S+ AS  K  +V
Sbjct: 1   MGCNCTK-GTRPDNDNVDNSNSIVSNVNVKERRSKPKKTPK---KKKKSKSASSSKDNNV 60

Query: 61  -HKTKANGGSHH--------HRAKDDEAENK---SVKAGKANNDNASTG----------G 120
             + ++N                KDDE+E K     K+ +       TG           
Sbjct: 61  GFEERSNDNKEASLTLLIPIDAKKDDESEKKVNLERKSSRLVFQRRPTGIEVGANNIGTL 120

Query: 121 KQPEVFRLASLSKAAETELVMAGWPSWLASVAGEAIKGWVPRRADSFEKLDKIGQGTYSS 180
           +QP++ R+ S+S       VMAGWPSWLASVAGEAI GW+PR+ADSFEKL+KIGQGTYSS
Sbjct: 121 QQPKMTRICSVSNGERGAQVMAGWPSWLASVAGEAINGWIPRKADSFEKLEKIGQGTYSS 180

Query: 181 VYKARDLETGKTVALKKVRFVNMDPESVRFMAREIYILRKLDHPNVMKLECLVTSRVSGS 240
           VYKARDLET + VALKKVRF NMDP+SVRFMAREI ILR+LDHPNVMKLE L+TSRVSGS
Sbjct: 181 VYKARDLETNQLVALKKVRFANMDPDSVRFMAREIIILRRLDHPNVMKLEGLITSRVSGS 240

Query: 241 LYLVFEYMEHDLAGLAAAPGHKFTEAQIKCFVQQLLHGLEHCHSRGVLHRDIKGSNLLVD 300
           +YL+FEYMEHDLAGLA+ PG  F+EAQIKC+++QLLHGLEHCHSRGVLHRDIKGSNLL+D
Sbjct: 241 MYLIFEYMEHDLAGLASTPGINFSEAQIKCYMKQLLHGLEHCHSRGVLHRDIKGSNLLLD 300

Query: 301 SNGVLKIGDFGLATFFKPEQKQPLTSRVVTLWYRAPELLLGATDYGPAIDLWSCGCIVAE 360
            N  LKIGDFGLA F++  QKQPLTSRVVTLWYR PELLLG+TDYG  +DLWS GCI+AE
Sbjct: 301 HNNNLKIGDFGLANFYQGHQKQPLTSRVVTLWYRPPELLLGSTDYGVTVDLWSTGCILAE 360

Query: 361 LFAGKPIMPGRTEVEQMHKIFKLCGSPSEEFWRRTKLPHATSFKPHHPYKSCLSETFKNF 420
           LF GKPIMPGRTEVEQ+HKIFKLCGSPSEE+W+ +KLPHAT FKP  PYK C++ETFK+ 
Sbjct: 361 LFTGKPIMPGRTEVEQLHKIFKLCGSPSEEYWKISKLPHATIFKPQQPYKRCVAETFKSL 420

Query: 421 PSSALALVNKLLAIEPEHRGSATSALSSEFFITEPLPCDPSSLPKYPPSKEYDAKLRNEE 462
           PSSALALV  LLA+EP+ RG+  SAL SEFF T PL  DPSSLPKY P KE D K + EE
Sbjct: 421 PSSALALVEVLLAVEPDARGTTASALESEFFTTSPLASDPSSLPKYQPRKEIDVKAQEEE 479

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141899.11.1e-264100.00probable serine/threonine-protein kinase At1g09600 [Momordica charantia][more]
XP_038889815.17.4e-24291.11probable serine/threonine-protein kinase At1g09600 isoform X1 [Benincasa hispida... [more]
XP_011656099.12.4e-24090.67probable serine/threonine-protein kinase At1g09600 isoform X1 [Cucumis sativus][more]
KAA0039258.11.5e-23990.46putative serine/threonine-protein kinase [Cucumis melo var. makuwa] >TYK00444.1 ... [more]
XP_008459627.16.5e-23890.02PREDICTED: probable serine/threonine-protein kinase At1g09600 [Cucumis melo] >XP... [more]
Match NameE-valueIdentityDescription
F4I1143.3e-16061.70Probable serine/threonine-protein kinase At1g09600 OS=Arabidopsis thaliana OX=37... [more]
Q9ZVM95.0e-14863.55Probable serine/threonine-protein kinase At1g54610 OS=Arabidopsis thaliana OX=37... [more]
F4ICB61.5e-13664.27Protein IMPAIRED IN BABA-INDUCED STERILITY 1 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q5JK682.6e-9651.61Cyclin-dependent kinase C-2 OS=Oryza sativa subsp. japonica OX=39947 GN=CDKC-2 P... [more]
Q9LFT82.2e-9551.18Cyclin-dependent kinase C-1 OS=Arabidopsis thaliana OX=3702 GN=CDKC-1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CK275.2e-265100.00probable serine/threonine-protein kinase At1g09600 OS=Momordica charantia OX=367... [more]
A0A0A0KSU91.2e-24090.67Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G650... [more]
A0A5D3BMF17.5e-24090.46Putative serine/threonine-protein kinase OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A1S3CAR03.2e-23890.02probable serine/threonine-protein kinase At1g09600 OS=Cucumis melo OX=3656 GN=LO... [more]
A0A6J1GBD68.3e-22383.47probable serine/threonine-protein kinase At1g54610 OS=Cucurbita moschata OX=3662... [more]
Match NameE-valueIdentityDescription
AT1G33770.13.5e-17367.73Protein kinase superfamily protein [more]
AT1G71530.14.2e-16666.88Protein kinase superfamily protein [more]
AT1G71530.24.2e-16666.88Protein kinase superfamily protein [more]
AT1G53050.12.1e-16262.69Protein kinase superfamily protein [more]
AT1G09600.12.4e-16161.70Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 145..429
e-value: 9.4E-95
score: 330.8
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 145..429
e-value: 5.1E-70
score: 235.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 145..429
score: 45.219627
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 228..461
e-value: 3.1E-73
score: 248.2
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 132..227
e-value: 4.0E-27
score: 96.1
NoneNo IPR availablePIRSRPIRSR630220-2PIRSR630220-2coord: 146..358
e-value: 1.5E-12
score: 44.3
NoneNo IPR availablePIRSRPIRSR037014-2PIRSR037014-2coord: 150..342
e-value: 1.4E-28
score: 97.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 52..99
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..36
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 434..461
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 70..85
NoneNo IPR availablePANTHERPTHR24056CELL DIVISION PROTEIN KINASEcoord: 72..461
NoneNo IPR availablePANTHERPTHR24056:SF384PROTEIN KINASE SUPERFAMILY PROTEINcoord: 72..461
NoneNo IPR availableCDDcd07840STKc_CDK9_likecoord: 145..429
e-value: 6.15563E-164
score: 462.034
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 265..277
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 151..174
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 138..437

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS006336.1MS006336.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity