Homology
BLAST of MS006200 vs. NCBI nr
Match:
XP_022158049.1 (probable pectinesterase/pectinesterase inhibitor 61 [Momordica charantia])
HSP 1 Score: 1163.3 bits (3008), Expect = 0.0e+00
Identity = 592/597 (99.16%), Postives = 594/597 (99.50%), Query Frame = 0
Query: 5 MNYGKLHSTSSGETPANFELSNSNSSISSPNRRTRTRVVLLSLLSILLITASAISAVVFV 64
MNYGKLHSTSSG+TPANFELSNSNSSISSPNRRTRTRVVLLSLLSILLI ASAISAVVFV
Sbjct: 1 MNYGKLHSTSSGDTPANFELSNSNSSISSPNRRTRTRVVLLSLLSILLIMASAISAVVFV 60
Query: 65 RSHIGRSGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALTADVHDLVHISLNV 124
RSHIGR GESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGAL ADVHDLVHISLNV
Sbjct: 61 RSHIGRGGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALAADVHDLVHISLNV 120
Query: 125 TLQRLTKALYSSSQIPVSQISDPLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDVLTW 184
TLQRLTKALYSSSQIPVSQISDPLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDVLTW
Sbjct: 121 TLQRLTKALYSSSQIPVSQISDPLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDVLTW 180
Query: 185 LSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPV 244
LSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPV
Sbjct: 181 LSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPV 240
Query: 245 QNRRRLMEAEWPQENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAI 304
QNRRRLMEAEWPQENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAI
Sbjct: 241 QNRRRLMEAEWPQENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAI 300
Query: 305 AAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFR 364
AAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFR
Sbjct: 301 AAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFR 360
Query: 365 TATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQ 424
TATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQ
Sbjct: 361 TATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQ 420
Query: 425 FYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACK 484
FYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACK
Sbjct: 421 FYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACK 480
Query: 485 ILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGE 544
ILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGE
Sbjct: 481 ILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGE 540
Query: 545 YMNSGPGAAVGQRVKWPGYRVITSTIEAGKFTVGQFIYGSAWLPSTGVSFSAGLSAS 602
YMNSGPGAAVGQRVKWPGYRVITST+EAGKFTVGQFIYGSAWLPSTGVSFSAGLSAS
Sbjct: 541 YMNSGPGAAVGQRVKWPGYRVITSTVEAGKFTVGQFIYGSAWLPSTGVSFSAGLSAS 597
BLAST of MS006200 vs. NCBI nr
Match:
XP_022957644.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita moschata])
HSP 1 Score: 921.8 bits (2381), Expect = 3.1e-264
Identity = 478/603 (79.27%), Postives = 530/603 (87.89%), Query Frame = 0
Query: 6 NYGKLHSTSSGETPANFELSNSNSSISSPNRRTRTRVVLLSLLSILLITASAISAVVFVR 65
NY KLHS++SG+TPA F++S++ + SS R RTR++ +SL+SILLITASA+SA V +
Sbjct: 3 NYNKLHSSASGDTPATFQVSHTPNPPSSSASRRRTRILFVSLISILLITASAVSAAVLLH 62
Query: 66 SHIGRSGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALTADVHDLVHISLNVT 125
S +S +KP+QAIS ACS TLYP++C SSL+ FPG+L+ADVH LVHISLN+T
Sbjct: 63 S------QSSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISLNLT 122
Query: 126 LQRLTKALYSSSQIPVSQIS-DPLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDVLTW 185
LQ LTKALY+SSQIPV QIS +PL SA+ DCIELL+DAVDAFS SLFST+ASNHD++TW
Sbjct: 123 LQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTW 182
Query: 186 LSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPV 245
LSAALTYHDTC++G ADV VKDQM A+L+DLS++ISNSLAIFSG+G G+PV
Sbjct: 183 LSAALTYHDTCSDGFADVD-DPVKDQMAARLSDLSEMISNSLAIFSGYG------EGLPV 242
Query: 246 QNRRRLMEA--EWPQENG----GEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYK 305
QNRRRLME+ EW ENG EFP WVS KDR+LL +PISAIQADIVVAKDG+GK+K
Sbjct: 243 QNRRRLMESDREWAAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFK 302
Query: 306 TVAEAIAAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFD 365
TVAEAI AAPSSSGRR VIY+KAGKY EENLKVGRKKTNLMFVGDGKGITVISGGKSVFD
Sbjct: 303 TVAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFD 362
Query: 366 KVTTFRTATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLY 425
KVTTFRTATFA SGTGIILRDMTFENTAGP +HQAVALRVSADHAVV+RCNIIGYQDTLY
Sbjct: 363 KVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRVSADHAVVYRCNIIGYQDTLY 422
Query: 426 VHSNRQFYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGI 485
VHSNRQF+RECDIYGTVDFIFGNAVVVFQSCNIYARKPMP QKNTITAQ+RKDPNQNTGI
Sbjct: 423 VHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQNTGI 482
Query: 486 SIHACKILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD 545
SIHACKILA +DLE SKGS+PTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD
Sbjct: 483 SIHACKILATTDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD 542
Query: 546 TLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEAGKFTVGQFIYGSAWLPSTGVSFSAGL 602
TLYYGEYMNSGPGAA GQRV WPGYRVITSTIEA KFTVGQFIYGS+WLPSTGVSFSAGL
Sbjct: 543 TLYYGEYMNSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFSAGL 592
BLAST of MS006200 vs. NCBI nr
Match:
XP_023533011.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 918.3 bits (2372), Expect = 3.4e-263
Identity = 474/603 (78.61%), Postives = 526/603 (87.23%), Query Frame = 0
Query: 6 NYGKLHSTSSGETPANFELSNSNSSISSPNRRTRTRVVLLSLLSILLITASAISAVVFVR 65
NY KLHS+ SG+TPA F++S++ + SS R RTR++ +SL+SILLITASA+SA V +
Sbjct: 3 NYNKLHSSGSGDTPATFQVSHTPNPPSSSASRRRTRILFVSLISILLITASAVSAAVLIH 62
Query: 66 SHIGRSGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALTADVHDLVHISLNVT 125
++ +KP+QAIS ACS TLYP++C SSL+ FPG+L+ADVH LVHISLN+T
Sbjct: 63 TNTSHFPH------NKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHHLVHISLNLT 122
Query: 126 LQRLTKALYSSSQIPVSQIS-DPLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDVLTW 185
LQ LTKALY+SSQIPV QIS +PL SA+ DCIELL+DAVDAFS SLFST+ASNHD++TW
Sbjct: 123 LQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTW 182
Query: 186 LSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPV 245
LSAALTYHDTC++G ADV VKDQM A+L+DLS++ISNSLAIFSG+G G+PV
Sbjct: 183 LSAALTYHDTCSDGFADVD-DPVKDQMAARLSDLSEMISNSLAIFSGYG------QGLPV 242
Query: 246 QNRRRLME--AEWPQENG----GEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYK 305
QNRRRLME +EW ENG EFP WVS KDR+LL +PISAIQADIVVAKDG+GK+
Sbjct: 243 QNRRRLMESDSEWAAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFT 302
Query: 306 TVAEAIAAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFD 365
TVAEAI AAPSSSGRR VIY+KAGKY EENLKVGRKKTNLMFVGDGKGITVISGGKSVFD
Sbjct: 303 TVAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFD 362
Query: 366 KVTTFRTATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLY 425
KVTTFRTATFA SGTGIILRDMTFENTAGP +HQAVALRVSADHAVV+RCNIIGYQDTLY
Sbjct: 363 KVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRVSADHAVVYRCNIIGYQDTLY 422
Query: 426 VHSNRQFYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGI 485
VHSNRQF+RECDIYGTVDFIFGNAVVVFQSCNIYARKPMP QKNTITAQ+RKDPNQNTGI
Sbjct: 423 VHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQNTGI 482
Query: 486 SIHACKILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD 545
SIHACKILA +DLE SKGS+PTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD
Sbjct: 483 SIHACKILATTDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD 542
Query: 546 TLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEAGKFTVGQFIYGSAWLPSTGVSFSAGL 602
TLYYGEYMNSGPGAA GQRV WPGYRVITSTIEA KFTVGQFIYGS+WLPSTGVSFSAGL
Sbjct: 543 TLYYGEYMNSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFSAGL 592
BLAST of MS006200 vs. NCBI nr
Match:
KAG6605784.1 (putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 915.2 bits (2364), Expect = 2.9e-262
Identity = 473/603 (78.44%), Postives = 524/603 (86.90%), Query Frame = 0
Query: 6 NYGKLHSTSSGETPANFELSNSNSSISSPNRRTRTRVVLLSLLSILLITASAISAVVFVR 65
NY KLHS++SG+TPA F++S++ + SS R RTR++ +SL+SILLI SA+SA V +
Sbjct: 3 NYNKLHSSASGDTPATFQVSHTPNPPSSSASRRRTRILFVSLISILLIAVSAVSAAVLIH 62
Query: 66 SHIGRSGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALTADVHDLVHISLNVT 125
SH +KP+QAIS ACS TLYP++C SSL+ FPG+L+ADVH LVHISLN+T
Sbjct: 63 SHTSHFPH------NKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISLNLT 122
Query: 126 LQRLTKALYSSSQIPVSQIS-DPLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDVLTW 185
LQ LTKALY+SSQIPV QIS +PL SA+ DCIELL+DAVDAFS SLFST+ASNHD++TW
Sbjct: 123 LQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTW 182
Query: 186 LSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPV 245
LSAALTYHDTC++G ADV VKDQM A+L+DLS++ISNSLAIFSG+G +PV
Sbjct: 183 LSAALTYHDTCSDGFADVD-DPVKDQMAARLSDLSEMISNSLAIFSGYG------EELPV 242
Query: 246 QNRRRLMEA--EWPQENG----GEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYK 305
QNRRRLME+ EW ENG EFP WVS KDR+LL +PISAIQADIVVAKDG+GK+
Sbjct: 243 QNRRRLMESDREWVAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFT 302
Query: 306 TVAEAIAAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFD 365
TVAEAI AAPSSSGRR VIY+KAGKY EENLKVGRKKTNLMFVGDGKGITVISGGKSVFD
Sbjct: 303 TVAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFD 362
Query: 366 KVTTFRTATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLY 425
KVTTFRTATFA SGTGIILRDMTFENTAGP +HQAVALRVSADHAVV+RCNIIGYQDTLY
Sbjct: 363 KVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRVSADHAVVYRCNIIGYQDTLY 422
Query: 426 VHSNRQFYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGI 485
VHSNRQF+RECDIYGTVDFIFGNAVVVFQSCNIYARKPMP QKNTITAQ+RKDPNQNTGI
Sbjct: 423 VHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQNTGI 482
Query: 486 SIHACKILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD 545
SIHACKILA +DLE SKGS+PTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD
Sbjct: 483 SIHACKILATTDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD 542
Query: 546 TLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEAGKFTVGQFIYGSAWLPSTGVSFSAGL 602
TLYYGEYMNSGPGAA GQRV WPGYRVITSTIEA KFTVGQFIYGS+WLPSTGVSFSAGL
Sbjct: 543 TLYYGEYMNSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFSAGL 592
BLAST of MS006200 vs. NCBI nr
Match:
KAG6579321.1 (putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 914.1 bits (2361), Expect = 6.5e-262
Identity = 474/606 (78.22%), Postives = 533/606 (87.95%), Query Frame = 0
Query: 6 NYGKLHSTSSGETPA-NFELSNSNSSISSP--NRRTRTRVVLLSLLSILLITASAISAVV 65
NY KLHS+ SG+TPA +F +SN +S P +R R++L+S LSI LITASA+SAVV
Sbjct: 4 NYNKLHSSRSGDTPASSFRVSNDHSDPLPPPSPSSSRRRILLISFLSIFLITASAVSAVV 63
Query: 66 FVRSHIGRSGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALTADVHDLVHISL 125
V S I S ES +KP+QAISRACS TLYP++C+SSL+ PG+L+ADVHDLVHISL
Sbjct: 64 LVHSRIAASTESHFP-HNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDLVHISL 123
Query: 126 NVTLQRLTKALYSSSQIPVSQIS-DPLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDV 185
N+TLQ LTKALYS+SQIP+SQIS DPL SA+ DC+ELL+DAV+AFS SLFST A+NHDV
Sbjct: 124 NLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTATNHDV 183
Query: 186 LTWLSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSG 245
+TWLSAALTYHDTCT+G +DV A +KDQ+TAKL+DLS++ISNSLAIFSGFGG
Sbjct: 184 MTWLSAALTYHDTCTDGFSDV-ADPIKDQITAKLSDLSEMISNSLAIFSGFGG-----DD 243
Query: 246 VPVQN--RRRLMEAE--W--PQENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTG 305
+PV+N RRRLME++ W + NG +FP+WVS KDR+LL +PIS+IQADIVVAKDG+G
Sbjct: 244 LPVENRRRRRLMESDEHWLAEENNGDGDFPQWVSGKDRRLLAAPISSIQADIVVAKDGSG 303
Query: 306 KYKTVAEAIAAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKS 365
K+KTVAEAIAAAPSSSGRR VIY+KAGKY EENLKVGRKKTNLMFVGDGKGITVISGGKS
Sbjct: 304 KFKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKS 363
Query: 366 VFDKVTTFRTATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQD 425
V+DKVTTFRTATFA SGTG+ILRDMTFENTAGP +HQAVALR+SADHAVVFR N+IGYQD
Sbjct: 364 VYDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVIGYQD 423
Query: 426 TLYVHSNRQFYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQN 485
TLYVHSNRQF+RECDIYGT+DFIFGNA VVFQSCNIYARKPM GQKNTITAQDRKDPNQN
Sbjct: 424 TLYVHSNRQFFRECDIYGTIDFIFGNAAVVFQSCNIYARKPMSGQKNTITAQDRKDPNQN 483
Query: 486 TGISIHACKILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF 545
TGISIHACKI+A DLE SKGS+PT+LGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF
Sbjct: 484 TGISIHACKIIATGDLEASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF 543
Query: 546 ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEAGKFTVGQFIYGSAWLPSTGVSFS 602
ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEA KFTVGQFIYGS+WLPSTGVSFS
Sbjct: 544 ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFS 602
BLAST of MS006200 vs. ExPASy Swiss-Prot
Match:
Q9FK05 (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3702 GN=PME61 PE=2 SV=1)
HSP 1 Score: 726.1 bits (1873), Expect = 3.3e-208
Identity = 366/588 (62.24%), Postives = 453/588 (77.04%), Query Frame = 0
Query: 19 PANFELSNSNSSISSPNRRTRTRVVLLSLLSILLITASAISAVVFVRSHIGRSGESGLGL 78
P+N + +S+ ++T+T+++L +L ++ + +F SG++ L
Sbjct: 11 PSNPNQKDPATSLPELQKKTKTKLILFTL---AVLVVGVVCFGIFAGIRAVDSGKTEPKL 70
Query: 79 AHKPSQAISRACSRTLYPAVCVSSLVEFPGALTADVHDLVHISLNVTLQRLTKALYSSSQ 138
KP+QAISR CS++LYP +C+ +L++FPG+LTAD ++L+HIS N TLQ+ +KALY+SS
Sbjct: 71 TRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSST 130
Query: 139 IPVSQISDPLLLSAFADCIELLDDAVDAFSRSLFSTAA-----SNHDVLTWLSAALTYHD 198
I +Q+ P + SA+ C+ELLDD+VDA +R+L S S+ DV+TWLS+A+T HD
Sbjct: 131 ITYTQM-PPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTNHD 190
Query: 199 TCTEGLADV--SAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPVQNRRRLM 258
TCT+G ++ G+VKDQ+ + DLS+++SN LAIF+ G + SGVPV N R+L+
Sbjct: 191 TCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFA---GKVKDLSGVPVVNNRKLL 250
Query: 259 EAEWPQENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAIAAAPSSS 318
E EE P W+ R+DR+LLG+P SAIQADI V+KDG+G +KT+AEAI AP S
Sbjct: 251 GTE-----ETEELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHS 310
Query: 319 GRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAAS 378
RR VIYVKAG+Y EENLKVGRKKTNLMF+GDGKG TVI+GGKS+ D +TTF TATFAA+
Sbjct: 311 SRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAAT 370
Query: 379 GTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQFYRECDI 438
G G I+RDMTFEN AGP +HQAVALRV DHAVV+RCNIIGYQD LYVHSNRQF+REC+I
Sbjct: 371 GAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEI 430
Query: 439 YGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKILAASDL 498
YGTVDFIFGNA V+ QSCNIYARKPM QK TITAQ+RKDPNQNTGISIHACK+LA DL
Sbjct: 431 YGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDL 490
Query: 499 EGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPG 558
E SKGSYPT+LGRPWKLYSRVVYM+S MGDHI PRGWLEW G FALD+LYYGEYMN G G
Sbjct: 491 EASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLG 550
Query: 559 AAVGQRVKWPGYRVITSTIEAGKFTVGQFIYGSAWLPSTGVSFSAGLS 600
+ +GQRVKWPGY VITST+EA KFTV QFI GS+WLPSTGVSF +GLS
Sbjct: 551 SGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586
BLAST of MS006200 vs. ExPASy Swiss-Prot
Match:
Q9M3B0 (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3702 GN=PME34 PE=2 SV=1)
HSP 1 Score: 699.5 bits (1804), Expect = 3.3e-200
Identity = 368/593 (62.06%), Postives = 453/593 (76.39%), Query Frame = 0
Query: 12 STSSGETPANFELSNSNSSISSPNRRTRTRVVLLSL-LSILLITASAISAVVFVRSHIGR 71
+TS+ P ++S S + NRR++ ++V+ S+ L+I LI A+AI A VRS + +
Sbjct: 16 TTSTTTAPILNQVSTSEQP-ENNNRRSKKKLVVSSIVLAISLILAAAIFA--GVRSRL-K 75
Query: 72 SGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALTA-DVHDLVHISLNVTLQRL 131
+S GLA KPSQAIS+AC T +P +CV SL++FPG+L A DL+H+++N+TL
Sbjct: 76 LNQSVPGLARKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHF 135
Query: 132 TKALYSSSQIPVSQISDPLLLSAFADCIELLDDAVDAFSRSLFSTAASN---HDVLTWLS 191
+ ALYSS+ + + P SA+ C+ELLDD+VDA SR+L S +S+ DV TWLS
Sbjct: 136 SHALYSSASLSFVDM-PPRARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLS 195
Query: 192 AALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPVQN 251
AALT HDTCTEG V G VKD MTA L +LS+L+SN LAIFS G +F+GVP+QN
Sbjct: 196 AALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDG-DDFAGVPIQN 255
Query: 252 RRRLMEAEWPQENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAIAA 311
RR L E E+FP W+ K+R++L P+S IQADI+V+KDG G KT++EAI
Sbjct: 256 RRLL-----GVEEREEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKK 315
Query: 312 APSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTA 371
AP +S RR +IYVKAG+Y E NLKVGRKK NLMFVGDGKG TVISGGKS+FD +TTF TA
Sbjct: 316 APQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTA 375
Query: 372 TFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQFY 431
+FAA+G G I RD+TFEN AGP +HQAVALR+ ADHAV++RCNIIGYQDTLYVHSNRQF+
Sbjct: 376 SFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFF 435
Query: 432 RECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKIL 491
RECDIYGTVDFIFGNA VV Q+C+IYARKPM QKNTITAQ+RKDPNQNTGISIHA ++L
Sbjct: 436 RECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVL 495
Query: 492 AASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYM 551
AASDL+ + GS T+LGRPWKL+SR VYM+S +G H+H RGWLEW +FALDTLYYGEY+
Sbjct: 496 AASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYL 555
Query: 552 NSGPGAAVGQRVKWPGYRVITSTIEAGKFTVGQFIYGSAWLPSTGVSFSAGLS 600
NSGPG+ +GQRV WPGYRVI ST EA +FTV +FIYGS+WLPSTGVSF AGLS
Sbjct: 556 NSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 597
BLAST of MS006200 vs. ExPASy Swiss-Prot
Match:
O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)
HSP 1 Score: 441.4 bits (1134), Expect = 1.6e-122
Identity = 257/589 (43.63%), Postives = 363/589 (61.63%), Query Frame = 0
Query: 23 ELSNSNSSISSPNRRTRTRVVLLSLLSILLITASAISAVVFVRSHIGRSGESGLGLAHKP 82
+LS S+S+ + N + + + L+L + LL+ A+ I V V S SG++G ++P
Sbjct: 10 KLSESSSNQNISNIPKKKKKLFLALFATLLVVAAVIGIVAGVNSR-KNSGDNG----NEP 69
Query: 83 SQAISR-ACSRTLYPAVCVSSLVEFPGA--LTADVHDLVHISLNVTLQRLTKALYSSSQI 142
AI + +CS T YP +C S++ P A D++ +SLN+T + + ++
Sbjct: 70 HHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKL 129
Query: 143 PVSQISDPLLLSAFADCIELLDDAVDAFSRSL-------FSTAASNH--DVLTWLSAALT 202
A DC+E +D+ +D +++ + S H D+ T +SAA+T
Sbjct: 130 LKRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMT 189
Query: 203 YHDTCTEGLADVSAGQ-VKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPVQNRRR 262
TC +G + A + V+D ++ + ++ SN+LA+ + + N R+
Sbjct: 190 NQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNM--TDTDMMIMRTSNNRK 249
Query: 263 LMEAEWPQENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAIAAAPS 322
L E + + + +P W+S DR+LL S S++ + VVA DG+G +KTVA A+AAAP
Sbjct: 250 LTE----ETSTVDGWPAWLSPGDRRLLQS--SSVTPNAVVAADGSGNFKTVAAAVAAAPQ 309
Query: 323 SSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFA 382
+R +I +KAG Y EN++V +K N+MF+GDG+ T+I+G ++V D TTF++AT A
Sbjct: 310 GGTKRYIIRIKAGVY-RENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAA 369
Query: 383 ASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQFYREC 442
G G + RD+TF+NTAGP +HQAVALRV AD + + C+++ YQDTLYVHSNRQF+ C
Sbjct: 370 VVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNC 429
Query: 443 DIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKILAAS 502
I GTVDFIFGNA V Q+C+I+ARKP GQKN +TAQ R DPNQNTGI I +I A S
Sbjct: 430 LIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATS 489
Query: 503 DLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSG 562
DL+ +GS+PT+LGRPWK YSR V M SS+ D IHP GW EW G+FAL+TL+YGE+ NSG
Sbjct: 490 DLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSG 549
Query: 563 PGAAVGQRVKWPGYRVITSTIEAGKFTVGQFIYGSAWLPSTGVSFSAGL 599
GA RVKW G+RVITS EA FT G FI GS+WL STG FS GL
Sbjct: 550 AGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584
BLAST of MS006200 vs. ExPASy Swiss-Prot
Match:
P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)
HSP 1 Score: 441.0 bits (1133), Expect = 2.1e-122
Identity = 254/589 (43.12%), Postives = 365/589 (61.97%), Query Frame = 0
Query: 23 ELSNSNSSISSPNRRTRTRVVLLSLLSILLITASAISAVVFVRSHIGRSGESGLGLAHKP 82
+LS S+++ + N + + + L+L + LL+ A+ I V V S SG++G ++P
Sbjct: 10 KLSESSTNQNISNIPKKKKKLFLALFATLLVVAAVIGIVAGVNSR-KNSGDNG----NEP 69
Query: 83 SQAISR-ACSRTLYPAVCVSSLVEFPGA--LTADVHDLVHISLNVTLQRLTKALYSSSQI 142
AI + +CS T YP +C S++ P A D++ +SLN+T + + ++
Sbjct: 70 HHAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKL 129
Query: 143 PVSQISDPLLLSAFADCIELLDDAVDAFSRSL-------FSTAASNH--DVLTWLSAALT 202
A DC+E +D+ +D +++ + S H D+ T +SAA+T
Sbjct: 130 LKRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMT 189
Query: 203 YHDTCTEGLADVSAGQ-VKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPVQNRRR 262
TC +G + A + V+D ++ + ++ SN+LA+ + + N R+
Sbjct: 190 NQGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNM--TDTDMMIMRTSNNRK 249
Query: 263 LMEAEWPQENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAIAAAPS 322
L+E + + + +P W+S DR+LL S S++ ++VVA DG+G +KTVA ++AAAP
Sbjct: 250 LIE----ETSTVDGWPAWLSTGDRRLLQS--SSVTPNVVVAADGSGNFKTVAASVAAAPQ 309
Query: 323 SSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFA 382
+R +I +KAG Y EN++V +K N+MF+GDG+ T+I+G ++V D TTF++AT A
Sbjct: 310 GGTKRYIIRIKAGVY-RENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVA 369
Query: 383 ASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQFYREC 442
G G + RD+TF+NTAGP +HQAVALRV AD + + C+++ YQDTLYVHSNRQF+ C
Sbjct: 370 VVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNC 429
Query: 443 DIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKILAAS 502
I GTVDFIFGNA V Q+C+I+ARKP GQKN +TAQ R DPNQNTGI I +I A S
Sbjct: 430 LIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATS 489
Query: 503 DLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSG 562
DL+ +GS+PT+LGRPWK YSR V M SS+ D IHP GW EW G+FAL+TL+YGE+ N+G
Sbjct: 490 DLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAG 549
Query: 563 PGAAVGQRVKWPGYRVITSTIEAGKFTVGQFIYGSAWLPSTGVSFSAGL 599
GA RVKW G+RVITS EA FT G FI GS+WL STG FS GL
Sbjct: 550 AGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584
BLAST of MS006200 vs. ExPASy Swiss-Prot
Match:
Q43143 (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PMEU1 PE=2 SV=1)
HSP 1 Score: 437.2 bits (1123), Expect = 3.0e-121
Identity = 248/578 (42.91%), Postives = 357/578 (61.76%), Query Frame = 0
Query: 39 RTRVVLLSLLSILLITASAISAVVFVRSHIGRSGESG--LGLAHKPSQAISRACSRTLYP 98
R + + L++++ +L+ A+ I V V+SH S + + ++ + ACS TL+P
Sbjct: 17 RKKKIYLAIVASVLLVAAVIGVVAGVKSHSKNSDDHADIMAISSSAHAIVKSACSNTLHP 76
Query: 99 AVCVSSLV---EFPGALTADVHDLVHISLNVTLQRLTKALYSSSQ-IPVSQISDPLLLSA 158
+C S++V +F +T+ D++ +SLN+T++ + + Y+ + I + P A
Sbjct: 77 ELCYSAIVNVSDFSKKVTSQ-KDVIELSLNITVKAVRRNYYAVKELIKTRKGLTPREKVA 136
Query: 159 FADCIELLDDAVDAFSRSL---------FSTAASNHDVLTWLSAALTYHDTCTEGLA-DV 218
DC+E +D+ +D ++ S D+ T +S+A+T +TC +G + D
Sbjct: 137 LHDCLETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLISSAITNQETCLDGFSHDE 196
Query: 219 SAGQVKDQMTAKLADLSQLISNSLAIFSGFGGG--TGEFSGVPVQNRRRLMEAEWPQENG 278
+ +V+ + + ++ SN+LA+ E N R+L+E +NG
Sbjct: 197 ADKKVRKVLLKGQKHVEKMCSNALAMICNMTDTDIANEMKLSAPANNRKLVE-----DNG 256
Query: 279 GEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAIAAAPSSSGRRTVIYVK 338
E+PEW+S DR+LL S S + D+VVA DG+G YKTV+EA+ AP S +R VI +K
Sbjct: 257 --EWPEWLSAGDRRLLQS--STVTPDVVVAADGSGDYKTVSEAVRKAPEKSSKRYVIRIK 316
Query: 339 AGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAASGTGIILRDM 398
AG Y EN+ V +KKTN+MF+GDGK T+I+ ++V D TTF +AT ++ RD+
Sbjct: 317 AGVY-RENVDVPKKKTNIMFMGDGKSNTIITASRNVQDGSTTFHSATVVRVAGKVLARDI 376
Query: 399 TFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQFYRECDIYGTVDFIFG 458
TF+NTAG +HQAVAL V +D + +RC+++ YQDTLYVHSNRQF+ +C + GTVDFIFG
Sbjct: 377 TFQNTAGASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVHSNRQFFVQCLVAGTVDFIFG 436
Query: 459 NAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKILAASDLEGSKGSYPT 518
N VFQ C+I+AR+P GQKN +TAQ R DPNQNTGI I C+I A SDL + S+PT
Sbjct: 437 NGAAVFQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRPVQKSFPT 496
Query: 519 FLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRVKW 578
+LGRPWK YSR V M SS+ D I P GW EW G+FALDTL+YGEY N+G GA RVKW
Sbjct: 497 YLGRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDTLFYGEYANTGAGAPTSGRVKW 556
Query: 579 PGYRVITSTIEAGKFTVGQFIYGSAWLPSTGVSFSAGL 599
G++VITS+ EA +T G+FI G +WL STG FS GL
Sbjct: 557 KGHKVITSSTEAQAYTPGRFIAGGSWLSSTGFPFSLGL 583
BLAST of MS006200 vs. ExPASy TrEMBL
Match:
A0A6J1DY95 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111024627 PE=3 SV=1)
HSP 1 Score: 1163.3 bits (3008), Expect = 0.0e+00
Identity = 592/597 (99.16%), Postives = 594/597 (99.50%), Query Frame = 0
Query: 5 MNYGKLHSTSSGETPANFELSNSNSSISSPNRRTRTRVVLLSLLSILLITASAISAVVFV 64
MNYGKLHSTSSG+TPANFELSNSNSSISSPNRRTRTRVVLLSLLSILLI ASAISAVVFV
Sbjct: 1 MNYGKLHSTSSGDTPANFELSNSNSSISSPNRRTRTRVVLLSLLSILLIMASAISAVVFV 60
Query: 65 RSHIGRSGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALTADVHDLVHISLNV 124
RSHIGR GESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGAL ADVHDLVHISLNV
Sbjct: 61 RSHIGRGGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALAADVHDLVHISLNV 120
Query: 125 TLQRLTKALYSSSQIPVSQISDPLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDVLTW 184
TLQRLTKALYSSSQIPVSQISDPLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDVLTW
Sbjct: 121 TLQRLTKALYSSSQIPVSQISDPLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDVLTW 180
Query: 185 LSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPV 244
LSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPV
Sbjct: 181 LSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPV 240
Query: 245 QNRRRLMEAEWPQENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAI 304
QNRRRLMEAEWPQENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAI
Sbjct: 241 QNRRRLMEAEWPQENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAI 300
Query: 305 AAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFR 364
AAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFR
Sbjct: 301 AAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFR 360
Query: 365 TATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQ 424
TATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQ
Sbjct: 361 TATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQ 420
Query: 425 FYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACK 484
FYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACK
Sbjct: 421 FYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACK 480
Query: 485 ILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGE 544
ILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGE
Sbjct: 481 ILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGE 540
Query: 545 YMNSGPGAAVGQRVKWPGYRVITSTIEAGKFTVGQFIYGSAWLPSTGVSFSAGLSAS 602
YMNSGPGAAVGQRVKWPGYRVITST+EAGKFTVGQFIYGSAWLPSTGVSFSAGLSAS
Sbjct: 541 YMNSGPGAAVGQRVKWPGYRVITSTVEAGKFTVGQFIYGSAWLPSTGVSFSAGLSAS 597
BLAST of MS006200 vs. ExPASy TrEMBL
Match:
A0A6J1H2K1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459120 PE=3 SV=1)
HSP 1 Score: 921.8 bits (2381), Expect = 1.5e-264
Identity = 478/603 (79.27%), Postives = 530/603 (87.89%), Query Frame = 0
Query: 6 NYGKLHSTSSGETPANFELSNSNSSISSPNRRTRTRVVLLSLLSILLITASAISAVVFVR 65
NY KLHS++SG+TPA F++S++ + SS R RTR++ +SL+SILLITASA+SA V +
Sbjct: 3 NYNKLHSSASGDTPATFQVSHTPNPPSSSASRRRTRILFVSLISILLITASAVSAAVLLH 62
Query: 66 SHIGRSGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALTADVHDLVHISLNVT 125
S +S +KP+QAIS ACS TLYP++C SSL+ FPG+L+ADVH LVHISLN+T
Sbjct: 63 S------QSSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISLNLT 122
Query: 126 LQRLTKALYSSSQIPVSQIS-DPLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDVLTW 185
LQ LTKALY+SSQIPV QIS +PL SA+ DCIELL+DAVDAFS SLFST+ASNHD++TW
Sbjct: 123 LQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMTW 182
Query: 186 LSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPV 245
LSAALTYHDTC++G ADV VKDQM A+L+DLS++ISNSLAIFSG+G G+PV
Sbjct: 183 LSAALTYHDTCSDGFADVD-DPVKDQMAARLSDLSEMISNSLAIFSGYG------EGLPV 242
Query: 246 QNRRRLMEA--EWPQENG----GEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYK 305
QNRRRLME+ EW ENG EFP WVS KDR+LL +PISAIQADIVVAKDG+GK+K
Sbjct: 243 QNRRRLMESDREWAAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFK 302
Query: 306 TVAEAIAAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFD 365
TVAEAI AAPSSSGRR VIY+KAGKY EENLKVGRKKTNLMFVGDGKGITVISGGKSVFD
Sbjct: 303 TVAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFD 362
Query: 366 KVTTFRTATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLY 425
KVTTFRTATFA SGTGIILRDMTFENTAGP +HQAVALRVSADHAVV+RCNIIGYQDTLY
Sbjct: 363 KVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRVSADHAVVYRCNIIGYQDTLY 422
Query: 426 VHSNRQFYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGI 485
VHSNRQF+RECDIYGTVDFIFGNAVVVFQSCNIYARKPMP QKNTITAQ+RKDPNQNTGI
Sbjct: 423 VHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQNTGI 482
Query: 486 SIHACKILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD 545
SIHACKILA +DLE SKGS+PTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD
Sbjct: 483 SIHACKILATTDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD 542
Query: 546 TLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEAGKFTVGQFIYGSAWLPSTGVSFSAGL 602
TLYYGEYMNSGPGAA GQRV WPGYRVITSTIEA KFTVGQFIYGS+WLPSTGVSFSAGL
Sbjct: 543 TLYYGEYMNSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFSAGL 592
BLAST of MS006200 vs. ExPASy TrEMBL
Match:
A0A6J1K6C1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111491552 PE=3 SV=1)
HSP 1 Score: 911.4 bits (2354), Expect = 2.0e-261
Identity = 475/606 (78.38%), Postives = 523/606 (86.30%), Query Frame = 0
Query: 6 NYGKLHSTSSGETPANFELS---NSNSSISSPNRRTRTRVVLLSLLSILLITASAISAVV 65
NY KLHS+ SG+TPA F++S N SS R RTR++ +SL+SILLITASA+SA V
Sbjct: 3 NYNKLHSSGSGDTPATFQVSHTPNPPHPPSSSASRRRTRILFVSLISILLITASAVSAAV 62
Query: 66 FVRSHIGRSGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALTADVHDLVHISL 125
+ SH +KP+QAIS ACSRTLYP++C SSL+ FPG+L+ADVH LVHISL
Sbjct: 63 LIHSHSSHIPH------NKPTQAISHACSRTLYPSLCRSSLLSFPGSLSADVHHLVHISL 122
Query: 126 NVTLQRLTKALYSSSQIPVSQIS-DPLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDV 185
N+TLQ LTKALY+SSQIPV QIS +PL SA+ DCIELL+DAVDAFS SLFST+ASNHD+
Sbjct: 123 NLTLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDI 182
Query: 186 LTWLSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSG 245
+TWLSAALTYHDTC++G ADV VKDQM ++L+DLS++ISNSLAIFSG+G G
Sbjct: 183 MTWLSAALTYHDTCSDGFADVD-DPVKDQMASRLSDLSEMISNSLAIFSGYG------EG 242
Query: 246 VPVQNRRRLMEA--EWPQENG----GEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTG 305
+PVQNRRRLME+ EW ENG EFP WVS KDR+LL + ISAIQADIVVAKDG+G
Sbjct: 243 LPVQNRRRLMESDREWAAENGVAVAEGEFPGWVSGKDRRLLAASISAIQADIVVAKDGSG 302
Query: 306 KYKTVAEAIAAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKS 365
K+KTVAEAI AAPSSSGRR VIY+KAGKY EENLKVGRKKTNLMFVGDGKGITVISGGKS
Sbjct: 303 KFKTVAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKS 362
Query: 366 VFDKVTTFRTATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQD 425
VFDKVTTFRTATFA SGTGIILRDMT ENTAGP +HQAVALRVSADHAVV+RCNIIGYQD
Sbjct: 363 VFDKVTTFRTATFAGSGTGIILRDMTIENTAGPSKHQAVALRVSADHAVVYRCNIIGYQD 422
Query: 426 TLYVHSNRQFYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQN 485
TLYVHSNRQF+RECDIYGTVDFIFGNAVVVFQSCNIYARKPMP QKNTITAQ+RKDPNQN
Sbjct: 423 TLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQN 482
Query: 486 TGISIHACKILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF 545
TGISIHACKILA +DLE SKGS+ TFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF
Sbjct: 483 TGISIHACKILATTDLEASKGSFSTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF 542
Query: 546 ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEAGKFTVGQFIYGSAWLPSTGVSFS 602
ALDTLYYGEYMNSGPGAA GQRV WPGYRVITSTIEA KFTVGQFIYGS+WLPSTGVSFS
Sbjct: 543 ALDTLYYGEYMNSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFS 595
BLAST of MS006200 vs. ExPASy TrEMBL
Match:
A0A6J1E2V1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430323 PE=3 SV=1)
HSP 1 Score: 909.4 bits (2349), Expect = 7.7e-261
Identity = 472/606 (77.89%), Postives = 531/606 (87.62%), Query Frame = 0
Query: 6 NYGKLHSTSSGETPA-NFELSNSNSSISSP--NRRTRTRVVLLSLLSILLITASAISAVV 65
NY LHS+ SG+TPA +F +SN +S P +R R++L+S SILLITASA+SAVV
Sbjct: 4 NYNMLHSSHSGDTPASSFRVSNDHSDPLPPPSPSSSRRRILLISFFSILLITASAVSAVV 63
Query: 66 FVRSHIGRSGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALTADVHDLVHISL 125
V S I S ES +KP+QAISRACS TLYP++C+SSL+ PG+L+ADVHDLVHISL
Sbjct: 64 LVHSRIAASTESHFP-HNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDLVHISL 123
Query: 126 NVTLQRLTKALYSSSQIPVSQIS-DPLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDV 185
N+TLQ LTKALYS+SQIP+SQIS DPL SA+ DC+ELL+DAV+AFS SLFST A+NHDV
Sbjct: 124 NLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTATNHDV 183
Query: 186 LTWLSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSG 245
+TWLSAALTYHDTCT+G +DV A +KDQ+TAKL+DLS++ISNSLAIFSGFGG
Sbjct: 184 MTWLSAALTYHDTCTDGFSDV-ADPIKDQITAKLSDLSEMISNSLAIFSGFGG-----DD 243
Query: 246 VPVQN--RRRLMEA--EW--PQENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTG 305
+PV+N RRRLME+ +W + NG +FP WVS KDR+LL +PIS+IQADIVVAKDG+G
Sbjct: 244 LPVENRRRRRLMESDEQWLAEENNGDGDFPRWVSGKDRRLLAAPISSIQADIVVAKDGSG 303
Query: 306 KYKTVAEAIAAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKS 365
K+KTVAEAIAAAPSSSGRR VIY+KAGKY EENLKVGRKKTNLMFVGDGKGITVISGGKS
Sbjct: 304 KFKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKS 363
Query: 366 VFDKVTTFRTATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQD 425
V+DKVTTFRTATFA SGTG+ILRDMTFENTAGP +HQAVALR+SADHAVVFR N+IGYQD
Sbjct: 364 VYDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVIGYQD 423
Query: 426 TLYVHSNRQFYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQN 485
TLYVHSNRQF+RECDIYGT+DFIFGNAVVVFQSCNIYARKPM GQKNTITAQDRKDPNQN
Sbjct: 424 TLYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMSGQKNTITAQDRKDPNQN 483
Query: 486 TGISIHACKILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF 545
TGISIHACKI+A DL+ SKGS+PT+LGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF
Sbjct: 484 TGISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF 543
Query: 546 ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEAGKFTVGQFIYGSAWLPSTGVSFS 602
ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITS IEA KFTVGQFIYGS+WLPSTGVSFS
Sbjct: 544 ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSAIEATKFTVGQFIYGSSWLPSTGVSFS 602
BLAST of MS006200 vs. ExPASy TrEMBL
Match:
A0A6J1IA52 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111471502 PE=3 SV=1)
HSP 1 Score: 901.7 bits (2329), Expect = 1.6e-258
Identity = 468/606 (77.23%), Postives = 526/606 (86.80%), Query Frame = 0
Query: 6 NYGKLHSTSSGETP-ANFELSN--SNSSISSPNRRTRTRVVLLSLLSILLITASAISAVV 65
NY KLHS+ SG+TP +F +SN SN P + R++L+S SILLITAS +SAVV
Sbjct: 4 NYNKLHSSRSGDTPVTSFRVSNDHSNPPPPPPPSSSHRRILLISFFSILLITASTVSAVV 63
Query: 66 FVRSHIGRSGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALTADVHDLVHISL 125
V S I S ES +KP+QAISRACS TLYP++C+SSL+ PG+L+ADVHDLVHISL
Sbjct: 64 LVHSRITTSTESHFP-HNKPTQAISRACSLTLYPSLCISSLLSAPGSLSADVHDLVHISL 123
Query: 126 NVTLQRLTKALYSSSQIPVSQIS-DPLLLSAFADCIELLDDAVDAFSRSLFSTAASNHDV 185
N+TLQ LTKALYS+SQIP+SQIS DPL SA+ DC+ELL+DAV+AFS SLFST A+NHDV
Sbjct: 124 NLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTATNHDV 183
Query: 186 LTWLSAALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSG 245
+TWLSAALTYHDTCT+G +DV A VKDQ+TAKL+DLS++ISNSLAIFSGFGGG
Sbjct: 184 MTWLSAALTYHDTCTDGFSDV-ADPVKDQITAKLSDLSEMISNSLAIFSGFGGG-----D 243
Query: 246 VPVQN--RRRLMEA--EW--PQENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTG 305
+PV+N RRRLME+ +W + NG +FP WVS KDR+LL +PIS+IQADIVVAKDG+G
Sbjct: 244 LPVENRRRRRLMESDEQWLAEENNGDGDFPRWVSGKDRRLLAAPISSIQADIVVAKDGSG 303
Query: 306 KYKTVAEAIAAAPSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKS 365
K+KTVAEAIAAAPSSS RR VIY+KAGKY EENLKVGRKKTNLMFVGDGKGITVISGGKS
Sbjct: 304 KFKTVAEAIAAAPSSSSRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKS 363
Query: 366 VFDKVTTFRTATFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQD 425
V+DKVTTFRTATFA SGTG+ILRDMTFENTAGP +HQAVALR+SADH VVFR N+IGYQD
Sbjct: 364 VYDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHTVVFRSNVIGYQD 423
Query: 426 TLYVHSNRQFYRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQN 485
TLYVHSNRQF+RECDIYGT+DFIFGNA VVFQSCNIYARKPM GQKNTITAQDRKDPNQN
Sbjct: 424 TLYVHSNRQFFRECDIYGTIDFIFGNAAVVFQSCNIYARKPMSGQKNTITAQDRKDPNQN 483
Query: 486 TGISIHACKILAASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF 545
TGISIHACKI+A DL+ SKGS+PT+LGRPWKLYSRVVYMVSS+GDHIHPRGWLEWQGSF
Sbjct: 484 TGISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSLGDHIHPRGWLEWQGSF 543
Query: 546 ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEAGKFTVGQFIYGSAWLPSTGVSFS 602
ALDTLYYGEYMNSGP AAVGQRVKWPGYRVITS IEA KFTVGQFIYGS+WLPSTGVSFS
Sbjct: 544 ALDTLYYGEYMNSGPSAAVGQRVKWPGYRVITSAIEATKFTVGQFIYGSSWLPSTGVSFS 602
BLAST of MS006200 vs. TAIR 10
Match:
AT5G53370.1 (pectin methylesterase PCR fragment F )
HSP 1 Score: 726.1 bits (1873), Expect = 2.3e-209
Identity = 366/588 (62.24%), Postives = 453/588 (77.04%), Query Frame = 0
Query: 19 PANFELSNSNSSISSPNRRTRTRVVLLSLLSILLITASAISAVVFVRSHIGRSGESGLGL 78
P+N + +S+ ++T+T+++L +L ++ + +F SG++ L
Sbjct: 11 PSNPNQKDPATSLPELQKKTKTKLILFTL---AVLVVGVVCFGIFAGIRAVDSGKTEPKL 70
Query: 79 AHKPSQAISRACSRTLYPAVCVSSLVEFPGALTADVHDLVHISLNVTLQRLTKALYSSSQ 138
KP+QAISR CS++LYP +C+ +L++FPG+LTAD ++L+HIS N TLQ+ +KALY+SS
Sbjct: 71 TRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSST 130
Query: 139 IPVSQISDPLLLSAFADCIELLDDAVDAFSRSLFSTAA-----SNHDVLTWLSAALTYHD 198
I +Q+ P + SA+ C+ELLDD+VDA +R+L S S+ DV+TWLS+A+T HD
Sbjct: 131 ITYTQM-PPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTNHD 190
Query: 199 TCTEGLADV--SAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPVQNRRRLM 258
TCT+G ++ G+VKDQ+ + DLS+++SN LAIF+ G + SGVPV N R+L+
Sbjct: 191 TCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFA---GKVKDLSGVPVVNNRKLL 250
Query: 259 EAEWPQENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAIAAAPSSS 318
E EE P W+ R+DR+LLG+P SAIQADI V+KDG+G +KT+AEAI AP S
Sbjct: 251 GTE-----ETEELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHS 310
Query: 319 GRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAAS 378
RR VIYVKAG+Y EENLKVGRKKTNLMF+GDGKG TVI+GGKS+ D +TTF TATFAA+
Sbjct: 311 SRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAAT 370
Query: 379 GTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQFYRECDI 438
G G I+RDMTFEN AGP +HQAVALRV DHAVV+RCNIIGYQD LYVHSNRQF+REC+I
Sbjct: 371 GAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEI 430
Query: 439 YGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKILAASDL 498
YGTVDFIFGNA V+ QSCNIYARKPM QK TITAQ+RKDPNQNTGISIHACK+LA DL
Sbjct: 431 YGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDL 490
Query: 499 EGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPG 558
E SKGSYPT+LGRPWKLYSRVVYM+S MGDHI PRGWLEW G FALD+LYYGEYMN G G
Sbjct: 491 EASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLG 550
Query: 559 AAVGQRVKWPGYRVITSTIEAGKFTVGQFIYGSAWLPSTGVSFSAGLS 600
+ +GQRVKWPGY VITST+EA KFTV QFI GS+WLPSTGVSF +GLS
Sbjct: 551 SGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586
BLAST of MS006200 vs. TAIR 10
Match:
AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )
HSP 1 Score: 699.5 bits (1804), Expect = 2.3e-201
Identity = 368/593 (62.06%), Postives = 453/593 (76.39%), Query Frame = 0
Query: 12 STSSGETPANFELSNSNSSISSPNRRTRTRVVLLSL-LSILLITASAISAVVFVRSHIGR 71
+TS+ P ++S S + NRR++ ++V+ S+ L+I LI A+AI A VRS + +
Sbjct: 16 TTSTTTAPILNQVSTSEQP-ENNNRRSKKKLVVSSIVLAISLILAAAIFA--GVRSRL-K 75
Query: 72 SGESGLGLAHKPSQAISRACSRTLYPAVCVSSLVEFPGALTA-DVHDLVHISLNVTLQRL 131
+S GLA KPSQAIS+AC T +P +CV SL++FPG+L A DL+H+++N+TL
Sbjct: 76 LNQSVPGLARKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHF 135
Query: 132 TKALYSSSQIPVSQISDPLLLSAFADCIELLDDAVDAFSRSLFSTAASN---HDVLTWLS 191
+ ALYSS+ + + P SA+ C+ELLDD+VDA SR+L S +S+ DV TWLS
Sbjct: 136 SHALYSSASLSFVDM-PPRARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLS 195
Query: 192 AALTYHDTCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPVQN 251
AALT HDTCTEG V G VKD MTA L +LS+L+SN LAIFS G +F+GVP+QN
Sbjct: 196 AALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDG-DDFAGVPIQN 255
Query: 252 RRRLMEAEWPQENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAIAA 311
RR L E E+FP W+ K+R++L P+S IQADI+V+KDG G KT++EAI
Sbjct: 256 RRLL-----GVEEREEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKK 315
Query: 312 APSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTA 371
AP +S RR +IYVKAG+Y E NLKVGRKK NLMFVGDGKG TVISGGKS+FD +TTF TA
Sbjct: 316 APQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTA 375
Query: 372 TFAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQFY 431
+FAA+G G I RD+TFEN AGP +HQAVALR+ ADHAV++RCNIIGYQDTLYVHSNRQF+
Sbjct: 376 SFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFF 435
Query: 432 RECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKIL 491
RECDIYGTVDFIFGNA VV Q+C+IYARKPM QKNTITAQ+RKDPNQNTGISIHA ++L
Sbjct: 436 RECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVL 495
Query: 492 AASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYM 551
AASDL+ + GS T+LGRPWKL+SR VYM+S +G H+H RGWLEW +FALDTLYYGEY+
Sbjct: 496 AASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTLYYGEYL 555
Query: 552 NSGPGAAVGQRVKWPGYRVITSTIEAGKFTVGQFIYGSAWLPSTGVSFSAGLS 600
NSGPG+ +GQRV WPGYRVI ST EA +FTV +FIYGS+WLPSTGVSF AGLS
Sbjct: 556 NSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 597
BLAST of MS006200 vs. TAIR 10
Match:
AT3G14310.1 (pectin methylesterase 3 )
HSP 1 Score: 433.3 bits (1113), Expect = 3.1e-121
Identity = 251/591 (42.47%), Postives = 353/591 (59.73%), Query Frame = 0
Query: 32 SSPNRRTRTRVVLLSLLSILLITASAISAVVFVRSHIGRSGESGLGLAHKPSQAISRACS 91
S N + ++VLLS LL ++AV + + ++ E L+ + +CS
Sbjct: 10 SKDNFKKNKKLVLLSAAVALLF----VAAVAGISAGASKANEKRT-LSPSSHAVLRSSCS 69
Query: 92 RTLYPAVCVSSLVEFPGALTADVHDLVHISLNVTLQRLTKALYSSSQ-IPVSQISDPLLL 151
T YP +C+S++V G D++ S+N+T+ + ++ + I + P
Sbjct: 70 STRYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREK 129
Query: 152 SAFADCIELLDDAVDAFSRS-----LFSTAAS--NH--DVLTWLSAALTYHDTCTEGLA- 211
+A DC+E +D+ +D + L+ T + H D+ T +S+A+T +TC +G +
Sbjct: 130 TALHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSH 189
Query: 212 DVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGG---GTGEFSGVPVQNRRRLMEAEWPQ 271
D + QV+ + + + SN+LA+ E N R+L E
Sbjct: 190 DDADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQET 249
Query: 272 ----------ENGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAIAAA 331
E E +P W+S DR+LL S ++AD VA DG+G +KTVA A+AAA
Sbjct: 250 TVAVDIAGAGELDSEGWPTWLSAGDRRLLQG--SGVKADATVAADGSGTFKTVAAAVAAA 309
Query: 332 PSSSGRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTAT 391
P +S +R VI++KAG Y EN++V +KK N+MF+GDG+ T+I+G ++V D TTF +AT
Sbjct: 310 PENSNKRYVIHIKAGVY-RENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSAT 369
Query: 392 FAASGTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQFYR 451
AA G + RD+TF+NTAGP +HQAVALRV +D + + C+++ YQDTLYVHSNRQF+
Sbjct: 370 VAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFV 429
Query: 452 ECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKILA 511
+C I GTVDFIFGNA VV Q C+I+AR+P GQKN +TAQ R DPNQNTGI I C+I A
Sbjct: 430 KCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGA 489
Query: 512 ASDLEGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMN 571
SDL+ KGS+PT+LGRPWK YS+ V M S++ D I P GW EW G+FAL+TL Y EY N
Sbjct: 490 TSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSN 549
Query: 572 SGPGAAVGQRVKWPGYRVITSTIEAGKFTVGQFIYGSAWLPSTGVSFSAGL 599
+G GA RVKW G++VIT+ EA K+T GQFI G WL STG FS GL
Sbjct: 550 TGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592
BLAST of MS006200 vs. TAIR 10
Match:
AT1G11580.1 (methylesterase PCR A )
HSP 1 Score: 429.5 bits (1103), Expect = 4.5e-120
Identity = 257/587 (43.78%), Postives = 349/587 (59.45%), Query Frame = 0
Query: 24 LSNSNSSISSPNRRTRTRVVLLSLLSILLITASAISAVVFVRSHIGRSGESGLGLAHKPS 83
+SNSN + S + + + + L L + ++ + A + + + +S L
Sbjct: 1 MSNSNQPLLSKPKSLKHKNLCLVLSFVAILGSVAFFTAQLISVNTNNNDDSLL------- 60
Query: 84 QAISRACSRTLYPAVCVSSLVEFPGALTADVH--DLVHISLNVTLQRL--TKALYSSSQI 143
S+ C C + L EF + ++ DL+H+ L ++ RL T + S ++I
Sbjct: 61 -TTSQICHGAHDQDSCQALLSEFTTLSLSKLNRLDLLHVFLKNSVWRLESTMTMVSEARI 120
Query: 144 PVSQISDPLLLSAFADCIELLDDAVDAFSRSLFSTAASNH------DVLTWLSAALTYHD 203
+ + D + FADC E++D + D S+ N+ +V TWLS+ LT +
Sbjct: 121 RSNGVRDK---AGFADCEEMMDVSKDRMMSSMEELRGGNYNLESYSNVHTWLSSVLTNYM 180
Query: 204 TCTEGLADVSAGQVKDQMTAKLADLSQLISNSLAIFSGFGGGTGEFSGVPVQNRRRLMEA 263
TC E ++DVS K + +L DL +LAIF S +P ++ +++ +
Sbjct: 181 TCLESISDVSVNS-KQIVKPQLEDLVSRARVALAIF---------VSVLPARDDLKMIIS 240
Query: 264 EWPQENGGEEFPEWVSRKDRQLLGSPISAIQ--ADIVVAKDGTGKYKTVAEAIAAAPSSS 323
FP W++ DR+LL S ++ A++VVAKDGTGK+KTV EA+AAAP +S
Sbjct: 241 --------NRFPSWLTALDRKLLESSPKTLKVTANVVVAKDGTGKFKTVNEAVAAAPENS 300
Query: 324 GRRTVIYVKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAAS 383
R VIYVK G Y +E + +G+KK NLM VGDGK T+I+G +V D TTFR+AT AA+
Sbjct: 301 NTRYVIYVKKGVY-KETIDIGKKKKNLMLVGDGKDATIITGSLNVIDGSTTFRSATVAAN 360
Query: 384 GTGIILRDMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQFYRECDI 443
G G + +D+ F+NTAGP +HQAVALRVSAD V+ RC I YQDTLY H+ RQFYR+ I
Sbjct: 361 GDGFMAQDIWFQNTAGPAKHQAVALRVSADQTVINRCRIDAYQDTLYTHTLRQFYRDSYI 420
Query: 444 YGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKILAASDL 503
GTVDFIFGN+ VVFQ+C+I AR P GQKN +TAQ R+D NQNT ISI CKI A+SDL
Sbjct: 421 TGTVDFIFGNSAVVFQNCDIVARNPGAGQKNMLTAQGREDQNQNTAISIQKCKITASSDL 480
Query: 504 EGSKGSYPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPG 563
KGS TFLGRPWKLYSR V M S + +HI P GW W G FAL TLYYGEY N+GPG
Sbjct: 481 APVKGSVKTFLGRPWKLYSRTVIMQSFIDNHIDPAGWFPWDGEFALSTLYYGEYANTGPG 540
Query: 564 AAVGQRVKWPGYRVITSTIEAGKFTVGQFIYGSAWLPSTGVSFSAGL 599
A +RV W G++VI + EA +FTV + I G WL TGV+F L
Sbjct: 541 ADTSKRVNWKGFKVIKDSKEAEQFTVAKLIQGGLWLKPTGVTFQEWL 557
BLAST of MS006200 vs. TAIR 10
Match:
AT1G53830.1 (pectin methylesterase 2 )
HSP 1 Score: 426.8 bits (1096), Expect = 2.9e-119
Identity = 244/580 (42.07%), Postives = 338/580 (58.28%), Query Frame = 0
Query: 37 RTRTRVVLLSLLSILLITASAISAVVFVRSHIGRSGESGLGLAHKPSQAISRACSRTLYP 96
+ +++L S LL+ AS + + + L+ + CS TLYP
Sbjct: 15 KNNKKLILSSAAIALLLLASIVGIAATTTNQ--NKNQKITTLSSTSHAILKSVCSSTLYP 74
Query: 97 AVCVSSLVEFPGALTADVHDLVHISLNVTLQRLTKALYSSSQIPVSQIS-DPLLLSAFAD 156
+C S++ G +++ SLN+T + + ++ ++ + P ++A D
Sbjct: 75 ELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHD 134
Query: 157 CIELLDDAVDAFSRSL---------FSTAASNHDVLTWLSAALTYHDTCTEGLA-DVSAG 216
C+E +D+ +D ++ S D+ T +S+A+T TC +G + D +
Sbjct: 135 CLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADR 194
Query: 217 QVKDQMTAKLADLSQLISNSLAIFSGFGGG-------TGEFSGVPVQNRRRLMEAEWPQE 276
+V+ + + + SN+LA+ + S N R+L E +
Sbjct: 195 KVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGDLD 254
Query: 277 NGGEEFPEWVSRKDRQLLGSPISAIQADIVVAKDGTGKYKTVAEAIAAAPSSSGRRTVIY 336
+ G +P+W+S DR+LL S I+AD VA DG+G + TVA A+AAAP S +R VI+
Sbjct: 255 SDG--WPKWLSVGDRRLLQG--STIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIH 314
Query: 337 VKAGKYVEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDKVTTFRTATFAASGTGIILR 396
+KAG Y EN++V +KKTN+MF+GDG+G T+I+G ++V D TTF +AT AA G + R
Sbjct: 315 IKAGVY-RENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLAR 374
Query: 397 DMTFENTAGPGRHQAVALRVSADHAVVFRCNIIGYQDTLYVHSNRQFYRECDIYGTVDFI 456
D+TF+NTAGP +HQAVALRV +D + ++C++ YQDTLYVHSNRQF+ +C I GTVDFI
Sbjct: 375 DITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFI 434
Query: 457 FGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTGISIHACKILAASDLEGSKGSY 516
FGNA V Q C+I AR+P GQKN +TAQ R DPNQNTGI I C+I SDL KG++
Sbjct: 435 FGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTF 494
Query: 517 PTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRV 576
PT+LGRPWK YSR V M S + D I P GW EW GSFALDTL Y EY+N G GA RV
Sbjct: 495 PTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRV 554
Query: 577 KWPGYRVITSTIEAGKFTVGQFIYGSAWLPSTGVSFSAGL 599
KW GY+VITS EA FT GQFI G WL STG FS L
Sbjct: 555 KWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022158049.1 | 0.0e+00 | 99.16 | probable pectinesterase/pectinesterase inhibitor 61 [Momordica charantia] | [more] |
| XP_022957644.1 | 3.1e-264 | 79.27 | probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita moschata] | [more] |
| XP_023533011.1 | 3.4e-263 | 78.61 | probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita pepo subsp. pepo] | [more] |
| KAG6605784.1 | 2.9e-262 | 78.44 | putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosp... | [more] |
| KAG6579321.1 | 6.5e-262 | 78.22 | putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosp... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q9FK05 | 3.3e-208 | 62.24 | Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3... | [more] |
| Q9M3B0 | 3.3e-200 | 62.06 | Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3... | [more] |
| O04886 | 1.6e-122 | 43.63 | Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1 | [more] |
| P83948 | 2.1e-122 | 43.12 | Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1 | [more] |
| Q43143 | 3.0e-121 | 42.91 | Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PM... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1DY95 | 0.0e+00 | 99.16 | Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111024627 PE=3 SV=1 | [more] |
| A0A6J1H2K1 | 1.5e-264 | 79.27 | Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459120 PE=3 SV=1 | [more] |
| A0A6J1K6C1 | 2.0e-261 | 78.38 | Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111491552 PE=3 SV=1 | [more] |
| A0A6J1E2V1 | 7.7e-261 | 77.89 | Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430323 PE=3 SV=1 | [more] |
| A0A6J1IA52 | 1.6e-258 | 77.23 | Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111471502 PE=3 SV=1 | [more] |
