MS006193 (gene) Bitter gourd (TR) v1

BLAST of MS006193 vs. NCBI nr
Match: XP_022159305.1 (uncharacterized protein LOC111025715 isoform X2 [Momordica charantia] >XP_022159325.1 uncharacterized protein LOC111025733 isoform X2 [Momordica charantia])

HSP 1 Score: 1053.1 bits (2722), Expect = 9.1e-304
Identity = 562/617 (91.09%), Postives = 562/617 (91.09%), Query Frame = 0

Query: 1   RMSEICMCMQSLQLQKGIKFTCTSVCLQRQKCNVKLYAVPVRAFSCHSSSTPSASSRADL 60
           RMSEICMCMQSLQLQKGIKFTCTSVCLQRQKCNVKLYAVPVRAFSCHSSSTPSASSRADL
Sbjct: 16  RMSEICMCMQSLQLQKGIKFTCTSVCLQRQKCNVKLYAVPVRAFSCHSSSTPSASSRADL 75

Query: 61  RWGRRKHVPSTFTAPNGYQSTGHVHYRTDGNNISELESQLEELFNEVRMMIMSGRKNDAV 120
           RWGRRKHVPSTFTAPNGYQSTGHVHYRTD NNISELESQLEELFNEVRMMIMSGRKNDAV
Sbjct: 76  RWGRRKHVPSTFTAPNGYQSTGHVHYRTDENNISELESQLEELFNEVRMMIMSGRKNDAV 135

Query: 121 ELLEANYEAVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDNLKDG 180
           ELLEANYEAVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDNLKDG
Sbjct: 136 ELLEANYEAVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDNLKDG 195

Query: 181 EPFLDSVLLHMGSMYSTLKKFEKSMSVYKRAIDIIEKKSGKDSSLLITPILGMAKVIGTI 240
           EPFLDSVLLHMGSMYSTLKKFEKSMSVYKRAIDIIEKKSGKDSSLLITPILGMAKVIGTI
Sbjct: 196 EPFLDSVLLHMGSMYSTLKKFEKSMSVYKRAIDIIEKKSGKDSSLLITPILGMAKVIGTI 255

Query: 241 GRTSKAVQFYHRAISILESSRGFENEDLVIPLFNLGDLLLKEGKGKDAEACFARFSLSLS 300
           GRTSKAVQFYHRAISILESSRGFENEDLVIPLFNLGDLLLKEGKGKDAEACFA       
Sbjct: 256 GRTSKAVQFYHRAISILESSRGFENEDLVIPLFNLGDLLLKEGKGKDAEACFA------- 315

Query: 301 LSPSLLPLDYSLNLYALILRIVNIYKNLYGEKDGKVGMAMYSLANAKCARGEAEEAVILY 360
                              RIVNIYKNLYGEKDGKVGMAMYSLANAKCARGEAEEAVILY
Sbjct: 316 -------------------RIVNIYKNLYGEKDGKVGMAMYSLANAKCARGEAEEAVILY 375

Query: 361 RRALQIIKDSNYMALDDSVMEKMRIDLAELLHVVGRGNEGRELLEECLLINEKSKGKDDP 420
           RRALQIIKDSNYMALDDSVMEKMRIDLAELLHVVGRGNEGRELLEECLLINEKSKGKDDP
Sbjct: 376 RRALQIIKDSNYMALDDSVMEKMRIDLAELLHVVGRGNEGRELLEECLLINEKSKGKDDP 435

Query: 421 SSVKHLVNLASSYSRSKNYAEAERLLRMGLNIMVKAAGADDESITVPMLHLAVTLYNLNR 480
           SSVKHLVNLASSYSRSKNYAEAERLLRMGLNIMVKAAGADDESITVPMLHLAVTLYNLNR
Sbjct: 436 SSVKHLVNLASSYSRSKNYAEAERLLRMGLNIMVKAAGADDESITVPMLHLAVTLYNLNR 495

Query: 481 DEDAEQLALEALRIREIAFGKDCLPVGEKTENCLFLYLVGQSFSGDLICDVLDAGEALDC 540
           DEDAEQLALEALRIREIAFGKDCLPV                            GEALDC
Sbjct: 496 DEDAEQLALEALRIREIAFGKDCLPV----------------------------GEALDC 555

Query: 541 LVCIQSRVGKDEKELLNMLMRILRIQEKEFGMEGKEVIDTLKKIVFYMDKLGIKDEKFPL 600
           LVCIQSRVGKDEKELLNMLMRILRIQEKEFGMEGKEVIDTLKKIVFYMDKLGIKDEKFPL
Sbjct: 556 LVCIQSRVGKDEKELLNMLMRILRIQEKEFGMEGKEVIDTLKKIVFYMDKLGIKDEKFPL 578

Query: 601 QKRLSMLRMKFKNQMQY 618
           QKRLSMLRMKFKNQMQY
Sbjct: 616 QKRLSMLRMKFKNQMQY 578

BLAST of MS006193 vs. NCBI nr
Match: XP_022159303.1 (uncharacterized protein LOC111025715 isoform X1 [Momordica charantia] >XP_022159324.1 uncharacterized protein LOC111025733 isoform X1 [Momordica charantia])

HSP 1 Score: 1048.5 bits (2710), Expect = 2.3e-302
Identity = 562/618 (90.94%), Postives = 562/618 (90.94%), Query Frame = 0

Query: 1   RMSEICMCMQSLQLQKGIKFTCTSVCLQRQKCNVKLYAVPVRAFSCHSSSTPSASSRADL 60
           RMSEICMCMQSLQLQKGIKFTCTSVCLQRQKCNVKLYAVPVRAFSCHSSSTPSASSRADL
Sbjct: 16  RMSEICMCMQSLQLQKGIKFTCTSVCLQRQKCNVKLYAVPVRAFSCHSSSTPSASSRADL 75

Query: 61  RWGRRKHVPSTFTAPNGYQ-STGHVHYRTDGNNISELESQLEELFNEVRMMIMSGRKNDA 120
           RWGRRKHVPSTFTAPNGYQ STGHVHYRTD NNISELESQLEELFNEVRMMIMSGRKNDA
Sbjct: 76  RWGRRKHVPSTFTAPNGYQSSTGHVHYRTDENNISELESQLEELFNEVRMMIMSGRKNDA 135

Query: 121 VELLEANYEAVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDNLKD 180
           VELLEANYEAVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDNLKD
Sbjct: 136 VELLEANYEAVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDNLKD 195

Query: 181 GEPFLDSVLLHMGSMYSTLKKFEKSMSVYKRAIDIIEKKSGKDSSLLITPILGMAKVIGT 240
           GEPFLDSVLLHMGSMYSTLKKFEKSMSVYKRAIDIIEKKSGKDSSLLITPILGMAKVIGT
Sbjct: 196 GEPFLDSVLLHMGSMYSTLKKFEKSMSVYKRAIDIIEKKSGKDSSLLITPILGMAKVIGT 255

Query: 241 IGRTSKAVQFYHRAISILESSRGFENEDLVIPLFNLGDLLLKEGKGKDAEACFARFSLSL 300
           IGRTSKAVQFYHRAISILESSRGFENEDLVIPLFNLGDLLLKEGKGKDAEACFA      
Sbjct: 256 IGRTSKAVQFYHRAISILESSRGFENEDLVIPLFNLGDLLLKEGKGKDAEACFA------ 315

Query: 301 SLSPSLLPLDYSLNLYALILRIVNIYKNLYGEKDGKVGMAMYSLANAKCARGEAEEAVIL 360
                               RIVNIYKNLYGEKDGKVGMAMYSLANAKCARGEAEEAVIL
Sbjct: 316 --------------------RIVNIYKNLYGEKDGKVGMAMYSLANAKCARGEAEEAVIL 375

Query: 361 YRRALQIIKDSNYMALDDSVMEKMRIDLAELLHVVGRGNEGRELLEECLLINEKSKGKDD 420
           YRRALQIIKDSNYMALDDSVMEKMRIDLAELLHVVGRGNEGRELLEECLLINEKSKGKDD
Sbjct: 376 YRRALQIIKDSNYMALDDSVMEKMRIDLAELLHVVGRGNEGRELLEECLLINEKSKGKDD 435

Query: 421 PSSVKHLVNLASSYSRSKNYAEAERLLRMGLNIMVKAAGADDESITVPMLHLAVTLYNLN 480
           PSSVKHLVNLASSYSRSKNYAEAERLLRMGLNIMVKAAGADDESITVPMLHLAVTLYNLN
Sbjct: 436 PSSVKHLVNLASSYSRSKNYAEAERLLRMGLNIMVKAAGADDESITVPMLHLAVTLYNLN 495

Query: 481 RDEDAEQLALEALRIREIAFGKDCLPVGEKTENCLFLYLVGQSFSGDLICDVLDAGEALD 540
           RDEDAEQLALEALRIREIAFGKDCLPV                            GEALD
Sbjct: 496 RDEDAEQLALEALRIREIAFGKDCLPV----------------------------GEALD 555

Query: 541 CLVCIQSRVGKDEKELLNMLMRILRIQEKEFGMEGKEVIDTLKKIVFYMDKLGIKDEKFP 600
           CLVCIQSRVGKDEKELLNMLMRILRIQEKEFGMEGKEVIDTLKKIVFYMDKLGIKDEKFP
Sbjct: 556 CLVCIQSRVGKDEKELLNMLMRILRIQEKEFGMEGKEVIDTLKKIVFYMDKLGIKDEKFP 579

Query: 601 LQKRLSMLRMKFKNQMQY 618
           LQKRLSMLRMKFKNQMQY
Sbjct: 616 LQKRLSMLRMKFKNQMQY 579

BLAST of MS006193 vs. NCBI nr
Match: XP_022159306.1 (uncharacterized protein LOC111025715 isoform X3 [Momordica charantia] >XP_022159326.1 uncharacterized protein LOC111025733 isoform X3 [Momordica charantia])

HSP 1 Score: 1018.1 bits (2631), Expect = 3.3e-293
Identity = 550/618 (89.00%), Postives = 550/618 (89.00%), Query Frame = 0

Query: 1   RMSEICMCMQSLQLQKGIKFTCTSVCLQRQKCNVKLYAVPVRAFSCHSSSTPSASSRADL 60
           RMSEICMCMQSLQLQKGIKFTCTSVCLQRQKCNVKLYAVPVRAFSCHSSSTPSASSRADL
Sbjct: 16  RMSEICMCMQSLQLQKGIKFTCTSVCLQRQKCNVKLYAVPVRAFSCHSSSTPSASSRADL 75

Query: 61  RWGRRKHVPSTFTAPNGYQ-STGHVHYRTDGNNISELESQLEELFNEVRMMIMSGRKNDA 120
           RWGRRKHVPSTFTAPNGYQ STGHVHYRTD NNISELESQLEELFNEVRMMIMSGRKNDA
Sbjct: 76  RWGRRKHVPSTFTAPNGYQSSTGHVHYRTDENNISELESQLEELFNEVRMMIMSGRKNDA 135

Query: 121 VELLEANYEAVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDNLKD 180
           VELLEANYEAVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDNLKD
Sbjct: 136 VELLEANYEAVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDNLKD 195

Query: 181 GEPFLDSVLLHMGSMYSTLKKFEKSMSVYKRAIDIIEKKSGKDSSLLITPILGMAKVIGT 240
           GEPFLDSVLLHMGSMYSTLKKFEKSMSVYKRAIDIIEKKS            GMAKVIGT
Sbjct: 196 GEPFLDSVLLHMGSMYSTLKKFEKSMSVYKRAIDIIEKKS------------GMAKVIGT 255

Query: 241 IGRTSKAVQFYHRAISILESSRGFENEDLVIPLFNLGDLLLKEGKGKDAEACFARFSLSL 300
           IGRTSKAVQFYHRAISILESSRGFENEDLVIPLFNLGDLLLKEGKGKDAEACFA      
Sbjct: 256 IGRTSKAVQFYHRAISILESSRGFENEDLVIPLFNLGDLLLKEGKGKDAEACFA------ 315

Query: 301 SLSPSLLPLDYSLNLYALILRIVNIYKNLYGEKDGKVGMAMYSLANAKCARGEAEEAVIL 360
                               RIVNIYKNLYGEKDGKVGMAMYSLANAKCARGEAEEAVIL
Sbjct: 316 --------------------RIVNIYKNLYGEKDGKVGMAMYSLANAKCARGEAEEAVIL 375

Query: 361 YRRALQIIKDSNYMALDDSVMEKMRIDLAELLHVVGRGNEGRELLEECLLINEKSKGKDD 420
           YRRALQIIKDSNYMALDDSVMEKMRIDLAELLHVVGRGNEGRELLEECLLINEKSKGKDD
Sbjct: 376 YRRALQIIKDSNYMALDDSVMEKMRIDLAELLHVVGRGNEGRELLEECLLINEKSKGKDD 435

Query: 421 PSSVKHLVNLASSYSRSKNYAEAERLLRMGLNIMVKAAGADDESITVPMLHLAVTLYNLN 480
           PSSVKHLVNLASSYSRSKNYAEAERLLRMGLNIMVKAAGADDESITVPMLHLAVTLYNLN
Sbjct: 436 PSSVKHLVNLASSYSRSKNYAEAERLLRMGLNIMVKAAGADDESITVPMLHLAVTLYNLN 495

Query: 481 RDEDAEQLALEALRIREIAFGKDCLPVGEKTENCLFLYLVGQSFSGDLICDVLDAGEALD 540
           RDEDAEQLALEALRIREIAFGKDCLPV                            GEALD
Sbjct: 496 RDEDAEQLALEALRIREIAFGKDCLPV----------------------------GEALD 555

Query: 541 CLVCIQSRVGKDEKELLNMLMRILRIQEKEFGMEGKEVIDTLKKIVFYMDKLGIKDEKFP 600
           CLVCIQSRVGKDEKELLNMLMRILRIQEKEFGMEGKEVIDTLKKIVFYMDKLGIKDEKFP
Sbjct: 556 CLVCIQSRVGKDEKELLNMLMRILRIQEKEFGMEGKEVIDTLKKIVFYMDKLGIKDEKFP 567

Query: 601 LQKRLSMLRMKFKNQMQY 618
           LQKRLSMLRMKFKNQMQY
Sbjct: 616 LQKRLSMLRMKFKNQMQY 567

BLAST of MS006193 vs. NCBI nr
Match: XP_023522378.1 (nephrocystin-3-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 885.6 bits (2287), Expect = 2.5e-253
Identity = 478/616 (77.60%), Postives = 514/616 (83.44%), Query Frame = 0

Query: 2   MSEICMCMQSLQLQKGIKFTCTSVCLQRQKCNVKLYAVPVRAFSCHSSSTPSASSRADLR 61
           MSEIC+CMQSLQLQKG   TC+SV LQ+QKCN+KLYAVPVRAFSC  +ST SASSRADL 
Sbjct: 1   MSEICLCMQSLQLQKGT--TCSSVSLQKQKCNIKLYAVPVRAFSCRFASTGSASSRADL- 60

Query: 62  WGRRKHVPSTFTAPNGYQSTGHVHYRTDGNNISELESQLEELFNEVRMMIMSGRKNDAVE 121
             +RKH+ S FT PNGYQ  GH+HYRTDGN+ SE E QL+ELFNEVRM+I+SGRKNDAVE
Sbjct: 61  GSQRKHIASAFTVPNGYQRAGHMHYRTDGNSTSEFEGQLDELFNEVRMLIVSGRKNDAVE 120

Query: 122 LLEANYEAVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDNLKDGE 181
           LL+ANYEAVKEQMESGA+GIEQAAVLDIVALGYITVGDLKFVASILDILN +VD+LKD E
Sbjct: 121 LLQANYEAVKEQMESGASGIEQAAVLDIVALGYITVGDLKFVASILDILNSIVDSLKDSE 180

Query: 182 PFLDSVLLHMGSMYSTLKKFEKSMSVYKRAIDIIEKKSGKDSSLLITPILGMAKVIGTIG 241
           PFLDSVLLHMGSMYSTLKK EKS+S YKRAIDIIEKKSG+DSS LITPILGMAKV+GT G
Sbjct: 181 PFLDSVLLHMGSMYSTLKKLEKSVSAYKRAIDIIEKKSGQDSSFLITPILGMAKVLGTNG 240

Query: 242 RTSKAVQFYHRAISILESSRGFENEDLVIPLFNLGDLLLKEGKGKDAEACFARFSLSLSL 301
           RT+KAV+ YHRAISILES+RGFE+EDLVIPLF+LG+LLLKEGKGKDAE CFA        
Sbjct: 241 RTTKAVECYHRAISILESTRGFEDEDLVIPLFSLGNLLLKEGKGKDAETCFA-------- 300

Query: 302 SPSLLPLDYSLNLYALILRIVNIYKNLYGEKDGKVGMAMYSLANAKCARGEAEEAVILYR 361
                             RIVNIYK LYGEKDGKVGMAMYSLANAKCARGEA+EA+ LYR
Sbjct: 301 ------------------RIVNIYKKLYGEKDGKVGMAMYSLANAKCARGEADEAITLYR 360

Query: 362 RALQIIKDSNYMALDDSVMEKMRIDLAELLHVVGRGNEGRELLEECLLINEKSKGKDDPS 421
           RALQII DSNYMALDDS MEKMRIDLAELLH VGR  EGRELLEE LLINEKSKGKD PS
Sbjct: 361 RALQIINDSNYMALDDSEMEKMRIDLAELLHAVGRRKEGRELLEESLLINEKSKGKDHPS 420

Query: 422 SVKHLVNLASSYSRSKNYAEAERLLRMGLNIMVKAAGADDESITVPMLHLAVTLYNLNRD 481
           SVKHLVNLA+SYSRSKNYAEAERLLR+GLNIMVKA G DD+SIT PML+LAVTLYNL RD
Sbjct: 421 SVKHLVNLAASYSRSKNYAEAERLLRIGLNIMVKAVGPDDQSITNPMLNLAVTLYNLKRD 480

Query: 482 EDAEQLALEALRIREIAFGKDCLPVGEKTENCLFLYLVGQSFSGDLICDVLDAGEALDCL 541
           +DAEQLALE LRIRE AFGKDCLPV                            GEALDCL
Sbjct: 481 DDAEQLALEVLRIRENAFGKDCLPV----------------------------GEALDCL 540

Query: 542 VCIQSRVGKDEKELLNMLMRILRIQEKEFGMEGKEVIDTLKKIVFYMDKLGIKDEKFPLQ 601
           V IQSR+GKDE ELL +L RILRIQEK FG E KEVIDTLKKIVFYMDKLG+KDEKFP+Q
Sbjct: 541 VSIQSRLGKDETELLKLLKRILRIQEKAFGYEAKEVIDTLKKIVFYMDKLGLKDEKFPVQ 559

Query: 602 KRLSMLRMKFKNQMQY 618
           KRLSMLRMKFKNQMQY
Sbjct: 601 KRLSMLRMKFKNQMQY 559

BLAST of MS006193 vs. NCBI nr
Match: XP_022995907.1 (nephrocystin-3 isoform X3 [Cucurbita maxima] >XP_022995908.1 nephrocystin-3 isoform X3 [Cucurbita maxima])

HSP 1 Score: 885.2 bits (2286), Expect = 3.3e-253
Identity = 479/618 (77.51%), Postives = 517/618 (83.66%), Query Frame = 0

Query: 1   RMSEICMCMQSLQLQKGIKFTCTSVCLQRQKCNVKLYAVPVRAFSCHSSSTPSASSRADL 60
           RMSEIC+CMQSLQLQKGI  TC+SV LQ+QKCN+KLYAVPVRAFSC  +ST SASSR DL
Sbjct: 16  RMSEICLCMQSLQLQKGI--TCSSVSLQKQKCNIKLYAVPVRAFSCRFASTGSASSRDDL 75

Query: 61  RWGRRKHVPSTFTAPNGYQS-TGHVHYRTDGNNISELESQLEELFNEVRMMIMSGRKNDA 120
              +RKH+ S FT PNGYQS  GH+HYRTDGN+ SE E QL+ELFNEVRM+I+S RK+DA
Sbjct: 76  -GSQRKHIASAFTVPNGYQSRAGHMHYRTDGNSASEFEGQLDELFNEVRMLIVSRRKSDA 135

Query: 121 VELLEANYEAVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDNLKD 180
           VELL+ANYEAVKEQMESGA GIEQAAVLDIVALGYITVGDLKFVASILDILN +VD+LKD
Sbjct: 136 VELLQANYEAVKEQMESGACGIEQAAVLDIVALGYITVGDLKFVASILDILNNIVDSLKD 195

Query: 181 GEPFLDSVLLHMGSMYSTLKKFEKSMSVYKRAIDIIEKKSGKDSSLLITPILGMAKVIGT 240
            EPFLDSVLLHMGSMYSTLKK +KS+S YKRAIDIIEKKSGKDSS LITPILGMAKV+GT
Sbjct: 196 NEPFLDSVLLHMGSMYSTLKKLDKSVSAYKRAIDIIEKKSGKDSSFLITPILGMAKVLGT 255

Query: 241 IGRTSKAVQFYHRAISILESSRGFENEDLVIPLFNLGDLLLKEGKGKDAEACFARFSLSL 300
            G+T+KAV+FYHRAISILES RGFE+EDLVIPLF+LG+LLLKEGKGKDAE CFA      
Sbjct: 256 SGKTTKAVEFYHRAISILESIRGFEDEDLVIPLFSLGNLLLKEGKGKDAETCFA------ 315

Query: 301 SLSPSLLPLDYSLNLYALILRIVNIYKNLYGEKDGKVGMAMYSLANAKCARGEAEEAVIL 360
                               RIVNIYK LYGEKDGKVGMAMYSLANAKCARGEA+EA+ L
Sbjct: 316 --------------------RIVNIYKKLYGEKDGKVGMAMYSLANAKCARGEADEAITL 375

Query: 361 YRRALQIIKDSNYMALDDSVMEKMRIDLAELLHVVGRGNEGRELLEECLLINEKSKGKDD 420
           YRRALQIIKDSNYMALDDS MEKMRIDLAELLH VGRG EGRELLEECLLINEKSKGKD 
Sbjct: 376 YRRALQIIKDSNYMALDDSEMEKMRIDLAELLHAVGRGKEGRELLEECLLINEKSKGKDH 435

Query: 421 PSSVKHLVNLASSYSRSKNYAEAERLLRMGLNIMVKAAGADDESITVPMLHLAVTLYNLN 480
           PSSVKHLVNLA+SYSRSKNYAEAERLLR+GL+IMVKA G DD+SIT PML+LAVTLYNL 
Sbjct: 436 PSSVKHLVNLAASYSRSKNYAEAERLLRIGLDIMVKAVGPDDQSITNPMLNLAVTLYNLK 495

Query: 481 RDEDAEQLALEALRIREIAFGKDCLPVGEKTENCLFLYLVGQSFSGDLICDVLDAGEALD 540
           RD+DAEQLALE LRIRE AFGKDCLPV                            GEALD
Sbjct: 496 RDDDAEQLALEVLRIRENAFGKDCLPV----------------------------GEALD 555

Query: 541 CLVCIQSRVGKDEKELLNMLMRILRIQEKEFGMEGKEVIDTLKKIVFYMDKLGIKDEKFP 600
           CLV IQSR+GKDE ELL +L RILRIQEK FG E KEVIDTLKKIVFYMDKLG+KDEKFP
Sbjct: 556 CLVSIQSRLGKDETELLKLLKRILRIQEKAFGYEAKEVIDTLKKIVFYMDKLGLKDEKFP 576

Query: 601 LQKRLSMLRMKFKNQMQY 618
           +QKRLS+LRMKFKNQMQY
Sbjct: 616 VQKRLSLLRMKFKNQMQY 576

BLAST of MS006193 vs. ExPASy Swiss-Prot
Match: Q07866 (Kinesin light chain 1 OS=Homo sapiens OX=9606 GN=KLC1 PE=1 SV=2)

HSP 1 Score: 65.5 bits (158), Expect = 2.5e-09
Identity = 51/179 (28.49%), Postives = 80/179 (44.69%), Query Frame = 0

Query: 340 MYSLANAKCARGEAEEAVILYRRALQIIKDSNYMALDDSVMEKMRIDLAELLHVVGRGNE 399
           +++L     ++G  E AV L ++AL+ ++ ++    D   +  M   LA +     +  +
Sbjct: 216 LHNLVIQYASQGRYEVAVPLCKQALEDLEKTS--GHDHPDVATMLNILALVYRDQNKYKD 275

Query: 400 GRELLEECLLINEKSKGKDDPSSVKHLVNLASSYSRSKNYAEAERLLRMGLNIMVKAAGA 459
              LL + L I EK+ GKD P+    L NLA  Y +   Y EAE L +  L I  K  G 
Sbjct: 276 AANLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGK 335

Query: 460 DDESITVPMLHLAVTLYNLNRDEDAEQLALEALRIREIAFGKDCLPVGEKTENCLFLYL 519
           D   +   + +LA+   N  + E+ E     AL I +   G D   V +   N    YL
Sbjct: 336 DHPDVAKQLNNLALLCQNQGKYEEVEYYYQRALEIYQTKLGPDDPNVAKTKNNLASCYL 392

BLAST of MS006193 vs. ExPASy Swiss-Prot
Match: Q5R581 (Kinesin light chain 1 OS=Pongo abelii OX=9601 GN=KLC1 PE=2 SV=3)

HSP 1 Score: 65.5 bits (158), Expect = 2.5e-09
Identity = 51/179 (28.49%), Postives = 80/179 (44.69%), Query Frame = 0

Query: 340 MYSLANAKCARGEAEEAVILYRRALQIIKDSNYMALDDSVMEKMRIDLAELLHVVGRGNE 399
           +++L     ++G  E AV L ++AL+ ++ ++    D   +  M   LA +     +  +
Sbjct: 216 LHNLVIQYASQGRYEVAVPLCKQALEDLEKTS--GHDHPDVATMLNILALVYRDQNKYKD 275

Query: 400 GRELLEECLLINEKSKGKDDPSSVKHLVNLASSYSRSKNYAEAERLLRMGLNIMVKAAGA 459
              LL + L I EK+ GKD P+    L NLA  Y +   Y EAE L +  L I  K  G 
Sbjct: 276 AANLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGK 335

Query: 460 DDESITVPMLHLAVTLYNLNRDEDAEQLALEALRIREIAFGKDCLPVGEKTENCLFLYL 519
           D   +   + +LA+   N  + E+ E     AL I +   G D   V +   N    YL
Sbjct: 336 DHPDVAKQLNNLALLCQNQGKYEEVEYYYQRALEIYQTKLGPDDPNVAKTKNNLASCYL 392

BLAST of MS006193 vs. ExPASy Swiss-Prot
Match: Q9DBS5 (Kinesin light chain 4 OS=Mus musculus OX=10090 GN=Klc4 PE=1 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 2.5e-09
Identity = 55/199 (27.64%), Postives = 95/199 (47.74%), Query Frame = 0

Query: 95  ELESQLEELFNEVRMMIMSGRKNDAVELLEANYEAVKEQMESGANGIEQAAVLDIVALGY 154
           E+ ++L  L N V      GR   AV L +   E +  +  SG    + A +L+I+AL Y
Sbjct: 206 EIPARLRTLHNLVIQYAAQGRYEVAVPLCKQALEDL--ERTSGRGHPDVATMLNILALVY 265

Query: 155 ITVGDLKFVASIL-DILNKVVDNLKDGEPFLDSVLLHMGSMYSTLKKFEKSMSVYKRAID 214
                 K  A +L D L+     L    P + + L ++  +Y    K++++  + +RA++
Sbjct: 266 RDQNKYKEAAHLLNDALSIRESTLGRDHPAVAATLNNLAVLYGKRGKYKEAEPLCQRALE 325

Query: 215 IIEKKSGKDSSLLITPILGMAKVIGTIGRTSKAVQFYHRAISILESSRGFENEDLVIPLF 274
           I EK  G D   +   +  +A +    G+     ++Y RA++I ES  G +N ++     
Sbjct: 326 IREKVLGTDHPDVAKQLNNLALLCQNQGKYEAVERYYQRALAIYESQLGPDNPNVARTKN 385

Query: 275 NLGDLLLKEGKGKDAEACF 293
           NL    LK+GK  +AEA +
Sbjct: 386 NLASCYLKQGKYSEAEALY 402

BLAST of MS006193 vs. ExPASy Swiss-Prot
Match: P46822 (Kinesin light chain OS=Caenorhabditis elegans OX=6239 GN=klc-2 PE=1 SV=2)

HSP 1 Score: 64.7 bits (156), Expect = 4.2e-09
Identity = 53/199 (26.63%), Postives = 98/199 (49.25%), Query Frame = 0

Query: 95  ELESQLEELFNEVRMMIMSGRKNDAVELLEANYEAVKEQMESGANGIEQAAVLDIVALGY 154
           E+ ++L  L N V      GR   AV L +   E +++   SG +  + A +L+I+AL Y
Sbjct: 201 EIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEK--TSGHDHPDVATMLNILALVY 260

Query: 155 ITVGDLKFVASILDILNKVVDN-LKDGEPFLDSVLLHMGSMYSTLKKFEKSMSVYKRAID 214
                 K  A++L+    + +  L +  P + + L ++  ++    KF+ +  + KRA++
Sbjct: 261 RDQNKYKEAANLLNEALSIREKCLGESHPAVAATLNNLAVLFGKRGKFKDAEPLCKRALE 320

Query: 215 IIEKKSGKDSSLLITPILGMAKVIGTIGRTSKAVQFYHRAISILESSRGFENEDLVIPLF 274
           I EK  G D   +   +  +A +    G+  +  ++Y RA+ I ES  G ++ ++     
Sbjct: 321 IREKVLGDDHPDVAKQLNNLALLCQNQGKYEEVEKYYKRALEIYESKLGPDDPNVAKTKN 380

Query: 275 NLGDLLLKEGKGKDAEACF 293
           NL    LK+GK K+AE  +
Sbjct: 381 NLSSAYLKQGKYKEAEELY 397

BLAST of MS006193 vs. ExPASy Swiss-Prot
Match: P37285 (Kinesin light chain 1 OS=Rattus norvegicus OX=10116 GN=Klc1 PE=1 SV=2)

HSP 1 Score: 64.3 bits (155), Expect = 5.5e-09
Identity = 50/179 (27.93%), Postives = 80/179 (44.69%), Query Frame = 0

Query: 340 MYSLANAKCARGEAEEAVILYRRALQIIKDSNYMALDDSVMEKMRIDLAELLHVVGRGNE 399
           +++L     ++G  E AV L ++AL+ ++ ++    D   +  M   LA +     +  +
Sbjct: 216 LHNLVIQYASQGRYEVAVPLCKQALEDLEKTS--GHDHPDVATMLNILALVYRDQNKYKD 275

Query: 400 GRELLEECLLINEKSKGKDDPSSVKHLVNLASSYSRSKNYAEAERLLRMGLNIMVKAAGA 459
              LL + L I EK+ G+D P+    L NLA  Y +   Y EAE L +  L I  K  G 
Sbjct: 276 AANLLNDALAIREKTLGRDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGK 335

Query: 460 DDESITVPMLHLAVTLYNLNRDEDAEQLALEALRIREIAFGKDCLPVGEKTENCLFLYL 519
           D   +   + +LA+   N  + E+ E     AL I +   G D   V +   N    YL
Sbjct: 336 DHPDVAKQLNNLALLCQNQGKYEEVEYYYQRALEIYQTKLGPDDPNVAKTKNNLASCYL 392

BLAST of MS006193 vs. ExPASy TrEMBL
Match: A0A6J1DYD7 (uncharacterized protein LOC111025715 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111025733 PE=4 SV=1)

HSP 1 Score: 1053.1 bits (2722), Expect = 4.4e-304
Identity = 562/617 (91.09%), Postives = 562/617 (91.09%), Query Frame = 0

Query: 1   RMSEICMCMQSLQLQKGIKFTCTSVCLQRQKCNVKLYAVPVRAFSCHSSSTPSASSRADL 60
           RMSEICMCMQSLQLQKGIKFTCTSVCLQRQKCNVKLYAVPVRAFSCHSSSTPSASSRADL
Sbjct: 16  RMSEICMCMQSLQLQKGIKFTCTSVCLQRQKCNVKLYAVPVRAFSCHSSSTPSASSRADL 75

Query: 61  RWGRRKHVPSTFTAPNGYQSTGHVHYRTDGNNISELESQLEELFNEVRMMIMSGRKNDAV 120
           RWGRRKHVPSTFTAPNGYQSTGHVHYRTD NNISELESQLEELFNEVRMMIMSGRKNDAV
Sbjct: 76  RWGRRKHVPSTFTAPNGYQSTGHVHYRTDENNISELESQLEELFNEVRMMIMSGRKNDAV 135

Query: 121 ELLEANYEAVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDNLKDG 180
           ELLEANYEAVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDNLKDG
Sbjct: 136 ELLEANYEAVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDNLKDG 195

Query: 181 EPFLDSVLLHMGSMYSTLKKFEKSMSVYKRAIDIIEKKSGKDSSLLITPILGMAKVIGTI 240
           EPFLDSVLLHMGSMYSTLKKFEKSMSVYKRAIDIIEKKSGKDSSLLITPILGMAKVIGTI
Sbjct: 196 EPFLDSVLLHMGSMYSTLKKFEKSMSVYKRAIDIIEKKSGKDSSLLITPILGMAKVIGTI 255

Query: 241 GRTSKAVQFYHRAISILESSRGFENEDLVIPLFNLGDLLLKEGKGKDAEACFARFSLSLS 300
           GRTSKAVQFYHRAISILESSRGFENEDLVIPLFNLGDLLLKEGKGKDAEACFA       
Sbjct: 256 GRTSKAVQFYHRAISILESSRGFENEDLVIPLFNLGDLLLKEGKGKDAEACFA------- 315

Query: 301 LSPSLLPLDYSLNLYALILRIVNIYKNLYGEKDGKVGMAMYSLANAKCARGEAEEAVILY 360
                              RIVNIYKNLYGEKDGKVGMAMYSLANAKCARGEAEEAVILY
Sbjct: 316 -------------------RIVNIYKNLYGEKDGKVGMAMYSLANAKCARGEAEEAVILY 375

Query: 361 RRALQIIKDSNYMALDDSVMEKMRIDLAELLHVVGRGNEGRELLEECLLINEKSKGKDDP 420
           RRALQIIKDSNYMALDDSVMEKMRIDLAELLHVVGRGNEGRELLEECLLINEKSKGKDDP
Sbjct: 376 RRALQIIKDSNYMALDDSVMEKMRIDLAELLHVVGRGNEGRELLEECLLINEKSKGKDDP 435

Query: 421 SSVKHLVNLASSYSRSKNYAEAERLLRMGLNIMVKAAGADDESITVPMLHLAVTLYNLNR 480
           SSVKHLVNLASSYSRSKNYAEAERLLRMGLNIMVKAAGADDESITVPMLHLAVTLYNLNR
Sbjct: 436 SSVKHLVNLASSYSRSKNYAEAERLLRMGLNIMVKAAGADDESITVPMLHLAVTLYNLNR 495

Query: 481 DEDAEQLALEALRIREIAFGKDCLPVGEKTENCLFLYLVGQSFSGDLICDVLDAGEALDC 540
           DEDAEQLALEALRIREIAFGKDCLPV                            GEALDC
Sbjct: 496 DEDAEQLALEALRIREIAFGKDCLPV----------------------------GEALDC 555

Query: 541 LVCIQSRVGKDEKELLNMLMRILRIQEKEFGMEGKEVIDTLKKIVFYMDKLGIKDEKFPL 600
           LVCIQSRVGKDEKELLNMLMRILRIQEKEFGMEGKEVIDTLKKIVFYMDKLGIKDEKFPL
Sbjct: 556 LVCIQSRVGKDEKELLNMLMRILRIQEKEFGMEGKEVIDTLKKIVFYMDKLGIKDEKFPL 578

Query: 601 QKRLSMLRMKFKNQMQY 618
           QKRLSMLRMKFKNQMQY
Sbjct: 616 QKRLSMLRMKFKNQMQY 578

BLAST of MS006193 vs. ExPASy TrEMBL
Match: A0A6J1E240 (uncharacterized protein LOC111025715 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111025733 PE=4 SV=1)

HSP 1 Score: 1048.5 bits (2710), Expect = 1.1e-302
Identity = 562/618 (90.94%), Postives = 562/618 (90.94%), Query Frame = 0

Query: 1   RMSEICMCMQSLQLQKGIKFTCTSVCLQRQKCNVKLYAVPVRAFSCHSSSTPSASSRADL 60
           RMSEICMCMQSLQLQKGIKFTCTSVCLQRQKCNVKLYAVPVRAFSCHSSSTPSASSRADL
Sbjct: 16  RMSEICMCMQSLQLQKGIKFTCTSVCLQRQKCNVKLYAVPVRAFSCHSSSTPSASSRADL 75

Query: 61  RWGRRKHVPSTFTAPNGYQ-STGHVHYRTDGNNISELESQLEELFNEVRMMIMSGRKNDA 120
           RWGRRKHVPSTFTAPNGYQ STGHVHYRTD NNISELESQLEELFNEVRMMIMSGRKNDA
Sbjct: 76  RWGRRKHVPSTFTAPNGYQSSTGHVHYRTDENNISELESQLEELFNEVRMMIMSGRKNDA 135

Query: 121 VELLEANYEAVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDNLKD 180
           VELLEANYEAVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDNLKD
Sbjct: 136 VELLEANYEAVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDNLKD 195

Query: 181 GEPFLDSVLLHMGSMYSTLKKFEKSMSVYKRAIDIIEKKSGKDSSLLITPILGMAKVIGT 240
           GEPFLDSVLLHMGSMYSTLKKFEKSMSVYKRAIDIIEKKSGKDSSLLITPILGMAKVIGT
Sbjct: 196 GEPFLDSVLLHMGSMYSTLKKFEKSMSVYKRAIDIIEKKSGKDSSLLITPILGMAKVIGT 255

Query: 241 IGRTSKAVQFYHRAISILESSRGFENEDLVIPLFNLGDLLLKEGKGKDAEACFARFSLSL 300
           IGRTSKAVQFYHRAISILESSRGFENEDLVIPLFNLGDLLLKEGKGKDAEACFA      
Sbjct: 256 IGRTSKAVQFYHRAISILESSRGFENEDLVIPLFNLGDLLLKEGKGKDAEACFA------ 315

Query: 301 SLSPSLLPLDYSLNLYALILRIVNIYKNLYGEKDGKVGMAMYSLANAKCARGEAEEAVIL 360
                               RIVNIYKNLYGEKDGKVGMAMYSLANAKCARGEAEEAVIL
Sbjct: 316 --------------------RIVNIYKNLYGEKDGKVGMAMYSLANAKCARGEAEEAVIL 375

Query: 361 YRRALQIIKDSNYMALDDSVMEKMRIDLAELLHVVGRGNEGRELLEECLLINEKSKGKDD 420
           YRRALQIIKDSNYMALDDSVMEKMRIDLAELLHVVGRGNEGRELLEECLLINEKSKGKDD
Sbjct: 376 YRRALQIIKDSNYMALDDSVMEKMRIDLAELLHVVGRGNEGRELLEECLLINEKSKGKDD 435

Query: 421 PSSVKHLVNLASSYSRSKNYAEAERLLRMGLNIMVKAAGADDESITVPMLHLAVTLYNLN 480
           PSSVKHLVNLASSYSRSKNYAEAERLLRMGLNIMVKAAGADDESITVPMLHLAVTLYNLN
Sbjct: 436 PSSVKHLVNLASSYSRSKNYAEAERLLRMGLNIMVKAAGADDESITVPMLHLAVTLYNLN 495

Query: 481 RDEDAEQLALEALRIREIAFGKDCLPVGEKTENCLFLYLVGQSFSGDLICDVLDAGEALD 540
           RDEDAEQLALEALRIREIAFGKDCLPV                            GEALD
Sbjct: 496 RDEDAEQLALEALRIREIAFGKDCLPV----------------------------GEALD 555

Query: 541 CLVCIQSRVGKDEKELLNMLMRILRIQEKEFGMEGKEVIDTLKKIVFYMDKLGIKDEKFP 600
           CLVCIQSRVGKDEKELLNMLMRILRIQEKEFGMEGKEVIDTLKKIVFYMDKLGIKDEKFP
Sbjct: 556 CLVCIQSRVGKDEKELLNMLMRILRIQEKEFGMEGKEVIDTLKKIVFYMDKLGIKDEKFP 579

Query: 601 LQKRLSMLRMKFKNQMQY 618
           LQKRLSMLRMKFKNQMQY
Sbjct: 616 LQKRLSMLRMKFKNQMQY 579

BLAST of MS006193 vs. ExPASy TrEMBL
Match: A0A6J1E3I7 (uncharacterized protein LOC111025715 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111025715 PE=4 SV=1)

HSP 1 Score: 1018.1 bits (2631), Expect = 1.6e-293
Identity = 550/618 (89.00%), Postives = 550/618 (89.00%), Query Frame = 0

Query: 1   RMSEICMCMQSLQLQKGIKFTCTSVCLQRQKCNVKLYAVPVRAFSCHSSSTPSASSRADL 60
           RMSEICMCMQSLQLQKGIKFTCTSVCLQRQKCNVKLYAVPVRAFSCHSSSTPSASSRADL
Sbjct: 16  RMSEICMCMQSLQLQKGIKFTCTSVCLQRQKCNVKLYAVPVRAFSCHSSSTPSASSRADL 75

Query: 61  RWGRRKHVPSTFTAPNGYQ-STGHVHYRTDGNNISELESQLEELFNEVRMMIMSGRKNDA 120
           RWGRRKHVPSTFTAPNGYQ STGHVHYRTD NNISELESQLEELFNEVRMMIMSGRKNDA
Sbjct: 76  RWGRRKHVPSTFTAPNGYQSSTGHVHYRTDENNISELESQLEELFNEVRMMIMSGRKNDA 135

Query: 121 VELLEANYEAVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDNLKD 180
           VELLEANYEAVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDNLKD
Sbjct: 136 VELLEANYEAVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDNLKD 195

Query: 181 GEPFLDSVLLHMGSMYSTLKKFEKSMSVYKRAIDIIEKKSGKDSSLLITPILGMAKVIGT 240
           GEPFLDSVLLHMGSMYSTLKKFEKSMSVYKRAIDIIEKKS            GMAKVIGT
Sbjct: 196 GEPFLDSVLLHMGSMYSTLKKFEKSMSVYKRAIDIIEKKS------------GMAKVIGT 255

Query: 241 IGRTSKAVQFYHRAISILESSRGFENEDLVIPLFNLGDLLLKEGKGKDAEACFARFSLSL 300
           IGRTSKAVQFYHRAISILESSRGFENEDLVIPLFNLGDLLLKEGKGKDAEACFA      
Sbjct: 256 IGRTSKAVQFYHRAISILESSRGFENEDLVIPLFNLGDLLLKEGKGKDAEACFA------ 315

Query: 301 SLSPSLLPLDYSLNLYALILRIVNIYKNLYGEKDGKVGMAMYSLANAKCARGEAEEAVIL 360
                               RIVNIYKNLYGEKDGKVGMAMYSLANAKCARGEAEEAVIL
Sbjct: 316 --------------------RIVNIYKNLYGEKDGKVGMAMYSLANAKCARGEAEEAVIL 375

Query: 361 YRRALQIIKDSNYMALDDSVMEKMRIDLAELLHVVGRGNEGRELLEECLLINEKSKGKDD 420
           YRRALQIIKDSNYMALDDSVMEKMRIDLAELLHVVGRGNEGRELLEECLLINEKSKGKDD
Sbjct: 376 YRRALQIIKDSNYMALDDSVMEKMRIDLAELLHVVGRGNEGRELLEECLLINEKSKGKDD 435

Query: 421 PSSVKHLVNLASSYSRSKNYAEAERLLRMGLNIMVKAAGADDESITVPMLHLAVTLYNLN 480
           PSSVKHLVNLASSYSRSKNYAEAERLLRMGLNIMVKAAGADDESITVPMLHLAVTLYNLN
Sbjct: 436 PSSVKHLVNLASSYSRSKNYAEAERLLRMGLNIMVKAAGADDESITVPMLHLAVTLYNLN 495

Query: 481 RDEDAEQLALEALRIREIAFGKDCLPVGEKTENCLFLYLVGQSFSGDLICDVLDAGEALD 540
           RDEDAEQLALEALRIREIAFGKDCLPV                            GEALD
Sbjct: 496 RDEDAEQLALEALRIREIAFGKDCLPV----------------------------GEALD 555

Query: 541 CLVCIQSRVGKDEKELLNMLMRILRIQEKEFGMEGKEVIDTLKKIVFYMDKLGIKDEKFP 600
           CLVCIQSRVGKDEKELLNMLMRILRIQEKEFGMEGKEVIDTLKKIVFYMDKLGIKDEKFP
Sbjct: 556 CLVCIQSRVGKDEKELLNMLMRILRIQEKEFGMEGKEVIDTLKKIVFYMDKLGIKDEKFP 567

Query: 601 LQKRLSMLRMKFKNQMQY 618
           LQKRLSMLRMKFKNQMQY
Sbjct: 616 LQKRLSMLRMKFKNQMQY 567

BLAST of MS006193 vs. ExPASy TrEMBL
Match: A0A6J1K9C6 (nephrocystin-3 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111491290 PE=4 SV=1)

HSP 1 Score: 885.2 bits (2286), Expect = 1.6e-253
Identity = 479/618 (77.51%), Postives = 517/618 (83.66%), Query Frame = 0

Query: 1   RMSEICMCMQSLQLQKGIKFTCTSVCLQRQKCNVKLYAVPVRAFSCHSSSTPSASSRADL 60
           RMSEIC+CMQSLQLQKGI  TC+SV LQ+QKCN+KLYAVPVRAFSC  +ST SASSR DL
Sbjct: 16  RMSEICLCMQSLQLQKGI--TCSSVSLQKQKCNIKLYAVPVRAFSCRFASTGSASSRDDL 75

Query: 61  RWGRRKHVPSTFTAPNGYQS-TGHVHYRTDGNNISELESQLEELFNEVRMMIMSGRKNDA 120
              +RKH+ S FT PNGYQS  GH+HYRTDGN+ SE E QL+ELFNEVRM+I+S RK+DA
Sbjct: 76  -GSQRKHIASAFTVPNGYQSRAGHMHYRTDGNSASEFEGQLDELFNEVRMLIVSRRKSDA 135

Query: 121 VELLEANYEAVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDNLKD 180
           VELL+ANYEAVKEQMESGA GIEQAAVLDIVALGYITVGDLKFVASILDILN +VD+LKD
Sbjct: 136 VELLQANYEAVKEQMESGACGIEQAAVLDIVALGYITVGDLKFVASILDILNNIVDSLKD 195

Query: 181 GEPFLDSVLLHMGSMYSTLKKFEKSMSVYKRAIDIIEKKSGKDSSLLITPILGMAKVIGT 240
            EPFLDSVLLHMGSMYSTLKK +KS+S YKRAIDIIEKKSGKDSS LITPILGMAKV+GT
Sbjct: 196 NEPFLDSVLLHMGSMYSTLKKLDKSVSAYKRAIDIIEKKSGKDSSFLITPILGMAKVLGT 255

Query: 241 IGRTSKAVQFYHRAISILESSRGFENEDLVIPLFNLGDLLLKEGKGKDAEACFARFSLSL 300
            G+T+KAV+FYHRAISILES RGFE+EDLVIPLF+LG+LLLKEGKGKDAE CFA      
Sbjct: 256 SGKTTKAVEFYHRAISILESIRGFEDEDLVIPLFSLGNLLLKEGKGKDAETCFA------ 315

Query: 301 SLSPSLLPLDYSLNLYALILRIVNIYKNLYGEKDGKVGMAMYSLANAKCARGEAEEAVIL 360
                               RIVNIYK LYGEKDGKVGMAMYSLANAKCARGEA+EA+ L
Sbjct: 316 --------------------RIVNIYKKLYGEKDGKVGMAMYSLANAKCARGEADEAITL 375

Query: 361 YRRALQIIKDSNYMALDDSVMEKMRIDLAELLHVVGRGNEGRELLEECLLINEKSKGKDD 420
           YRRALQIIKDSNYMALDDS MEKMRIDLAELLH VGRG EGRELLEECLLINEKSKGKD 
Sbjct: 376 YRRALQIIKDSNYMALDDSEMEKMRIDLAELLHAVGRGKEGRELLEECLLINEKSKGKDH 435

Query: 421 PSSVKHLVNLASSYSRSKNYAEAERLLRMGLNIMVKAAGADDESITVPMLHLAVTLYNLN 480
           PSSVKHLVNLA+SYSRSKNYAEAERLLR+GL+IMVKA G DD+SIT PML+LAVTLYNL 
Sbjct: 436 PSSVKHLVNLAASYSRSKNYAEAERLLRIGLDIMVKAVGPDDQSITNPMLNLAVTLYNLK 495

Query: 481 RDEDAEQLALEALRIREIAFGKDCLPVGEKTENCLFLYLVGQSFSGDLICDVLDAGEALD 540
           RD+DAEQLALE LRIRE AFGKDCLPV                            GEALD
Sbjct: 496 RDDDAEQLALEVLRIRENAFGKDCLPV----------------------------GEALD 555

Query: 541 CLVCIQSRVGKDEKELLNMLMRILRIQEKEFGMEGKEVIDTLKKIVFYMDKLGIKDEKFP 600
           CLV IQSR+GKDE ELL +L RILRIQEK FG E KEVIDTLKKIVFYMDKLG+KDEKFP
Sbjct: 556 CLVSIQSRLGKDETELLKLLKRILRIQEKAFGYEAKEVIDTLKKIVFYMDKLGLKDEKFP 576

Query: 601 LQKRLSMLRMKFKNQMQY 618
           +QKRLS+LRMKFKNQMQY
Sbjct: 616 VQKRLSLLRMKFKNQMQY 576

BLAST of MS006193 vs. ExPASy TrEMBL
Match: A0A6J1H2E0 (nephrocystin-3 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111459474 PE=4 SV=1)

HSP 1 Score: 884.8 bits (2285), Expect = 2.1e-253
Identity = 479/618 (77.51%), Postives = 517/618 (83.66%), Query Frame = 0

Query: 1   RMSEICMCMQSLQLQKGIKFTCTSVCLQRQKCNVKLYAVPVRAFSCHSSSTPSASSRADL 60
           RMSEIC+CMQSLQLQKGI  TC+SV LQ+QKCN+KLYAVPVRAFSC  +ST SASSRADL
Sbjct: 16  RMSEICLCMQSLQLQKGI--TCSSVSLQKQKCNIKLYAVPVRAFSCQFASTGSASSRADL 75

Query: 61  RWGRRKHVPSTFTAPNGYQS-TGHVHYRTDGNNISELESQLEELFNEVRMMIMSGRKNDA 120
              +RKH+ S FT PNGYQS  GH+HYRTDG + SE E QL+ELFNEVRM+I+SGRK+DA
Sbjct: 76  -GSQRKHIASAFTVPNGYQSRPGHMHYRTDGTSTSEFEGQLDELFNEVRMLIVSGRKSDA 135

Query: 121 VELLEANYEAVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDNLKD 180
           VELL+ANYEAVKEQMESGA GIEQAAVLDIVALGYITVGDLKFVASILDILN +VD+LKD
Sbjct: 136 VELLQANYEAVKEQMESGAIGIEQAAVLDIVALGYITVGDLKFVASILDILNNIVDSLKD 195

Query: 181 GEPFLDSVLLHMGSMYSTLKKFEKSMSVYKRAIDIIEKKSGKDSSLLITPILGMAKVIGT 240
            EPFLDSVLLHMGSMYSTLKK EKSMS YKRAIDIIEKKSGKDSS LITPILGMAKV+GT
Sbjct: 196 NEPFLDSVLLHMGSMYSTLKKLEKSMSAYKRAIDIIEKKSGKDSSFLITPILGMAKVLGT 255

Query: 241 IGRTSKAVQFYHRAISILESSRGFENEDLVIPLFNLGDLLLKEGKGKDAEACFARFSLSL 300
            G+T+KAV+ YHRAISILES+RGFE+EDLVIPLF+LG+LLLKEGKGKDAE CFA      
Sbjct: 256 SGKTTKAVESYHRAISILESTRGFEDEDLVIPLFSLGNLLLKEGKGKDAETCFA------ 315

Query: 301 SLSPSLLPLDYSLNLYALILRIVNIYKNLYGEKDGKVGMAMYSLANAKCARGEAEEAVIL 360
                               RIVNIYK LYGEKDGKVGMAMYSLANAKCARGEA+EA+ L
Sbjct: 316 --------------------RIVNIYKKLYGEKDGKVGMAMYSLANAKCARGEADEAITL 375

Query: 361 YRRALQIIKDSNYMALDDSVMEKMRIDLAELLHVVGRGNEGRELLEECLLINEKSKGKDD 420
           YRRALQIIKDSNYMALDDS MEKMRIDLAELLH VGRG EGRELLEECLLINEKSKGKD 
Sbjct: 376 YRRALQIIKDSNYMALDDSEMEKMRIDLAELLHAVGRGKEGRELLEECLLINEKSKGKDH 435

Query: 421 PSSVKHLVNLASSYSRSKNYAEAERLLRMGLNIMVKAAGADDESITVPMLHLAVTLYNLN 480
           PSSVKHLVNLA+SYSRSKNYAEAERLLR+GL+IMVKA G DD+SIT PML+LAVTLYNL 
Sbjct: 436 PSSVKHLVNLAASYSRSKNYAEAERLLRIGLDIMVKAVGPDDQSITNPMLNLAVTLYNLK 495

Query: 481 RDEDAEQLALEALRIREIAFGKDCLPVGEKTENCLFLYLVGQSFSGDLICDVLDAGEALD 540
           RD+DAEQLALE LRIRE AFGKDCLPV                            GEALD
Sbjct: 496 RDDDAEQLALEVLRIRENAFGKDCLPV----------------------------GEALD 555

Query: 541 CLVCIQSRVGKDEKELLNMLMRILRIQEKEFGMEGKEVIDTLKKIVFYMDKLGIKDEKFP 600
           CLV IQSR+GKD+ ELL +L RILRIQEK FG E KEVIDTLKKIVFYMDKLG+KDEKFP
Sbjct: 556 CLVSIQSRLGKDDTELLKLLKRILRIQEKAFGYEAKEVIDTLKKIVFYMDKLGMKDEKFP 576

Query: 601 LQKRLSMLRMKFKNQMQY 618
           +QKRLS+LR KFKNQMQY
Sbjct: 616 VQKRLSLLRTKFKNQMQY 576

BLAST of MS006193 vs. TAIR 10
Match: AT5G53080.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 576.2 bits (1484), Expect = 3.0e-164
Identity = 317/602 (52.66%), Postives = 419/602 (69.60%), Query Frame = 0

Query: 21  TCTSVCLQRQKCNVKLYAVPVRAFSCHSSSTPSASSRADLRWGRRKHVPSTFTAPNGYQS 80
           T   VCL+ QK   KLY +P R    H  STP  S            V S+    + Y +
Sbjct: 33  TWQCVCLRNQKRKPKLYLIPAR----HFLSTPIDS------------VSSSSITASRYAT 92

Query: 81  TG--HVHYRTDGNNISELES---QLEELFNEVRMMIMSGRKNDAVELLEANYEAVKEQME 140
           +G   V   T  NN++E+E    +L+ELFNEV+ M+  G+++DA++LL ANY AVKE+++
Sbjct: 93  SGVSEVQRSTSSNNVTEMEEFEMELQELFNEVKSMVKIGKESDAMDLLRANYVAVKEELD 152

Query: 141 SGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDNLKDGEPFLDSVLLHMGSMY 200
           SG  GIEQAAVLDI+ALGY+ VGDLK V ++LD++NK+VDNLKD EP LDSVL+H+GSMY
Sbjct: 153 SGLKGIEQAAVLDIIALGYMAVGDLKPVPALLDMINKIVDNLKDSEPLLDSVLMHVGSMY 212

Query: 201 STLKKFEKSMSVYKRAIDIIEKKSGKDSSLLITPILGMAKVIGTIGRTSKAVQFYHRAIS 260
           S + KFE ++ V++RAI I+E + GK ++LL+TP+LGMAK   + G+ +KA+  Y R ++
Sbjct: 213 SVIGKFENAILVHQRAIRILENRYGKCNTLLVTPLLGMAKSFASDGKATKAIGVYERTLT 272

Query: 261 ILESSRGFENEDLVIPLFNLGDLLLKEGKGKDAEACFARFSLSLSLSPSLLPLDYSLNLY 320
           ILE +RG E+EDLV+PLF+LG LLLKEGK  +AE  F                       
Sbjct: 273 ILERNRGSESEDLVVPLFSLGKLLLKEGKAAEAEIPFT---------------------- 332

Query: 321 ALILRIVNIYKNLYGEKDGKVGMAMYSLANAKCARGEAEEAVILYRRALQIIKDSNYMAL 380
                IVNIYK +YGE+DG+VGMAM SLANAKC++G+A EAV +YR AL+IIKDSNYM +
Sbjct: 333 ----SIVNIYKKIYGERDGRVGMAMCSLANAKCSKGDANEAVDIYRNALRIIKDSNYMTI 392

Query: 381 DDSVMEKMRIDLAELLHVVGRGNEGRELLEECLLINEKSKGKDDPSSVKHLVNLASSYSR 440
           D+S++E MRIDLAELLH VGRG+EGRELLEECLLINE+ KGK+ PS   HL+NLA+SYSR
Sbjct: 393 DNSILENMRIDLAELLHFVGRGDEGRELLEECLLINERFKGKNHPSMATHLINLAASYSR 452

Query: 441 SKNYAEAERLLRMGLNIMVKAAGADDESITVPMLHLAVTLYNLNRDEDAEQLALEALRIR 500
           SKNY EAERLLR  LNIM  + G++ +SIT PML+LAVTL  LNRDE+AEQ+AL+ LRIR
Sbjct: 453 SKNYVEAERLLRTCLNIMEVSVGSEGQSITFPMLNLAVTLSQLNRDEEAEQIALKVLRIR 512

Query: 501 EIAFGKDCLPVGEKTENCLFLYLVGQSFSGDLICDVLDAGEALDCLVCIQSRVGKDEKEL 560
           E AFG+D LPV                            GEALDCLV IQ+R+G+D+ E+
Sbjct: 513 EKAFGEDSLPV----------------------------GEALDCLVSIQARLGRDDGEI 564

Query: 561 LNMLMRILRIQEKEFGMEGKEVIDTLKKIVFYMDKLGIKDEKFPLQKRLSMLRMKFKNQM 618
           L +L R++ IQEKEFG   +E+I TL+KI+ +++KL +KD+KF  ++RL++LR ++K  +
Sbjct: 573 LGLLKRVMMIQEKEFGPSAQELIVTLQKIIHFLEKLEMKDDKFKFRRRLALLRERYKQSL 564