MS006185 (gene) Bitter gourd (TR) v1

BLAST of MS006185 vs. NCBI nr
Match: XP_038906871.1 (glucan endo-1,3-beta-glucosidase [Benincasa hispida])

HSP 1 Score: 760.0 bits (1961), Expect = 1.2e-215
Identity = 373/463 (80.56%), Postives = 409/463 (88.34%), Query Frame = 0

Query: 4   IYVHTSIIVGGFLHYL-----CSGAKGGSIGVNYGTVADNLPPPSKVAQFLSDSTIINRV 63
           ++VH S  V  FL  L       GAK GSIGVNYGT+ADNLPPPSKVA FL DSTII+RV
Sbjct: 40  LFVHIS-TVSTFLLLLPTLIQIQGAKDGSIGVNYGTLADNLPPPSKVAAFLLDSTIIDRV 99

Query: 64  RLFDANPEILRAFAHTGISLTITVPNDQIPRLAKPNFAEDWIKFNVQPYVPATDIIRVLV 123
           RLFDANP+ILRAFAHTGIS++IT+PNDQIPRLAKPNFAE+WIKFN++PY+PAT+IIRVLV
Sbjct: 100 RLFDANPDILRAFAHTGISVSITIPNDQIPRLAKPNFAEEWIKFNIEPYIPATNIIRVLV 159

Query: 124 GNEVLATANKLLIANLVPAMQNLHTALAGASLDRRIQVSTPHSLGILSNSSPPSTGRFRQ 183
           GNEVL+TANKLLIANLVPAMQ+LHTAL  ASLDRRIQ+STPHSLGILSNSSPPSTGRFRQ
Sbjct: 160 GNEVLSTANKLLIANLVPAMQSLHTALTEASLDRRIQISTPHSLGILSNSSPPSTGRFRQ 219

Query: 184 GYDTHVLKPMLNFLRETNSAFMVNPYPFFGSSPETLDYALFRPNPGVSDPDSGLLYTNML 243
           GYDTHV+KPML+FLRETNS  MVNPYPFF  + E LDYALFRPNPGV D DSG+LYTNML
Sbjct: 220 GYDTHVMKPMLSFLRETNSPLMVNPYPFFACTAENLDYALFRPNPGVFDSDSGILYTNML 279

Query: 244 DAQLDAVFSAMKSLGFADVDIVIAETGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVG 303
           DAQLDAV+SAMKSLGF+D+DIVIAETGWPSKGD AQ+G+GPKEAAD+NGNLMRHVVSG G
Sbjct: 280 DAQLDAVYSAMKSLGFSDLDIVIAETGWPSKGDQAQIGVGPKEAADYNGNLMRHVVSGKG 339

Query: 304 TPLMPNRTFETYIFALFNEDLKPGPICERNFGLFEPDLSPVYEIGILRPTV-AATARTH- 363
           TPLMPNRTFETYIFALFNEDLKPGP+ ERNFGLFEPDLSPVYEIGILRPT  ++T + H 
Sbjct: 340 TPLMPNRTFETYIFALFNEDLKPGPVGERNFGLFEPDLSPVYEIGILRPTAQSSTPKAHQ 399

Query: 364 ---------------PKTPVTGGKKWCLPKTGADSEALQRNIDYVCGMGLDCGPIQENGP 423
                            +PV+ GK+WCLPKTGADSEALQRNIDYVCG+GLDCGPIQENGP
Sbjct: 400 GPIEGSVSTPRSPSPSPSPVSEGKRWCLPKTGADSEALQRNIDYVCGLGLDCGPIQENGP 459

Query: 424 CFAPNTVRAHAAYVMNAYFQATDGNDYDCDFDQTGALTTMDPS 445
           CFAPNTVRAHAAYVMNAYFQAT+GND DCDFDQTGALTT+DPS
Sbjct: 460 CFAPNTVRAHAAYVMNAYFQATEGNDSDCDFDQTGALTTVDPS 501

BLAST of MS006185 vs. NCBI nr
Match: XP_022958170.1 (glucan endo-1,3-beta-glucosidase [Cucurbita moschata])

HSP 1 Score: 759.2 bits (1959), Expect = 2.0e-215
Identity = 368/433 (84.99%), Postives = 400/433 (92.38%), Query Frame = 0

Query: 23  AKGGSIGVNYGTVADNLPPPSKVAQFLSDSTIINRVRLFDANPEILRAFAHTGISLTITV 82
           AKGGSIGVNYGT+ADNLP PSKVA+FL DSTII+R+RLFDANP+ILRAFAHT IS+TITV
Sbjct: 34  AKGGSIGVNYGTLADNLPLPSKVAEFLLDSTIIDRIRLFDANPDILRAFAHTRISVTITV 93

Query: 83  PNDQIPRLAKPNFAEDWIKFNVQPYVPATDIIRVLVGNEVLATANKLLIANLVPAMQNLH 142
           PNDQIPRL KPNFAE+WIKFNVQPYVPATDIIR+LVGNEVL+TANK+LIANLVPAMQ+LH
Sbjct: 94  PNDQIPRLVKPNFAEEWIKFNVQPYVPATDIIRILVGNEVLSTANKMLIANLVPAMQSLH 153

Query: 143 TALAGASLDRRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVLKPMLNFLRETNSAFMVN 202
           TAL+ ASL+RRIQVSTPHSLGILSNSSPPST RFRQGYDTHV+KPML+FLRETNS  MVN
Sbjct: 154 TALSEASLNRRIQVSTPHSLGILSNSSPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVN 213

Query: 203 PYPFFGSSPETLDYALFRPNPGVSDPDSGLLYTNMLDAQLDAVFSAMKSLGFADVDIVIA 262
           PYPFFG++ ETLDYALFRPNPGV D DSG+LYTNMLDAQLDAV+SAMKSLGFAD+DIVIA
Sbjct: 214 PYPFFGTTAETLDYALFRPNPGVYDSDSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIA 273

Query: 263 ETGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPG 322
           ETGWPSKGDPAQ+G+GPKEAAD+NGNL+RHVVSGVGTPLMPNRTFETYIFALFNE+LKPG
Sbjct: 274 ETGWPSKGDPAQIGVGPKEAADYNGNLLRHVVSGVGTPLMPNRTFETYIFALFNENLKPG 333

Query: 323 PICERNFGLFEPDLSPVYEIGILRPTV-AATARTH------PK----TPVTGGKKWCLPK 382
           P+ ERNFGLFEPDLSPVYEIGILRPT  ++T R H      PK    +PV  GK+WCLPK
Sbjct: 334 PVGERNFGLFEPDLSPVYEIGILRPTAQSSTPRAHHGPEPTPKGPSPSPVADGKRWCLPK 393

Query: 383 TGADSEALQRNIDYVCGMGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATDGNDYDCD 442
           T ADSEALQRNIDYVCG+GLDCGPIQENGPCF PNTVRAHAAYVMNAYFQATDGND DCD
Sbjct: 394 TEADSEALQRNIDYVCGLGLDCGPIQENGPCFLPNTVRAHAAYVMNAYFQATDGNDSDCD 453

Query: 443 FDQTGALTTMDPS 445
           F QTG LTT+DPS
Sbjct: 454 FQQTGDLTTVDPS 466

BLAST of MS006185 vs. NCBI nr
Match: XP_023521611.1 (glucan endo-1,3-beta-glucosidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 756.9 bits (1953), Expect = 1.0e-214
Identity = 368/435 (84.60%), Postives = 398/435 (91.49%), Query Frame = 0

Query: 23  AKGGSIGVNYGTVADNLPPPSKVAQFLSDSTIINRVRLFDANPEILRAFAHTGISLTITV 82
           AKGGSIGVNYGT+ADNLP PSKVA+FL DSTII+R+RLFDANP+ILRAFAHT IS+TITV
Sbjct: 49  AKGGSIGVNYGTLADNLPLPSKVAEFLLDSTIIDRIRLFDANPDILRAFAHTRISVTITV 108

Query: 83  PNDQIPRLAKPNFAEDWIKFNVQPYVPATDIIRVLVGNEVLATANKLLIANLVPAMQNLH 142
           PNDQIPRL KPNFAE+WIKFNVQPYVPATDIIR+LVGNEVL+TANK+LIANLVPAMQ+LH
Sbjct: 109 PNDQIPRLVKPNFAEEWIKFNVQPYVPATDIIRILVGNEVLSTANKMLIANLVPAMQSLH 168

Query: 143 TALAGASLDRRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVLKPMLNFLRETNSAFMVN 202
           TAL+ ASL RRIQVSTPHSLGILSNSSPPST RFRQGYDTHV+KPML+FLRETNS  MVN
Sbjct: 169 TALSEASLSRRIQVSTPHSLGILSNSSPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVN 228

Query: 203 PYPFFGSSPETLDYALFRPNPGVSDPDSGLLYTNMLDAQLDAVFSAMKSLGFADVDIVIA 262
           PYPFFG++ ETLDYALFRPNPGV D DSG+LYTNMLDAQLDAV+SAMKSLGFAD+DIVIA
Sbjct: 229 PYPFFGTTAETLDYALFRPNPGVYDSDSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIA 288

Query: 263 ETGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPG 322
           ETGWPSKGDPAQ+G+GPKEAAD+NGNL+RHVVSGVGTPLMPNRTFETYIFALFNE+LKPG
Sbjct: 289 ETGWPSKGDPAQIGVGPKEAADYNGNLLRHVVSGVGTPLMPNRTFETYIFALFNENLKPG 348

Query: 323 PICERNFGLFEPDLSPVYEIGILRPTV-AATARTH------PK------TPVTGGKKWCL 382
           P+ ERNFGLFEPDLSPVYEIGILRPT  ++T R H      PK      +PV  GK WCL
Sbjct: 349 PVGERNFGLFEPDLSPVYEIGILRPTAQSSTPRAHHGPEPTPKGPSPSPSPVADGKTWCL 408

Query: 383 PKTGADSEALQRNIDYVCGMGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATDGNDYD 442
           PKT ADSEALQRNIDYVCG+GLDCGPIQENGPCF PNTVRAHAAYVMNAYFQATDGND D
Sbjct: 409 PKTEADSEALQRNIDYVCGLGLDCGPIQENGPCFLPNTVRAHAAYVMNAYFQATDGNDSD 468

Query: 443 CDFDQTGALTTMDPS 445
           CDF QTG LTT+DPS
Sbjct: 469 CDFQQTGDLTTVDPS 483

BLAST of MS006185 vs. NCBI nr
Match: XP_023533272.1 (glucan endo-1,3-beta-glucosidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 756.9 bits (1953), Expect = 1.0e-214
Identity = 368/435 (84.60%), Postives = 398/435 (91.49%), Query Frame = 0

Query: 23  AKGGSIGVNYGTVADNLPPPSKVAQFLSDSTIINRVRLFDANPEILRAFAHTGISLTITV 82
           AKGGSIGVNYGT+ADNLP PSKVA+FL DSTII+R+RLFDANP+ILRAFAHT IS+TITV
Sbjct: 34  AKGGSIGVNYGTLADNLPLPSKVAEFLLDSTIIDRIRLFDANPDILRAFAHTRISVTITV 93

Query: 83  PNDQIPRLAKPNFAEDWIKFNVQPYVPATDIIRVLVGNEVLATANKLLIANLVPAMQNLH 142
           PNDQIPRL KPNFAE+WIKFNVQPYVPATDIIR+LVGNEVL+TANK+LIANLVPAMQ+LH
Sbjct: 94  PNDQIPRLVKPNFAEEWIKFNVQPYVPATDIIRILVGNEVLSTANKMLIANLVPAMQSLH 153

Query: 143 TALAGASLDRRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVLKPMLNFLRETNSAFMVN 202
           TAL+ ASL RRIQVSTPHSLGILSNSSPPST RFRQGYDTHV+KPML+FLRETNS  MVN
Sbjct: 154 TALSEASLSRRIQVSTPHSLGILSNSSPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVN 213

Query: 203 PYPFFGSSPETLDYALFRPNPGVSDPDSGLLYTNMLDAQLDAVFSAMKSLGFADVDIVIA 262
           PYPFFG++ ETLDYALFRPNPGV D DSG+LYTNMLDAQLDAV+SAMKSLGFAD+DIVIA
Sbjct: 214 PYPFFGTTAETLDYALFRPNPGVYDSDSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIA 273

Query: 263 ETGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPG 322
           ETGWPSKGDPAQ+G+GPKEAAD+NGNL+RHVVSGVGTPLMPNRTFETYIFALFNE+LKPG
Sbjct: 274 ETGWPSKGDPAQIGVGPKEAADYNGNLLRHVVSGVGTPLMPNRTFETYIFALFNENLKPG 333

Query: 323 PICERNFGLFEPDLSPVYEIGILRPTV-AATARTH------PK------TPVTGGKKWCL 382
           P+ ERNFGLFEPDLSPVYEIGILRPT  ++T R H      PK      +PV  GK WCL
Sbjct: 334 PVGERNFGLFEPDLSPVYEIGILRPTAQSSTPRAHHGPEPTPKGPSPSPSPVADGKTWCL 393

Query: 383 PKTGADSEALQRNIDYVCGMGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATDGNDYD 442
           PKT ADSEALQRNIDYVCG+GLDCGPIQENGPCF PNTVRAHAAYVMNAYFQATDGND D
Sbjct: 394 PKTEADSEALQRNIDYVCGLGLDCGPIQENGPCFLPNTVRAHAAYVMNAYFQATDGNDSD 453

Query: 443 CDFDQTGALTTMDPS 445
           CDF QTG LTT+DPS
Sbjct: 454 CDFQQTGDLTTVDPS 468

BLAST of MS006185 vs. NCBI nr
Match: KAG6605794.1 (Importin subunit beta-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 756.5 bits (1952), Expect = 1.3e-214
Identity = 367/435 (84.37%), Postives = 399/435 (91.72%), Query Frame = 0

Query: 23  AKGGSIGVNYGTVADNLPPPSKVAQFLSDSTIINRVRLFDANPEILRAFAHTGISLTITV 82
           AKGGSIGVNYGT+ADNLP PSKVA+FL DSTII+R+RLFDANP+ILRAFAHT IS+TITV
Sbjct: 34  AKGGSIGVNYGTLADNLPLPSKVAEFLLDSTIIDRIRLFDANPDILRAFAHTRISVTITV 93

Query: 83  PNDQIPRLAKPNFAEDWIKFNVQPYVPATDIIRVLVGNEVLATANKLLIANLVPAMQNLH 142
           PNDQIPRL KPNFAE+WIKFNVQPYVPATDIIR+LVGNEVL+TANK+LIANLVPAMQ+LH
Sbjct: 94  PNDQIPRLVKPNFAEEWIKFNVQPYVPATDIIRILVGNEVLSTANKMLIANLVPAMQSLH 153

Query: 143 TALAGASLDRRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVLKPMLNFLRETNSAFMVN 202
           TAL+ ASL+ RIQVSTPHSLGILSNSSPPST RFRQGYDTHV+KPML+FLRETNS  MVN
Sbjct: 154 TALSEASLNHRIQVSTPHSLGILSNSSPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVN 213

Query: 203 PYPFFGSSPETLDYALFRPNPGVSDPDSGLLYTNMLDAQLDAVFSAMKSLGFADVDIVIA 262
           PYPFFG++ ETLDYALFRPNPGV D DSG+LYTNMLDAQLDAV+SAMKSLGFAD+DIVIA
Sbjct: 214 PYPFFGTTAETLDYALFRPNPGVYDSDSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIA 273

Query: 263 ETGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPG 322
           ETGWPSKGDPAQ+G+GPKEAAD+NGNL+RHVVSGVGTPLMPNRTFETYIFALFNE+LKPG
Sbjct: 274 ETGWPSKGDPAQIGVGPKEAADYNGNLLRHVVSGVGTPLMPNRTFETYIFALFNENLKPG 333

Query: 323 PICERNFGLFEPDLSPVYEIGILRPTV-AATARTH------PK------TPVTGGKKWCL 382
           P+ ERNFGLFEPDLSPVYEIGILRPT  ++T R H      PK      +PV  GK+WCL
Sbjct: 334 PVGERNFGLFEPDLSPVYEIGILRPTAQSSTPRAHHGPEPTPKGPSPRPSPVADGKRWCL 393

Query: 383 PKTGADSEALQRNIDYVCGMGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATDGNDYD 442
           PKT ADSEALQRNIDYVCG+GLDCGPIQENGPCF PNTVRAHAAYVMNAYFQATDGND D
Sbjct: 394 PKTEADSEALQRNIDYVCGLGLDCGPIQENGPCFLPNTVRAHAAYVMNAYFQATDGNDSD 453

Query: 443 CDFDQTGALTTMDPS 445
           CDF QTG LTT+DPS
Sbjct: 454 CDFQQTGDLTTVDPS 468

BLAST of MS006185 vs. ExPASy Swiss-Prot
Match: P52409 (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 1.7e-129
Identity = 232/449 (51.67%), Postives = 304/449 (67.71%), Query Frame = 0

Query: 16  LHYLCSGAKGGS-------IGVNYGTVADNLPPPSKVAQFLSDSTIINRVRLFDANPEIL 75
           L  L +GA G         IGVNYG  ADNLP P+ VA FL+  T I+RV+LFDANP  +
Sbjct: 7   LMLLAAGAAGAESATPSLHIGVNYGANADNLPSPTSVATFLATKTTIDRVKLFDANPTFI 66

Query: 76  RAFAHTGISLTITVPNDQIPRLAKP----NFAEDWIKFNVQPYVPATDIIRVLVGNEVLA 135
            AFA T ISL +++PN  +P LA      + A  WI+ N+ PYVPAT++  +L GNE+L 
Sbjct: 67  SAFAGTPISLAVSLPNSALPALADKATGLDAARSWIRANLSPYVPATNVTLLLAGNEILL 126

Query: 136 TANKLLIANLVPAMQNLHTALAGASLDRRIQVSTPHSLGILSNSSP-PSTGRFRQGYDTH 195
           + +  LI +L+PAM+ L  AL    L   ++V+TPH LGIL+ S   PS   FR GY+T 
Sbjct: 127 STDTNLILSLLPAMRRLAQALKAEGL-TGVRVTTPHYLGILAPSDGIPSNASFRAGYNTK 186

Query: 196 VLKPMLNFLRETNSAFMVNPYPFFGSSPETLDYALFRPNPGVSDPDSGLLYTNMLDAQLD 255
           +   ML F R+T S FMVNPYP+F   PETL+YALFRPN G+ DP + L YT+MLDAQ+D
Sbjct: 187 LFPAMLQFHRDTGSPFMVNPYPYFSYRPETLNYALFRPNSGIYDPATKLNYTSMLDAQMD 246

Query: 256 AVFSAMKSLGFADVDIVIAETGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVGTPLMP 315
           A+++AMK LG+ DVDI + E GWP++ +P Q+G+G +EA DFN  ++R   SG GTPLMP
Sbjct: 247 AIYTAMKKLGYGDVDIAVGEAGWPTQAEPGQIGVGVQEARDFNEGMIRVCSSGKGTPLMP 306

Query: 316 NRTFETYIFALFNEDLKPGPICERNFGLFEPDLSPVYEIGILR--------PTVAATART 375
           NRTFETY+F+LF+E+ KPGPI ER+FGLF PD +PVY++G+LR        P+   +   
Sbjct: 307 NRTFETYLFSLFDENQKPGPIAERHFGLFNPDFTPVYDLGLLRDGASVAPTPSPNPSPNP 366

Query: 376 HPKTPVTGGKKWCLPKTGADSEALQRNIDYVCGMGLDCGPIQENGPCFAPNTVRAHAAYV 435
            PK   +GG KWC+ K GA+   LQ NI+Y CG  +DC PIQ  G CF+PN+++AHA+YV
Sbjct: 367 SPKPAPSGGGKWCVAKDGANGTDLQNNINYACGF-VDCKPIQSGGACFSPNSLQAHASYV 426

Query: 436 MNAYFQATDGNDYDCDFDQTGALTTMDPS 445
           MNAY+QA    D  CDF  TG +T+ DPS
Sbjct: 427 MNAYYQANGHTDLACDFKGTGIVTSSDPS 453

BLAST of MS006185 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 419.5 bits (1077), Expect = 4.9e-116
Identity = 215/435 (49.43%), Postives = 285/435 (65.52%), Query Frame = 0

Query: 15  FLHYLCSGAKGGSIGVNYGTVADNLPPPSKVAQFLSDSTIINRVRLFDANPEILRAFAHT 74
           FL +  S      IGVNYG VADNLPPPS+  + L  ST I +VRL+ A+P I++A A T
Sbjct: 13  FLSHFPSSHAEPFIGVNYGQVADNLPPPSETVKLL-QSTSIQKVRLYGADPAIIKALAGT 72

Query: 75  GISLTITVPNDQIPRLAK-PNFAEDWIKFNVQPYVPATDIIRVLVGNEVLATANKLLIAN 134
           G+ + I   N  +P LA  PN A  WI  NV P+ PA+ I+ + VGNE+L + +  L+  
Sbjct: 73  GVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQ 132

Query: 135 LVPAMQNLHTALAGASLDRRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVLKPMLNFLR 194
           L+PAMQN+  AL   SL  +I+VST +S+ +L +S PPS+G F  GY T  LK +L FL 
Sbjct: 133 LLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTG-LKGILQFLS 192

Query: 195 ETNSAFMVNPYPFFG----SSPETLDYALFRPNPGVSDPDSGLLYTNMLDAQLDAVFSAM 254
           +T S F +NPYPFF       PETL + LF PN G  D  +G+ YTNM DAQ+DAV SA+
Sbjct: 193 DTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSAL 252

Query: 255 KSLGFADVDIVIAETGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVGTPLMPNRTFET 314
           KS+GF  V+IV+AETGW S+GD  +VG     A  +NGNL+ H+ S VGTPLMP +  +T
Sbjct: 253 KSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDT 312

Query: 315 YIFALFNEDLKPGPICERNFGLFEPDLSPVYEIGILRPTVAATARTHPKTPVTGGKKWCL 374
           YIFAL++E+LKPGP  ER FGLF+ DLS VY++G+ +   ++++   P   VT    WC+
Sbjct: 313 YIFALYDENLKPGPSSERAFGLFKTDLSMVYDVGLAK---SSSSSQTPSGKVT-SSGWCV 372

Query: 375 PKTGADSEALQRNIDYVCGMGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATDGNDYD 434
           PK GA +E LQ ++D+ CG G+DCG IQ  G CF PN V +HAAY MN YFQ +     D
Sbjct: 373 PKKGATNEELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAMNMYFQKSPKQPTD 432

Query: 435 CDFDQTGALTTMDPS 445
           CDF +T  +T+ +PS
Sbjct: 433 CDFSKTATVTSQNPS 441

BLAST of MS006185 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 3.3e-112
Identity = 198/422 (46.92%), Postives = 273/422 (64.69%), Query Frame = 0

Query: 28  IGVNYGTVADNLPPPSKVAQFLSDSTIINRVRLFDANPEILRAFAHTGISLTITVPNDQI 87
           +GVNYG ++DNLP        L  ST I +VRLF A P +++AFA+TG+ + I   N  I
Sbjct: 30  LGVNYGQLSDNLPSLQATVNLLK-STTIQKVRLFGAEPAVIKAFANTGVEIVIGFDNGDI 89

Query: 88  PRLA-KPNFAEDWIKFNVQPYVPATDIIRVLVGNEVLATANKLLIANLVPAMQNLHTALA 147
           P LA  PN A  ++K NV  + PA++II + VGNEVL + ++ LI+ L+PAMQN+  AL 
Sbjct: 90  PTLASNPNVASQFVKSNVMSFYPASNIIAITVGNEVLTSGDQKLISQLLPAMQNVQNALN 149

Query: 148 GASLDRRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVLKPMLNFLRETNSAFMVNPYPF 207
            ASL  +++VST H++ +LS S PPS+G F  G     +K +L F    ++ FM++PYP+
Sbjct: 150 AASLGGKVKVSTVHAMAVLSQSYPPSSGVFNPGLG-DTMKALLQFQSANDAPFMISPYPY 209

Query: 208 FG----SSPETLDYALFRPNPGVSDPDSGLLYTNMLDAQLDAVFSAMKSLGFADVDIVIA 267
           F      +P+TL + LF+PN G  D  +G  YTNM DAQ+DAV SA+ ++GF D++IV+A
Sbjct: 210 FAYKNQPTPDTLAFCLFQPNAGQVDSGNGHKYTNMFDAQVDAVHSALNAMGFKDIEIVVA 269

Query: 268 ETGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPG 327
           ETGWP  GD  +VG     A  + GNL+ H+ S VGTPLMP ++ +TY+F+L++ED K G
Sbjct: 270 ETGWPHGGDSNEVGPSLDNAKAYVGNLINHLKSKVGTPLMPGKSIDTYLFSLYDEDKKTG 329

Query: 328 PICERNFGLFEPDLSPVYEIGILRPTVAATARTHPKTPVTGGKKWCLPKTGADSEALQRN 387
              E+ FGLF+PD S  Y++G+L+ T   T    P         WC+PK G   + L  N
Sbjct: 330 ASSEKYFGLFKPDGSTTYDVGLLKNTQNPTTPATPTPTPKAAGSWCVPKPGVSDDQLTGN 389

Query: 388 IDYVCGMGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATDGNDYDCDFDQTGALTTMD 445
           I+Y CG G+DCGPIQ  G CF PNTV+AHAAYVMN Y+Q+   N ++CDF QT  LT  +
Sbjct: 390 INYACGQGIDCGPIQPGGACFEPNTVKAHAAYVMNLYYQSAGRNSWNCDFSQTATLTNTN 449

BLAST of MS006185 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 3.9e-89
Identity = 177/427 (41.45%), Postives = 245/427 (57.38%), Query Frame = 0

Query: 25  GGSIGVNYGTVADNLPPPSKVAQFLSDSTIINRVRLFDANPEILRAFAHTGISLTITVPN 84
           GG +GV YG  AD+LP PSKV Q +     I  VR++D N ++L+AF +T I L I VPN
Sbjct: 23  GGKVGVCYGRSADDLPTPSKVVQLIQQHN-IKYVRIYDYNSQVLKAFGNTSIELMIGVPN 82

Query: 85  DQIPRLAKPNFAED-WIKFNVQPYVPATDIIRVLVGNEVLATANKLLIANLVPAMQNLHT 144
             +   ++     D W+K +V PY P T I  + VG E     +    + +VPAMQN+ T
Sbjct: 83  SDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDDPHINASSFVVPAMQNVLT 142

Query: 145 ALAGASLDRRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVLKPMLNFLRETNSAFMVNP 204
           AL    L RRI+VST  SLGILS S PPS G F   Y  + L+PML FL E  S FM++ 
Sbjct: 143 ALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSY-AYFLRPMLEFLAENKSPFMIDL 202

Query: 205 YPFFG--SSPE--TLDYALFRPNPGVSDPDSGLLYTNMLDAQLDAVFSAMKSLGFADVDI 264
           YP++    SP   +LDY LF  +  V DP++GLLY NM DAQ+DA++ A+ +L F  + I
Sbjct: 203 YPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQVDALYYALTALNFRTIKI 262

Query: 265 VIAETGWPSKGDPAQVGIGPKEAAD-FNGNLMRHVVSGVGTPLMPNRTFETYIFALFNED 324
           ++ ETGWP+KG P +      + A+ +N N++RHVV+  GTP  P      YIF+LFNE+
Sbjct: 263 MVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAKPGEAMNVYIFSLFNEN 322

Query: 325 LKPGPICERNFGLFEPDLSPVYEIGILRPTVAATARTHPKTPVTGGKKWCLPKTGADSEA 384
            K G   ERN+GLF PD + VY++     +    + +           WC+  + A    
Sbjct: 323 RKAGLDSERNWGLFYPDQTSVYQLDFTGKSNGFHSNSSGTNSSGSSNSWCIASSKASERD 382

Query: 385 LQRNIDYVCGMG-LDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATDGNDYDCDFDQTGA 444
           L+  +D+ CG G +DC  IQ + PCF P+T+ +HA++V N+YFQ     D  C F   G 
Sbjct: 383 LKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFVFNSYFQQNRATDVACSFGGAGV 442

BLAST of MS006185 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 2.0e-88
Identity = 174/446 (39.01%), Postives = 258/446 (57.85%), Query Frame = 0

Query: 28  IGVNYGTVADNLPPPSKVAQFLSDSTIINRVRLFDANPEILRAFAHTGISLTITVPNDQI 87
           IG+ YG  ADNLP P++V++ +     I  VR++DAN ++L+AFA+TGI L I VPN  +
Sbjct: 27  IGICYGRNADNLPSPNRVSELIQHLN-IKFVRIYDANIDVLKAFANTGIELMIGVPNADL 86

Query: 88  PRLAK-PNFAEDWIKFNVQPYVPATDIIRVLVGNEVLATANKLLIANLVPAMQNLHTALA 147
              A+  +  + W+  N+ PY P+T I  + VG EV    +      ++PAM+N+HTAL 
Sbjct: 87  LAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDN-ATGLVLPAMRNIHTALK 146

Query: 148 GASLDRRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVLKPMLNFLRETNSAFMVNPYPF 207
            + LD++I++S+ HSL ILS S PPS+  F + +    LKPML FL E  S FM++ YP+
Sbjct: 147 KSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHSA-FLKPMLEFLVENESPFMIDLYPY 206

Query: 208 FGSSPET----LDYALFRPNPGVSDPDSGLLYTNMLDAQLDAVFSAMKSLGFADVDIVIA 267
           +     T    L+YALF  +  V DP +GLLY+NM DAQLDA++ A+ ++ F  V +++ 
Sbjct: 207 YAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFALTAMSFKTVKVMVT 266

Query: 268 ETGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPG 327
           E+GWPSKG P +    P+ A  +N NL+RHV+   GTP  P    + Y+F+LFNE+ KPG
Sbjct: 267 ESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEIDVYLFSLFNENRKPG 326

Query: 328 PICERNFGLFEPDLSPVYEIGI-------LRPT--VAATARTHPKTPVTGG--------- 387
              ERN+G+F  + + VY +         + PT     T+ +   +P+  G         
Sbjct: 327 IESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPSSSPIINGNSTVTIGGG 386

Query: 388 -----KKWCLPKTGADSEALQRNIDYVCGMG-LDCGPIQENGPCFAPNTVRAHAAYVMNA 445
                KKWC+  + A    LQ  +D+ CG G +DC  +Q + PCF P+TV +HA+Y  N 
Sbjct: 387 GGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYAFNT 446

BLAST of MS006185 vs. ExPASy TrEMBL
Match: A0A6J1H2P3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111459476 PE=3 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 9.7e-216
Identity = 368/433 (84.99%), Postives = 400/433 (92.38%), Query Frame = 0

Query: 23  AKGGSIGVNYGTVADNLPPPSKVAQFLSDSTIINRVRLFDANPEILRAFAHTGISLTITV 82
           AKGGSIGVNYGT+ADNLP PSKVA+FL DSTII+R+RLFDANP+ILRAFAHT IS+TITV
Sbjct: 34  AKGGSIGVNYGTLADNLPLPSKVAEFLLDSTIIDRIRLFDANPDILRAFAHTRISVTITV 93

Query: 83  PNDQIPRLAKPNFAEDWIKFNVQPYVPATDIIRVLVGNEVLATANKLLIANLVPAMQNLH 142
           PNDQIPRL KPNFAE+WIKFNVQPYVPATDIIR+LVGNEVL+TANK+LIANLVPAMQ+LH
Sbjct: 94  PNDQIPRLVKPNFAEEWIKFNVQPYVPATDIIRILVGNEVLSTANKMLIANLVPAMQSLH 153

Query: 143 TALAGASLDRRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVLKPMLNFLRETNSAFMVN 202
           TAL+ ASL+RRIQVSTPHSLGILSNSSPPST RFRQGYDTHV+KPML+FLRETNS  MVN
Sbjct: 154 TALSEASLNRRIQVSTPHSLGILSNSSPPSTARFRQGYDTHVIKPMLSFLRETNSPLMVN 213

Query: 203 PYPFFGSSPETLDYALFRPNPGVSDPDSGLLYTNMLDAQLDAVFSAMKSLGFADVDIVIA 262
           PYPFFG++ ETLDYALFRPNPGV D DSG+LYTNMLDAQLDAV+SAMKSLGFAD+DIVIA
Sbjct: 214 PYPFFGTTAETLDYALFRPNPGVYDSDSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIA 273

Query: 263 ETGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPG 322
           ETGWPSKGDPAQ+G+GPKEAAD+NGNL+RHVVSGVGTPLMPNRTFETYIFALFNE+LKPG
Sbjct: 274 ETGWPSKGDPAQIGVGPKEAADYNGNLLRHVVSGVGTPLMPNRTFETYIFALFNENLKPG 333

Query: 323 PICERNFGLFEPDLSPVYEIGILRPTV-AATARTH------PK----TPVTGGKKWCLPK 382
           P+ ERNFGLFEPDLSPVYEIGILRPT  ++T R H      PK    +PV  GK+WCLPK
Sbjct: 334 PVGERNFGLFEPDLSPVYEIGILRPTAQSSTPRAHHGPEPTPKGPSPSPVADGKRWCLPK 393

Query: 383 TGADSEALQRNIDYVCGMGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATDGNDYDCD 442
           T ADSEALQRNIDYVCG+GLDCGPIQENGPCF PNTVRAHAAYVMNAYFQATDGND DCD
Sbjct: 394 TEADSEALQRNIDYVCGLGLDCGPIQENGPCFLPNTVRAHAAYVMNAYFQATDGNDSDCD 453

Query: 443 FDQTGALTTMDPS 445
           F QTG LTT+DPS
Sbjct: 454 FQQTGDLTTVDPS 466

BLAST of MS006185 vs. ExPASy TrEMBL
Match: A0A6J1K780 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111491291 PE=3 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 5.9e-213
Identity = 363/433 (83.83%), Postives = 395/433 (91.22%), Query Frame = 0

Query: 23  AKGGSIGVNYGTVADNLPPPSKVAQFLSDSTIINRVRLFDANPEILRAFAHTGISLTITV 82
           AKGGSIGVNYGT+ADNLP PSKVA+FL DSTII R+RLFDANP+ILRAFAHT IS+TITV
Sbjct: 72  AKGGSIGVNYGTLADNLPLPSKVAEFLLDSTIIGRIRLFDANPDILRAFAHTRISVTITV 131

Query: 83  PNDQIPRLAKPNFAEDWIKFNVQPYVPATDIIRVLVGNEVLATANKLLIANLVPAMQNLH 142
           PNDQIPRL KPNFAE+WIKFNVQPYVPATDIIR+LVGNEVL+TANK+LIANLVPAMQ+LH
Sbjct: 132 PNDQIPRLVKPNFAEEWIKFNVQPYVPATDIIRILVGNEVLSTANKMLIANLVPAMQSLH 191

Query: 143 TALAGASLDRRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVLKPMLNFLRETNSAFMVN 202
           TAL+ ASL+ RIQVSTPHSLGILSNSSPPST RFRQGYDTHV+KPML+FL+ETNS  MVN
Sbjct: 192 TALSEASLNSRIQVSTPHSLGILSNSSPPSTARFRQGYDTHVIKPMLSFLKETNSPLMVN 251

Query: 203 PYPFFGSSPETLDYALFRPNPGVSDPDSGLLYTNMLDAQLDAVFSAMKSLGFADVDIVIA 262
           PYPFFG++ ETLDYALFRPNPGV D DSG+LYTNMLDAQLDAV+SAMKSLGFAD+DIVIA
Sbjct: 252 PYPFFGTTAETLDYALFRPNPGVYDSDSGILYTNMLDAQLDAVYSAMKSLGFADLDIVIA 311

Query: 263 ETGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPG 322
           ETGWPSKGDPAQ+G+GPKEAAD+NGNL+RHVVSGVGTPLMPNRTFETYIFALFNEDLKPG
Sbjct: 312 ETGWPSKGDPAQIGVGPKEAADYNGNLLRHVVSGVGTPLMPNRTFETYIFALFNEDLKPG 371

Query: 323 PICERNFGLFEPDLSPVYEIGILRPTV-AATARTH------PK----TPVTGGKKWCLPK 382
           P+ ERNFGLFEPDL+PVYEIGILRPT  ++T R H      PK    +PV  GK+WCLPK
Sbjct: 372 PVGERNFGLFEPDLNPVYEIGILRPTAQSSTPRAHHGPEPTPKGPSPSPVADGKRWCLPK 431

Query: 383 TGADSEALQRNIDYVCGMGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATDGNDYDCD 442
           T ADSEALQRNIDYVCG+G DCGPIQ NGPCF PNTVRAHAAYVMNAYFQA DGND DCD
Sbjct: 432 TEADSEALQRNIDYVCGLGFDCGPIQANGPCFLPNTVRAHAAYVMNAYFQAIDGNDSDCD 491

Query: 443 FDQTGALTTMDPS 445
           F QTG LTT+DPS
Sbjct: 492 FQQTGDLTTVDPS 504

BLAST of MS006185 vs. ExPASy TrEMBL
Match: A0A1S3AV36 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103482986 PE=3 SV=1)

HSP 1 Score: 733.0 bits (1891), Expect = 7.5e-208
Identity = 352/438 (80.37%), Postives = 392/438 (89.50%), Query Frame = 0

Query: 22  GAKGGSIGVNYGTVADNLPPPSKVAQFLSDSTIINRVRLFDANPEILRAFAHTGISLTIT 81
           G  GGSIGVNYGTVADNLPPPSKVA FL D+TII+RVRLFDA+P+ILRAFAHTGIS++IT
Sbjct: 55  GVNGGSIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSIT 114

Query: 82  VPNDQIPRLAKPNFAEDWIKFNVQPYVPATDIIRVLVGNEVLATANKLLIANLVPAMQNL 141
           +PNDQIPRL KPNFAE+WIKFN+QPY+PAT+IIRVLVGNEVL+TANKLLIANLVPAMQ+L
Sbjct: 115 IPNDQIPRLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSL 174

Query: 142 HTALAGASLDRRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVLKPMLNFLRETNSAFMV 201
           HTAL  ASLDRRIQ+STPHSLGILSNS+PPSTGRFRQGYDTHV+KPML+FLRETNS  MV
Sbjct: 175 HTALVEASLDRRIQISTPHSLGILSNSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMV 234

Query: 202 NPYPFFGSSPETLDYALFRPNPGVSDPDSGLLYTNMLDAQLDAVFSAMKSLGFADVDIVI 261
           NPYPFF  + E LDYALFRPNPGV D D G+LYTNMLDAQLDAV+SAMKS+ F D+DIVI
Sbjct: 235 NPYPFFACTAENLDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSMNFEDLDIVI 294

Query: 262 AETGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKP 321
           AETGWPSKGDP+QVG+GPKEAA +NGNLMRHVVSG GTPLMPNRTFETYIFALFNE+LKP
Sbjct: 295 AETGWPSKGDPSQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKP 354

Query: 322 GPICERNFGLFEPDLSPVYEIGILRPTV-AATARTH----------PKTP----VTGGKK 381
           GP+ ERNFGLFEPDL+PVYEIGILRPT  +AT R H          P++P    V+  K+
Sbjct: 355 GPVGERNFGLFEPDLTPVYEIGILRPTAQSATPRAHQGPVEGPISTPRSPSRSTVSESKR 414

Query: 382 WCLPKTGADSEALQRNIDYVCGMGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATDGN 441
           WC+PKT A  EALQRNIDYVCG+GLDCGPIQENGPC+APNTVR HAAYVMNAYFQAT+G 
Sbjct: 415 WCVPKTEASVEALQRNIDYVCGLGLDCGPIQENGPCYAPNTVRGHAAYVMNAYFQATEGK 474

Query: 442 DYDCDFDQTGALTTMDPS 445
           ++DCDFDQTGALTT+DPS
Sbjct: 475 EFDCDFDQTGALTTVDPS 492

BLAST of MS006185 vs. ExPASy TrEMBL
Match: A0A0A0KJS0 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G139170 PE=3 SV=1)

HSP 1 Score: 732.6 bits (1890), Expect = 9.7e-208
Identity = 354/432 (81.94%), Postives = 388/432 (89.81%), Query Frame = 0

Query: 22  GAKGGSIGVNYGTVADNLPPPSKVAQFLSDSTIINRVRLFDANPEILRAFAHTGISLTIT 81
           G  GGSIGVNYGTVADNLPPPSKVA FL D+TII+RVRLFDA+P+ILRAFAHTGIS++IT
Sbjct: 56  GVNGGSIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSIT 115

Query: 82  VPNDQIPRLAKPNFAEDWIKFNVQPYVPATDIIRVLVGNEVLATANKLLIANLVPAMQNL 141
           +PNDQIPRL KPNFAE+WIKFN+QPY+PAT+IIRVLVGNEVL+TANKLLIANLVPAMQ+L
Sbjct: 116 IPNDQIPRLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSL 175

Query: 142 HTALAGASLDRRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVLKPMLNFLRETNSAFMV 201
           HTAL  ASLDRRIQ+STPHSLGILSNS+PPST RFRQGYDTHV+KPML+FLRETNS  MV
Sbjct: 176 HTALIEASLDRRIQISTPHSLGILSNSTPPSTARFRQGYDTHVIKPMLSFLRETNSPLMV 235

Query: 202 NPYPFFGSSPETLDYALFRPNPGVSDPDSGLLYTNMLDAQLDAVFSAMKSLGFADVDIVI 261
           NPYPFF  + + LDYALFRPNPGV D D G+LYTNMLDAQLDAV+SAMKSLGF D+DIVI
Sbjct: 236 NPYPFFACTADNLDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSLGFEDLDIVI 295

Query: 262 AETGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKP 321
           AETGWPSKGDP QVG+GPKEAA +NGNLMRHVVSG GTPLMPNRTFETYIFALFNE+LKP
Sbjct: 296 AETGWPSKGDPTQVGVGPKEAAYYNGNLMRHVVSGKGTPLMPNRTFETYIFALFNENLKP 355

Query: 322 GPICERNFGLFEPDLSPVYEIGILRPTV-AATARTH--------PKTPVTGGKKWCLPKT 381
           GPI ERNFGLFEPDLSPVYEIGILRPT  +AT R H        P T V+  K+WCLPK+
Sbjct: 356 GPIGERNFGLFEPDLSPVYEIGILRPTAQSATPRAHQGQVEGPSPST-VSESKRWCLPKS 415

Query: 382 GADSEALQRNIDYVCGMGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATDGNDYDCDF 441
            A  E LQRNIDYVCG+GLDCGPI+ENG CFAPNTVRAHAAYVMNAYFQAT+GND+DCDF
Sbjct: 416 EASEEGLQRNIDYVCGLGLDCGPIKENGACFAPNTVRAHAAYVMNAYFQATEGNDFDCDF 475

Query: 442 DQTGALTTMDPS 445
           DQTG LTT+DPS
Sbjct: 476 DQTGTLTTVDPS 486

BLAST of MS006185 vs. ExPASy TrEMBL
Match: A0A5A7TGS6 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00250 PE=3 SV=1)

HSP 1 Score: 730.3 bits (1884), Expect = 4.8e-207
Identity = 350/437 (80.09%), Postives = 391/437 (89.47%), Query Frame = 0

Query: 22  GAKGGSIGVNYGTVADNLPPPSKVAQFLSDSTIINRVRLFDANPEILRAFAHTGISLTIT 81
           G  GGSIGVNYGTVADNLPPPSKVA FL D+TII+RVRLFDA+P+ILRAFAHTGIS++IT
Sbjct: 36  GVNGGSIGVNYGTVADNLPPPSKVAAFLLDNTIIDRVRLFDADPDILRAFAHTGISVSIT 95

Query: 82  VPNDQIPRLAKPNFAEDWIKFNVQPYVPATDIIRVLVGNEVLATANKLLIANLVPAMQNL 141
           +PNDQIPRL KPNFAE+WIKFN+QPY+PAT+IIRVLVGNEVL+TANKLLIANLVPAMQ+L
Sbjct: 96  IPNDQIPRLVKPNFAEEWIKFNIQPYIPATNIIRVLVGNEVLSTANKLLIANLVPAMQSL 155

Query: 142 HTALAGASLDRRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVLKPMLNFLRETNSAFMV 201
           HTAL  ASLDRRIQ+STPHSLGILSNS+PPSTGRFRQGYDTHV+KPML+FLRETNS  MV
Sbjct: 156 HTALVEASLDRRIQISTPHSLGILSNSTPPSTGRFRQGYDTHVIKPMLSFLRETNSPLMV 215

Query: 202 NPYPFFGSSPETLDYALFRPNPGVSDPDSGLLYTNMLDAQLDAVFSAMKSLGFADVDIVI 261
           NPYPFF  + E LDYALFRPNPGV D D G+LYTNMLDAQLDAV+SAMKS+ F D+DIVI
Sbjct: 216 NPYPFFACTAENLDYALFRPNPGVFDSDLGILYTNMLDAQLDAVYSAMKSMNFEDLDIVI 275

Query: 262 AETGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKP 321
           AETGWPSKGDP+QVG+GPKEAA +NGNLMRHVV+G GTPLMPNRTFETYIFALFNE+LKP
Sbjct: 276 AETGWPSKGDPSQVGVGPKEAAYYNGNLMRHVVTGKGTPLMPNRTFETYIFALFNENLKP 335

Query: 322 GPICERNFGLFEPDLSPVYEIGILRPTV-AATARTH----------PKTP----VTGGKK 381
           GP+ ERNFGLFEPDL+PVYEIGILRPT  +AT R H          P++P    V+  K+
Sbjct: 336 GPVGERNFGLFEPDLTPVYEIGILRPTAQSATPRAHQGPVEGPISTPRSPSRSTVSESKR 395

Query: 382 WCLPKTGADSEALQRNIDYVCGMGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATDGN 441
           WC+PKT A  EALQRNIDYVCG+GLDCGPIQENGPC+APNTVR HAAYVMNAYFQAT+G 
Sbjct: 396 WCVPKTEASVEALQRNIDYVCGLGLDCGPIQENGPCYAPNTVRGHAAYVMNAYFQATEGK 455

Query: 442 DYDCDFDQTGALTTMDP 444
           ++DCDFDQTGALTT+DP
Sbjct: 456 EFDCDFDQTGALTTVDP 472

BLAST of MS006185 vs. TAIR 10
Match: AT5G24318.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 582.0 bits (1499), Expect = 4.1e-166
Identity = 275/434 (63.36%), Postives = 351/434 (80.88%), Query Frame = 0

Query: 14  GFLHYLCSGAKGGSIGVNYGTVADNLPPPSKVAQFLSDSTIINRVRLFDANPEILRAFAH 73
           G L  L +G++  SIGVNYGT+A+NLPPP +VA+FL  ST+INR+RLFD +P+IL+AFA+
Sbjct: 17  GVLLILSTGSE-ASIGVNYGTLANNLPPPQQVAEFLLHSTVINRIRLFDTDPQILQAFAN 76

Query: 74  TGISLTITVPNDQIPRLAKPNFAEDWIKFNVQPYVPATDIIRVLVGNEVLATANKLLIAN 133
           TGI++T+TVPNDQIP L   + A+ WI  ++QP+ P+T+IIR+LVGNEV++TA+ LLI  
Sbjct: 77  TGIAVTVTVPNDQIPHLTNLSSAKQWISDHIQPHFPSTNIIRILVGNEVISTADHLLIRT 136

Query: 134 LVPAMQNLHTALAGASLDRRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVLKPMLNFLR 193
           L+PAMQ+LHTAL  ASL RRIQ+STPHSLG L+NS+PPS+ +FR+GYD  VLKP+L+FLR
Sbjct: 137 LIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRRGYDAQVLKPLLSFLR 196

Query: 194 ETNSAFMVNPYPFFGSSPETLDYALFRPNPGVSDPDSGLLYTNMLDAQLDAVFSAMKSLG 253
            T+S F+VNPYPFFG S ETLD+ALFRPNPG+ D  + LLYTNMLDAQLD+V+SAM  LG
Sbjct: 197 STSSPFVVNPYPFFGYSIETLDFALFRPNPGLFDQHTKLLYTNMLDAQLDSVYSAMDKLG 256

Query: 254 FADVDIVIAETGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVGTPLMPNRTFETYIFA 313
           F+DV+IVI E GWPS+GD  Q+G+    AA+FN NL+  V SG GTPLMPNRTFETYIFA
Sbjct: 257 FSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTGTPLMPNRTFETYIFA 316

Query: 314 LFNEDLKPGPICERNFGLFEPDLSPVYEIGILRPTVAATARTH-PKTPVTG--GKKWCLP 373
           LFNE+LK GP  ERNFG+F  DL+P+Y+IGILRPT  ++   + P++PV G   K+WC+ 
Sbjct: 317 LFNENLKSGPTSERNFGIFRSDLTPIYDIGILRPTFRSSDPVYNPRSPVRGSSSKRWCVT 376

Query: 374 KTGADSEALQRNIDYVCGMGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATDGNDYDC 433
           K GA++ ALQRNIDYVCG+GLDC PI E G C+ PNTV+AH+ Y MN Y+Q  + +++DC
Sbjct: 377 KAGAETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTVKAHSKYAMNLYYQTMEKHEFDC 436

Query: 434 DFDQTGALTTMDPS 445
           DFD TG +TT+DPS
Sbjct: 437 DFDNTGEITTIDPS 449

BLAST of MS006185 vs. TAIR 10
Match: AT5G24318.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 582.0 bits (1499), Expect = 4.1e-166
Identity = 275/434 (63.36%), Postives = 351/434 (80.88%), Query Frame = 0

Query: 14  GFLHYLCSGAKGGSIGVNYGTVADNLPPPSKVAQFLSDSTIINRVRLFDANPEILRAFAH 73
           G L  L +G++  SIGVNYGT+A+NLPPP +VA+FL  ST+INR+RLFD +P+IL+AFA+
Sbjct: 17  GVLLILSTGSE-ASIGVNYGTLANNLPPPQQVAEFLLHSTVINRIRLFDTDPQILQAFAN 76

Query: 74  TGISLTITVPNDQIPRLAKPNFAEDWIKFNVQPYVPATDIIRVLVGNEVLATANKLLIAN 133
           TGI++T+TVPNDQIP L   + A+ WI  ++QP+ P+T+IIR+LVGNEV++TA+ LLI  
Sbjct: 77  TGIAVTVTVPNDQIPHLTNLSSAKQWISDHIQPHFPSTNIIRILVGNEVISTADHLLIRT 136

Query: 134 LVPAMQNLHTALAGASLDRRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVLKPMLNFLR 193
           L+PAMQ+LHTAL  ASL RRIQ+STPHSLG L+NS+PPS+ +FR+GYD  VLKP+L+FLR
Sbjct: 137 LIPAMQSLHTALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRRGYDAQVLKPLLSFLR 196

Query: 194 ETNSAFMVNPYPFFGSSPETLDYALFRPNPGVSDPDSGLLYTNMLDAQLDAVFSAMKSLG 253
            T+S F+VNPYPFFG S ETLD+ALFRPNPG+ D  + LLYTNMLDAQLD+V+SAM  LG
Sbjct: 197 STSSPFVVNPYPFFGYSIETLDFALFRPNPGLFDQHTKLLYTNMLDAQLDSVYSAMDKLG 256

Query: 254 FADVDIVIAETGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVGTPLMPNRTFETYIFA 313
           F+DV+IVI E GWPS+GD  Q+G+    AA+FN NL+  V SG GTPLMPNRTFETYIFA
Sbjct: 257 FSDVEIVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTGTPLMPNRTFETYIFA 316

Query: 314 LFNEDLKPGPICERNFGLFEPDLSPVYEIGILRPTVAATARTH-PKTPVTG--GKKWCLP 373
           LFNE+LK GP  ERNFG+F  DL+P+Y+IGILRPT  ++   + P++PV G   K+WC+ 
Sbjct: 317 LFNENLKSGPTSERNFGIFRSDLTPIYDIGILRPTFRSSDPVYNPRSPVRGSSSKRWCVT 376

Query: 374 KTGADSEALQRNIDYVCGMGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATDGNDYDC 433
           K GA++ ALQRNIDYVCG+GLDC PI E G C+ PNTV+AH+ Y MN Y+Q  + +++DC
Sbjct: 377 KAGAETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTVKAHSKYAMNLYYQTMEKHEFDC 436

Query: 434 DFDQTGALTTMDPS 445
           DFD TG +TT+DPS
Sbjct: 437 DFDNTGEITTIDPS 449

BLAST of MS006185 vs. TAIR 10
Match: AT2G16230.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 422.2 bits (1084), Expect = 5.4e-118
Identity = 210/424 (49.53%), Postives = 284/424 (66.98%), Query Frame = 0

Query: 28  IGVNYGTVADNLPPPSKVAQFLSDSTIINRVRLFDANPEILRAFAHTGISLTITVPNDQI 87
           IGVNYG ++DNLPPPS+ A+ L  ST I +VRL++A+  I+ +   TGI + I V N  +
Sbjct: 25  IGVNYGLLSDNLPPPSQTAKLL-QSTSIQKVRLYNADSSIITSLVGTGIGIVIGVANGDL 84

Query: 88  PRLAKP-NFAEDWIKFNVQPYVPATDIIRVLVGNEVLATANKLLIANLVPAMQNLHTALA 147
           P +A   N A  WI  NV P+ PA++II + VGNEVL + +  L+  L+PAMQN+  AL 
Sbjct: 85  PSIASDLNIASQWINSNVLPFYPASNIILINVGNEVLLSNDLNLVNQLLPAMQNVQKALE 144

Query: 148 GASLDRRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVLKPMLNFLRETNSAFMVNPYPF 207
             SL  +I+VST H++ +L NS PPS G F   Y    LK +L FL +T S F +NPYPF
Sbjct: 145 AVSLGGKIKVSTVHAMTVLGNSEPPSAGSFAPSYQAG-LKGILQFLSDTGSPFAINPYPF 204

Query: 208 FG----SSPETLDYALFRPNPGVSDPDSGLLYTNMLDAQLDAVFSAMKSLGFADVDIVIA 267
           F       PETL + LF+PNPG  D ++G+ Y NM DAQ+DAV SA+KS+GF  V++++A
Sbjct: 205 FAYQSDPRPETLAFCLFQPNPGRVDSNTGIKYMNMFDAQVDAVHSALKSIGFEKVEVLVA 264

Query: 268 ETGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPG 327
           ETGWPS GD  +VG   + A  +NGNL+ H+ S VGTPLMP ++ +TYIFALF+E+LKPG
Sbjct: 265 ETGWPSTGDSNEVGPSVENAKAYNGNLIAHLRSMVGTPLMPGKSIDTYIFALFDENLKPG 324

Query: 328 PICERNFGLFEPDLSPVYEIGILRPTVAATARTHPKTPVTGGKKWCLPKTGADSEALQRN 387
           P  E++FGLF+PDLS  Y+IG+ + T + T+++ P+        WC+PK  A  E LQ +
Sbjct: 325 PSFEQSFGLFKPDLSMAYDIGLTKTTSSQTSQS-PQLGKVTSMGWCVPKEDATQEQLQDS 384

Query: 388 IDYVCGMGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATDGNDYDCDFDQTGALTTMD 447
           +D+VCG G+DCGPI   G CF PN V +H AY MN YFQ +  N  DCDF +T  +T+ +
Sbjct: 385 LDWVCGQGIDCGPIMPGGVCFEPNNVASHTAYAMNLYFQKSPENPTDCDFSKTARITSEN 444

BLAST of MS006185 vs. TAIR 10
Match: AT3G55430.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 419.5 bits (1077), Expect = 3.5e-117
Identity = 211/421 (50.12%), Postives = 284/421 (67.46%), Query Frame = 0

Query: 27  SIGVNYGTVADNLPPPSKVAQFLSDSTIINRVRLFDANPEILRAFAHTGISLTITVPNDQ 86
           +IGVNYGT+  NLPPP++VA F+   T I+ V++FD NP+ILRAFA TGIS+ +TVPN  
Sbjct: 28  AIGVNYGTLG-NLPPPTQVANFIKTQTSIDSVKIFDVNPDILRAFAGTGISVVVTVPNGD 87

Query: 87  IPRLAKPNFAEDWIKFNVQPYVPATDIIRVLVGNEVLATANKLLIANLVPAMQNLHTALA 146
           IP LA    A  W+  N+ P+ P T I  + VGNE+L T +  +I NL+PAM+NL+ AL 
Sbjct: 88  IPALANGRQARRWVSVNILPFHPQTKIKYISVGNEILLTGDNNMINNLLPAMRNLNNALV 147

Query: 147 GASLDRRIQVSTPHSLGILSN--SSPPSTGRFRQGYDTHVLKPMLNFLRETNSAFMVNPY 206
            A + R ++V+T HSL I++   +  PS+GRFR G+D  +L P+L + R T S FMVNPY
Sbjct: 148 RAGV-RDVKVTTAHSLNIIAYDLTGAPSSGRFRPGWDKGILAPILAYHRRTKSPFMVNPY 207

Query: 207 PFFGSSPETLDYALFR-PNPGVSDPDSGLLYTNMLDAQLDAVFSAMKSLGFADVDIVIAE 266
           P+FG  P+ +++A+FR P   V DP +  +YTNM DA +D+ +SAMK+LG+ DV+IV+ E
Sbjct: 208 PYFGFDPKNVNFAIFRTPYKAVRDPFTRHVYTNMFDALMDSTYSAMKALGYGDVNIVVGE 267

Query: 267 TGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVGTPLMPNRTFETYIFALFNEDLKPGP 326
           TGWPS  D       P  AA FN N+++    G GTPLMPNR FETYIF LFNE+ KPGP
Sbjct: 268 TGWPSACDAP--WCSPANAAWFNLNIIKR-AQGQGTPLMPNRRFETYIFGLFNEEGKPGP 327

Query: 327 ICERNFGLFEPDLSPVYEIGILRPTVAATARTHPKTPVTGGKKWCLPKTGADSEALQRNI 386
             ERN+GLF  D SPVY++G+LR       R     P T G KWC+ ++GA +  LQ +I
Sbjct: 328 TAERNWGLFRADFSPVYDVGLLRNGQGGGGRPALPAPSTAGGKWCVARSGATNTQLQDSI 387

Query: 387 DYVCGMGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATDGNDYDCDFDQTGALTTMDP 445
           ++VCG G+DC PIQ  G CF P+++R HA++VMNAYFQ+    D  C+F  TG +   +P
Sbjct: 388 NWVCGQGVDCKPIQAGGSCFNPSSLRTHASFVMNAYFQSHGRTDGACNFSGTGMIVGNNP 443

BLAST of MS006185 vs. TAIR 10
Match: AT4G34480.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 419.5 bits (1077), Expect = 3.5e-117
Identity = 215/435 (49.43%), Postives = 285/435 (65.52%), Query Frame = 0

Query: 15  FLHYLCSGAKGGSIGVNYGTVADNLPPPSKVAQFLSDSTIINRVRLFDANPEILRAFAHT 74
           FL +  S      IGVNYG VADNLPPPS+  + L  ST I +VRL+ A+P I++A A T
Sbjct: 13  FLSHFPSSHAEPFIGVNYGQVADNLPPPSETVKLL-QSTSIQKVRLYGADPAIIKALAGT 72

Query: 75  GISLTITVPNDQIPRLAK-PNFAEDWIKFNVQPYVPATDIIRVLVGNEVLATANKLLIAN 134
           G+ + I   N  +P LA  PN A  WI  NV P+ PA+ I+ + VGNE+L + +  L+  
Sbjct: 73  GVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQ 132

Query: 135 LVPAMQNLHTALAGASLDRRIQVSTPHSLGILSNSSPPSTGRFRQGYDTHVLKPMLNFLR 194
           L+PAMQN+  AL   SL  +I+VST +S+ +L +S PPS+G F  GY T  LK +L FL 
Sbjct: 133 LLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTG-LKGILQFLS 192

Query: 195 ETNSAFMVNPYPFFG----SSPETLDYALFRPNPGVSDPDSGLLYTNMLDAQLDAVFSAM 254
           +T S F +NPYPFF       PETL + LF PN G  D  +G+ YTNM DAQ+DAV SA+
Sbjct: 193 DTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSAL 252

Query: 255 KSLGFADVDIVIAETGWPSKGDPAQVGIGPKEAADFNGNLMRHVVSGVGTPLMPNRTFET 314
           KS+GF  V+IV+AETGW S+GD  +VG     A  +NGNL+ H+ S VGTPLMP +  +T
Sbjct: 253 KSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDT 312

Query: 315 YIFALFNEDLKPGPICERNFGLFEPDLSPVYEIGILRPTVAATARTHPKTPVTGGKKWCL 374
           YIFAL++E+LKPGP  ER FGLF+ DLS VY++G+ +   ++++   P   VT    WC+
Sbjct: 313 YIFALYDENLKPGPSSERAFGLFKTDLSMVYDVGLAK---SSSSSQTPSGKVT-SSGWCV 372

Query: 375 PKTGADSEALQRNIDYVCGMGLDCGPIQENGPCFAPNTVRAHAAYVMNAYFQATDGNDYD 434
           PK GA +E LQ ++D+ CG G+DCG IQ  G CF PN V +HAAY MN YFQ +     D
Sbjct: 373 PKKGATNEELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAAYAMNMYFQKSPKQPTD 432

Query: 435 CDFDQTGALTTMDPS 445
           CDF +T  +T+ +PS
Sbjct: 433 CDFSKTATVTSQNPS 441