MS006116 (gene) Bitter gourd (TR) v1

Overview


Name	MS006116
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Aspergillus nuclease S(1)
Location	scaffold96: 169865 .. 172686 (-)
RNA-Seq Expression	MS006116
Synteny	MS006116

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCCTGAATCCAGACGCAACAGAGGCCGTCCAAAATTTACTACCGGAAAATGTCGACGGAAACCTGTCGGCGTTGTGTGTTTGGCCGGACCAAATCCGACATTGGTATCGGTATCGCTGGACCAGTCCTCTTCACTTCATTGACACGCCCGATAATTCCTGTTCTTTCCTCTACACGAGTAACCATTTTTTCTTCTCTCTTTCATCAAACAATCTTGTCCATTTTTTATTCTTTGGATTTTTAATTGGTGGCTGTTTATAGGAGATTGCCACGACAACGGCGGCGTCGAGAACATGTGCGTCGCCGGCGCCGTTCGCAATTTCACCACTCAGCTCGCGCACTACGCAGAAGGGGGCTCCGATCGTCGACGTAATTAACCCGACCCGGCTCCGGTCCAAACCCGATTTGTTTTATTTTACTTAAACCCGATTATTTGTTTTGAAAACGACGATTTTTCATGGTTACTGCAGATAACTTGACCGAGGCGTTATTATTTCTATCGCACTTCCTTGGGGATATTCATCAGGTAAATAAAATATTGATTAAAATATTAAATAATCTAATTTAGTCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGAGCTGAATTTTACGTTGTTCTCTGTAATTTAAAACCTCTTAATTTAGTATGGAGATCAAACTTTACAAATTATTTCCATTATACCATATTGAATACGAGTTAATGATTGGTATTTTATTTGAATTATTATATTAATTAATCGTGTCGGCTTAAAGATAAGTGAAATGTAAGTGTATTATCCAATAAATTTTTAGATATATCAGACCAAACTAAAATTTTTAAAATTATAGAGGCCGCTCTTAAAAGAAGTTGGAATAGCAAAAGAAATTTTATCCTCCGACTCCTCTATTTGATGAGATAGTCATTTTCCTTCTTAGTTTTTTTAATCTTTAACGAACAACTAATAATCAAAGGTAGTTTATGTAGTTTTTGAAAGAAAGAAGAGGTACATCGAAATTAATGAAACTAAAACTATAAGAAGTGAAAGGAGTAATTTAACCTAAAATGTCTATATCAATATGAAAAATTTTAATTAATTATCCGAGTGTAATTGTAAATTACAATTAACCCGGTGGTTTTACATCCAGCCAATGCACGTGGGGTTCACGAGCGACGAGGGAGGGAACACCATAAACTTACGGTGGTACCGCCACAAGTCCAATCTACACCACGTAAGCCCTAAGAAATAATTTAGACTTATAGCTTGAAATTAATTAATTATGACAATTCTTACTAATTAGGGAAAAAAAAAAAAACCACTGACGCAGGTGTGGGATAGGGAGATTATTCTCACAGCTCTAGCAGATTATTACGACAAGGACTCGGGCCTCCTCCTACAAGACATACAAAGGAATTTGACCCATGTAATTAATATAATTTATTTTACATTAATTAGGATAATATATATTAGTTTATAAGCTCATTTTTTAATACTAACTCAATTCTTGTAGGGAATTTGGACCCATGAGATTCCAACATGGCAGCATTGCGAAGATCTTGATTCATGCGTAAACAAGTATATGTTTATTTATATATTTGATCTCTCTATTTTATTTTTTTTCTCTCTAAATAATGCTTCATTTTTTCCTTTTTTTAATGGAGGTGGGCTGAGGAGAGTATAAGCTTAGCTTGCAAGTGGGGTTATGAAGGAGTTGAGGCTGGTACAACTTTAGCAGGTAAATCTAATCAAACTTAAATTAATGGTCTTTCTTTAGTATAATAATAGGAAAGTAGATTTAAATTCACGATTTCTTGGTTCGGAATATATGTCTGTTCAAATAGGCTAAATTAAGTTCCTTAATCATAACATAAATAATGAAGAATCAATAATCAAATTAAGTCCATTATTGGTTCTCTATTTATTTTAATAATGGATTAGTTATACTAAGTCTTCAATAATGAAGAATCAATATTCAAATTAGGCCCATTACCTGTCCTCTATTCATCTTAATAATGGGTTGGTTATACTGAAGTATTTTTTATATTTAGGGGCCTGCTTTAGATTATTTTGTTTTTAAAAAAATTGGATTTGTTTGTTAACATTTTATTTGTCTCATAGTCCATATTTAGAATAATTGTGAAAAATTATAAGAAATACTATAATGTTTTATGTTCACTTTTCAATTTGAAAAAAAGAAATTAAGTTTATTACATTATTCAGTTTATCGCTTTGTATTTCATTTTTCGGGAACATTTTTGAAGTTTAATCAAAATATTAAAACATAAACACATTTTAAAAACTATCTTGTCAAGTTTTAAAAAATCTAGATATGGCCAATTTGAAAACATTAAGAAAAGGAACAAAATGAATATAGTAAAGTATACATTTCCAATAAAAAACAACAGTATCTATCAGGAAAAATATGGAGCAGTTTTTCTAAAATAATTTGGGTAGCTAATTAAGAGATAATATTCTCTAAAATTTTTTTAAAAAAAAGCTAATTAAGAGATGATAAATCTCTAACCAATGTGATTTTTGGGCAGAGGATTACTTCGATTCAAGGATGCCAATTGTGATGAAACGCATAGCTGAAGGTGGAGTCCGGTTGGCCATGCTTTTGAACCGGGTTTTTTCTGATGGAGGAGGAGGATTCGCCTCCTCAACT

mRNA sequence

CTCCTGAATCCAGACGCAACAGAGGCCGTCCAAAATTTACTACCGGAAAATGTCGACGGAAACCTGTCGGCGTTGTGTGTTTGGCCGGACCAAATCCGACATTGGTATCGGTATCGCTGGACCAGTCCTCTTCACTTCATTGACACGCCCGATAATTCCTGTTCTTTCCTCTACACGAGAGATTGCCACGACAACGGCGGCGTCGAGAACATGTGCGTCGCCGGCGCCGTTCGCAATTTCACCACTCAGCTCGCGCACTACGCAGAAGGGGGCTCCGATCGTCGACATAACTTGACCGAGGCGTTATTATTTCTATCGCACTTCCTTGGGGATATTCATCAGCCAATGCACGTGGGGTTCACGAGCGACGAGGGAGGGAACACCATAAACTTACGGTGGTACCGCCACAAGTCCAATCTACACCACGTGTGGGATAGGGAGATTATTCTCACAGCTCTAGCAGATTATTACGACAAGGACTCGGGCCTCCTCCTACAAGACATACAAAGGAATTTGACCCATGGAATTTGGACCCATGAGATTCCAACATGGCAGCATTGCGAAGATCTTGATTCATGCGTAAACAAGTGGGCTGAGGAGAGTATAAGCTTAGCTTGCAAGTGGGGTTATGAAGGAGTTGAGGCTGGTACAACTTTAGCAGAGGATTACTTCGATTCAAGGATGCCAATTGTGATGAAACGCATAGCTGAAGGTGGAGTCCGGTTGGCCATGCTTTTGAACCGGGTTTTTTCTGATGGAGGAGGAGGATTCGCCTCCTCAACT

Coding sequence (CDS)

CTCCTGAATCCAGACGCAACAGAGGCCGTCCAAAATTTACTACCGGAAAATGTCGACGGAAACCTGTCGGCGTTGTGTGTTTGGCCGGACCAAATCCGACATTGGTATCGGTATCGCTGGACCAGTCCTCTTCACTTCATTGACACGCCCGATAATTCCTGTTCTTTCCTCTACACGAGAGATTGCCACGACAACGGCGGCGTCGAGAACATGTGCGTCGCCGGCGCCGTTCGCAATTTCACCACTCAGCTCGCGCACTACGCAGAAGGGGGCTCCGATCGTCGACATAACTTGACCGAGGCGTTATTATTTCTATCGCACTTCCTTGGGGATATTCATCAGCCAATGCACGTGGGGTTCACGAGCGACGAGGGAGGGAACACCATAAACTTACGGTGGTACCGCCACAAGTCCAATCTACACCACGTGTGGGATAGGGAGATTATTCTCACAGCTCTAGCAGATTATTACGACAAGGACTCGGGCCTCCTCCTACAAGACATACAAAGGAATTTGACCCATGGAATTTGGACCCATGAGATTCCAACATGGCAGCATTGCGAAGATCTTGATTCATGCGTAAACAAGTGGGCTGAGGAGAGTATAAGCTTAGCTTGCAAGTGGGGTTATGAAGGAGTTGAGGCTGGTACAACTTTAGCAGAGGATTACTTCGATTCAAGGATGCCAATTGTGATGAAACGCATAGCTGAAGGTGGAGTCCGGTTGGCCATGCTTTTGAACCGGGTTTTTTCTGATGGAGGAGGAGGATTCGCCTCCTCAACT

Protein sequence

LLNPDATEAVQNLLPENVDGNLSALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHDNGGVENMCVAGAVRNFTTQLAHYAEGGSDRRHNLTEALLFLSHFLGDIHQPMHVGFTSDEGGNTINLRWYRHKSNLHHVWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPTWQHCEDLDSCVNKWAEESISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRLAMLLNRVFSDGGGGFASST

Homology

[Close]

BLAST of MS006116 vs. NCBI nr
Match: XP_022140160.1 (endonuclease 1 [Momordica charantia])

HSP 1 Score: 565.1 bits (1455), Expect = 3.2e-157
Identity = 260/261 (99.62%), Postives = 260/261 (99.62%), Query Frame = 0


Query: 1   LLNPDATEAVQNLLPENVDGNLSALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTR 60
           LLNPDATEAVQNLLPENVDGNLSALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTR
Sbjct: 113 LLNPDATEAVQNLLPENVDGNLSALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTR 172

Query: 61  DCHDNGGVENMCVAGAVRNFTTQLAHYAEGGSDRRHNLTEALLFLSHFLGDIHQPMHVGF 120
           DCHDNGGVENMCVAGAVRNFTTQLAHYAEGGSDRRHNLT ALLFLSHFLGDIHQPMHVGF
Sbjct: 173 DCHDNGGVENMCVAGAVRNFTTQLAHYAEGGSDRRHNLTGALLFLSHFLGDIHQPMHVGF 232

Query: 121 TSDEGGNTINLRWYRHKSNLHHVWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHE 180
           TSDEGGNTINLRWYRHKSNLHHVWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHE
Sbjct: 233 TSDEGGNTINLRWYRHKSNLHHVWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHE 292

Query: 181 IPTWQHCEDLDSCVNKWAEESISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGV 240
           IPTWQHCEDLDSCVNKWAEESISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGV
Sbjct: 293 IPTWQHCEDLDSCVNKWAEESISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGV 352

Query: 241 RLAMLLNRVFSDGGGGFASST 262
           RLAMLLNRVFSDGGGGFASST
Sbjct: 353 RLAMLLNRVFSDGGGGFASST 373

[Close]

BLAST of MS006116 vs. NCBI nr
Match: XP_020221420.1 (endonuclease 1 [Cajanus cajan] >KYP63013.1 Nuclease PA3 [Cajanus cajan])

HSP 1 Score: 464.5 bits (1194), Expect = 5.9e-127
Identity = 202/261 (77.39%), Postives = 236/261 (90.42%), Query Frame = 0


Query: 1   LLNPDATEAVQNLLPENVDGNLSALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTR 60
           LL P+A EAV +LLPE V+GNLSALC+WPDQIRHWY+YRWTSPLHFIDTPDN+CSF Y+R
Sbjct: 48  LLEPEAAEAVHHLLPEYVNGNLSALCIWPDQIRHWYKYRWTSPLHFIDTPDNACSFKYSR 107

Query: 61  DCHDNGGVENMCVAGAVRNFTTQLAHYAEGGSDRRHNLTEALLFLSHFLGDIHQPMHVGF 120
           DCHD  GVE+MCVAGAV+NFT+QL HY+EG SDRRHN+TEALLFLSHF+GDIHQPMHVGF
Sbjct: 108 DCHDPHGVEDMCVAGAVKNFTSQLMHYSEGTSDRRHNMTEALLFLSHFMGDIHQPMHVGF 167

Query: 121 TSDEGGNTINLRWYRHKSNLHHVWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHE 180
           T+DEGGNTINLRW+RHKSNLHHVWDREIILTALADYYDKD  LLLQDI+RN T GIW+ +
Sbjct: 168 TTDEGGNTINLRWFRHKSNLHHVWDREIILTALADYYDKDVSLLLQDIERNYTDGIWSDD 227

Query: 181 IPTWQHCEDLDSCVNKWAEESISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGV 240
           + +W++C D+  CVN WA+ESI +ACKWGYEGVE+GTTLA+DYFDSRMP VMKRIA+GG+
Sbjct: 228 VTSWKNCNDISQCVNNWAKESIQIACKWGYEGVESGTTLADDYFDSRMPYVMKRIAQGGI 287

Query: 241 RLAMLLNRVFSDGGGGFASST 262
           RLAM+LN+VF+D   G A++T
Sbjct: 288 RLAMILNKVFADSEEGLATAT 308

[Close]

BLAST of MS006116 vs. NCBI nr
Match: TKY71638.1 (Endonuclease 1 [Spatholobus suberectus])

HSP 1 Score: 458.4 bits (1178), Expect = 4.2e-125
Identity = 200/261 (76.63%), Postives = 235/261 (90.04%), Query Frame = 0


Query: 1   LLNPDATEAVQNLLPENVDGNLSALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTR 60
           LL P+A+EAV +LLP+ V GNLSALCVWPDQIRHW++YRWTSPLHFIDTPD++CSF Y+R
Sbjct: 50  LLEPEASEAVYHLLPDYVKGNLSALCVWPDQIRHWHKYRWTSPLHFIDTPDDACSFQYSR 109

Query: 61  DCHDNGGVENMCVAGAVRNFTTQLAHYAEGGSDRRHNLTEALLFLSHFLGDIHQPMHVGF 120
           DCHD+ GVE+MCVAGAV+NFT+QL HY EG SDRR+N+TEALLFLSHF+GDIHQPMHVGF
Sbjct: 110 DCHDSHGVEDMCVAGAVKNFTSQLMHYREGTSDRRYNMTEALLFLSHFMGDIHQPMHVGF 169

Query: 121 TSDEGGNTINLRWYRHKSNLHHVWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHE 180
           T+DEGGNTI LRWYRHKSNLHHVWDREIILTALADYYDKD  LLLQDI+RN T GIW+ +
Sbjct: 170 TTDEGGNTIELRWYRHKSNLHHVWDREIILTALADYYDKDVSLLLQDIERNYTDGIWSDD 229

Query: 181 IPTWQHCEDLDSCVNKWAEESISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGV 240
           + +W+HC D+  CVN WA+ESI +ACKWGYEGVE+ TTLA+DYFDSRMP VMKRIA+GG+
Sbjct: 230 VSSWKHCNDISRCVNNWAKESIQIACKWGYEGVESRTTLADDYFDSRMPFVMKRIAQGGI 289

Query: 241 RLAMLLNRVFSDGGGGFASST 262
           RLAM+LN+VF+D   GFA++T
Sbjct: 290 RLAMILNKVFADSEEGFATAT 310

[Close]

BLAST of MS006116 vs. NCBI nr
Match: NP_001235325.1 (endonuclease [Glycine max] >ABP68856.1 endonuclease [Glycine max])

HSP 1 Score: 458.0 bits (1177), Expect = 5.5e-125
Identity = 198/261 (75.86%), Postives = 232/261 (88.89%), Query Frame = 0


Query: 1   LLNPDATEAVQNLLPENVDGNLSALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTR 60
           LL P+A+EAV  LLP++V GNLSALC WPDQIRHWY+YRWTSPLHFIDTPDN+CSF Y+R
Sbjct: 48  LLEPEASEAVYQLLPDHVKGNLSALCTWPDQIRHWYKYRWTSPLHFIDTPDNACSFQYSR 107

Query: 61  DCHDNGGVENMCVAGAVRNFTTQLAHYAEGGSDRRHNLTEALLFLSHFLGDIHQPMHVGF 120
           DCHD  GVE+MCVAGAV+NFT+QL HY EG SDRR+N+TEALLFLSHF+GDIHQPMHVGF
Sbjct: 108 DCHDPQGVEDMCVAGAVKNFTSQLMHYKEGTSDRRYNMTEALLFLSHFMGDIHQPMHVGF 167

Query: 121 TSDEGGNTINLRWYRHKSNLHHVWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHE 180
           T+DEGGNTI LRW+RHKSNLHHVWDREIILT LADYYDKD   LLQDI+RN T GIW+ +
Sbjct: 168 TTDEGGNTIELRWFRHKSNLHHVWDREIILTGLADYYDKDVSFLLQDIERNYTDGIWSDD 227

Query: 181 IPTWQHCEDLDSCVNKWAEESISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGV 240
           + +W+HC D+  CVN WA+ESI +ACKWGYEGV+ GTTLA+DYFDSRMP+VMKRIA+GG+
Sbjct: 228 VTSWKHCNDISQCVNNWAKESIQIACKWGYEGVQPGTTLADDYFDSRMPLVMKRIAQGGI 287

Query: 241 RLAMLLNRVFSDGGGGFASST 262
           RLAM+LN+VF+D   GFA++T
Sbjct: 288 RLAMILNKVFADSEEGFATAT 308

[Close]

BLAST of MS006116 vs. NCBI nr
Match: XP_028203080.1 (endonuclease 1-like [Glycine soja] >KAG4948408.1 hypothetical protein JHK86_041647 [Glycine max] >KAG4945541.1 hypothetical protein JHK87_041548 [Glycine soja] >KAG5104622.1 hypothetical protein JHK82_041592 [Glycine max] >KAG5115746.1 hypothetical protein JHK84_041859 [Glycine max] >KRH10768.1 hypothetical protein GLYMA_15G068600v4 [Glycine max])

HSP 1 Score: 457.2 bits (1175), Expect = 9.4e-125
Identity = 198/261 (75.86%), Postives = 231/261 (88.51%), Query Frame = 0


Query: 1   LLNPDATEAVQNLLPENVDGNLSALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTR 60
           LL P+A+EAV  LLP++V GNLSALC WPDQIRHWY+YRWTSPLHFIDTPDN+CSF Y+R
Sbjct: 48  LLEPEASEAVYQLLPDHVKGNLSALCTWPDQIRHWYKYRWTSPLHFIDTPDNACSFQYSR 107

Query: 61  DCHDNGGVENMCVAGAVRNFTTQLAHYAEGGSDRRHNLTEALLFLSHFLGDIHQPMHVGF 120
           DCHD  GVE+MCVAGAV+NFT+QL HY EG SDRR+N+TEALLFLSHF+GDIHQPMHVGF
Sbjct: 108 DCHDPQGVEDMCVAGAVKNFTSQLMHYKEGTSDRRYNMTEALLFLSHFMGDIHQPMHVGF 167

Query: 121 TSDEGGNTINLRWYRHKSNLHHVWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHE 180
           T+DEGGNTI LRW+RHKSNLHHVWDREIILT LADYYDKD   LLQDI+RN T GIW+ +
Sbjct: 168 TTDEGGNTIELRWFRHKSNLHHVWDREIILTGLADYYDKDVSFLLQDIERNYTDGIWSDD 227

Query: 181 IPTWQHCEDLDSCVNKWAEESISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGV 240
           + +W+HC D+  CVN WA+ESI +ACKWGYEGV+ GTTLA+DYFDSRMP VMKRIA+GG+
Sbjct: 228 VTSWKHCNDISQCVNNWAKESIQIACKWGYEGVQPGTTLADDYFDSRMPFVMKRIAQGGI 287

Query: 241 RLAMLLNRVFSDGGGGFASST 262
           RLAM+LN+VF+D   GFA++T
Sbjct: 288 RLAMILNKVFADSEEGFATAT 308

[Close]

BLAST of MS006116 vs. ExPASy Swiss-Prot
Match: Q9SXA6 (Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 9.5e-112
Identity = 173/252 (68.65%), Postives = 217/252 (86.11%), Query Frame = 0


Query: 1   LLNPDATEAVQNLLPENVDGNLSALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTR 60
           LL       V+NLLP+ V G+LSALCVWPDQIRHWY+YRWTS LH+IDTPD +CS+ Y+R
Sbjct: 44  LLEAGPAHVVENLLPDYVKGDLSALCVWPDQIRHWYKYRWTSHLHYIDTPDQACSYEYSR 103

Query: 61  DCHDNGGVENMCVAGAVRNFTTQLAHYAEGGSDRRHNLTEALLFLSHFLGDIHQPMHVGF 120
           DCHD  G+++MCV GA++NFT+QL HY EG SDRR+N+TEALLFLSHF+GDIHQPMHVGF
Sbjct: 104 DCHDQHGLKDMCVDGAIQNFTSQLQHYGEGTSDRRYNMTEALLFLSHFMGDIHQPMHVGF 163

Query: 121 TSDEGGNTINLRWYRHKSNLHHVWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHE 180
           TSDEGGNTI+LRWY+HKSNLHHVWDREIILTAL + YDK+  LL +D+++N+T+G+W  +
Sbjct: 164 TSDEGGNTIDLRWYKHKSNLHHVWDREIILTALKENYDKNLDLLQEDLEKNITNGLWHDD 223

Query: 181 IPTWQHCEDLDSCVNKWAEESISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGV 240
           + +W  C DL +C +K+A ESI LACKWGY+GV++G TL+E+YF++R+PIVMKRI +GGV
Sbjct: 224 LSSWTECNDLIACPHKYASESIKLACKWGYKGVKSGETLSEEYFNTRLPIVMKRIVQGGV 283

Query: 241 RLAMLLNRVFSD 253
           RLAM+LNRVFSD
Sbjct: 284 RLAMILNRVFSD 295

[Close]

BLAST of MS006116 vs. ExPASy Swiss-Prot
Match: F4JJL0 (Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=1 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 2.7e-82
Identity = 134/249 (53.82%), Postives = 185/249 (74.30%), Query Frame = 0


Query: 5   DATEAVQNLLPENVDGNLSALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHD 64
           +   AV+ LLP++ DG+L+++C WPD+I+H +++RWTSPLH++DTPD  C++ Y RDCHD
Sbjct: 44  ETVAAVKKLLPKSADGDLASVCSWPDEIKHHWQWRWTSPLHYVDTPDYRCNYEYCRDCHD 103

Query: 65  NGGVENMCVAGAVRNFTTQLAHYAEGGSDRRH-NLTEALLFLSHFLGDIHQPMHVGFTSD 124
               ++ CV GA+ N+T QL   +E      H NLTEAL+FLSHF+GDIHQP+HVGF  D
Sbjct: 104 THKNQDRCVTGAIFNYTMQLMSASENSDTIVHYNLTEALMFLSHFIGDIHQPLHVGFLGD 163

Query: 125 EGGNTINLRWYRHKSNLHHVWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPT 184
           EGGNTI +RWYR K+NLHHVWD  II +AL  YY+K   L+++ +Q NLT+  W++++P 
Sbjct: 164 EGGNTITVRWYRRKTNLHHVWDNMIIESALKTYYNKSLPLMIEALQANLTND-WSNDVPL 223

Query: 185 WQHCE-DLDSCVNKWAEESISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRL 244
           W+ C+ +  +C N +A ESI+LACK+ Y     GTTL +DYF SR+PIV KR+A+GG+RL
Sbjct: 224 WESCQLNQTACPNPYASESINLACKYAYRNATPGTTLGDDYFLSRLPIVEKRLAQGGIRL 283

Query: 245 AMLLNRVFS 252
           A  LNR+FS
Sbjct: 284 AATLNRIFS 291

[Close]

BLAST of MS006116 vs. ExPASy Swiss-Prot
Match: Q9C9G4 (Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 2.1e-74
Identity = 125/250 (50.00%), Postives = 177/250 (70.80%), Query Frame = 0


Query: 2   LNPDATEAVQNLLPENVDGNLSALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRD 61
           L+  A +AV+ LLPE+ +G+LS+LC+W D+++  +RY W+SPLH+I+TPD +CS+ Y RD
Sbjct: 44  LDETAAKAVKELLPESAEGDLSSLCLWADRVK--FRYHWSSPLHYINTPD-ACSYQYNRD 103

Query: 62  CHDNGGVENMCVAGAVRNFTTQLAHYAEGGSDR-RHNLTEALLFLSHFLGDIHQPMHVGF 121
           C D  G +  CVAGA+ N+TTQL  Y    S + ++NLTEALLF+SHF+GDIHQP+HV +
Sbjct: 104 CKDESGEKGRCVAGAIYNYTTQLLSYKTAASSQSQYNLTEALLFVSHFMGDIHQPLHVSY 163

Query: 122 TSDEGGNTINLRWYRHKSNLHHVWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHE 181
            SD+GGNTI + WY  K+NLHH+WD  II TA AD Y+     ++  +++N+T   W  +
Sbjct: 164 ASDKGGNTIEVHWYTRKANLHHIWDSNIIETAEADLYNSALEGMVDALKKNITTE-WADQ 223

Query: 182 IPTWQHCEDLDSCVNKWAEESISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGV 241
           +  W+ C    +C + +A E I  AC W Y+GV  G TL ++YF SR+PIV +R+A+GGV
Sbjct: 224 VKRWETCTKKTACPDIYASEGIQAACDWAYKGVTEGDTLEDEYFYSRLPIVYQRLAQGGV 283

Query: 242 RLAMLLNRVF 251
           RLA  LNR+F
Sbjct: 284 RLAATLNRIF 289

[Close]

BLAST of MS006116 vs. ExPASy Swiss-Prot
Match: F4JJL3 (Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=1 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 2.5e-72
Identity = 124/250 (49.60%), Postives = 173/250 (69.20%), Query Frame = 0


Query: 5   DATEAVQNLLPENVD-GNLSALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCH 64
           D   AV+ LLPE+VD G L+  C WPD+I+   +++WTS LH+++TP+  C++ Y RDCH
Sbjct: 40  DTIAAVKKLLPESVDGGGLADFCSWPDEIKKLSQWQWTSTLHYVNTPEYRCNYEYCRDCH 99

Query: 65  DNGGVENMCVAGAVRNFTTQLAHYAEGGSDRRH-NLTEALLFLSHFLGDIHQPMHVGFTS 124
           D    ++ CV GA+ N+T QL   +E   +  H NLTEALLFLSH++GD+HQP+H GF  
Sbjct: 100 DTHKHKDWCVTGAIFNYTNQLMSASENSQNIVHYNLTEALLFLSHYMGDVHQPLHTGFLG 159

Query: 125 DEGGNTINLRWYRHKSNLHHVWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIP 184
           D GGNTI + WY +KSNLHHVWD  II +AL  YY+     ++Q +Q  L +G W++++P
Sbjct: 160 DLGGNTIIVNWYHNKSNLHHVWDNMIIDSALETYYNSSLPHMIQALQAKLKNG-WSNDVP 219

Query: 185 TWQHCE-DLDSCVNKWAEESISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVR 244
           +W+ C     +C N +A ESI LACK+ Y     GTTL ++YF SR+P+V KR+A+GG+R
Sbjct: 220 SWKSCHFHQKACPNLYASESIDLACKYAYRNATPGTTLGDEYFLSRLPVVEKRLAQGGIR 279

Query: 245 LAMLLNRVFS 252
           LA  LNR+FS
Sbjct: 280 LAATLNRIFS 288

[Close]

BLAST of MS006116 vs. ExPASy Swiss-Prot
Match: Q8LDW6 (Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=1 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 4.0e-70
Identity = 121/249 (48.59%), Postives = 170/249 (68.27%), Query Frame = 0


Query: 5   DATEAVQNLLPENVDGNLSALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHD 64
           D   AV+ LLPE+ +G L+A+C WPD+I+   ++RWTS LHF DTPD  C++ Y+RDC  
Sbjct: 44  DTVVAVKKLLPESANGELAAVCSWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSRDC-- 103

Query: 65  NGGVENMCVAGAVRNFTTQLAHYAEGGSDRRH-NLTEALLFLSHFLGDIHQPMHVGFTSD 124
               ++ CV GA+ N+T QL   +E      H NLTEAL+FLSH++GDIHQP+H GF  D
Sbjct: 104 ---PKDWCVTGAIFNYTNQLMSTSENSQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGD 163

Query: 125 EGGNTINLRWYRHKSNLHHVWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPT 184
            GGN I + WY  ++NLH VWD  II +AL  YY+     ++ ++Q  L +G W++++P+
Sbjct: 164 LGGNKIKVHWYNQETNLHRVWDDMIIESALETYYNSSLPRMIHELQAKLKNG-WSNDVPS 223

Query: 185 WQHCE-DLDSCVNKWAEESISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRL 244
           W+ C+ +  +C N +A ESI LACK+ Y    AGTTL + YF SR+P+V KR+A+GG+RL
Sbjct: 224 WESCQLNQTACPNPYASESIDLACKYAYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRL 283

Query: 245 AMLLNRVFS 252
           A  LNR+FS
Sbjct: 284 AGTLNRIFS 286

[Close]

BLAST of MS006116 vs. ExPASy TrEMBL
Match: A0A6J1CEC2 (Aspergillus nuclease S(1) OS=Momordica charantia OX=3673 GN=LOC111010892 PE=3 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 1.5e-157
Identity = 260/261 (99.62%), Postives = 260/261 (99.62%), Query Frame = 0


Query: 1   LLNPDATEAVQNLLPENVDGNLSALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTR 60
           LLNPDATEAVQNLLPENVDGNLSALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTR
Sbjct: 113 LLNPDATEAVQNLLPENVDGNLSALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTR 172

Query: 61  DCHDNGGVENMCVAGAVRNFTTQLAHYAEGGSDRRHNLTEALLFLSHFLGDIHQPMHVGF 120
           DCHDNGGVENMCVAGAVRNFTTQLAHYAEGGSDRRHNLT ALLFLSHFLGDIHQPMHVGF
Sbjct: 173 DCHDNGGVENMCVAGAVRNFTTQLAHYAEGGSDRRHNLTGALLFLSHFLGDIHQPMHVGF 232

Query: 121 TSDEGGNTINLRWYRHKSNLHHVWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHE 180
           TSDEGGNTINLRWYRHKSNLHHVWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHE
Sbjct: 233 TSDEGGNTINLRWYRHKSNLHHVWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHE 292

Query: 181 IPTWQHCEDLDSCVNKWAEESISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGV 240
           IPTWQHCEDLDSCVNKWAEESISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGV
Sbjct: 293 IPTWQHCEDLDSCVNKWAEESISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGV 352

Query: 241 RLAMLLNRVFSDGGGGFASST 262
           RLAMLLNRVFSDGGGGFASST
Sbjct: 353 RLAMLLNRVFSDGGGGFASST 373

[Close]

BLAST of MS006116 vs. ExPASy TrEMBL
Match: A0A151T7J6 (Aspergillus nuclease S(1) OS=Cajanus cajan OX=3821 GN=KK1_017575 PE=3 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 2.8e-127
Identity = 202/261 (77.39%), Postives = 236/261 (90.42%), Query Frame = 0


Query: 1   LLNPDATEAVQNLLPENVDGNLSALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTR 60
           LL P+A EAV +LLPE V+GNLSALC+WPDQIRHWY+YRWTSPLHFIDTPDN+CSF Y+R
Sbjct: 48  LLEPEAAEAVHHLLPEYVNGNLSALCIWPDQIRHWYKYRWTSPLHFIDTPDNACSFKYSR 107

Query: 61  DCHDNGGVENMCVAGAVRNFTTQLAHYAEGGSDRRHNLTEALLFLSHFLGDIHQPMHVGF 120
           DCHD  GVE+MCVAGAV+NFT+QL HY+EG SDRRHN+TEALLFLSHF+GDIHQPMHVGF
Sbjct: 108 DCHDPHGVEDMCVAGAVKNFTSQLMHYSEGTSDRRHNMTEALLFLSHFMGDIHQPMHVGF 167

Query: 121 TSDEGGNTINLRWYRHKSNLHHVWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHE 180
           T+DEGGNTINLRW+RHKSNLHHVWDREIILTALADYYDKD  LLLQDI+RN T GIW+ +
Sbjct: 168 TTDEGGNTINLRWFRHKSNLHHVWDREIILTALADYYDKDVSLLLQDIERNYTDGIWSDD 227

Query: 181 IPTWQHCEDLDSCVNKWAEESISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGV 240
           + +W++C D+  CVN WA+ESI +ACKWGYEGVE+GTTLA+DYFDSRMP VMKRIA+GG+
Sbjct: 228 VTSWKNCNDISQCVNNWAKESIQIACKWGYEGVESGTTLADDYFDSRMPYVMKRIAQGGI 287

Query: 241 RLAMLLNRVFSDGGGGFASST 262
           RLAM+LN+VF+D   G A++T
Sbjct: 288 RLAMILNKVFADSEEGLATAT 308

[Close]

BLAST of MS006116 vs. ExPASy TrEMBL
Match: A5A338 (Aspergillus nuclease S(1) OS=Glycine max OX=3847 GN=EN1 PE=2 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 2.7e-125
Identity = 198/261 (75.86%), Postives = 232/261 (88.89%), Query Frame = 0


Query: 1   LLNPDATEAVQNLLPENVDGNLSALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTR 60
           LL P+A+EAV  LLP++V GNLSALC WPDQIRHWY+YRWTSPLHFIDTPDN+CSF Y+R
Sbjct: 48  LLEPEASEAVYQLLPDHVKGNLSALCTWPDQIRHWYKYRWTSPLHFIDTPDNACSFQYSR 107

Query: 61  DCHDNGGVENMCVAGAVRNFTTQLAHYAEGGSDRRHNLTEALLFLSHFLGDIHQPMHVGF 120
           DCHD  GVE+MCVAGAV+NFT+QL HY EG SDRR+N+TEALLFLSHF+GDIHQPMHVGF
Sbjct: 108 DCHDPQGVEDMCVAGAVKNFTSQLMHYKEGTSDRRYNMTEALLFLSHFMGDIHQPMHVGF 167

Query: 121 TSDEGGNTINLRWYRHKSNLHHVWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHE 180
           T+DEGGNTI LRW+RHKSNLHHVWDREIILT LADYYDKD   LLQDI+RN T GIW+ +
Sbjct: 168 TTDEGGNTIELRWFRHKSNLHHVWDREIILTGLADYYDKDVSFLLQDIERNYTDGIWSDD 227

Query: 181 IPTWQHCEDLDSCVNKWAEESISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGV 240
           + +W+HC D+  CVN WA+ESI +ACKWGYEGV+ GTTLA+DYFDSRMP+VMKRIA+GG+
Sbjct: 228 VTSWKHCNDISQCVNNWAKESIQIACKWGYEGVQPGTTLADDYFDSRMPLVMKRIAQGGI 287

Query: 241 RLAMLLNRVFSDGGGGFASST 262
           RLAM+LN+VF+D   GFA++T
Sbjct: 288 RLAMILNKVFADSEEGFATAT 308

[Close]

BLAST of MS006116 vs. ExPASy TrEMBL
Match: I1MEB5 (Aspergillus nuclease S(1) OS=Glycine max OX=3847 GN=GLYMA_15G068600 PE=3 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 4.5e-125
Identity = 198/261 (75.86%), Postives = 231/261 (88.51%), Query Frame = 0


Query: 1   LLNPDATEAVQNLLPENVDGNLSALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTR 60
           LL P+A+EAV  LLP++V GNLSALC WPDQIRHWY+YRWTSPLHFIDTPDN+CSF Y+R
Sbjct: 48  LLEPEASEAVYQLLPDHVKGNLSALCTWPDQIRHWYKYRWTSPLHFIDTPDNACSFQYSR 107

Query: 61  DCHDNGGVENMCVAGAVRNFTTQLAHYAEGGSDRRHNLTEALLFLSHFLGDIHQPMHVGF 120
           DCHD  GVE+MCVAGAV+NFT+QL HY EG SDRR+N+TEALLFLSHF+GDIHQPMHVGF
Sbjct: 108 DCHDPQGVEDMCVAGAVKNFTSQLMHYKEGTSDRRYNMTEALLFLSHFMGDIHQPMHVGF 167

Query: 121 TSDEGGNTINLRWYRHKSNLHHVWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHE 180
           T+DEGGNTI LRW+RHKSNLHHVWDREIILT LADYYDKD   LLQDI+RN T GIW+ +
Sbjct: 168 TTDEGGNTIELRWFRHKSNLHHVWDREIILTGLADYYDKDVSFLLQDIERNYTDGIWSDD 227

Query: 181 IPTWQHCEDLDSCVNKWAEESISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGV 240
           + +W+HC D+  CVN WA+ESI +ACKWGYEGV+ GTTLA+DYFDSRMP VMKRIA+GG+
Sbjct: 228 VTSWKHCNDISQCVNNWAKESIQIACKWGYEGVQPGTTLADDYFDSRMPFVMKRIAQGGI 287

Query: 241 RLAMLLNRVFSDGGGGFASST 262
           RLAM+LN+VF+D   GFA++T
Sbjct: 288 RLAMILNKVFADSEEGFATAT 308

[Close]

BLAST of MS006116 vs. ExPASy TrEMBL
Match: A0A445GQE8 (Aspergillus nuclease S(1) OS=Glycine soja OX=3848 GN=D0Y65_040182 PE=3 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 4.5e-125
Identity = 198/261 (75.86%), Postives = 231/261 (88.51%), Query Frame = 0


Query: 1   LLNPDATEAVQNLLPENVDGNLSALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTR 60
           LL P+A+EAV  LLP++V GNLSALC WPDQIRHWY+YRWTSPLHFIDTPDN+CSF Y+R
Sbjct: 48  LLEPEASEAVYQLLPDHVKGNLSALCTWPDQIRHWYKYRWTSPLHFIDTPDNACSFQYSR 107

Query: 61  DCHDNGGVENMCVAGAVRNFTTQLAHYAEGGSDRRHNLTEALLFLSHFLGDIHQPMHVGF 120
           DCHD  GVE+MCVAGAV+NFT+QL HY EG SDRR+N+TEALLFLSHF+GDIHQPMHVGF
Sbjct: 108 DCHDPQGVEDMCVAGAVKNFTSQLMHYKEGTSDRRYNMTEALLFLSHFMGDIHQPMHVGF 167

Query: 121 TSDEGGNTINLRWYRHKSNLHHVWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHE 180
           T+DEGGNTI LRW+RHKSNLHHVWDREIILT LADYYDKD   LLQDI+RN T GIW+ +
Sbjct: 168 TTDEGGNTIELRWFRHKSNLHHVWDREIILTGLADYYDKDVSFLLQDIERNYTDGIWSDD 227

Query: 181 IPTWQHCEDLDSCVNKWAEESISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGV 240
           + +W+HC D+  CVN WA+ESI +ACKWGYEGV+ GTTLA+DYFDSRMP VMKRIA+GG+
Sbjct: 228 VTSWKHCNDISQCVNNWAKESIQIACKWGYEGVQPGTTLADDYFDSRMPFVMKRIAQGGI 287

Query: 241 RLAMLLNRVFSDGGGGFASST 262
           RLAM+LN+VF+D   GFA++T
Sbjct: 288 RLAMILNKVFADSEEGFATAT 308

[Close]

BLAST of MS006116 vs. TAIR 10
Match: AT1G11190.1 (bifunctional nuclease i )

HSP 1 Score: 404.4 bits (1038), Expect = 6.7e-113
Identity = 173/252 (68.65%), Postives = 217/252 (86.11%), Query Frame = 0


Query: 1   LLNPDATEAVQNLLPENVDGNLSALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTR 60
           LL       V+NLLP+ V G+LSALCVWPDQIRHWY+YRWTS LH+IDTPD +CS+ Y+R
Sbjct: 44  LLEAGPAHVVENLLPDYVKGDLSALCVWPDQIRHWYKYRWTSHLHYIDTPDQACSYEYSR 103

Query: 61  DCHDNGGVENMCVAGAVRNFTTQLAHYAEGGSDRRHNLTEALLFLSHFLGDIHQPMHVGF 120
           DCHD  G+++MCV GA++NFT+QL HY EG SDRR+N+TEALLFLSHF+GDIHQPMHVGF
Sbjct: 104 DCHDQHGLKDMCVDGAIQNFTSQLQHYGEGTSDRRYNMTEALLFLSHFMGDIHQPMHVGF 163

Query: 121 TSDEGGNTINLRWYRHKSNLHHVWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHE 180
           TSDEGGNTI+LRWY+HKSNLHHVWDREIILTAL + YDK+  LL +D+++N+T+G+W  +
Sbjct: 164 TSDEGGNTIDLRWYKHKSNLHHVWDREIILTALKENYDKNLDLLQEDLEKNITNGLWHDD 223

Query: 181 IPTWQHCEDLDSCVNKWAEESISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGV 240
           + +W  C DL +C +K+A ESI LACKWGY+GV++G TL+E+YF++R+PIVMKRI +GGV
Sbjct: 224 LSSWTECNDLIACPHKYASESIKLACKWGYKGVKSGETLSEEYFNTRLPIVMKRIVQGGV 283

Query: 241 RLAMLLNRVFSD 253
           RLAM+LNRVFSD
Sbjct: 284 RLAMILNRVFSD 295

[Close]

BLAST of MS006116 vs. TAIR 10
Match: AT4G21585.1 (endonuclease 4 )

HSP 1 Score: 306.6 bits (784), Expect = 1.9e-83
Identity = 134/249 (53.82%), Postives = 185/249 (74.30%), Query Frame = 0


Query: 5   DATEAVQNLLPENVDGNLSALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHD 64
           +   AV+ LLP++ DG+L+++C WPD+I+H +++RWTSPLH++DTPD  C++ Y RDCHD
Sbjct: 44  ETVAAVKKLLPKSADGDLASVCSWPDEIKHHWQWRWTSPLHYVDTPDYRCNYEYCRDCHD 103

Query: 65  NGGVENMCVAGAVRNFTTQLAHYAEGGSDRRH-NLTEALLFLSHFLGDIHQPMHVGFTSD 124
               ++ CV GA+ N+T QL   +E      H NLTEAL+FLSHF+GDIHQP+HVGF  D
Sbjct: 104 THKNQDRCVTGAIFNYTMQLMSASENSDTIVHYNLTEALMFLSHFIGDIHQPLHVGFLGD 163

Query: 125 EGGNTINLRWYRHKSNLHHVWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPT 184
           EGGNTI +RWYR K+NLHHVWD  II +AL  YY+K   L+++ +Q NLT+  W++++P 
Sbjct: 164 EGGNTITVRWYRRKTNLHHVWDNMIIESALKTYYNKSLPLMIEALQANLTND-WSNDVPL 223

Query: 185 WQHCE-DLDSCVNKWAEESISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRL 244
           W+ C+ +  +C N +A ESI+LACK+ Y     GTTL +DYF SR+PIV KR+A+GG+RL
Sbjct: 224 WESCQLNQTACPNPYASESINLACKYAYRNATPGTTLGDDYFLSRLPIVEKRLAQGGIRL 283

Query: 245 AMLLNRVFS 252
           A  LNR+FS
Sbjct: 284 AATLNRIFS 291

[Close]

BLAST of MS006116 vs. TAIR 10
Match: AT1G68290.1 (endonuclease 2 )

HSP 1 Score: 280.4 bits (716), Expect = 1.5e-75
Identity = 125/250 (50.00%), Postives = 177/250 (70.80%), Query Frame = 0


Query: 2   LNPDATEAVQNLLPENVDGNLSALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRD 61
           L+  A +AV+ LLPE+ +G+LS+LC+W D+++  +RY W+SPLH+I+TPD +CS+ Y RD
Sbjct: 44  LDETAAKAVKELLPESAEGDLSSLCLWADRVK--FRYHWSSPLHYINTPD-ACSYQYNRD 103

Query: 62  CHDNGGVENMCVAGAVRNFTTQLAHYAEGGSDR-RHNLTEALLFLSHFLGDIHQPMHVGF 121
           C D  G +  CVAGA+ N+TTQL  Y    S + ++NLTEALLF+SHF+GDIHQP+HV +
Sbjct: 104 CKDESGEKGRCVAGAIYNYTTQLLSYKTAASSQSQYNLTEALLFVSHFMGDIHQPLHVSY 163

Query: 122 TSDEGGNTINLRWYRHKSNLHHVWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHE 181
            SD+GGNTI + WY  K+NLHH+WD  II TA AD Y+     ++  +++N+T   W  +
Sbjct: 164 ASDKGGNTIEVHWYTRKANLHHIWDSNIIETAEADLYNSALEGMVDALKKNITTE-WADQ 223

Query: 182 IPTWQHCEDLDSCVNKWAEESISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGV 241
           +  W+ C    +C + +A E I  AC W Y+GV  G TL ++YF SR+PIV +R+A+GGV
Sbjct: 224 VKRWETCTKKTACPDIYASEGIQAACDWAYKGVTEGDTLEDEYFYSRLPIVYQRLAQGGV 283

Query: 242 RLAMLLNRVF 251
           RLA  LNR+F
Sbjct: 284 RLAATLNRIF 289

[Close]

BLAST of MS006116 vs. TAIR 10
Match: AT4G21600.1 (endonuclease 5 )

HSP 1 Score: 273.5 bits (698), Expect = 1.8e-73
Identity = 124/250 (49.60%), Postives = 173/250 (69.20%), Query Frame = 0


Query: 5   DATEAVQNLLPENVD-GNLSALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCH 64
           D   AV+ LLPE+VD G L+  C WPD+I+   +++WTS LH+++TP+  C++ Y RDCH
Sbjct: 40  DTIAAVKKLLPESVDGGGLADFCSWPDEIKKLSQWQWTSTLHYVNTPEYRCNYEYCRDCH 99

Query: 65  DNGGVENMCVAGAVRNFTTQLAHYAEGGSDRRH-NLTEALLFLSHFLGDIHQPMHVGFTS 124
           D    ++ CV GA+ N+T QL   +E   +  H NLTEALLFLSH++GD+HQP+H GF  
Sbjct: 100 DTHKHKDWCVTGAIFNYTNQLMSASENSQNIVHYNLTEALLFLSHYMGDVHQPLHTGFLG 159

Query: 125 DEGGNTINLRWYRHKSNLHHVWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIP 184
           D GGNTI + WY +KSNLHHVWD  II +AL  YY+     ++Q +Q  L +G W++++P
Sbjct: 160 DLGGNTIIVNWYHNKSNLHHVWDNMIIDSALETYYNSSLPHMIQALQAKLKNG-WSNDVP 219

Query: 185 TWQHCE-DLDSCVNKWAEESISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVR 244
           +W+ C     +C N +A ESI LACK+ Y     GTTL ++YF SR+P+V KR+A+GG+R
Sbjct: 220 SWKSCHFHQKACPNLYASESIDLACKYAYRNATPGTTLGDEYFLSRLPVVEKRLAQGGIR 279

Query: 245 LAMLLNRVFS 252
           LA  LNR+FS
Sbjct: 280 LAATLNRIFS 288

[Close]

BLAST of MS006116 vs. TAIR 10
Match: AT4G21590.1 (endonuclease 3 )

HSP 1 Score: 266.2 bits (679), Expect = 2.9e-71
Identity = 121/249 (48.59%), Postives = 170/249 (68.27%), Query Frame = 0


Query: 5   DATEAVQNLLPENVDGNLSALCVWPDQIRHWYRYRWTSPLHFIDTPDNSCSFLYTRDCHD 64
           D   AV+ LLPE+ +G L+A+C WPD+I+   ++RWTS LHF DTPD  C++ Y+RDC  
Sbjct: 44  DTVVAVKKLLPESANGELAAVCSWPDEIKKLPQWRWTSALHFADTPDYKCNYEYSRDC-- 103

Query: 65  NGGVENMCVAGAVRNFTTQLAHYAEGGSDRRH-NLTEALLFLSHFLGDIHQPMHVGFTSD 124
               ++ CV GA+ N+T QL   +E      H NLTEAL+FLSH++GDIHQP+H GF  D
Sbjct: 104 ---PKDWCVTGAIFNYTNQLMSTSENSQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGD 163

Query: 125 EGGNTINLRWYRHKSNLHHVWDREIILTALADYYDKDSGLLLQDIQRNLTHGIWTHEIPT 184
            GGN I + WY  ++NLH VWD  II +AL  YY+     ++ ++Q  L +G W++++P+
Sbjct: 164 LGGNKIKVHWYNQETNLHRVWDDMIIESALETYYNSSLPRMIHELQAKLKNG-WSNDVPS 223

Query: 185 WQHCE-DLDSCVNKWAEESISLACKWGYEGVEAGTTLAEDYFDSRMPIVMKRIAEGGVRL 244
           W+ C+ +  +C N +A ESI LACK+ Y    AGTTL + YF SR+P+V KR+A+GG+RL
Sbjct: 224 WESCQLNQTACPNPYASESIDLACKYAYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRL 283

Query: 245 AMLLNRVFS 252
           A  LNR+FS
Sbjct: 284 AGTLNRIFS 286

The following BLAST results are available for this feature:

BLAST of MS006116 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140160.1	3.2e-157	99.62	endonuclease 1 [Momordica charantia]	[more]
XP_020221420.1	5.9e-127	77.39	endonuclease 1 [Cajanus cajan] >KYP63013.1 Nuclease PA3 [Cajanus cajan]	[more]
TKY71638.1	4.2e-125	76.63	Endonuclease 1 [Spatholobus suberectus]	[more]
NP_001235325.1	5.5e-125	75.86	endonuclease [Glycine max] >ABP68856.1 endonuclease [Glycine max]	[more]
XP_028203080.1	9.4e-125	75.86	endonuclease 1-like [Glycine soja] >KAG4948408.1 hypothetical protein JHK86_0416...	[more]

BLAST of MS006116 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SXA6	9.5e-112	68.65	Endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=ENDO1 PE=1 SV=1	[more]
F4JJL0	2.7e-82	53.82	Endonuclease 4 OS=Arabidopsis thaliana OX=3702 GN=ENDO4 PE=1 SV=1	[more]
Q9C9G4	2.1e-74	50.00	Endonuclease 2 OS=Arabidopsis thaliana OX=3702 GN=ENDO2 PE=1 SV=1	[more]
F4JJL3	2.5e-72	49.60	Endonuclease 5 OS=Arabidopsis thaliana OX=3702 GN=ENDO5 PE=1 SV=1	[more]
Q8LDW6	4.0e-70	48.59	Endonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=ENDO3 PE=1 SV=1	[more]

BLAST of MS006116 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CEC2	1.5e-157	99.62	Aspergillus nuclease S(1) OS=Momordica charantia OX=3673 GN=LOC111010892 PE=3 SV...	[more]
A0A151T7J6	2.8e-127	77.39	Aspergillus nuclease S(1) OS=Cajanus cajan OX=3821 GN=KK1_017575 PE=3 SV=1	[more]
A5A338	2.7e-125	75.86	Aspergillus nuclease S(1) OS=Glycine max OX=3847 GN=EN1 PE=2 SV=1	[more]
I1MEB5	4.5e-125	75.86	Aspergillus nuclease S(1) OS=Glycine max OX=3847 GN=GLYMA_15G068600 PE=3 SV=1	[more]
A0A445GQE8	4.5e-125	75.86	Aspergillus nuclease S(1) OS=Glycine soja OX=3848 GN=D0Y65_040182 PE=3 SV=1	[more]

BLAST of MS006116 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11190.1	6.7e-113	68.65	bifunctional nuclease i	[more]
AT4G21585.1	1.9e-83	53.82	endonuclease 4	[more]
AT1G68290.1	1.5e-75	50.00	endonuclease 2	[more]
AT4G21600.1	1.8e-73	49.60	endonuclease 5	[more]
AT4G21590.1	2.9e-71	48.59	endonuclease 3	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003154	S1/P1 nuclease	PFAM	PF02265	S1-P1_nuclease	coord: 2..250 e-value: 1.3E-71 score: 241.4
IPR003154	S1/P1 nuclease	PANTHER	PTHR33146	ENDONUCLEASE 4	coord: 1..253
IPR003154	S1/P1 nuclease	CDD	cd11010	S1-P1_nuclease	coord: 1..250 e-value: 2.38783E-74 score: 224.956
IPR008947	Phospholipase C/P1 nuclease domain superfamily	GENE3D	1.10.575.10	P1 Nuclease	coord: 1..254 e-value: 1.2E-91 score: 309.3
IPR008947	Phospholipase C/P1 nuclease domain superfamily	SUPERFAMILY	48537	Phospholipase C/P1 nuclease	coord: 2..248
None	No IPR available	PANTHER	PTHR33146:SF14	ENDONUCLEASE 1	coord: 1..253

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS006116.1	MS006116.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006308	DNA catabolic process
molecular_function	GO:0004519	endonuclease activity
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds
molecular_function	GO:0003676	nucleic acid binding