MS006055 (gene) Bitter gourd (TR) v1

Overview


Name	MS006055
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Procollagen-proline 4-dioxygenase
Location	scaffold254: 3430977 .. 3433845 (+)
RNA-Seq Expression	MS006055
Synteny	MS006055

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAGATTTTGCAGTTGTAGTCTACTATTTTTCTTCTCATTATCGATCTCCTTGCTCCTCCGCCGAGCTTCGAGTTCTTATGCTGGTTCCGCTAGCTCAATCGTCAATCCCGCAAAAGTCAAACAGATTTCATGGAGTCCGCGGTACTTAAATTTCACTCTCGCTGCGTTGTTGTTCTAGTTTCTGAGCTCTGATAATCGACTTCTTCTTTTGGATTAACTCTGTTTCCTCGATGATTTGAATGATTTTACAGGGCTTTTGTATACGAAGGCTTTCTCACGGACTTGGAATGCGATCATATCATCTCGCTTGTGAGTAGAATTAGAATGATTCCTTTTTCTACCACAATGGAAACTCCTTTTTTTTTCTTTCTGTTAATTGTTCTTTTGTTCAAATTAGGCTAAAGCGGAGTTGAAGAGATCTGCTGTTGCGGACAATTTGTCCGGAGAGAGCAAGGTTAGCGAGGTCCGAACTAGCTCTGGGGCGTTTATTCATAAAGCCAAGGTTTGTGCAGAGTTTGCTTCTTGTTTTTTTAAGCTTCTAGCAGAGAAGATAGTGATGTTAAATCTCCATCTAACCTATAGCTTGAACTCTTAGCTTGGAGTAATGTAATTGTTTAATGTACTTTCAAGATTAGTTTGATTCACGAGATCCATTGGTTTAATATTAGTTTGATTTGTGAAGTCTATCTTAATATGAAATGAGTGAGTAAATGACAATCCGGTTATTGTTGAGGATCCTACATTGATAAAAGAAGGGACCTTACATAGGTTTATAACAGACTGGGCTACTCCTCTCCTTGTCAATTGTCAATTGTAATTAGTACTTCAGATCTTCTATCCTGATAGTGTTATAAATCACCACTAGAAACAGAAGTTAGAACTATTGGATCGAAGCAACTTTCATAAGTTATTTTCATCAATTCATACTTGATTTAGAAGGGACAGCTATTATTACTTCTTCATTCTGCACTAGTTTGGATGGCTGATATTTTGGATGTAAATTTTTTGAAGAATATGTTGACAGCCTTAGCAAATTTCGATTTTGCCTATCTTTGATGATTATTGTAGCCGGAAAACATTAAACGTCATCCTTTTGTGTACAGATGGAGACCAATTTTACTTATAAACTTCTAACTTGATGAGGAACTCTATCTCCATTTCTGCTTTGTAAACTTATTGTTCTATATCTCATGTAGGATCCTATTATTTCTGGTATAGAAGACAAAATTGCAGCATGGACATTTCTGCCAAAAGGTATACCATTAGCAGGAGGCACAAATTTCTTGTCAGATCCCATAGTCATTAGCAACTTATCCTAAATTACAGAATGCTTTTTGTTATCCTAAATTAAAGATTGAATTGATTAACAATTGAGTATGGAATTATAGTCTTTTTTACCACAACTCTGGATTTTTACAGGATATAGGCTTTTGCAGCTGAATTCTGAATTTTGGGTGCTTATGTTCTATCCATTGTTATGATTTACATTTTAAAGAAAAATTTTGTGATAATTATATTAAGCTTTAGCGTAGAAAGAAAAAACAGAGAATGAATGGCTGTGTGTCCGATGAGTTCTAGTTCTCATCTTCTGTGTGCACTGGTATAGTTCTATTAACAAGATTTTACTCCATTTAAATTAGTCAACTTAGAAGACATACCCAGTCATAATTGTGAAATACATAATCAAGTGACAGGATCTGAGGAAAAATGTCCTTGCAGCAGGGCAGAAACAGTGTTTGGACTAGGTTCTTTTTCATTTTTTAGTGAGAGAAGAAAACTATGAATGTCAAACTATATTTATATAAGATGTGAAACATTTGACTCGTGCATAAATAGCTTGTTCATGTATTAGTATTACTGGTAAAGTGAAACTTGAATGTGCTTGATTTCAGAAAATGGGGAAGACATTCAAGTGTTGAGATATGAATATGGGCAGAAGTATGATGCACACTTTGATTACTTTTCTGACAAGGTTAATATTGCCCGAGGTGGACATCGAATGGCAACTGTTCTTATGTATCTTTCCAACGTAGAAAAAGGTGGTGAAACTGTGTTTCCTTCTGCAGAGGTCCGTCCCAGTATTTCCCTTCAATCCAATTAATTACAACATTTCTTGCATGCTTGCTTTTTACCGTATTTGGGAATTATCAAGTGTTTTGATTTTTTTTGTACAATATTATTTCCAAACTATTTTTCTCTCGAGTTACCTTACTGCTGGAGGATATATCTGTCTCTACTAAATGACTGCATCATCTAATGCCGTCTTTGAAATTTTAATTAGAATTGGAGAAGTTTCCTGTCTTTGCTGAATGCTGTCACTAAGTGATGATTTTTTTTAATTGAAATTTCAATATTGAATATTTTTGTTCATCCATGTGAGTTCAGGAATCTCAAAGACGCCAGGCTTCTGAAACAAATGAAGATCTCTCAGACTGTGCAAAGAAAGGGATAGCAGGTGAGTCTTTTAAGAAGATAGAAGTCAGTTTTCCAACTCCTGTCCTATTTCTGAAATTAAGTTCGTCGATAGCTGTCGGGATTTCTCTCGTTAACCTCCACCTTTGAAATATTCAGTGAAACCACGGAGAGGCGACGCTCTGCTCTTCTTCAGCCTTCATCCTAATGCTGTTCCAGACACACGTAGTCTGCATGGAGGGTGCCCTGTCATTGAAGGTGAGAAATGGTCAGCAACCAAGTGGATTCATGTCGATTCTTTCGACACGATCTTGAGAGACCATACGAATTGTGCTGATGAGCATGCTAGCTGCGAGAGATGGGCTGAACTCGGCGAGTGCACAAATAACCCGGAGTATATGGTGGGATCTCCCGAGCTTCCTGGCTACTGCAGGAAAAGTTGTAAGGTGTGT

mRNA sequence

ATGGCGAGATTTTGCAGTTGTAGTCTACTATTTTTCTTCTCATTATCGATCTCCTTGCTCCTCCGCCGAGCTTCGAGTTCTTATGCTGGTTCCGCTAGCTCAATCGTCAATCCCGCAAAAGTCAAACAGATTTCATGGAGTCCGCGGGCTTTTGTATACGAAGGCTTTCTCACGGACTTGGAATGCGATCATATCATCTCGCTTGCTAAAGCGGAGTTGAAGAGATCTGCTGTTGCGGACAATTTGTCCGGAGAGAGCAAGGTTAGCGAGGTCCGAACTAGCTCTGGGGCGTTTATTCATAAAGCCAAGGATCCTATTATTTCTGGTATAGAAGACAAAATTGCAGCATGGACATTTCTGCCAAAAGAAAATGGGGAAGACATTCAAGTGTTGAGATATGAATATGGGCAGAAGTATGATGCACACTTTGATTACTTTTCTGACAAGGTTAATATTGCCCGAGGTGGACATCGAATGGCAACTGTTCTTATGTATCTTTCCAACGTAGAAAAAGGTGGTGAAACTGTGTTTCCTTCTGCAGAGGAATCTCAAAGACGCCAGGCTTCTGAAACAAATGAAGATCTCTCAGACTGTGCAAAGAAAGGGATAGCAGTGAAACCACGGAGAGGCGACGCTCTGCTCTTCTTCAGCCTTCATCCTAATGCTGTTCCAGACACACGTAGTCTGCATGGAGGGTGCCCTGTCATTGAAGGTGAGAAATGGTCAGCAACCAAGTGGATTCATGTCGATTCTTTCGACACGATCTTGAGAGACCATACGAATTGTGCTGATGAGCATGCTAGCTGCGAGAGATGGGCTGAACTCGGCGAGTGCACAAATAACCCGGAGTATATGGTGGGATCTCCCGAGCTTCCTGGCTACTGCAGGAAAAGTTGTAAGGTGTGT

Coding sequence (CDS)

ATGGCGAGATTTTGCAGTTGTAGTCTACTATTTTTCTTCTCATTATCGATCTCCTTGCTCCTCCGCCGAGCTTCGAGTTCTTATGCTGGTTCCGCTAGCTCAATCGTCAATCCCGCAAAAGTCAAACAGATTTCATGGAGTCCGCGGGCTTTTGTATACGAAGGCTTTCTCACGGACTTGGAATGCGATCATATCATCTCGCTTGCTAAAGCGGAGTTGAAGAGATCTGCTGTTGCGGACAATTTGTCCGGAGAGAGCAAGGTTAGCGAGGTCCGAACTAGCTCTGGGGCGTTTATTCATAAAGCCAAGGATCCTATTATTTCTGGTATAGAAGACAAAATTGCAGCATGGACATTTCTGCCAAAAGAAAATGGGGAAGACATTCAAGTGTTGAGATATGAATATGGGCAGAAGTATGATGCACACTTTGATTACTTTTCTGACAAGGTTAATATTGCCCGAGGTGGACATCGAATGGCAACTGTTCTTATGTATCTTTCCAACGTAGAAAAAGGTGGTGAAACTGTGTTTCCTTCTGCAGAGGAATCTCAAAGACGCCAGGCTTCTGAAACAAATGAAGATCTCTCAGACTGTGCAAAGAAAGGGATAGCAGTGAAACCACGGAGAGGCGACGCTCTGCTCTTCTTCAGCCTTCATCCTAATGCTGTTCCAGACACACGTAGTCTGCATGGAGGGTGCCCTGTCATTGAAGGTGAGAAATGGTCAGCAACCAAGTGGATTCATGTCGATTCTTTCGACACGATCTTGAGAGACCATACGAATTGTGCTGATGAGCATGCTAGCTGCGAGAGATGGGCTGAACTCGGCGAGTGCACAAATAACCCGGAGTATATGGTGGGATCTCCCGAGCTTCCTGGCTACTGCAGGAAAAGTTGTAAGGTGTGT

Protein sequence

MARFCSCSLLFFFSLSISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHIISLAKAELKRSAVADNLSGESKVSEVRTSSGAFIHKAKDPIISGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFSDKVNIARGGHRMATVLMYLSNVEKGGETVFPSAEESQRRQASETNEDLSDCAKKGIAVKPRRGDALLFFSLHPNAVPDTRSLHGGCPVIEGEKWSATKWIHVDSFDTILRDHTNCADEHASCERWAELGECTNNPEYMVGSPELPGYCRKSCKVC

Homology

[Close]

BLAST of MS006055 vs. NCBI nr
Match: XP_022137761.1 (probable prolyl 4-hydroxylase 4 [Momordica charantia])

HSP 1 Score: 613.6 bits (1581), Expect = 9.1e-172
Identity = 301/302 (99.67%), Postives = 301/302 (99.67%), Query Frame = 0


Query: 1   MARFCSCSLLFFFSLSISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60
           MARFCSCSLLFFFSLSISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL
Sbjct: 1   MARFCSCSLLFFFSLSISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60

Query: 61  ECDHIISLAKAELKRSAVADNLSGESKVSEVRTSSGAFIHKAKDPIISGIEDKIAAWTFL 120
           ECDHIISLAKAELKRSAVADNLSGESKVSEVRTSSGAFIHKAKDPIISGIEDKIAAWTFL
Sbjct: 61  ECDHIISLAKAELKRSAVADNLSGESKVSEVRTSSGAFIHKAKDPIISGIEDKIAAWTFL 120

Query: 121 PKENGEDIQVLRYEYGQKYDAHFDYFSDKVNIARGGHRMATVLMYLSNVEKGGETVFPSA 180
           PKENGEDIQVLRYEYGQKYDAHFDYFSDKVNIARGGHRMATVLMYLSNVEKGGETVFPSA
Sbjct: 121 PKENGEDIQVLRYEYGQKYDAHFDYFSDKVNIARGGHRMATVLMYLSNVEKGGETVFPSA 180

Query: 181 EESQRRQASETNEDLSDCAKKGIAVKPRRGDALLFFSLHPNAVPDTRSLHGGCPVIEGEK 240
           EESQRRQASETNEDLSDCAKKGIAVKPRRGDALLFFSLHPNAVPDT SLHGGCPVIEGEK
Sbjct: 181 EESQRRQASETNEDLSDCAKKGIAVKPRRGDALLFFSLHPNAVPDTCSLHGGCPVIEGEK 240

Query: 241 WSATKWIHVDSFDTILRDHTNCADEHASCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300
           WSATKWIHVDSFDTILRDHTNCADEHASCERWAELGECTNNPEYMVGSPELPGYCRKSCK
Sbjct: 241 WSATKWIHVDSFDTILRDHTNCADEHASCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300

Query: 301 VC 303
           VC
Sbjct: 301 VC 302

[Close]

BLAST of MS006055 vs. NCBI nr
Match: XP_022973641.1 (probable prolyl 4-hydroxylase 4 [Cucurbita maxima])

HSP 1 Score: 589.7 bits (1519), Expect = 1.4e-164
Identity = 284/302 (94.04%), Postives = 297/302 (98.34%), Query Frame = 0


Query: 1   MARFCSCSLLFFFSLSISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60
           MA+FCSC+LLF  S+SISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL
Sbjct: 1   MAKFCSCNLLFILSISISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60

Query: 61  ECDHIISLAKAELKRSAVADNLSGESKVSEVRTSSGAFIHKAKDPIISGIEDKIAAWTFL 120
           ECDH+ISLAKAELKRSAVAD+LSGESKVSEVRTSSGAFIHKAKDPI+SGIEDKIAAWTFL
Sbjct: 61  ECDHLISLAKAELKRSAVADHLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 120

Query: 121 PKENGEDIQVLRYEYGQKYDAHFDYFSDKVNIARGGHRMATVLMYLSNVEKGGETVFPSA 180
           PKENGEDIQVLRYEYGQKYDAHFDYF+DKVNIARGGHRMATVLMYLSNVEKGGETVFP+A
Sbjct: 121 PKENGEDIQVLRYEYGQKYDAHFDYFTDKVNIARGGHRMATVLMYLSNVEKGGETVFPNA 180

Query: 181 EESQRRQASETNEDLSDCAKKGIAVKPRRGDALLFFSLHPNAVPDTRSLHGGCPVIEGEK 240
           EESQRRQASETNEDLSDCAKKGIAVKPR+GDALLFFSLHPNAVPDT+SLHGGCPVIEGEK
Sbjct: 181 EESQRRQASETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAVPDTKSLHGGCPVIEGEK 240

Query: 241 WSATKWIHVDSFDTILRDHTNCADEHASCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300
           WSATKWIHVDSFDTI+ DHT+C D +ASCERWAELGECTNNPEYMVGSPELPGYCRKSCK
Sbjct: 241 WSATKWIHVDSFDTIVSDHTSCVDNNASCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300

Query: 301 VC 303
           VC
Sbjct: 301 VC 302

[Close]

BLAST of MS006055 vs. NCBI nr
Match: KAG6597483.1 (putative prolyl 4-hydroxylase 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 588.6 bits (1516), Expect = 3.1e-164
Identity = 283/302 (93.71%), Postives = 297/302 (98.34%), Query Frame = 0


Query: 1   MARFCSCSLLFFFSLSISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60
           MA+FCSC+LLF  S+SISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL
Sbjct: 1   MAKFCSCNLLFILSISISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60

Query: 61  ECDHIISLAKAELKRSAVADNLSGESKVSEVRTSSGAFIHKAKDPIISGIEDKIAAWTFL 120
           ECDH+ISLAKAELKRSAVAD+LSGESKVSEVRTSSGAFIHKAKDPI+SGIEDKIAAWTFL
Sbjct: 61  ECDHLISLAKAELKRSAVADHLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 120

Query: 121 PKENGEDIQVLRYEYGQKYDAHFDYFSDKVNIARGGHRMATVLMYLSNVEKGGETVFPSA 180
           PKENGEDIQVLRYEYGQKYDAH+DYF+DKVNIARGGHRMATVLMYLSNVEKGGETVFP+A
Sbjct: 121 PKENGEDIQVLRYEYGQKYDAHYDYFTDKVNIARGGHRMATVLMYLSNVEKGGETVFPNA 180

Query: 181 EESQRRQASETNEDLSDCAKKGIAVKPRRGDALLFFSLHPNAVPDTRSLHGGCPVIEGEK 240
           EESQRRQASETNEDLSDCAKKGIAVKPR+GDALLFFSLHPNAVPDT+SLHGGCPVIEGEK
Sbjct: 181 EESQRRQASETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAVPDTKSLHGGCPVIEGEK 240

Query: 241 WSATKWIHVDSFDTILRDHTNCADEHASCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300
           WSATKWIHVDSFDTI+ DHT+C D +ASCERWAELGECTNNPEYMVGSPELPGYCRKSCK
Sbjct: 241 WSATKWIHVDSFDTIVSDHTSCVDNNASCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300

Query: 301 VC 303
           VC
Sbjct: 301 VC 302

[Close]

BLAST of MS006055 vs. NCBI nr
Match: XP_022954026.1 (probable prolyl 4-hydroxylase 4 [Cucurbita moschata])

HSP 1 Score: 587.4 bits (1513), Expect = 7.0e-164
Identity = 282/302 (93.38%), Postives = 297/302 (98.34%), Query Frame = 0


Query: 1   MARFCSCSLLFFFSLSISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60
           MA+FCSC+LLF  S+SISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL
Sbjct: 1   MAKFCSCNLLFILSISISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60

Query: 61  ECDHIISLAKAELKRSAVADNLSGESKVSEVRTSSGAFIHKAKDPIISGIEDKIAAWTFL 120
           ECDH+ISLAKAELKRSAVAD+LSGESKVSEVRTSSGAFIHKAKDPI+SGIEDKIAAWTFL
Sbjct: 61  ECDHLISLAKAELKRSAVADHLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 120

Query: 121 PKENGEDIQVLRYEYGQKYDAHFDYFSDKVNIARGGHRMATVLMYLSNVEKGGETVFPSA 180
           PKENGEDIQVLRYEYGQKYDAH+DYF+DKVNIARGGHRMATVLMYLSNVEKGGETVFP+A
Sbjct: 121 PKENGEDIQVLRYEYGQKYDAHYDYFTDKVNIARGGHRMATVLMYLSNVEKGGETVFPNA 180

Query: 181 EESQRRQASETNEDLSDCAKKGIAVKPRRGDALLFFSLHPNAVPDTRSLHGGCPVIEGEK 240
           EESQRRQASETNEDLSDCAKKGIAVKPR+GDALLFF+LHPNAVPDT+SLHGGCPVIEGEK
Sbjct: 181 EESQRRQASETNEDLSDCAKKGIAVKPRKGDALLFFNLHPNAVPDTKSLHGGCPVIEGEK 240

Query: 241 WSATKWIHVDSFDTILRDHTNCADEHASCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300
           WSATKWIHVDSFDTI+ DHT+C D +ASCERWAELGECTNNPEYMVGSPELPGYCRKSCK
Sbjct: 241 WSATKWIHVDSFDTIVSDHTSCVDNNASCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300

Query: 301 VC 303
           VC
Sbjct: 301 VC 302

[Close]

BLAST of MS006055 vs. NCBI nr
Match: XP_023539189.1 (probable prolyl 4-hydroxylase 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 587.0 bits (1512), Expect = 9.1e-164
Identity = 282/302 (93.38%), Postives = 296/302 (98.01%), Query Frame = 0


Query: 1   MARFCSCSLLFFFSLSISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60
           MA+FCSC+LLF  S+SISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL
Sbjct: 1   MAKFCSCNLLFILSISISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60

Query: 61  ECDHIISLAKAELKRSAVADNLSGESKVSEVRTSSGAFIHKAKDPIISGIEDKIAAWTFL 120
           ECDH+ISLAKAELKRSAVAD+LSGESKVSEVRTSSGAFIHKAKDPI+SGIEDKIAAWTFL
Sbjct: 61  ECDHLISLAKAELKRSAVADHLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 120

Query: 121 PKENGEDIQVLRYEYGQKYDAHFDYFSDKVNIARGGHRMATVLMYLSNVEKGGETVFPSA 180
           PKENGEDIQVLRYEYGQKYD H+DYF+DKVNIARGGHRMATVLMYLSNVEKGGETVFP+A
Sbjct: 121 PKENGEDIQVLRYEYGQKYDVHYDYFTDKVNIARGGHRMATVLMYLSNVEKGGETVFPNA 180

Query: 181 EESQRRQASETNEDLSDCAKKGIAVKPRRGDALLFFSLHPNAVPDTRSLHGGCPVIEGEK 240
           EESQRRQASETNEDLSDCAKKGIAVKPR+GDALLFFSLHPNAVPDT+SLHGGCPVIEGEK
Sbjct: 181 EESQRRQASETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAVPDTKSLHGGCPVIEGEK 240

Query: 241 WSATKWIHVDSFDTILRDHTNCADEHASCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300
           WSATKWIHVDSFDTI+ DHT+C D +ASCERWAELGECTNNPEYMVGSPELPGYCRKSCK
Sbjct: 241 WSATKWIHVDSFDTIVSDHTSCVDNNASCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300

Query: 301 VC 303
           VC
Sbjct: 301 VC 302

[Close]

BLAST of MS006055 vs. ExPASy Swiss-Prot
Match: Q8LAN3 (Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 2.0e-129
Identity = 225/294 (76.53%), Postives = 248/294 (84.35%), Query Frame = 0


Query: 9   LLFFFSLSISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHIISL 68
           LL  F    S+LL ++S+S   S+S  VNP+KVKQ+S  PRAFVYEGFLT+LECDH++SL
Sbjct: 6   LLISFFAIFSVLL-QSSTSLISSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHMVSL 65

Query: 69  AKAELKRSAVADNLSGESKVSEVRTSSGAFIHKAKDPIISGIEDKIAAWTFLPKENGEDI 128
           AKA LKRSAVADN SGESK SEVRTSSG FI K KDPI+SGIEDKI+ WTFLPKENGEDI
Sbjct: 66  AKASLKRSAVADNDSGESKFSEVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDI 125

Query: 129 QVLRYEYGQKYDAHFDYFSDKVNIARGGHRMATVLMYLSNVEKGGETVFPSAEESQRRQA 188
           QVLRYE+GQKYDAHFDYF DKVNI RGGHRMAT+LMYLSNV KGGETVFP AE   RR  
Sbjct: 126 QVLRYEHGQKYDAHFDYFHDKVNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPSRRVL 185

Query: 189 SETNEDLSDCAKKGIAVKPRRGDALLFFSLHPNAVPDTRSLHGGCPVIEGEKWSATKWIH 248
           SE  EDLSDCAK+GIAVKPR+GDALLFF+LHP+A+PD  SLHGGCPVIEGEKWSATKWIH
Sbjct: 186 SENKEDLSDCAKRGIAVKPRKGDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATKWIH 245

Query: 249 VDSFDTILRDHTNCADEHASCERWAELGECTNNPEYMVGSPELPGYCRKSCKVC 303
           VDSFD I+    NC D + SCERWA LGECT NPEYMVG+ ELPGYCR+SCK C
Sbjct: 246 VDSFDRIVTPSGNCTDMNESCERWAVLGECTKNPEYMVGTTELPGYCRRSCKAC 298

[Close]

BLAST of MS006055 vs. ExPASy Swiss-Prot
Match: F4JAU3 (Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 1.3e-125
Identity = 216/294 (73.47%), Postives = 249/294 (84.69%), Query Frame = 0


Query: 9   LLFFFSLSISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHIISL 68
           LL F  ++I L+L ++S+    S SSI+NP+KVKQ+S  PRAFVYEGFLTDLECDH+ISL
Sbjct: 8   LLLF--VAILLVLLQSSTCLISSPSSIINPSKVKQVSSKPRAFVYEGFLTDLECDHLISL 67

Query: 69  AKAELKRSAVADNLSGESKVSEVRTSSGAFIHKAKDPIISGIEDKIAAWTFLPKENGEDI 128
           AK  L+RSAVADN +GES+VS+VRTSSG FI K KDPI+SGIEDK++ WTFLPKENGED+
Sbjct: 68  AKENLQRSAVADNDNGESQVSDVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPKENGEDL 127

Query: 129 QVLRYEYGQKYDAHFDYFSDKVNIARGGHRMATVLMYLSNVEKGGETVFPSAEESQRRQA 188
           QVLRYE+GQKYDAHFDYF DKVNIARGGHR+ATVL+YLSNV KGGETVFP A+E  RR  
Sbjct: 128 QVLRYEHGQKYDAHFDYFHDKVNIARGGHRIATVLLYLSNVTKGGETVFPDAQEFSRRSL 187

Query: 189 SETNEDLSDCAKKGIAVKPRRGDALLFFSLHPNAVPDTRSLHGGCPVIEGEKWSATKWIH 248
           SE  +DLSDCAKKGIAVKP++G+ALLFF+L  +A+PD  SLHGGCPVIEGEKWSATKWIH
Sbjct: 188 SENKDDLSDCAKKGIAVKPKKGNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIH 247

Query: 249 VDSFDTILRDHTNCADEHASCERWAELGECTNNPEYMVGSPELPGYCRKSCKVC 303
           VDSFD IL    NC D + SCERWA LGEC  NPEYMVG+PE+PG CR+SCK C
Sbjct: 248 VDSFDKILTHDGNCTDVNESCERWAVLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

[Close]

BLAST of MS006055 vs. ExPASy Swiss-Prot
Match: Q8L970 (Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 3.3e-100
Identity = 182/313 (58.15%), Postives = 229/313 (73.16%), Query Frame = 0


Query: 4   FCSCSLLFFFSLSI-----SLLLRRASSSYAG-------SASSI-VNPAKVKQISWSPRA 63
           F + SL F F+L +     +  L R+S++  G       SASS   +P +V Q+SW+PR 
Sbjct: 6   FLAFSLCFLFTLPLISSAPNRFLTRSSNTRDGSVIKMKTSASSFGFDPTRVTQLSWTPRV 65

Query: 64  FVYEGFLTDLECDHIISLAKAELKRSAVADNLSGESKVSEVRTSSGAFIHKAKDPIISGI 123
           F+YEGFL+D ECDH I LAK +L++S VADN SGES  SEVRTSSG F+ K +D I+S +
Sbjct: 66  FLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSSGMFLSKRQDDIVSNV 125

Query: 124 EDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFSDKVNIARGGHRMATVLMYLSNVE 183
           E K+AAWTFLP+ENGE +Q+L YE GQKY+ HFDYF D+ N+  GGHR+ATVLMYLSNVE
Sbjct: 126 EAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGGHRIATVLMYLSNVE 185

Query: 184 KGGETVFPSAEESQRRQASETNED-LSDCAKKGIAVKPRRGDALLFFSLHPNAVPDTRSL 243
           KGGETVFP      + +A++  +D  ++CAK+G AVKPR+GDALLFF+LHPNA  D+ SL
Sbjct: 186 KGGETVFP----MWKGKATQLKDDSWTECAKQGYAVKPRKGDALLFFNLHPNATTDSNSL 245

Query: 244 HGGCPVIEGEKWSATKWIHVDSFDTILRDHTNCADEHASCERWAELGECTNNPEYMVGSP 303
           HG CPV+EGEKWSAT+WIHV SF+      + C DE+ SCE+WA+ GEC  NP YMVGS 
Sbjct: 246 HGSCPVVEGEKWSATRWIHVKSFERAFNKQSGCMDENVSCEKWAKAGECQKNPTYMVGSD 305

[Close]

BLAST of MS006055 vs. ExPASy Swiss-Prot
Match: F4J0A8 (Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 6.7e-93
Identity = 171/293 (58.36%), Postives = 212/293 (72.35%), Query Frame = 0


Query: 11  FFFSLSISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHIISLAK 70
           +F + S+SLLL     S   S S  V+P ++ Q+SW+PRAF+Y+GFL+D ECDH+I LAK
Sbjct: 5   YFLAFSLSLLL---IFSQISSFSFSVDPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAK 64

Query: 71  AELKRS-AVADNLSGESKVSEVRTSSGAFIHKAKDPIISGIEDKIAAWTFLPKENGEDIQ 130
            +L++S  VAD  SGES+ SEVRTSSG F+ K +D I++ +E K+AAWTFLP+ENGE +Q
Sbjct: 65  GKLEKSMVVADVDSGESEDSEVRTSSGMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQ 124

Query: 131 VLRYEYGQKYDAHFDYFSDKVNIARGGHRMATVLMYLSNVEKGGETVFPSAEESQRRQAS 190
           +L YE GQKYD HFDYF DK  +  GGHR+ATVLMYLSNV KGGETVFP+    + +   
Sbjct: 125 ILHYENGQKYDPHFDYFYDKKALELGGHRIATVLMYLSNVTKGGETVFPN---WKGKTPQ 184

Query: 191 ETNEDLSDCAKKGIAVKPRRGDALLFFSLHPNAVPDTRSLHGGCPVIEGEKWSATKWIHV 250
             ++  S CAK+G AVKPR+GDALLFF+LH N   D  SLHG CPVIEGEKWSAT+WIHV
Sbjct: 185 LKDDSWSKCAKQGYAVKPRKGDALLFFNLHLNGTTDPNSLHGSCPVIEGEKWSATRWIHV 244

Query: 251 DSFDTILRDHTNCADEHASCERWAELGECTNNPEYMVGSPELPGYCRKSCKVC 303
            SF    +    C D+H SC+ WA+ GEC  NP YMVGS    G+CRKSCK C
Sbjct: 245 RSFG---KKKLVCVDDHESCQEWADAGECEKNPMYMVGSETSLGFCRKSCKAC 288

[Close]

BLAST of MS006055 vs. ExPASy Swiss-Prot
Match: Q9LN20 (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 2.0e-65
Identity = 117/209 (55.98%), Postives = 155/209 (74.16%), Query Frame = 0


Query: 44  ISWSPRAFVYEGFLTDLECDHIISLAKAELKRSAVADNLSGESKVSEVRTSSGAFIHKAK 103
           +SW PRAFVY  FL+  EC+++ISLAK  + +S V D+ +G+SK S VRTSSG F+ + +
Sbjct: 79  LSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETGKSKDSRVRTSSGTFLRRGR 138

Query: 104 DPIISGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFSDKVNIARGGHRMATVL 163
           D II  IE +IA +TF+P ++GE +QVL YE GQKY+ H+DYF D+ N   GG RMAT+L
Sbjct: 139 DKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYDYFVDEFNTKNGGQRMATML 198

Query: 164 MYLSNVEKGGETVFPSAEESQRRQASETNEDLSDCAKKGIAVKPRRGDALLFFSLHPNAV 223
           MYLS+VE+GGETVFP+A  +    +     +LS+C KKG++VKPR GDALLF+S+ P+A 
Sbjct: 199 MYLSDVEEGGETVFPAA--NMNFSSVPWYNELSECGKKGLSVKPRMGDALLFWSMRPDAT 258

Query: 224 PDTRSLHGGCPVIEGEKWSATKWIHVDSF 253
            D  SLHGGCPVI G KWS+TKW+HV  +
Sbjct: 259 LDPTSLHGGCPVIRGNKWSSTKWMHVGEY 285

[Close]

BLAST of MS006055 vs. ExPASy TrEMBL
Match: A0A6J1C7M6 (Procollagen-proline 4-dioxygenase OS=Momordica charantia OX=3673 GN=LOC111009125 PE=3 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 4.4e-172
Identity = 301/302 (99.67%), Postives = 301/302 (99.67%), Query Frame = 0


Query: 1   MARFCSCSLLFFFSLSISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60
           MARFCSCSLLFFFSLSISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL
Sbjct: 1   MARFCSCSLLFFFSLSISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60

Query: 61  ECDHIISLAKAELKRSAVADNLSGESKVSEVRTSSGAFIHKAKDPIISGIEDKIAAWTFL 120
           ECDHIISLAKAELKRSAVADNLSGESKVSEVRTSSGAFIHKAKDPIISGIEDKIAAWTFL
Sbjct: 61  ECDHIISLAKAELKRSAVADNLSGESKVSEVRTSSGAFIHKAKDPIISGIEDKIAAWTFL 120

Query: 121 PKENGEDIQVLRYEYGQKYDAHFDYFSDKVNIARGGHRMATVLMYLSNVEKGGETVFPSA 180
           PKENGEDIQVLRYEYGQKYDAHFDYFSDKVNIARGGHRMATVLMYLSNVEKGGETVFPSA
Sbjct: 121 PKENGEDIQVLRYEYGQKYDAHFDYFSDKVNIARGGHRMATVLMYLSNVEKGGETVFPSA 180

Query: 181 EESQRRQASETNEDLSDCAKKGIAVKPRRGDALLFFSLHPNAVPDTRSLHGGCPVIEGEK 240
           EESQRRQASETNEDLSDCAKKGIAVKPRRGDALLFFSLHPNAVPDT SLHGGCPVIEGEK
Sbjct: 181 EESQRRQASETNEDLSDCAKKGIAVKPRRGDALLFFSLHPNAVPDTCSLHGGCPVIEGEK 240

Query: 241 WSATKWIHVDSFDTILRDHTNCADEHASCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300
           WSATKWIHVDSFDTILRDHTNCADEHASCERWAELGECTNNPEYMVGSPELPGYCRKSCK
Sbjct: 241 WSATKWIHVDSFDTILRDHTNCADEHASCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300

Query: 301 VC 303
           VC
Sbjct: 301 VC 302

[Close]

BLAST of MS006055 vs. ExPASy TrEMBL
Match: A0A6J1I971 (Procollagen-proline 4-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111472232 PE=3 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 6.8e-165
Identity = 284/302 (94.04%), Postives = 297/302 (98.34%), Query Frame = 0


Query: 1   MARFCSCSLLFFFSLSISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60
           MA+FCSC+LLF  S+SISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL
Sbjct: 1   MAKFCSCNLLFILSISISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60

Query: 61  ECDHIISLAKAELKRSAVADNLSGESKVSEVRTSSGAFIHKAKDPIISGIEDKIAAWTFL 120
           ECDH+ISLAKAELKRSAVAD+LSGESKVSEVRTSSGAFIHKAKDPI+SGIEDKIAAWTFL
Sbjct: 61  ECDHLISLAKAELKRSAVADHLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 120

Query: 121 PKENGEDIQVLRYEYGQKYDAHFDYFSDKVNIARGGHRMATVLMYLSNVEKGGETVFPSA 180
           PKENGEDIQVLRYEYGQKYDAHFDYF+DKVNIARGGHRMATVLMYLSNVEKGGETVFP+A
Sbjct: 121 PKENGEDIQVLRYEYGQKYDAHFDYFTDKVNIARGGHRMATVLMYLSNVEKGGETVFPNA 180

Query: 181 EESQRRQASETNEDLSDCAKKGIAVKPRRGDALLFFSLHPNAVPDTRSLHGGCPVIEGEK 240
           EESQRRQASETNEDLSDCAKKGIAVKPR+GDALLFFSLHPNAVPDT+SLHGGCPVIEGEK
Sbjct: 181 EESQRRQASETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAVPDTKSLHGGCPVIEGEK 240

Query: 241 WSATKWIHVDSFDTILRDHTNCADEHASCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300
           WSATKWIHVDSFDTI+ DHT+C D +ASCERWAELGECTNNPEYMVGSPELPGYCRKSCK
Sbjct: 241 WSATKWIHVDSFDTIVSDHTSCVDNNASCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300

Query: 301 VC 303
           VC
Sbjct: 301 VC 302

[Close]

BLAST of MS006055 vs. ExPASy TrEMBL
Match: A0A6J1GPQ8 (Procollagen-proline 4-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111456381 PE=3 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 3.4e-164
Identity = 282/302 (93.38%), Postives = 297/302 (98.34%), Query Frame = 0


Query: 1   MARFCSCSLLFFFSLSISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60
           MA+FCSC+LLF  S+SISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL
Sbjct: 1   MAKFCSCNLLFILSISISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60

Query: 61  ECDHIISLAKAELKRSAVADNLSGESKVSEVRTSSGAFIHKAKDPIISGIEDKIAAWTFL 120
           ECDH+ISLAKAELKRSAVAD+LSGESKVSEVRTSSGAFIHKAKDPI+SGIEDKIAAWTFL
Sbjct: 61  ECDHLISLAKAELKRSAVADHLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 120

Query: 121 PKENGEDIQVLRYEYGQKYDAHFDYFSDKVNIARGGHRMATVLMYLSNVEKGGETVFPSA 180
           PKENGEDIQVLRYEYGQKYDAH+DYF+DKVNIARGGHRMATVLMYLSNVEKGGETVFP+A
Sbjct: 121 PKENGEDIQVLRYEYGQKYDAHYDYFTDKVNIARGGHRMATVLMYLSNVEKGGETVFPNA 180

Query: 181 EESQRRQASETNEDLSDCAKKGIAVKPRRGDALLFFSLHPNAVPDTRSLHGGCPVIEGEK 240
           EESQRRQASETNEDLSDCAKKGIAVKPR+GDALLFF+LHPNAVPDT+SLHGGCPVIEGEK
Sbjct: 181 EESQRRQASETNEDLSDCAKKGIAVKPRKGDALLFFNLHPNAVPDTKSLHGGCPVIEGEK 240

Query: 241 WSATKWIHVDSFDTILRDHTNCADEHASCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300
           WSATKWIHVDSFDTI+ DHT+C D +ASCERWAELGECTNNPEYMVGSPELPGYCRKSCK
Sbjct: 241 WSATKWIHVDSFDTIVSDHTSCVDNNASCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300

Query: 301 VC 303
           VC
Sbjct: 301 VC 302

[Close]

BLAST of MS006055 vs. ExPASy TrEMBL
Match: A0A1S3AWU7 (Procollagen-proline 4-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103483779 PE=3 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 1.0e-160
Identity = 278/302 (92.05%), Postives = 291/302 (96.36%), Query Frame = 0


Query: 1   MARFCSCSLLFFFSLSISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60
           MA F   +LLF F+L+IS LLRRAS+SYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL
Sbjct: 1   MAEFLRFNLLFLFTLTISYLLRRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60

Query: 61  ECDHIISLAKAELKRSAVADNLSGESKVSEVRTSSGAFIHKAKDPIISGIEDKIAAWTFL 120
           ECDH+ISLAKAELKRS+VADNLSGESKVSEVRTSSGAFIHKAKDPI+SGIEDKIAAWTFL
Sbjct: 61  ECDHLISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 120

Query: 121 PKENGEDIQVLRYEYGQKYDAHFDYFSDKVNIARGGHRMATVLMYLSNVEKGGETVFPSA 180
           PKENGEDIQVLRYEYGQKYDAHFDYF+DKVNIARGGHRMATVLMYLS+VEKGGETVFPSA
Sbjct: 121 PKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA 180

Query: 181 EESQRRQASETNEDLSDCAKKGIAVKPRRGDALLFFSLHPNAVPDTRSLHGGCPVIEGEK 240
           EESQRRQASETN+DLSDCAKKGIAVKPR+GDALLFFSLHPNA+PDT SLHGGCPVIEGEK
Sbjct: 181 EESQRRQASETNQDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEK 240

Query: 241 WSATKWIHVDSFDTILRDHTNCADEHASCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300
           WSATKWIHVDSFDTI RDHTNC DE+ SCERWAELGECTNNPEYMVGSPELPGYCRKSCK
Sbjct: 241 WSATKWIHVDSFDTIARDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300

Query: 301 VC 303
            C
Sbjct: 301 AC 302

[Close]

BLAST of MS006055 vs. ExPASy TrEMBL
Match: A0A0A0L5Q6 (Procollagen-proline 4-dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_3G150820 PE=3 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 4.7e-158
Identity = 273/302 (90.40%), Postives = 289/302 (95.70%), Query Frame = 0


Query: 1   MARFCSCSLLFFFSLSISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60
           MA     +LLF F+LSIS LL+RAS+SYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL
Sbjct: 37  MAELLCFNLLFLFTLSISFLLQRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 96

Query: 61  ECDHIISLAKAELKRSAVADNLSGESKVSEVRTSSGAFIHKAKDPIISGIEDKIAAWTFL 120
           ECDH+ISLAKAELKRS+VADNLSG+SKVSEVRTSSGAFIHKAKDPI+SGIEDKIAAWTFL
Sbjct: 97  ECDHLISLAKAELKRSSVADNLSGKSKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 156

Query: 121 PKENGEDIQVLRYEYGQKYDAHFDYFSDKVNIARGGHRMATVLMYLSNVEKGGETVFPSA 180
           PK+NGEDIQVLRYEYGQKYDAHFDYF+DKVNIARGGHRMATVLMYLS+VEKGGETVFPSA
Sbjct: 157 PKDNGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA 216

Query: 181 EESQRRQASETNEDLSDCAKKGIAVKPRRGDALLFFSLHPNAVPDTRSLHGGCPVIEGEK 240
           EESQRRQASETNEDLSDCAKKGIAVKPR+GDALLFFSLHPNA+PDT SLHGGCPVIEGEK
Sbjct: 217 EESQRRQASETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEK 276

Query: 241 WSATKWIHVDSFDTILRDHTNCADEHASCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300
           WSATKWI VDSFD ++RDHTNC DE+ SCERWAELGECTNNPEYMVGSPELPGYCRKSCK
Sbjct: 277 WSATKWIRVDSFDMVVRDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCK 336

Query: 301 VC 303
            C
Sbjct: 337 AC 338

[Close]

BLAST of MS006055 vs. TAIR 10
Match: AT5G18900.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 463.4 bits (1191), Expect = 1.4e-130
Identity = 225/294 (76.53%), Postives = 248/294 (84.35%), Query Frame = 0


Query: 9   LLFFFSLSISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHIISL 68
           LL  F    S+LL ++S+S   S+S  VNP+KVKQ+S  PRAFVYEGFLT+LECDH++SL
Sbjct: 6   LLISFFAIFSVLL-QSSTSLISSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHMVSL 65

Query: 69  AKAELKRSAVADNLSGESKVSEVRTSSGAFIHKAKDPIISGIEDKIAAWTFLPKENGEDI 128
           AKA LKRSAVADN SGESK SEVRTSSG FI K KDPI+SGIEDKI+ WTFLPKENGEDI
Sbjct: 66  AKASLKRSAVADNDSGESKFSEVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDI 125

Query: 129 QVLRYEYGQKYDAHFDYFSDKVNIARGGHRMATVLMYLSNVEKGGETVFPSAEESQRRQA 188
           QVLRYE+GQKYDAHFDYF DKVNI RGGHRMAT+LMYLSNV KGGETVFP AE   RR  
Sbjct: 126 QVLRYEHGQKYDAHFDYFHDKVNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPSRRVL 185

Query: 189 SETNEDLSDCAKKGIAVKPRRGDALLFFSLHPNAVPDTRSLHGGCPVIEGEKWSATKWIH 248
           SE  EDLSDCAK+GIAVKPR+GDALLFF+LHP+A+PD  SLHGGCPVIEGEKWSATKWIH
Sbjct: 186 SENKEDLSDCAKRGIAVKPRKGDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATKWIH 245

Query: 249 VDSFDTILRDHTNCADEHASCERWAELGECTNNPEYMVGSPELPGYCRKSCKVC 303
           VDSFD I+    NC D + SCERWA LGECT NPEYMVG+ ELPGYCR+SCK C
Sbjct: 246 VDSFDRIVTPSGNCTDMNESCERWAVLGECTKNPEYMVGTTELPGYCRRSCKAC 298

[Close]

BLAST of MS006055 vs. TAIR 10
Match: AT3G06300.1 (P4H isoform 2 )

HSP 1 Score: 450.7 bits (1158), Expect = 9.5e-127
Identity = 216/294 (73.47%), Postives = 249/294 (84.69%), Query Frame = 0


Query: 9   LLFFFSLSISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHIISL 68
           LL F  ++I L+L ++S+    S SSI+NP+KVKQ+S  PRAFVYEGFLTDLECDH+ISL
Sbjct: 8   LLLF--VAILLVLLQSSTCLISSPSSIINPSKVKQVSSKPRAFVYEGFLTDLECDHLISL 67

Query: 69  AKAELKRSAVADNLSGESKVSEVRTSSGAFIHKAKDPIISGIEDKIAAWTFLPKENGEDI 128
           AK  L+RSAVADN +GES+VS+VRTSSG FI K KDPI+SGIEDK++ WTFLPKENGED+
Sbjct: 68  AKENLQRSAVADNDNGESQVSDVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPKENGEDL 127

Query: 129 QVLRYEYGQKYDAHFDYFSDKVNIARGGHRMATVLMYLSNVEKGGETVFPSAEESQRRQA 188
           QVLRYE+GQKYDAHFDYF DKVNIARGGHR+ATVL+YLSNV KGGETVFP A+E  RR  
Sbjct: 128 QVLRYEHGQKYDAHFDYFHDKVNIARGGHRIATVLLYLSNVTKGGETVFPDAQEFSRRSL 187

Query: 189 SETNEDLSDCAKKGIAVKPRRGDALLFFSLHPNAVPDTRSLHGGCPVIEGEKWSATKWIH 248
           SE  +DLSDCAKKGIAVKP++G+ALLFF+L  +A+PD  SLHGGCPVIEGEKWSATKWIH
Sbjct: 188 SENKDDLSDCAKKGIAVKPKKGNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIH 247

Query: 249 VDSFDTILRDHTNCADEHASCERWAELGECTNNPEYMVGSPELPGYCRKSCKVC 303
           VDSFD IL    NC D + SCERWA LGEC  NPEYMVG+PE+PG CR+SCK C
Sbjct: 248 VDSFDKILTHDGNCTDVNESCERWAVLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

[Close]

BLAST of MS006055 vs. TAIR 10
Match: AT3G28480.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 366.3 bits (939), Expect = 2.3e-101
Identity = 182/313 (58.15%), Postives = 229/313 (73.16%), Query Frame = 0


Query: 4   FCSCSLLFFFSLSI-----SLLLRRASSSYAG-------SASSI-VNPAKVKQISWSPRA 63
           F + SL F F+L +     +  L R+S++  G       SASS   +P +V Q+SW+PR 
Sbjct: 6   FLAFSLCFLFTLPLISSAPNRFLTRSSNTRDGSVIKMKTSASSFGFDPTRVTQLSWTPRV 65

Query: 64  FVYEGFLTDLECDHIISLAKAELKRSAVADNLSGESKVSEVRTSSGAFIHKAKDPIISGI 123
           F+YEGFL+D ECDH I LAK +L++S VADN SGES  SEVRTSSG F+ K +D I+S +
Sbjct: 66  FLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSSGMFLSKRQDDIVSNV 125

Query: 124 EDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFSDKVNIARGGHRMATVLMYLSNVE 183
           E K+AAWTFLP+ENGE +Q+L YE GQKY+ HFDYF D+ N+  GGHR+ATVLMYLSNVE
Sbjct: 126 EAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGGHRIATVLMYLSNVE 185

Query: 184 KGGETVFPSAEESQRRQASETNED-LSDCAKKGIAVKPRRGDALLFFSLHPNAVPDTRSL 243
           KGGETVFP      + +A++  +D  ++CAK+G AVKPR+GDALLFF+LHPNA  D+ SL
Sbjct: 186 KGGETVFP----MWKGKATQLKDDSWTECAKQGYAVKPRKGDALLFFNLHPNATTDSNSL 245

Query: 244 HGGCPVIEGEKWSATKWIHVDSFDTILRDHTNCADEHASCERWAELGECTNNPEYMVGSP 303
           HG CPV+EGEKWSAT+WIHV SF+      + C DE+ SCE+WA+ GEC  NP YMVGS 
Sbjct: 246 HGSCPVVEGEKWSATRWIHVKSFERAFNKQSGCMDENVSCEKWAKAGECQKNPTYMVGSD 305

[Close]

BLAST of MS006055 vs. TAIR 10
Match: AT3G28480.2 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 344.4 bits (882), Expect = 9.5e-95
Identity = 178/321 (55.45%), Postives = 224/321 (69.78%), Query Frame = 0


Query: 4   FCSCSLLFFFSLSI-----SLLLRRASSSYAG-------SASSI-VNPAKVKQISWSPRA 63
           F + SL F F+L +     +  L R+S++  G       SASS   +P +V Q+SW+PR 
Sbjct: 6   FLAFSLCFLFTLPLISSAPNRFLTRSSNTRDGSVIKMKTSASSFGFDPTRVTQLSWTPRV 65

Query: 64  FVYEGFLTDLECDHIISLAKAELKRSAVADNLSGES-----KVSEVRTSSGAFIHKAK-- 123
           F+YEGFL+D ECDH I LAK +L++S VADN SGES      VS VR SS    +     
Sbjct: 66  FLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEDSVSVVRQSSSFIANMDSLE 125

Query: 124 -DPIISGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFSDKVNIARGGHRMATV 183
            D I+S +E K+AAWTFLP+ENGE +Q+L YE GQKY+ HFDYF D+ N+  GGHR+ATV
Sbjct: 126 IDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGGHRIATV 185

Query: 184 LMYLSNVEKGGETVFPSAEESQRRQASETNED-LSDCAKKGIAVKPRRGDALLFFSLHPN 243
           LMYLSNVEKGGETVFP      + +A++  +D  ++CAK+G AVKPR+GDALLFF+LHPN
Sbjct: 186 LMYLSNVEKGGETVFP----MWKGKATQLKDDSWTECAKQGYAVKPRKGDALLFFNLHPN 245

Query: 244 AVPDTRSLHGGCPVIEGEKWSATKWIHVDSFDTILRDHTNCADEHASCERWAELGECTNN 303
           A  D+ SLHG CPV+EGEKWSAT+WIHV SF+      + C DE+ SCE+WA+ GEC  N
Sbjct: 246 ATTDSNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQSGCMDENVSCEKWAKAGECQKN 305

[Close]

BLAST of MS006055 vs. TAIR 10
Match: AT3G28490.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 342.0 bits (876), Expect = 4.7e-94
Identity = 171/293 (58.36%), Postives = 212/293 (72.35%), Query Frame = 0


Query: 11  FFFSLSISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHIISLAK 70
           +F + S+SLLL     S   S S  V+P ++ Q+SW+PRAF+Y+GFL+D ECDH+I LAK
Sbjct: 5   YFLAFSLSLLL---IFSQISSFSFSVDPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAK 64

Query: 71  AELKRS-AVADNLSGESKVSEVRTSSGAFIHKAKDPIISGIEDKIAAWTFLPKENGEDIQ 130
            +L++S  VAD  SGES+ SEVRTSSG F+ K +D I++ +E K+AAWTFLP+ENGE +Q
Sbjct: 65  GKLEKSMVVADVDSGESEDSEVRTSSGMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQ 124

Query: 131 VLRYEYGQKYDAHFDYFSDKVNIARGGHRMATVLMYLSNVEKGGETVFPSAEESQRRQAS 190
           +L YE GQKYD HFDYF DK  +  GGHR+ATVLMYLSNV KGGETVFP+    + +   
Sbjct: 125 ILHYENGQKYDPHFDYFYDKKALELGGHRIATVLMYLSNVTKGGETVFPN---WKGKTPQ 184

Query: 191 ETNEDLSDCAKKGIAVKPRRGDALLFFSLHPNAVPDTRSLHGGCPVIEGEKWSATKWIHV 250
             ++  S CAK+G AVKPR+GDALLFF+LH N   D  SLHG CPVIEGEKWSAT+WIHV
Sbjct: 185 LKDDSWSKCAKQGYAVKPRKGDALLFFNLHLNGTTDPNSLHGSCPVIEGEKWSATRWIHV 244

Query: 251 DSFDTILRDHTNCADEHASCERWAELGECTNNPEYMVGSPELPGYCRKSCKVC 303
            SF    +    C D+H SC+ WA+ GEC  NP YMVGS    G+CRKSCK C
Sbjct: 245 RSFG---KKKLVCVDDHESCQEWADAGECEKNPMYMVGSETSLGFCRKSCKAC 288

The following BLAST results are available for this feature:

BLAST of MS006055 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022137761.1	9.1e-172	99.67	probable prolyl 4-hydroxylase 4 [Momordica charantia]	[more]
XP_022973641.1	1.4e-164	94.04	probable prolyl 4-hydroxylase 4 [Cucurbita maxima]	[more]
KAG6597483.1	3.1e-164	93.71	putative prolyl 4-hydroxylase 4, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022954026.1	7.0e-164	93.38	probable prolyl 4-hydroxylase 4 [Cucurbita moschata]	[more]
XP_023539189.1	9.1e-164	93.38	probable prolyl 4-hydroxylase 4 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS006055 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LAN3	2.0e-129	76.53	Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=...	[more]
F4JAU3	1.3e-125	73.47	Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1	[more]
Q8L970	3.3e-100	58.15	Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=...	[more]
F4J0A8	6.7e-93	58.36	Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=...	[more]
Q9LN20	2.0e-65	55.98	Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=...	[more]

BLAST of MS006055 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C7M6	4.4e-172	99.67	Procollagen-proline 4-dioxygenase OS=Momordica charantia OX=3673 GN=LOC111009125...	[more]
A0A6J1I971	6.8e-165	94.04	Procollagen-proline 4-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111472232 PE...	[more]
A0A6J1GPQ8	3.4e-164	93.38	Procollagen-proline 4-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111456381 ...	[more]
A0A1S3AWU7	1.0e-160	92.05	Procollagen-proline 4-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103483779 PE=3 S...	[more]
A0A0A0L5Q6	4.7e-158	90.40	Procollagen-proline 4-dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_3G150820 PE=...	[more]

BLAST of MS006055 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G18900.1	1.4e-130	76.53	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G06300.1	9.5e-127	73.47	P4H isoform 2	[more]
AT3G28480.1	2.3e-101	58.15	Oxoglutarate/iron-dependent oxygenase	[more]
AT3G28480.2	9.5e-95	55.45	Oxoglutarate/iron-dependent oxygenase	[more]
AT3G28490.1	4.7e-94	58.36	Oxoglutarate/iron-dependent oxygenase	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003582	ShKT domain	SMART	SM00254	ShkT_1	coord: 261..302 e-value: 0.002 score: 27.4
IPR003582	ShKT domain	PFAM	PF01549	ShK	coord: 261..302 e-value: 7.3E-5 score: 23.2
IPR003582	ShKT domain	PROSITE	PS51670	SHKT	coord: 262..302 score: 8.506279
IPR006620	Prolyl 4-hydroxylase, alpha subunit	SMART	SM00702	p4hc	coord: 48..248 e-value: 1.2E-61 score: 220.7
None	No IPR available	GENE3D	1.10.10.1940		coord: 255..302 e-value: 4.8E-5 score: 25.3
None	No IPR available	GENE3D	2.60.120.620	q2cbj1_9rhob like domain	coord: 40..249 e-value: 1.0E-76 score: 259.3
None	No IPR available	PANTHER	PTHR10869:SF175	PROLYL 4-HYDROXYLASE SUBUNIT ALPHA-LIKE PROTEIN	coord: 11..302
IPR044862	Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domain	PFAM	PF13640	2OG-FeII_Oxy_3	coord: 128..248 e-value: 3.7E-21 score: 75.8
IPR045054	Prolyl 4-hydroxylase	PANTHER	PTHR10869	PROLYL 4-HYDROXYLASE ALPHA SUBUNIT	coord: 11..302
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 124..249 score: 12.526845

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS006055.1	MS006055.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0031418	L-ascorbic acid binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen