MS006013 (gene) Bitter gourd (TR) v1

Overview
NameMS006013
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionProtein-serine/threonine phosphatase
Locationscaffold254: 3143135 .. 3147974 (-)
RNA-Seq ExpressionMS006013
SyntenyMS006013
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGAAGAAGAAGCATAAGCGGCCGGAATCAGAATCGGAGGCACTTGACAGAGGCGACTCCGAAGCTCAGCTCGTCAATGGATGTTCTCCTGAGAAGAAAAAGAGGAGGAAGAAGAAGAAACTTGAGAATGAAGAAATTGAGGGGGAGAAGAGGGAACCGGTTTCAAAACCTACAGTGAGCATTGCGGTATCCGGTTCCATCATTGACAACGCTCAATCCCTTGAGCTTGCCACCAGAGTACGCATTTTTTTCTTTTATAGGCGAGTGTTTTTTCGAGTTCCTGCCACTCAGAGGATGATATTTTGTTATGCCTTCTCTTTGAGATACTTGATTTGTTCTCTGGCGATGTCCCAACTGAGTTATGTGATCTTCCCTTTTTCCTTATGTTTCATGATTTTTTTTTCTCAGTTGGCTGGTCAAATTGCTCGTGCGGCAACCATATTCCGAATTGACGAGGTCTCCGTCTTTCTCTCTTTATTTTTTACATTTTGAAAATGCTGGTCCGATGTTAAAGAAACGTTGCTTCTTATTACATTTGATAAAAATACACTTTAGGAGGTAAATTCCAATGTTATAGGACATCCTTGGAAATGCTCAAGTCAGGAAGATAATTTTTGCGTGTATCAGGTGGTGGTATTTGACAGTGGAAGGAACTCAGTGACTGGCTCGGATATTGTGGCAGCGAACAATTCTGACGAGGATGAAAGCGGCGCTGCTTTTCTTATAAGGATCTTGAAGTATCTTGAGACACCACAATACTTGAGAAAAGCTCTTTTCCCAAAGCAAAACAATTTGAGATTTGTGGTCCAACCTTAAATTGCTTGGTTGCTTTAATCAATTAAGTAACATTTCCTGTTTTTTTCCTTCTAATCTTCCGTCTATCGTGAATTTGGTTATAGGGGATGTTGCCCCCGCTTGATGCTCCCCACCATTTGCGCAAGCATGAATGGGGTCCCTATCGAGAAGGTAAATAGGTTCTGTGTGTGAGGAGTTTATTGTTGTCTCTTCTGTTTTGAAATTCACTAGAAAACTCGCCACAAAGATTCTCTAGATTGTTCAATTTCGTACTCAAGCATTGTGTGGGTGTGGGAGGGGGTAACAAGGCACTTTCCATTGATATCAGTACACTAAGCATACAAAAATTATAGCTTTAGTTGTGTTAGACCCAAAGATCTTTGTTTTCCTTTTGAACTTTGGGCCATACTACAGTTCCTGGAGATGCATCATCACCTCGCACCAAAACACCAACGATATTGAAAAGTTCTAAAAATAGCAAACTGATAGAAAAGGGTACTGGAAGAATAAGTGAAGCTGGCTTCCGTCATTTGACCTGCACATAACGCAGATGCTTGTGTTTAAAAAAAGACCAGGAACATCTTAGTGGCAATTTTTTAGTCGATTCAATCCTGCGTCCAGATGAAGAACTTCACTTTCTCCAGCCGAATCAGCCCCTTACCTTACAGATAGCTTTGTACATATCCTCTTTCATGACTTATTTAGTGGCAGCATGATTAAGAAATGGTGATTTGCACTCCCACCAACTCTGCTGAGGAATGGAGAAGCCAAATTCTAGTGTCTACTCGGTTTCTTGGTTTCCTGCCATTAATCATTTCCAGCAACCTTGAGAATTTGTCCATCTCTTTCTCCTACAGATTTCTTCTACTCAAACTTCATTTACTTTTGCTAGTATTTATTTTCCTAAGGTTCCCAATGCCCAAGCTTCATTTCTTCGTTGGTGCTCTAGTTGACTAGATGCCTAAGCTTACTTTTCTCCTAACGTTCTTTCCTTTTTCCCAATGCCCAAGCTTTTTTTTTTTTTTTTTTCCTTTTTTAGAGTCTATAAGACATTGCTAGTAACTTGTTCATCTTCGGGCAGAAAATTTCTTTAAAGATTTTGCCATAGTTTGTGAGAAGTTTTCCGCTCATCATTGATCACTTTTACATCTGTAGCCTGAGAAATCCAAGTTTATTCTATATATTTCAAGAGGCTCCTAAAAGCTGAGTTTAGGGCTAGGGCTAGGACTATATATATTTATATATGTACCTGTCTGGCTATATTGTTTGATGACTTCTCATTCATTATGTTGGTATCCTTGGCTACTGCATTTCTTCTTGTTCACATTACATTTTGGACCTCGTTTTGTCATGTTAAACTTAGGTGTTACCCTCACACAGAGAGAAAGAGCTCCGGATGCTAGAGGAACGCCAGTTGATGTTGGTCTGAGTAAGGTAAAGCTATTTTTTATTATGGCTTCCTAGTTCTATAACTACATGTATCGATAATGAAAACATTATTTTTTTTGGATAAGAAACGATTCATTTAAAAGGAGACAAAAGAACAAATATTTTGGATAATGAAAACAAATTTTCTCCTACATTAAGCGTGAGTGGTGCTAACATGTTGAGTTAGATGGATTGGAAGGTTTAAAGTATGCTAATTCGATTTCTTTGTAGCATTTTTAAGTTCAATGCTCCCATCGACCATATATTACTTAAGTAGGTGTTTTTCTTTGGGTTAATAATTGCATTTAAATTATGCAGAATGTGGTAGTTGATGAAGTACTTGAACCTGGAATTAGAGTAACAGTAGCTATGGGAACTGATCGCAATTTACTTGCTGGTAATATCTGATACCTTTTTTAGTACATATTTAATAAAATATGAAAATTGACTAATTTTTCCTCACAGCTAAGCTATAGATGCCTTGATGAGTATTGGCTTTATGCTTGTACTTTTGAGGTCTGCAATATGGTCCAATGTCTGCAAGTATTAAGACAAAACTTGTTGAAACTGAAATAGAACATAGAGACCGAAAACTTCCTATCCAAAAAAAAGAGACCGAAAACTAGAGTTTTGTAACTTTACTACTTTAGCTGCAATGCAATACATCATTAGACAATATCCCATATTTTTTATCTGAAAAAGTAAAAACCAATAAAAGAGTGACTACTGCCAAAGCTTGTATAACTTACTCCTGATATCTAGCAACAACCAGTCGTGTCTCTTAAACAAATACATAATACAGACTTCTAAAAAATGACAAACCAACTTCTATTCTGGATCCCCCACCAAACTTGACTAATCCAACAACTAGAAAGTACTGATAACAAATTTCTAAACAATTATGAAAATGTTTCCAACAATCTTTTCTTTATGCTGATGTTCGACATTCTAACAAGGGTTGACAGTTCTTTTCTGCAATTCTTGAAAGGGTATCAAACCTATTTCAGTTGATTTGATAATATCCTTTGTGCACTTAAAACTAGATGATGGTATATTTCTTCTCCATTGAATGATTGTTACAACATGAGATTCTAACTTCTGGACCCTGTATTAGAGGAGAAATCTTGTCATATATTTATGCATGAATTTGACTTCAAATGGTTGAGGTAGGTAATATTTCAGCTATTGTCTGCCACAGTCATAGTTCGGCTCCCACTTTCATGGGCATTTAAGCCAGTATATAACCTTGTGTAGCCACAACACCATGTAATAATAGAAATGAATTTATGATCATTTTAATGCTCAACTTACGTGTATGTGTCATGCCAATGTGATTATGTCGGAAATGGAAACTAAGATTTGCCGATGGAATTCAAGATCTTAAATTTGATTTCTCCAGATCTACCCCGCCAGGTTGTCTCATCCTCAAGGCCTGTGGAAGAAGGACTTTATTGGGGTTATAGAGTGCGATATGCTTCTTGTCTGAGTGGTGTATTTAAGGAGAGTTCATACAAGGTATCTGATTATACCTCTCGATAAATCTTTTTAAATTTAATATCATATTCTTAACATTTTGTTGTTTTATTTCAAGGGTGGTTATGATCACGTGATTGGCACCTCAGAGCATGGAATGGTCGCTAAGTCCTCAGAACTAAAACTACCCTCTTTCAGGTATTTTACTTTAGCCACCCCCATGTGACCTGGATAGTTTGTTTCTAGCTGATACCTTCAGCATGTCATTTCTAGTTGATTTCTCGGTACCTTTATTCATGGCTGAAATTATCATCTGTAGTATCATTTGAACTATATTGGCAAGGTTGAACTCTGCCAACTCTGGAGAATTTTTTCTACCCCCTTCACGACATCCAACAAACTTATAAATTACAATTGTGTGCCTCTCTATATGCTTGCCTCCATAACATCATTGTTTGTAATGGAAAAATGTGTCCGAGAGGGGCTTTTAAGGAAGCAACAGCGTTATAGGCTTCTTGTCAATTAAATGTATAATGAGCCTCTGTCTTGTGCACCTTATCATAGTTCAAAAGGCAGTGTAAAGAGGTTCCATTTTTTTAACTAATACACATTTTCATACATGGGTAATTCTCTATCCGAGCCATTCTAAGCTGCAACTCTTTGGGCGATTGTTCTAATTGACAGGCATCTTTTCTGGTCCCGCTTTCTTTTTTCCATCATTAGTTGACTGATTGCTTAAATTTGCAGGCATCTTTTAATTGCCTTTGGTGGACTTGCCGGCTTGGAGGAGAGCATCGAGGAAGATAACAGCTTAAAGGTGTCCATGTTTAAAATGGTGTTCTTTGTTTGGGTTACTCCAGTCTCCAGTTCAGAACTGACTAAATTCTTGCCATTGTTTTGATCTGTCAGGGTAAAGATCCTCGCGAGATCTTCTCTTCATACTTAAATACATGCCCGCTGCAAGGAAGTCGAACAATTCGAACAGAGGTACTCCCCCTATTCCCATCTCCTCTGTTTTCTTTTTCTTCCCTACAAATTTACTGATGCATACATGGGTGGGATTGGTGATTAGGAAGCAATATTCATCTCTCTCCAGTATTTCCAAGAACCGATAAGCCGGGCGATGCAGGTGGCAGCAGAT

mRNA sequence

ATGGGGAAGAAGAAGCATAAGCGGCCGGAATCAGAATCGGAGGCACTTGACAGAGGCGACTCCGAAGCTCAGCTCGTCAATGGATGTTCTCCTGAGAAGAAAAAGAGGAGGAAGAAGAAGAAACTTGAGAATGAAGAAATTGAGGGGGAGAAGAGGGAACCGGTTTCAAAACCTACAGTGAGCATTGCGGTATCCGGTTCCATCATTGACAACGCTCAATCCCTTGAGCTTGCCACCAGATTGGCTGGTCAAATTGCTCGTGCGGCAACCATATTCCGAATTGACGAGGTGGTGGTATTTGACAGTGGAAGGAACTCAGTGACTGGCTCGGATATTGTGGCAGCGAACAATTCTGACGAGGATGAAAGCGGCGCTGCTTTTCTTATAAGGATCTTGAAGTATCTTGAGACACCACAATACTTGAGAAAAGCTCTTTTCCCAAAGCAAAACAATTTGAGATTTGTGGGGATGTTGCCCCCGCTTGATGCTCCCCACCATTTGCGCAAGCATGAATGGGGTCCCTATCGAGAAGGTGTTACCCTCACACAGAGAGAAAGAGCTCCGGATGCTAGAGGAACGCCAGTTGATGTTGGTCTGAGTAAGAATGTGGTAGTTGATGAAGTACTTGAACCTGGAATTAGAGTAACAGTAGCTATGGGAACTGATCGCAATTTACTTGCTGATCTACCCCGCCAGGTTGTCTCATCCTCAAGGCCTGTGGAAGAAGGACTTTATTGGGGTTATAGAGTGCGATATGCTTCTTGTCTGAGTGGTGTATTTAAGGAGAGTTCATACAAGGGTGGTTATGATCACGTGATTGGCACCTCAGAGCATGGAATGGTCGCTAAGTCCTCAGAACTAAAACTACCCTCTTTCAGGCATCTTTTAATTGCCTTTGGTGGACTTGCCGGCTTGGAGGAGAGCATCGAGGAAGATAACAGCTTAAAGGGTAAAGATCCTCGCGAGATCTTCTCTTCATACTTAAATACATGCCCGCTGCAAGGAAGTCGAACAATTCGAACAGAGGAAGCAATATTCATCTCTCTCCAGTATTTCCAAGAACCGATAAGCCGGGCGATGCAGGTGGCAGCAGAT

Coding sequence (CDS)

ATGGGGAAGAAGAAGCATAAGCGGCCGGAATCAGAATCGGAGGCACTTGACAGAGGCGACTCCGAAGCTCAGCTCGTCAATGGATGTTCTCCTGAGAAGAAAAAGAGGAGGAAGAAGAAGAAACTTGAGAATGAAGAAATTGAGGGGGAGAAGAGGGAACCGGTTTCAAAACCTACAGTGAGCATTGCGGTATCCGGTTCCATCATTGACAACGCTCAATCCCTTGAGCTTGCCACCAGATTGGCTGGTCAAATTGCTCGTGCGGCAACCATATTCCGAATTGACGAGGTGGTGGTATTTGACAGTGGAAGGAACTCAGTGACTGGCTCGGATATTGTGGCAGCGAACAATTCTGACGAGGATGAAAGCGGCGCTGCTTTTCTTATAAGGATCTTGAAGTATCTTGAGACACCACAATACTTGAGAAAAGCTCTTTTCCCAAAGCAAAACAATTTGAGATTTGTGGGGATGTTGCCCCCGCTTGATGCTCCCCACCATTTGCGCAAGCATGAATGGGGTCCCTATCGAGAAGGTGTTACCCTCACACAGAGAGAAAGAGCTCCGGATGCTAGAGGAACGCCAGTTGATGTTGGTCTGAGTAAGAATGTGGTAGTTGATGAAGTACTTGAACCTGGAATTAGAGTAACAGTAGCTATGGGAACTGATCGCAATTTACTTGCTGATCTACCCCGCCAGGTTGTCTCATCCTCAAGGCCTGTGGAAGAAGGACTTTATTGGGGTTATAGAGTGCGATATGCTTCTTGTCTGAGTGGTGTATTTAAGGAGAGTTCATACAAGGGTGGTTATGATCACGTGATTGGCACCTCAGAGCATGGAATGGTCGCTAAGTCCTCAGAACTAAAACTACCCTCTTTCAGGCATCTTTTAATTGCCTTTGGTGGACTTGCCGGCTTGGAGGAGAGCATCGAGGAAGATAACAGCTTAAAGGGTAAAGATCCTCGCGAGATCTTCTCTTCATACTTAAATACATGCCCGCTGCAAGGAAGTCGAACAATTCGAACAGAGGAAGCAATATTCATCTCTCTCCAGTATTTCCAAGAACCGATAAGCCGGGCGATGCAGGTGGCAGCAGAT

Protein sequence

MGKKKHKRPESESEALDRGDSEAQLVNGCSPEKKKRRKKKKLENEEIEGEKREPVSKPTVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGRNSVTGSDIVAANNSDEDESGAAFLIRILKYLETPQYLRKALFPKQNNLRFVGMLPPLDAPHHLRKHEWGPYREGVTLTQRERAPDARGTPVDVGLSKNVVVDEVLEPGIRVTVAMGTDRNLLADLPRQVVSSSRPVEEGLYWGYRVRYASCLSGVFKESSYKGGYDHVIGTSEHGMVAKSSELKLPSFRHLLIAFGGLAGLEESIEEDNSLKGKDPREIFSSYLNTCPLQGSRTIRTEEAIFISLQYFQEPISRAMQVAAD
Homology
BLAST of MS006013 vs. NCBI nr
Match: XP_022138246.1 (putative methyltransferase C9orf114 [Momordica charantia])

HSP 1 Score: 705.3 bits (1819), Expect = 2.8e-199
Identity = 361/365 (98.90%), Postives = 362/365 (99.18%), Query Frame = 0

Query: 1   MGKKKHKRPESESEALDRGDSEAQLVNGCSPEKKKRRKKKKLENEEIEGEKREPVSKPTV 60
           MGKKKHKRPESESEALDRGDSEAQLVNGCSPEKKKR+KKKKLENEEIEGEKREPVSKPTV
Sbjct: 1   MGKKKHKRPESESEALDRGDSEAQLVNGCSPEKKKRKKKKKLENEEIEGEKREPVSKPTV 60

Query: 61  SIAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGRNSVTGSDIVAANNSDE 120
           SIAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGRNSVTGSD VAANNSDE
Sbjct: 61  SIAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGRNSVTGSDTVAANNSDE 120

Query: 121 DESGAAFLIRILKYLETPQYLRKALFPKQNNLRFVGMLPPLDAPHHLRKHEWGPYREGVT 180
           DESGAAFLIRILKYLETPQYLRKALFPK NNLRFVGMLPPLDAPHHLRKHEWGPYREGVT
Sbjct: 121 DESGAAFLIRILKYLETPQYLRKALFPKHNNLRFVGMLPPLDAPHHLRKHEWGPYREGVT 180

Query: 181 LTQRERAPDARGTPVDVGLSKNVVVDEVLEPGIRVTVAMGTDRNLLADLPRQVVSSSRPV 240
           LTQRERAPDARGTPVDVGLSKNVVVDEVLEPGIRVTVAMGTDRNLLADLPRQV SSSRPV
Sbjct: 181 LTQRERAPDARGTPVDVGLSKNVVVDEVLEPGIRVTVAMGTDRNLLADLPRQVASSSRPV 240

Query: 241 EEGLYWGYRVRYASCLSGVFKESSYKGGYDHVIGTSEHGMVAKSSELKLPSFRHLLIAFG 300
           EEGLYWGYRVRYASCLSGVFKESSYKGGYDHVIGTSEHGMVAKSSELKLPSFRHLLIAFG
Sbjct: 241 EEGLYWGYRVRYASCLSGVFKESSYKGGYDHVIGTSEHGMVAKSSELKLPSFRHLLIAFG 300

Query: 301 GLAGLEESIEEDNSLKGKDPREIFSSYLNTCPLQGSRTIRTEEAIFISLQYFQEPISRAM 360
           GLAGLEESIEEDNSLKGKDPREIFSSYLNTCPLQGSRTIRTEEAIFISLQYFQEPISRAM
Sbjct: 301 GLAGLEESIEEDNSLKGKDPREIFSSYLNTCPLQGSRTIRTEEAIFISLQYFQEPISRAM 360

Query: 361 QVAAD 366
           QVAAD
Sbjct: 361 QVAAD 365

BLAST of MS006013 vs. NCBI nr
Match: XP_038879615.1 (putative methyltransferase C9orf114 [Benincasa hispida])

HSP 1 Score: 630.9 bits (1626), Expect = 6.6e-177
Identity = 325/369 (88.08%), Postives = 345/369 (93.50%), Query Frame = 0

Query: 1   MGKKKHKRPESESEALDRGDSEA----QLVNGCSPEKKKRRKKKKLENEEIEGEKREPVS 60
           MGKKKHKRPESESEALDR D EA    +  NGCSPEKKK++KKKK+EN+EI+ EKR+P+S
Sbjct: 1   MGKKKHKRPESESEALDRDDVEAKHETECSNGCSPEKKKKKKKKKVENDEIKVEKRKPIS 60

Query: 61  KPTVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGRNSVTGSDIVAAN 120
           KPTVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGR+S+TGSDI AAN
Sbjct: 61  KPTVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGRSSMTGSDIAAAN 120

Query: 121 NSDEDESGAAFLIRILKYLETPQYLRKALFPKQNNLRFVGMLPPLDAPHHLRKHEWGPYR 180
           NSDEDESGAAFLIRILKYLETPQYLRKALFPK NNLRFVGMLPPLDAPHHLRKHEWGPYR
Sbjct: 121 NSDEDESGAAFLIRILKYLETPQYLRKALFPKHNNLRFVGMLPPLDAPHHLRKHEWGPYR 180

Query: 181 EGVTLTQRERAPDARGTPVDVGLSKNVVVDEVLEPGIRVTVAMGTDRNLLADLPRQVVSS 240
           EGVTL  +ERAPDARGTPVDVGLSKNVVVDE LEPGIRVTVAMGTDRNLL+DLP QVVSS
Sbjct: 181 EGVTL--KERAPDARGTPVDVGLSKNVVVDERLEPGIRVTVAMGTDRNLLSDLPHQVVSS 240

Query: 241 SRPVEEGLYWGYRVRYASCLSGVFKESSYKGGYDHVIGTSEHGMVAKSSELKLPSFRHLL 300
           SRPVEEG YWGYRVRYAS LS VFKESSY+GGYDH+IGTSEHG+V KSSEL LPSFRHLL
Sbjct: 241 SRPVEEGFYWGYRVRYASSLSAVFKESSYEGGYDHLIGTSEHGIVVKSSELTLPSFRHLL 300

Query: 301 IAFGGLAGLEESIEEDNSLKGKDPREIFSSYLNTCPLQGSRTIRTEEAIFISLQYFQEPI 360
           IAFGGLAGLEESIEEDN+ K K+  EIFSSYLNTCPLQGSRTIRTEEAIFISLQYFQEPI
Sbjct: 301 IAFGGLAGLEESIEEDNNFKSKNAHEIFSSYLNTCPLQGSRTIRTEEAIFISLQYFQEPI 360

Query: 361 SRAMQVAAD 366
           S+A+Q+AAD
Sbjct: 361 SKALQIAAD 367

BLAST of MS006013 vs. NCBI nr
Match: XP_008438700.1 (PREDICTED: putative methyltransferase C9orf114 [Cucumis melo] >KAA0049379.1 putative methyltransferase C9orf114 [Cucumis melo var. makuwa] >TYK17180.1 putative methyltransferase C9orf114 [Cucumis melo var. makuwa])

HSP 1 Score: 622.5 bits (1604), Expect = 2.4e-174
Identity = 319/368 (86.68%), Postives = 341/368 (92.66%), Query Frame = 0

Query: 1   MGKKKHKRPESESEALDRGDSEA----QLVNGCSPEKKKRRKKKKLENEEIEGEKREPVS 60
           MGKKKHKRPE ESEALDR D EA    +L+NGCSPEKKK++KKKK+EN  IE EKR+P+S
Sbjct: 1   MGKKKHKRPEPESEALDRDDFEANRETELINGCSPEKKKKKKKKKVENGSIEAEKRKPIS 60

Query: 61  KPTVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGRNSVTGSDIVAAN 120
           KPTVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGR+S TGS+I AAN
Sbjct: 61  KPTVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGRSSTTGSEIAAAN 120

Query: 121 NSDEDESGAAFLIRILKYLETPQYLRKALFPKQNNLRFVGMLPPLDAPHHLRKHEWGPYR 180
           NSDEDESGAAFLIRILKYLETPQYLRKALFPK NNLRFVGMLPPLDAPHHLRKHEWGPYR
Sbjct: 121 NSDEDESGAAFLIRILKYLETPQYLRKALFPKHNNLRFVGMLPPLDAPHHLRKHEWGPYR 180

Query: 181 EGVTLTQRERAPDARGTPVDVGLSKNVVVDEVLEPGIRVTVAMGTDRNLLADLPRQVVSS 240
           EGVTL  +ERAPDA+GT VDVGLSKNVVVDE+LEPG RVTVAMGTDRNL +DLPRQVVSS
Sbjct: 181 EGVTL--KERAPDAKGTSVDVGLSKNVVVDEILEPGTRVTVAMGTDRNLFSDLPRQVVSS 240

Query: 241 SRPVEEGLYWGYRVRYASCLSGVFKESSYKGGYDHVIGTSEHGMVAKSSELKLPSFRHLL 300
           S+PVEEGLYWGYRVRYAS LS VFKESSY+GGYDH+IGTSEHGMV KSSEL LP FRHLL
Sbjct: 241 SKPVEEGLYWGYRVRYASSLSAVFKESSYEGGYDHLIGTSEHGMVIKSSELTLPCFRHLL 300

Query: 301 IAFGGLAGLEESIEEDNSLKGKDPREIFSSYLNTCPLQGSRTIRTEEAIFISLQYFQEPI 360
           IAFGGLAGLEESIEEDN+ K K+  EIFSSYLNTCPLQGSRTIRTEEAIFISLQYFQEPI
Sbjct: 301 IAFGGLAGLEESIEEDNNFKSKNAHEIFSSYLNTCPLQGSRTIRTEEAIFISLQYFQEPI 360

Query: 361 SRAMQVAA 365
           ++A+Q+AA
Sbjct: 361 NKAIQIAA 366

BLAST of MS006013 vs. NCBI nr
Match: XP_004134144.2 (putative methyltransferase C9orf114 [Cucumis sativus] >KGN57006.1 hypothetical protein Csa_010948 [Cucumis sativus])

HSP 1 Score: 622.1 bits (1603), Expect = 3.1e-174
Identity = 318/368 (86.41%), Postives = 341/368 (92.66%), Query Frame = 0

Query: 1   MGKKKHKRPESESEALDRGDSEA----QLVNGCSPEKKKRRKKKKLENEEIEGEKREPVS 60
           MGKKKHKRPE ESEALDR D EA    +L+NGCSPEKKK++KKKK+EN  IE EKR+P+S
Sbjct: 1   MGKKKHKRPEQESEALDRDDFEANHETELINGCSPEKKKKKKKKKVENGSIEAEKRKPIS 60

Query: 61  KPTVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGRNSVTGSDIVAAN 120
           KPTVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRI+EVVVFDSGR+S TGS++ AAN
Sbjct: 61  KPTVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRINEVVVFDSGRSSTTGSEVAAAN 120

Query: 121 NSDEDESGAAFLIRILKYLETPQYLRKALFPKQNNLRFVGMLPPLDAPHHLRKHEWGPYR 180
           NSDEDESGAAFLIRILKYLETPQYLRKALFPK NNLRFVGMLPPLDAPHHLRKHEWGPYR
Sbjct: 121 NSDEDESGAAFLIRILKYLETPQYLRKALFPKHNNLRFVGMLPPLDAPHHLRKHEWGPYR 180

Query: 181 EGVTLTQRERAPDARGTPVDVGLSKNVVVDEVLEPGIRVTVAMGTDRNLLADLPRQVVSS 240
           EGVTL  +ERAPDA+GT VDVGLSKNVVVDE+LEPG RVTVAMGTDRNL +DLPRQVVSS
Sbjct: 181 EGVTL--KERAPDAKGTSVDVGLSKNVVVDEILEPGTRVTVAMGTDRNLFSDLPRQVVSS 240

Query: 241 SRPVEEGLYWGYRVRYASCLSGVFKESSYKGGYDHVIGTSEHGMVAKSSELKLPSFRHLL 300
           S+PVEEGLYWGYRVRYAS LS VFKESSY+GGYDH+IGTSEHGMV KSSEL LP FRHLL
Sbjct: 241 SKPVEEGLYWGYRVRYASSLSAVFKESSYEGGYDHLIGTSEHGMVIKSSELTLPPFRHLL 300

Query: 301 IAFGGLAGLEESIEEDNSLKGKDPREIFSSYLNTCPLQGSRTIRTEEAIFISLQYFQEPI 360
           IAFGGLAGLEESIEEDN+ K K+  EIFSSYLNTCPLQGSRTIRTEEAIFISLQYFQEPI
Sbjct: 301 IAFGGLAGLEESIEEDNNFKSKNAHEIFSSYLNTCPLQGSRTIRTEEAIFISLQYFQEPI 360

Query: 361 SRAMQVAA 365
           ++AMQ+AA
Sbjct: 361 NKAMQIAA 366

BLAST of MS006013 vs. NCBI nr
Match: KAG6582284.1 (putative methyltransferase C9orf114-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018689.1 putative methyltransferase C9orf114-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 621.3 bits (1601), Expect = 5.2e-174
Identity = 323/374 (86.36%), Postives = 345/374 (92.25%), Query Frame = 0

Query: 1   MGKKKHKRPESESEALDRGDSEA----QLVNGCSPE-----KKKRRKKKKLENEEIEGEK 60
           MGKKKHKRPESESEALD GD EA    +L+NGCSPE     KKK+RKK+ +ENEEIEGEK
Sbjct: 1   MGKKKHKRPESESEALDGGDFEARHETELINGCSPETKKKKKKKKRKKQNVENEEIEGEK 60

Query: 61  REPVSKPTVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGRNSVTGSD 120
           R+P+SKPTVS+AVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGR+S+TGSD
Sbjct: 61  RKPISKPTVSMAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGRSSMTGSD 120

Query: 121 IVAANNSDEDESGAAFLIRILKYLETPQYLRKALFPKQNNLRFVGMLPPLDAPHHLRKHE 180
           + AANNSDEDESGAAFLIRILKYLETPQYLRKALFPK NNLRFVGMLPPLDAPHHLRKHE
Sbjct: 121 VAAANNSDEDESGAAFLIRILKYLETPQYLRKALFPKHNNLRFVGMLPPLDAPHHLRKHE 180

Query: 181 WGPYREGVTLTQRERAPDARGTPVDVGLSKNVVVDEVLEPGIRVTVAMGTDRNLLADLPR 240
           WGPYREGVTL  +ERAPDARGT VDVGLSKNVVVDE+LEPG RVTVAMGTDRNLLADLPR
Sbjct: 181 WGPYREGVTL--KERAPDARGTLVDVGLSKNVVVDEILEPGRRVTVAMGTDRNLLADLPR 240

Query: 241 QVVSSSRPVEEGLYWGYRVRYASCLSGVFKESSYKGGYDHVIGTSEHGMVAKSSELKLPS 300
           QVVSSS+PVEE LYWGYRVRYAS LS VFKESSY+GGYDH+IGTSEHG + KSSEL LPS
Sbjct: 241 QVVSSSKPVEEELYWGYRVRYASSLSAVFKESSYEGGYDHLIGTSEHGTIIKSSELILPS 300

Query: 301 FRHLLIAFGGLAGLEESIEEDNSLKGKDPREIFSSYLNTCPLQGSRTIRTEEAIFISLQY 360
           FRHLLIAFGGLAGLEESIEEDN+ K K+  EIFSSYLNTCPLQGSRTIRTEEAI ISLQY
Sbjct: 301 FRHLLIAFGGLAGLEESIEEDNNFKSKNACEIFSSYLNTCPLQGSRTIRTEEAILISLQY 360

Query: 361 FQEPISRAMQVAAD 366
           FQEPISRA+Q++AD
Sbjct: 361 FQEPISRALQISAD 372

BLAST of MS006013 vs. ExPASy Swiss-Prot
Match: Q5T280 (Putative methyltransferase C9orf114 OS=Homo sapiens OX=9606 GN=SPOUT1 PE=1 SV=3)

HSP 1 Score: 270.4 bits (690), Expect = 3.0e-71
Identity = 160/343 (46.65%), Postives = 217/343 (63.27%), Query Frame = 0

Query: 22  EAQLVNGCSPEKKKRRKKKKLENEEIEGEKREPVSKPTVSIAVSGSIIDNAQSLELATRL 81
           + +L+     ++ +  + K+LE EE   EK +     T+S+A+ GSI+DNAQS EL T L
Sbjct: 37  DLKLMKKLERQRAQEEQAKRLEEEEAAAEKEDRGRPYTLSVALPGSILDNAQSPELRTYL 96

Query: 82  AGQIARAATIFRIDEVVVFD-SGRNSVTGSDIVAANNSDEDESGAAF--LIRILKYLETP 141
           AGQIARA  IF +DE+VVFD  G+++ T    V    +   + G A   L RIL+YLE P
Sbjct: 97  AGQIARACAIFCVDEIVVFDEEGQDAKT----VEGEFTGVGKKGQACVQLARILQYLECP 156

Query: 142 QYLRKALFPKQNNLRFVGMLPPLDAPHHLRKHEWGPYREGVTLTQRERAPDARGTPVDVG 201
           QYLRKA FPK  +L+F G+L PLD+PHH+R+ E   +REG+ + +  R     G+ V+ G
Sbjct: 157 QYLRKAFFPKHQDLQFAGLLNPLDSPHHMRQDEESEFREGIVVDRPTR--PGHGSFVNCG 216

Query: 202 LSKNVVVDEVLEPGIRVTVAMGTDRNL-LADLPRQVVSSSRP-VEEGLYWGYRVRYASCL 261
           + K V +D+ LEPG+RVTV +   ++        +VVSS  P  + GLYWGY VR ASCL
Sbjct: 217 MKKEVKIDKNLEPGLRVTVRLNQQQHPDCKTYHGKVVSSQDPRTKAGLYWGYTVRLASCL 276

Query: 262 SGVFKESSYKGGYDHVIGTSEHGMVAKSSELKLPSFRHLLIAFGGLAGLEESIEEDNSLK 321
           S VF E+ ++ GYD  IGTSE G    S++  LP+FRH L+ FGGL GLE   + D +L+
Sbjct: 277 SAVFAEAPFQDGYDLTIGTSERGSDVASAQ--LPNFRHALVVFGGLQGLEAGADADPNLE 336

Query: 322 GKDPREIFSSYLNTCPLQGSRTIRTEEAIFISLQYFQEPISRA 360
             +P  +F  Y+NTCP QGSRTIRTEEAI ISL   Q  + +A
Sbjct: 337 VAEPSVLFDLYVNTCPGQGSRTIRTEEAILISLAALQPGLIQA 371

BLAST of MS006013 vs. ExPASy Swiss-Prot
Match: Q3UHX9 (Putative methyltransferase C9orf114 homolog OS=Mus musculus OX=10090 GN=Spout1 PE=1 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 1.3e-69
Identity = 158/335 (47.16%), Postives = 209/335 (62.39%), Query Frame = 0

Query: 32  EKKKRRKKKKLENEEIEGEKREPVSKP-TVSIAVSGSIIDNAQSLELATRLAGQIARAAT 91
           ++ +  + K+ E EE    +R    +P T+S+A+ GSI+DNAQS EL T LAGQIARA T
Sbjct: 47  QRAQEEEAKRQEEEEEAAAQRSNQGRPYTLSVALPGSILDNAQSPELRTYLAGQIARACT 106

Query: 92  IFRIDEVVVFDSGRNSVTGSDIVAANNSDEDESGAAFLIRILKYLETPQYLRKALFPKQN 151
           IF +DE+VVFD      T S         +       L RIL+YLE PQYLRKA FPK  
Sbjct: 107 IFCVDEIVVFDE-EGQDTKSVEGEFRGVGKKGQACVQLARILQYLECPQYLRKAFFPKHQ 166

Query: 152 NLRFVGMLPPLDAPHHLRKHEWGPYREGVTLTQRERAPDARGTPVDVGLSKNVVVDEVLE 211
           +L+F G+L PLD+PHH+R+ E   +REGV + +  +A    G+ V+ G+ K V +D+ L+
Sbjct: 167 DLQFAGILNPLDSPHHMRQDEESEFREGVVVDRPTKA--GHGSLVNCGMKKEVKIDKKLD 226

Query: 212 PGIRVTVAMGTDRNLLADLPR------QVVSSSRP-VEEGLYWGYRVRYASCLSGVFKES 271
           PG+RVTV +   +     LP        VVSS  P  + GLYWGY VR ASCLS VF E+
Sbjct: 227 PGLRVTVRLNQQQ-----LPECKTYKGTVVSSQDPRTKAGLYWGYTVRLASCLSAVFAEA 286

Query: 272 SYKGGYDHVIGTSEHGMVAKSSELKLPSFRHLLIAFGGLAGLEESIEEDNSLKGKDPREI 331
            ++ GYD  IGTSE G    S++  LPSFRH L+ FGGL GLE +++ D +L+  DP  +
Sbjct: 287 PFQDGYDLTIGTSERGSDVASAQ--LPSFRHALVVFGGLQGLEAAVDADPNLEVADPSVL 346

Query: 332 FSSYLNTCPLQGSRTIRTEEAIFISLQYFQEPISR 359
           F  Y+NTC  QGSRTIRTEEAI ISL   Q  +++
Sbjct: 347 FDFYVNTCLSQGSRTIRTEEAILISLAALQPGLTQ 371

BLAST of MS006013 vs. ExPASy Swiss-Prot
Match: Q10950 (Putative methyltransferase B0361.6 OS=Caenorhabditis elegans OX=6239 GN=B0361.6 PE=3 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 3.8e-42
Identity = 131/379 (34.56%), Postives = 197/379 (51.98%), Query Frame = 0

Query: 9   PESESEALDRGDSEAQLVNGCSPEK---KKRRKKKKLENE-----EIEGEKREPVSKP-- 68
           P+ +    ++ D   +L N    E+   +K + +KK +NE     E E  K+  + K   
Sbjct: 3   PDQKKTFKEKNDIRKKLFNSTKEERLDWRKMKDEKKRKNEEKIIKEAEEAKKAKIEKVDH 62

Query: 69  ----TVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSG----------- 128
               T+SIAV G  ++NAQS EL T +AGQIARAAT++R+DE++++D             
Sbjct: 63  TPPFTISIAVPGQFLNNAQSAELRTYMAGQIARAATLYRVDEIIIYDESCRMTDEAVNAY 122

Query: 129 RNSVTGSDIVAANNSDEDESGAAFLIRILKYLETPQYLRKALFPKQNNLRFVGMLPPLDA 188
            N     +++ A  + E   G  +L +IL+YLE PQYLRK LFP Q  L+  G+L PLDA
Sbjct: 123 YNGTWQGNLIPAETNYE---GCFYLAKILEYLECPQYLRKDLFPIQKPLKNAGLLNPLDA 182

Query: 189 PHHLRKHEWG-PYREGVTLTQRERAPDARGTPVDVGLSKNVVVDE---VLEPGIRVTVAM 248
            HHL+  E    +REGV L  ++R+ D RG   ++GL K   +D     L P  RVTV +
Sbjct: 183 QHHLKYDEKTLRFREGVVL--KKRSKDGRGPICNIGLEKEFEIDSDAVQLPPYTRVTVEI 242

Query: 249 GTDRNLL--ADLPRQVVSSSRPV--EEGLYWGYRVRYASCLSGVFKESSYKGGYDHVIGT 308
              +NL     L R  ++S   V  E GLYWGY VR  + L  V +       +D V G 
Sbjct: 243 ---KNLTEQCKLYRGSITSGATVTRETGLYWGYSVRLMTGLQKVLQAKK----FDIVAGV 302

Query: 309 SEHGMVAKSSELKLPSFRHLLIAFGGLAGLEESIEEDNSLKGKDPREIFSSYLNTCPL-Q 354
           S  G +A   ++ + +   +L+ FGG+AG++ ++E +   + +   + F   + T  L  
Sbjct: 303 SPRGKLASQMDVCILNKPKILLVFGGVAGVDAAVESEELAEWRRAEDAFDVLIRTTSLSN 362

BLAST of MS006013 vs. ExPASy Swiss-Prot
Match: Q06847 (Uncharacterized protein VNG_1688C OS=Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) OX=64091 GN=VNG_1688C PE=4 SV=2)

HSP 1 Score: 90.5 bits (223), Expect = 4.2e-17
Identity = 88/309 (28.48%), Postives = 127/309 (41.10%), Query Frame = 0

Query: 59  TVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGRNSVTGSDIVAANNS 118
           T S+ V  S++  A+    ATR  G +ARAA +FRID VVVF               +  
Sbjct: 2   TRSVLVPSSLVREAEDKREATRKLGYVARAAAVFRIDRVVVF--------------PDED 61

Query: 119 DEDESGAAFLIRILKYLETPQYLRKALFPKQNNLRFVGMLPPLDAPHHLRKHEW------ 178
            E + G  F+  +L+Y  TP YLRK  F  ++ L + G+LPP      LR   W      
Sbjct: 62  GERQWGGGFVETVLRYAATPPYLRKEAFDTRDELAYAGVLPP------LRLSSWTGSDSS 121

Query: 179 --GPYREGVTLTQ-----RERAPDARGTPVDVGLSKNVVVDEVLEPGIRVTVAMGTDRNL 238
             G  R+G+ +TQ     R R       P+ +     + V E    G RVT+ + + R +
Sbjct: 122 GSGSLRQGI-VTQVGSEGRVRVNCGMQHPISLHEPPGMAVSE----GERVTIRVSSRRPV 181

Query: 239 LADLPRQVVSSSRPVEEGL-----YWGYRVRYASCLSGVFKESSYKGGYDHVIGTSEHGM 298
            A L    +        GL          VR A+   G     +  GGY   I      +
Sbjct: 182 RAKLVDDPLPGFSVERTGLGDALDRSDAGVRIATSRHGEPLSVASLGGYRERIARDGVTV 241

Query: 299 VAKSSELKLPSFRHLLIAFGGLAGLEESIEEDNSLKGKDPREIFSSYLNTCPLQGSRTIR 350
              + E  LP           L    +++ E  +    D    F ++LNT P QGS  +R
Sbjct: 242 AFGAPERGLPPM---------LGVSADAVNESVTDSSADAPARFDAWLNTIPDQGSEVVR 276

BLAST of MS006013 vs. ExPASy Swiss-Prot
Match: P20571 (Uncharacterized protein rrnAC1612 OS=Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) OX=272569 GN=rrnAC1612 PE=4 SV=2)

HSP 1 Score: 85.5 bits (210), Expect = 1.4e-15
Identity = 92/314 (29.30%), Postives = 136/314 (43.31%), Query Frame = 0

Query: 59  TVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGRNSVTGSDIVAANNS 118
           T S+ V  S+   A+    ATR  G +ARAA ++R+D + V+                  
Sbjct: 2   TTSVLVPSSLAREAEDRREATRKLGYVARAAAVYRVDRLTVY-----------------P 61

Query: 119 DEDESGA---AFLIRILKYLETPQYLRKALFPKQNNLRFVGMLPPLDAPHHL--RKHEWG 178
           D D +G     F+  +L+Y  TP +LRK ++ K++ L +VG+LPPL             G
Sbjct: 62  DPDGAGKWEDGFVETVLRYAATPPHLRKEMWGKRDELEYVGVLPPLRVRSQTGSGSEGSG 121

Query: 179 PYREGVTLTQRERAPDARGTPVDVG----LSKNVVVDEVLEPGIRVTVAMGTDRNLLADL 238
             R+G+     E   D R   V+ G    +S  V  D  +E G RVTV            
Sbjct: 122 SLRQGIV---TEVGADGR-VRVNCGMQHPISLPVPADMDVEQGERVTVR----------- 181

Query: 239 PRQVVSSSRPVEEGLY----WGYRVRYASCLSGVFKESSYKGGYDHVIGTSEHGMVAKSS 298
               VSS RPV   L      G+ V  A   + + ++ +        I +S +G    S+
Sbjct: 182 ----VSSRRPVRAKLVDAPTTGFDVVAADLDAALSRDDA-----GLTIASSRYGEPVTST 241

Query: 299 ELKLPSFRH-----LLIAFG----GLAG-LEESIEEDNSLKGKDPREIFSSYLNTCPLQG 350
            L   + R      + +AFG    GL   L+ + +     +  D  E F  +LNT P QG
Sbjct: 242 RLGQLAERRDAEGGMTVAFGAPERGLPSILDVAPDAVGGDQTSDEPEGFDLWLNTVPNQG 274

BLAST of MS006013 vs. ExPASy TrEMBL
Match: A0A6J1C971 (putative methyltransferase C9orf114 OS=Momordica charantia OX=3673 GN=LOC111009468 PE=3 SV=1)

HSP 1 Score: 705.3 bits (1819), Expect = 1.3e-199
Identity = 361/365 (98.90%), Postives = 362/365 (99.18%), Query Frame = 0

Query: 1   MGKKKHKRPESESEALDRGDSEAQLVNGCSPEKKKRRKKKKLENEEIEGEKREPVSKPTV 60
           MGKKKHKRPESESEALDRGDSEAQLVNGCSPEKKKR+KKKKLENEEIEGEKREPVSKPTV
Sbjct: 1   MGKKKHKRPESESEALDRGDSEAQLVNGCSPEKKKRKKKKKLENEEIEGEKREPVSKPTV 60

Query: 61  SIAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGRNSVTGSDIVAANNSDE 120
           SIAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGRNSVTGSD VAANNSDE
Sbjct: 61  SIAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGRNSVTGSDTVAANNSDE 120

Query: 121 DESGAAFLIRILKYLETPQYLRKALFPKQNNLRFVGMLPPLDAPHHLRKHEWGPYREGVT 180
           DESGAAFLIRILKYLETPQYLRKALFPK NNLRFVGMLPPLDAPHHLRKHEWGPYREGVT
Sbjct: 121 DESGAAFLIRILKYLETPQYLRKALFPKHNNLRFVGMLPPLDAPHHLRKHEWGPYREGVT 180

Query: 181 LTQRERAPDARGTPVDVGLSKNVVVDEVLEPGIRVTVAMGTDRNLLADLPRQVVSSSRPV 240
           LTQRERAPDARGTPVDVGLSKNVVVDEVLEPGIRVTVAMGTDRNLLADLPRQV SSSRPV
Sbjct: 181 LTQRERAPDARGTPVDVGLSKNVVVDEVLEPGIRVTVAMGTDRNLLADLPRQVASSSRPV 240

Query: 241 EEGLYWGYRVRYASCLSGVFKESSYKGGYDHVIGTSEHGMVAKSSELKLPSFRHLLIAFG 300
           EEGLYWGYRVRYASCLSGVFKESSYKGGYDHVIGTSEHGMVAKSSELKLPSFRHLLIAFG
Sbjct: 241 EEGLYWGYRVRYASCLSGVFKESSYKGGYDHVIGTSEHGMVAKSSELKLPSFRHLLIAFG 300

Query: 301 GLAGLEESIEEDNSLKGKDPREIFSSYLNTCPLQGSRTIRTEEAIFISLQYFQEPISRAM 360
           GLAGLEESIEEDNSLKGKDPREIFSSYLNTCPLQGSRTIRTEEAIFISLQYFQEPISRAM
Sbjct: 301 GLAGLEESIEEDNSLKGKDPREIFSSYLNTCPLQGSRTIRTEEAIFISLQYFQEPISRAM 360

Query: 361 QVAAD 366
           QVAAD
Sbjct: 361 QVAAD 365

BLAST of MS006013 vs. ExPASy TrEMBL
Match: A0A5D3D0N4 (Putative methyltransferase C9orf114 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00240 PE=3 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 1.1e-174
Identity = 319/368 (86.68%), Postives = 341/368 (92.66%), Query Frame = 0

Query: 1   MGKKKHKRPESESEALDRGDSEA----QLVNGCSPEKKKRRKKKKLENEEIEGEKREPVS 60
           MGKKKHKRPE ESEALDR D EA    +L+NGCSPEKKK++KKKK+EN  IE EKR+P+S
Sbjct: 1   MGKKKHKRPEPESEALDRDDFEANRETELINGCSPEKKKKKKKKKVENGSIEAEKRKPIS 60

Query: 61  KPTVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGRNSVTGSDIVAAN 120
           KPTVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGR+S TGS+I AAN
Sbjct: 61  KPTVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGRSSTTGSEIAAAN 120

Query: 121 NSDEDESGAAFLIRILKYLETPQYLRKALFPKQNNLRFVGMLPPLDAPHHLRKHEWGPYR 180
           NSDEDESGAAFLIRILKYLETPQYLRKALFPK NNLRFVGMLPPLDAPHHLRKHEWGPYR
Sbjct: 121 NSDEDESGAAFLIRILKYLETPQYLRKALFPKHNNLRFVGMLPPLDAPHHLRKHEWGPYR 180

Query: 181 EGVTLTQRERAPDARGTPVDVGLSKNVVVDEVLEPGIRVTVAMGTDRNLLADLPRQVVSS 240
           EGVTL  +ERAPDA+GT VDVGLSKNVVVDE+LEPG RVTVAMGTDRNL +DLPRQVVSS
Sbjct: 181 EGVTL--KERAPDAKGTSVDVGLSKNVVVDEILEPGTRVTVAMGTDRNLFSDLPRQVVSS 240

Query: 241 SRPVEEGLYWGYRVRYASCLSGVFKESSYKGGYDHVIGTSEHGMVAKSSELKLPSFRHLL 300
           S+PVEEGLYWGYRVRYAS LS VFKESSY+GGYDH+IGTSEHGMV KSSEL LP FRHLL
Sbjct: 241 SKPVEEGLYWGYRVRYASSLSAVFKESSYEGGYDHLIGTSEHGMVIKSSELTLPCFRHLL 300

Query: 301 IAFGGLAGLEESIEEDNSLKGKDPREIFSSYLNTCPLQGSRTIRTEEAIFISLQYFQEPI 360
           IAFGGLAGLEESIEEDN+ K K+  EIFSSYLNTCPLQGSRTIRTEEAIFISLQYFQEPI
Sbjct: 301 IAFGGLAGLEESIEEDNNFKSKNAHEIFSSYLNTCPLQGSRTIRTEEAIFISLQYFQEPI 360

Query: 361 SRAMQVAA 365
           ++A+Q+AA
Sbjct: 361 NKAIQIAA 366

BLAST of MS006013 vs. ExPASy TrEMBL
Match: A0A1S3AWP4 (putative methyltransferase C9orf114 OS=Cucumis melo OX=3656 GN=LOC103483727 PE=3 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 1.1e-174
Identity = 319/368 (86.68%), Postives = 341/368 (92.66%), Query Frame = 0

Query: 1   MGKKKHKRPESESEALDRGDSEA----QLVNGCSPEKKKRRKKKKLENEEIEGEKREPVS 60
           MGKKKHKRPE ESEALDR D EA    +L+NGCSPEKKK++KKKK+EN  IE EKR+P+S
Sbjct: 1   MGKKKHKRPEPESEALDRDDFEANRETELINGCSPEKKKKKKKKKVENGSIEAEKRKPIS 60

Query: 61  KPTVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGRNSVTGSDIVAAN 120
           KPTVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGR+S TGS+I AAN
Sbjct: 61  KPTVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGRSSTTGSEIAAAN 120

Query: 121 NSDEDESGAAFLIRILKYLETPQYLRKALFPKQNNLRFVGMLPPLDAPHHLRKHEWGPYR 180
           NSDEDESGAAFLIRILKYLETPQYLRKALFPK NNLRFVGMLPPLDAPHHLRKHEWGPYR
Sbjct: 121 NSDEDESGAAFLIRILKYLETPQYLRKALFPKHNNLRFVGMLPPLDAPHHLRKHEWGPYR 180

Query: 181 EGVTLTQRERAPDARGTPVDVGLSKNVVVDEVLEPGIRVTVAMGTDRNLLADLPRQVVSS 240
           EGVTL  +ERAPDA+GT VDVGLSKNVVVDE+LEPG RVTVAMGTDRNL +DLPRQVVSS
Sbjct: 181 EGVTL--KERAPDAKGTSVDVGLSKNVVVDEILEPGTRVTVAMGTDRNLFSDLPRQVVSS 240

Query: 241 SRPVEEGLYWGYRVRYASCLSGVFKESSYKGGYDHVIGTSEHGMVAKSSELKLPSFRHLL 300
           S+PVEEGLYWGYRVRYAS LS VFKESSY+GGYDH+IGTSEHGMV KSSEL LP FRHLL
Sbjct: 241 SKPVEEGLYWGYRVRYASSLSAVFKESSYEGGYDHLIGTSEHGMVIKSSELTLPCFRHLL 300

Query: 301 IAFGGLAGLEESIEEDNSLKGKDPREIFSSYLNTCPLQGSRTIRTEEAIFISLQYFQEPI 360
           IAFGGLAGLEESIEEDN+ K K+  EIFSSYLNTCPLQGSRTIRTEEAIFISLQYFQEPI
Sbjct: 301 IAFGGLAGLEESIEEDNNFKSKNAHEIFSSYLNTCPLQGSRTIRTEEAIFISLQYFQEPI 360

Query: 361 SRAMQVAA 365
           ++A+Q+AA
Sbjct: 361 NKAIQIAA 366

BLAST of MS006013 vs. ExPASy TrEMBL
Match: A0A0A0LAE4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G149370 PE=3 SV=1)

HSP 1 Score: 622.1 bits (1603), Expect = 1.5e-174
Identity = 318/368 (86.41%), Postives = 341/368 (92.66%), Query Frame = 0

Query: 1   MGKKKHKRPESESEALDRGDSEA----QLVNGCSPEKKKRRKKKKLENEEIEGEKREPVS 60
           MGKKKHKRPE ESEALDR D EA    +L+NGCSPEKKK++KKKK+EN  IE EKR+P+S
Sbjct: 1   MGKKKHKRPEQESEALDRDDFEANHETELINGCSPEKKKKKKKKKVENGSIEAEKRKPIS 60

Query: 61  KPTVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGRNSVTGSDIVAAN 120
           KPTVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRI+EVVVFDSGR+S TGS++ AAN
Sbjct: 61  KPTVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRINEVVVFDSGRSSTTGSEVAAAN 120

Query: 121 NSDEDESGAAFLIRILKYLETPQYLRKALFPKQNNLRFVGMLPPLDAPHHLRKHEWGPYR 180
           NSDEDESGAAFLIRILKYLETPQYLRKALFPK NNLRFVGMLPPLDAPHHLRKHEWGPYR
Sbjct: 121 NSDEDESGAAFLIRILKYLETPQYLRKALFPKHNNLRFVGMLPPLDAPHHLRKHEWGPYR 180

Query: 181 EGVTLTQRERAPDARGTPVDVGLSKNVVVDEVLEPGIRVTVAMGTDRNLLADLPRQVVSS 240
           EGVTL  +ERAPDA+GT VDVGLSKNVVVDE+LEPG RVTVAMGTDRNL +DLPRQVVSS
Sbjct: 181 EGVTL--KERAPDAKGTSVDVGLSKNVVVDEILEPGTRVTVAMGTDRNLFSDLPRQVVSS 240

Query: 241 SRPVEEGLYWGYRVRYASCLSGVFKESSYKGGYDHVIGTSEHGMVAKSSELKLPSFRHLL 300
           S+PVEEGLYWGYRVRYAS LS VFKESSY+GGYDH+IGTSEHGMV KSSEL LP FRHLL
Sbjct: 241 SKPVEEGLYWGYRVRYASSLSAVFKESSYEGGYDHLIGTSEHGMVIKSSELTLPPFRHLL 300

Query: 301 IAFGGLAGLEESIEEDNSLKGKDPREIFSSYLNTCPLQGSRTIRTEEAIFISLQYFQEPI 360
           IAFGGLAGLEESIEEDN+ K K+  EIFSSYLNTCPLQGSRTIRTEEAIFISLQYFQEPI
Sbjct: 301 IAFGGLAGLEESIEEDNNFKSKNAHEIFSSYLNTCPLQGSRTIRTEEAIFISLQYFQEPI 360

Query: 361 SRAMQVAA 365
           ++AMQ+AA
Sbjct: 361 NKAMQIAA 366

BLAST of MS006013 vs. ExPASy TrEMBL
Match: A0A6J1GWH0 (putative methyltransferase C9orf114 homolog OS=Cucurbita moschata OX=3662 GN=LOC111458056 PE=3 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 9.7e-174
Identity = 322/372 (86.56%), Postives = 343/372 (92.20%), Query Frame = 0

Query: 1   MGKKKHKRPESESEALDRGDSEA----QLVNGCSPE---KKKRRKKKKLENEEIEGEKRE 60
           MGKKKHKRPESESEALD GD EA    +L+NGCSPE   KKK+RKK+ +ENEEIEGEKR+
Sbjct: 1   MGKKKHKRPESESEALDGGDFEARHETELINGCSPETKKKKKKRKKQNVENEEIEGEKRK 60

Query: 61  PVSKPTVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGRNSVTGSDIV 120
           P+SKPTVS+AVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGR+S+TGSD+ 
Sbjct: 61  PISKPTVSMAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGRSSMTGSDVA 120

Query: 121 AANNSDEDESGAAFLIRILKYLETPQYLRKALFPKQNNLRFVGMLPPLDAPHHLRKHEWG 180
           AANNSDEDESGAAFLIRILKYLETPQYLRKALFPK NNLRFVGMLPPLDAPHHLRKHEWG
Sbjct: 121 AANNSDEDESGAAFLIRILKYLETPQYLRKALFPKHNNLRFVGMLPPLDAPHHLRKHEWG 180

Query: 181 PYREGVTLTQRERAPDARGTPVDVGLSKNVVVDEVLEPGIRVTVAMGTDRNLLADLPRQV 240
           PYREGVTL  +ERAPDARGT VDVGLSKNVVVDE+LEPG RVTVAMGTDRNLLADLPRQV
Sbjct: 181 PYREGVTL--KERAPDARGTLVDVGLSKNVVVDEILEPGRRVTVAMGTDRNLLADLPRQV 240

Query: 241 VSSSRPVEEGLYWGYRVRYASCLSGVFKESSYKGGYDHVIGTSEHGMVAKSSELKLPSFR 300
           VSSS PVEE LYWGYRVRYAS LS VF ESSY+GGYDH+IGTSEHG + KSSEL LPSFR
Sbjct: 241 VSSSNPVEEELYWGYRVRYASSLSAVFNESSYEGGYDHLIGTSEHGTIIKSSELILPSFR 300

Query: 301 HLLIAFGGLAGLEESIEEDNSLKGKDPREIFSSYLNTCPLQGSRTIRTEEAIFISLQYFQ 360
           HLLIAFGGLAGLEESIEEDN+ K K+  EIFSSYLNTCPLQGSRTIRTEEAI ISLQYFQ
Sbjct: 301 HLLIAFGGLAGLEESIEEDNNFKSKNACEIFSSYLNTCPLQGSRTIRTEEAILISLQYFQ 360

Query: 361 EPISRAMQVAAD 366
           EPISRA+Q++AD
Sbjct: 361 EPISRALQISAD 370

BLAST of MS006013 vs. TAIR 10
Match: AT5G19300.1 (CONTAINS InterPro DOMAIN/s: Nucleic acid-binding, OB-fold-like (InterPro:IPR016027), Protein of unknown function DUF171 (InterPro:IPR003750); Has 3649 Blast hits to 1964 proteins in 291 species: Archae - 113; Bacteria - 121; Metazoa - 1082; Fungi - 399; Plants - 227; Viruses - 4; Other Eukaryotes - 1703 (source: NCBI BLink). )

HSP 1 Score: 444.9 bits (1143), Expect = 6.3e-125
Identity = 246/372 (66.13%), Postives = 294/372 (79.03%), Query Frame = 0

Query: 3   KKKHKRPESESE---ALDRGDSEAQLVNGCSPEKKKRRKKKKLENEEIEGEKRE------ 62
           K+ H+  E E E   +LD GDS+ + +      KKKR+ K + E  E+  EK +      
Sbjct: 38  KRSHEDTEIEPEQKMSLD-GDSKEEKI------KKKRKNKNQEEEPELVTEKTKVQEEEK 97

Query: 63  ---PVSKPTVSIAVSGSIIDNAQSLELATRLAGQIARAATIFRIDEVVVFDSGRNSVTGS 122
                 + TVSIA++GSII N QSLELATRLAGQIARAATIFRIDE+VVFD+  +S   S
Sbjct: 98  GNVEEGRATVSIAIAGSIIHNTQSLELATRLAGQIARAATIFRIDEIVVFDNKSSSEIES 157

Query: 123 DIVAANNSDEDESGAAFLIRILKYLETPQYLRKALFPKQNNLRFVGMLPPLDAPHHLRKH 182
              A N SD +ESGA+FL+RILKYLETPQYLRK+LFPKQN+LR+VGMLPPLDAPHHLRKH
Sbjct: 158 --AATNASDSNESGASFLVRILKYLETPQYLRKSLFPKQNDLRYVGMLPPLDAPHHLRKH 217

Query: 183 EWGPYREGVTLTQRERAPDARGTPVDVGLSKNVVVDEVLEPGIRVTVAMGTDRNLLADLP 242
           EW  YREGVTL+  E+AP++ GT VDVGLSK+VVVD+VL PGIRVTVAMGTD +L  DL 
Sbjct: 218 EWEQYREGVTLS--EKAPNSEGTLVDVGLSKSVVVDQVLGPGIRVTVAMGTDHDL--DLV 277

Query: 243 RQVVSSSRPVEE-GLYWGYRVRYASCLSGVFKESSYKGGYDHVIGTSEHGMVAKSSELKL 302
           RQ+V  S+P EE G+YWGY+VRYAS LS VFKE  ++GGYD++IGTSEHG+V  SSELK+
Sbjct: 278 RQIVPPSKPREEAGMYWGYKVRYASQLSSVFKECPFEGGYDYLIGTSEHGLVISSSELKI 337

Query: 303 PSFRHLLIAFGGLAGLEESIEEDNSLKGKDPREIFSSYLNTCPLQGSRTIRTEEAIFISL 362
           P+FRHLLIAFGGLAGLEESIE+DN  KGK+ R++F+ YLNTCP QGSRTIR EEA+FISL
Sbjct: 338 PTFRHLLIAFGGLAGLEESIEDDNQYKGKNVRDVFNVYLNTCPHQGSRTIRAEEAMFISL 396

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022138246.12.8e-19998.90putative methyltransferase C9orf114 [Momordica charantia][more]
XP_038879615.16.6e-17788.08putative methyltransferase C9orf114 [Benincasa hispida][more]
XP_008438700.12.4e-17486.68PREDICTED: putative methyltransferase C9orf114 [Cucumis melo] >KAA0049379.1 puta... [more]
XP_004134144.23.1e-17486.41putative methyltransferase C9orf114 [Cucumis sativus] >KGN57006.1 hypothetical p... [more]
KAG6582284.15.2e-17486.36putative methyltransferase C9orf114-like protein, partial [Cucurbita argyrosperm... [more]
Match NameE-valueIdentityDescription
Q5T2803.0e-7146.65Putative methyltransferase C9orf114 OS=Homo sapiens OX=9606 GN=SPOUT1 PE=1 SV=3[more]
Q3UHX91.3e-6947.16Putative methyltransferase C9orf114 homolog OS=Mus musculus OX=10090 GN=Spout1 P... [more]
Q109503.8e-4234.56Putative methyltransferase B0361.6 OS=Caenorhabditis elegans OX=6239 GN=B0361.6 ... [more]
Q068474.2e-1728.48Uncharacterized protein VNG_1688C OS=Halobacterium salinarum (strain ATCC 700922... [more]
P205711.4e-1529.30Uncharacterized protein rrnAC1612 OS=Haloarcula marismortui (strain ATCC 43049 /... [more]
Match NameE-valueIdentityDescription
A0A6J1C9711.3e-19998.90putative methyltransferase C9orf114 OS=Momordica charantia OX=3673 GN=LOC1110094... [more]
A0A5D3D0N41.1e-17486.68Putative methyltransferase C9orf114 OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A1S3AWP41.1e-17486.68putative methyltransferase C9orf114 OS=Cucumis melo OX=3656 GN=LOC103483727 PE=3... [more]
A0A0A0LAE41.5e-17486.41Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G149370 PE=3 SV=1[more]
A0A6J1GWH09.7e-17486.56putative methyltransferase C9orf114 homolog OS=Cucurbita moschata OX=3662 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT5G19300.16.3e-12566.13CONTAINS InterPro DOMAIN/s: Nucleic acid-binding, OB-fold-like (InterPro:IPR0160... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D2.40.50.140coord: 170..237
e-value: 1.3E-93
score: 315.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..19
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 39..55
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..58
IPR003750Putative RNA methyltransferasePFAMPF02598Methyltrn_RNA_3coord: 59..351
e-value: 3.8E-98
score: 328.5
IPR003750Putative RNA methyltransferasePANTHERPTHR12150CLASS IV SAM-BINDING METHYLTRANSFERASE-RELATEDcoord: 4..361
IPR003750Putative RNA methyltransferaseCDDcd18086HsC9orf114-likecoord: 59..352
e-value: 3.47998E-71
score: 218.661
IPR029026tRNA (guanine-N1-)-methyltransferase, N-terminalGENE3D3.40.1280.10coord: 60..350
e-value: 1.3E-93
score: 315.5
IPR029028Alpha/beta knot methyltransferasesSUPERFAMILY75217alpha/beta knotcoord: 57..353
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 164..247

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS006013.1MS006013.1mRNA