MS005718 (gene) Bitter gourd (TR) v1

Overview


Name	MS005718
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Pectinesterase
Location	scaffold254: 1072670 .. 1075411 (+)
RNA-Seq Expression	MS005718
Synteny	MS005718

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCCTCTCCCTTCGCGGCCTTTCTTCTCCTCCTCCTAGCCGTGGCTTCCTCAATTTCGTCATTCCACGACCCAGACCGGGAGGTTCAGTTGGCCCGGACCATAGTTATGGAGGCCAGAGACTCGGTCCGAGGCTCCGTGACCGGGATAGGTGAGTTAAAACGACGCCGCTTTGAACGGCGGAATTTTGTTGAGCCCGCGCTCGGCGATTGCACCAGGCTTTACGAGGAGGCCGACGCGCGGCTGGCGGAGCTGCTGTTCGGAGGAAATTACACGGCGGAAGATGCTCGGACGTGGATCAGCGCCGCCGTGGCTAATCATCGGAGTTGTACGGACGGCCTGGAAGAGGTCGGAGCTGCGGCGGATAGGAAGAATAATTTGACTGCGTTACTCGCCGGAGCTCTGCGTTTATACGAGAAAATCGCCGCCGTGGAGGAGCGTAATGGTAACCACACGTACTTCCGACGTTGCATTCATTCGGATATTCGAAGTTATTAGTTTTCTATTTAAATATATCACTGAAAATCGTTTGTTCTTTTTTCTTTTTTGTTTCGTTTCGTATCTTTATTCGTGGTCGTCTTAATGTTCAAAATTAATTGTTTTCTATGTTTTTAGTCTACTAATAATAAATATGGTACTAAAAAAAATACCTGAAAATATTTGACTCCTGTCTTTACAAAATTGACCAACTTATTATTATTTTCAGACAATTCATTATTATTTTCTTTGAGTATTTAGGTTTGGATTTCTCTCTTTTGCTTATCAAATATTTAGGGACAAGAACCATCTAAAATCTAAAATGTGATCGAATATTTAGGGATAAAGAACAATCTAAAAAAGTAACCATCTTGCTGTTAGGTTTACTAGAATTTTCACTTTTAAAATAGAAGTTTTGCAAACATTTTGTGAAGAATTGATAAACTATCCTTCCCAAGCTCAAAATTGGCTTAAATTGATGGGTTGGTAAATTGAATTCAATATATCTCATTTAAATTGATGGGTTGGTTATATATAAATATTTTATAAAATATATTATAAAAATGGCACAACGATAAGAACTCATTTAATAATGATGTGATTTATATGTTTACTACATTGGTGATAAAATAAAGTATATTAACACTTTATCTATAGATGATGTGTCTATAGATTTATGTATTGTATAAATCATACATAAGCGTAAGTCAATTAGTTATTTAAGCACTTGTCGGTAAGATTGTGAGTTCCAATTCTCACACCTACTTGAGTGTGAGTGAGTGTGATAGATAAGTATATGTATTTGAACGAGTGTGATGGATAAGCATGTATTTTTTTACTTATTTAGTAACTAGTTTACCTTTAAAATATAAAAAATAAAATAAAATATCCTTATAAGAATTAAGTTGTCACCTAATTAGCCCAACGAGCATAGCTAAGTAATTCAAATACATATTTCTGAATAAAAAGTCATACGTTCGAACTCTCCCAATATGTTGTATCTCATCAAAAAAAAGTTGTCGCCTAATTGTATTTTTCTCACGTCTCAATGTATTTATTTAAAAGATACATATGGACTTTTGAATTCAGGATTGAAACGATGGGAGGAGCAGAAATTGACGAAAAATAGAGGAATCGACTTGGCGAAATGGAATCCGGCGACGTCGAAGGCGGACTATGTGGTGGCGATGGACGGCTCCGGCACACACAGAACCATAAACGACGCGGTGGCGGCTCTGGCGAGGAGTGGCCGGAGACGGCGGTCGGAGCGAGTGGTGATTTACGTGAAAGCCGGAGTTTACAGGGAGAACGTGGAAATCGGGATGCAATTGAAGAATGTGTTTATTGTCGGCGACGGCGCCGACAGAACCACCATCACCGGCAGCCGCAACGTGCCGGACGGCGCCACCACTTACAGCTCAGCAACATTTGGTGAGGCGAAACGTATTTATCACAAAACAATAAAATATTATTTAATAATATTGAAAATAATAATAAAATATATAAAGGTTTATGGAGGCATTTAAAATTAATGAAGCTTACAAAATTAACTTTGATGAAACTTTGGTATGAGCAGGCGTCTCCGGCGACGGATTTTTGGCAAGGGACATCACCTTCGAGAACACCGCCGGGCCGGAAAAGCACCAGGCGGTGGCGCTGCGGGTAAATTCCGACCTCGCCGTCGTCTACCGCTGCACAATCAAAGGCTACCAGGACTCCCTCTTCCTCCACTCCCTCCGCCAATTCTACCGCGACTGCCGCATATACGGCACCGTCGATTTCATCTTCGGAAACTCCGCCGCCGTCCTCCAGAACTGCGAAATCCTCGTCCGGCGGCCGATGCGCCACCAAGCCAACATGATCACCGCCCAAGGCCGCGACGATCCGGCGGAGAACACCGGAATTTCGATCCACGGCTCGCGAGTCAGGGCGGCACCGGAATTCGCCGCCGTACAGGGCGAATTCAGAACGTTCCTCGGCCGGCCGTGGAAGCGGTACTCGAGGACGGTGGTTATGGCGACGGATCTGGAGGGATTGATCGATCCGCGTGGGTGGGGAGAATGGGAAGGGGATTTTGCTCTGTCGACTCTGTTTTATGGTGAATATAAGAACACCGGCCGCGGTTCTTCCACTCGATTTAGGGTTAATTGGCCCGGATTTCACATTTTTCGCGGCGATCTGGAGGCCGGAAGGTTTACGGTCGGCGGTTTTCTCCACGGCCGCGATTGGATTCCGGTGACCGGAGTGCCCTTCCAGGCAGGAATT

mRNA sequence

ATGCCCTCTCCCTTCGCGGCCTTTCTTCTCCTCCTCCTAGCCGTGGCTTCCTCAATTTCGTCATTCCACGACCCAGACCGGGAGGTTCAGTTGGCCCGGACCATAGTTATGGAGGCCAGAGACTCGGTCCGAGGCTCCGTGACCGGGATAGGTGAGTTAAAACGACGCCGCTTTGAACGGCGGAATTTTGTTGAGCCCGCGCTCGGCGATTGCACCAGGCTTTACGAGGAGGCCGACGCGCGGCTGGCGGAGCTGCTGTTCGGAGGAAATTACACGGCGGAAGATGCTCGGACGTGGATCAGCGCCGCCGTGGCTAATCATCGGAGTTGTACGGACGGCCTGGAAGAGGTCGGAGCTGCGGCGGATAGGAAGAATAATTTGACTGCGTTACTCGCCGGAGCTCTGCGTTTATACGAGAAAATCGCCGCCGTGGAGGAGCGTAATGGATTGAAACGATGGGAGGAGCAGAAATTGACGAAAAATAGAGGAATCGACTTGGCGAAATGGAATCCGGCGACGTCGAAGGCGGACTATGTGGTGGCGATGGACGGCTCCGGCACACACAGAACCATAAACGACGCGGTGGCGGCTCTGGCGAGGAGTGGCCGGAGACGGCGGTCGGAGCGAGTGGTGATTTACGTGAAAGCCGGAGTTTACAGGGAGAACGTGGAAATCGGGATGCAATTGAAGAATGTGTTTATTGTCGGCGACGGCGCCGACAGAACCACCATCACCGGCAGCCGCAACGTGCCGGACGGCGCCACCACTTACAGCTCAGCAACATTTGCAGGCGTCTCCGGCGACGGATTTTTGGCAAGGGACATCACCTTCGAGAACACCGCCGGGCCGGAAAAGCACCAGGCGGTGGCGCTGCGGGTAAATTCCGACCTCGCCGTCGTCTACCGCTGCACAATCAAAGGCTACCAGGACTCCCTCTTCCTCCACTCCCTCCGCCAATTCTACCGCGACTGCCGCATATACGGCACCGTCGATTTCATCTTCGGAAACTCCGCCGCCGTCCTCCAGAACTGCGAAATCCTCGTCCGGCGGCCGATGCGCCACCAAGCCAACATGATCACCGCCCAAGGCCGCGACGATCCGGCGGAGAACACCGGAATTTCGATCCACGGCTCGCGAGTCAGGGCGGCACCGGAATTCGCCGCCGTACAGGGCGAATTCAGAACGTTCCTCGGCCGGCCGTGGAAGCGGTACTCGAGGACGGTGGTTATGGCGACGGATCTGGAGGGATTGATCGATCCGCGTGGGTGGGGAGAATGGGAAGGGGATTTTGCTCTGTCGACTCTGTTTTATGGTGAATATAAGAACACCGGCCGCGGTTCTTCCACTCGATTTAGGGTTAATTGGCCCGGATTTCACATTTTTCGCGGCGATCTGGAGGCCGGAAGGTTTACGGTCGGCGGTTTTCTCCACGGCCGCGATTGGATTCCGGTGACCGGAGTGCCCTTCCAGGCAGGAATT

Coding sequence (CDS)

ATGCCCTCTCCCTTCGCGGCCTTTCTTCTCCTCCTCCTAGCCGTGGCTTCCTCAATTTCGTCATTCCACGACCCAGACCGGGAGGTTCAGTTGGCCCGGACCATAGTTATGGAGGCCAGAGACTCGGTCCGAGGCTCCGTGACCGGGATAGGTGAGTTAAAACGACGCCGCTTTGAACGGCGGAATTTTGTTGAGCCCGCGCTCGGCGATTGCACCAGGCTTTACGAGGAGGCCGACGCGCGGCTGGCGGAGCTGCTGTTCGGAGGAAATTACACGGCGGAAGATGCTCGGACGTGGATCAGCGCCGCCGTGGCTAATCATCGGAGTTGTACGGACGGCCTGGAAGAGGTCGGAGCTGCGGCGGATAGGAAGAATAATTTGACTGCGTTACTCGCCGGAGCTCTGCGTTTATACGAGAAAATCGCCGCCGTGGAGGAGCGTAATGGATTGAAACGATGGGAGGAGCAGAAATTGACGAAAAATAGAGGAATCGACTTGGCGAAATGGAATCCGGCGACGTCGAAGGCGGACTATGTGGTGGCGATGGACGGCTCCGGCACACACAGAACCATAAACGACGCGGTGGCGGCTCTGGCGAGGAGTGGCCGGAGACGGCGGTCGGAGCGAGTGGTGATTTACGTGAAAGCCGGAGTTTACAGGGAGAACGTGGAAATCGGGATGCAATTGAAGAATGTGTTTATTGTCGGCGACGGCGCCGACAGAACCACCATCACCGGCAGCCGCAACGTGCCGGACGGCGCCACCACTTACAGCTCAGCAACATTTGCAGGCGTCTCCGGCGACGGATTTTTGGCAAGGGACATCACCTTCGAGAACACCGCCGGGCCGGAAAAGCACCAGGCGGTGGCGCTGCGGGTAAATTCCGACCTCGCCGTCGTCTACCGCTGCACAATCAAAGGCTACCAGGACTCCCTCTTCCTCCACTCCCTCCGCCAATTCTACCGCGACTGCCGCATATACGGCACCGTCGATTTCATCTTCGGAAACTCCGCCGCCGTCCTCCAGAACTGCGAAATCCTCGTCCGGCGGCCGATGCGCCACCAAGCCAACATGATCACCGCCCAAGGCCGCGACGATCCGGCGGAGAACACCGGAATTTCGATCCACGGCTCGCGAGTCAGGGCGGCACCGGAATTCGCCGCCGTACAGGGCGAATTCAGAACGTTCCTCGGCCGGCCGTGGAAGCGGTACTCGAGGACGGTGGTTATGGCGACGGATCTGGAGGGATTGATCGATCCGCGTGGGTGGGGAGAATGGGAAGGGGATTTTGCTCTGTCGACTCTGTTTTATGGTGAATATAAGAACACCGGCCGCGGTTCTTCCACTCGATTTAGGGTTAATTGGCCCGGATTTCACATTTTTCGCGGCGATCTGGAGGCCGGAAGGTTTACGGTCGGCGGTTTTCTCCACGGCCGCGATTGGATTCCGGTGACCGGAGTGCCCTTCCAGGCAGGAATT

Protein sequence

MPSPFAAFLLLLLAVASSISSFHDPDREVQLARTIVMEARDSVRGSVTGIGELKRRRFERRNFVEPALGDCTRLYEEADARLAELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGAAADRKNNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKADYVVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGDGADRTTITGSRNVPDGATTYSSATFAGVSGDGFLARDITFENTAGPEKHQAVALRVNSDLAVVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEILVRRPMRHQANMITAQGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGLIDPRGWGEWEGDFALSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLHGRDWIPVTGVPFQAGI

Homology

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BLAST of MS005718 vs. NCBI nr
Match: XP_022146909.1 (pectinesterase [Momordica charantia])

HSP 1 Score: 952.2 bits (2460), Expect = 1.8e-273
Identity = 480/493 (97.36%), Postives = 480/493 (97.36%), Query Frame = 0


Query: 1   MPSPFAAFLLLLLAVASSISSFHDPDREVQLARTIVMEARDSVRGSVTGIGELKRRRFER 60
           MPSPFAAFLLLLLAVASSISSFHDPDREVQLARTIVMEARDSVRGSVTG GELKRRRFER
Sbjct: 1   MPSPFAAFLLLLLAVASSISSFHDPDREVQLARTIVMEARDSVRGSVTGTGELKRRRFER 60

Query: 61  RNFVEPALGDCTRLYEEADARLAELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGAA 120
           RNFVE ALG CTRLYEEADARLAELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGAA
Sbjct: 61  RNFVEAALGYCTRLYEEADARLAELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGAA 120

Query: 121 ADRKNNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKADYVV 180
           ADRKNNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKADYVV
Sbjct: 121 ADRKNNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKADYVV 180

Query: 181 AMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGDGAD 240
           AMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGDGAD
Sbjct: 181 AMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGDGAD 240

Query: 241 RTTITGSRNVPDGATTYSSATFAGVSGDGFLARDITFENTAGPEKHQAVALRVNSDLAVV 300
           RTTITGSRNVPDGATTYSSATF GVSGDGF ARDITFENTAGPEKHQAVALRVNSDLAVV
Sbjct: 241 RTTITGSRNVPDGATTYSSATF-GVSGDGFWARDITFENTAGPEKHQAVALRVNSDLAVV 300

Query: 301 YRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEILVRRPMRHQANMIT 360
           YRCTIKGYQD     SLRQFYRDCRIYGTVDFIFGNSAAVLQNCEI VRRPMRHQANMIT
Sbjct: 301 YRCTIKGYQD-----SLRQFYRDCRIYGTVDFIFGNSAAVLQNCEIFVRRPMRHQANMIT 360

Query: 361 AQGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGLIDP 420
           A GRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGLIDP
Sbjct: 361 AHGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGLIDP 420

Query: 421 RGWGEWEGDFALSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLHGRD 480
           RGWGEWEGDF LSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLHGRD
Sbjct: 421 RGWGEWEGDFPLSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLHGRD 480

Query: 481 WIPVTGVPFQAGI 494
           WIPVTGVPFQAGI
Sbjct: 481 WIPVTGVPFQAGI 487

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BLAST of MS005718 vs. NCBI nr
Match: XP_023540284.1 (probable pectinesterase/pectinesterase inhibitor 36 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 724.9 bits (1870), Expect = 4.6e-205
Identity = 376/496 (75.81%), Postives = 420/496 (84.68%), Query Frame = 0


Query: 1   MPSPFAAFLLLLLAV-ASSISSFHDPDREVQLARTIVMEARDSVRGSVTGIGELKRRRFE 60
           MP PFA  LLL LA+ ASSISS  +PD  V+L RT +++AR         I  LKRRRFE
Sbjct: 1   MPFPFATPLLLFLAIMASSISSLDEPD-AVRLLRTGIVKARSE-------ICNLKRRRFE 60

Query: 61  RRNFVEPALGDCTRLYEEADARLAELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGA 120
           R N V+ ALGDC  LYEEAD+RLA +L G NY+A+DAR W+SAAVANHRSC DGLEEVGA
Sbjct: 61  RGN-VQTALGDCHTLYEEADSRLAGMLVGENYSADDARIWLSAAVANHRSCLDGLEEVGA 120

Query: 121 --AADRKNNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKAD 180
             AAD  NNLT LL GAL LY+KIAAVEERNG +R E+++LT+N  I+LA WN ATSK D
Sbjct: 121 VPAAD-GNNLTVLLTGALHLYDKIAAVEERNGRERVEKERLTENGRINLATWNAATSKPD 180

Query: 181 YVVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGD 240
           YVVA+DGSGTH TIN AVAALAR GRRRRSERVVIYVKAGVY+ENVEIGMQLKNV +VGD
Sbjct: 181 YVVAVDGSGTHTTINGAVAALARDGRRRRSERVVIYVKAGVYKENVEIGMQLKNVMVVGD 240

Query: 241 GADRTTITGSRNVPDGATTYSSATFAGVSGDGFLARDITFENTAGPEKHQAVALRVNSDL 300
           GAD+T +TG+RNVPDGATTYSSATF GVSGDGF ARDITFENTAGP K QAVALR+NSDL
Sbjct: 241 GADKTIVTGNRNVPDGATTYSSATF-GVSGDGFWARDITFENTAGPGKQQAVALRLNSDL 300

Query: 301 AVVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEILVRRPMRHQAN 360
           AVVYRCTIKGYQD+LFLHSLRQFYRDC+IYGT+DFIFGN+AAVLQNC+I VRRP+RHQAN
Sbjct: 301 AVVYRCTIKGYQDTLFLHSLRQFYRDCQIYGTIDFIFGNAAAVLQNCDIFVRRPIRHQAN 360

Query: 361 MITAQGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGL 420
           MITAQGRDDPAE TGISI   RVRAAPE AAV+G+FRT+LGRPWKR+SRTVVM TDL+GL
Sbjct: 361 MITAQGRDDPAEATGISIISCRVRAAPELAAVKGDFRTYLGRPWKRFSRTVVMTTDLDGL 420

Query: 421 IDPRGWGEWEGDFALSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLH 480
           IDP+GWGEW GDFA+STLFY E+KNTG GSSTRFRV+WPGFH+ R   EA RFTV  FLH
Sbjct: 421 IDPKGWGEWRGDFAISTLFYAEFKNTGGGSSTRFRVHWPGFHVLRSAEEARRFTVAEFLH 480

Query: 481 GRDWIPVTGVPFQAGI 494
           G   IP TGVPF+AG+
Sbjct: 481 GDACIPATGVPFEAGV 485

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BLAST of MS005718 vs. NCBI nr
Match: XP_022974141.1 (probable pectinesterase/pectinesterase inhibitor 36 [Cucurbita maxima])

HSP 1 Score: 714.9 bits (1844), Expect = 4.7e-202
Identity = 368/495 (74.34%), Postives = 413/495 (83.43%), Query Frame = 0


Query: 1   MPSPFAAFLLLLLAV-ASSISSFHDPDREVQLARTIVMEARDSVRGSVTGIGELKRRRFE 60
           MP PFA  LLL LA+ ASSISS  +PD  V+L RT +++AR         I  +KRRRFE
Sbjct: 1   MPFPFATPLLLFLAIMASSISSLDEPD-AVRLLRTGIVKARSE-------IYNVKRRRFE 60

Query: 61  RRNFVEPALGDCTRLYEEADARLAELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGA 120
           R N V+ ALGDC  LYEEAD+RLA +L G NY+A+DAR W+SAAVANHRSC DGLEEVGA
Sbjct: 61  RGN-VQTALGDCHTLYEEADSRLAGMLVGENYSADDARIWLSAAVANHRSCLDGLEEVGA 120

Query: 121 AADRK-NNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKADY 180
                 NNLT LL GAL LY+KIAAVEER   +R E+++L +N  I+LAKWN ATSK DY
Sbjct: 121 VPSADGNNLTMLLTGALHLYDKIAAVEERKCWERVEKERLRENGRINLAKWNAATSKPDY 180

Query: 181 VVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGDG 240
           VVA DGSGT+ TIN AVAALAR GRRRRSERVVIYVKAGVY+ENVEIGMQLKNV +VGDG
Sbjct: 181 VVAKDGSGTYTTINGAVAALARDGRRRRSERVVIYVKAGVYKENVEIGMQLKNVMVVGDG 240

Query: 241 ADRTTITGSRNVPDGATTYSSATFAGVSGDGFLARDITFENTAGPEKHQAVALRVNSDLA 300
           AD+T +TG+RNVPDGATTYSSATF GVSGDGF ARDITFENTAGP K QAVALR+NSD  
Sbjct: 241 ADKTIVTGNRNVPDGATTYSSATF-GVSGDGFWARDITFENTAGPGKQQAVALRLNSDRV 300

Query: 301 VVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEILVRRPMRHQANM 360
           VVYRCTIKGYQD+LFLHSLRQFYRDC+IYGT+DFIFGN+AAVLQNC+I VRRP+RHQANM
Sbjct: 301 VVYRCTIKGYQDTLFLHSLRQFYRDCQIYGTIDFIFGNAAAVLQNCDIFVRRPIRHQANM 360

Query: 361 ITAQGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGLI 420
           ITAQGRDDPAE TGISI   RVRAAPE AAV+G+FRT+LGRPWKR+SRTV+M TDL+GLI
Sbjct: 361 ITAQGRDDPAEATGISIISCRVRAAPELAAVKGDFRTYLGRPWKRFSRTVIMTTDLDGLI 420

Query: 421 DPRGWGEWEGDFALSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLHG 480
           DP+GWGEW GDFALSTLFY E+KNTG GSSTRFRV+WPGFH+ R   EA RFTV GFL G
Sbjct: 421 DPKGWGEWRGDFALSTLFYAEFKNTGGGSSTRFRVHWPGFHVLRSAEEARRFTVAGFLRG 480

Query: 481 RDWIPVTGVPFQAGI 494
             WIP TGVPF+AG+
Sbjct: 481 DAWIPATGVPFEAGV 485

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BLAST of MS005718 vs. NCBI nr
Match: XP_022946489.1 (probable pectinesterase/pectinesterase inhibitor 36 [Cucurbita moschata])

HSP 1 Score: 714.5 bits (1843), Expect = 6.2e-202
Identity = 371/496 (74.80%), Postives = 415/496 (83.67%), Query Frame = 0


Query: 1   MPSPFAAFLLLLLAV-ASSISSFHDPDREVQLARTIVMEARDSVRGSVTGIGELKRRRFE 60
           MP PFA  LLL +A+ A SISS  +PD  V+L RT +++AR       + I  +KRRRFE
Sbjct: 1   MPFPFATPLLLFIAIMAFSISSLDEPD-AVRLLRTGIVKAR-------SDIYNVKRRRFE 60

Query: 61  RRNFVEPALGDCTRLYEEADARLAELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGA 120
           R N V+ ALGDC  LYEEAD+RLA +L G NY+A+DAR W+SAAVANHRSC DGLEEVGA
Sbjct: 61  RGN-VQAALGDCHTLYEEADSRLAAMLVGENYSADDARIWLSAAVANHRSCLDGLEEVGA 120

Query: 121 --AADRKNNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKAD 180
             AAD  NNLT LL GAL LY KIAAVEE    +R E+++  +N  I+LAKWN ATSK D
Sbjct: 121 VPAAD-GNNLTMLLTGALHLYGKIAAVEELTTWERVEKERSRENWRINLAKWNAATSKPD 180

Query: 181 YVVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGD 240
           YVVAMDGSGTH TIN AVAALAR GRRRRSERVVIYVKAGVY ENVEIGMQLKNV +VGD
Sbjct: 181 YVVAMDGSGTHTTINGAVAALARDGRRRRSERVVIYVKAGVYTENVEIGMQLKNVMVVGD 240

Query: 241 GADRTTITGSRNVPDGATTYSSATFAGVSGDGFLARDITFENTAGPEKHQAVALRVNSDL 300
           GAD+T +TG+RNVPDGATTYSSATF GVSGDGF  RDITFENTAGPEK QAVALR+NSDL
Sbjct: 241 GADKTIVTGNRNVPDGATTYSSATF-GVSGDGFWVRDITFENTAGPEKQQAVALRLNSDL 300

Query: 301 AVVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEILVRRPMRHQAN 360
           AVVYRCTIKGYQD+LFLHSLRQFYRDC+IYGT+DFIFGN+AAVLQNC+I VRRP+RHQAN
Sbjct: 301 AVVYRCTIKGYQDTLFLHSLRQFYRDCQIYGTIDFIFGNAAAVLQNCDIFVRRPIRHQAN 360

Query: 361 MITAQGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGL 420
           MITAQGRDDPAE TGISI   RVRAAPE AAV+G+FRT+LGRPWKR+SRTVVM TDL+GL
Sbjct: 361 MITAQGRDDPAEATGISIISCRVRAAPELAAVKGDFRTYLGRPWKRFSRTVVMTTDLDGL 420

Query: 421 IDPRGWGEWEGDFALSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLH 480
           IDP+GWGEW GDFA+STLFY E+KNTG GSSTRFRV+WPGFH+ R   EA RFTV  FLH
Sbjct: 421 IDPKGWGEWRGDFAISTLFYAEFKNTGGGSSTRFRVHWPGFHVLRSAEEARRFTVAEFLH 480

Query: 481 GRDWIPVTGVPFQAGI 494
           G  WIP TGVPF+AG+
Sbjct: 481 GDAWIPATGVPFEAGV 485

[Close]

BLAST of MS005718 vs. NCBI nr
Match: KAG6597209.1 (Methionine aminopeptidase 1A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 710.3 bits (1832), Expect = 1.2e-200
Identity = 363/479 (75.78%), Postives = 408/479 (85.18%), Query Frame = 0


Query: 15  VASSISSFHDPDREVQLARTIVMEARDSVRGSVTGIGELKRRRFERRNFVEPALGDCTRL 74
           +A SISS  +PD  V+L RT +++AR         I  +KRRRFER N V+ ALGDC  L
Sbjct: 1   MAFSISSLDEPD-AVRLLRTGIVKARSE-------IYNVKRRRFERGN-VQAALGDCHTL 60

Query: 75  YEEADARLAELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGA--AADRKNNLTALLA 134
           YEEAD+RLA +L G NY+A+DAR W+SAAVANHRSC DGLE+VGA  AAD  NNLT LL 
Sbjct: 61  YEEADSRLAGMLVGENYSADDARIWLSAAVANHRSCLDGLEKVGAVPAAD-GNNLTVLLT 120

Query: 135 GALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKADYVVAMDGSGTHRTIN 194
           GAL LY+KIAAVEERN  +R E+++L +N  I+LAKWN ATSK DYVVAMDGSGTH TIN
Sbjct: 121 GALHLYDKIAAVEERNVRERVEKERLRENGRINLAKWNAATSKPDYVVAMDGSGTHTTIN 180

Query: 195 DAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGDGADRTTITGSRNVPD 254
            AVAALAR GRRRRSERVVIYVKAGVY+ENVEIGMQLKNV +VGDGAD+T +TG+RNVPD
Sbjct: 181 GAVAALARDGRRRRSERVVIYVKAGVYKENVEIGMQLKNVMVVGDGADKTIVTGNRNVPD 240

Query: 255 GATTYSSATFAGVSGDGFLARDITFENTAGPEKHQAVALRVNSDLAVVYRCTIKGYQDSL 314
           GATTYSSATF GVSGDGF ARDITFENTAGPEK QAVALR+NSDLA+VYRCTIKGYQD+L
Sbjct: 241 GATTYSSATF-GVSGDGFWARDITFENTAGPEKQQAVALRLNSDLAIVYRCTIKGYQDTL 300

Query: 315 FLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEILVRRPMRHQANMITAQGRDDPAENTG 374
           FLHSLRQFYRDC+IYGT+DFIFGN+AAVLQNC+I VRRP+RHQANMITAQGRDDPAE TG
Sbjct: 301 FLHSLRQFYRDCQIYGTIDFIFGNAAAVLQNCDIFVRRPIRHQANMITAQGRDDPAEATG 360

Query: 375 ISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGLIDPRGWGEWEGDFAL 434
           ISI   RVRAAPE AAV+G+FRT+LGRPWKR+SRTVVM TDL+GLIDP+GWGEW GDFA+
Sbjct: 361 ISIISCRVRAAPELAAVKGDFRTYLGRPWKRFSRTVVMTTDLDGLIDPKGWGEWRGDFAI 420

Query: 435 STLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLHGRDWIPVTGVPFQA 492
           STLFY E+KNTG GSSTRFRV+WPGFH+ R   EA RFTV  FLHG  WIP TGVPF+A
Sbjct: 421 STLFYAEFKNTGGGSSTRFRVHWPGFHVLRSAEEARRFTVAEFLHGDAWIPATGVPFEA 468

[Close]

BLAST of MS005718 vs. ExPASy Swiss-Prot
Match: Q84R10 (Probable pectinesterase/pectinesterase inhibitor 36 OS=Arabidopsis thaliana OX=3702 GN=PME36 PE=2 SV=2)

HSP 1 Score: 536.2 bits (1380), Expect = 3.9e-151
Identity = 275/513 (53.61%), Postives = 359/513 (69.98%), Query Frame = 0


Query: 9   LLLLLAVASSISSFHDPDREV-QLARTIVMEARDSVRGSVTGIGELKRRRFERRNFVEPA 68
           ++  LA+A+ I++ +  + +V ++ART V+EA+ S  GS   +   +       ++ +  
Sbjct: 13  IMFFLAIAAVITASNTAELDVLEMARTAVVEAKTSF-GS---MAVTEATSEVAGSYYKLG 72

Query: 69  LGDCTRLYEEADARLAELLFG-GNYTAEDARTWISAAVANHRSCTDGL--EEVGAAADRK 128
           L +C +LY+E++ARL++L+    N+T ED RTW+S  +ANH +C DGL  +  G      
Sbjct: 73  LSECEKLYDESEARLSKLVVDHENFTVEDVRTWLSGVLANHHTCLDGLIQQRQGHKPLVH 132

Query: 129 NNLTALLAGALRLYEKI---------AAVEERNGLKRWEEQKLTKNRGID---------- 188
           +N+T +L  AL  Y+K              + +G  R + +    N G            
Sbjct: 133 SNVTFVLHEALAFYKKSRGHMKKRLHGPARQGHGPTRPKHRPTRPNHGPGRSHHGPSRPN 192

Query: 189 -----LAKWNPATSKADYVVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYR 248
                L  WNP +S+AD+VVA DGS THRTIN A+AA++R G+ R + RV+IY+KAGVY 
Sbjct: 193 QNGGMLVSWNPTSSRADFVVARDGSATHRTINQALAAVSRMGKSRLN-RVIIYIKAGVYN 252

Query: 249 ENVEIGMQLKNVFIVGDGADRTTITGSRNVPDGATTYSSATFAGVSGDGFLARDITFENT 308
           E +EI   +KN+ +VGDG DRT +T +RNVPDG+TTY SATF GVSGDGF ARDITFENT
Sbjct: 253 EKIEIDRHMKNIMLVGDGMDRTIVTNNRNVPDGSTTYGSATF-GVSGDGFWARDITFENT 312

Query: 309 AGPEKHQAVALRVNSDLAVVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAV 368
           AGP KHQAVALRV+SDL++ YRC+ KGYQD+LF HSLRQFYRDC IYGT+DFIFG++AAV
Sbjct: 313 AGPHKHQAVALRVSSDLSLFYRCSFKGYQDTLFTHSLRQFYRDCHIYGTIDFIFGDAAAV 372

Query: 369 LQNCEILVRRPMRHQANMITAQGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRP 428
            QNC+I VRRPM HQ NMITAQGRDDP  N+GISI  SR+RAAPEF AV+G F+++LGRP
Sbjct: 373 FQNCDIFVRRPMDHQGNMITAQGRDDPHTNSGISIQHSRIRAAPEFEAVKGRFKSYLGRP 432

Query: 429 WKRYSRTVVMATDLEGLIDPRGWGEWEGDFALSTLFYGEYKNTGRGSSTRFRVNWPGFHI 488
           WK+YSRTV + TD++ LIDPRGW EW G +ALSTL+YGE+ NTG G+ T  RVNWPGFH+
Sbjct: 433 WKKYSRTVFLKTDIDELIDPRGWREWSGSYALSTLYYGEFMNTGAGAGTGRRVNWPGFHV 492

Query: 489 FRGDLEAGRFTVGGFLHGRDWIPVTGVPFQAGI 494
            RG+ EA  FTV  F+ G  WIP+TGVPF AG+
Sbjct: 493 LRGEEEASPFTVSRFIQGDSWIPITGVPFSAGV 519

[Close]

BLAST of MS005718 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 2.6e-110
Identity = 231/504 (45.83%), Postives = 302/504 (59.92%), Query Frame = 0


Query: 2   PSPFAAFLLLLLAVAS--SISSFHDPDREVQLARTIVMEARDSVRGSVTGIGELKRRRFE 61
           P P   FL     V S    + F+    ++ L R    ++R    GS        + R E
Sbjct: 38  PQPCEYFLTQKTDVTSIKQDTDFYKISLQLALERATTAQSRTYTLGS--------KCRNE 97

Query: 62  RRNFVEPALGDCTRLYEEADARLAELLFGG-NYTAEDARTWISAAVANHRSCTDGLEEVG 121
           R    + A  DC  LYE    +L +        T  D +TW+S A+ N  +C   LE++G
Sbjct: 98  RE---KAAWEDCRELYELTVLKLNQTSNSSPGCTKVDKQTWLSTALTNLETCRASLEDLG 157

Query: 122 AAAD----RKNNLTALLAGALRLYEKIAAVEE--RNGLKRW---EEQKLTKNRGIDLAKW 181
                     NN+T L++  L L  K+   E   ++G   W    ++KL +         
Sbjct: 158 VPEYVLPLLSNNVTKLISNTLSL-NKVPYNEPSYKDGFPTWVKPGDRKLLQ--------- 217

Query: 182 NPATSKADYVVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQL 241
              T +A+ VVA DGSG  +TI +AVAA +R+G      R VIY+KAG Y EN+E+  +L
Sbjct: 218 --TTPRANIVVAQDGSGNVKTIQEAVAAASRAG----GSRYVIYIKAGTYNENIEV--KL 277

Query: 242 KNVFIVGDGADRTTITGSRNVPDGATTYSSATFAGVSGDGFLARDITFENTAGPEKHQAV 301
           KN+  VGDG  +T ITGS++V  GATT+ SAT A V GD F+ARDIT  NTAGP  HQAV
Sbjct: 278 KNIMFVGDGIGKTIITGSKSVGGGATTFKSATVA-VVGDNFIARDITIRNTAGPNNHQAV 337

Query: 302 ALRVNSDLAVVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEILVR 361
           ALR  SDL+V YRC+ +GYQD+L++HS RQFYR+C IYGTVDFIFGN+A VLQNC I  R
Sbjct: 338 ALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFAR 397

Query: 362 RPMRHQANMITAQGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVV 421
           +P  ++ N +TAQGR DP ++TGI IH  RV AA +   VQ   +TFLGRPWK+YSRTV 
Sbjct: 398 KP-PNRTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVY 457

Query: 422 MATDLEGLIDPRGWGEWEGDFALSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGR 481
           + T L+ LI+P GW EW GDFAL+TL+Y EY NTG GSST  RV W G+H+     +  +
Sbjct: 458 IKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQ 510

Query: 482 FTVGGFLHGRDWIPVTGVPFQAGI 494
           FTVG F+ G  W+P T VPF +G+
Sbjct: 518 FTVGNFIAGNSWLPATNVPFTSGL 510

[Close]

BLAST of MS005718 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 389.8 bits (1000), Expect = 4.6e-107
Identity = 216/432 (50.00%), Postives = 278/432 (64.35%), Query Frame = 0


Query: 70  DCTRLYEEADARLAELLFGGNYTAE-DARTWISAAVANHRSCTDGLEEVGAA----ADRK 129
           DC +LY+   +++ E +      ++ DA+TW+S A+ N  +C  G  E+G          
Sbjct: 99  DCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFLELGVTDIVLPLMS 158

Query: 130 NNLTALLAGALRLYEKIA---AVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKADYVVA 189
           NN++ LL   L +  K+       E++G   W +         D      +T K + VVA
Sbjct: 159 NNVSNLLCNTLAI-NKVPFNYTPPEKDGFPSWVKPG-------DRKLLQSSTPKDNAVVA 218

Query: 190 MDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGDGADR 249
            DGSG  +TI +A+ A + SG      R VIYVK GVY EN+EI  + KNV + GDG  +
Sbjct: 219 KDGSGNFKTIKEAIDAASGSG------RFVIYVKQGVYSENLEI--RKKNVMLRGDGIGK 278

Query: 250 TTITGSRNVPDGATTYSSATFAGVSGDGFLARDITFENTAGPEKHQAVALRVNSDLAVVY 309
           T ITGS++V  G TT++SAT A V GDGF+AR ITF NTAG    QAVALR  SDL+V Y
Sbjct: 279 TIITGSKSVGGGTTTFNSATVAAV-GDGFIARGITFRNTAGASNEQAVALRSGSDLSVFY 338

Query: 310 RCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEILVRRPMRHQANMITA 369
           +C+ + YQD+L++HS RQFYRDC +YGTVDFIFGN+AAVLQNC I  RRP R + N ITA
Sbjct: 339 QCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRP-RSKTNTITA 398

Query: 370 QGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGLIDPR 429
           QGR DP +NTGI IH SRV AA +   V G  +T+LGRPW++YSRTV M T L+ LIDPR
Sbjct: 399 QGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPR 458

Query: 430 GWGEWEGDFALSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLHGRDW 489
           GW EW+G+FAL TLFY E++NTG G+ST  RV WPGF +     EA +FTVG FL G  W
Sbjct: 459 GWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSW 511

Query: 490 IPVTGVPFQAGI 494
           IP + VPF +G+
Sbjct: 519 IP-SSVPFTSGL 511

[Close]

BLAST of MS005718 vs. ExPASy Swiss-Prot
Match: Q9FK05 (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3702 GN=PME61 PE=2 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 1.7e-106
Identity = 205/454 (45.15%), Postives = 274/454 (60.35%), Query Frame = 0


Query: 64  VEPALGDCTRLYEEADARLAELL-----FGGNYTAEDARTWISAAVANHRSCTDGLEE-- 123
           V  A   C  L +++   L   L       G+ +  D  TW+S+A+ NH +CTDG +E  
Sbjct: 137 VRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTNHDTCTDGFDEIE 196

Query: 124 ----------VGAAADRK---NNLTALLAGALRLYEKIAAVEERNGLKRWEEQKL---TK 183
                     +GA  D     +N  A+ AG ++    +  V  R  L   E ++L    K
Sbjct: 197 GQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEETEELPNWLK 256

Query: 184 NRGIDLAKWNPATSKADYVVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVY- 243
               +L     +  +AD  V+ DGSGT +TI +A+    +      S R VIYVKAG Y 
Sbjct: 257 REDRELLGTPTSAIQADITVSKDGSGTFKTIAEAI----KKAPEHSSRRFVIYVKAGRYE 316

Query: 244 RENVEIGMQLKNVFIVGDGADRTTITGSRNVPDGATTYSSATFAGVSGDGFLARDITFEN 303
            EN+++G +  N+  +GDG  +T ITG +++ D  TT+ +ATFA  +G GF+ RD+TFEN
Sbjct: 317 EENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFA-ATGAGFIVRDMTFEN 376

Query: 304 TAGPEKHQAVALRVNSDLAVVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAA 363
            AGP KHQAVALRV  D AVVYRC I GYQD+L++HS RQF+R+C IYGTVDFIFGN+A 
Sbjct: 377 YAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFIFGNAAV 436

Query: 364 VLQNCEILVRRPMRHQANMITAQGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGR 423
           +LQ+C I  R+PM  Q   ITAQ R DP +NTGISIH  ++ A P+  A +G + T+LGR
Sbjct: 437 ILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGSYPTYLGR 496

Query: 424 PWKRYSRTVVMATDLEGLIDPRGWGEWEGDFALSTLFYGEYKNTGRGSSTRFRVNWPGFH 483
           PWK YSR V M +D+   IDPRGW EW G FAL +L+YGEY N G GS    RV WPG+H
Sbjct: 497 PWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIGQRVKWPGYH 556

Query: 484 IFRGDLEAGRFTVGGFLHGRDWIPVTGVPFQAGI 494
           +    +EA +FTV  F+ G  W+P TGV F +G+
Sbjct: 557 VITSTVEASKFTVAQFISGSSWLPSTGVSFFSGL 585

[Close]

BLAST of MS005718 vs. ExPASy Swiss-Prot
Match: O49298 (Probable pectinesterase/pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=PME6 PE=2 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 5.0e-106
Identity = 218/464 (46.98%), Postives = 280/464 (60.34%), Query Frame = 0


Query: 67  ALGDCTRLYEEADARL--AELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGAAADRK 126
           AL DC  LYE+   +L  +   +G   +  D +T +SAA+AN  +C +G  +    +   
Sbjct: 103 ALFDCLELYEDTIDQLNHSRRSYGQYSSPHDRQTSLSAAIANQDTCRNGFRDFKLTSSYS 162

Query: 127 --------NNLTALLAGALRLYEKIA---AVEER---NGLKRWEEQKLTKNRGI------ 186
                    NLT  ++ +L + +  A   AV E+    G  ++ +Q+ +   G       
Sbjct: 163 KYFPVQFHRNLTKSISNSLAVTKAAAEAEAVAEKYPSTGFTKFSKQRSSAGGGSHRRLLL 222

Query: 187 ----DLAKWNP-----------ATSKADYVVAMDGSGTHRTINDAVAALARSGRRRRSER 246
                   W P            T+KAD VVA DGSG + +I  AV A A+    RR++R
Sbjct: 223 FSDEKFPSWFPLSDRKLLEDSKTTAKADLVVAKDGSGHYTSIQQAVNAAAK--LPRRNQR 282

Query: 247 VVIYVKAGVYRENVEIGMQLKNVFIVGDGADRTTITGSRNVPDGATTYSSATFAGVSGDG 306
           +VIYVKAGVYRENV I   +KNV ++GDG D T +TG+RNV DG TT+ SATFA VSG+G
Sbjct: 283 LVIYVKAGVYRENVVIKKSIKNVMVIGDGIDSTIVTGNRNVQDGTTTFRSATFA-VSGNG 342

Query: 307 FLARDITFENTAGPEKHQAVALRVNSDLAVVYRCTIKGYQDSLFLHSLRQFYRDCRIYGT 366
           F+A+ ITFENTAGPEKHQAVALR +SD +V Y C+ KGYQD+L+LHS RQF R+C IYGT
Sbjct: 343 FIAQGITFENTAGPEKHQAVALRSSSDFSVFYACSFKGYQDTLYLHSSRQFLRNCNIYGT 402

Query: 367 VDFIFGNSAAVLQNCEILVRRPMRHQANMITAQGRDDPAENTGISIHGSRVRAAPEFAAV 426
           VDFIFG++ A+LQNC I  R+PM  Q N ITAQ R +P E TG  I  S V  A E    
Sbjct: 403 VDFIFGDATAILQNCNIYARKPMSGQKNTITAQSRKEPDETTGFVIQSSTVATASE---- 462

Query: 427 QGEFRTFLGRPWKRYSRTVVMATDLEGLIDPRGWGEWEGDFALSTLFYGEYKNTGRGSST 486
                T+LGRPW+ +SRTV M  +L  L+ P GW  W G FALSTL+YGEY NTG G+S 
Sbjct: 463 -----TYLGRPWRSHSRTVFMKCNLGALVSPAGWLPWSGSFALSTLYYGEYGNTGAGASV 522

Query: 487 RFRVNWPGFHIFRGDLEAGRFTVGGFLHGRDWIPVTGVPFQAGI 494
             RV WPG+H+ +   EA +FTV  FL G  WI  TGVP   G+
Sbjct: 523 SGRVKWPGYHVIKTVTEAEKFTVENFLDGNYWITATGVPVNDGL 554

[Close]

BLAST of MS005718 vs. ExPASy TrEMBL
Match: A0A6J1CZF5 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111015995 PE=3 SV=1)

HSP 1 Score: 952.2 bits (2460), Expect = 8.5e-274
Identity = 480/493 (97.36%), Postives = 480/493 (97.36%), Query Frame = 0


Query: 1   MPSPFAAFLLLLLAVASSISSFHDPDREVQLARTIVMEARDSVRGSVTGIGELKRRRFER 60
           MPSPFAAFLLLLLAVASSISSFHDPDREVQLARTIVMEARDSVRGSVTG GELKRRRFER
Sbjct: 1   MPSPFAAFLLLLLAVASSISSFHDPDREVQLARTIVMEARDSVRGSVTGTGELKRRRFER 60

Query: 61  RNFVEPALGDCTRLYEEADARLAELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGAA 120
           RNFVE ALG CTRLYEEADARLAELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGAA
Sbjct: 61  RNFVEAALGYCTRLYEEADARLAELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGAA 120

Query: 121 ADRKNNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKADYVV 180
           ADRKNNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKADYVV
Sbjct: 121 ADRKNNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKADYVV 180

Query: 181 AMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGDGAD 240
           AMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGDGAD
Sbjct: 181 AMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGDGAD 240

Query: 241 RTTITGSRNVPDGATTYSSATFAGVSGDGFLARDITFENTAGPEKHQAVALRVNSDLAVV 300
           RTTITGSRNVPDGATTYSSATF GVSGDGF ARDITFENTAGPEKHQAVALRVNSDLAVV
Sbjct: 241 RTTITGSRNVPDGATTYSSATF-GVSGDGFWARDITFENTAGPEKHQAVALRVNSDLAVV 300

Query: 301 YRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEILVRRPMRHQANMIT 360
           YRCTIKGYQD     SLRQFYRDCRIYGTVDFIFGNSAAVLQNCEI VRRPMRHQANMIT
Sbjct: 301 YRCTIKGYQD-----SLRQFYRDCRIYGTVDFIFGNSAAVLQNCEIFVRRPMRHQANMIT 360

Query: 361 AQGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGLIDP 420
           A GRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGLIDP
Sbjct: 361 AHGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGLIDP 420

Query: 421 RGWGEWEGDFALSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLHGRD 480
           RGWGEWEGDF LSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLHGRD
Sbjct: 421 RGWGEWEGDFPLSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLHGRD 480

Query: 481 WIPVTGVPFQAGI 494
           WIPVTGVPFQAGI
Sbjct: 481 WIPVTGVPFQAGI 487

[Close]

BLAST of MS005718 vs. ExPASy TrEMBL
Match: A0A6J1IAJ0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472734 PE=3 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 2.3e-202
Identity = 368/495 (74.34%), Postives = 413/495 (83.43%), Query Frame = 0


Query: 1   MPSPFAAFLLLLLAV-ASSISSFHDPDREVQLARTIVMEARDSVRGSVTGIGELKRRRFE 60
           MP PFA  LLL LA+ ASSISS  +PD  V+L RT +++AR         I  +KRRRFE
Sbjct: 1   MPFPFATPLLLFLAIMASSISSLDEPD-AVRLLRTGIVKARSE-------IYNVKRRRFE 60

Query: 61  RRNFVEPALGDCTRLYEEADARLAELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGA 120
           R N V+ ALGDC  LYEEAD+RLA +L G NY+A+DAR W+SAAVANHRSC DGLEEVGA
Sbjct: 61  RGN-VQTALGDCHTLYEEADSRLAGMLVGENYSADDARIWLSAAVANHRSCLDGLEEVGA 120

Query: 121 AADRK-NNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKADY 180
                 NNLT LL GAL LY+KIAAVEER   +R E+++L +N  I+LAKWN ATSK DY
Sbjct: 121 VPSADGNNLTMLLTGALHLYDKIAAVEERKCWERVEKERLRENGRINLAKWNAATSKPDY 180

Query: 181 VVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGDG 240
           VVA DGSGT+ TIN AVAALAR GRRRRSERVVIYVKAGVY+ENVEIGMQLKNV +VGDG
Sbjct: 181 VVAKDGSGTYTTINGAVAALARDGRRRRSERVVIYVKAGVYKENVEIGMQLKNVMVVGDG 240

Query: 241 ADRTTITGSRNVPDGATTYSSATFAGVSGDGFLARDITFENTAGPEKHQAVALRVNSDLA 300
           AD+T +TG+RNVPDGATTYSSATF GVSGDGF ARDITFENTAGP K QAVALR+NSD  
Sbjct: 241 ADKTIVTGNRNVPDGATTYSSATF-GVSGDGFWARDITFENTAGPGKQQAVALRLNSDRV 300

Query: 301 VVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEILVRRPMRHQANM 360
           VVYRCTIKGYQD+LFLHSLRQFYRDC+IYGT+DFIFGN+AAVLQNC+I VRRP+RHQANM
Sbjct: 301 VVYRCTIKGYQDTLFLHSLRQFYRDCQIYGTIDFIFGNAAAVLQNCDIFVRRPIRHQANM 360

Query: 361 ITAQGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGLI 420
           ITAQGRDDPAE TGISI   RVRAAPE AAV+G+FRT+LGRPWKR+SRTV+M TDL+GLI
Sbjct: 361 ITAQGRDDPAEATGISIISCRVRAAPELAAVKGDFRTYLGRPWKRFSRTVIMTTDLDGLI 420

Query: 421 DPRGWGEWEGDFALSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLHG 480
           DP+GWGEW GDFALSTLFY E+KNTG GSSTRFRV+WPGFH+ R   EA RFTV GFL G
Sbjct: 421 DPKGWGEWRGDFALSTLFYAEFKNTGGGSSTRFRVHWPGFHVLRSAEEARRFTVAGFLRG 480

Query: 481 RDWIPVTGVPFQAGI 494
             WIP TGVPF+AG+
Sbjct: 481 DAWIPATGVPFEAGV 485

[Close]

BLAST of MS005718 vs. ExPASy TrEMBL
Match: A0A6J1G3Y9 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111450531 PE=3 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 3.0e-202
Identity = 371/496 (74.80%), Postives = 415/496 (83.67%), Query Frame = 0


Query: 1   MPSPFAAFLLLLLAV-ASSISSFHDPDREVQLARTIVMEARDSVRGSVTGIGELKRRRFE 60
           MP PFA  LLL +A+ A SISS  +PD  V+L RT +++AR       + I  +KRRRFE
Sbjct: 1   MPFPFATPLLLFIAIMAFSISSLDEPD-AVRLLRTGIVKAR-------SDIYNVKRRRFE 60

Query: 61  RRNFVEPALGDCTRLYEEADARLAELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGA 120
           R N V+ ALGDC  LYEEAD+RLA +L G NY+A+DAR W+SAAVANHRSC DGLEEVGA
Sbjct: 61  RGN-VQAALGDCHTLYEEADSRLAAMLVGENYSADDARIWLSAAVANHRSCLDGLEEVGA 120

Query: 121 --AADRKNNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKAD 180
             AAD  NNLT LL GAL LY KIAAVEE    +R E+++  +N  I+LAKWN ATSK D
Sbjct: 121 VPAAD-GNNLTMLLTGALHLYGKIAAVEELTTWERVEKERSRENWRINLAKWNAATSKPD 180

Query: 181 YVVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGD 240
           YVVAMDGSGTH TIN AVAALAR GRRRRSERVVIYVKAGVY ENVEIGMQLKNV +VGD
Sbjct: 181 YVVAMDGSGTHTTINGAVAALARDGRRRRSERVVIYVKAGVYTENVEIGMQLKNVMVVGD 240

Query: 241 GADRTTITGSRNVPDGATTYSSATFAGVSGDGFLARDITFENTAGPEKHQAVALRVNSDL 300
           GAD+T +TG+RNVPDGATTYSSATF GVSGDGF  RDITFENTAGPEK QAVALR+NSDL
Sbjct: 241 GADKTIVTGNRNVPDGATTYSSATF-GVSGDGFWVRDITFENTAGPEKQQAVALRLNSDL 300

Query: 301 AVVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEILVRRPMRHQAN 360
           AVVYRCTIKGYQD+LFLHSLRQFYRDC+IYGT+DFIFGN+AAVLQNC+I VRRP+RHQAN
Sbjct: 301 AVVYRCTIKGYQDTLFLHSLRQFYRDCQIYGTIDFIFGNAAAVLQNCDIFVRRPIRHQAN 360

Query: 361 MITAQGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGL 420
           MITAQGRDDPAE TGISI   RVRAAPE AAV+G+FRT+LGRPWKR+SRTVVM TDL+GL
Sbjct: 361 MITAQGRDDPAEATGISIISCRVRAAPELAAVKGDFRTYLGRPWKRFSRTVVMTTDLDGL 420

Query: 421 IDPRGWGEWEGDFALSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLH 480
           IDP+GWGEW GDFA+STLFY E+KNTG GSSTRFRV+WPGFH+ R   EA RFTV  FLH
Sbjct: 421 IDPKGWGEWRGDFAISTLFYAEFKNTGGGSSTRFRVHWPGFHVLRSAEEARRFTVAEFLH 480

Query: 481 GRDWIPVTGVPFQAGI 494
           G  WIP TGVPF+AG+
Sbjct: 481 GDAWIPATGVPFEAGV 485

[Close]

BLAST of MS005718 vs. ExPASy TrEMBL
Match: A0A5D3D3J9 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003760 PE=3 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 1.8e-194
Identity = 353/498 (70.88%), Postives = 403/498 (80.92%), Query Frame = 0


Query: 1   MPSPFAAFLLLLLAVASSISSFHDPDRE-VQLARTIVMEARDSVRGSVTGIGELKRRRFE 60
           MP PF  FLLLL     + S+F  P  + V+LA+T        VR +VT IG LKRRRF 
Sbjct: 1   MPFPFTTFLLLLCLPFVASSNFSLPALDSVRLAQT-------GVREAVTEIGNLKRRRFG 60

Query: 61  RRNFVEPALGDCTRLYEEADARLAELLFGGN--YTAEDARTWISAAVANHRSCTDGLEEV 120
             N +  AL DC +LYEEA+ RLA +L GG   Y+AED R W+SAA+ANHRSC DGLEEV
Sbjct: 61  DGNHLLAALRDCAKLYEEAEWRLAGMLVGGEKYYSAEDGRMWVSAAMANHRSCLDGLEEV 120

Query: 121 GA--AADRKNNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSK 180
            A  AA   +NLT +L GAL LY+KIAAVE+RNG KR E++K  +NRGI+LA WNPATSK
Sbjct: 121 DADGAAVGGHNLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWNPATSK 180

Query: 181 ADYVVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIV 240
           ADYVVA DGSGTH TIN AVAALA +GRRR   R+VIYVKAGVYRENVEIG+QLKNV +V
Sbjct: 181 ADYVVAKDGSGTHTTINRAVAALASTGRRRGGGRIVIYVKAGVYRENVEIGIQLKNVMLV 240

Query: 241 GDGADRTTITGSRNVPDGATTYSSATFAGVSGDGFLARDITFENTAGPEKHQAVALRVNS 300
           GDG D+T +TGSRNVPDGATTY+SATF GVSGDGF ARDITFENTAGPEK QAVALR+NS
Sbjct: 241 GDGIDKTIVTGSRNVPDGATTYNSATF-GVSGDGFWARDITFENTAGPEKQQAVALRLNS 300

Query: 301 DLAVVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEILVRRPMRHQ 360
           DLAVVYRC IKGYQD+LFLHSLRQFYRDC+IYGT+DFIFGNSAAVLQNCEI VR+P RHQ
Sbjct: 301 DLAVVYRCAIKGYQDTLFLHSLRQFYRDCKIYGTIDFIFGNSAAVLQNCEIFVRQPRRHQ 360

Query: 361 ANMITAQGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLE 420
           ANMITAQGRDDPAE TGISI  SRV+  P+FA V+ ++RT+LGRPWKR+SRTV+M TDL+
Sbjct: 361 ANMITAQGRDDPAEATGISILNSRVQPTPDFAPVKAQYRTYLGRPWKRFSRTVIMGTDLD 420

Query: 421 GLIDPRGWGEWEGDFALSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGF 480
           GLIDP+GWGEW G+FA+STLFY E+KN G GSSTRFRVNWPGFH+ R   EA  FTV  F
Sbjct: 421 GLIDPKGWGEWTGNFAISTLFYAEFKNRGDGSSTRFRVNWPGFHVLRSAEEARPFTVAEF 480

Query: 481 LHGRDWIPVTGVPFQAGI 494
           LHG DWIP +GV F+AG+
Sbjct: 481 LHGGDWIPASGVSFEAGV 490

[Close]

BLAST of MS005718 vs. ExPASy TrEMBL
Match: A0A1S3AVG1 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483369 PE=3 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 1.8e-194
Identity = 354/498 (71.08%), Postives = 403/498 (80.92%), Query Frame = 0


Query: 1   MPSPFAAFLLLLLAVASSISSFHDPDRE-VQLARTIVMEARDSVRGSVTGIGELKRRRFE 60
           MP PF  FLLLL     + S+F  P  + V+LA+T        VR +VT IG LKRRRF 
Sbjct: 1   MPFPFTTFLLLLCLPFVASSNFSLPALDSVRLAQT-------GVREAVTEIGNLKRRRFG 60

Query: 61  RRNFVEPALGDCTRLYEEADARLAELLFGGN--YTAEDARTWISAAVANHRSCTDGLEEV 120
             N +  AL DC +LYEEA+ RLA +L GG   Y+AED R W+SAA+ANHRSC DGLEEV
Sbjct: 61  DGNHLLAALRDCAKLYEEAEWRLAGMLVGGEKYYSAEDGRMWVSAAMANHRSCLDGLEEV 120

Query: 121 GA--AADRKNNLTALLAGALRLYEKIAAVEERNGLKRWEEQKLTKNRGIDLAKWNPATSK 180
            A  AA   +NLT +L GAL LY+KIAAVE+RNG KR E++K  +NRGI+LA WNPATSK
Sbjct: 121 DADGAAVGGHNLTVMLTGALHLYDKIAAVEKRNGWKRLEKRKWRENRGINLATWNPATSK 180

Query: 181 ADYVVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIV 240
           ADYVVA DGSGTH TIN AVAALA +GRRR   R+VIYVKAGVYRENVEIG+QLKNV +V
Sbjct: 181 ADYVVAKDGSGTHTTINRAVAALASTGRRRGGGRIVIYVKAGVYRENVEIGIQLKNVMLV 240

Query: 241 GDGADRTTITGSRNVPDGATTYSSATFAGVSGDGFLARDITFENTAGPEKHQAVALRVNS 300
           GDG D+T +TGSRNVPDGATTY+SATF GVSGDGF ARDITFENTAGPEK QAVALR+NS
Sbjct: 241 GDGIDKTIVTGSRNVPDGATTYNSATF-GVSGDGFWARDITFENTAGPEKQQAVALRLNS 300

Query: 301 DLAVVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEILVRRPMRHQ 360
           DLAVVYRC IKGYQD+LFLHSLRQFYRDC+IYGT+DFIFGNSAAVLQNCEI VR+P RHQ
Sbjct: 301 DLAVVYRCAIKGYQDTLFLHSLRQFYRDCKIYGTIDFIFGNSAAVLQNCEIFVRQPRRHQ 360

Query: 361 ANMITAQGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLE 420
           ANMITAQGRDDPAE TGISI  SRV+  P+FA V+ ++RT+LGRPWKR+SRTVVM TDL+
Sbjct: 361 ANMITAQGRDDPAEATGISILNSRVQPTPDFAPVKAQYRTYLGRPWKRFSRTVVMGTDLD 420

Query: 421 GLIDPRGWGEWEGDFALSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGF 480
           GLIDP+GWGEW G+FA+STLFY E+KN G GSSTRFRVNWPGFH+ R   EA  FTV  F
Sbjct: 421 GLIDPKGWGEWTGNFAISTLFYAEFKNRGDGSSTRFRVNWPGFHVLRSADEARPFTVAEF 480

Query: 481 LHGRDWIPVTGVPFQAGI 494
           LHG DWIP +GV F+AG+
Sbjct: 481 LHGGDWIPASGVSFEAGV 490

[Close]

BLAST of MS005718 vs. TAIR 10
Match: AT3G60730.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 536.2 bits (1380), Expect = 2.8e-152
Identity = 275/513 (53.61%), Postives = 359/513 (69.98%), Query Frame = 0


Query: 9   LLLLLAVASSISSFHDPDREV-QLARTIVMEARDSVRGSVTGIGELKRRRFERRNFVEPA 68
           ++  LA+A+ I++ +  + +V ++ART V+EA+ S  GS   +   +       ++ +  
Sbjct: 13  IMFFLAIAAVITASNTAELDVLEMARTAVVEAKTSF-GS---MAVTEATSEVAGSYYKLG 72

Query: 69  LGDCTRLYEEADARLAELLFG-GNYTAEDARTWISAAVANHRSCTDGL--EEVGAAADRK 128
           L +C +LY+E++ARL++L+    N+T ED RTW+S  +ANH +C DGL  +  G      
Sbjct: 73  LSECEKLYDESEARLSKLVVDHENFTVEDVRTWLSGVLANHHTCLDGLIQQRQGHKPLVH 132

Query: 129 NNLTALLAGALRLYEKI---------AAVEERNGLKRWEEQKLTKNRGID---------- 188
           +N+T +L  AL  Y+K              + +G  R + +    N G            
Sbjct: 133 SNVTFVLHEALAFYKKSRGHMKKRLHGPARQGHGPTRPKHRPTRPNHGPGRSHHGPSRPN 192

Query: 189 -----LAKWNPATSKADYVVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYR 248
                L  WNP +S+AD+VVA DGS THRTIN A+AA++R G+ R + RV+IY+KAGVY 
Sbjct: 193 QNGGMLVSWNPTSSRADFVVARDGSATHRTINQALAAVSRMGKSRLN-RVIIYIKAGVYN 252

Query: 249 ENVEIGMQLKNVFIVGDGADRTTITGSRNVPDGATTYSSATFAGVSGDGFLARDITFENT 308
           E +EI   +KN+ +VGDG DRT +T +RNVPDG+TTY SATF GVSGDGF ARDITFENT
Sbjct: 253 EKIEIDRHMKNIMLVGDGMDRTIVTNNRNVPDGSTTYGSATF-GVSGDGFWARDITFENT 312

Query: 309 AGPEKHQAVALRVNSDLAVVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAV 368
           AGP KHQAVALRV+SDL++ YRC+ KGYQD+LF HSLRQFYRDC IYGT+DFIFG++AAV
Sbjct: 313 AGPHKHQAVALRVSSDLSLFYRCSFKGYQDTLFTHSLRQFYRDCHIYGTIDFIFGDAAAV 372

Query: 369 LQNCEILVRRPMRHQANMITAQGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRP 428
            QNC+I VRRPM HQ NMITAQGRDDP  N+GISI  SR+RAAPEF AV+G F+++LGRP
Sbjct: 373 FQNCDIFVRRPMDHQGNMITAQGRDDPHTNSGISIQHSRIRAAPEFEAVKGRFKSYLGRP 432

Query: 429 WKRYSRTVVMATDLEGLIDPRGWGEWEGDFALSTLFYGEYKNTGRGSSTRFRVNWPGFHI 488
           WK+YSRTV + TD++ LIDPRGW EW G +ALSTL+YGE+ NTG G+ T  RVNWPGFH+
Sbjct: 433 WKKYSRTVFLKTDIDELIDPRGWREWSGSYALSTLYYGEFMNTGAGAGTGRRVNWPGFHV 492

Query: 489 FRGDLEAGRFTVGGFLHGRDWIPVTGVPFQAGI 494
            RG+ EA  FTV  F+ G  WIP+TGVPF AG+
Sbjct: 493 LRGEEEASPFTVSRFIQGDSWIPITGVPFSAGV 519

[Close]

BLAST of MS005718 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 389.8 bits (1000), Expect = 3.2e-108
Identity = 216/432 (50.00%), Postives = 278/432 (64.35%), Query Frame = 0


Query: 70  DCTRLYEEADARLAELLFGGNYTAE-DARTWISAAVANHRSCTDGLEEVGAA----ADRK 129
           DC +LY+   +++ E +      ++ DA+TW+S A+ N  +C  G  E+G          
Sbjct: 99  DCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFLELGVTDIVLPLMS 158

Query: 130 NNLTALLAGALRLYEKIA---AVEERNGLKRWEEQKLTKNRGIDLAKWNPATSKADYVVA 189
           NN++ LL   L +  K+       E++G   W +         D      +T K + VVA
Sbjct: 159 NNVSNLLCNTLAI-NKVPFNYTPPEKDGFPSWVKPG-------DRKLLQSSTPKDNAVVA 218

Query: 190 MDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRENVEIGMQLKNVFIVGDGADR 249
            DGSG  +TI +A+ A + SG      R VIYVK GVY EN+EI  + KNV + GDG  +
Sbjct: 219 KDGSGNFKTIKEAIDAASGSG------RFVIYVKQGVYSENLEI--RKKNVMLRGDGIGK 278

Query: 250 TTITGSRNVPDGATTYSSATFAGVSGDGFLARDITFENTAGPEKHQAVALRVNSDLAVVY 309
           T ITGS++V  G TT++SAT A V GDGF+AR ITF NTAG    QAVALR  SDL+V Y
Sbjct: 279 TIITGSKSVGGGTTTFNSATVAAV-GDGFIARGITFRNTAGASNEQAVALRSGSDLSVFY 338

Query: 310 RCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVLQNCEILVRRPMRHQANMITA 369
           +C+ + YQD+L++HS RQFYRDC +YGTVDFIFGN+AAVLQNC I  RRP R + N ITA
Sbjct: 339 QCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRP-RSKTNTITA 398

Query: 370 QGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPWKRYSRTVVMATDLEGLIDPR 429
           QGR DP +NTGI IH SRV AA +   V G  +T+LGRPW++YSRTV M T L+ LIDPR
Sbjct: 399 QGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPR 458

Query: 430 GWGEWEGDFALSTLFYGEYKNTGRGSSTRFRVNWPGFHIFRGDLEAGRFTVGGFLHGRDW 489
           GW EW+G+FAL TLFY E++NTG G+ST  RV WPGF +     EA +FTVG FL G  W
Sbjct: 459 GWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSW 511

Query: 490 IPVTGVPFQAGI 494
           IP + VPF +G+
Sbjct: 519 IP-SSVPFTSGL 511

[Close]

BLAST of MS005718 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 387.9 bits (995), Expect = 1.2e-107
Identity = 205/454 (45.15%), Postives = 274/454 (60.35%), Query Frame = 0


Query: 64  VEPALGDCTRLYEEADARLAELL-----FGGNYTAEDARTWISAAVANHRSCTDGLEE-- 123
           V  A   C  L +++   L   L       G+ +  D  TW+S+A+ NH +CTDG +E  
Sbjct: 137 VRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTNHDTCTDGFDEIE 196

Query: 124 ----------VGAAADRK---NNLTALLAGALRLYEKIAAVEERNGLKRWEEQKL---TK 183
                     +GA  D     +N  A+ AG ++    +  V  R  L   E ++L    K
Sbjct: 197 GQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKLLGTEETEELPNWLK 256

Query: 184 NRGIDLAKWNPATSKADYVVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVY- 243
               +L     +  +AD  V+ DGSGT +TI +A+    +      S R VIYVKAG Y 
Sbjct: 257 REDRELLGTPTSAIQADITVSKDGSGTFKTIAEAI----KKAPEHSSRRFVIYVKAGRYE 316

Query: 244 RENVEIGMQLKNVFIVGDGADRTTITGSRNVPDGATTYSSATFAGVSGDGFLARDITFEN 303
            EN+++G +  N+  +GDG  +T ITG +++ D  TT+ +ATFA  +G GF+ RD+TFEN
Sbjct: 317 EENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFA-ATGAGFIVRDMTFEN 376

Query: 304 TAGPEKHQAVALRVNSDLAVVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAA 363
            AGP KHQAVALRV  D AVVYRC I GYQD+L++HS RQF+R+C IYGTVDFIFGN+A 
Sbjct: 377 YAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFIFGNAAV 436

Query: 364 VLQNCEILVRRPMRHQANMITAQGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGR 423
           +LQ+C I  R+PM  Q   ITAQ R DP +NTGISIH  ++ A P+  A +G + T+LGR
Sbjct: 437 ILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGSYPTYLGR 496

Query: 424 PWKRYSRTVVMATDLEGLIDPRGWGEWEGDFALSTLFYGEYKNTGRGSSTRFRVNWPGFH 483
           PWK YSR V M +D+   IDPRGW EW G FAL +L+YGEY N G GS    RV WPG+H
Sbjct: 497 PWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGIGQRVKWPGYH 556

Query: 484 IFRGDLEAGRFTVGGFLHGRDWIPVTGVPFQAGI 494
           +    +EA +FTV  F+ G  W+P TGV F +G+
Sbjct: 557 VITSTVEASKFTVAQFISGSSWLPSTGVSFFSGL 585

[Close]

BLAST of MS005718 vs. TAIR 10
Match: AT1G23200.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 386.3 bits (991), Expect = 3.6e-107
Identity = 218/464 (46.98%), Postives = 280/464 (60.34%), Query Frame = 0


Query: 67  ALGDCTRLYEEADARL--AELLFGGNYTAEDARTWISAAVANHRSCTDGLEEVGAAADRK 126
           AL DC  LYE+   +L  +   +G   +  D +T +SAA+AN  +C +G  +    +   
Sbjct: 103 ALFDCLELYEDTIDQLNHSRRSYGQYSSPHDRQTSLSAAIANQDTCRNGFRDFKLTSSYS 162

Query: 127 --------NNLTALLAGALRLYEKIA---AVEER---NGLKRWEEQKLTKNRGI------ 186
                    NLT  ++ +L + +  A   AV E+    G  ++ +Q+ +   G       
Sbjct: 163 KYFPVQFHRNLTKSISNSLAVTKAAAEAEAVAEKYPSTGFTKFSKQRSSAGGGSHRRLLL 222

Query: 187 ----DLAKWNP-----------ATSKADYVVAMDGSGTHRTINDAVAALARSGRRRRSER 246
                   W P            T+KAD VVA DGSG + +I  AV A A+    RR++R
Sbjct: 223 FSDEKFPSWFPLSDRKLLEDSKTTAKADLVVAKDGSGHYTSIQQAVNAAAK--LPRRNQR 282

Query: 247 VVIYVKAGVYRENVEIGMQLKNVFIVGDGADRTTITGSRNVPDGATTYSSATFAGVSGDG 306
           +VIYVKAGVYRENV I   +KNV ++GDG D T +TG+RNV DG TT+ SATFA VSG+G
Sbjct: 283 LVIYVKAGVYRENVVIKKSIKNVMVIGDGIDSTIVTGNRNVQDGTTTFRSATFA-VSGNG 342

Query: 307 FLARDITFENTAGPEKHQAVALRVNSDLAVVYRCTIKGYQDSLFLHSLRQFYRDCRIYGT 366
           F+A+ ITFENTAGPEKHQAVALR +SD +V Y C+ KGYQD+L+LHS RQF R+C IYGT
Sbjct: 343 FIAQGITFENTAGPEKHQAVALRSSSDFSVFYACSFKGYQDTLYLHSSRQFLRNCNIYGT 402

Query: 367 VDFIFGNSAAVLQNCEILVRRPMRHQANMITAQGRDDPAENTGISIHGSRVRAAPEFAAV 426
           VDFIFG++ A+LQNC I  R+PM  Q N ITAQ R +P E TG  I  S V  A E    
Sbjct: 403 VDFIFGDATAILQNCNIYARKPMSGQKNTITAQSRKEPDETTGFVIQSSTVATASE---- 462

Query: 427 QGEFRTFLGRPWKRYSRTVVMATDLEGLIDPRGWGEWEGDFALSTLFYGEYKNTGRGSST 486
                T+LGRPW+ +SRTV M  +L  L+ P GW  W G FALSTL+YGEY NTG G+S 
Sbjct: 463 -----TYLGRPWRSHSRTVFMKCNLGALVSPAGWLPWSGSFALSTLYYGEYGNTGAGASV 522

Query: 487 RFRVNWPGFHIFRGDLEAGRFTVGGFLHGRDWIPVTGVPFQAGI 494
             RV WPG+H+ +   EA +FTV  FL G  WI  TGVP   G+
Sbjct: 523 SGRVKWPGYHVIKTVTEAEKFTVENFLDGNYWITATGVPVNDGL 554

[Close]

BLAST of MS005718 vs. TAIR 10
Match: AT1G11580.1 (methylesterase PCR A )

HSP 1 Score: 378.6 bits (971), Expect = 7.5e-105
Identity = 211/449 (46.99%), Postives = 286/449 (63.70%), Query Frame = 0


Query: 60  RRNFVEPALG--DCTRLYEEADARL---AELLFGGNYTAE---DARTWISAAVANHRSCT 119
           R N V    G  DC  + + +  R+    E L GGNY  E   +  TW+S+ + N+ +C 
Sbjct: 113 RSNGVRDKAGFADCEEMMDVSKDRMMSSMEELRGGNYNLESYSNVHTWLSSVLTNYMTCL 172

Query: 120 DGLEEVGAAADR--KNNLTALLAGA-LRLYEKIAAVEERNGLK-----RWEEQKLTKNRG 179
           + + +V   + +  K  L  L++ A + L   ++ +  R+ LK     R+       +R 
Sbjct: 173 ESISDVSVNSKQIVKPQLEDLVSRARVALAIFVSVLPARDDLKMIISNRFPSWLTALDR- 232

Query: 180 IDLAKWNPATSK--ADYVVAMDGSGTHRTINDAVAALARSGRRRRSERVVIYVKAGVYRE 239
             L + +P T K  A+ VVA DG+G  +T+N+AVAA   +     + R VIYVK GVY+E
Sbjct: 233 -KLLESSPKTLKVTANVVVAKDGTGKFKTVNEAVAAAPENS----NTRYVIYVKKGVYKE 292

Query: 240 NVEIGMQLKNVFIVGDGADRTTITGSRNVPDGATTYSSATFAGVSGDGFLARDITFENTA 299
            ++IG + KN+ +VGDG D T ITGS NV DG+TT+ SAT A  +GDGF+A+DI F+NTA
Sbjct: 293 TIDIGKKKKNLMLVGDGKDATIITGSLNVIDGSTTFRSATVA-ANGDGFMAQDIWFQNTA 352

Query: 300 GPEKHQAVALRVNSDLAVVYRCTIKGYQDSLFLHSLRQFYRDCRIYGTVDFIFGNSAAVL 359
           GP KHQAVALRV++D  V+ RC I  YQD+L+ H+LRQFYRD  I GTVDFIFGNSA V 
Sbjct: 353 GPAKHQAVALRVSADQTVINRCRIDAYQDTLYTHTLRQFYRDSYITGTVDFIFGNSAVVF 412

Query: 360 QNCEILVRRPMRHQANMITAQGRDDPAENTGISIHGSRVRAAPEFAAVQGEFRTFLGRPW 419
           QNC+I+ R P   Q NM+TAQGR+D  +NT ISI   ++ A+ + A V+G  +TFLGRPW
Sbjct: 413 QNCDIVARNPGAGQKNMLTAQGREDQNQNTAISIQKCKITASSDLAPVKGSVKTFLGRPW 472

Query: 420 KRYSRTVVMATDLEGLIDPRGWGEWEGDFALSTLFYGEYKNTGRGSSTRFRVNWPGFHIF 479
           K YSRTV+M + ++  IDP GW  W+G+FALSTL+YGEY NTG G+ T  RVNW GF + 
Sbjct: 473 KLYSRTVIMQSFIDNHIDPAGWFPWDGEFALSTLYYGEYANTGPGADTSKRVNWKGFKVI 532

Query: 480 RGDLEAGRFTVGGFLHGRDWIPVTGVPFQ 491
           +   EA +FTV   + G  W+  TGV FQ
Sbjct: 533 KDSKEAEQFTVAKLIQGGLWLKPTGVTFQ 554

The following BLAST results are available for this feature:

BLAST of MS005718 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146909.1	1.8e-273	97.36	pectinesterase [Momordica charantia]	[more]
XP_023540284.1	4.6e-205	75.81	probable pectinesterase/pectinesterase inhibitor 36 [Cucurbita pepo subsp. pepo]	[more]
XP_022974141.1	4.7e-202	74.34	probable pectinesterase/pectinesterase inhibitor 36 [Cucurbita maxima]	[more]
XP_022946489.1	6.2e-202	74.80	probable pectinesterase/pectinesterase inhibitor 36 [Cucurbita moschata]	[more]
KAG6597209.1	1.2e-200	75.78	Methionine aminopeptidase 1A, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of MS005718 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84R10	3.9e-151	53.61	Probable pectinesterase/pectinesterase inhibitor 36 OS=Arabidopsis thaliana OX=3...	[more]
O04887	2.6e-110	45.83	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]
O22149	4.6e-107	50.00	Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3...	[more]
Q9FK05	1.7e-106	45.15	Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3...	[more]
O49298	5.0e-106	46.98	Probable pectinesterase/pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=37...	[more]

BLAST of MS005718 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CZF5	8.5e-274	97.36	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111015995 PE=3 SV=1	[more]
A0A6J1IAJ0	2.3e-202	74.34	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472734 PE=3 SV=1	[more]
A0A6J1G3Y9	3.0e-202	74.80	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111450531 PE=3 SV=1	[more]
A0A5D3D3J9	1.8e-194	70.88	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00376...	[more]
A0A1S3AVG1	1.8e-194	71.08	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483369 PE=3 SV=1	[more]

BLAST of MS005718 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G60730.1	2.8e-152	53.61	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT2G45220.1	3.2e-108	50.00	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G53370.1	1.2e-107	45.15	pectin methylesterase PCR fragment F	[more]
AT1G23200.1	3.6e-107	46.98	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G11580.1	7.5e-105	46.99	methylesterase PCR A	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 15..142 e-value: 3.6E-10 score: 42.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 30..129
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 17..131 e-value: 1.2E-9 score: 38.6
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 177..478 e-value: 4.7E-134 score: 445.8
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 171..493 e-value: 2.3E-111 score: 374.2
None	No IPR available	PANTHER	PTHR31707:SF186	PECTINESTERASE/PECTINESTERASE INHIBITOR 36-RELATED	coord: 8..492
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 8..492
None	No IPR available	CDD	cd15799	PMEI-like_4	coord: 28..138 e-value: 3.13065E-15 score: 70.9019
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 326..335
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 172..492

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS005718.1	MS005718.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity