MS005670 (gene) Bitter gourd (TR) v1

BLAST of MS005670 vs. NCBI nr
Match: XP_023528439.1 (putative invertase inhibitor [Cucurbita pepo subsp. pepo])

HSP 1 Score: 166.0 bits (419), Expect = 3.1e-37
Identity = 103/192 (53.65%), Postives = 125/192 (65.10%), Query Frame = 0

Query: 5   THRLISLALSFFVVFQFQFEGIAGGANDVVIRTCNIAAARDRNNIHVDLCVKALAAAPGS 64
           THR I+LA S FV         +  ANDVV RTC I+AA+D  N+H+D C+++LAAAP S
Sbjct: 6   THRFIALASSLFVALYLN----STVANDVVARTCEISAAKD-PNVHLDFCLRSLAAAPAS 65

Query: 65  ESANLFELGAISIELAREKVSETRVVIESLLQNKKKNKKNKN---------ALRDCEKLY 124
           + A+L+ELGAISIEL     + TR  IE LL   KKNKK KN          L DC +LY
Sbjct: 66  DMADLYELGAISIELIGRNATSTRRYIERLL---KKNKKKKNPSLDSYVRPRLSDCRELY 125

Query: 125 TDAAAALRVATADYLSGRYGDAKTKLIVVMTAVTSCEDGFKE-HGVVSPLAKRNADAFQL 184
           +D  A +R A ADY S RY +   KL  VM AVT+CEDGFKE  G VSPL KRN DAF+L
Sbjct: 126 SDVKATVRDAAADYASKRYEEVNVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNDDAFEL 185

Query: 185 SAVSLSILAMRP 187
           +A++L IL MRP
Sbjct: 186 TAIALCILNMRP 189

BLAST of MS005670 vs. NCBI nr
Match: KAG6582515.1 (hypothetical protein SDJN03_22517, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018899.1 hypothetical protein SDJN02_20772, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 164.1 bits (414), Expect = 1.2e-36
Identity = 101/192 (52.60%), Postives = 124/192 (64.58%), Query Frame = 0

Query: 5   THRLISLALSFFVVFQFQFEGIAGGANDVVIRTCNIAAARDRNNIHVDLCVKALAAAPGS 64
           THR I+LA S FV         +  ANDVV RTC I+AA+D  N+H+D C+++LAAAP S
Sbjct: 6   THRFIALASSLFVALYLN----STVANDVVARTCEISAAKD-PNVHLDFCLRSLAAAPAS 65

Query: 65  ESANLFELGAISIELAREKVSETRVVIESLLQNKKKNKKNKN---------ALRDCEKLY 124
           ++A+L+ELGAISIEL     + TR  IE LL   KKNKK KN          L DC +LY
Sbjct: 66  DTADLYELGAISIELIGRNATSTRRYIERLL---KKNKKKKNPSPDSYLRPPLNDCRELY 125

Query: 125 TDAAAALRVATADYLSGRYGDAKTKLIVVMTAVTSCEDGFKE-HGVVSPLAKRNADAFQL 184
           +D    +R A ADY S RY +   KL  VM A T+CEDGFKE  G VSPL KRN DAF+L
Sbjct: 126 SDVRVTVRDAAADYASKRYEEVNVKLSSVMDAATTCEDGFKEMDGGVSPLTKRNDDAFEL 185

Query: 185 SAVSLSILAMRP 187
           +A++L IL MRP
Sbjct: 186 TAIALCILNMRP 189

BLAST of MS005670 vs. NCBI nr
Match: XP_022924291.1 (putative invertase inhibitor [Cucurbita moschata])

HSP 1 Score: 163.3 bits (412), Expect = 2.0e-36
Identity = 98/189 (51.85%), Postives = 124/189 (65.61%), Query Frame = 0

Query: 5   THRLISLALSFFVVFQFQFEGIAGGANDVVIRTCNIAAARDRNNIHVDLCVKALAAAPGS 64
           THR I+LA S FV         +  ANDVV RTC I+AA+D  N+H+D C+++LAAAP S
Sbjct: 6   THRFIALASSLFVALYL----TSTVANDVVARTCEISAAKD-PNVHLDFCLRSLAAAPAS 65

Query: 65  ESANLFELGAISIELAREKVSETRVVIESLLQNKKKNKKN------KNALRDCEKLYTDA 124
           ++A+L+ELGAISIEL     + TR  IE LL+ KKK K        +  L DC +LY+D 
Sbjct: 66  DTADLYELGAISIELIGRNATSTRRYIERLLKKKKKKKNPSPDSYLRPPLNDCRELYSDV 125

Query: 125 AAALRVATADYLSGRYGDAKTKLIVVMTAVTSCEDGFKE-HGVVSPLAKRNADAFQLSAV 184
              +R A ADY S RY +   KL  +M AVT+CEDGFKE  G VSPL KRN DAF+L+A+
Sbjct: 126 RVTVRDAAADYASKRYEEVNVKLSSIMDAVTTCEDGFKEMDGGVSPLTKRNDDAFELTAI 185

Query: 185 SLSILAMRP 187
           +L IL MRP
Sbjct: 186 ALCILNMRP 189

BLAST of MS005670 vs. NCBI nr
Match: KAA0049108.1 (putative invertase inhibitor [Cucumis melo var. makuwa] >TYK17455.1 putative invertase inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 156.8 bits (395), Expect = 1.9e-34
Identity = 92/187 (49.20%), Postives = 124/187 (66.31%), Query Frame = 0

Query: 5   THRLISLALSFFVVFQFQFEGIAGGANDVVIRTCNIAAARDRNNIHVDLCVKALAAAPGS 64
           THR I+LA SF V+  F+       AND+V RTC  +AARD  N+ +D C+++LAAAPGS
Sbjct: 6   THRCIALAFSFVVLLNFE----PISANDIVSRTCETSAARD-PNVRLDFCLRSLAAAPGS 65

Query: 65  ESANLFELGAISIELAREKVSETRVVIESLLQNKKKNKKNKNALR----DCEKLYTDAAA 124
           ++A+L+ELGAISI L     + T+  IE LL+N+KK   + + +R    DCE+LY+DA  
Sbjct: 66  DTADLYELGAISIRLIGRNATSTQRYIERLLKNEKKKSSSDSYIRPRLSDCEELYSDAVE 125

Query: 125 ALRVATADYLSGRYGDAKTKLIVVMTAVTSCEDGFKE-HGVVSPLAKRNADAFQLSAVSL 184
            +  A A+Y   RY +   KL  VM AVT+CEDGFKE    VSPL KRN D F+L+A++L
Sbjct: 126 TVGEAAAEYGRKRYDEVNVKLSSVMDAVTTCEDGFKEMESRVSPLTKRNGDVFELAAIAL 185

Query: 185 SILAMRP 187
            IL +RP
Sbjct: 186 CILDLRP 187

BLAST of MS005670 vs. NCBI nr
Match: XP_008438273.1 (PREDICTED: putative invertase inhibitor [Cucumis melo])

HSP 1 Score: 156.8 bits (395), Expect = 1.9e-34
Identity = 92/187 (49.20%), Postives = 124/187 (66.31%), Query Frame = 0

Query: 5   THRLISLALSFFVVFQFQFEGIAGGANDVVIRTCNIAAARDRNNIHVDLCVKALAAAPGS 64
           THR I+LA SF V+  F+       AND+V RTC  +AARD  N+ +D C+++LAAAPGS
Sbjct: 6   THRYIALAFSFVVLLNFE----PISANDIVSRTCETSAARD-PNVRLDFCLRSLAAAPGS 65

Query: 65  ESANLFELGAISIELAREKVSETRVVIESLLQNKKKNKKNKNALR----DCEKLYTDAAA 124
           ++A+L+ELGAISI L     + T+  IE LL+N+KK   + + +R    DCE+LY+DA  
Sbjct: 66  DTADLYELGAISIRLIGRNATSTQRYIERLLKNEKKKSSSDSYIRPRLSDCEELYSDAVE 125

Query: 125 ALRVATADYLSGRYGDAKTKLIVVMTAVTSCEDGFKE-HGVVSPLAKRNADAFQLSAVSL 184
            +  A A+Y   RY +   KL  VM AVT+CEDGFKE    VSPL KRN D F+L+A++L
Sbjct: 126 TVGEAAAEYGRKRYDEVNVKLSSVMDAVTTCEDGFKEMESRVSPLTKRNGDVFELAAIAL 185

Query: 185 SILAMRP 187
            IL +RP
Sbjct: 186 CILDLRP 187

BLAST of MS005670 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 6.7e-19
Identity = 62/151 (41.06%), Postives = 88/151 (58.28%), Query Frame = 0

Query: 32  DVVIRTCNIAAARDRNNIHVDLCVKALAAAPGSESANLFELGAISIELAREKVSETRVVI 91
           D+V  TC   A R   N++ D CVK+L A P S SA+L  LG IS  LA ++ S+ +  I
Sbjct: 16  DIVQGTCKKVAQRS-PNVNYDFCVKSLGADPKSHSADLQGLGVISANLAIQQGSKIQTFI 75

Query: 92  ESLLQNKKKNKKNKNALRDCEKLYTDAAAALRVATADYLSGRYGDAKTKLIVVMTAVTSC 151
             +L++ K +   K  L DC  LY DA ++++ A AD+ S  Y  A  K+   +    +C
Sbjct: 76  GRILKS-KVDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDSVTC 135

Query: 152 EDGFKE-HGVVSPLAKRNADAFQLSAVSLSI 182
           EDGFKE  G+ SP+ K N D  QL+A+SL+I
Sbjct: 136 EDGFKEKKGIASPVTKENKDYVQLTAISLAI 164

BLAST of MS005670 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 8.7e-19
Identity = 62/151 (41.06%), Postives = 88/151 (58.28%), Query Frame = 0

Query: 32  DVVIRTCNIAAARDRNNIHVDLCVKALAAAPGSESANLFELGAISIELAREKVSETRVVI 91
           D+V  TC   A R   N++ D CVK+L A P S +A+L  LG IS  LA +  S+ +  I
Sbjct: 25  DIVQGTCKKVAQRS-PNVNYDFCVKSLGADPKSHTADLQGLGVISANLAIQHGSKIQTFI 84

Query: 92  ESLLQNKKKNKKNKNALRDCEKLYTDAAAALRVATADYLSGRYGDAKTKLIVVMTAVTSC 151
             +L++ K +   K  L DC  LY DA ++++ A AD+ S  Y  A  K+   +    +C
Sbjct: 85  GRILKS-KVDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDSVTC 144

Query: 152 EDGFKE-HGVVSPLAKRNADAFQLSAVSLSI 182
           EDGFKE  G+VSP+ K N D  QL+A+SL+I
Sbjct: 145 EDGFKEKKGIVSPVTKENKDYVQLTAISLAI 173

BLAST of MS005670 vs. ExPASy TrEMBL
Match: A0A6J1E955 (putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111431821 PE=4 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 9.7e-37
Identity = 98/189 (51.85%), Postives = 124/189 (65.61%), Query Frame = 0

Query: 5   THRLISLALSFFVVFQFQFEGIAGGANDVVIRTCNIAAARDRNNIHVDLCVKALAAAPGS 64
           THR I+LA S FV         +  ANDVV RTC I+AA+D  N+H+D C+++LAAAP S
Sbjct: 6   THRFIALASSLFVALYL----TSTVANDVVARTCEISAAKD-PNVHLDFCLRSLAAAPAS 65

Query: 65  ESANLFELGAISIELAREKVSETRVVIESLLQNKKKNKKN------KNALRDCEKLYTDA 124
           ++A+L+ELGAISIEL     + TR  IE LL+ KKK K        +  L DC +LY+D 
Sbjct: 66  DTADLYELGAISIELIGRNATSTRRYIERLLKKKKKKKNPSPDSYLRPPLNDCRELYSDV 125

Query: 125 AAALRVATADYLSGRYGDAKTKLIVVMTAVTSCEDGFKE-HGVVSPLAKRNADAFQLSAV 184
              +R A ADY S RY +   KL  +M AVT+CEDGFKE  G VSPL KRN DAF+L+A+
Sbjct: 126 RVTVRDAAADYASKRYEEVNVKLSSIMDAVTTCEDGFKEMDGGVSPLTKRNDDAFELTAI 185

Query: 185 SLSILAMRP 187
           +L IL MRP
Sbjct: 186 ALCILNMRP 189

BLAST of MS005670 vs. ExPASy TrEMBL
Match: A0A5A7U1K7 (Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003190 PE=4 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 9.0e-35
Identity = 92/187 (49.20%), Postives = 124/187 (66.31%), Query Frame = 0

Query: 5   THRLISLALSFFVVFQFQFEGIAGGANDVVIRTCNIAAARDRNNIHVDLCVKALAAAPGS 64
           THR I+LA SF V+  F+       AND+V RTC  +AARD  N+ +D C+++LAAAPGS
Sbjct: 6   THRCIALAFSFVVLLNFE----PISANDIVSRTCETSAARD-PNVRLDFCLRSLAAAPGS 65

Query: 65  ESANLFELGAISIELAREKVSETRVVIESLLQNKKKNKKNKNALR----DCEKLYTDAAA 124
           ++A+L+ELGAISI L     + T+  IE LL+N+KK   + + +R    DCE+LY+DA  
Sbjct: 66  DTADLYELGAISIRLIGRNATSTQRYIERLLKNEKKKSSSDSYIRPRLSDCEELYSDAVE 125

Query: 125 ALRVATADYLSGRYGDAKTKLIVVMTAVTSCEDGFKE-HGVVSPLAKRNADAFQLSAVSL 184
            +  A A+Y   RY +   KL  VM AVT+CEDGFKE    VSPL KRN D F+L+A++L
Sbjct: 126 TVGEAAAEYGRKRYDEVNVKLSSVMDAVTTCEDGFKEMESRVSPLTKRNGDVFELAAIAL 185

Query: 185 SILAMRP 187
            IL +RP
Sbjct: 186 CILDLRP 187

BLAST of MS005670 vs. ExPASy TrEMBL
Match: A0A1S3AVZ7 (putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103483434 PE=4 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 9.0e-35
Identity = 92/187 (49.20%), Postives = 124/187 (66.31%), Query Frame = 0

Query: 5   THRLISLALSFFVVFQFQFEGIAGGANDVVIRTCNIAAARDRNNIHVDLCVKALAAAPGS 64
           THR I+LA SF V+  F+       AND+V RTC  +AARD  N+ +D C+++LAAAPGS
Sbjct: 6   THRYIALAFSFVVLLNFE----PISANDIVSRTCETSAARD-PNVRLDFCLRSLAAAPGS 65

Query: 65  ESANLFELGAISIELAREKVSETRVVIESLLQNKKKNKKNKNALR----DCEKLYTDAAA 124
           ++A+L+ELGAISI L     + T+  IE LL+N+KK   + + +R    DCE+LY+DA  
Sbjct: 66  DTADLYELGAISIRLIGRNATSTQRYIERLLKNEKKKSSSDSYIRPRLSDCEELYSDAVE 125

Query: 125 ALRVATADYLSGRYGDAKTKLIVVMTAVTSCEDGFKE-HGVVSPLAKRNADAFQLSAVSL 184
            +  A A+Y   RY +   KL  VM AVT+CEDGFKE    VSPL KRN D F+L+A++L
Sbjct: 126 TVGEAAAEYGRKRYDEVNVKLSSVMDAVTTCEDGFKEMESRVSPLTKRNGDVFELAAIAL 185

Query: 185 SILAMRP 187
            IL +RP
Sbjct: 186 CILDLRP 187

BLAST of MS005670 vs. ExPASy TrEMBL
Match: A0A0A0L7T4 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G128870 PE=4 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 3.4e-34
Identity = 92/184 (50.00%), Postives = 125/184 (67.93%), Query Frame = 0

Query: 6   HRLISLALSFFVVFQFQFEGIAGGANDVVIRTCNIAAARDRNNIHVDLCVKALAAAPGSE 65
           HR I+LALSFFV+F  +    +  AND + R C ++AA D  N+ +D C+++LAAAPGS+
Sbjct: 7   HRFIALALSFFVLFNLR----SISANDTLSRACELSAASD-PNVRLDFCLQSLAAAPGSD 66

Query: 66  SANLFELGAISIELAREKVSETRVVIESLLQNKKKNKKN--KNALRDCEKLYTDAAAALR 125
           +A+L+ELGA+SI+L     + TR  IE LL+N+KK+     +  L DCE+LY DA  A+ 
Sbjct: 67  TADLYELGALSIKLIAWNATSTRRYIERLLKNEKKSPDPYVRPRLSDCEELYIDAIKAVG 126

Query: 126 VATADYLSGRYGDAKTKLIVVMTAVTSCEDGFKE-HGVVSPLAKRNADAFQLSAVSLSIL 185
            A  +Y   RY +   KL  VM AVT+CEDGFKE  G VSPL KRN D F+L+A++LSIL
Sbjct: 127 DAAFEYGRNRYEEVNVKLSSVMDAVTTCEDGFKEMEGRVSPLTKRNGDVFELTAIALSIL 185

Query: 186 AMRP 187
            +RP
Sbjct: 187 NLRP 185

BLAST of MS005670 vs. ExPASy TrEMBL
Match: A0A6J1ITK8 (putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111479799 PE=4 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 4.5e-34
Identity = 93/167 (55.69%), Postives = 112/167 (67.07%), Query Frame = 0

Query: 30  ANDVVIRTCNIAAARDRNNIHVDLCVKALAAAPGSESANLFELGAISIELAREKVSETRV 89
           ANDVV R C I AA+D  N+H+D C+++LAAAP S+ A+L+ELGAISIEL     + TR 
Sbjct: 25  ANDVVARNCEIFAAKD-PNVHLDFCLRSLAAAPASDMADLYELGAISIELIGRNATSTRR 84

Query: 90  VIESLLQNKKKNKKNKN---------ALRDCEKLYTDAAAALRVATADYLSGRYGDAKTK 149
            IE LL   KKNKK KN          L DC +LY+D  A +R A ADY S RY +   K
Sbjct: 85  YIERLL---KKNKKKKNPSLDSYVRPRLSDCRELYSDVKATVRDAAADYASKRYEEVNVK 144

Query: 150 LIVVMTAVTSCEDGFKE-HGVVSPLAKRNADAFQLSAVSLSILAMRP 187
           L  VM AVT+CEDGFKE  G VSPL KRN DAF+L+A++L IL MRP
Sbjct: 145 LSSVMDAVTTCEDGFKEMDGGVSPLTKRNDDAFELTAIALCILNMRP 187

BLAST of MS005670 vs. TAIR 10
Match: AT5G46940.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 73.2 bits (178), Expect = 2.5e-13
Identity = 55/173 (31.79%), Postives = 82/173 (47.40%), Query Frame = 0

Query: 13  LSFFVVFQFQFEGIAGGANDVVIRTCNIAAARDRNNIHVDLCVKALAAAPGSESA-NLFE 72
           L + V F     G+A G    +IR     A         +LCV +L   P +++A +L  
Sbjct: 4   LLYLVTFFVLSNGLANG--QTLIRNSCKKATATSPKFKYNLCVTSLETNPQAKTAKDLAG 63

Query: 73  LGAISIELAREKVSETRVVIESLLQNKKKNKKNKNALRDCEKLYTDAAAALRVATADYLS 132
           L   S + A  K +  +  ++ +++ KK NK     LRDC +LYTDA  +L  A A   S
Sbjct: 64  LVMASTKNAVTKATTLKGTVDKIIKGKKVNKMTAMPLRDCLQLYTDAIGSLNEALAGVKS 123

Query: 133 GRYGDAKTKLIVVMTAVTSCEDGFKEHGVVSPLAKRNADAFQLSAVSLSILAM 185
             Y   KT L   M   ++CE GFKE    SP+ K N + +Q+  + L+   M
Sbjct: 124 RNYPTVKTVLSAAMDTPSTCETGFKERKAPSPVTKENDNLYQMILIPLAFTNM 174

BLAST of MS005670 vs. TAIR 10
Match: AT3G36659.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 42.7 bits (99), Expect = 3.7e-04
Identity = 22/56 (39.29%), Postives = 28/56 (50.00%), Query Frame = 0

Query: 110 DCEKLYTDAAAALRVATADYLSGRYGDAKTKLIVVMTAVTSCEDGFKEHGVVSPLA 166
           DC++LY DA   L  A     S       T L   MT  ++C DGF+E G  +PLA
Sbjct: 188 DCQELYDDAVVNLEDAVNAVKSSDIATVNTNLSAAMTDYSTCNDGFEESGEPNPLA 243