MS005647 (gene) Bitter gourd (TR) v1

Overview


Name	MS005647
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Werner Syndrome-like exonuclease
Location	scaffold254: 532715 .. 535077 (+)
RNA-Seq Expression	MS005647
Synteny	MS005647

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAATTCCCCGGTTGCAGCAGGAATGCCGGCGCTTCGCCGCCGCTCTCCGTCTCTTCCTCCTCCTTCTCGTCGCGTTCGTTCTCTGTCTCGGAGTTCGATCAGCCTTTTACAGAGCAACAACTTCAGGAAATCGATGCCATCGAAGCTATGTATCAATCCACCAGCGGCAAAAGACGACGAGGAAGTTCCACAGCCGACGAAAACGAGGCCTCGTGTGTTTCCCCCAACGCCGGCCGTCGATTGCCCCATTCCATTCTTTCTCTCCAGCTTCCTTATTCTTCTCCTCTATCGCCCCGTCGAGGTATTTTTTTTCTAAATTCTATTTAACAAATTGGTTGCAGCAGATGATGCAGATTTGGGAAAGATTGCTTGGGGCTTTCAAACTACATTTTGAGGATCTACGGCTTATTTTGAGCTGAAATTGAAATTATGATTACATTGTTTATTGGTTTCATTTGCAGTAAATTCGAAGATGAGATTTCCTGCATTGAATTTTGATGGCCGAATTATTTACAGTAGAACTGCGAGTGAGGTAGATAGAGCGTCCAGGGAGTTAGCCAAGAAAATCAGCTCCACGAGGAAAGGAACGGATCAAATTACTATCGGATTCGACATCGAGTGGAAACCCACATTTAGAAGAGGTGAGGGAACTTTGAAGTTTTATTTCTTGATCCAAAATATTTTTGTTATTCTCACCCGAATATAATGGCAGTTCTGTTTCTCATGGGAATCAAAATCGTAGTGTCTTTAAATTAACAAGAAACACTATTTTGCTTTCCTCCTTAATTTGTCCGTCTGTTCGTTGGGATTTTGTTATATTGAAGTTTTTCCCTAAGAAAATTTATGGCGGACGATAGAATTAATGTATGAAAATATTCATTTTACCATTGACTATATTGAATTACACACACCCATATTAGATTGAGCTTGTTGCTATGAATAGAGGAAGTCAAAGCTCAAATTAGTTCAAGCACAGTTATACAGCTAACTGTTAAGTTGTGAACTCTCTCAACCACACTTCGAATTAGTCAAGTTTCAGAATCTTAGTTGGGCCAATTAGTTGATTTAACACTTATGTTCTATAATGATTGGCTCGCACAACTTATTGTTAGCAGATGGTTAAATCGGTTAAGAAGTCAGTCCTTCTCAGAGAGTTAGACAGTGAATACTTATAACTTGTTTTTGTAATGTTCCCGTTTGTTCTTTCTGTTCTTCATCTTTAATAAAAAAAATAGAATGACATGACTTTAACACCTATAAATTCAAGAATGATAAAACAATATTTTAAAAATGAGAAACAACAAACCTAAATGGTTTGTCAAATGCTTGGAAACAAGAAACAAGAAATGAGAATATTACCAAACGGACCTTTAATTTCCCGATTCTTTTATATCTTTCTCTTTCCCTTCATATAGTTTGTTGTTTCATCTGGCTCTTGCGTAGACTTAAATCTTAGCTGGGTTGGTGGCTGTTAACTCGACCGAACTGGTGCTAATTACTTCTTCTATCATTCTTACAGCCTATTTAATTGGTCTTGGTCTCTTGGATTCCATAATGTCTCTGTCTTATTGACTCTTTTGTGATGAATTTTATATGCAGGTGTTCCACCCGGAAAGACAGCAGTCATGCAACTATGCTTGGACAATTCTCAATGCCATGTGATGCATATCATTCATTCGGGAATAACCCCAAGTTTGAAGACTCTTCTTGAGGATGATACACTCAACAAGGTTTGAAACATTACCATAACTGCTCTTGTTATTTAGTTTTCCTGTACTATTTATATGTTTATAGCCTATTTCGATCGATCATGCTAAAAAACTTCAGGCTGGAGTTGGCATTGCCAATGATGCTGTGAAAGTTTTTAAAGATTATAATGTGTCTATCAAAGCAGTGAGCGAGATTTCAGATCTAGCTAATCAAAAGCTTGCTGGAGTTCCCCAAAAATGGGGTCTTGAGTCTCTAACTAAGGCGCTTGTATCAAAAGAGGTTGTTTATTTCTGTTTATGTTCAAGTATTTAAAAGATATTTGCAATTTTCAAATCAAAGTCTCATGGTAACCAAAATTACTGAACTTTGTAGCTTCAAAAGCCCAACAAAATCAGACTTGGAAACTGGGAGGTCGCTGTGCTATCAAAGCAGCAACTGCAGTATGCTGCCACAGACGCATTTGCTTCTTGGTACTTACATGAGGTATTATCAAATGGCCCTTTCTCTCTTCATGTGGTAATTGTTGTTTTAAGATCTCATTCATAAACAGAGGATTTCATCATTATAATAATTTCGACCTTTAACAATATTCACAGATCTTAAAAGGATTTCCGCATGCTCAAAAAGTAGAAGATTCAGAGAGAGATATG

mRNA sequence

ATGGAATTCCCCGGTTGCAGCAGGAATGCCGGCGCTTCGCCGCCGCTCTCCGTCTCTTCCTCCTCCTTCTCGTCGCGTTCGTTCTCTGTCTCGGAGTTCGATCAGCCTTTTACAGAGCAACAACTTCAGGAAATCGATGCCATCGAAGCTATGTATCAATCCACCAGCGGCAAAAGACGACGAGGAAGTTCCACAGCCGACGAAAACGAGGCCTCGTGTGTTTCCCCCAACGCCGGCCGTCGATTGCCCCATTCCATTCTTTCTCTCCAGCTTCCTTATTCTTCTCCTCTATCGCCCCGTCGAGTAAATTCGAAGATGAGATTTCCTGCATTGAATTTTGATGGCCGAATTATTTACAGTAGAACTGCGAGTGAGGTAGATAGAGCGTCCAGGGAGTTAGCCAAGAAAATCAGCTCCACGAGGAAAGGAACGGATCAAATTACTATCGGATTCGACATCGAGTGGAAACCCACATTTAGAAGAGGTGTTCCACCCGGAAAGACAGCAGTCATGCAACTATGCTTGGACAATTCTCAATGCCATGTGATGCATATCATTCATTCGGGAATAACCCCAAGTTTGAAGACTCTTCTTGAGGATGATACACTCAACAAGGCTGGAGTTGGCATTGCCAATGATGCTGTGAAAGTTTTTAAAGATTATAATGTGTCTATCAAAGCAGTGAGCGAGATTTCAGATCTAGCTAATCAAAAGCTTGCTGGAGTTCCCCAAAAATGGGGTCTTGAGTCTCTAACTAAGGCGCTTGTATCAAAAGAGCTTCAAAAGCCCAACAAAATCAGACTTGGAAACTGGGAGGTCGCTGTGCTATCAAAGCAGCAACTGCAGTATGCTGCCACAGACGCATTTGCTTCTTGGTACTTACATGAGATCTTAAAAGGATTTCCGCATGCTCAAAAAGTAGAAGATTCAGAGAGAGATATG

Coding sequence (CDS)

ATGGAATTCCCCGGTTGCAGCAGGAATGCCGGCGCTTCGCCGCCGCTCTCCGTCTCTTCCTCCTCCTTCTCGTCGCGTTCGTTCTCTGTCTCGGAGTTCGATCAGCCTTTTACAGAGCAACAACTTCAGGAAATCGATGCCATCGAAGCTATGTATCAATCCACCAGCGGCAAAAGACGACGAGGAAGTTCCACAGCCGACGAAAACGAGGCCTCGTGTGTTTCCCCCAACGCCGGCCGTCGATTGCCCCATTCCATTCTTTCTCTCCAGCTTCCTTATTCTTCTCCTCTATCGCCCCGTCGAGTAAATTCGAAGATGAGATTTCCTGCATTGAATTTTGATGGCCGAATTATTTACAGTAGAACTGCGAGTGAGGTAGATAGAGCGTCCAGGGAGTTAGCCAAGAAAATCAGCTCCACGAGGAAAGGAACGGATCAAATTACTATCGGATTCGACATCGAGTGGAAACCCACATTTAGAAGAGGTGTTCCACCCGGAAAGACAGCAGTCATGCAACTATGCTTGGACAATTCTCAATGCCATGTGATGCATATCATTCATTCGGGAATAACCCCAAGTTTGAAGACTCTTCTTGAGGATGATACACTCAACAAGGCTGGAGTTGGCATTGCCAATGATGCTGTGAAAGTTTTTAAAGATTATAATGTGTCTATCAAAGCAGTGAGCGAGATTTCAGATCTAGCTAATCAAAAGCTTGCTGGAGTTCCCCAAAAATGGGGTCTTGAGTCTCTAACTAAGGCGCTTGTATCAAAAGAGCTTCAAAAGCCCAACAAAATCAGACTTGGAAACTGGGAGGTCGCTGTGCTATCAAAGCAGCAACTGCAGTATGCTGCCACAGACGCATTTGCTTCTTGGTACTTACATGAGATCTTAAAAGGATTTCCGCATGCTCAAAAAGTAGAAGATTCAGAGAGAGATATG

Protein sequence

MEFPGCSRNAGASPPLSVSSSSFSSRSFSVSEFDQPFTEQQLQEIDAIEAMYQSTSGKRRRGSSTADENEASCVSPNAGRRLPHSILSLQLPYSSPLSPRRVNSKMRFPALNFDGRIIYSRTASEVDRASRELAKKISSTRKGTDQITIGFDIEWKPTFRRGVPPGKTAVMQLCLDNSQCHVMHIIHSGITPSLKTLLEDDTLNKAGVGIANDAVKVFKDYNVSIKAVSEISDLANQKLAGVPQKWGLESLTKALVSKELQKPNKIRLGNWEVAVLSKQQLQYAATDAFASWYLHEILKGFPHAQKVEDSERDM

Homology

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BLAST of MS005647 vs. NCBI nr
Match: XP_022147109.1 (Werner Syndrome-like exonuclease [Momordica charantia])

HSP 1 Score: 609.4 bits (1570), Expect = 1.8e-170
Identity = 311/314 (99.04%), Postives = 312/314 (99.36%), Query Frame = 0


Query: 1   MEFPGCSRNAGASPPLSVSSSSFSSRSFSVSEFDQPFTEQQLQEIDAIEAMYQSTSGKRR 60
           MEFPGCSRNAGASPPLSVSSSSFSSRSFSVSEFDQPFTEQQLQEIDAIEAMYQSTSGKRR
Sbjct: 1   MEFPGCSRNAGASPPLSVSSSSFSSRSFSVSEFDQPFTEQQLQEIDAIEAMYQSTSGKRR 60

Query: 61  RGSSTADENEASCVSPNAGRRLPHSILSLQLPYSSPLSPRRVNSKMRFPALNFDGRIIYS 120
           RGSSTADENEASCVSPNAGRRLPHSILSLQLPYSSPLSPRRVNSKMRFPALNFDGRIIYS
Sbjct: 61  RGSSTADENEASCVSPNAGRRLPHSILSLQLPYSSPLSPRRVNSKMRFPALNFDGRIIYS 120

Query: 121 RTASEVDRASRELAKKISSTRKGTDQITIGFDIEWKPTFRRGVPPGKTAVMQLCLDNSQC 180
            TASEVDRASRELAKKISSTRKGTDQITIGFDIEWKPTFRRGVPPGKTAVMQLCLDNSQC
Sbjct: 121 TTASEVDRASRELAKKISSTRKGTDQITIGFDIEWKPTFRRGVPPGKTAVMQLCLDNSQC 180

Query: 181 HVMHIIHSGITPSLKTLLEDDTLNKAGVGIANDAVKVFKDYNVSIKAVSEISDLANQKLA 240
           HVMHIIHSGITPSLKTLLEDDTL+KAGVGIANDAVKVFKDYNVSIKAVSEISDLANQKLA
Sbjct: 181 HVMHIIHSGITPSLKTLLEDDTLSKAGVGIANDAVKVFKDYNVSIKAVSEISDLANQKLA 240

Query: 241 GVPQKWGLESLTKALVSKELQKPNKIRLGNWEVAVLSKQQLQYAATDAFASWYLHEILKG 300
           GVPQKWGL SLTKALVSKELQKPNKIRLGNWEVAVLSKQQLQYAATDAFASWYLHEILKG
Sbjct: 241 GVPQKWGLGSLTKALVSKELQKPNKIRLGNWEVAVLSKQQLQYAATDAFASWYLHEILKG 300

Query: 301 FPHAQKVEDSERDM 315
           FPHAQKVEDSERDM
Sbjct: 301 FPHAQKVEDSERDM 314

[Close]

BLAST of MS005647 vs. NCBI nr
Match: XP_038897657.1 (Werner Syndrome-like exonuclease [Benincasa hispida])

HSP 1 Score: 487.3 bits (1253), Expect = 1.0e-133
Identity = 258/312 (82.69%), Postives = 271/312 (86.86%), Query Frame = 0


Query: 1   MEFPGCSRNAG-ASPPLSVSSSSFSSRSFSVSEFDQPFTEQQLQEIDAIEAMYQSTSGKR 60
           MEF  CS N G +S  LSV SSS SS SFS SEFDQPFTEQQLQEI+AIEA YQSTS K+
Sbjct: 1   MEF-HCSSNGGHSSTKLSVCSSS-SSWSFSASEFDQPFTEQQLQEIEAIEAAYQSTSAKK 60

Query: 61  RRGSSTADENEASCVSPNAGRRLPHSILSLQLPYSSPLSPRRVNSKMRFPALNFDGRIIY 120
           RR +ST DENE SCVSP+ GRRLP SILS Q P+SSPLSP RVNSKMRFPALNF GRIIY
Sbjct: 61  RRPNSTPDENEVSCVSPDTGRRLPRSILSHQSPHSSPLSPCRVNSKMRFPALNFGGRIIY 120

Query: 121 SRTASEVDRASRELAKKISSTRKGTDQITIGFDIEWKPTFRRGVPPGKTAVMQLCLDNSQ 180
           SRT SEVDRASRELAKKI+S RKG DQITIGFDIEWKPTFRRGVPPGK AVMQLCLDNSQ
Sbjct: 121 SRTWSEVDRASRELAKKINSMRKGMDQITIGFDIEWKPTFRRGVPPGKAAVMQLCLDNSQ 180

Query: 181 CHVMHIIHSGITPSLKTLLEDDTLNKAGVGIANDAVKVFKDYNVSIKAVSEISDLANQKL 240
           CHVMHIIHSGI  SL+ LLEDDTL KAGVGI NDA KV KDYNVSIKAV EISD+ANQKL
Sbjct: 181 CHVMHIIHSGIPQSLQALLEDDTLTKAGVGIVNDATKVLKDYNVSIKAVDEISDIANQKL 240

Query: 241 AGVPQKWGLESLTKALVSKELQKPNKIRLGNWEVAVLSKQQLQYAATDAFASWYLHEILK 300
           AG P+KW L SLT+ L+SKELQKPNKIRLGNWEVAVLS +QLQYAATDAFASWYLHEILK
Sbjct: 241 AGFPRKWSLGSLTETLISKELQKPNKIRLGNWEVAVLSTEQLQYAATDAFASWYLHEILK 300

Query: 301 GFPHAQKVEDSE 312
           GFPHAQKV D E
Sbjct: 301 GFPHAQKVADPE 310

[Close]

BLAST of MS005647 vs. NCBI nr
Match: KAG7028742.1 (Werner Syndrome-like exonuclease [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 481.1 bits (1237), Expect = 7.3e-132
Identity = 251/313 (80.19%), Postives = 270/313 (86.26%), Query Frame = 0


Query: 1   MEFPGCSRNAGASPPLSVSSSSFSSRSFSVSEFDQPFTEQQLQEIDAIEAMYQSTSGKRR 60
           MEFP  S  AGAS  LSV SS  SS SFS+SEFDQPFTEQQLQ I+AIEA YQ+T+ KRR
Sbjct: 1   MEFPCSSNRAGASTELSVCSSPSSSYSFSLSEFDQPFTEQQLQAIEAIEAAYQATTAKRR 60

Query: 61  RGSSTADENEASCVSPNAGRRLPHSILSLQLPYSSPLSPRRVNSKMRFPALNFDGRIIYS 120
           R      ENE SCVSP++GRRLP SI SLQ P+SSPLSP RV SKMRFPAL FDGRI+YS
Sbjct: 61  R------ENEVSCVSPDSGRRLPRSISSLQSPHSSPLSPCRVTSKMRFPALKFDGRIVYS 120

Query: 121 RTASEVDRASRELAKKISSTRKGTDQITIGFDIEWKPTFRRGVPPGKTAVMQLCLDNSQC 180
           RTASEVDRASRELAKKI+S RKG DQITIGFDIEWKPTFRRGVPPGK AVMQLCLD+SQC
Sbjct: 121 RTASEVDRASRELAKKINSMRKGMDQITIGFDIEWKPTFRRGVPPGKAAVMQLCLDHSQC 180

Query: 181 HVMHIIHSGITPSLKTLLEDDTLNKAGVGIANDAVKVFKDYNVSIKAVSEISDLANQKLA 240
           HVMHIIHSGI  SL+ LLEDDTL+KAGVGI NDA+KVFKDYNVSIKAV EIS+LANQKLA
Sbjct: 181 HVMHIIHSGIPRSLQALLEDDTLSKAGVGIVNDAMKVFKDYNVSIKAVDEISNLANQKLA 240

Query: 241 GVPQKWGLESLTKALVSKELQKPNKIRLGNWEVAVLSKQQLQYAATDAFASWYLHEILKG 300
           GVP+KW L SLT+AL+SKEL KP+KIRLGNWEVAVLSK+QL YAATDAFASWYLHEILKG
Sbjct: 241 GVPKKWSLGSLTEALISKELPKPSKIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKG 300

Query: 301 FPHAQKVEDSERD 314
           FP  QK  DSE D
Sbjct: 301 FPDPQKAADSEGD 307

[Close]

BLAST of MS005647 vs. NCBI nr
Match: KAG6597271.1 (Werner Syndrome-like exonuclease, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 480.7 bits (1236), Expect = 9.5e-132
Identity = 250/313 (79.87%), Postives = 270/313 (86.26%), Query Frame = 0


Query: 1   MEFPGCSRNAGASPPLSVSSSSFSSRSFSVSEFDQPFTEQQLQEIDAIEAMYQSTSGKRR 60
           MEFP  S  AGAS  LSV SS  SS SFS+SEFDQPFTEQQLQ I+AIEA YQ+T+ KRR
Sbjct: 1   MEFPCSSNRAGASTELSVCSSPSSSYSFSLSEFDQPFTEQQLQAIEAIEAAYQATTAKRR 60

Query: 61  RGSSTADENEASCVSPNAGRRLPHSILSLQLPYSSPLSPRRVNSKMRFPALNFDGRIIYS 120
           R      ENE SCVSP++GRRLP S+ SLQ P+SSPLSP RV SKMRFPAL FDGRI+YS
Sbjct: 61  R------ENEVSCVSPDSGRRLPRSVSSLQSPHSSPLSPCRVTSKMRFPALKFDGRIVYS 120

Query: 121 RTASEVDRASRELAKKISSTRKGTDQITIGFDIEWKPTFRRGVPPGKTAVMQLCLDNSQC 180
           RTASEVDRASRELAKKI+S RKG DQITIGFDIEWKPTFRRGVPPGK AVMQLCLD+SQC
Sbjct: 121 RTASEVDRASRELAKKINSMRKGMDQITIGFDIEWKPTFRRGVPPGKAAVMQLCLDHSQC 180

Query: 181 HVMHIIHSGITPSLKTLLEDDTLNKAGVGIANDAVKVFKDYNVSIKAVSEISDLANQKLA 240
           HVMHIIHSGI  SL+ LLEDDTL+KAGVGI NDA+KVFKDYNVSIKAV EIS+LANQKLA
Sbjct: 181 HVMHIIHSGIPRSLQALLEDDTLSKAGVGIVNDAMKVFKDYNVSIKAVDEISNLANQKLA 240

Query: 241 GVPQKWGLESLTKALVSKELQKPNKIRLGNWEVAVLSKQQLQYAATDAFASWYLHEILKG 300
           GVP+KW L SLT+AL+SKEL KP+KIRLGNWEVAVLSK+QL YAATDAFASWYLHEILKG
Sbjct: 241 GVPKKWSLGSLTEALISKELPKPSKIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKG 300

Query: 301 FPHAQKVEDSERD 314
           FP  QK  DSE D
Sbjct: 301 FPDPQKAADSEGD 307

[Close]

BLAST of MS005647 vs. NCBI nr
Match: XP_008438323.1 (PREDICTED: Werner Syndrome-like exonuclease [Cucumis melo])

HSP 1 Score: 478.0 bits (1229), Expect = 6.2e-131
Identity = 242/311 (77.81%), Postives = 270/311 (86.82%), Query Frame = 0


Query: 1   MEFPGCSRNAGASPPLSVSSSSFSSRSFSVSEFDQPFTEQQLQEIDAIEAMYQSTSGKRR 60
           MEFPG S +  +S  LS  SSSF S SFS SEFDQPFTEQQLQ I+AIEA YQSTS K+R
Sbjct: 1   MEFPGSSNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60

Query: 61  RGSSTADENEASCVSPNAGRRLPHSILSLQLPYSSPLSPRRVNSKMRFPALNFDGRIIYS 120
           R +ST DEN  SCVSPN GRRLP SI S Q P+ SPLS  RVNSKMRFPAL++ GRIIYS
Sbjct: 61  RPNSTLDENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYS 120

Query: 121 RTASEVDRASRELAKKISSTRKGTDQITIGFDIEWKPTFRRGVPPGKTAVMQLCLDNSQC 180
           RT SEVDRASRELAKKI+STRKG +QITIGFDIEW+P+F+RGVPPGK AVMQLCL+NS+C
Sbjct: 121 RTVSEVDRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180

Query: 181 HVMHIIHSGITPSLKTLLEDDTLNKAGVGIANDAVKVFKDYNVSIKAVSEISDLANQKLA 240
           HVMHIIHSGI  SL+ LLEDDTLNKAGVGIA+DA KVFKDYNVS+K + EISDLANQKLA
Sbjct: 181 HVMHIIHSGIPQSLQALLEDDTLNKAGVGIASDASKVFKDYNVSMKQLDEISDLANQKLA 240

Query: 241 GVPQKWGLESLTKALVSKELQKPNKIRLGNWEVAVLSKQQLQYAATDAFASWYLHEILKG 300
           GVP+KWGL +LT+ L+SKEL+KP++IRLGNWEVAVLSK+QL YAATDAFASWYLHEILKG
Sbjct: 241 GVPKKWGLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKG 300

Query: 301 FPHAQKVEDSE 312
           FPH +KV DSE
Sbjct: 301 FPHVEKVADSE 311

[Close]

BLAST of MS005647 vs. ExPASy Swiss-Prot
Match: Q84LH3 (Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 5.3e-69
Identity = 141/281 (50.18%), Postives = 185/281 (65.84%), Query Frame = 0


Query: 34  DQPFTEQQLQEIDAIEAMYQSTSGKRRRGSST----------ADENEASCVSPNAGRRLP 93
           D  FTE++L  IDAIEA Y  +       S+             E + + +  N  R+LP
Sbjct: 8   DDAFTEEELLAIDAIEASYNFSRSSSSSSSAAPTVQATTSVHGHEEDPNQIPNNIRRQLP 67

Query: 94  HSILSLQLPYSSPLSPRRVNSKMRFPALNFDGRIIYSRTASEVDRASRELAKKISSTRKG 153
            SI S       PLS  R  +   FPA+ F GRI+YS+TA+EVD+ + +L K + + R  
Sbjct: 68  RSITSSTSYKRFPLSRCRARN---FPAMRFGGRILYSKTATEVDKRAMQLIKVLDTKRDE 127

Query: 154 TDQITIGFDIEWKPTFRRGVPPGKTAVMQLCLDNSQCHVMHIIHSGITPSLKTLLEDDTL 213
           +    +G DIEW+P+FR+GV PGK A +Q+C+D++ C VMHI HSGI  SL+ L+ED TL
Sbjct: 128 SGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHSGIPQSLQHLIEDSTL 187

Query: 214 NKAGVGIANDAVKVFKDYNVSIKAVSEISDLANQKLAGVPQKWGLESLTKALVSKELQKP 273
            K G+GI  D+VK+F DY VSIK V ++SDLANQK+ G  +KWGL SLT+ LV KEL KP
Sbjct: 188 VKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGG-DKKWGLASLTETLVCKELLKP 247

Query: 274 NKIRLGNWEVAVLSKQQLQYAATDAFASWYLHEILKGFPHA 305
           N+IRLGNWE   LSKQQLQYAATDA+ASW+L+++LK  P A
Sbjct: 248 NRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVLKDLPDA 284

[Close]

BLAST of MS005647 vs. ExPASy Swiss-Prot
Match: Q14191 (Werner syndrome ATP-dependent helicase OS=Homo sapiens OX=9606 GN=WRN PE=1 SV=2)

HSP 1 Score: 138.3 bits (347), Expect = 1.5e-31
Identity = 74/192 (38.54%), Postives = 113/192 (58.85%), Query Frame = 0


Query: 109 PALNFDGRIIYSRTASEVDRASRELAKKISSTRKGTDQITIGFDIEWKPTFRRGVPPGKT 168
           P L F G I+YS  AS+    S +++  +S      D   +GFD+EW P + RG   GK 
Sbjct: 45  PFLEFTGSIVYSYDASDCSFLSEDISMSLS------DGDVVGFDMEWPPLYNRG-KLGKV 104

Query: 169 AVMQLCLDNSQCHVMHIIHSGITP-SLKTLLEDDTLNKAGVGIANDAVKVFKDYNVSIKA 228
           A++QLC+  S+C++ H+    + P  LK LLE+  + KAGVGI  D  K+ +D+++ +K 
Sbjct: 105 ALIQLCVSESKCYLFHVSSMSVFPQGLKMLLENKAVKKAGVGIEGDQWKLLRDFDIKLKN 164

Query: 229 VSEISDLANQKLAGVPQKWGLESLTKALVSKELQKPNKIRLGNWEVAVLSKQQLQYAATD 288
             E++D+AN+KL    + W L SL K L+ K+L K   IR  NW    L++ Q  YAATD
Sbjct: 165 FVELTDVANKKLK-CTETWSLNSLVKHLLGKQLLKDKSIRCSNWSKFPLTEDQKLYAATD 224

Query: 289 AFASWYLHEILK 300
           A+A + ++  L+
Sbjct: 225 AYAGFIIYRNLE 228

[Close]

BLAST of MS005647 vs. ExPASy Swiss-Prot
Match: O93530 (Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis OX=8355 GN=wrn PE=2 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 2.2e-30
Identity = 68/193 (35.23%), Postives = 112/193 (58.03%), Query Frame = 0


Query: 109 PALNFDGRIIYSRTASEVDRASRELAKKISSTRKGTDQITIGFDIEWKPTFRRGVPPGKT 168
           P + F+G I+YS  +++    S ++   +       ++  +GFDIEW P + +G   GK 
Sbjct: 39  PFMKFNGSIVYSYESNDCSLLSEDIRSSL------LEEDVLGFDIEWPPVYTKG-KTGKV 98

Query: 169 AVMQLCLDNSQCHVMHII-HSGITPSLKTLLEDDTLNKAGVGIANDAVKVFKDYNVSIKA 228
           A++Q+C+   +C++ HI   +G    LK LLED+++ K GVGI  D  K+  DY + +K 
Sbjct: 99  ALIQVCVSEKKCYLFHISPMAGFPKGLKRLLEDESVRKVGVGIEGDQWKLMSDYELKLKG 158

Query: 229 VSEISDLANQKLAGVPQKWGLESLTKALVSKELQKPNKIRLGNWEVAVLSKQQLQYAATD 288
             E+S++ANQKL    +KW    L K L  ++L K    R  NW++ +L++ Q  YAATD
Sbjct: 159 FIELSEMANQKLR-CKEKWTFNGLIKHLFKEQLYKRKSYRCSNWDIFLLTEDQKLYAATD 218

Query: 289 AFASWYLHEILKG 301
           A+A   +++ L+G
Sbjct: 219 AYAGLLIYKKLEG 223

[Close]

BLAST of MS005647 vs. ExPASy Swiss-Prot
Match: O09053 (Werner syndrome ATP-dependent helicase homolog OS=Mus musculus OX=10090 GN=Wrn PE=1 SV=3)

HSP 1 Score: 132.9 bits (333), Expect = 6.4e-30
Identity = 71/191 (37.17%), Postives = 113/191 (59.16%), Query Frame = 0


Query: 109 PALNFDGRIIYSRTASEVDRASRELAKKISSTRKGTDQITIGFDIEWKPTFRRGVPPGKT 168
           P L F G I+YS  AS+    S +++ ++S      D   +GFD+EW P ++ G    + 
Sbjct: 39  PFLEFPGSIVYSYEASDCSFLSEDISMRLS------DGDVVGFDMEWPPIYKPG-KRSRV 98

Query: 169 AVMQLCLDNSQCHVMHIIHSGITP-SLKTLLEDDTLNKAGVGIANDAVKVFKDYNVSIKA 228
           AV+QLC+  S+C++ HI    + P  LK LLE+ ++ KAGVGI  D  K+ +D++V +++
Sbjct: 99  AVIQLCVSESKCYLFHISSMSVFPQGLKMLLENKSIKKAGVGIEGDQWKLLRDFDVKLES 158

Query: 229 VSEISDLANQKLAGVPQKWGLESLTKALVSKELQKPNKIRLGNWEVAVLSKQQLQYAATD 288
             E++D+AN+KL    + W L  L K ++ K+L K   IR  NW    L++ Q  YAATD
Sbjct: 159 FVELTDVANEKLK-CAETWSLNGLVKHVLGKQLLKDKSIRCSNWSNFPLTEDQKLYAATD 218

Query: 289 AFASWYLHEIL 299
           A+A   +++ L
Sbjct: 219 AYAGLIIYQKL 221

[Close]

BLAST of MS005647 vs. ExPASy Swiss-Prot
Match: B3NZ68 (Werner Syndrome-like exonuclease OS=Drosophila erecta OX=7220 GN=WRNexo PE=3 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.4e-16
Identity = 54/210 (25.71%), Postives = 106/210 (50.48%), Query Frame = 0


Query: 107 RFPALNFDGRIIYSRTASEVDRASRELAKKISSTRKGTDQITIGFDIEWKPTFRRGVPPG 166
           + P + + G I Y   + ++  ++ ++ + +   ++  + + + FD+EW  +F+ G  PG
Sbjct: 120 KLPFIKYKGAIKYYTESQDIAASADDVLQWVE--KQKDEVVPMAFDMEWPFSFQTG--PG 179

Query: 167 KTAVMQLCLDNSQCHVMHIIHSGITPS-LKTLLEDDTLNKAGVGIANDAVKVFKDY-NVS 226
           K+AV+Q+C+D   C++  + +    P+ L  L+    +   GV I ND  K+ +D+  VS
Sbjct: 180 KSAVIQICVDEKCCYIYQLTNLKKLPAVLVALINHSKVRLHGVNIKNDFRKLARDFPEVS 239

Query: 227 IK-AVSEISDLA--NQKLAGVPQKWGLESLTKALVSKELQKPNKIRLGNWEVAVLSKQQL 286
            +  + +  DL     ++     +W LE LT  +  K + K  K+R+  W V  L + QL
Sbjct: 240 AEPLIEKCVDLGVWCNEVCETGGRWSLERLTNFIAKKAMDKSKKVRMSKWHVIPLDENQL 299

Query: 287 QYAATDAFASWYLHEILKGFPHAQKVEDSE 312
            YAA D +    ++  L+    A+ + + E
Sbjct: 300 MYAAIDVYIGQVIYRELERREKAKIINEEE 325

[Close]

BLAST of MS005647 vs. ExPASy TrEMBL
Match: A0A6J1D021 (Werner Syndrome-like exonuclease OS=Momordica charantia OX=3673 GN=LOC111016124 PE=4 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 8.6e-171
Identity = 311/314 (99.04%), Postives = 312/314 (99.36%), Query Frame = 0


Query: 1   MEFPGCSRNAGASPPLSVSSSSFSSRSFSVSEFDQPFTEQQLQEIDAIEAMYQSTSGKRR 60
           MEFPGCSRNAGASPPLSVSSSSFSSRSFSVSEFDQPFTEQQLQEIDAIEAMYQSTSGKRR
Sbjct: 1   MEFPGCSRNAGASPPLSVSSSSFSSRSFSVSEFDQPFTEQQLQEIDAIEAMYQSTSGKRR 60

Query: 61  RGSSTADENEASCVSPNAGRRLPHSILSLQLPYSSPLSPRRVNSKMRFPALNFDGRIIYS 120
           RGSSTADENEASCVSPNAGRRLPHSILSLQLPYSSPLSPRRVNSKMRFPALNFDGRIIYS
Sbjct: 61  RGSSTADENEASCVSPNAGRRLPHSILSLQLPYSSPLSPRRVNSKMRFPALNFDGRIIYS 120

Query: 121 RTASEVDRASRELAKKISSTRKGTDQITIGFDIEWKPTFRRGVPPGKTAVMQLCLDNSQC 180
            TASEVDRASRELAKKISSTRKGTDQITIGFDIEWKPTFRRGVPPGKTAVMQLCLDNSQC
Sbjct: 121 TTASEVDRASRELAKKISSTRKGTDQITIGFDIEWKPTFRRGVPPGKTAVMQLCLDNSQC 180

Query: 181 HVMHIIHSGITPSLKTLLEDDTLNKAGVGIANDAVKVFKDYNVSIKAVSEISDLANQKLA 240
           HVMHIIHSGITPSLKTLLEDDTL+KAGVGIANDAVKVFKDYNVSIKAVSEISDLANQKLA
Sbjct: 181 HVMHIIHSGITPSLKTLLEDDTLSKAGVGIANDAVKVFKDYNVSIKAVSEISDLANQKLA 240

Query: 241 GVPQKWGLESLTKALVSKELQKPNKIRLGNWEVAVLSKQQLQYAATDAFASWYLHEILKG 300
           GVPQKWGL SLTKALVSKELQKPNKIRLGNWEVAVLSKQQLQYAATDAFASWYLHEILKG
Sbjct: 241 GVPQKWGLGSLTKALVSKELQKPNKIRLGNWEVAVLSKQQLQYAATDAFASWYLHEILKG 300

Query: 301 FPHAQKVEDSERDM 315
           FPHAQKVEDSERDM
Sbjct: 301 FPHAQKVEDSERDM 314

[Close]

BLAST of MS005647 vs. ExPASy TrEMBL
Match: A0A1S3AWQ6 (Werner Syndrome-like exonuclease OS=Cucumis melo OX=3656 GN=LOC103483463 PE=4 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 3.0e-131
Identity = 242/311 (77.81%), Postives = 270/311 (86.82%), Query Frame = 0


Query: 1   MEFPGCSRNAGASPPLSVSSSSFSSRSFSVSEFDQPFTEQQLQEIDAIEAMYQSTSGKRR 60
           MEFPG S +  +S  LS  SSSF S SFS SEFDQPFTEQQLQ I+AIEA YQSTS K+R
Sbjct: 1   MEFPGSSNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60

Query: 61  RGSSTADENEASCVSPNAGRRLPHSILSLQLPYSSPLSPRRVNSKMRFPALNFDGRIIYS 120
           R +ST DEN  SCVSPN GRRLP SI S Q P+ SPLS  RVNSKMRFPAL++ GRIIYS
Sbjct: 61  RPNSTLDENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYS 120

Query: 121 RTASEVDRASRELAKKISSTRKGTDQITIGFDIEWKPTFRRGVPPGKTAVMQLCLDNSQC 180
           RT SEVDRASRELAKKI+STRKG +QITIGFDIEW+P+F+RGVPPGK AVMQLCL+NS+C
Sbjct: 121 RTVSEVDRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180

Query: 181 HVMHIIHSGITPSLKTLLEDDTLNKAGVGIANDAVKVFKDYNVSIKAVSEISDLANQKLA 240
           HVMHIIHSGI  SL+ LLEDDTLNKAGVGIA+DA KVFKDYNVS+K + EISDLANQKLA
Sbjct: 181 HVMHIIHSGIPQSLQALLEDDTLNKAGVGIASDASKVFKDYNVSMKQLDEISDLANQKLA 240

Query: 241 GVPQKWGLESLTKALVSKELQKPNKIRLGNWEVAVLSKQQLQYAATDAFASWYLHEILKG 300
           GVP+KWGL +LT+ L+SKEL+KP++IRLGNWEVAVLSK+QL YAATDAFASWYLHEILKG
Sbjct: 241 GVPKKWGLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKG 300

Query: 301 FPHAQKVEDSE 312
           FPH +KV DSE
Sbjct: 301 FPHVEKVADSE 311

[Close]

BLAST of MS005647 vs. ExPASy TrEMBL
Match: A0A6J1G5L3 (Werner Syndrome-like exonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451092 PE=4 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 3.9e-131
Identity = 250/313 (79.87%), Postives = 267/313 (85.30%), Query Frame = 0


Query: 1   MEFPGCSRNAGASPPLSVSSSSFSSRSFSVSEFDQPFTEQQLQEIDAIEAMYQSTSGKRR 60
           MEFP  S  AGAS  LSV SS  SS SFS+SEFDQPFTEQQLQ I+AIEA YQ+T+ KRR
Sbjct: 1   MEFPCSSNRAGASTELSVCSSPSSSYSFSLSEFDQPFTEQQLQAIEAIEAAYQATTAKRR 60

Query: 61  RGSSTADENEASCVSPNAGRRLPHSILSLQLPYSSPLSPRRVNSKMRFPALNFDGRIIYS 120
           R      ENE  CVSPN+GRRLP SI SLQ P+SSPLSP RV SKMRFPAL F GRI+YS
Sbjct: 61  R------ENEVLCVSPNSGRRLPRSISSLQSPHSSPLSPCRVTSKMRFPALKFGGRIVYS 120

Query: 121 RTASEVDRASRELAKKISSTRKGTDQITIGFDIEWKPTFRRGVPPGKTAVMQLCLDNSQC 180
           RTASEVDRASRELAKKI+S RKG DQITIGFDIEWKPTFRRGVPPGK AVMQLCLD+SQC
Sbjct: 121 RTASEVDRASRELAKKINSMRKGMDQITIGFDIEWKPTFRRGVPPGKAAVMQLCLDHSQC 180

Query: 181 HVMHIIHSGITPSLKTLLEDDTLNKAGVGIANDAVKVFKDYNVSIKAVSEISDLANQKLA 240
           HVMHIIHSGI  SL+ LLEDDTLNKAGVGI NDA+KVFKDYNVSIKAV EIS+LANQKLA
Sbjct: 181 HVMHIIHSGIPRSLQALLEDDTLNKAGVGIVNDAMKVFKDYNVSIKAVDEISNLANQKLA 240

Query: 241 GVPQKWGLESLTKALVSKELQKPNKIRLGNWEVAVLSKQQLQYAATDAFASWYLHEILKG 300
           GVP+KW L SLT+AL+SKEL KP+KIRLGNWEVAVLSK+QL YAATDAFASWYLHEILKG
Sbjct: 241 GVPKKWSLGSLTEALISKELPKPSKIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKG 300

Query: 301 FPHAQKVEDSERD 314
           FP  Q   DSE D
Sbjct: 301 FPDPQAAADSEGD 307

[Close]

BLAST of MS005647 vs. ExPASy TrEMBL
Match: A0A0A0L9B9 (3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G129680 PE=4 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 3.3e-130
Identity = 241/311 (77.49%), Postives = 269/311 (86.50%), Query Frame = 0


Query: 1   MEFPGCSRNAGASPPLSVSSSSFSSRSFSVSEFDQPFTEQQLQEIDAIEAMYQSTSGKRR 60
           MEF G S ++ +S  LS  SSSF   SFS SEFDQPFTEQQLQ I+AIEA YQSTS K+R
Sbjct: 1   MEFRGSSNSSHSSTELSACSSSFPPWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60

Query: 61  RGSSTADENEASCVSPNAGRRLPHSILSLQLPYSSPLSPRRVNSKMRFPALNFDGRIIYS 120
           R +ST D NE SCVSP+ GRRLP SI S Q P  SPLSP RVNSKMRFPALN+ GRIIYS
Sbjct: 61  RPNSTPDGNEVSCVSPDTGRRLPRSIFSHQSPRFSPLSPCRVNSKMRFPALNYGGRIIYS 120

Query: 121 RTASEVDRASRELAKKISSTRKGTDQITIGFDIEWKPTFRRGVPPGKTAVMQLCLDNSQC 180
           RT SEVDRASRELAKKI+STRK  DQITIGFDIEW+P+F+RGVPPGK AVMQLCL+NS+C
Sbjct: 121 RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180

Query: 181 HVMHIIHSGITPSLKTLLEDDTLNKAGVGIANDAVKVFKDYNVSIKAVSEISDLANQKLA 240
           HVMHIIHSGI  SL+ LLEDDTL+KAGVGIA+DA KVFK+YNVS+K ++EISDLANQKLA
Sbjct: 181 HVMHIIHSGIPQSLQALLEDDTLSKAGVGIASDASKVFKEYNVSVKPLNEISDLANQKLA 240

Query: 241 GVPQKWGLESLTKALVSKELQKPNKIRLGNWEVAVLSKQQLQYAATDAFASWYLHEILKG 300
           GVP+KWGL +LT+ L+SKELQKP++IRLGNWEVAVLSK QLQYAATDAFASWYLHEILKG
Sbjct: 241 GVPKKWGLRALTETLISKELQKPDRIRLGNWEVAVLSKDQLQYAATDAFASWYLHEILKG 300

Query: 301 FPHAQKVEDSE 312
           FPH +KV DSE
Sbjct: 301 FPHVEKVADSE 311

[Close]

BLAST of MS005647 vs. ExPASy TrEMBL
Match: A0A5A7TZU7 (Werner Syndrome-like exonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G002920 PE=4 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 4.8e-129
Identity = 238/306 (77.78%), Postives = 266/306 (86.93%), Query Frame = 0


Query: 6   CSRNAGASPPLSVSSSSFSSRSFSVSEFDQPFTEQQLQEIDAIEAMYQSTSGKRRRGSST 65
           CS +  +S  LS  SSSF S SFS SEFDQPFTEQQLQ I+AIEA YQSTS K+RR +ST
Sbjct: 31  CSNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKRRPNST 90

Query: 66  ADENEASCVSPNAGRRLPHSILSLQLPYSSPLSPRRVNSKMRFPALNFDGRIIYSRTASE 125
            DEN  SCVSPN GRRLP SI S Q P+ SPLS  RVNSKMRFPAL++ GRIIYSRT SE
Sbjct: 91  LDENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYSRTVSE 150

Query: 126 VDRASRELAKKISSTRKGTDQITIGFDIEWKPTFRRGVPPGKTAVMQLCLDNSQCHVMHI 185
           VDRASRELAKKI+STRKG +QITIGFDIEW+P+F+RGVPPGK AVMQLCL+NS+CHVMHI
Sbjct: 151 VDRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHI 210

Query: 186 IHSGITPSLKTLLEDDTLNKAGVGIANDAVKVFKDYNVSIKAVSEISDLANQKLAGVPQK 245
           IHSGI  SL+ LLEDDTLNKAGVGIA+DA KVFKDYNVS+K + EISDLANQKLAGVP+K
Sbjct: 211 IHSGIPQSLQALLEDDTLNKAGVGIASDASKVFKDYNVSMKQLDEISDLANQKLAGVPKK 270

Query: 246 WGLESLTKALVSKELQKPNKIRLGNWEVAVLSKQQLQYAATDAFASWYLHEILKGFPHAQ 305
           WGL +LT+ L+SKEL+KP++IRLGNWEVAVLSK+QL YAATDAFASWYLHEILKGFPH +
Sbjct: 271 WGLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKGFPHVE 330

Query: 306 KVEDSE 312
           KV DSE
Sbjct: 331 KVADSE 336

[Close]

BLAST of MS005647 vs. TAIR 10
Match: AT4G13870.2 (Werner syndrome-like exonuclease )

HSP 1 Score: 262.7 bits (670), Expect = 3.8e-70
Identity = 141/281 (50.18%), Postives = 185/281 (65.84%), Query Frame = 0


Query: 34  DQPFTEQQLQEIDAIEAMYQSTSGKRRRGSST----------ADENEASCVSPNAGRRLP 93
           D  FTE++L  IDAIEA Y  +       S+             E + + +  N  R+LP
Sbjct: 8   DDAFTEEELLAIDAIEASYNFSRSSSSSSSAAPTVQATTSVHGHEEDPNQIPNNIRRQLP 67

Query: 94  HSILSLQLPYSSPLSPRRVNSKMRFPALNFDGRIIYSRTASEVDRASRELAKKISSTRKG 153
            SI S       PLS  R  +   FPA+ F GRI+YS+TA+EVD+ + +L K + + R  
Sbjct: 68  RSITSSTSYKRFPLSRCRARN---FPAMRFGGRILYSKTATEVDKRAMQLIKVLDTKRDE 127

Query: 154 TDQITIGFDIEWKPTFRRGVPPGKTAVMQLCLDNSQCHVMHIIHSGITPSLKTLLEDDTL 213
           +    +G DIEW+P+FR+GV PGK A +Q+C+D++ C VMHI HSGI  SL+ L+ED TL
Sbjct: 128 SGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHSGIPQSLQHLIEDSTL 187

Query: 214 NKAGVGIANDAVKVFKDYNVSIKAVSEISDLANQKLAGVPQKWGLESLTKALVSKELQKP 273
            K G+GI  D+VK+F DY VSIK V ++SDLANQK+ G  +KWGL SLT+ LV KEL KP
Sbjct: 188 VKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGG-DKKWGLASLTETLVCKELLKP 247

Query: 274 NKIRLGNWEVAVLSKQQLQYAATDAFASWYLHEILKGFPHA 305
           N+IRLGNWE   LSKQQLQYAATDA+ASW+L+++LK  P A
Sbjct: 248 NRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVLKDLPDA 284

[Close]

BLAST of MS005647 vs. TAIR 10
Match: AT4G13870.1 (Werner syndrome-like exonuclease )

HSP 1 Score: 254.6 bits (649), Expect = 1.0e-67
Identity = 138/275 (50.18%), Postives = 181/275 (65.82%), Query Frame = 0


Query: 34  DQPFTEQQLQEIDAIEAMYQSTSGKRRRGSST----------ADENEASCVSPNAGRRLP 93
           D  FTE++L  IDAIEA Y  +       S+             E + + +  N  R+LP
Sbjct: 8   DDAFTEEELLAIDAIEASYNFSRSSSSSSSAAPTVQATTSVHGHEEDPNQIPNNIRRQLP 67

Query: 94  HSILSLQLPYSSPLSPRRVNSKMRFPALNFDGRIIYSRTASEVDRASRELAKKISSTRKG 153
            SI S       PLS  R  +   FPA+ F GRI+YS+TA+EVD+ + +L K + + R  
Sbjct: 68  RSITSSTSYKRFPLSRCRARN---FPAMRFGGRILYSKTATEVDKRAMQLIKVLDTKRDE 127

Query: 154 TDQITIGFDIEWKPTFRRGVPPGKTAVMQLCLDNSQCHVMHIIHSGITPSLKTLLEDDTL 213
           +    +G DIEW+P+FR+GV PGK A +Q+C+D++ C VMHI HSGI  SL+ L+ED TL
Sbjct: 128 SGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHSGIPQSLQHLIEDSTL 187

Query: 214 NKAGVGIANDAVKVFKDYNVSIKAVSEISDLANQKLAGVPQKWGLESLTKALVSKELQKP 273
            K G+GI  D+VK+F DY VSIK V ++SDLANQK+ G  +KWGL SLT+ LV KEL KP
Sbjct: 188 VKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGG-DKKWGLASLTETLVCKELLKP 247

Query: 274 NKIRLGNWEVAVLSKQQLQYAATDAFASWYLHEIL 299
           N+IRLGNWE   LSKQQLQYAATDA+ASW+L++ L
Sbjct: 248 NRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKDL 278

[Close]

BLAST of MS005647 vs. TAIR 10
Match: AT4G13885.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 89.0 bits (219), Expect = 7.5e-18
Identity = 50/111 (45.05%), Postives = 60/111 (54.05%), Query Frame = 0


Query: 194 LKTLLEDDTLNKAGVGIANDAVKVFKDYNVSIKAVSEISDLANQKLAGVPQKWGLESLTK 253
           L+ L+ED TL K G+GI  D+VK+F DY                                
Sbjct: 186 LQHLIEDSTLVKVGIGIDGDSVKLFHDYG------------------------------- 245

Query: 254 ALVSKELQKPNKIRLGNWEVAVLSKQQLQYAATDAFASWYLHEILKGFPHA 305
                 L KPN+IRLGNWEV  LSKQQLQYAATDA+ASW+LH++LK  P A
Sbjct: 246 ------LLKPNRIRLGNWEVHPLSKQQLQYAATDAYASWHLHQVLKDLPDA 259

[Close]

BLAST of MS005647 vs. TAIR 10
Match: AT1G56310.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 52.8 bits (125), Expect = 6.0e-07
Identity = 47/188 (25.00%), Postives = 81/188 (43.09%), Query Frame = 0


Query: 136 KISSTRKGTDQI----TIGFDIEWKPTFRRGVPPGKTAVMQLCLD------------NSQ 195
           +++  RK T  +     +G D EWKP + +G    K ++MQ+  D            N  
Sbjct: 364 EVNELRKATSFLEGCRVVGIDCEWKPNYIKGSKQNKVSIMQIGSDTKIFILDLIKLYNDA 423

Query: 196 CHVM-----HIIHSGITPSLKTLLEDDTLNKAGVGIANDAVKVFKDYNVSIKAVSEISDL 255
             ++     HI+ S  T  L    + D    A   ++   +K F+ Y++ +    +I ++
Sbjct: 424 SEILDNCLSHILQSKSTLKLGYNFQCDIKQLA---LSYGDLKCFERYDMLL----DIQNV 483

Query: 256 ANQKLAGVPQKWGLESLTKALVSKELQKPNKIRLGNWEVAVLSKQQLQYAATDAFASWYL 303
            N+         GL  LTK ++   L K    R  +WE   LS+ QL+YAA DA    ++
Sbjct: 484 FNEPFG------GLAGLTKKILGVSLNKTR--RNSDWEQRPLSQNQLEYAALDAAVLIHI 536

[Close]

BLAST of MS005647 vs. TAIR 10
Match: AT5G48350.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 51.6 bits (122), Expect = 1.3e-06
Identity = 49/194 (25.26%), Postives = 84/194 (43.30%), Query Frame = 0


Query: 122 TASEVDRASRELAKKISSTRKGTDQITIGFDIEWKPTFRRGVPPGKTAVMQLCLDNSQCH 181
           T +E +R    L K   S +    +I IG D E     ++G    KT ++QLC D   C 
Sbjct: 16  TVTEKERDINRLVKTFLSNKNNRKKI-IGLDTE---RVQKGRKLNKTVLLQLC-DGDNCL 75

Query: 182 VMHI----------IHSGITPSLKTLLEDDTLNKAGVGIANDAVKVFKDYNVSIKAVSEI 241
           ++ +              +   L   L        G+GI    +++  ++ ++ K V EI
Sbjct: 76  IVQLPDEDEDEGEGEDDNLPLPLFNFLNLPEFTFVGIGINKTMMRLESEFGLTCKNVVEI 135

Query: 242 SDLANQKLAGVPQKWGLESLT-------KALVSKELQKPNKIRLGNWEVAVLSKQQLQYA 299
                      P  W L ++T        A+VS E  +P+   L +WE  VL+K Q++ A
Sbjct: 136 G----------PATWNLTNMTTDVKFRISAIVSTE--RPSNAVLEDWEKFVLNKNQIKLA 192

The following BLAST results are available for this feature:

BLAST of MS005647 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147109.1	1.8e-170	99.04	Werner Syndrome-like exonuclease [Momordica charantia]	[more]
XP_038897657.1	1.0e-133	82.69	Werner Syndrome-like exonuclease [Benincasa hispida]	[more]
KAG7028742.1	7.3e-132	80.19	Werner Syndrome-like exonuclease [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6597271.1	9.5e-132	79.87	Werner Syndrome-like exonuclease, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_008438323.1	6.2e-131	77.81	PREDICTED: Werner Syndrome-like exonuclease [Cucumis melo]	[more]

BLAST of MS005647 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84LH3	5.3e-69	50.18	Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=...	[more]
Q14191	1.5e-31	38.54	Werner syndrome ATP-dependent helicase OS=Homo sapiens OX=9606 GN=WRN PE=1 SV=2	[more]
O93530	2.2e-30	35.23	Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis OX=8355 GN=wrn ...	[more]
O09053	6.4e-30	37.17	Werner syndrome ATP-dependent helicase homolog OS=Mus musculus OX=10090 GN=Wrn P...	[more]
B3NZ68	1.4e-16	25.71	Werner Syndrome-like exonuclease OS=Drosophila erecta OX=7220 GN=WRNexo PE=3 SV=...	[more]

BLAST of MS005647 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D021	8.6e-171	99.04	Werner Syndrome-like exonuclease OS=Momordica charantia OX=3673 GN=LOC111016124 ...	[more]
A0A1S3AWQ6	3.0e-131	77.81	Werner Syndrome-like exonuclease OS=Cucumis melo OX=3656 GN=LOC103483463 PE=4 SV...	[more]
A0A6J1G5L3	3.9e-131	79.87	Werner Syndrome-like exonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451092 P...	[more]
A0A0A0L9B9	3.3e-130	77.49	3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G...	[more]
A0A5A7TZU7	4.8e-129	77.78	Werner Syndrome-like exonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]

BLAST of MS005647 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G13870.2	3.8e-70	50.18	Werner syndrome-like exonuclease	[more]
AT4G13870.1	1.0e-67	50.18	Werner syndrome-like exonuclease	[more]
AT4G13885.1	7.5e-18	45.05	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G56310.1	6.0e-07	25.00	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT5G48350.1	1.3e-06	25.26	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002562	3'-5' exonuclease domain	SMART	SM00474	35exoneu6	coord: 117..303 e-value: 2.5E-18 score: 76.9
IPR002562	3'-5' exonuclease domain	PFAM	PF01612	DNA_pol_A_exo1	coord: 132..299 e-value: 3.5E-17 score: 62.7
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 100..301 e-value: 8.1E-53 score: 180.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..23
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 54..77
None	No IPR available	PANTHER	PTHR13620:SF45	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 70..306
None	No IPR available	PANTHER	PTHR13620	3-5 EXONUCLEASE	coord: 70..306
None	No IPR available	CDD	cd06141	WRN_exo	coord: 121..299 e-value: 2.62724E-55 score: 175.459
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 148..299

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS005647.1	MS005647.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006139	nucleobase-containing compound metabolic process
molecular_function	GO:0008408	3'-5' exonuclease activity
molecular_function	GO:0003676	nucleic acid binding