MS005615 (gene) Bitter gourd (TR) v1

Overview


Name	MS005615
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	cytochrome b5-like
Location	scaffold254: 297581 .. 300232 (-)
RNA-Seq Expression	MS005615
Synteny	MS005615

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTAGCGGGGAAAAGGTTTTCACCTTGAAGGAAGTCGCCGAGCACAACAACCACAGGGATTGTTGGCTCATCATCAGTGGCAAGGTATAAAAATCCATCAATCTCCCTTTTTCTTCTTCTTCTGGATCTACGATTTTTCATTTATCTCGCCGATTCTGCTATTTTCGCCGCCGATCTCTTGCCACGGGGTGAATTCTGATCCTTGTTGTGTGGATGGAGTGGAGAGTTTTCGCTGCTTCTTTAGATTTCGTTTCAGATTCGTGTGTTTGGCTCGTTTGAGATTTCAGATTTATGGTCTTCTGATTGTAGTGGTTGAAACTTGAAATGATAATGTTAGCTGTTTAGATCTGTGACTTTTAGATAGGACTTTATGGCCTTTTTGCTGCCTCTTCTTGAAGAATATCACATGCAGAGGGGGAGGGATTACAATTTTAACTGTGCTTAGGTCTATTTTCTGTCTGTGTTTGCTTCCTCATTTAGGGTTACGCTATCATAATATGCACATTTTATTCTTCTCTGTGTTTCCTGATTTATTTCAAGAGCCCAACGTTTTTGTCTACTAGTGCTTTATTTATTTATTTTGATAAGAATTGAAACTATGTCATTGAAAAAGACGCATGAAAAAGAACAACATAAAGGCTTCAGGGTCTCTGCCCTAAGCTTAAAAGGAGGGGTACCATTAGAAGGTATGTTGCAAAACTATTTGTGAAGAGGACTCTAATTAGAGGCAGTTTTGTTGTACATGATTTATGAAATTAACAAAAGAATCTAACTTATGAGAGTAGATCCTAGAATTTCTCTCTTTCAAAAGTAGCCAAAGAAAAGATAGTGTTGGACTTATTGTTTTATAATGTTGGTCGTCCCAATCTACTCTTATTTTTTGCTTGCTTGCGATGTTTTCCTGATTACTATGCTGTTCTGGACATTGTTTGATTGCCAATGTGCTGTTGGAATGGTGAGCTGGTGGATAATCTTCACCTGTCTTCCACTTCTGTAGAAAACTTACTGAACGTTACATCTTAATATCCATATGTACAATTTATTGTTTAAAGATTCCTTGAACGGGGAGAGAATTTTCTTTGCCAGAAGGGCTACGGATTCGTAGTTCTTCACAAACCAACAAACCTTTGGCTGATGTAGTTTAGAGTTTACAGCTTAGCTTGTTTAACGACTCGGGTATCTCATGATAGTCATGGTCGAAATTATTATAATATAAGTTTGTTTGATTGATGAAAATTCATGACCAAAACCAATGCTTCTTAGTTTCAAGTTAAAGATAGTGATGGCATGAAGATTTATAAGTAATCAGTTCCTAGTCTTCAATAATGTTTTCCTTTACTCATGTACATGTTGAGCACATTGCTGACCGATGTGGAACGAATGTGTAGCTGTTCCATTGTCACGTTCTTTATGACCTGAAAAACCTTCTATCATAATCTTTCTGGACTTCTTTGCTTTATGTTTTAAGCCCATGATTATATTACTAAAGATTCATGTAAGCTACATTGTTGTAGAAAGAAATGTCTTATGCCAATGATCAAGAAATGGGCATTCAGATTAAGGAGTAAAATTTACCTGTGTATGGACAGATTCTTTACTTCAAGGTGCTTTGGGTGTGTATTTCCAACTGATTTTGAGATTTTTCCTGATACTCTTCGGCATTATGCATAATCAAAAAGATGGTAGTTGGAATCTTAAATCTCGTATTCTCATCTCCAAGGGGCTTTAGGATTGGGATGCTTGGACTTGAAGATACCCTCTCCAAAGAGTTCAAGTTTTCAACTTGAAAACTTAGGTGTCGTTAGATAATTATTATTTTTTTTTAGTTTTCTATTTTTAAAAATTATGCTTGTTTTTCACCAATCTTGATAACGTATTTAAATTCTGAGCCAAATTTCAAAAACAGTAACAAAAACTTTTGAAAATGATTTGAAAAATTATTAATTATATAACAAAACAAAGGGATACGGGTGAAAGTTTCTTAGCTTAATTTTTAAAGACAAAACCAAAAACGAAATGGTTATTTTACGGGGCCTTAATATTTTAAAATTGTTGTCTTTTGCGACCTTCTAGAGATTGGTGGGGTGGGCCCAAAAATAAACTCCCATTCTCATGATTTATAAAAGCTGCATTTACTATAGAAGAGTGTTGAATCTGACCAGGAGATCTTCTGCTTTATTTTATATCAGTTATCTAATCTCCTTGGTCCCTTCGATGATCCGAGCAGGTATATGATGTGACCAAATTCTTAGAAGACCATCCTGGTGGTGATGACGTTTTGTTATCAGCTACAGGTAAATACCAAGTACTTCAGTACGTATATTTTCAAACCTAACTGCTCTCTGTAGTTTGTGTTCCCTTATTGAAATAATGCCTAACATTTACATGGTGCTACTCTCAGGAAAAGATGCGACCGACGATTTTGAAGACGTGGGGCATAGTGACAATGCAAGAGAAATGATGGATCAATACTATGTCGGGGAGATTGATGGTTCAACAATTCCCAAGAAGGTCACATACACACCTCCAAAACAGCCTCACTACAATCAGGACAAGACATCTGAGTTCATCATCAAGCTCCTCCAGTTCCTCGTTCCGCTTGCCATTTTGGGATTGGCAGTTGCCATTCGGTTCTACACCAAACAAGCT

mRNA sequence

ATGGGTAGCGGGGAAAAGGTTTTCACCTTGAAGGAAGTCGCCGAGCACAACAACCACAGGGATTGTTGGCTCATCATCAGTGGCAAGGTATATGATGTGACCAAATTCTTAGAAGACCATCCTGGTGGTGATGACGTTTTGTTATCAGCTACAGGAAAAGATGCGACCGACGATTTTGAAGACGTGGGGCATAGTGACAATGCAAGAGAAATGATGGATCAATACTATGTCGGGGAGATTGATGGTTCAACAATTCCCAAGAAGGTCACATACACACCTCCAAAACAGCCTCACTACAATCAGGACAAGACATCTGAGTTCATCATCAAGCTCCTCCAGTTCCTCGTTCCGCTTGCCATTTTGGGATTGGCAGTTGCCATTCGGTTCTACACCAAACAAGCT

Coding sequence (CDS)

ATGGGTAGCGGGGAAAAGGTTTTCACCTTGAAGGAAGTCGCCGAGCACAACAACCACAGGGATTGTTGGCTCATCATCAGTGGCAAGGTATATGATGTGACCAAATTCTTAGAAGACCATCCTGGTGGTGATGACGTTTTGTTATCAGCTACAGGAAAAGATGCGACCGACGATTTTGAAGACGTGGGGCATAGTGACAATGCAAGAGAAATGATGGATCAATACTATGTCGGGGAGATTGATGGTTCAACAATTCCCAAGAAGGTCACATACACACCTCCAAAACAGCCTCACTACAATCAGGACAAGACATCTGAGTTCATCATCAAGCTCCTCCAGTTCCTCGTTCCGCTTGCCATTTTGGGATTGGCAGTTGCCATTCGGTTCTACACCAAACAAGCT

Protein sequence

MGSGEKVFTLKEVAEHNNHRDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFEDVGHSDNAREMMDQYYVGEIDGSTIPKKVTYTPPKQPHYNQDKTSEFIIKLLQFLVPLAILGLAVAIRFYTKQA

Homology

[Close]

BLAST of MS005615 vs. NCBI nr
Match: XP_022147043.1 (cytochrome b5-like [Momordica charantia])

HSP 1 Score: 278.5 bits (711), Expect = 3.1e-71
Identity = 134/134 (100.00%), Postives = 134/134 (100.00%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHRDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 60
           MGSGEKVFTLKEVAEHNNHRDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE
Sbjct: 1   MGSGEKVFTLKEVAEHNNHRDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVTYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHSDNAREMMDQYYVGEIDGSTIPKKVTYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVTYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVAIRFYTKQA 135
           LGLAVAIRFYTKQA
Sbjct: 121 LGLAVAIRFYTKQA 134

[Close]

BLAST of MS005615 vs. NCBI nr
Match: XP_022936377.1 (cytochrome b5-like [Cucurbita moschata] >XP_022974878.1 cytochrome b5-like [Cucurbita maxima] >XP_023539499.1 cytochrome b5-like [Cucurbita pepo subsp. pepo] >KAG7028761.1 Cytochrome b5 isoform B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 273.5 bits (698), Expect = 9.9e-70
Identity = 130/134 (97.01%), Postives = 133/134 (99.25%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHRDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 60
           MGSGEKVFTLKEVAEHNNH+DCWLIISGKVYDVTKFL+DHPGGDDVLLSATGKDATDDFE
Sbjct: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVTYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHSDNAREMMDQYYVGEIDGSTIPKKV YTPPKQPHYNQDKTSEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVAIRFYTKQA 135
           LGLAVA+RFYTKQA
Sbjct: 121 LGLAVALRFYTKQA 134

[Close]

BLAST of MS005615 vs. NCBI nr
Match: XP_008438367.1 (PREDICTED: cytochrome b5 [Cucumis melo] >XP_038884390.1 cytochrome b5-like [Benincasa hispida] >KAA0049155.1 cytochrome b5 [Cucumis melo var. makuwa] >TYK17406.1 cytochrome b5 [Cucumis melo var. makuwa])

HSP 1 Score: 273.1 bits (697), Expect = 1.3e-69
Identity = 131/134 (97.76%), Postives = 132/134 (98.51%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHRDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 60
           MGSGEKVFTLKEVAEHNNH+DCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE
Sbjct: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVTYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHSDNAREMMDQYYVGEID STIPKKV YTPPKQPHYNQDKTSEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSDNAREMMDQYYVGEIDASTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVAIRFYTKQA 135
           LGLAVAIRFYTKQA
Sbjct: 121 LGLAVAIRFYTKQA 134

[Close]

BLAST of MS005615 vs. NCBI nr
Match: XP_004133998.1 (cytochrome b5 [Cucumis sativus] >KAE8650304.1 hypothetical protein Csa_009551 [Cucumis sativus])

HSP 1 Score: 273.1 bits (697), Expect = 1.3e-69
Identity = 131/134 (97.76%), Postives = 132/134 (98.51%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHRDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 60
           MGSGEKVFTLKEVAEHNNH+DCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE
Sbjct: 68  MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 127

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVTYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHSDNAREMMDQYYVGEID STIPKKV YTPPKQPHYNQDKTSEFIIKLLQFLVPLAI
Sbjct: 128 DVGHSDNAREMMDQYYVGEIDSSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 187

Query: 121 LGLAVAIRFYTKQA 135
           LGLAVAIRFYTKQA
Sbjct: 188 LGLAVAIRFYTKQA 201

[Close]

BLAST of MS005615 vs. NCBI nr
Match: XP_023521594.1 (cytochrome b5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 273.1 bits (697), Expect = 1.3e-69
Identity = 129/134 (96.27%), Postives = 133/134 (99.25%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHRDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 60
           MGSGEKVFTLKEVAEHNNH+DCWLIISGKVYDVTKFL+DHPGGDDVLLSATGKDATDDFE
Sbjct: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVTYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHSDNAREMMDQYYVGEIDGST+PKKV YTPPKQPHYNQDKTSEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSDNAREMMDQYYVGEIDGSTVPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVAIRFYTKQA 135
           LGLAVA+RFYTKQA
Sbjct: 121 LGLAVALRFYTKQA 134

[Close]

BLAST of MS005615 vs. ExPASy Swiss-Prot
Match: O48845 (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 3.9e-61
Identity = 110/132 (83.33%), Postives = 121/132 (91.67%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHRDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 60
           MG   K+FTL EV+EHN   DCW++I+GKVY+VTKFLEDHPGGDDVLLS+TGKDATDDFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVTYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHS++AREMM+QYYVGEID +TIPKKV YTPPKQPHYNQDKTSEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVAIRFYTK 133
           LGLAV IR YTK
Sbjct: 121 LGLAVGIRIYTK 132

[Close]

BLAST of MS005615 vs. ExPASy Swiss-Prot
Match: P49098 (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 3.7e-59
Identity = 104/134 (77.61%), Postives = 119/134 (88.81%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHRDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 60
           MG   KVFTL EV++HNN +DCWL+ISGKVYDVTKFL+DHPGGD+VLLSATGKDATDDFE
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVTYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHS +AR M+D+YYVG+ID +TIP K  YTPP QPHYNQDKTSEF++KLLQFLVPL I
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYNQDKTSEFVVKLLQFLVPLII 120

Query: 121 LGLAVAIRFYTKQA 135
           LG+A  IRFYTKQ+
Sbjct: 121 LGVAFGIRFYTKQS 134

[Close]

BLAST of MS005615 vs. ExPASy Swiss-Prot
Match: P49099 (Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 1.8e-58
Identity = 104/134 (77.61%), Postives = 118/134 (88.06%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHRDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 60
           MG   KVFTL EV+ HNN +DCWLIISGKVY+VTKFLEDHPGG +VLLSATGKDATDDFE
Sbjct: 1   MGGQSKVFTLAEVSNHNNAKDCWLIISGKVYNVTKFLEDHPGGGEVLLSATGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVTYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           D+GHS +AR M+D+YYVG+ID STIP KV YTPPKQPHYNQDKT+EFI+KLLQFLVPL I
Sbjct: 61  DIGHSSSARAMLDEYYVGDIDSSTIPTKVKYTPPKQPHYNQDKTTEFIVKLLQFLVPLII 120

Query: 121 LGLAVAIRFYTKQA 135
           LG+A  + FYTKQ+
Sbjct: 121 LGVAFGVHFYTKQS 134

[Close]

BLAST of MS005615 vs. ExPASy Swiss-Prot
Match: P49100 (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 219.2 bits (557), Expect = 2.9e-56
Identity = 103/128 (80.47%), Postives = 115/128 (89.84%), Query Frame = 0


Query: 5   EKVFTLKEVAEHNNHRDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFEDVGH 64
           +KV+TL+EVA+HN+  DCWLII GKVY+V+KFLEDHPGGDDVLLS+TGKDATDDFEDVGH
Sbjct: 6   KKVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFEDVGH 65

Query: 65  SDNAREMMDQYYVGEIDGSTIPKKVTYTPPKQPHYNQDKTSEFIIKLLQFLVPLAILGLA 124
           S  AR MMD+YYVG+ID STIP +  Y PPKQPHYNQDKT EFIIK+LQFLVPLAILGLA
Sbjct: 66  STTARAMMDEYYVGDIDTSTIPARTKYVPPKQPHYNQDKTPEFIIKILQFLVPLAILGLA 125

Query: 125 VAIRFYTK 133
           VAIR YTK
Sbjct: 126 VAIRIYTK 133

[Close]

BLAST of MS005615 vs. ExPASy Swiss-Prot
Match: O04354 (Cytochrome b5 OS=Borago officinalis OX=13363 PE=2 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 2.4e-55
Identity = 99/129 (76.74%), Postives = 114/129 (88.37%), Query Frame = 0


Query: 6   KVFTLKEVAEHNNHRDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFEDVGHS 65
           K+FTL EVA+HNN +DCWLII+GKVYDVTKFLEDHPGGDDVLLSATGKDATDDFED+GHS
Sbjct: 3   KIFTLAEVAQHNNSKDCWLIINGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFEDIGHS 62

Query: 66  DNAREMMDQYYVGEIDGSTIPKKVTYTPPKQPHYNQDKTSEFIIKLLQFLVPLAILGLAV 125
            +A+ M+D+YYVG+ID S+IP +V YTPPKQP YN DKT EF+IKLLQFLVPL IL  A+
Sbjct: 63  SSAKAMLDEYYVGDIDSSSIPSQVKYTPPKQPLYNPDKTREFVIKLLQFLVPLVILAGAI 122

Query: 126 AIRFYTKQA 135
            IRFYTK +
Sbjct: 123 GIRFYTKSS 131

[Close]

BLAST of MS005615 vs. ExPASy TrEMBL
Match: A0A6J1D069 (cytochrome b5-like OS=Momordica charantia OX=3673 GN=LOC111016073 PE=3 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.5e-71
Identity = 134/134 (100.00%), Postives = 134/134 (100.00%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHRDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 60
           MGSGEKVFTLKEVAEHNNHRDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE
Sbjct: 1   MGSGEKVFTLKEVAEHNNHRDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVTYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHSDNAREMMDQYYVGEIDGSTIPKKVTYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVTYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVAIRFYTKQA 135
           LGLAVAIRFYTKQA
Sbjct: 121 LGLAVAIRFYTKQA 134

[Close]

BLAST of MS005615 vs. ExPASy TrEMBL
Match: A0A6J1IHM0 (cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111473649 PE=3 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 4.8e-70
Identity = 130/134 (97.01%), Postives = 133/134 (99.25%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHRDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 60
           MGSGEKVFTLKEVAEHNNH+DCWLIISGKVYDVTKFL+DHPGGDDVLLSATGKDATDDFE
Sbjct: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVTYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHSDNAREMMDQYYVGEIDGSTIPKKV YTPPKQPHYNQDKTSEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVAIRFYTKQA 135
           LGLAVA+RFYTKQA
Sbjct: 121 LGLAVALRFYTKQA 134

[Close]

BLAST of MS005615 vs. ExPASy TrEMBL
Match: A0A6J1F8A2 (cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111443010 PE=3 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 4.8e-70
Identity = 130/134 (97.01%), Postives = 133/134 (99.25%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHRDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 60
           MGSGEKVFTLKEVAEHNNH+DCWLIISGKVYDVTKFL+DHPGGDDVLLSATGKDATDDFE
Sbjct: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVTYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHSDNAREMMDQYYVGEIDGSTIPKKV YTPPKQPHYNQDKTSEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVAIRFYTKQA 135
           LGLAVA+RFYTKQA
Sbjct: 121 LGLAVALRFYTKQA 134

[Close]

BLAST of MS005615 vs. ExPASy TrEMBL
Match: A0A5D3D383 (Cytochrome b5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G002660 PE=3 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 6.2e-70
Identity = 131/134 (97.76%), Postives = 132/134 (98.51%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHRDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 60
           MGSGEKVFTLKEVAEHNNH+DCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE
Sbjct: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVTYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHSDNAREMMDQYYVGEID STIPKKV YTPPKQPHYNQDKTSEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSDNAREMMDQYYVGEIDASTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVAIRFYTKQA 135
           LGLAVAIRFYTKQA
Sbjct: 121 LGLAVAIRFYTKQA 134

[Close]

BLAST of MS005615 vs. ExPASy TrEMBL
Match: A0A0A0L7Y9 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G131950 PE=3 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 6.2e-70
Identity = 131/134 (97.76%), Postives = 132/134 (98.51%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHRDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 60
           MGSGEKVFTLKEVAEHNNH+DCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE
Sbjct: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVTYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHSDNAREMMDQYYVGEID STIPKKV YTPPKQPHYNQDKTSEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSDNAREMMDQYYVGEIDSSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVAIRFYTKQA 135
           LGLAVAIRFYTKQA
Sbjct: 121 LGLAVAIRFYTKQA 134

[Close]

BLAST of MS005615 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 235.3 bits (599), Expect = 2.8e-62
Identity = 110/132 (83.33%), Postives = 121/132 (91.67%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHRDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 60
           MG   K+FTL EV+EHN   DCW++I+GKVY+VTKFLEDHPGGDDVLLS+TGKDATDDFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVTYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHS++AREMM+QYYVGEID +TIPKKV YTPPKQPHYNQDKTSEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVAIRFYTK 133
           LGLAV IR YTK
Sbjct: 121 LGLAVGIRIYTK 132

[Close]

BLAST of MS005615 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 202.6 bits (514), Expect = 2.0e-52
Identity = 91/133 (68.42%), Postives = 113/133 (84.96%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHRDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 60
           M S  KV + +EV++HN  +DCWLIISGKVYDVT F++DHPGGD+VLLS+TGKDAT+DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVTYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHSD AR+MMD+Y++GEID S++P   TY  P+QP YNQDKT EFIIK+LQFLVP+ I
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPILI 120

Query: 121 LGLAVAIRFYTKQ 134
           LGLA+ +R YTK+
Sbjct: 121 LGLALVVRHYTKK 133

[Close]

BLAST of MS005615 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 188.7 bits (478), Expect = 3.0e-48
Identity = 86/134 (64.18%), Postives = 109/134 (81.34%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHRDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 60
           MG   KVFTL EV++H++ +DCW++I GKVYDVTKFL+DHPGGD+V+L++TGKDATDDFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVTYTPPKQPH--YNQDKTSEFIIKLLQFLVPL 120
           DVGHS  A+ M+D+YYVG+ID +T+P K  + PP        QDK+S+F+IKLLQFLVPL
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPL 120

Query: 121 AILGLAVAIRFYTK 133
            ILGLA  IR+YTK
Sbjct: 121 LILGLAFGIRYYTK 134

[Close]

BLAST of MS005615 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 132.5 bits (332), Expect = 2.5e-31
Identity = 61/129 (47.29%), Postives = 91/129 (70.54%), Query Frame = 0


Query: 7   VFTLKEVAEHNNHRDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFEDVGHSD 66
           + +  +VA+H    DCW++I GKVYD++ F+++HPGGD+VLL+ TGKDA+ DFEDV HS 
Sbjct: 4   LISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSK 63

Query: 67  NAREMMDQYYVGEIDGSTIPKKVTYTPPKQPHYNQDKTS--EFIIKLLQFLVPLAILGLA 126
           +A+E+M +Y +G++D ST+P    Y PP +      +T+  E   KLL +L+PL ILG+A
Sbjct: 64  DAKELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILGVA 123

Query: 127 VAIRFYTKQ 134
            A+RFY  +
Sbjct: 124 FALRFYNNK 132

[Close]

BLAST of MS005615 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 130.2 bits (326), Expect = 1.3e-30
Identity = 57/127 (44.88%), Postives = 93/127 (73.23%), Query Frame = 0


Query: 6   KVFTLKEVAEHNNHRDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFEDVGHS 65
           K+++++E A HN   DCW++I GKVYDV+ ++++HPGGDDVLL+  GKDATDDFED GHS
Sbjct: 6   KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 66  DNAREMMDQYYVGEIDGSTIPKKVTYTPPKQPHYNQDKTSEFIIKLLQ-----FLVPLAI 125
            +ARE+M++Y++GE+D S++P+      P+   Y +D+  + + KL       ++VP++I
Sbjct: 66  KDARELMEKYFIGELDESSLPE-----IPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSI 125

Query: 126 LGLAVAI 128
           + ++VA+
Sbjct: 126 ITISVAV 127

The following BLAST results are available for this feature:

BLAST of MS005615 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147043.1	3.1e-71	100.00	cytochrome b5-like [Momordica charantia]	[more]
XP_022936377.1	9.9e-70	97.01	cytochrome b5-like [Cucurbita moschata] >XP_022974878.1 cytochrome b5-like [Cucu...	[more]
XP_008438367.1	1.3e-69	97.76	PREDICTED: cytochrome b5 [Cucumis melo] >XP_038884390.1 cytochrome b5-like [Beni...	[more]
XP_004133998.1	1.3e-69	97.76	cytochrome b5 [Cucumis sativus] >KAE8650304.1 hypothetical protein Csa_009551 [C...	[more]
XP_023521594.1	1.3e-69	96.27	cytochrome b5-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS005615 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O48845	3.9e-61	83.33	Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1	[more]
P49098	3.7e-59	77.61	Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
P49099	1.8e-58	77.61	Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
P49100	2.9e-56	80.47	Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2	[more]
O04354	2.4e-55	76.74	Cytochrome b5 OS=Borago officinalis OX=13363 PE=2 SV=1	[more]

BLAST of MS005615 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D069	1.5e-71	100.00	cytochrome b5-like OS=Momordica charantia OX=3673 GN=LOC111016073 PE=3 SV=1	[more]
A0A6J1IHM0	4.8e-70	97.01	cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111473649 PE=3 SV=1	[more]
A0A6J1F8A2	4.8e-70	97.01	cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111443010 PE=3 SV=1	[more]
A0A5D3D383	6.2e-70	97.76	Cytochrome b5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G002660...	[more]
A0A0A0L7Y9	6.2e-70	97.76	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]

BLAST of MS005615 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32720.1	2.8e-62	83.33	cytochrome B5 isoform B	[more]
AT5G53560.1	2.0e-52	68.42	cytochrome B5 isoform E	[more]
AT5G48810.1	3.0e-48	64.18	cytochrome B5 isoform D	[more]
AT2G46650.1	2.5e-31	47.29	cytochrome B5 isoform C	[more]
AT1G26340.1	1.3e-30	44.88	cytochrome B5 isoform A	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 68..80 score: 40.09 coord: 40..54 score: 55.56 coord: 30..40 score: 70.52 coord: 55..62 score: 48.11
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..81 e-value: 2.3E-35 score: 133.5
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 9..80 e-value: 4.7E-28 score: 97.1
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 5..81 score: 30.670189
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 3..84 e-value: 3.7E-36 score: 125.0
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 6..91
None	No IPR available	PANTHER	PTHR19359:SF110	BNAC04G43300D PROTEIN	coord: 1..132
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 1..132
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 36..43

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS005615.1	MS005615.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0020037	heme binding