MS005444 (gene) Bitter gourd (TR) v1

Overview


Name	MS005444
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	beta-amyrin 11-oxidase-like
Location	scaffold641: 255550 .. 260029 (+)
RNA-Seq Expression	MS005444
Synteny	MS005444

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACTTTTGAGCAATTTTGGGGCCATTTTGGGACCTATTTTAGGGTTATACATCCTTGTCTTTGGGTTCATGAAGAAATTTAACGATATATGGTATCGTTTTAGGTTGGGAAAGAATGTTTACAATGCCCTCCCGCCCGGCGATATGGGATGGCCTCTTGTTGGCTCCTCTTTGTCGTTCTACCAAGCATTTAAAATCGGCGGCAATCCTAACTTTGTTATGGATGATCTCATTTCTAGGTATGTTGTCTCTCTTTGACTTCTTCTCTATCGTAGATAAGATTGTAAATGAAATGACTAGAGTTGTAAATCTACCTATTCGTTGCTTTAATAAGGATTCTGTAAATGTGTCAAATTACTGCAGAATGATTTTAACGATGTTCCATTAATCAGTTTAGAATCACTTTCAAAAGGTAGGGCTATAAAAGAAAGTAAAGTGTCATAGAAAAACAAAGTTCATATCGTTCAAGATGAACTTTCCATTTTGTGAGAAAAATAATGAAAGCTACGGCAGGCAGCCGGCGGCTGCCGCTGGTTTTTCTTAAGCTGCTGAGCAAGTNTTTATTTTTTAATTTTTTTGTTCATTATGCGGTTGTATTAATTTTTTATTTATTTTTTTTTTTCTAAAGTCAATAGGTTGATCGGTTGCAAAATTTTGGAAGAAAATACCTCTATAAATTATATCAATTGTAACTTAAAACTTTTTAAATTTGGTAATAAATTTTAAGTTAAAAAATAGATTTGGTCCATGTAATTAGTAATAATTTAGTCTCTGAACTTTAGTTTGTAACAATTTAGTATTTGTACTTCTCTAAAGTAACAATTTAGTCCCTGAATTTTTACTTGTAATGATTTAGTCGTTGTAGTTTAAAATTTGTAACATTTTAGTCTTTATCATAAGAAATTTTATTAAAATGAAGCGTCAAATTTTATAATATAACAATTTAATATTTCTAATTTATAAACATATTTGGTCCCTCTTTAGTTTATTAATCTGTATATATAAGAAACTTAACAAAAAGAATTTCACAATAAAGACTAAATCGTTACCTACTTTAAATTACAAAGACTAAATTATTACATATTTAAAGTTCAAGAACTAAATTGTTACCAATTTGAAAGTATGTGGATTAAATAGTTACAAATTAAAATTTAGGGACTAAATTGTTACTTTAGCTAAAGTACCTTGACCAAAAATATTTTTTAACATAAATTTTAATAACTATTACAAGTTACCACTTTAATTTTTTATAATTTTTTTTCTAATATGTAAAAAACCTTTAAAAACTTACCACTTTAATGAAAATCTATATCAACAAATTAAAACATGTCAACTATTTTTCAAATGAAAAGGTAAAATTTTCAATACCTTTTAAAGTTAAAGGTGTATTTTGCAAAAACTTATCTCAGTTTAAATTGCAATTTGATAAATTTGAAGTTCATGGTTAAAATGGATAAGTTCAAGGTAGAAATTTATATTTCCAATTAAAAAAAAAAAAGTGATACAACTTATCTTAGTTGTGCTCTTAGTTTCCTTTTCATTAATTCCATACAGAAAATATTGTTTCTCGAATTTTTTTTCATTTAATTTTTATAATATTTTGGAAATGCATACTTGAAATTGTGGCCCTTTTGACATAAATTCTTAGATCCGTCTTGAAATAAATTGTTAATAATATGAACAATTTATTAAATATACAGCAACCATCTTAAGTTTTTCTTGTTTATACAACATTTGGATTATTTGTAATGTAATTTTTTTTTTCCGAATTTAAAGTAACGTTATTTCCTTCTTCATTAACTTTAATAGCTACTTGTTGGAGTCTCACCACGAAACAGAGCAATTAGAATTATGAACAATTAATTAAATTTGTAATTTGTATGCTTTTTAATTTAGCAGCAATTTTATAAAATTAAAACTTTCCTGAAGTTATTTCCTTCCTAATTACCAAGAAATAAGATAGAGTACGTCATAAGAATATTAACATATAATTCAATATCTATAACCTTTCGATTTAAGTTATTGAACTCAGCTGTTATAATTTAATGTGGTACAAAGTAAAGGGGCTGTGTGTTTGAATACATGTAATGTCATTTCCTTCTTAATTACTAATACTTATGCATTTCCTTCTTAATTACTAATACTTATGGTTACTAAATTACTAAACGTTCCTTAGCCTATTAAATTTAAATTGTTTTTCAAGTATAGGTGTCAATTAATCCTAAGCTAGGTTCATGTTAACTTTAATATACATATTTAAATACATGTTTGTTCCTGATGACAGATTTGGAAGAGTTAGGATGTACAAAAGTCACCTATACGGTTTTCCAGTAATCATCGTGACAGATCCAGAAGTATGCCGACGCATATACTTAGACGACGAACATTTCAAGTTGAACTATCCAAAGTCAGTGAAACTATTACAAGCAGATGGTTCACTATTGAAGGTCGACTACAAAATTGTGCACCGCTTGATGGCAGCTCCGATAAACGGCGTCGAGGCGTTGTCTCGGTATGTCGAGTTCATCGAAGATGCGGTGGTGGAGGGTTTGGAGGAATGGGGCAGCATGAAAGGCCCAGTGCCGTTGCTTCATGAATTCAAACGCCTCACCTTCAAGATCATTATAGGGGTTTTCTTGGGGCCTGTTTTCACTGATTCTGAAGTAGCAGAACTTGAGAGTTTGTACGCAGAGATCGGACCAGCAATCATGTCCATTTTCCCTTATAACTTGCCAGGATTCAGTTACCGTCGAGCACTCAAAGTACGTACATCATCCATTAATTCGTGTTAATCGTAATTACTAATCTGCTATCAATATTCATGGGATTCTATCTCAAAAGCACTTATCTTTTCAATGCGAGATCCTTAACATGTAGCACGTATTTTTTTATAGGCGAGAATTAAGGTGGAAGATATCGTTCGGCCCGTGATACAAAAGAGGAAAAAGGCATTAGAAAACAACGTGGATTTAGAAGAGACATGTCAATTGGACGCAGTGATTAAGGCAACGGATAAGAATGGGGAGAGAGTATTCAATGACAATGCAATTATTTGCATGCTACTTGGACTGCTATATGCTGGTTATCATACCTCAGCTCATGGTGCTCAGTGGACAATGGTTCATGTTTCAGAAAACCCAGATGTCTACCAAAAGGCCAAGGTCTCATCATTTGATCTCCCAAATTAAACACTTGATAATATAATATAATTAACAAGTTGCTCATGATTTGGTGATGGTTTTTTACAGGAAGAGCAAGAGATGATCATCAAGCAAAGACCACCTACACAAAAAGGATTGAATTTTAAAGAAATTAAACAAATGAAGTACACCGCAAAGGTACTATATATTGTGTTCTTTATCATCATATGTAACGTTGATGAACACATGCTTCTAATTAACTCATCCAGTAACGCATGTCACAAATATCATTAAGCTAAAGCAATCATTAATATGGTTGGTTTCTAGGAGAACACATGAATATCAAATCTCCAGAGAAGATATGATTAGGATCAAAAAGTTTGAGTCAAATAAAACATAACATGAGATAGATTAGTAGTTTGGTATCCTCTTCAAATGCATCTAAAATGATGTTTAGACCATCCGTTAAAGCTTGAATTAAGAGTTTGGTAACAAATAAGAAGAGCTAAACATCTCATTCCATCTTTATTACTTTTATTTGAGTGAAATATATACCATTCAACTTTTATTATTACTTTTTTAATTATCAATTTTTGTTTTCAGATCATCAATGAAGTATTAAGACTCAATAACATTGTGTTCACAAATTTTCGCATGGCAACCAAAGACGTAAATGTCAATGGTAAGTGATTCTATTTATTCCATTATTATCATTTATATATGGAAAATACTTTGCTCCTTAACGGTGAGACCTCACCACCATTAAAAAATTGACAACTCATTCATTTTTATAAAAAAAAATTACTGTTTTTACATTTCTCCATCTTAATGGTCAAATATTTAAGGATGGTAACGACAAGATTGCTCCTCACCATTAAGGAGCCAACATTAACTATTGAATTATTGTATATATAATTAACTCAATGTCAGATTGAATTAATTATTTACTCATTGATAATAATTTTTTTTAATTACTGTTTTCTTTCTTCTTTACTTATCATAGGTTATACTATACCAAAAGGATGGATTGCAGCAATGTGGATTAGAGGCGTACACATGGACGATAAAATATATGAAAATCCATTGGAATTTAATCCTTCAAGATGGGATGTAAGTCTTAAGATCCCATTCAATATATATATGAATTCATTTATTTGAAAATTATGTCTGCTTCAGTGATGTTTAATTTTCTGTTTTTGTAAATGATTGTGCAGAACTATACTCCAAAACCAGGAGAGTATATCCCATTTGGAATTGGAAGCAGGTTTTGCCCTGGAAATGAACTTGCAAAGCTTGAAATGACCGTTTTGCTTCACCATTTTCTTCTGGGCTACAGG

mRNA sequence

ATGGAACTTTTGAGCAATTTTGGGGCCATTTTGGGACCTATTTTAGGGTTATACATCCTTGTCTTTGGGTTCATGAAGAAATTTAACGATATATGGTATCGTTTTAGGTTGGGAAAGAATGTTTACAATGCCCTCCCGCCCGGCGATATGGGATGGCCTCTTGTTGGCTCCTCTTTGTCGTTCTACCAAGCATTTAAAATCGGCGGCAATCCTAACTTTGTTATGGATGATCTCATTTCTAGATTTGGAAGAGTTAGGATGTACAAAAGTCACCTATACGGTTTTCCAGTAATCATCGTGACAGATCCAGAAGTATGCCGACGCATATACTTAGACGACGAACATTTCAAGTTGAACTATCCAAAGTCAGTGAAACTATTACAAGCAGATGGTTCACTATTGAAGGTCGACTACAAAATTGTGCACCGCTTGATGGCAGCTCCGATAAACGGCGTCGAGGCGTTGTCTCGGTATGTCGAGTTCATCGAAGATGCGGTGGTGGAGGGTTTGGAGGAATGGGGCAGCATGAAAGGCCCAGTGCCGTTGCTTCATGAATTCAAACGCCTCACCTTCAAGATCATTATAGGGGTTTTCTTGGGGCCTGTTTTCACTGATTCTGAAGTAGCAGAACTTGAGAGTTTGTACGCAGAGATCGGACCAGCAATCATGTCCATTTTCCCTTATAACTTGCCAGGATTCAGTTACCGTCGAGCACTCAAAGCGAGAATTAAGGTGGAAGATATCGTTCGGCCCGTGATACAAAAGAGGAAAAAGGCATTAGAAAACAACGTGGATTTAGAAGAGACATGTCAATTGGACGCAGTGATTAAGGCAACGGATAAGAATGGGGAGAGAGTATTCAATGACAATGCAATTATTTGCATGCTACTTGGACTGCTATATGCTGGTTATCATACCTCAGCTCATGGTGCTCAGTGGACAATGGTTCATGTTTCAGAAAACCCAGATGTCTACCAAAAGGCCAAGGAAGAGCAAGAGATGATCATCAAGCAAAGACCACCTACACAAAAAGGATTGAATTTTAAAGAAATTAAACAAATGAAGTACACCGCAAAGATCATCAATGAAGTATTAAGACTCAATAACATTGTGTTCACAAATTTTCGCATGGCAACCAAAGACGTAAATGTCAATGGTTATACTATACCAAAAGGATGGATTGCAGCAATGTGGATTAGAGGCGTACACATGGACGATAAAATATATGAAAATCCATTGGAATTTAATCCTTCAAGATGGGATAACTATACTCCAAAACCAGGAGAGTATATCCCATTTGGAATTGGAAGCAGGTTTTGCCCTGGAAATGAACTTGCAAAGCTTGAAATGACCGTTTTGCTTCACCATTTTCTTCTGGGCTACAGG

Coding sequence (CDS)

ATGGAACTTTTGAGCAATTTTGGGGCCATTTTGGGACCTATTTTAGGGTTATACATCCTTGTCTTTGGGTTCATGAAGAAATTTAACGATATATGGTATCGTTTTAGGTTGGGAAAGAATGTTTACAATGCCCTCCCGCCCGGCGATATGGGATGGCCTCTTGTTGGCTCCTCTTTGTCGTTCTACCAAGCATTTAAAATCGGCGGCAATCCTAACTTTGTTATGGATGATCTCATTTCTAGATTTGGAAGAGTTAGGATGTACAAAAGTCACCTATACGGTTTTCCAGTAATCATCGTGACAGATCCAGAAGTATGCCGACGCATATACTTAGACGACGAACATTTCAAGTTGAACTATCCAAAGTCAGTGAAACTATTACAAGCAGATGGTTCACTATTGAAGGTCGACTACAAAATTGTGCACCGCTTGATGGCAGCTCCGATAAACGGCGTCGAGGCGTTGTCTCGGTATGTCGAGTTCATCGAAGATGCGGTGGTGGAGGGTTTGGAGGAATGGGGCAGCATGAAAGGCCCAGTGCCGTTGCTTCATGAATTCAAACGCCTCACCTTCAAGATCATTATAGGGGTTTTCTTGGGGCCTGTTTTCACTGATTCTGAAGTAGCAGAACTTGAGAGTTTGTACGCAGAGATCGGACCAGCAATCATGTCCATTTTCCCTTATAACTTGCCAGGATTCAGTTACCGTCGAGCACTCAAAGCGAGAATTAAGGTGGAAGATATCGTTCGGCCCGTGATACAAAAGAGGAAAAAGGCATTAGAAAACAACGTGGATTTAGAAGAGACATGTCAATTGGACGCAGTGATTAAGGCAACGGATAAGAATGGGGAGAGAGTATTCAATGACAATGCAATTATTTGCATGCTACTTGGACTGCTATATGCTGGTTATCATACCTCAGCTCATGGTGCTCAGTGGACAATGGTTCATGTTTCAGAAAACCCAGATGTCTACCAAAAGGCCAAGGAAGAGCAAGAGATGATCATCAAGCAAAGACCACCTACACAAAAAGGATTGAATTTTAAAGAAATTAAACAAATGAAGTACACCGCAAAGATCATCAATGAAGTATTAAGACTCAATAACATTGTGTTCACAAATTTTCGCATGGCAACCAAAGACGTAAATGTCAATGGTTATACTATACCAAAAGGATGGATTGCAGCAATGTGGATTAGAGGCGTACACATGGACGATAAAATATATGAAAATCCATTGGAATTTAATCCTTCAAGATGGGATAACTATACTCCAAAACCAGGAGAGTATATCCCATTTGGAATTGGAAGCAGGTTTTGCCCTGGAAATGAACTTGCAAAGCTTGAAATGACCGTTTTGCTTCACCATTTTCTTCTGGGCTACAGG

Protein sequence

MELLSNFGAILGPILGLYILVFGFMKKFNDIWYRFRLGKNVYNALPPGDMGWPLVGSSLSFYQAFKIGGNPNFVMDDLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVEDIVRPVIQKRKKALENNVDLEETCQLDAVIKATDKNGERVFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKIINEVLRLNNIVFTNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDNYTPKPGEYIPFGIGSRFCPGNELAKLEMTVLLHHFLLGYR

Homology

[Close]

BLAST of MS005444 vs. NCBI nr
Match: XP_022157153.1 (beta-amyrin 11-oxidase-like [Momordica charantia] >BBI21929.1 cytochrome P450 monooxygenase [Momordica charantia])

HSP 1 Score: 929.9 bits (2402), Expect = 8.7e-267
Identity = 451/462 (97.62%), Postives = 458/462 (99.13%), Query Frame = 0


Query: 1   MELLSNFGAILGPILGLYILVFGFMKKFNDIWYRFRLGKNVYNALPPGDMGWPLVGSSLS 60
           MELLSNFGAILG +LGLYILVFGF++KFNDIWYRF+LGKNVYNALPPGDMGWPLVGSSLS
Sbjct: 1   MELLSNFGAILGALLGLYILVFGFLRKFNDIWYRFKLGKNVYNALPPGDMGWPLVGSSLS 60

Query: 61  FYQAFKIGGNPNFVMDDLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNY 120
           FYQAFKIGG+PN VMD+LISRFGRVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNY
Sbjct: 61  FYQAFKIGGDPNAVMDNLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNY 120

Query: 121 PKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPV 180
           PKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPV
Sbjct: 121 PKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPV 180

Query: 181 PLLHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALK 240
           PLLHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALK
Sbjct: 181 PLLHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALK 240

Query: 241 ARIKVEDIVRPVIQKRKKALENNVDLEETCQLDAVIKATDKNGERVFNDNAIICMLLGLL 300
           ARIKVE+IVRPVIQ RKKALENNVDLEETCQLDAVIKATDKNGERVFNDNAIICMLLGLL
Sbjct: 241 ARIKVENIVRPVIQNRKKALENNVDLEETCQLDAVIKATDKNGERVFNDNAIICMLLGLL 300

Query: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKI 360
           YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPP QKGLNFKEIKQMKYTAKI
Sbjct: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPPNQKGLNFKEIKQMKYTAKI 360

Query: 361 INEVLRLNNIVFTNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRW 420
           INEVLRLNNIVFTNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRW
Sbjct: 361 INEVLRLNNIVFTNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRW 420

Query: 421 DNYTPKPGEYIPFGIGSRFCPGNELAKLEMTVLLHHFLLGYR 463
           DNYTPKPGEYIPFGIGSRFCPGNELAKLEMTVLLHHFLLGYR
Sbjct: 421 DNYTPKPGEYIPFGIGSRFCPGNELAKLEMTVLLHHFLLGYR 462

[Close]

BLAST of MS005444 vs. NCBI nr
Match: BBI21930.1 (cytochrome P450 monooxygenase [Momordica charantia])

HSP 1 Score: 786.6 bits (2030), Expect = 1.2e-223
Identity = 366/462 (79.22%), Postives = 420/462 (90.91%), Query Frame = 0


Query: 1   MELLSNFGAILGPILGLYILVFGFMKKFNDIWYRFRLGKNVYNALPPGDMGWPLVGSSLS 60
           ME+L+NF A+LG  +GLY  VFGF++ FN++WY  RLG  +YNALPPG MGWPL+GSSLS
Sbjct: 1   MEILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLS 60

Query: 61  FYQAFKIGGNPNFVMDDLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNY 120
           FY+AFKIGG+PN +++ L+SR+GRVRMYKSHLYG PVIIVTDPEVCRR+YLDDE FKL+Y
Sbjct: 61  FYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSY 120

Query: 121 PKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPV 180
           PKSV +LQ D SLLK+DYKIVHRLMAAPING EALS+Y+ FIE+AVV+GLEEWGS+KGPV
Sbjct: 121 PKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPV 180

Query: 181 PLLHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALK 240
            LL EFKRLTFK+I GVFLGP FTDSE+A+LE+LYAEIG AI+SIFPY+LPGFSYRRALK
Sbjct: 181 ELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALK 240

Query: 241 ARIKVEDIVRPVIQKRKKALENNVDLEETCQLDAVIKATDKNGERVFNDNAIICMLLGLL 300
           AR KVE+IVRPVI++R++ LE N +LEETCQ+DAVIKATDKNGE++FNDNAIICMLLGLL
Sbjct: 241 ARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIICMLLGLL 300

Query: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKI 360
           YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRP TQKGLNFKEIKQMKY  K+
Sbjct: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKV 360

Query: 361 INEVLRLNNIVFTNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRW 420
           INE+LRLNNIVFT FR+AT DVNVNGY IPKGW   +W RGVHMD +IY+NPLEF+PSRW
Sbjct: 361 INEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRW 420

Query: 421 DNYTPKPGEYIPFGIGSRFCPGNELAKLEMTVLLHHFLLGYR 463
           DNY PKPGE+IPFG+GSR+CPGNELA+LE+ +LLHHFLLGYR
Sbjct: 421 DNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 462

[Close]

BLAST of MS005444 vs. NCBI nr
Match: XP_022157134.1 (beta-amyrin 11-oxidase-like isoform X1 [Momordica charantia])

HSP 1 Score: 785.0 bits (2026), Expect = 3.5e-223
Identity = 365/462 (79.00%), Postives = 419/462 (90.69%), Query Frame = 0


Query: 1   MELLSNFGAILGPILGLYILVFGFMKKFNDIWYRFRLGKNVYNALPPGDMGWPLVGSSLS 60
           ME+L+NF A+LG  +GLY  VFGF++ FN++WY  RLG  +YNALPPG MGWPL+GSSLS
Sbjct: 1   MEILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLS 60

Query: 61  FYQAFKIGGNPNFVMDDLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNY 120
           FY+AFKIGG+PN +++ L+SR+GRVRMYKSHLYG PVIIVTDPEVCRR+YLDDE FKL+Y
Sbjct: 61  FYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSY 120

Query: 121 PKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPV 180
           PKSV +LQ D SLLK+DYKIVHRLMAAPING EALS+Y+ FIE+AVV+GLEEWGS+KGPV
Sbjct: 121 PKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPV 180

Query: 181 PLLHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALK 240
            LL EFKRLTFK+I GVFLGP FTDSE+A+LE+LYAEIG AI+SIFPY+LPGFSYRRALK
Sbjct: 181 ELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALK 240

Query: 241 ARIKVEDIVRPVIQKRKKALENNVDLEETCQLDAVIKATDKNGERVFNDNAIICMLLGLL 300
           AR KVE+IVRPVI++R++ LE N +LEETCQ+DAVIKATDKNGE++FNDNAI CMLLGLL
Sbjct: 241 ARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLL 300

Query: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKI 360
           YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRP TQKGLNFKEIKQMKY  K+
Sbjct: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKV 360

Query: 361 INEVLRLNNIVFTNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRW 420
           INE+LRLNNIVFT FR+AT DVNVNGY IPKGW   +W RGVHMD +IY+NPLEF+PSRW
Sbjct: 361 INEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRW 420

Query: 421 DNYTPKPGEYIPFGIGSRFCPGNELAKLEMTVLLHHFLLGYR 463
           DNY PKPGE+IPFG+GSR+CPGNELA+LE+ +LLHHFLLGYR
Sbjct: 421 DNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 462

[Close]

BLAST of MS005444 vs. NCBI nr
Match: XP_022157141.1 (beta-amyrin 11-oxidase-like isoform X2 [Momordica charantia])

HSP 1 Score: 719.2 bits (1855), Expect = 2.3e-203
Identity = 336/413 (81.36%), Postives = 381/413 (92.25%), Query Frame = 0


Query: 50  MGWPLVGSSLSFYQAFKIGGNPNFVMDDLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRI 109
           MGWPL+GSSLSFY+AFKIGG+PN +++ L+SR+GRVRMYKSHLYG PVIIVTDPEVCRR+
Sbjct: 1   MGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRV 60

Query: 110 YLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVEFIEDAVVEG 169
           YLDDE FKL+YPKSV +LQ D SLLK+DYKIVHRLMAAPING EALS+Y+ FIE+AVV+G
Sbjct: 61  YLDDERFKLSYPKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKG 120

Query: 170 LEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYN 229
           LEEWGS+KGPV LL EFKRLTFK+I GVFLGP FTDSE+A+LE+LYAEIG AI+SIFPY+
Sbjct: 121 LEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYD 180

Query: 230 LPGFSYRRALKARIKVEDIVRPVIQKRKKALENNVDLEETCQLDAVIKATDKNGERVFND 289
           LPGFSYRRALKAR KVE+IVRPVI++R++ LE N +LEETCQ+DAVIKATDKNGE++FND
Sbjct: 181 LPGFSYRRALKARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFND 240

Query: 290 NAIICMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPPTQKGLNFK 349
           NAI CMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRP TQKGLNFK
Sbjct: 241 NAIFCMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFK 300

Query: 350 EIKQMKYTAKIINEVLRLNNIVFTNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIY 409
           EIKQMKY  K+INE+LRLNNIVFT FR+AT DVNVNGY IPKGW   +W RGVHMD +IY
Sbjct: 301 EIKQMKYATKVINEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIY 360

Query: 410 ENPLEFNPSRWDNYTPKPGEYIPFGIGSRFCPGNELAKLEMTVLLHHFLLGYR 463
           +NPLEF+PSRWDNY PKPGE+IPFG+GSR+CPGNELA+LE+ +LLHHFLLGYR
Sbjct: 361 QNPLEFDPSRWDNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 413

[Close]

BLAST of MS005444 vs. NCBI nr
Match: XP_022952147.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])

HSP 1 Score: 571.2 bits (1471), Expect = 7.9e-159
Identity = 264/462 (57.14%), Postives = 350/462 (75.76%), Query Frame = 0


Query: 1   MELLSNFGAILGPILGLYILVFGFMKKFNDIWYRFRLGKNVYNALPPGDMGWPLVGSSLS 60
           ME++ N   I+G +LG+Y+    F++K N+IWY  +LG+  Y +LPPGDMGWPL+GSS S
Sbjct: 1   MEMI-NVCTIIGAVLGVYM----FVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFS 60

Query: 61  FYQAFKIGGNPNFVMDDLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNY 120
           FY AF +GG+P+  +  L+SR GRV MYKSHLYG P IIVTDPE+CRRIYLDDE FK NY
Sbjct: 61  FYNAFTVGGDPDSFIYHLLSRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDEIFKPNY 120

Query: 121 PKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPV 180
           PKSVK+L+ +G   + D+K  +R+MAAP+NG E LS YV+FI+  + +GLEEW SMK P+
Sbjct: 121 PKSVKILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPI 180

Query: 181 PLLHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALK 240
            LL E   L FK+I  +FLG     S +AELE+LY ++GPAI+SI PY++PG  Y RALK
Sbjct: 181 ELLGEIGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDVPGLVYHRALK 240

Query: 241 ARIKVEDIVRPVIQKRKKALENNVDLEETCQLDAVIKATDKNGERVFNDNAIICMLLGLL 300
           AR K+E+++  VI+++++ALE     E   Q+D +I A D+NG +++++N II +LLGLL
Sbjct: 241 ARKKIENVLECVIEEKRRALEQKETREVHSQMDKLIMARDENGMKLYDNNTIIDLLLGLL 300

Query: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKI 360
           +AG+HT A+ A W +VH+SE P ++ KAKEEQE I+KQRP TQKGL F+EIKQMKY  K 
Sbjct: 301 HAGHHTPAYAAMWALVHISEKPHIFHKAKEEQESILKQRPSTQKGLTFQEIKQMKYLMKF 360

Query: 361 INEVLRLNNIVFTNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRW 420
           INE+LR N IV  +FR A  D+N+N Y IPKGW   +W R VHMD ++Y NP EF+PSRW
Sbjct: 361 INELLRRNTIVVASFRKAKTDININDYIIPKGWSVLIWTRAVHMDPQLYSNPEEFDPSRW 420

Query: 421 DNYTPKPGEYIPFGIGSRFCPGNELAKLEMTVLLHHFLLGYR 463
           +N++PKPG +IPFGIG+RFCPG+EL KLE+T+LLHHF+L Y+
Sbjct: 421 ENHSPKPGTFIPFGIGTRFCPGSELTKLEITILLHHFILNYK 457

[Close]

BLAST of MS005444 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 2.3e-108
Identity = 198/454 (43.61%), Postives = 301/454 (66.30%), Query Frame = 0


Query: 14  ILGLYILVFGFMKKFNDIWYRFRLGKNVYNALPPGDMGWPLVGSSLSFYQAFKIGGNPNF 73
           +L  YI    F++  N  +Y  +L +  +  LPPGDMGWPL+G  LSF + F   G+P+ 
Sbjct: 13  LLVCYIFGSKFVRNLNGWYYDVKLRRKEH-PLPPGDMGWPLIGDLLSFIKDFS-SGHPDS 72

Query: 74  VMDDLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSL 133
            +++L+ ++GR  +YK+HL+G P IIV +P++CRR+  DD +FKL YPKS+K L     +
Sbjct: 73  FINNLVLKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKSIKELARCRPM 132

Query: 134 LKV---DYKIVHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLT 193
           + V   ++++  RL+ +PI G +AL+ Y+E +E+ V+  LEE  SMK PV LL E K+++
Sbjct: 133 IDVSNAEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPVELLKEMKKVS 192

Query: 194 FKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVEDIVR 253
           FK I+ VF+G    D  + ++ S + ++   + SI P N+PGF++ +AL+AR K+  IV+
Sbjct: 193 FKAIVHVFMGSSNQDI-IKKIGSSFTDLYNGMFSI-PINVPGFTFHKALEARKKLAKIVQ 252

Query: 254 PVIQKRKKALENNVD--LEETCQLDAVIKATDKNGERVFNDNAIICMLLGLLYAGYHTSA 313
           PV+ +R+  +EN      +    +D +++  D+NG R   D  I  +L+GLL+AG+ ++A
Sbjct: 253 PVVDERRLMIENGPQEGSQRKDLIDILLEVKDENG-RKLEDEDISDLLIGLLFAGHESTA 312

Query: 314 HGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKIINEVLRLN 373
               W++ +++++P + +KAKEEQE I + R  +QK L+ KEIKQM Y +++I+E LR  
Sbjct: 313 TSLMWSITYLTQHPHILKKAKEEQEEITRTRFSSQKQLSLKEIKQMVYLSQVIDETLRCA 372

Query: 374 NIVFTNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDNYTPKPG 433
           NI F  FR AT DVN+NGY IPKGW   +W R +HMD + Y NP EFNPSRWD+Y  K G
Sbjct: 373 NIAFATFREATADVNINGYIIPKGWRVLIWARAIHMDSEYYPNPEEFNPSRWDDYNAKAG 432

Query: 434 EYIPFGIGSRFCPGNELAKLEMTVLLHHFLLGYR 463
            ++PFG GSR CPG +LAKLE+++ LH+FL  YR
Sbjct: 433 TFLPFGAGSRLCPGADLAKLEISIFLHYFLRNYR 461

[Close]

BLAST of MS005444 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 376.7 bits (966), Expect = 3.7e-103
Identity = 193/452 (42.70%), Postives = 287/452 (63.50%), Query Frame = 0


Query: 14  ILGLYILVFGFMKKFNDIWYRFRLGKNVYNALPPGDMGWPLVGSSLSFYQAFKIGGNPNF 73
           ILGL++L +  +K+ N   Y  +LG+  +  LPPGD+GWP++G+  SF +AFK   +P  
Sbjct: 15  ILGLFVLKW-VLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFKT-SDPES 74

Query: 74  VMDDLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSL 133
            +   I+R+GR  +YK+H++G+P ++VT PE CRR+  DD+ F + +PKS   L    S 
Sbjct: 75  FIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSF 134

Query: 134 LKV---DYKIVHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLT 193
           + +   ++K + RL +AP+NG EALS Y++FIE+ V   LE+W  M G +  L   ++LT
Sbjct: 135 VGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKM-GEIEFLSHLRKLT 194

Query: 194 FKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVEDIVR 253
           FK+I+ +FL    ++  +  LE  Y  +   + ++   NLPGF+Y RALKAR K+    +
Sbjct: 195 FKVIMYIFLSSE-SEHVMDSLEREYTNLNYGVRAM-GINLPGFAYHRALKARKKLVAAFQ 254

Query: 254 PVIQKRKKALENNVDLEETCQLDAVIKATDKNGERVFNDNAIICMLLGLLYAGYHTSAHG 313
            ++  R+   + N+       LD +I   D+NG RV +D  II +LL  L AG+ +S H 
Sbjct: 255 SIVTNRRNQRKQNISSNRKDMLDNLIDVKDENG-RVLDDEEIIDLLLMYLNAGHESSGHL 314

Query: 314 AQWTMVHVSENPDVYQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKIINEVLRLNNI 373
             W  + + E+P + QKAKEEQE I+K+R P QK L  KE ++M Y +++I+E LR+   
Sbjct: 315 TMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITF 374

Query: 374 VFTNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDNYTPKPGEY 433
             T FR A  DV ++GY IPKGW    W R VH+D +IY +P +F+PSRW+ YTPK G +
Sbjct: 375 SLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTF 434

Query: 434 IPFGIGSRFCPGNELAKLEMTVLLHHFLLGYR 463
           +PFG+GS  CPGN+LAKLE+++ LHHFLL YR
Sbjct: 435 LPFGLGSHLCPGNDLAKLEISIFLHHFLLKYR 458

[Close]

BLAST of MS005444 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 1.2e-101
Identity = 187/450 (41.56%), Postives = 281/450 (62.44%), Query Frame = 0


Query: 19  ILVFGFMKKFNDIWYRF-RLGKNVYNALPPGDMGWPLVGSSLSFYQAFKIGGNPNFVMDD 78
           +LV   +++ +D W R   LG    + LPPG+MGWP+VGS  +F +AFK  GNP+  +  
Sbjct: 29  VLVDAVVRRAHD-WVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAFK-SGNPDAFIAS 88

Query: 79  LISRFGRVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKV- 138
            I RFGR  +Y++ ++  P I+   PE C+++ +DDE F   +PK+   L    S + + 
Sbjct: 89  FIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATVTLIGPKSFVNMS 148

Query: 139 --DYKIVHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMK-GPVPLLHEFKRLTFKI 198
             D++ + +L AAPING +AL+ Y+ FI+  VV  L  W S + G V  L E +R+TFKI
Sbjct: 149 YDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEFLTELRRMTFKI 208

Query: 199 IIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVEDIVRPVI 258
           I+ +F+     D+ +  LE  Y ++    M     NLPGF+Y RAL+AR K+  +++ V+
Sbjct: 209 IVQIFMSGA-DDATMEALERSYTDLNYG-MRAMAINLPGFAYYRALRARRKLVSVLQGVL 268

Query: 259 QKRKKALENNVDLEETC-QLDAVIKATDKNGERVFNDNAIICMLLGLLYAGYHTSAHGAQ 318
             R+ A             +D +I+A D+ G R+ +D  I+ +L+  L AG+ +S H   
Sbjct: 269 DGRRAAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDE-IVDVLIMYLNAGHESSGHITM 328

Query: 319 WTMVHVSENPDVYQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKIINEVLRLNNIVF 378
           W  V + ENPD++ +AK EQE I++  P TQ GL  ++ K+M + +++++E LR  NI F
Sbjct: 329 WATVFLQENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQVVDETLRCVNISF 388

Query: 379 TNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDNYTPKPGEYIP 438
            +FR AT+D+ VNGY IPKGW   +W R VHMDD++Y +P  FNPSRW+   PK G ++P
Sbjct: 389 VSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRWEGPPPKAGTFLP 448

Query: 439 FGIGSRFCPGNELAKLEMTVLLHHFLLGYR 463
           FG+G+R CPGN+LAKLE++V LHHFLLGY+
Sbjct: 449 FGLGARLCPGNDLAKLEISVFLHHFLLGYK 473

[Close]

BLAST of MS005444 vs. ExPASy Swiss-Prot
Match: Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 3.9e-100
Identity = 182/435 (41.84%), Postives = 273/435 (62.76%), Query Frame = 0


Query: 32  WYR-FRLGKNVYNALPPGDMGWPLVGSSLSFYQAFKIGGNPNFVMDDLISRFGRVRMYKS 91
           WYR   LG      LPPG+MGWPLVG   +F +AFK  G P+  +   + RFGR  +Y+S
Sbjct: 58  WYREAALGAARRARLPPGEMGWPLVGGMWAFLRAFK-SGKPDAFIASFVRRFGRTGVYRS 117

Query: 92  HLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSLLKVDY---KIVHRLMAA 151
            ++  P ++VT  E C+++ +DD+ F   +PK+   L    S + + Y   + + +L AA
Sbjct: 118 FMFSSPTVLVTTAEGCKQVLMDDDAFVTGWPKATVALVGPRSFVAMPYDEHRRIRKLTAA 177

Query: 152 PINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGPVFTDSE 211
           PING +AL+ Y+ FI+  V   L  W    G V  L E +R+TFKII+ +FLG     + 
Sbjct: 178 PINGFDALTGYLPFIDRTVTSSLRAWADHGGSVEFLTELRRMTFKIIVQIFLGGA-DQAT 237

Query: 212 VAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVEDIVRPVIQKRKKALENNVDLE 271
              LE  Y E+    M     NLPGF+YR AL+AR ++  +++ V+ +R+ A    V   
Sbjct: 238 TRALERSYTELNYG-MRAMAINLPGFAYRGALRARRRLVAVLQGVLDERRAARAKGVSGG 297

Query: 272 ETCQLDAVIKATDKNGERVFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQK 331
               +D +I+A D+ G R  +D+ II +L+  L AG+ +S H   W  V + ENPD++ +
Sbjct: 298 GVDMMDRLIEAQDERG-RHLDDDEIIDVLVMYLNAGHESSGHITMWATVFLQENPDMFAR 357

Query: 332 AKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKIINEVLRLNNIVFTNFRMATKDVNVNGY 391
           AK EQE I++  P +Q+GL  ++ ++M+Y +++I+E LRL NI F +FR AT+DV VNGY
Sbjct: 358 AKAEQEAIMRSIPSSQRGLTLRDFRKMEYLSQVIDETLRLVNISFVSFRQATRDVFVNGY 417

Query: 392 TIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDNYTPKPGEYIPFGIGSRFCPGNELAK 451
            IPKGW   +W R VHMD ++Y +P +F+PSRW+ ++P+ G ++ FG+G+R CPGN+LAK
Sbjct: 418 LIPKGWKVQLWYRSVHMDPQVYPDPTKFDPSRWEGHSPRAGTFLAFGLGARLCPGNDLAK 477

Query: 452 LEMTVLLHHFLLGYR 463
           LE++V LHHFLLGY+
Sbjct: 478 LEISVFLHHFLLGYK 488

[Close]

BLAST of MS005444 vs. ExPASy Swiss-Prot
Match: Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 2.0e-96
Identity = 181/462 (39.18%), Postives = 273/462 (59.09%), Query Frame = 0


Query: 9   AILGPILGLYILVFGFMKKFNDIWY-RFRLGKNVYNALPPGDMGWPLVGSSLSFYQAFKI 68
           A+   +  L ++      +    WY    LG      LPPGDMGWPLVG   +F +AFK 
Sbjct: 8   AVAAVVAALAVVALDAAVRAAHAWYWTASLGAGRRGRLPPGDMGWPLVGGMWAFLRAFK- 67

Query: 69  GGNPNFVMDDLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLL 128
            G P+  +D    RFGR  +Y++ ++  P I+ T PE C+++ +DD+ F   +PK+   L
Sbjct: 68  SGRPDSFIDSFARRFGRAGLYRAFMFSSPTIMATTPEACKQVLMDDDAFVTGWPKATVAL 127

Query: 129 QADGSLLKVDY---KIVHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSM--KGPVPL 188
               S + + Y   + + +L AAPING +AL+ Y+ FI+D VV  L  W      G    
Sbjct: 128 IGPKSFVNMGYDEHRRLRKLTAAPINGFDALTSYLGFIDDTVVTTLRGWSERGGDGHFEF 187

Query: 189 LHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKAR 248
           L E +R+TF+II+ +F+G    +   AELE  Y E+    M     +LPGF+Y +A++AR
Sbjct: 188 LTELRRMTFRIIVQIFMGGA-DERTAAELERTYTELNYG-MRAMAIDLPGFAYHKAIRAR 247

Query: 249 IKVEDIVRPVIQKRKKALENNV--DLEETCQLDAVIKATDKNGERVFNDNAIICMLLGLL 308
            ++   ++ V+ +R+                +D +I   D+ G R+  D+ II +L+  L
Sbjct: 248 RRLVAALQRVLDERRARGGKTAAGAAAPVDMMDRLIAVEDEGGRRL-QDDEIIDVLVMYL 307

Query: 309 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKI 368
            AG+ +S H   W  V + ENP++  KAK EQE I++  PP QKGL  ++ ++M Y +++
Sbjct: 308 NAGHESSGHITMWATVFLQENPEILAKAKAEQEAIMRSIPPGQKGLTLRDFRKMAYLSQV 367

Query: 369 INEVLRLNNIVFTNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRW 428
           ++E LR  NI F +FR AT+DV VNGY IPKGW   +W R VHMD ++Y +P +F+PSRW
Sbjct: 368 VDETLRFVNISFVSFRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPKKFDPSRW 427

Query: 429 DNYTPKPGEYIPFGIGSRFCPGNELAKLEMTVLLHHFLLGYR 463
           +   P+ G ++PFG+G+R CPGN+LAKLE++V LHHFLLGY+
Sbjct: 428 EGPPPRAGTFLPFGLGTRLCPGNDLAKLEISVFLHHFLLGYK 465

[Close]

BLAST of MS005444 vs. ExPASy TrEMBL
Match: A0A5K7U6A4 (Cytochrome P450 monooxygenase OS=Momordica charantia OX=3673 GN=McCYP88L7 PE=2 SV=1)

HSP 1 Score: 929.9 bits (2402), Expect = 4.2e-267
Identity = 451/462 (97.62%), Postives = 458/462 (99.13%), Query Frame = 0


Query: 1   MELLSNFGAILGPILGLYILVFGFMKKFNDIWYRFRLGKNVYNALPPGDMGWPLVGSSLS 60
           MELLSNFGAILG +LGLYILVFGF++KFNDIWYRF+LGKNVYNALPPGDMGWPLVGSSLS
Sbjct: 1   MELLSNFGAILGALLGLYILVFGFLRKFNDIWYRFKLGKNVYNALPPGDMGWPLVGSSLS 60

Query: 61  FYQAFKIGGNPNFVMDDLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNY 120
           FYQAFKIGG+PN VMD+LISRFGRVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNY
Sbjct: 61  FYQAFKIGGDPNAVMDNLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNY 120

Query: 121 PKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPV 180
           PKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPV
Sbjct: 121 PKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPV 180

Query: 181 PLLHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALK 240
           PLLHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALK
Sbjct: 181 PLLHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALK 240

Query: 241 ARIKVEDIVRPVIQKRKKALENNVDLEETCQLDAVIKATDKNGERVFNDNAIICMLLGLL 300
           ARIKVE+IVRPVIQ RKKALENNVDLEETCQLDAVIKATDKNGERVFNDNAIICMLLGLL
Sbjct: 241 ARIKVENIVRPVIQNRKKALENNVDLEETCQLDAVIKATDKNGERVFNDNAIICMLLGLL 300

Query: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKI 360
           YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPP QKGLNFKEIKQMKYTAKI
Sbjct: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPPNQKGLNFKEIKQMKYTAKI 360

Query: 361 INEVLRLNNIVFTNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRW 420
           INEVLRLNNIVFTNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRW
Sbjct: 361 INEVLRLNNIVFTNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRW 420

Query: 421 DNYTPKPGEYIPFGIGSRFCPGNELAKLEMTVLLHHFLLGYR 463
           DNYTPKPGEYIPFGIGSRFCPGNELAKLEMTVLLHHFLLGYR
Sbjct: 421 DNYTPKPGEYIPFGIGSRFCPGNELAKLEMTVLLHHFLLGYR 462

[Close]

BLAST of MS005444 vs. ExPASy TrEMBL
Match: A0A5K7U6M8 (Cytochrome P450 monooxygenase OS=Momordica charantia OX=3673 GN=McCYP88L8 PE=2 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 5.8e-224
Identity = 366/462 (79.22%), Postives = 420/462 (90.91%), Query Frame = 0


Query: 1   MELLSNFGAILGPILGLYILVFGFMKKFNDIWYRFRLGKNVYNALPPGDMGWPLVGSSLS 60
           ME+L+NF A+LG  +GLY  VFGF++ FN++WY  RLG  +YNALPPG MGWPL+GSSLS
Sbjct: 1   MEILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLS 60

Query: 61  FYQAFKIGGNPNFVMDDLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNY 120
           FY+AFKIGG+PN +++ L+SR+GRVRMYKSHLYG PVIIVTDPEVCRR+YLDDE FKL+Y
Sbjct: 61  FYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSY 120

Query: 121 PKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPV 180
           PKSV +LQ D SLLK+DYKIVHRLMAAPING EALS+Y+ FIE+AVV+GLEEWGS+KGPV
Sbjct: 121 PKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPV 180

Query: 181 PLLHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALK 240
            LL EFKRLTFK+I GVFLGP FTDSE+A+LE+LYAEIG AI+SIFPY+LPGFSYRRALK
Sbjct: 181 ELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALK 240

Query: 241 ARIKVEDIVRPVIQKRKKALENNVDLEETCQLDAVIKATDKNGERVFNDNAIICMLLGLL 300
           AR KVE+IVRPVI++R++ LE N +LEETCQ+DAVIKATDKNGE++FNDNAIICMLLGLL
Sbjct: 241 ARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIICMLLGLL 300

Query: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKI 360
           YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRP TQKGLNFKEIKQMKY  K+
Sbjct: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKV 360

Query: 361 INEVLRLNNIVFTNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRW 420
           INE+LRLNNIVFT FR+AT DVNVNGY IPKGW   +W RGVHMD +IY+NPLEF+PSRW
Sbjct: 361 INEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRW 420

Query: 421 DNYTPKPGEYIPFGIGSRFCPGNELAKLEMTVLLHHFLLGYR 463
           DNY PKPGE+IPFG+GSR+CPGNELA+LE+ +LLHHFLLGYR
Sbjct: 421 DNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 462

[Close]

BLAST of MS005444 vs. ExPASy TrEMBL
Match: A0A6J1DVM6 (beta-amyrin 11-oxidase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023928 PE=3 SV=1)

HSP 1 Score: 785.0 bits (2026), Expect = 1.7e-223
Identity = 365/462 (79.00%), Postives = 419/462 (90.69%), Query Frame = 0


Query: 1   MELLSNFGAILGPILGLYILVFGFMKKFNDIWYRFRLGKNVYNALPPGDMGWPLVGSSLS 60
           ME+L+NF A+LG  +GLY  VFGF++ FN++WY  RLG  +YNALPPG MGWPL+GSSLS
Sbjct: 1   MEILNNFWALLGASIGLYFFVFGFLRNFNELWYFLRLGGKIYNALPPGHMGWPLLGSSLS 60

Query: 61  FYQAFKIGGNPNFVMDDLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNY 120
           FY+AFKIGG+PN +++ L+SR+GRVRMYKSHLYG PVIIVTDPEVCRR+YLDDE FKL+Y
Sbjct: 61  FYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRVYLDDERFKLSY 120

Query: 121 PKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPV 180
           PKSV +LQ D SLLK+DYKIVHRLMAAPING EALS+Y+ FIE+AVV+GLEEWGS+KGPV
Sbjct: 121 PKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKGLEEWGSVKGPV 180

Query: 181 PLLHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALK 240
            LL EFKRLTFK+I GVFLGP FTDSE+A+LE+LYAEIG AI+SIFPY+LPGFSYRRALK
Sbjct: 181 ELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYDLPGFSYRRALK 240

Query: 241 ARIKVEDIVRPVIQKRKKALENNVDLEETCQLDAVIKATDKNGERVFNDNAIICMLLGLL 300
           AR KVE+IVRPVI++R++ LE N +LEETCQ+DAVIKATDKNGE++FNDNAI CMLLGLL
Sbjct: 241 ARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFNDNAIFCMLLGLL 300

Query: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKI 360
           YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRP TQKGLNFKEIKQMKY  K+
Sbjct: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFKEIKQMKYATKV 360

Query: 361 INEVLRLNNIVFTNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRW 420
           INE+LRLNNIVFT FR+AT DVNVNGY IPKGW   +W RGVHMD +IY+NPLEF+PSRW
Sbjct: 361 INEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIYQNPLEFDPSRW 420

Query: 421 DNYTPKPGEYIPFGIGSRFCPGNELAKLEMTVLLHHFLLGYR 463
           DNY PKPGE+IPFG+GSR+CPGNELA+LE+ +LLHHFLLGYR
Sbjct: 421 DNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 462

[Close]

BLAST of MS005444 vs. ExPASy TrEMBL
Match: A0A6J1DX36 (beta-amyrin 11-oxidase-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023928 PE=3 SV=1)

HSP 1 Score: 719.2 bits (1855), Expect = 1.1e-203
Identity = 336/413 (81.36%), Postives = 381/413 (92.25%), Query Frame = 0


Query: 50  MGWPLVGSSLSFYQAFKIGGNPNFVMDDLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRI 109
           MGWPL+GSSLSFY+AFKIGG+PN +++ L+SR+GRVRMYKSHLYG PVIIVTDPEVCRR+
Sbjct: 1   MGWPLLGSSLSFYKAFKIGGDPNSIINHLVSRYGRVRMYKSHLYGSPVIIVTDPEVCRRV 60

Query: 110 YLDDEHFKLNYPKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVEFIEDAVVEG 169
           YLDDE FKL+YPKSV +LQ D SLLK+DYKIVHRLMAAPING EALS+Y+ FIE+AVV+G
Sbjct: 61  YLDDERFKLSYPKSVNILQGDRSLLKIDYKIVHRLMAAPINGSEALSKYMGFIEEAVVKG 120

Query: 170 LEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYN 229
           LEEWGS+KGPV LL EFKRLTFK+I GVFLGP FTDSE+A+LE+LYAEIG AI+SIFPY+
Sbjct: 121 LEEWGSVKGPVELLLEFKRLTFKVITGVFLGPAFTDSEIAQLENLYAEIGLAILSIFPYD 180

Query: 230 LPGFSYRRALKARIKVEDIVRPVIQKRKKALENNVDLEETCQLDAVIKATDKNGERVFND 289
           LPGFSYRRALKAR KVE+IVRPVI++R++ LE N +LEETCQ+DAVIKATDKNGE++FND
Sbjct: 181 LPGFSYRRALKARSKVENIVRPVIERRRRILEENEELEETCQMDAVIKATDKNGEKIFND 240

Query: 290 NAIICMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPPTQKGLNFK 349
           NAI CMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRP TQKGLNFK
Sbjct: 241 NAIFCMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPSTQKGLNFK 300

Query: 350 EIKQMKYTAKIINEVLRLNNIVFTNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIY 409
           EIKQMKY  K+INE+LRLNNIVFT FR+AT DVNVNGY IPKGW   +W RGVHMD +IY
Sbjct: 301 EIKQMKYATKVINEMLRLNNIVFTTFRVATTDVNVNGYIIPKGWAVPIWNRGVHMDPEIY 360

Query: 410 ENPLEFNPSRWDNYTPKPGEYIPFGIGSRFCPGNELAKLEMTVLLHHFLLGYR 463
           +NPLEF+PSRWDNY PKPGE+IPFG+GSR+CPGNELA+LE+ +LLHHFLLGYR
Sbjct: 361 QNPLEFDPSRWDNYVPKPGEFIPFGLGSRYCPGNELARLEICILLHHFLLGYR 413

[Close]

BLAST of MS005444 vs. ExPASy TrEMBL
Match: A0A6J1GJM1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454910 PE=3 SV=1)

HSP 1 Score: 571.2 bits (1471), Expect = 3.8e-159
Identity = 264/462 (57.14%), Postives = 350/462 (75.76%), Query Frame = 0


Query: 1   MELLSNFGAILGPILGLYILVFGFMKKFNDIWYRFRLGKNVYNALPPGDMGWPLVGSSLS 60
           ME++ N   I+G +LG+Y+    F++K N+IWY  +LG+  Y +LPPGDMGWPL+GSS S
Sbjct: 1   MEMI-NVCTIIGAVLGVYM----FVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFS 60

Query: 61  FYQAFKIGGNPNFVMDDLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNY 120
           FY AF +GG+P+  +  L+SR GRV MYKSHLYG P IIVTDPE+CRRIYLDDE FK NY
Sbjct: 61  FYNAFTVGGDPDSFIYHLLSRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDEIFKPNY 120

Query: 121 PKSVKLLQADGSLLKVDYKIVHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPV 180
           PKSVK+L+ +G   + D+K  +R+MAAP+NG E LS YV+FI+  + +GLEEW SMK P+
Sbjct: 121 PKSVKILEVNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSMKEPI 180

Query: 181 PLLHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALK 240
            LL E   L FK+I  +FLG     S +AELE+LY ++GPAI+SI PY++PG  Y RALK
Sbjct: 181 ELLGEIGSLFFKVISRIFLGTDIGASRMAELETLYKDLGPAILSILPYDVPGLVYHRALK 240

Query: 241 ARIKVEDIVRPVIQKRKKALENNVDLEETCQLDAVIKATDKNGERVFNDNAIICMLLGLL 300
           AR K+E+++  VI+++++ALE     E   Q+D +I A D+NG +++++N II +LLGLL
Sbjct: 241 ARKKIENVLECVIEEKRRALEQKETREVHSQMDKLIMARDENGMKLYDNNTIIDLLLGLL 300

Query: 301 YAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKI 360
           +AG+HT A+ A W +VH+SE P ++ KAKEEQE I+KQRP TQKGL F+EIKQMKY  K 
Sbjct: 301 HAGHHTPAYAAMWALVHISEKPHIFHKAKEEQESILKQRPSTQKGLTFQEIKQMKYLMKF 360

Query: 361 INEVLRLNNIVFTNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRW 420
           INE+LR N IV  +FR A  D+N+N Y IPKGW   +W R VHMD ++Y NP EF+PSRW
Sbjct: 361 INELLRRNTIVVASFRKAKTDININDYIIPKGWSVLIWTRAVHMDPQLYSNPEEFDPSRW 420

Query: 421 DNYTPKPGEYIPFGIGSRFCPGNELAKLEMTVLLHHFLLGYR 463
           +N++PKPG +IPFGIG+RFCPG+EL KLE+T+LLHHF+L Y+
Sbjct: 421 ENHSPKPGTFIPFGIGTRFCPGSELTKLEITILLHHFILNYK 457

[Close]

BLAST of MS005444 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 376.7 bits (966), Expect = 2.7e-104
Identity = 193/452 (42.70%), Postives = 287/452 (63.50%), Query Frame = 0


Query: 14  ILGLYILVFGFMKKFNDIWYRFRLGKNVYNALPPGDMGWPLVGSSLSFYQAFKIGGNPNF 73
           ILGL++L +  +K+ N   Y  +LG+  +  LPPGD+GWP++G+  SF +AFK   +P  
Sbjct: 15  ILGLFVLKW-VLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFKT-SDPES 74

Query: 74  VMDDLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSL 133
            +   I+R+GR  +YK+H++G+P ++VT PE CRR+  DD+ F + +PKS   L    S 
Sbjct: 75  FIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSF 134

Query: 134 LKV---DYKIVHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLT 193
           + +   ++K + RL +AP+NG EALS Y++FIE+ V   LE+W  M G +  L   ++LT
Sbjct: 135 VGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKM-GEIEFLSHLRKLT 194

Query: 194 FKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVEDIVR 253
           FK+I+ +FL    ++  +  LE  Y  +   + ++   NLPGF+Y RALKAR K+    +
Sbjct: 195 FKVIMYIFLSSE-SEHVMDSLEREYTNLNYGVRAM-GINLPGFAYHRALKARKKLVAAFQ 254

Query: 254 PVIQKRKKALENNVDLEETCQLDAVIKATDKNGERVFNDNAIICMLLGLLYAGYHTSAHG 313
            ++  R+   + N+       LD +I   D+NG RV +D  II +LL  L AG+ +S H 
Sbjct: 255 SIVTNRRNQRKQNISSNRKDMLDNLIDVKDENG-RVLDDEEIIDLLLMYLNAGHESSGHL 314

Query: 314 AQWTMVHVSENPDVYQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKIINEVLRLNNI 373
             W  + + E+P + QKAKEEQE I+K+R P QK L  KE ++M Y +++I+E LR+   
Sbjct: 315 TMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITF 374

Query: 374 VFTNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDNYTPKPGEY 433
             T FR A  DV ++GY IPKGW    W R VH+D +IY +P +F+PSRW+ YTPK G +
Sbjct: 375 SLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTF 434

Query: 434 IPFGIGSRFCPGNELAKLEMTVLLHHFLLGYR 463
           +PFG+GS  CPGN+LAKLE+++ LHHFLL YR
Sbjct: 435 LPFGLGSHLCPGNDLAKLEISIFLHHFLLKYR 458

[Close]

BLAST of MS005444 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 376.7 bits (966), Expect = 2.7e-104
Identity = 193/452 (42.70%), Postives = 287/452 (63.50%), Query Frame = 0


Query: 14  ILGLYILVFGFMKKFNDIWYRFRLGKNVYNALPPGDMGWPLVGSSLSFYQAFKIGGNPNF 73
           ILGL++L +  +K+ N   Y  +LG+  +  LPPGD+GWP++G+  SF +AFK   +P  
Sbjct: 15  ILGLFVLKW-VLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAFKT-SDPES 74

Query: 74  VMDDLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSL 133
            +   I+R+GR  +YK+H++G+P ++VT PE CRR+  DD+ F + +PKS   L    S 
Sbjct: 75  FIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSF 134

Query: 134 LKV---DYKIVHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLT 193
           + +   ++K + RL +AP+NG EALS Y++FIE+ V   LE+W  M G +  L   ++LT
Sbjct: 135 VGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKM-GEIEFLSHLRKLT 194

Query: 194 FKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVEDIVR 253
           FK+I+ +FL    ++  +  LE  Y  +   + ++   NLPGF+Y RALKAR K+    +
Sbjct: 195 FKVIMYIFLSSE-SEHVMDSLEREYTNLNYGVRAM-GINLPGFAYHRALKARKKLVAAFQ 254

Query: 254 PVIQKRKKALENNVDLEETCQLDAVIKATDKNGERVFNDNAIICMLLGLLYAGYHTSAHG 313
            ++  R+   + N+       LD +I   D+NG RV +D  II +LL  L AG+ +S H 
Sbjct: 255 SIVTNRRNQRKQNISSNRKDMLDNLIDVKDENG-RVLDDEEIIDLLLMYLNAGHESSGHL 314

Query: 314 AQWTMVHVSENPDVYQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKIINEVLRLNNI 373
             W  + + E+P + QKAKEEQE I+K+R P QK L  KE ++M Y +++I+E LR+   
Sbjct: 315 TMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITF 374

Query: 374 VFTNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDNYTPKPGEY 433
             T FR A  DV ++GY IPKGW    W R VH+D +IY +P +F+PSRW+ YTPK G +
Sbjct: 375 SLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTF 434

Query: 434 IPFGIGSRFCPGNELAKLEMTVLLHHFLLGYR 463
           +PFG+GS  CPGN+LAKLE+++ LHHFLL YR
Sbjct: 435 LPFGLGSHLCPGNDLAKLEISIFLHHFLLKYR 458

[Close]

BLAST of MS005444 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 349.4 bits (895), Expect = 4.5e-96
Identity = 183/453 (40.40%), Postives = 283/453 (62.47%), Query Frame = 0


Query: 14  ILGLYILVFGFMKKFNDIWYRFRLGKNVYNALPPGDMGWPLVGSSLSFYQAFKIGGNPNF 73
           +LG + L +  ++K N   Y   LG+N  + LPPGD+GWP +G+ LSF +AFK   +P+ 
Sbjct: 16  VLGCFGLNW-LVRKVNVWLYESSLGEN-RHYLPPGDLGWPFIGNMLSFLRAFKT-SDPDS 75

Query: 74  VMDDLISRFGRVRMYKSHLYGFPVIIVTDPEVCRRIYLDDEHFKLNYPKSVKLLQADGSL 133
               LI R+G   +YK+H++G P IIVT  + CRR+  DD+ FK  +P S   L    S 
Sbjct: 76  FTRTLIKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSF 135

Query: 134 LKV---DYKIVHRLMAAPINGVEALSRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLT 193
           + +   ++K + RL AAP+NG EALS Y+ +IE+ V+  L++W  M G    L   ++LT
Sbjct: 136 VGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKM-GEFEFLTHLRKLT 195

Query: 194 FKIIIGVFLGPVFTDSEVAELESLYAEIGPAIMSIFPYNLPGFSYRRALKARIKVEDIVR 253
           F+II+ +FL    +++ +  LE  Y  +   + ++   N+PGF+Y RALKAR  +    +
Sbjct: 196 FRIIMYIFLSSE-SENVMDALEREYTALNYGVRAM-AVNIPGFAYHRALKARKTLVAAFQ 255

Query: 254 PVIQKRKKALENNVDLEETCQLDAVIKATDKNGERVFNDNAIICMLLGLLYAGYHTSAHG 313
            ++ +R+   + N+   +   LD ++   D++G +  +D  II +LL  L AG+ +S H 
Sbjct: 256 SIVTERRNQRKQNILSNKKDMLDNLLNVKDEDG-KTLDDEEIIDVLLMYLNAGHESSGHT 315

Query: 314 AQWTMVHVSENPDVYQKAKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKIINEVLRLNNI 373
             W  V + E+P+V Q+AK EQEMI+K RP  QKGL+ KE ++M++ +++++E LR+   
Sbjct: 316 IMWATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITF 375

Query: 374 VFTNFRMATKDVNVNGYTIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDN-YTPKPGE 433
             T FR A  DV +NGY IPKGW    W R VH+D +++ +P +F+P+RWDN + PK G 
Sbjct: 376 SLTAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGA 435

Query: 434 YIPFGIGSRFCPGNELAKLEMTVLLHHFLLGYR 463
           ++PFG GS  CPGN+LAKLE+++ LHHFLL Y+
Sbjct: 436 FLPFGAGSHLCPGNDLAKLEISIFLHHFLLKYQ 461

[Close]

BLAST of MS005444 vs. TAIR 10
Match: AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )

HSP 1 Score: 216.5 bits (550), Expect = 4.6e-56
Identity = 136/435 (31.26%), Postives = 215/435 (49.43%), Query Frame = 0


Query: 39  KNVYNALPPGDMGWPLVGSSLSFYQAFKIGGNPNFVMDDLISRFGRVRMYKSHLYGFPVI 98
           KN    LPPG MGWP +G +L  Y       NPN        R+G +  +K+ + G+P +
Sbjct: 28  KNSRGKLPPGSMGWPYLGETLQLYSQ-----NPNVFFTSKQKRYGEI--FKTRILGYPCV 87

Query: 99  IVTDPEVCRRIYLDDEH-FKLNYPKSVKLLQADGSLL--KVDYKIVHRLMAAPINGVEAL 158
           ++  PE  R + +   H FK  YP+S + L    +L   + DY    R +       E +
Sbjct: 88  MLASPEAARFVLVTHAHMFKPTYPRSKEKLIGPSALFFHQGDYHSHIRKLVQSSFYPETI 147

Query: 159 SRYVEFIEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKI-IIGVFLGPVFTDSEVAELESL 218
            + +  IE   +  L+ W +M   V    E K+  F + I+ +F            LES 
Sbjct: 148 RKLIPDIEHIALSSLQSWANMP-IVSTYQEMKKFAFDVGILAIF----------GHLESS 207

Query: 219 YAEIGPAIMSI-------FPYNLPGFSYRRALKARIKVEDIVRPVIQKRKKALENNVDLE 278
           Y EI     +I       FP +LPG SY +AL AR +++ IV  +I +R++      D  
Sbjct: 208 YKEILKHNYNIVDKGYNSFPMSLPGTSYHKALMARKQLKTIVSEIICERREKRALQTDF- 267

Query: 279 ETCQLDAVIKATDKNGERVFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQK 338
               L  ++   ++ G RV     I   ++G+L+A   T+A    W + ++ ++  + + 
Sbjct: 268 ----LGHLLNFKNEKG-RVLTQEQIADNIIGVLFAAQDTTASCLTWILKYLHDDQKLLEA 327

Query: 339 AKEEQEMIIKQRPPTQKGLNFKEIKQMKYTAKIINEVLRLNNIVFTNFRMATKDVNVNGY 398
            K EQ+ I ++    +K L +++ + M  T K+I E LR+ +I+   FR A  DV   GY
Sbjct: 328 VKAEQKAIYEENSREKKPLTWRQTRNMPLTHKVIVESLRMASIISFTFREAVVDVEYKGY 387

Query: 399 TIPKGWIAAMWIRGVHMDDKIYENPLEFNPSRWDNYTPKPGEYIPFGIGSRFCPGNELAK 458
            IPKGW      R +H + K + NP  F+PSR++   PKP  ++PFG G   CPGNELAK
Sbjct: 388 LIPKGWKVMPLFRNIHHNPKYFSNPEVFDPSRFE-VNPKPNTFMPFGSGVHACPGNELAK 437

Query: 459 LEMTVLLHHFLLGYR 463
           L++ + LHH +  +R
Sbjct: 448 LQILIFLHHLVSNFR 437

[Close]

BLAST of MS005444 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 206.8 bits (525), Expect = 3.6e-53
Identity = 130/423 (30.73%), Postives = 220/423 (52.01%), Query Frame = 0


Query: 45  LPPGDMGWPLVGSSLSFYQAFKIGGNPNFVMDDLISRFGRVRMYKSHLYGFPVIIVTDPE 104
           LPPG MG P +G +L  Y       NPN       +++G +  +K+H+ G P ++++ PE
Sbjct: 49  LPPGSMGLPYIGETLRLYTE-----NPNSFFATRQNKYGDI--FKTHILGCPCVMISSPE 108

Query: 105 VCRRIYLDDEH-FKLNYPKSVKLLQADGSLL--KVDYKIVHRLMAAPINGVEALSRYVEF 164
             R + +   H FK  YP S + +    +L   +  Y    + +        AL   V  
Sbjct: 109 AARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFMPSALRPTVSH 168

Query: 165 IEDAVVEGLEEWGSMKGPVPLLHEFKRLTFKIIIGVFLGPVFTDSEVAELESLYAEIGPA 224
           IE  V++ L  W S K  +  L   KR  F + I    G     + +  ++ LY  +   
Sbjct: 169 IELLVLQTLSSWTSQKS-INTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERG 228

Query: 225 IMSIFPYNLPGFSYRRALKARIKVEDIVRPVIQKRKKALENNVDLEETCQLDAVIKATD- 284
             S+ P +LPG  + +++KARI++ + +R VI+KR+   EN    EE   L  ++ A D 
Sbjct: 229 YNSM-PLDLPGTLFHKSMKARIELSEELRKVIEKRR---ENG--REEGGLLGVLLGAKDQ 288

Query: 285 -KNGERVFNDNAIICMLLGLLYAGYHTSAHGAQWTMVHVSENPDVYQKAKEEQEMIIKQR 344
            +NG    +D+ I   ++G+++A   T+A    W + ++ ++P++ Q+   EQ  I ++ 
Sbjct: 289 KRNG---LSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKI 348

Query: 345 PPTQKGLNFKEIKQMKYTAKIINEVLRLNNIVFTNFRMATKDVNVNGYTIPKGWIAAMWI 404
               + +++++ ++M  T ++I E LR  +++   FR A +DV  +GY IPKGW      
Sbjct: 349 KKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLF 408

Query: 405 RGVHMDDKIYENPLEFNPSRWDNYTPKPGEYIPFGIGSRFCPGNELAKLEMTVLLHHFLL 463
           R +H   + + +P +F+PSR++   PKP  Y+PFG G   CPG+ELAKLEM +LLHH   
Sbjct: 409 RRIHHSSEFFPDPEKFDPSRFE-VAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTT 453

The following BLAST results are available for this feature:

BLAST of MS005444 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022157153.1	8.7e-267	97.62	beta-amyrin 11-oxidase-like [Momordica charantia] >BBI21929.1 cytochrome P450 mo...	[more]
BBI21930.1	1.2e-223	79.22	cytochrome P450 monooxygenase [Momordica charantia]	[more]
XP_022157134.1	3.5e-223	79.00	beta-amyrin 11-oxidase-like isoform X1 [Momordica charantia]	[more]
XP_022157141.1	2.3e-203	81.36	beta-amyrin 11-oxidase-like isoform X2 [Momordica charantia]	[more]
XP_022952147.1	7.9e-159	57.14	beta-amyrin 11-oxidase-like [Cucurbita moschata]	[more]

BLAST of MS005444 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B5BSX1	2.3e-108	43.61	Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1	[more]
Q9C5Y2	3.7e-103	42.70	Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2	[more]
Q5VRM7	1.2e-101	41.56	Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ...	[more]
Q43246	3.9e-100	41.84	Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1	[more]
Q9AXH9	2.0e-96	39.18	Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1	[more]

BLAST of MS005444 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5K7U6A4	4.2e-267	97.62	Cytochrome P450 monooxygenase OS=Momordica charantia OX=3673 GN=McCYP88L7 PE=2 S...	[more]
A0A5K7U6M8	5.8e-224	79.22	Cytochrome P450 monooxygenase OS=Momordica charantia OX=3673 GN=McCYP88L8 PE=2 S...	[more]
A0A6J1DVM6	1.7e-223	79.00	beta-amyrin 11-oxidase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1DX36	1.1e-203	81.36	beta-amyrin 11-oxidase-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1GJM1	3.8e-159	57.14	beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454910 PE=3 S...	[more]

BLAST of MS005444 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32440.1	2.7e-104	42.70	ent-kaurenoic acid hydroxylase 2	[more]
AT2G32440.2	2.7e-104	42.70	ent-kaurenoic acid hydroxylase 2	[more]
AT1G05160.1	4.5e-96	40.40	cytochrome P450, family 88, subfamily A, polypeptide 3	[more]
AT3G19270.1	4.6e-56	31.26	cytochrome P450, family 707, subfamily A, polypeptide 4	[more]
AT2G29090.1	3.6e-53	30.73	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 398..422 score: 28.02 coord: 440..462 score: 26.81 coord: 430..440 score: 51.16 coord: 311..337 score: 21.5 coord: 358..376 score: 24.82 coord: 291..308 score: 25.6
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 302..319 score: 22.7 coord: 359..370 score: 36.36 coord: 431..440 score: 56.32 coord: 440..451 score: 40.35
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 46..461 e-value: 1.1E-61 score: 209.0
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 39..462 e-value: 8.8E-91 score: 306.9
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 44..461
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 18..460
None	No IPR available	PANTHER	PTHR24286:SF289	ENT-KAURENOIC ACID OXIDASE 2	coord: 18..460
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 433..442

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS005444.1	MS005444.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen