MS005432 (gene) Bitter gourd (TR) v1

BLAST of MS005432 vs. NCBI nr
Match: XP_022157587.1 (tobamovirus multiplication protein 2A [Momordica charantia])

HSP 1 Score: 562.4 bits (1448), Expect = 2.2e-156
Identity = 279/279 (100.00%), Postives = 279/279 (100.00%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYSRSSSDIPSPPLSGDHDLVQLGRP 60
           MACRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYSRSSSDIPSPPLSGDHDLVQLGRP
Sbjct: 1   MACRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYSRSSSDIPSPPLSGDHDLVQLGRP 60

Query: 61  MLMAVSLSSNVFDNLPKAWFIYLFIAVGVILFVVSCFGCIGATTRNGCCLSCYSVLVLLL 120
           MLMAVSLSSNVFDNLPKAWFIYLFIAVGVILFVVSCFGCIGATTRNGCCLSCYSVLVLLL
Sbjct: 61  MLMAVSLSSNVFDNLPKAWFIYLFIAVGVILFVVSCFGCIGATTRNGCCLSCYSVLVLLL 120

Query: 121 ILLQLGGAAFIFFDKHWRDEIPRDKTGNFDKIYEFLDDNWDMIRWVALGAVTFEALLFLF 180
           ILLQLGGAAFIFFDKHWRDEIPRDKTGNFDKIYEFLDDNWDMIRWVALGAVTFEALLFLF
Sbjct: 121 ILLQLGGAAFIFFDKHWRDEIPRDKTGNFDKIYEFLDDNWDMIRWVALGAVTFEALLFLF 180

Query: 181 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSAR 240
           ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSAR
Sbjct: 181 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSAR 240

Query: 241 MREKYGLDTTEFTYNPSESHRFQQVAAQPVEEKSRCTIM 280
           MREKYGLDTTEFTYNPSESHRFQQVAAQPVEEKSRCTIM
Sbjct: 241 MREKYGLDTTEFTYNPSESHRFQQVAAQPVEEKSRCTIM 279

BLAST of MS005432 vs. NCBI nr
Match: XP_022996390.1 (tobamovirus multiplication protein 2A-like [Cucurbita maxima])

HSP 1 Score: 521.2 bits (1341), Expect = 5.7e-144
Identity = 254/279 (91.04%), Postives = 266/279 (95.34%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYSRSSSDIPSPPLSGDHDLVQLGRP 60
           MACRGCFECLLKLLNF LSLLGLAMVGYGIYLLVEY +SSSD+P PPLSGDHD++QLGRP
Sbjct: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSSSDVPRPPLSGDHDVIQLGRP 60

Query: 61  MLMAVSLSSNVFDNLPKAWFIYLFIAVGVILFVVSCFGCIGATTRNGCCLSCYSVLVLLL 120
           MLMAVSLSSN+FDNLP+AWFIYLFIAVG I+FVVSCFGCIGA TRNGCCLSCYSVLVLLL
Sbjct: 61  MLMAVSLSSNIFDNLPRAWFIYLFIAVGAIIFVVSCFGCIGAATRNGCCLSCYSVLVLLL 120

Query: 121 ILLQLGGAAFIFFDKHWRDEIPRDKTGNFDKIYEFLDDNWDMIRWVALGAVTFEALLFLF 180
           IL+QLG AAFIFFDKHWRDEIP DKTGNFDKIYE L+D W++IRWVALGAV FEALLFL 
Sbjct: 121 ILVQLGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEALLFLL 180

Query: 181 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSAR 240
           ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSAR
Sbjct: 181 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSAR 240

Query: 241 MREKYGLDTTEFTYNPSESHRFQQVAAQPVEEKSRCTIM 280
           MREKYGLDT+EFTYNPSESHRFQQVA QP EEKSRCTIM
Sbjct: 241 MREKYGLDTSEFTYNPSESHRFQQVAPQPAEEKSRCTIM 279

BLAST of MS005432 vs. NCBI nr
Match: XP_022931040.1 (tobamovirus multiplication protein 2A-like [Cucurbita moschata] >KAG7036173.1 Tobamovirus multiplication protein 2A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 520.0 bits (1338), Expect = 1.3e-143
Identity = 253/279 (90.68%), Postives = 266/279 (95.34%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYSRSSSDIPSPPLSGDHDLVQLGRP 60
           MACRGCFECLLKLLNF LSLLGLAMVGYGIYLLVEY +SSSD+P PPLSGDHD++QLGRP
Sbjct: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSSSDVPRPPLSGDHDVIQLGRP 60

Query: 61  MLMAVSLSSNVFDNLPKAWFIYLFIAVGVILFVVSCFGCIGATTRNGCCLSCYSVLVLLL 120
           MLMAVSLSSN+FDNLP+AWFIYLFIAVGVI+FVVSCFGCIGA TRNGCCLSCYSVLVLLL
Sbjct: 61  MLMAVSLSSNIFDNLPRAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSVLVLLL 120

Query: 121 ILLQLGGAAFIFFDKHWRDEIPRDKTGNFDKIYEFLDDNWDMIRWVALGAVTFEALLFLF 180
           IL+QLG AAFIFFDKHWRDEIP DKTGNFDKIYE L+D W++IRWVALGAV FEALLFL 
Sbjct: 121 ILVQLGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEALLFLL 180

Query: 181 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSAR 240
           ALVVRAANRP+DYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWS R
Sbjct: 181 ALVVRAANRPLDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSTR 240

Query: 241 MREKYGLDTTEFTYNPSESHRFQQVAAQPVEEKSRCTIM 280
           MREKYGLDT+EFTYNPSESHRFQQVA QP EEKSRCTIM
Sbjct: 241 MREKYGLDTSEFTYNPSESHRFQQVAPQPAEEKSRCTIM 279

BLAST of MS005432 vs. NCBI nr
Match: XP_023533384.1 (tobamovirus multiplication protein 2A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 518.8 bits (1335), Expect = 2.8e-143
Identity = 252/279 (90.32%), Postives = 266/279 (95.34%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYSRSSSDIPSPPLSGDHDLVQLGRP 60
           MACRGCFECLLKLLNF LSLLGLAMVGYGIYLLVEY +SSSD+P PPLSGDHD++QLGRP
Sbjct: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSSSDVPRPPLSGDHDVIQLGRP 60

Query: 61  MLMAVSLSSNVFDNLPKAWFIYLFIAVGVILFVVSCFGCIGATTRNGCCLSCYSVLVLLL 120
           MLMAVSLSSN+FDNLP+AWFIYLFIAVGVI+FVVSCFGCIGA TRNGCCLSCYSVLVLLL
Sbjct: 61  MLMAVSLSSNIFDNLPRAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSVLVLLL 120

Query: 121 ILLQLGGAAFIFFDKHWRDEIPRDKTGNFDKIYEFLDDNWDMIRWVALGAVTFEALLFLF 180
           IL+QLG AAFIFFDKHWRDEIP D+TGNFDKIYE L+D W++IRWVALGAV FEALLFL 
Sbjct: 121 ILVQLGCAAFIFFDKHWRDEIPGDRTGNFDKIYELLEDKWEIIRWVALGAVIFEALLFLL 180

Query: 181 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSAR 240
           ALVVRAANRP+DYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWS R
Sbjct: 181 ALVVRAANRPLDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSTR 240

Query: 241 MREKYGLDTTEFTYNPSESHRFQQVAAQPVEEKSRCTIM 280
           MREKYGLDT+EFTYNPSESHRFQQVA QP EEKSRCTIM
Sbjct: 241 MREKYGLDTSEFTYNPSESHRFQQVAPQPAEEKSRCTIM 279

BLAST of MS005432 vs. NCBI nr
Match: XP_022952814.1 (tobamovirus multiplication protein 2A [Cucurbita moschata])

HSP 1 Score: 515.0 bits (1325), Expect = 4.1e-142
Identity = 253/279 (90.68%), Postives = 263/279 (94.27%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYSRSSSDIPSPPLSGDHDLVQLGRP 60
           MACRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEY +S SDIP PPLSGDHDLVQLGRP
Sbjct: 1   MACRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYLQSPSDIPRPPLSGDHDLVQLGRP 60

Query: 61  MLMAVSLSSNVFDNLPKAWFIYLFIAVGVILFVVSCFGCIGATTRNGCCLSCYSVLVLLL 120
           MLMAVSLSSN+FD+LPKAWFIYLFIAVGVI+FVVSCFGCIGA TRNGCCLSCYSVLVLLL
Sbjct: 61  MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSVLVLLL 120

Query: 121 ILLQLGGAAFIFFDKHWRDEIPRDKTGNFDKIYEFLDDNWDMIRWVALGAVTFEALLFLF 180
           IL+Q+G AAFIFFDKHWRDEIP DKTGNFDKIYE L+D W++IRWVALGAV FEALLFL 
Sbjct: 121 ILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEALLFLL 180

Query: 181 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSAR 240
           ALVVRAAN P DYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAG LDQRPSRNDAWS R
Sbjct: 181 ALVVRAANSPADYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGALDQRPSRNDAWSTR 240

Query: 241 MREKYGLDTTEFTYNPSESHRFQQVAAQPVEEKSRCTIM 280
           MREKYGLDT+EFTYNPSESHRFQQVA QP EEKSRCTIM
Sbjct: 241 MREKYGLDTSEFTYNPSESHRFQQVAQQPAEEKSRCTIM 279

BLAST of MS005432 vs. ExPASy Swiss-Prot
Match: Q9C5W7 (Tobamovirus multiplication protein 2A OS=Arabidopsis thaliana OX=3702 GN=TOM2A PE=1 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 3.2e-118
Identity = 205/280 (73.21%), Postives = 235/280 (83.93%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYSR-SSSDIPSPPLSGDHDLVQLGR 60
           MACRGC ECLLKLLNFLL++ GL M+GYGIYL VEY R + + +     +GD   V  GR
Sbjct: 1   MACRGCLECLLKLLNFLLAVAGLGMIGYGIYLFVEYKRVTDNSVTFDLTNGDQSYVSFGR 60

Query: 61  PMLMAVSLSSNVFDNLPKAWFIYLFIAVGVILFVVSCFGCIGATTRNGCCLSCYSVLVLL 120
           P+LMAVSLSSN+FDNLPKAWFIYLFI +GV LFV+SC GC+G  +R+ CCLSCYS+L++L
Sbjct: 61  PILMAVSLSSNIFDNLPKAWFIYLFIGIGVALFVISCCGCVGTCSRSVCCLSCYSLLLIL 120

Query: 121 LILLQLGGAAFIFFDKHWRDEIPRDKTGNFDKIYEFLDDNWDMIRWVALGAVTFEALLFL 180
           LIL++LG AAFIFFD  WRDE+P D+TGNFD IY FL +NW ++RWVALGAV FEALLFL
Sbjct: 121 LILVELGFAAFIFFDNSWRDELPSDRTGNFDTIYNFLRENWKIVRWVALGAVVFEALLFL 180

Query: 181 FALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSA 240
            AL+VRAAN P +YDSDDEY+APRQQIRQP INR  AP TGVPVA TLDQRPSR+D WSA
Sbjct: 181 LALMVRAANTPAEYDSDDEYLAPRQQIRQPFINRQAAPVTGVPVAPTLDQRPSRSDPWSA 240

Query: 241 RMREKYGLDTTEFTYNPSESHRFQQVAAQPVEEKSRCTIM 280
           RMREKYGLDT+EFTYNPSESHRFQQ+ AQP EEK RCTIM
Sbjct: 241 RMREKYGLDTSEFTYNPSESHRFQQMPAQPNEEKGRCTIM 280

BLAST of MS005432 vs. ExPASy Swiss-Prot
Match: Q93XY5 (Tetraspanin-18 OS=Arabidopsis thaliana OX=3702 GN=TOM2AH2 PE=1 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 3.2e-33
Identity = 93/267 (34.83%), Postives = 141/267 (52.81%), Query Frame = 0

Query: 3   CRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYSRSSSDIPSPPL---SGDHDLVQLGR 62
           C   F   LK+LNF+ + +G++++ Y I++L EYSR     P P     SG      +  
Sbjct: 6   CHVSFASTLKILNFVQAFIGVSIIIYSIWMLHEYSRHLPVDPPPSASSSSGTEIATSVSE 65

Query: 63  PM------LMAVSLSSNVFD--------NLPKAWFIYLFIAVGVILFVVSCFGCIGATTR 122
           P+      + ++ L SN  D        +LP  WFIY F+AVG+++ +V+  G I A   
Sbjct: 66  PLKNPIDFVASIILGSNGGDHGFNLRSLDLPAPWFIYSFMAVGILVCIVTFIGFIAAEAI 125

Query: 123 NGCCLSCYSVLVLLLILLQLGGAAFIFFDKHWRDEIPRDKTGNFDKIYEFLDDNWDMIRW 182
           NGCCL  YS+L  LLILL+    A+I  D+HW  ++P D TG    +  F+++N D+ +W
Sbjct: 126 NGCCLCFYSILKTLLILLEAALVAYIAIDRHWEKDLPYDPTGELSSLRAFIEENIDICKW 185

Query: 183 VALGAVTFEALLFLFALVVRA----ANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGV 242
           V +  V  + L  L A+V+RA        +D + DDE   PR +   PL+     P    
Sbjct: 186 VGIAVVAVQLLSLLLAMVLRAMVSTPKPELDEEEDDE--NPRSRTWDPLL----GPQGNQ 245

Query: 243 PVAGTLDQRPSRNDAWSARMREKYGLD 249
             AG+     S+ + WS+R+REKYGL+
Sbjct: 246 APAGS-----SKIENWSSRIREKYGLN 261

BLAST of MS005432 vs. ExPASy Swiss-Prot
Match: Q9SVU4 (Tetraspanin-20 OS=Arabidopsis thaliana OX=3702 GN=TOM2AH1 PE=1 SV=1)

HSP 1 Score: 136.7 bits (343), Expect = 3.9e-31
Identity = 84/285 (29.47%), Postives = 143/285 (50.18%), Query Frame = 0

Query: 3   CRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYSR-----------SSSDIPSPPLSGD 62
           C   F  +LK+LNFL + +G++++ Y I++L +Y+             ++  P    S  
Sbjct: 6   CHVSFASILKILNFLQAFIGISIIIYSIWMLDQYNHHVPVDPPPSQPPAASSPDSSYSFS 65

Query: 63  HDLVQLGR---------PMLMAVSLSSNVFD--------NLPKAWFIYLFIAVGVILFVV 122
           + ++++             +  + L S   D        +LP  WFIY F+A+G+++ +V
Sbjct: 66  NSIIEINSVSDSLKNPIDFVSGIVLGSGGGDSGFNLRSLDLPAPWFIYCFMAIGILVCIV 125

Query: 123 SCFGCIGATTRNGCCLSCYSVLVLLLILLQLGGAAFIFFDKHWRDEIPRDKTGNFDKIYE 182
           +  G I A   NGCCL  YS+L  LLI+++     FI  D+HW  ++P D TG  + +  
Sbjct: 126 TIIGFIAAEAINGCCLCFYSILKTLLIIIEAALVGFIVIDRHWEKDLPYDPTGELNSLRA 185

Query: 183 FLDDNWDMIRWVALGAVTFEALLFLFALVVRA--ANRPVDYDSDDEYIAPRQQIRQPLIN 242
           F+++N D+ +WV +  V  + L  L ALV+RA  + R  + D +D++  P  + R  L+ 
Sbjct: 186 FIEENIDICKWVGIVVVAIQLLSLLLALVLRAMVSPRQSELDDEDDFENPMSRARDNLLG 245

Query: 243 RPVAPATGVPVAGTLDQRPSRNDAWSARMREKYGLDTTEFTYNPS 258
                    P A       S  D W +R+REKYGL  ++ ++ PS
Sbjct: 246 ---------PQANQTSSGSSNIDNWRSRIREKYGLINSQ-SHTPS 280

BLAST of MS005432 vs. ExPASy Swiss-Prot
Match: Q940P5 (Tetraspanin-19 OS=Arabidopsis thaliana OX=3702 GN=TOM2AH3 PE=2 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.1e-25
Identity = 69/212 (32.55%), Postives = 112/212 (52.83%), Query Frame = 0

Query: 4   RGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYSRSSSDIPSPPLSGDHDLVQLGRPMLM 63
           R C + +LKL+N L+ ++G+AM+ Y ++L+ ++     ++P      DH           
Sbjct: 6   RSCLQSMLKLVNSLIGMVGIAMILYAVWLIRQWQEQMGNLPF--ADSDH----------- 65

Query: 64  AVSLSSNVFDNLPKAWFIYLFIAVGVILFVVSCFGCIGATTRNGCCLSCYSVLVLLLILL 123
                       P  WFIY F+ +G IL VV+C G I A T NGCCL  Y   ++LL ++
Sbjct: 66  ------------PVPWFIYSFLGLGAILCVVTCAGHIAAETVNGCCLYLYMGFIVLLTMV 125

Query: 124 QLGGAAFIFFDKHWRDEIPRDKTGNFDKIYEFLDDNWDMIRWVALGAVTFEALLFLFALV 183
           + G  A IF ++ W+ + P D +G F +  +F++ N+ + +W+ L  V  + L  L A++
Sbjct: 126 EGGVVADIFLNRDWKKDFPEDPSGAFHQFSKFIESNFKICKWIGLSIVCVQGLSVLIAML 185

Query: 184 VRA--ANRPVDYDSDDEY----IAPRQQIRQP 210
           ++A   +    YDSDDEY    +A  Q  RQP
Sbjct: 186 LKALGPHPHRHYDSDDEYNVSTVALLQDARQP 192

BLAST of MS005432 vs. ExPASy Swiss-Prot
Match: Q80WR1 (Tetraspanin-18 OS=Mus musculus OX=10090 GN=Tspan18 PE=2 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 3.1e-04
Identity = 39/165 (23.64%), Postives = 75/165 (45.45%), Query Frame = 0

Query: 3   CRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYSRSSSDIPSPPLSGDHDLVQLGRPML 62
           C  C + L+ + NF + L G  ++G GI++LV+ +     + + PL              
Sbjct: 5   CLSCMKYLMFVFNFFVFLGGACLLGVGIWVLVDPTGFREIVATNPL-------------- 64

Query: 63  MAVSLSSNVFDNLPKAWFIYLFIAVGVILFVVSCFGCIGATTRNGCCLSCYSVLVLLLIL 122
               L++            Y+ +A+G +LF++   GC GA   N C L  + + +L++ L
Sbjct: 65  ----LTTGA----------YIVLAMGGLLFLLGFLGCCGAVRENRCLLLFFFLFILIIFL 124

Query: 123 LQLGGA--AFIFFDKHWRDEIPRDKTGNF--DKIYEFLDDNWDMI 164
           ++L  A  AFIF +   R+   ++ T ++  D   +     W+ +
Sbjct: 125 VELSAAILAFIFREHLTREFFTKELTKHYQGDNDTDVFSATWNSV 141

BLAST of MS005432 vs. ExPASy TrEMBL
Match: A0A6J1DUV7 (tobamovirus multiplication protein 2A OS=Momordica charantia OX=3673 GN=LOC111024239 PE=4 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 1.1e-156
Identity = 279/279 (100.00%), Postives = 279/279 (100.00%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYSRSSSDIPSPPLSGDHDLVQLGRP 60
           MACRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYSRSSSDIPSPPLSGDHDLVQLGRP
Sbjct: 1   MACRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYSRSSSDIPSPPLSGDHDLVQLGRP 60

Query: 61  MLMAVSLSSNVFDNLPKAWFIYLFIAVGVILFVVSCFGCIGATTRNGCCLSCYSVLVLLL 120
           MLMAVSLSSNVFDNLPKAWFIYLFIAVGVILFVVSCFGCIGATTRNGCCLSCYSVLVLLL
Sbjct: 61  MLMAVSLSSNVFDNLPKAWFIYLFIAVGVILFVVSCFGCIGATTRNGCCLSCYSVLVLLL 120

Query: 121 ILLQLGGAAFIFFDKHWRDEIPRDKTGNFDKIYEFLDDNWDMIRWVALGAVTFEALLFLF 180
           ILLQLGGAAFIFFDKHWRDEIPRDKTGNFDKIYEFLDDNWDMIRWVALGAVTFEALLFLF
Sbjct: 121 ILLQLGGAAFIFFDKHWRDEIPRDKTGNFDKIYEFLDDNWDMIRWVALGAVTFEALLFLF 180

Query: 181 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSAR 240
           ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSAR
Sbjct: 181 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSAR 240

Query: 241 MREKYGLDTTEFTYNPSESHRFQQVAAQPVEEKSRCTIM 280
           MREKYGLDTTEFTYNPSESHRFQQVAAQPVEEKSRCTIM
Sbjct: 241 MREKYGLDTTEFTYNPSESHRFQQVAAQPVEEKSRCTIM 279

BLAST of MS005432 vs. ExPASy TrEMBL
Match: A0A6J1K6M6 (tobamovirus multiplication protein 2A-like OS=Cucurbita maxima OX=3661 GN=LOC111491627 PE=4 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 2.7e-144
Identity = 254/279 (91.04%), Postives = 266/279 (95.34%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYSRSSSDIPSPPLSGDHDLVQLGRP 60
           MACRGCFECLLKLLNF LSLLGLAMVGYGIYLLVEY +SSSD+P PPLSGDHD++QLGRP
Sbjct: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSSSDVPRPPLSGDHDVIQLGRP 60

Query: 61  MLMAVSLSSNVFDNLPKAWFIYLFIAVGVILFVVSCFGCIGATTRNGCCLSCYSVLVLLL 120
           MLMAVSLSSN+FDNLP+AWFIYLFIAVG I+FVVSCFGCIGA TRNGCCLSCYSVLVLLL
Sbjct: 61  MLMAVSLSSNIFDNLPRAWFIYLFIAVGAIIFVVSCFGCIGAATRNGCCLSCYSVLVLLL 120

Query: 121 ILLQLGGAAFIFFDKHWRDEIPRDKTGNFDKIYEFLDDNWDMIRWVALGAVTFEALLFLF 180
           IL+QLG AAFIFFDKHWRDEIP DKTGNFDKIYE L+D W++IRWVALGAV FEALLFL 
Sbjct: 121 ILVQLGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEALLFLL 180

Query: 181 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSAR 240
           ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSAR
Sbjct: 181 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSAR 240

Query: 241 MREKYGLDTTEFTYNPSESHRFQQVAAQPVEEKSRCTIM 280
           MREKYGLDT+EFTYNPSESHRFQQVA QP EEKSRCTIM
Sbjct: 241 MREKYGLDTSEFTYNPSESHRFQQVAPQPAEEKSRCTIM 279

BLAST of MS005432 vs. ExPASy TrEMBL
Match: A0A6J1EYE4 (tobamovirus multiplication protein 2A-like OS=Cucurbita moschata OX=3662 GN=LOC111437351 PE=4 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 6.1e-144
Identity = 253/279 (90.68%), Postives = 266/279 (95.34%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYSRSSSDIPSPPLSGDHDLVQLGRP 60
           MACRGCFECLLKLLNF LSLLGLAMVGYGIYLLVEY +SSSD+P PPLSGDHD++QLGRP
Sbjct: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVEYLQSSSDVPRPPLSGDHDVIQLGRP 60

Query: 61  MLMAVSLSSNVFDNLPKAWFIYLFIAVGVILFVVSCFGCIGATTRNGCCLSCYSVLVLLL 120
           MLMAVSLSSN+FDNLP+AWFIYLFIAVGVI+FVVSCFGCIGA TRNGCCLSCYSVLVLLL
Sbjct: 61  MLMAVSLSSNIFDNLPRAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSVLVLLL 120

Query: 121 ILLQLGGAAFIFFDKHWRDEIPRDKTGNFDKIYEFLDDNWDMIRWVALGAVTFEALLFLF 180
           IL+QLG AAFIFFDKHWRDEIP DKTGNFDKIYE L+D W++IRWVALGAV FEALLFL 
Sbjct: 121 ILVQLGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEALLFLL 180

Query: 181 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSAR 240
           ALVVRAANRP+DYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWS R
Sbjct: 181 ALVVRAANRPLDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSTR 240

Query: 241 MREKYGLDTTEFTYNPSESHRFQQVAAQPVEEKSRCTIM 280
           MREKYGLDT+EFTYNPSESHRFQQVA QP EEKSRCTIM
Sbjct: 241 MREKYGLDTSEFTYNPSESHRFQQVAPQPAEEKSRCTIM 279

BLAST of MS005432 vs. ExPASy TrEMBL
Match: A0A6J1GMT3 (tobamovirus multiplication protein 2A OS=Cucurbita moschata OX=3662 GN=LOC111455402 PE=4 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 2.0e-142
Identity = 253/279 (90.68%), Postives = 263/279 (94.27%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYSRSSSDIPSPPLSGDHDLVQLGRP 60
           MACRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEY +S SDIP PPLSGDHDLVQLGRP
Sbjct: 1   MACRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYLQSPSDIPRPPLSGDHDLVQLGRP 60

Query: 61  MLMAVSLSSNVFDNLPKAWFIYLFIAVGVILFVVSCFGCIGATTRNGCCLSCYSVLVLLL 120
           MLMAVSLSSN+FD+LPKAWFIYLFIAVGVI+FVVSCFGCIGA TRNGCCLSCYSVLVLLL
Sbjct: 61  MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSVLVLLL 120

Query: 121 ILLQLGGAAFIFFDKHWRDEIPRDKTGNFDKIYEFLDDNWDMIRWVALGAVTFEALLFLF 180
           IL+Q+G AAFIFFDKHWRDEIP DKTGNFDKIYE L+D W++IRWVALGAV FEALLFL 
Sbjct: 121 ILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIRWVALGAVIFEALLFLL 180

Query: 181 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSAR 240
           ALVVRAAN P DYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAG LDQRPSRNDAWS R
Sbjct: 181 ALVVRAANSPADYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGALDQRPSRNDAWSTR 240

Query: 241 MREKYGLDTTEFTYNPSESHRFQQVAAQPVEEKSRCTIM 280
           MREKYGLDT+EFTYNPSESHRFQQVA QP EEKSRCTIM
Sbjct: 241 MREKYGLDTSEFTYNPSESHRFQQVAQQPAEEKSRCTIM 279

BLAST of MS005432 vs. ExPASy TrEMBL
Match: A0A6J1I3F8 (tobamovirus multiplication protein 2A-like OS=Cucurbita maxima OX=3661 GN=LOC111470643 PE=4 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 1.8e-140
Identity = 249/279 (89.25%), Postives = 261/279 (93.55%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYSRSSSDIPSPPLSGDHDLVQLGRP 60
           MACRGCFECLLKLLNF LSLLGLAMVGYGIYLLV+Y +S SDIP PPLSGDHDLVQLGRP
Sbjct: 1   MACRGCFECLLKLLNFFLSLLGLAMVGYGIYLLVDYLQSPSDIPRPPLSGDHDLVQLGRP 60

Query: 61  MLMAVSLSSNVFDNLPKAWFIYLFIAVGVILFVVSCFGCIGATTRNGCCLSCYSVLVLLL 120
           MLMAVSLSSN+FD+LPKAWFIYLFIAVGVI+FVVSCFGCIGA TRNGCCLSCYSVLVLLL
Sbjct: 61  MLMAVSLSSNIFDHLPKAWFIYLFIAVGVIIFVVSCFGCIGAATRNGCCLSCYSVLVLLL 120

Query: 121 ILLQLGGAAFIFFDKHWRDEIPRDKTGNFDKIYEFLDDNWDMIRWVALGAVTFEALLFLF 180
           IL+Q+G AAFIFFDKHWRDEIP DKTGNFDKIYE L+D W++I+WVALGAV FEALLFL 
Sbjct: 121 ILVQVGCAAFIFFDKHWRDEIPGDKTGNFDKIYELLEDKWEIIKWVALGAVIFEALLFLL 180

Query: 181 ALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSAR 240
           ALVVRAAN P DYDSDDEYIAPR QIRQPLINRPVAPATGVPVAG LDQRPSRNDAWS R
Sbjct: 181 ALVVRAANSPADYDSDDEYIAPRLQIRQPLINRPVAPATGVPVAGALDQRPSRNDAWSTR 240

Query: 241 MREKYGLDTTEFTYNPSESHRFQQVAAQPVEEKSRCTIM 280
           MREKYGLDT+EFTYNPSESHRFQQVA QP EEKSRCTIM
Sbjct: 241 MREKYGLDTSEFTYNPSESHRFQQVAQQPAEEKSRCTIM 279

BLAST of MS005432 vs. TAIR 10
Match: AT1G32400.1 (tobamovirus multiplication 2A )

HSP 1 Score: 426.0 bits (1094), Expect = 2.3e-119
Identity = 205/280 (73.21%), Postives = 235/280 (83.93%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYSR-SSSDIPSPPLSGDHDLVQLGR 60
           MACRGC ECLLKLLNFLL++ GL M+GYGIYL VEY R + + +     +GD   V  GR
Sbjct: 1   MACRGCLECLLKLLNFLLAVAGLGMIGYGIYLFVEYKRVTDNSVTFDLTNGDQSYVSFGR 60

Query: 61  PMLMAVSLSSNVFDNLPKAWFIYLFIAVGVILFVVSCFGCIGATTRNGCCLSCYSVLVLL 120
           P+LMAVSLSSN+FDNLPKAWFIYLFI +GV LFV+SC GC+G  +R+ CCLSCYS+L++L
Sbjct: 61  PILMAVSLSSNIFDNLPKAWFIYLFIGIGVALFVISCCGCVGTCSRSVCCLSCYSLLLIL 120

Query: 121 LILLQLGGAAFIFFDKHWRDEIPRDKTGNFDKIYEFLDDNWDMIRWVALGAVTFEALLFL 180
           LIL++LG AAFIFFD  WRDE+P D+TGNFD IY FL +NW ++RWVALGAV FEALLFL
Sbjct: 121 LILVELGFAAFIFFDNSWRDELPSDRTGNFDTIYNFLRENWKIVRWVALGAVVFEALLFL 180

Query: 181 FALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSA 240
            AL+VRAAN P +YDSDDEY+APRQQIRQP INR  AP TGVPVA TLDQRPSR+D WSA
Sbjct: 181 LALMVRAANTPAEYDSDDEYLAPRQQIRQPFINRQAAPVTGVPVAPTLDQRPSRSDPWSA 240

Query: 241 RMREKYGLDTTEFTYNPSESHRFQQVAAQPVEEKSRCTIM 280
           RMREKYGLDT+EFTYNPSESHRFQQ+ AQP EEK RCTIM
Sbjct: 241 RMREKYGLDTSEFTYNPSESHRFQQMPAQPNEEKGRCTIM 280

BLAST of MS005432 vs. TAIR 10
Match: AT1G32400.2 (tobamovirus multiplication 2A )

HSP 1 Score: 426.0 bits (1094), Expect = 2.3e-119
Identity = 205/280 (73.21%), Postives = 235/280 (83.93%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYSR-SSSDIPSPPLSGDHDLVQLGR 60
           MACRGC ECLLKLLNFLL++ GL M+GYGIYL VEY R + + +     +GD   V  GR
Sbjct: 1   MACRGCLECLLKLLNFLLAVAGLGMIGYGIYLFVEYKRVTDNSVTFDLTNGDQSYVSFGR 60

Query: 61  PMLMAVSLSSNVFDNLPKAWFIYLFIAVGVILFVVSCFGCIGATTRNGCCLSCYSVLVLL 120
           P+LMAVSLSSN+FDNLPKAWFIYLFI +GV LFV+SC GC+G  +R+ CCLSCYS+L++L
Sbjct: 61  PILMAVSLSSNIFDNLPKAWFIYLFIGIGVALFVISCCGCVGTCSRSVCCLSCYSLLLIL 120

Query: 121 LILLQLGGAAFIFFDKHWRDEIPRDKTGNFDKIYEFLDDNWDMIRWVALGAVTFEALLFL 180
           LIL++LG AAFIFFD  WRDE+P D+TGNFD IY FL +NW ++RWVALGAV FEALLFL
Sbjct: 121 LILVELGFAAFIFFDNSWRDELPSDRTGNFDTIYNFLRENWKIVRWVALGAVVFEALLFL 180

Query: 181 FALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSA 240
            AL+VRAAN P +YDSDDEY+APRQQIRQP INR  AP TGVPVA TLDQRPSR+D WSA
Sbjct: 181 LALMVRAANTPAEYDSDDEYLAPRQQIRQPFINRQAAPVTGVPVAPTLDQRPSRSDPWSA 240

Query: 241 RMREKYGLDTTEFTYNPSESHRFQQVAAQPVEEKSRCTIM 280
           RMREKYGLDT+EFTYNPSESHRFQQ+ AQP EEK RCTIM
Sbjct: 241 RMREKYGLDTSEFTYNPSESHRFQQMPAQPNEEKGRCTIM 280

BLAST of MS005432 vs. TAIR 10
Match: AT1G32400.3 (tobamovirus multiplication 2A )

HSP 1 Score: 426.0 bits (1094), Expect = 2.3e-119
Identity = 205/280 (73.21%), Postives = 235/280 (83.93%), Query Frame = 0

Query: 1   MACRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYSR-SSSDIPSPPLSGDHDLVQLGR 60
           MACRGC ECLLKLLNFLL++ GL M+GYGIYL VEY R + + +     +GD   V  GR
Sbjct: 1   MACRGCLECLLKLLNFLLAVAGLGMIGYGIYLFVEYKRVTDNSVTFDLTNGDQSYVSFGR 60

Query: 61  PMLMAVSLSSNVFDNLPKAWFIYLFIAVGVILFVVSCFGCIGATTRNGCCLSCYSVLVLL 120
           P+LMAVSLSSN+FDNLPKAWFIYLFI +GV LFV+SC GC+G  +R+ CCLSCYS+L++L
Sbjct: 61  PILMAVSLSSNIFDNLPKAWFIYLFIGIGVALFVISCCGCVGTCSRSVCCLSCYSLLLIL 120

Query: 121 LILLQLGGAAFIFFDKHWRDEIPRDKTGNFDKIYEFLDDNWDMIRWVALGAVTFEALLFL 180
           LIL++LG AAFIFFD  WRDE+P D+TGNFD IY FL +NW ++RWVALGAV FEALLFL
Sbjct: 121 LILVELGFAAFIFFDNSWRDELPSDRTGNFDTIYNFLRENWKIVRWVALGAVVFEALLFL 180

Query: 181 FALVVRAANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGVPVAGTLDQRPSRNDAWSA 240
            AL+VRAAN P +YDSDDEY+APRQQIRQP INR  AP TGVPVA TLDQRPSR+D WSA
Sbjct: 181 LALMVRAANTPAEYDSDDEYLAPRQQIRQPFINRQAAPVTGVPVAPTLDQRPSRSDPWSA 240

Query: 241 RMREKYGLDTTEFTYNPSESHRFQQVAAQPVEEKSRCTIM 280
           RMREKYGLDT+EFTYNPSESHRFQQ+ AQP EEK RCTIM
Sbjct: 241 RMREKYGLDTSEFTYNPSESHRFQQMPAQPNEEKGRCTIM 280

BLAST of MS005432 vs. TAIR 10
Match: AT2G20230.1 (Tetraspanin family protein )

HSP 1 Score: 143.7 bits (361), Expect = 2.3e-34
Identity = 93/267 (34.83%), Postives = 141/267 (52.81%), Query Frame = 0

Query: 3   CRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYSRSSSDIPSPPL---SGDHDLVQLGR 62
           C   F   LK+LNF+ + +G++++ Y I++L EYSR     P P     SG      +  
Sbjct: 6   CHVSFASTLKILNFVQAFIGVSIIIYSIWMLHEYSRHLPVDPPPSASSSSGTEIATSVSE 65

Query: 63  PM------LMAVSLSSNVFD--------NLPKAWFIYLFIAVGVILFVVSCFGCIGATTR 122
           P+      + ++ L SN  D        +LP  WFIY F+AVG+++ +V+  G I A   
Sbjct: 66  PLKNPIDFVASIILGSNGGDHGFNLRSLDLPAPWFIYSFMAVGILVCIVTFIGFIAAEAI 125

Query: 123 NGCCLSCYSVLVLLLILLQLGGAAFIFFDKHWRDEIPRDKTGNFDKIYEFLDDNWDMIRW 182
           NGCCL  YS+L  LLILL+    A+I  D+HW  ++P D TG    +  F+++N D+ +W
Sbjct: 126 NGCCLCFYSILKTLLILLEAALVAYIAIDRHWEKDLPYDPTGELSSLRAFIEENIDICKW 185

Query: 183 VALGAVTFEALLFLFALVVRA----ANRPVDYDSDDEYIAPRQQIRQPLINRPVAPATGV 242
           V +  V  + L  L A+V+RA        +D + DDE   PR +   PL+     P    
Sbjct: 186 VGIAVVAVQLLSLLLAMVLRAMVSTPKPELDEEEDDE--NPRSRTWDPLL----GPQGNQ 245

Query: 243 PVAGTLDQRPSRNDAWSARMREKYGLD 249
             AG+     S+ + WS+R+REKYGL+
Sbjct: 246 APAGS-----SKIENWSSRIREKYGLN 261

BLAST of MS005432 vs. TAIR 10
Match: AT4G28770.1 (Tetraspanin family protein )

HSP 1 Score: 136.7 bits (343), Expect = 2.8e-32
Identity = 84/285 (29.47%), Postives = 143/285 (50.18%), Query Frame = 0

Query: 3   CRGCFECLLKLLNFLLSLLGLAMVGYGIYLLVEYSR-----------SSSDIPSPPLSGD 62
           C   F  +LK+LNFL + +G++++ Y I++L +Y+             ++  P    S  
Sbjct: 6   CHVSFASILKILNFLQAFIGISIIIYSIWMLDQYNHHVPVDPPPSQPPAASSPDSSYSFS 65

Query: 63  HDLVQLGR---------PMLMAVSLSSNVFD--------NLPKAWFIYLFIAVGVILFVV 122
           + ++++             +  + L S   D        +LP  WFIY F+A+G+++ +V
Sbjct: 66  NSIIEINSVSDSLKNPIDFVSGIVLGSGGGDSGFNLRSLDLPAPWFIYCFMAIGILVCIV 125

Query: 123 SCFGCIGATTRNGCCLSCYSVLVLLLILLQLGGAAFIFFDKHWRDEIPRDKTGNFDKIYE 182
           +  G I A   NGCCL  YS+L  LLI+++     FI  D+HW  ++P D TG  + +  
Sbjct: 126 TIIGFIAAEAINGCCLCFYSILKTLLIIIEAALVGFIVIDRHWEKDLPYDPTGELNSLRA 185

Query: 183 FLDDNWDMIRWVALGAVTFEALLFLFALVVRA--ANRPVDYDSDDEYIAPRQQIRQPLIN 242
           F+++N D+ +WV +  V  + L  L ALV+RA  + R  + D +D++  P  + R  L+ 
Sbjct: 186 FIEENIDICKWVGIVVVAIQLLSLLLALVLRAMVSPRQSELDDEDDFENPMSRARDNLLG 245

Query: 243 RPVAPATGVPVAGTLDQRPSRNDAWSARMREKYGLDTTEFTYNPS 258
                    P A       S  D W +R+REKYGL  ++ ++ PS
Sbjct: 246 ---------PQANQTSSGSSNIDNWRSRIREKYGLINSQ-SHTPS 280