MS005289 (gene) Bitter gourd (TR) v1

Overview


Name	MS005289
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	PSII 6.1 kDa protein
Location	scaffold83: 605489 .. 605800 (+)
RNA-Seq Expression	MS005289
Synteny	MS005289

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGCTTATGGTGCCAAAGATGAAGAAGGGAGGGATTAAGTGCAAGGTTGAAGAGAGGCCAGAATGTAAGAGCTCAAGAGAAAGCACGGTCTCGGTGACCATGGCGGCGGTGGTGGCAGCTGTGGTGGCAGCAGCGGGTCCAGCGGCGGCGCTGGTGGATGAGAGGTTGAGCACAGAAGGGACAGGGCTGCCCTTTGGCTTAAGCAACAACCTTCTAGCGTGGATTCTTGCAGGGGTGTTTGCTTTGATTTGGGCTCTTTATTTTGTCTATGCTTCATCCTTGGATGAGGATGAAGATTCTGGTTTGTCACTC

mRNA sequence

TTGCTTATGGTGCCAAAGATGAAGAAGGGAGGGATTAAGTGCAAGGTTGAAGAGAGGCCAGAATGTAAGAGCTCAAGAGAAAGCACGGTCTCGGTGACCATGGCGGCGGTGGTGGCAGCTGTGGTGGCAGCAGCGGGTCCAGCGGCGGCGCTGGTGGATGAGAGGTTGAGCACAGAAGGGACAGGGCTGCCCTTTGGCTTAAGCAACAACCTTCTAGCGTGGATTCTTGCAGGGGTGTTTGCTTTGATTTGGGCTCTTTATTTTGTCTATGCTTCATCCTTGGATGAGGATGAAGATTCTGGTTTGTCACTC

Coding sequence (CDS)

TTGCTTATGGTGCCAAAGATGAAGAAGGGAGGGATTAAGTGCAAGGTTGAAGAGAGGCCAGAATGTAAGAGCTCAAGAGAAAGCACGGTCTCGGTGACCATGGCGGCGGTGGTGGCAGCTGTGGTGGCAGCAGCGGGTCCAGCGGCGGCGCTGGTGGATGAGAGGTTGAGCACAGAAGGGACAGGGCTGCCCTTTGGCTTAAGCAACAACCTTCTAGCGTGGATTCTTGCAGGGGTGTTTGCTTTGATTTGGGCTCTTTATTTTGTCTATGCTTCATCCTTGGATGAGGATGAAGATTCTGGTTTGTCACTC

Protein sequence

LLMVPKMKKGGIKCKVEERPECKSSRESTVSVTMAAVVAAVVAAAGPAAALVDERLSTEGTGLPFGLSNNLLAWILAGVFALIWALYFVYASSLDEDEDSGLSL

Homology

[Close]

BLAST of MS005289 vs. NCBI nr
Match: XP_022143021.1 (photosystem II reaction center W protein, chloroplastic-like [Momordica charantia])

HSP 1 Score: 192.6 bits (488), Expect = 1.7e-45
Identity = 103/104 (99.04%), Postives = 103/104 (99.04%), Query Frame = 0


Query: 1   LLMVPKMKKGGIKCKVEERPECKSSRESTVSVTMAAVVAAVVAAAGPAAALVDERLSTEG 60
           LLMVPKMKKGGIKCKVEERPECKSS ESTVSVTMAAVVAAVVAAAGPAAALVDERLSTEG
Sbjct: 30  LLMVPKMKKGGIKCKVEERPECKSSGESTVSVTMAAVVAAVVAAAGPAAALVDERLSTEG 89

Query: 61  TGLPFGLSNNLLAWILAGVFALIWALYFVYASSLDEDEDSGLSL 105
           TGLPFGLSNNLLAWILAGVFALIWALYFVYASSLDEDEDSGLSL
Sbjct: 90  TGLPFGLSNNLLAWILAGVFALIWALYFVYASSLDEDEDSGLSL 133

[Close]

BLAST of MS005289 vs. NCBI nr
Match: XP_038890119.1 (photosystem II reaction center W protein, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 163.7 bits (413), Expect = 8.5e-37
Identity = 88/104 (84.62%), Postives = 92/104 (88.46%), Query Frame = 0


Query: 1   LLMVPKMKKGGIKCKVEERPECKSSRESTVSVTMAAVVAAVVAAAGPAAALVDERLSTEG 60
           L M+ KMKKGGIKC+VEE+PE K S ES VS TMAAV  A +AAAGPA ALVDERLSTEG
Sbjct: 30  LPMLQKMKKGGIKCRVEEKPEGKKSEESLVSGTMAAVAMAALAAAGPAVALVDERLSTEG 89

Query: 61  TGLPFGLSNNLLAWILAGVFALIWALYFVYASSLDEDEDSGLSL 105
           TGLPFGLSNNLL WILAGVFALIWALYFVYASSLDEDEDSGLSL
Sbjct: 90  TGLPFGLSNNLLGWILAGVFALIWALYFVYASSLDEDEDSGLSL 133

[Close]

BLAST of MS005289 vs. NCBI nr
Match: XP_022966002.1 (photosystem II reaction center W protein, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 152.1 bits (383), Expect = 2.6e-33
Identity = 80/104 (76.92%), Postives = 89/104 (85.58%), Query Frame = 0


Query: 1   LLMVPKMKKGGIKCKVEERPECKSSRESTVSVTMAAVVAAVVAAAGPAAALVDERLSTEG 60
           L +V K KKGGIKC++EE+ E KSS+ES +S T+AAV   VVA AGPA ALVDERLSTEG
Sbjct: 30  LSVVQKRKKGGIKCRLEEKQEVKSSKESMISGTVAAVATTVVAMAGPAVALVDERLSTEG 89

Query: 61  TGLPFGLSNNLLAWILAGVFALIWALYFVYASSLDEDEDSGLSL 105
           TGLPFGLSNNLL WILAG+F  IWALYFVYASSL+EDEDSGLSL
Sbjct: 90  TGLPFGLSNNLLGWILAGMFGFIWALYFVYASSLEEDEDSGLSL 133

[Close]

BLAST of MS005289 vs. NCBI nr
Match: XP_023521133.1 (photosystem II reaction center W protein, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_023537055.1 photosystem II reaction center W protein, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 151.8 bits (382), Expect = 3.4e-33
Identity = 80/104 (76.92%), Postives = 89/104 (85.58%), Query Frame = 0


Query: 1   LLMVPKMKKGGIKCKVEERPECKSSRESTVSVTMAAVVAAVVAAAGPAAALVDERLSTEG 60
           L +V KMKKGGIKC++EE+ E K S+ES +S T+AAV   VVA AGPA ALVDERLSTEG
Sbjct: 30  LSVVQKMKKGGIKCRLEEKQEGKRSKESMISGTVAAVATTVVAMAGPAVALVDERLSTEG 89

Query: 61  TGLPFGLSNNLLAWILAGVFALIWALYFVYASSLDEDEDSGLSL 105
           TGLPFGLSNNLL WILAG+F  IWALYFVYASSL+EDEDSGLSL
Sbjct: 90  TGLPFGLSNNLLGWILAGMFGFIWALYFVYASSLEEDEDSGLSL 133

[Close]

BLAST of MS005289 vs. NCBI nr
Match: XP_022937622.1 (photosystem II reaction center W protein, chloroplastic-like [Cucurbita moschata] >KAG6586311.1 Photosystem II reaction center W protein, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021160.1 Photosystem II reaction center W protein, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 151.4 bits (381), Expect = 4.4e-33
Identity = 80/104 (76.92%), Postives = 89/104 (85.58%), Query Frame = 0


Query: 1   LLMVPKMKKGGIKCKVEERPECKSSRESTVSVTMAAVVAAVVAAAGPAAALVDERLSTEG 60
           L +V KMKKGGIKCK+EE+ E K S+ES +S T+AA+   VVA AGPA ALVDERLSTEG
Sbjct: 30  LPVVQKMKKGGIKCKLEEKQEGKRSKESLISGTVAAMATTVVAMAGPAVALVDERLSTEG 89

Query: 61  TGLPFGLSNNLLAWILAGVFALIWALYFVYASSLDEDEDSGLSL 105
           TGLPFGLSNNLL WILAG+F  IWALYFVYASSL+EDEDSGLSL
Sbjct: 90  TGLPFGLSNNLLGWILAGMFGFIWALYFVYASSLEEDEDSGLSL 133

[Close]

BLAST of MS005289 vs. ExPASy Swiss-Prot
Match: Q41387 (Photosystem II reaction center W protein, chloroplastic OS=Spinacia oleracea OX=3562 GN=psbW PE=1 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 2.3e-24
Identity = 63/107 (58.88%), Postives = 78/107 (72.90%), Query Frame = 0


Query: 1   LLMVPKM-KKGGIKCKVEERPECKSSRESTVSVTMAAVVAAVVAA--AGPAAALVDERLS 60
           +L +P M K+  + C +E +P    +  +T     A+++AA  AA  + PA ALVDER+S
Sbjct: 31  ILGLPSMTKRSKVTCSIENKPSTTETTTTTNKSMGASLLAAAAAATISNPAMALVDERMS 90

Query: 61  TEGTGLPFGLSNNLLAWILAGVFALIWALYFVYASSLDEDEDSGLSL 105
           TEGTGLPFGLSNNLL WIL GVF LIWALYFVYAS L+EDE+SGLSL
Sbjct: 91  TEGTGLPFGLSNNLLGWILFGVFGLIWALYFVYASGLEEDEESGLSL 137

[Close]

BLAST of MS005289 vs. ExPASy Swiss-Prot
Match: Q39194 (Photosystem II reaction center W protein, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PSBW PE=1 SV=2)

HSP 1 Score: 109.8 bits (273), Expect = 1.9e-23
Identity = 65/111 (58.56%), Postives = 78/111 (70.27%), Query Frame = 0


Query: 1   LLMVPKM--KKGGIKCKVEERPECKSSRESTVSVTMAAVVAAVVAA-----AGPAAALVD 60
           +L +P M  KKGG++C +E       +++  VSV  A V AA  AA     + PA ALVD
Sbjct: 30  VLGLPPMGKKKGGVRCSME-------TKQGNVSVMGAGVSAAATAALTAVMSNPAMALVD 89

Query: 61  ERLSTEGTGLPFGLSNNLLAWILAGVFALIWALYFVYASSLDEDEDSGLSL 105
           ER+STEGTGLPFGLSNNLL WIL GVF LIW  +FVY SSL+EDE+SGLSL
Sbjct: 90  ERMSTEGTGLPFGLSNNLLGWILFGVFGLIWTFFFVYTSSLEEDEESGLSL 133

[Close]

BLAST of MS005289 vs. ExPASy Swiss-Prot
Match: Q5ZBY9 (Photosystem II reaction center W protein, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=PSBW PE=2 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 4.1e-18
Identity = 50/94 (53.19%), Postives = 62/94 (65.96%), Query Frame = 0


Query: 12  IKCKVEERPECKSSRESTVSVT----MAAVVAAVVAAAGPAAALVDERLSTEGTGLPFGL 71
           ++ +  ER  C  S+E+           A + A+ A A PA ALVDER+STEGTGL  GL
Sbjct: 27  MRARRSERVRCNYSKEAATPAAAVKGAGASLLAMAATAAPAMALVDERMSTEGTGLSLGL 86

Query: 72  SNNLLAWILAGVFALIWALYFVYASSLDEDEDSG 102
           SNNLL WIL GVF LIW+LY +Y S L+EDE+SG
Sbjct: 87  SNNLLGWILLGVFGLIWSLYTIYTSDLEEDEESG 120

[Close]

BLAST of MS005289 vs. ExPASy Swiss-Prot
Match: Q9SPI9 (Photosystem II reaction center W protein, chloroplastic OS=Chlamydomonas reinhardtii OX=3055 GN=psbW PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 1.6e-09
Identity = 43/106 (40.57%), Postives = 61/106 (57.55%), Query Frame = 0


Query: 15  KVEERPECKSSRESTVSV-----TMAAVVAAVVA---------AAGPAAALVDERLSTEG 74
           +V  +P  +S   S V+V     T+ + VA  VA         AA PA ALVDER++ +G
Sbjct: 10  RVAAKPVSRSGARSAVTVVCKATTVRSEVAKKVAMLSTLPATLAAHPAFALVDERMNGDG 69

Query: 75  TGLPFGLSNNLLAWILAGVFALIWALYFVYASSLD--EDEDSGLSL 105
           TG PFG+++ +L W+L GVF  +WA++F+    L   ED D GL L
Sbjct: 70  TGRPFGVNDPVLGWVLLGVFGTMWAIWFIGQKDLGDFEDADDGLKL 115

[Close]

BLAST of MS005289 vs. ExPASy TrEMBL
Match: A0A6J1CNY5 (PSII 6.1 kDa protein OS=Momordica charantia OX=3673 GN=LOC111013009 PE=3 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 8.3e-46
Identity = 103/104 (99.04%), Postives = 103/104 (99.04%), Query Frame = 0


Query: 1   LLMVPKMKKGGIKCKVEERPECKSSRESTVSVTMAAVVAAVVAAAGPAAALVDERLSTEG 60
           LLMVPKMKKGGIKCKVEERPECKSS ESTVSVTMAAVVAAVVAAAGPAAALVDERLSTEG
Sbjct: 30  LLMVPKMKKGGIKCKVEERPECKSSGESTVSVTMAAVVAAVVAAAGPAAALVDERLSTEG 89

Query: 61  TGLPFGLSNNLLAWILAGVFALIWALYFVYASSLDEDEDSGLSL 105
           TGLPFGLSNNLLAWILAGVFALIWALYFVYASSLDEDEDSGLSL
Sbjct: 90  TGLPFGLSNNLLAWILAGVFALIWALYFVYASSLDEDEDSGLSL 133

[Close]

BLAST of MS005289 vs. ExPASy TrEMBL
Match: A0A6J1HLU7 (PSII 6.1 kDa protein OS=Cucurbita maxima OX=3661 GN=LOC111465720 PE=3 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.2e-33
Identity = 80/104 (76.92%), Postives = 89/104 (85.58%), Query Frame = 0


Query: 1   LLMVPKMKKGGIKCKVEERPECKSSRESTVSVTMAAVVAAVVAAAGPAAALVDERLSTEG 60
           L +V K KKGGIKC++EE+ E KSS+ES +S T+AAV   VVA AGPA ALVDERLSTEG
Sbjct: 30  LSVVQKRKKGGIKCRLEEKQEVKSSKESMISGTVAAVATTVVAMAGPAVALVDERLSTEG 89

Query: 61  TGLPFGLSNNLLAWILAGVFALIWALYFVYASSLDEDEDSGLSL 105
           TGLPFGLSNNLL WILAG+F  IWALYFVYASSL+EDEDSGLSL
Sbjct: 90  TGLPFGLSNNLLGWILAGMFGFIWALYFVYASSLEEDEDSGLSL 133

[Close]

BLAST of MS005289 vs. ExPASy TrEMBL
Match: A0A6J1FBR0 (PSII 6.1 kDa protein OS=Cucurbita moschata OX=3662 GN=LOC111443974 PE=3 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 2.1e-33
Identity = 80/104 (76.92%), Postives = 89/104 (85.58%), Query Frame = 0


Query: 1   LLMVPKMKKGGIKCKVEERPECKSSRESTVSVTMAAVVAAVVAAAGPAAALVDERLSTEG 60
           L +V KMKKGGIKCK+EE+ E K S+ES +S T+AA+   VVA AGPA ALVDERLSTEG
Sbjct: 30  LPVVQKMKKGGIKCKLEEKQEGKRSKESLISGTVAAMATTVVAMAGPAVALVDERLSTEG 89

Query: 61  TGLPFGLSNNLLAWILAGVFALIWALYFVYASSLDEDEDSGLSL 105
           TGLPFGLSNNLL WILAG+F  IWALYFVYASSL+EDEDSGLSL
Sbjct: 90  TGLPFGLSNNLLGWILAGMFGFIWALYFVYASSLEEDEDSGLSL 133

[Close]

BLAST of MS005289 vs. ExPASy TrEMBL
Match: A0A1S3C2B0 (PSII 6.1 kDa protein OS=Cucumis melo OX=3656 GN=LOC103496095 PE=3 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 4.7e-33
Identity = 80/99 (80.81%), Postives = 85/99 (85.86%), Query Frame = 0


Query: 6   KMKKGGIKCKVEERPECKSSRESTVSVTMAAVVAAVVAAAGPAAALVDERLSTEGTGLPF 65
           KMK G IKCKVEE+PE K S ES V  T+AAV  A +AAAGPA ALVDER+STEGTGLPF
Sbjct: 35  KMKMGRIKCKVEEKPEGKKSEESMVPGTVAAVAMAALAAAGPAVALVDERMSTEGTGLPF 94

Query: 66  GLSNNLLAWILAGVFALIWALYFVYASSLDEDEDSGLSL 105
           GLSNNLL WILAG+F LIWALYFVYASSLDEDEDSGLSL
Sbjct: 95  GLSNNLLGWILAGMFGLIWALYFVYASSLDEDEDSGLSL 133

[Close]

BLAST of MS005289 vs. ExPASy TrEMBL
Match: A0A1S4E257 (PSII 6.1 kDa protein OS=Cucumis melo OX=3656 GN=LOC103496095 PE=3 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 4.7e-33
Identity = 80/99 (80.81%), Postives = 85/99 (85.86%), Query Frame = 0


Query: 6   KMKKGGIKCKVEERPECKSSRESTVSVTMAAVVAAVVAAAGPAAALVDERLSTEGTGLPF 65
           KMK G IKCKVEE+PE K S ES V  T+AAV  A +AAAGPA ALVDER+STEGTGLPF
Sbjct: 57  KMKMGRIKCKVEEKPEGKKSEESMVPGTVAAVAMAALAAAGPAVALVDERMSTEGTGLPF 116

Query: 66  GLSNNLLAWILAGVFALIWALYFVYASSLDEDEDSGLSL 105
           GLSNNLL WILAG+F LIWALYFVYASSLDEDEDSGLSL
Sbjct: 117 GLSNNLLGWILAGMFGLIWALYFVYASSLDEDEDSGLSL 155

[Close]

BLAST of MS005289 vs. TAIR 10
Match: AT2G30570.1 (photosystem II reaction center W )

HSP 1 Score: 109.8 bits (273), Expect = 1.4e-24
Identity = 65/111 (58.56%), Postives = 78/111 (70.27%), Query Frame = 0


Query: 1   LLMVPKM--KKGGIKCKVEERPECKSSRESTVSVTMAAVVAAVVAA-----AGPAAALVD 60
           +L +P M  KKGG++C +E       +++  VSV  A V AA  AA     + PA ALVD
Sbjct: 30  VLGLPPMGKKKGGVRCSME-------TKQGNVSVMGAGVSAAATAALTAVMSNPAMALVD 89

Query: 61  ERLSTEGTGLPFGLSNNLLAWILAGVFALIWALYFVYASSLDEDEDSGLSL 105
           ER+STEGTGLPFGLSNNLL WIL GVF LIW  +FVY SSL+EDE+SGLSL
Sbjct: 90  ERMSTEGTGLPFGLSNNLLGWILFGVFGLIWTFFFVYTSSLEEDEESGLSL 133

The following BLAST results are available for this feature:

BLAST of MS005289 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143021.1	1.7e-45	99.04	photosystem II reaction center W protein, chloroplastic-like [Momordica charanti...	[more]
XP_038890119.1	8.5e-37	84.62	photosystem II reaction center W protein, chloroplastic-like [Benincasa hispida]	[more]
XP_022966002.1	2.6e-33	76.92	photosystem II reaction center W protein, chloroplastic-like [Cucurbita maxima]	[more]
XP_023521133.1	3.4e-33	76.92	photosystem II reaction center W protein, chloroplastic-like [Cucurbita pepo sub...	[more]
XP_022937622.1	4.4e-33	76.92	photosystem II reaction center W protein, chloroplastic-like [Cucurbita moschata...	[more]

BLAST of MS005289 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q41387	2.3e-24	58.88	Photosystem II reaction center W protein, chloroplastic OS=Spinacia oleracea OX=...	[more]
Q39194	1.9e-23	58.56	Photosystem II reaction center W protein, chloroplastic OS=Arabidopsis thaliana ...	[more]
Q5ZBY9	4.1e-18	53.19	Photosystem II reaction center W protein, chloroplastic OS=Oryza sativa subsp. j...	[more]
Q9SPI9	1.6e-09	40.57	Photosystem II reaction center W protein, chloroplastic OS=Chlamydomonas reinhar...	[more]

BLAST of MS005289 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CNY5	8.3e-46	99.04	PSII 6.1 kDa protein OS=Momordica charantia OX=3673 GN=LOC111013009 PE=3 SV=1	[more]
A0A6J1HLU7	1.2e-33	76.92	PSII 6.1 kDa protein OS=Cucurbita maxima OX=3661 GN=LOC111465720 PE=3 SV=1	[more]
A0A6J1FBR0	2.1e-33	76.92	PSII 6.1 kDa protein OS=Cucurbita moschata OX=3662 GN=LOC111443974 PE=3 SV=1	[more]
A0A1S3C2B0	4.7e-33	80.81	PSII 6.1 kDa protein OS=Cucumis melo OX=3656 GN=LOC103496095 PE=3 SV=1	[more]
A0A1S4E257	4.7e-33	80.81	PSII 6.1 kDa protein OS=Cucumis melo OX=3656 GN=LOC103496095 PE=3 SV=1	[more]

BLAST of MS005289 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G30570.1	1.4e-24	58.56	photosystem II reaction center W	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009806	Photosystem II PsbW, class 2	PFAM	PF07123	PsbW	coord: 4..104 e-value: 3.1E-41 score: 140.5
IPR009806	Photosystem II PsbW, class 2	PANTHER	PTHR34552	PHOTOSYSTEM II REACTION CENTER W PROTEIN, CHLOROPLASTIC	coord: 3..104
None	No IPR available	PANTHER	PTHR34552:SF5	PHOTOSYSTEM II REACTION CENTER W PROTEIN, CHLOROPLASTIC-LIKE	coord: 3..104

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS005289.1	MS005289.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015979	photosynthesis
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0009523	photosystem II