MS005267 (gene) Bitter gourd (TR) v1

Overview


Name	MS005267
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	VQ domain-containing protein
Location	scaffold83: 431675 .. 432073 (-)
RNA-Seq Expression	MS005267
Synteny	MS005267

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATAACCTTAGAAGAAGCCTTCCTGATCATCATCGTCATCACCAAAGAAGCCAAGCTCCCAATAGAGCAATAATGGAGAAGCCCATCAAAGTCAAGTACATTTCGAACCCGATGATGGTGAAGGCGAACTCCGAATCAGAGTTTCGAGAAATAGTTCAGAGGCTTACCGGCCAGAACTCGCCCGATGATGCTTTTGAATCACCAGCTTTTGGAGGAGAAGAAGAAGAATTAAACCATGCTTTTTATCCTCCTCCGCCGTCTACGGCGGTGTCGGCTGACCCTAAGGGTTATTATGGCTTGGTCTCCGGCAAGGTCGGCGAGCTCGGCGAAGGCTGCTTCCGGCAAGATGAAATGCAGGGTTTTTCTGGGTTTCAAGCTTCTTGCGTTAATACATAT

mRNA sequence

ATGGATAACCTTAGAAGAAGCCTTCCTGATCATCATCGTCATCACCAAAGAAGCCAAGCTCCCAATAGAGCAATAATGGAGAAGCCCATCAAAGTCAAGTACATTTCGAACCCGATGATGGTGAAGGCGAACTCCGAATCAGAGTTTCGAGAAATAGTTCAGAGGCTTACCGGCCAGAACTCGCCCGATGATGCTTTTGAATCACCAGCTTTTGGAGGAGAAGAAGAAGAATTAAACCATGCTTTTTATCCTCCTCCGCCGTCTACGGCGGTGTCGGCTGACCCTAAGGGTTATTATGGCTTGGTCTCCGGCAAGGTCGGCGAGCTCGGCGAAGGCTGCTTCCGGCAAGATGAAATGCAGGGTTTTTCTGGGTTTCAAGCTTCTTGCGTTAATACATAT

Coding sequence (CDS)

ATGGATAACCTTAGAAGAAGCCTTCCTGATCATCATCGTCATCACCAAAGAAGCCAAGCTCCCAATAGAGCAATAATGGAGAAGCCCATCAAAGTCAAGTACATTTCGAACCCGATGATGGTGAAGGCGAACTCCGAATCAGAGTTTCGAGAAATAGTTCAGAGGCTTACCGGCCAGAACTCGCCCGATGATGCTTTTGAATCACCAGCTTTTGGAGGAGAAGAAGAAGAATTAAACCATGCTTTTTATCCTCCTCCGCCGTCTACGGCGGTGTCGGCTGACCCTAAGGGTTATTATGGCTTGGTCTCCGGCAAGGTCGGCGAGCTCGGCGAAGGCTGCTTCCGGCAAGATGAAATGCAGGGTTTTTCTGGGTTTCAAGCTTCTTGCGTTAATACATAT

Protein sequence

MDNLRRSLPDHHRHHQRSQAPNRAIMEKPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPDDAFESPAFGGEEEELNHAFYPPPPSTAVSADPKGYYGLVSGKVGELGEGCFRQDEMQGFSGFQASCVNTY

Homology

[Close]

BLAST of MS005267 vs. NCBI nr
Match: XP_022142960.1 (sigma factor binding protein 1, chloroplastic-like [Momordica charantia])

HSP 1 Score: 216.1 bits (549), Expect = 1.9e-52
Identity = 106/108 (98.15%), Postives = 106/108 (98.15%), Query Frame = 0


Query: 26  MEKPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPDDAFESPAFGGEEEELNHAFYPP 85
           MEKPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPDDAFESPAFGG EEELNHAFYPP
Sbjct: 1   MEKPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPDDAFESPAFGG-EEELNHAFYPP 60

Query: 86  PPSTAVSADPKGYYGLVSGKVGELGEGCFRQDEMQGFSGFQASCVNTY 134
           PPSTAVS DPKGYYGLVSGKVGELGEGCFRQDEMQGFSGFQASCVNTY
Sbjct: 61  PPSTAVSTDPKGYYGLVSGKVGELGEGCFRQDEMQGFSGFQASCVNTY 107

[Close]

BLAST of MS005267 vs. NCBI nr
Match: KAA0063579.1 (VQ motif-containing protein [Cucumis melo var. makuwa] >TYJ97906.1 VQ motif-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 116.7 bits (291), Expect = 1.5e-22
Identity = 74/148 (50.00%), Postives = 94/148 (63.51%), Query Frame = 0


Query: 1   MDNLRRSLPDHHRHHQRSQAPNRAI----MEKPIKVKYISNPMMVKANSESEFREIVQRL 60
           M+NL  +LP    H + SQ  N+        KPIKVKYIS+PMMVKAN+ESEFR IVQ+L
Sbjct: 1   MENLITTLPTDFHHRKVSQNSNKPTTIDQQNKPIKVKYISSPMMVKANNESEFRAIVQKL 60

Query: 61  TGQNSPDDAFESPAFGGEEEELNHAFYPPPPSTA---VSADPKGYYGLVSG------KVG 120
           TGQ+SPD       F    ++ NHAFYPPPPS++   VS DP+  YG  SG      ++ 
Sbjct: 61  TGQHSPD---HDANFDQSVQDFNHAFYPPPPSSSTPGVSFDPRDCYGSASGDVLFDDRIE 120

Query: 121 ELGEGCFRQDEMQG-FSGFQA-SCVNTY 134
           +  EG + + EM+G FSGFQA SCVN +
Sbjct: 121 DGDEGRYWRGEMEGSFSGFQASSCVNIW 145

[Close]

BLAST of MS005267 vs. NCBI nr
Match: KAE8651551.1 (hypothetical protein Csa_019210 [Cucumis sativus])

HSP 1 Score: 110.2 bits (274), Expect = 1.4e-20
Identity = 69/141 (48.94%), Postives = 93/141 (65.96%), Query Frame = 0


Query: 7   SLPDHHRHHQRSQAPNRAIME---KPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPD 66
           +LP +  H + SQ  N+A ++   KPIKVKYIS+PMMVKAN+ESEFR IVQ+LTGQ+SPD
Sbjct: 6   TLPSNFHHKKVSQVSNKATIDHQNKPIKVKYISSPMMVKANNESEFRAIVQKLTGQHSPD 65

Query: 67  DAFESPAFGGEEEELNHAFYPPPPSTA--VSADPKGYYG------LVSGKVGELGEGCFR 126
              +S       ++ NH FYPPPPS++  VS DPK  YG      ++  ++ +  EG + 
Sbjct: 66  HDDQS------VQDFNHVFYPPPPSSSPGVSFDPKDCYGNSASGDVLFDRIEDGDEGRYW 125

Query: 127 QDEMQ--GFSGFQA-SCVNTY 134
           + EM+   FSGFQA SCVN +
Sbjct: 126 RGEMELGSFSGFQASSCVNIW 140

[Close]

BLAST of MS005267 vs. NCBI nr
Match: XP_038877668.1 (sigma factor binding protein 1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 105.9 bits (263), Expect = 2.7e-19
Identity = 66/109 (60.55%), Postives = 75/109 (68.81%), Query Frame = 0


Query: 28  KPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPDDAFESPAFGGEEEELNHAFYPPPP 87
           KPIKVKYIS+PMMVKAN+ESEFR IVQ+LTGQ+SP DAF+  A     E+ N  FYPPP 
Sbjct: 5   KPIKVKYISSPMMVKANNESEFRAIVQKLTGQHSPHDAFDQSA-----EDFNQVFYPPPS 64

Query: 88  STAV-SADPKGYYGLVSGKVGELGEGCFRQDEMQG-FSGFQA-SCVNTY 134
           S A  S DPK  Y   SG V    +G     EM+G FSGFQA SCVNT+
Sbjct: 65  SMAAPSFDPKDCYASASGDVLFDWQG-----EMEGSFSGFQASSCVNTW 103

[Close]

BLAST of MS005267 vs. NCBI nr
Match: XP_022938305.1 (sigma factor binding protein 1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 100.5 bits (249), Expect = 1.1e-17
Identity = 69/140 (49.29%), Postives = 85/140 (60.71%), Query Frame = 0


Query: 1   MDNLRRSLPDHHRHHQRSQAPNRAIM-EKPIKVKYISNPMMVKANSESEFREIVQRLTGQ 60
           M+NL +SLP+H RH +   +  RA M  KPIKVKYIS+PMMVKAN+E EFR IVQ+LTGQ
Sbjct: 1   MENL-KSLPNHLRHEKLEASNYRATMANKPIKVKYISSPMMVKANNEFEFRAIVQKLTGQ 60

Query: 61  NSPDDAFESPAFGGEEEELNHAFYPPPPSTAVSADPKGYYGLVSGK------VGELGEGC 120
           +S DDAF+        +E NHAF  PP ST  S D K  Y LVS        VGE+ +  
Sbjct: 61  HSADDAFDR-----SPQEFNHAFC-PPSSTVTSFDSKDCYDLVSSDAYDPIGVGEMKDSL 120

Query: 121 FRQDEMQGFSGFQASCVNTY 134
           +         GFQAS V+ +
Sbjct: 121 Y---------GFQASYVDVW 124

[Close]

BLAST of MS005267 vs. ExPASy Swiss-Prot
Match: Q9LDH1 (Sigma factor binding protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SIB1 PE=1 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.5e-04
Identity = 40/101 (39.60%), Postives = 53/101 (52.48%), Query Frame = 0


Query: 28  KPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPDDAFESPAFGGEEEELNHAFYPPPP 87
           KPIKV+YISNPM V+    S+FRE+VQ LTGQ++ D   E P +    ++  H   PP  
Sbjct: 38  KPIKVRYISNPMRVQ-TCASKFRELVQELTGQDAVDLQPE-PIYSPSSDD--HNLSPPAE 97

Query: 88  STAVSADPKGYYGLVSGKVGE--LGEGCFRQDEMQ-GFSGF 126
           + A     +  +G       E    E  F  D+M  GFSGF
Sbjct: 98  NLAPRVLHQEPFGERDSDCYEPLNAEDMFLPDQMSAGFSGF 134

[Close]

BLAST of MS005267 vs. ExPASy Swiss-Prot
Match: O80669 (Sigma factor binding protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SIB2 PE=1 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 4.4e-04
Identity = 23/36 (63.89%), Postives = 29/36 (80.56%), Query Frame = 0


Query: 28 KPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPD 64
          KPIKV+YISNPM V+    S+FRE+VQ LTGQ++ D
Sbjct: 35 KPIKVRYISNPMRVE-TCPSKFRELVQELTGQDAAD 69

[Close]

BLAST of MS005267 vs. ExPASy TrEMBL
Match: A0A6J1CME4 (sigma factor binding protein 1, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111012954 PE=4 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 9.0e-53
Identity = 106/108 (98.15%), Postives = 106/108 (98.15%), Query Frame = 0


Query: 26  MEKPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPDDAFESPAFGGEEEELNHAFYPP 85
           MEKPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPDDAFESPAFGG EEELNHAFYPP
Sbjct: 1   MEKPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPDDAFESPAFGG-EEELNHAFYPP 60

Query: 86  PPSTAVSADPKGYYGLVSGKVGELGEGCFRQDEMQGFSGFQASCVNTY 134
           PPSTAVS DPKGYYGLVSGKVGELGEGCFRQDEMQGFSGFQASCVNTY
Sbjct: 61  PPSTAVSTDPKGYYGLVSGKVGELGEGCFRQDEMQGFSGFQASCVNTY 107

[Close]

BLAST of MS005267 vs. ExPASy TrEMBL
Match: A0A5A7VDH6 (VQ motif-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G001220 PE=4 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 7.4e-23
Identity = 74/148 (50.00%), Postives = 94/148 (63.51%), Query Frame = 0


Query: 1   MDNLRRSLPDHHRHHQRSQAPNRAI----MEKPIKVKYISNPMMVKANSESEFREIVQRL 60
           M+NL  +LP    H + SQ  N+        KPIKVKYIS+PMMVKAN+ESEFR IVQ+L
Sbjct: 1   MENLITTLPTDFHHRKVSQNSNKPTTIDQQNKPIKVKYISSPMMVKANNESEFRAIVQKL 60

Query: 61  TGQNSPDDAFESPAFGGEEEELNHAFYPPPPSTA---VSADPKGYYGLVSG------KVG 120
           TGQ+SPD       F    ++ NHAFYPPPPS++   VS DP+  YG  SG      ++ 
Sbjct: 61  TGQHSPD---HDANFDQSVQDFNHAFYPPPPSSSTPGVSFDPRDCYGSASGDVLFDDRIE 120

Query: 121 ELGEGCFRQDEMQG-FSGFQA-SCVNTY 134
           +  EG + + EM+G FSGFQA SCVN +
Sbjct: 121 DGDEGRYWRGEMEGSFSGFQASSCVNIW 145

[Close]

BLAST of MS005267 vs. ExPASy TrEMBL
Match: A0A0A0LFF9 (VQ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G010160 PE=4 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 6.9e-21
Identity = 69/141 (48.94%), Postives = 93/141 (65.96%), Query Frame = 0


Query: 7   SLPDHHRHHQRSQAPNRAIME---KPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPD 66
           +LP +  H + SQ  N+A ++   KPIKVKYIS+PMMVKAN+ESEFR IVQ+LTGQ+SPD
Sbjct: 6   TLPSNFHHKKVSQVSNKATIDHQNKPIKVKYISSPMMVKANNESEFRAIVQKLTGQHSPD 65

Query: 67  DAFESPAFGGEEEELNHAFYPPPPSTA--VSADPKGYYG------LVSGKVGELGEGCFR 126
              +S       ++ NH FYPPPPS++  VS DPK  YG      ++  ++ +  EG + 
Sbjct: 66  HDDQS------VQDFNHVFYPPPPSSSPGVSFDPKDCYGNSASGDVLFDRIEDGDEGRYW 125

Query: 127 QDEMQ--GFSGFQA-SCVNTY 134
           + EM+   FSGFQA SCVN +
Sbjct: 126 RGEMELGSFSGFQASSCVNIW 140

[Close]

BLAST of MS005267 vs. ExPASy TrEMBL
Match: A0A6J1FJE0 (sigma factor binding protein 1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111444440 PE=4 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 5.5e-18
Identity = 69/140 (49.29%), Postives = 85/140 (60.71%), Query Frame = 0


Query: 1   MDNLRRSLPDHHRHHQRSQAPNRAIM-EKPIKVKYISNPMMVKANSESEFREIVQRLTGQ 60
           M+NL +SLP+H RH +   +  RA M  KPIKVKYIS+PMMVKAN+E EFR IVQ+LTGQ
Sbjct: 1   MENL-KSLPNHLRHEKLEASNYRATMANKPIKVKYISSPMMVKANNEFEFRAIVQKLTGQ 60

Query: 61  NSPDDAFESPAFGGEEEELNHAFYPPPPSTAVSADPKGYYGLVSGK------VGELGEGC 120
           +S DDAF+        +E NHAF  PP ST  S D K  Y LVS        VGE+ +  
Sbjct: 61  HSADDAFDR-----SPQEFNHAFC-PPSSTVTSFDSKDCYDLVSSDAYDPIGVGEMKDSL 120

Query: 121 FRQDEMQGFSGFQASCVNTY 134
           +         GFQAS V+ +
Sbjct: 121 Y---------GFQASYVDVW 124

[Close]

BLAST of MS005267 vs. ExPASy TrEMBL
Match: A0A3P6BV84 (VQ domain-containing protein (Fragment) OS=Brassica oleracea OX=3712 GN=BOLC4T25224H PE=4 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 9.7e-07
Identity = 48/110 (43.64%), Postives = 60/110 (54.55%), Query Frame = 0


Query: 19  QAPNRAIMEKPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPDDAFESPAFGGEEEEL 78
           Q   RA   KPIKV+YISNPM VK    S+FRE+VQ LTGQ++ D   E P F       
Sbjct: 26  QMKKRASRNKPIKVRYISNPMRVK-TCASKFRELVQELTGQDAVDLELE-PEFSPSAVS- 85

Query: 79  NHAFYPPPPSTAVSADP--KGYYGLVSGKVGEL-GEGCFRQDEMQGFSGF 126
           +H+  PPPP T     P  + +  LV+G    L GE  F     +GFSG+
Sbjct: 86  DHSPTPPPPETFAPHIPHQEPFDDLVAGYYEPLDGEEMFLSQMSRGFSGY 132

[Close]

BLAST of MS005267 vs. TAIR 10
Match: AT3G56710.1 (sigma factor binding protein 1 )

HSP 1 Score: 47.4 bits (111), Expect = 1.1e-05
Identity = 40/101 (39.60%), Postives = 53/101 (52.48%), Query Frame = 0


Query: 28  KPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPDDAFESPAFGGEEEELNHAFYPPPP 87
           KPIKV+YISNPM V+    S+FRE+VQ LTGQ++ D   E P +    ++  H   PP  
Sbjct: 38  KPIKVRYISNPMRVQ-TCASKFRELVQELTGQDAVDLQPE-PIYSPSSDD--HNLSPPAE 97

Query: 88  STAVSADPKGYYGLVSGKVGE--LGEGCFRQDEMQ-GFSGF 126
           + A     +  +G       E    E  F  D+M  GFSGF
Sbjct: 98  NLAPRVLHQEPFGERDSDCYEPLNAEDMFLPDQMSAGFSGF 134

[Close]

BLAST of MS005267 vs. TAIR 10
Match: AT2G41180.1 (VQ motif-containing protein )

HSP 1 Score: 45.8 bits (107), Expect = 3.1e-05
Identity = 23/36 (63.89%), Postives = 29/36 (80.56%), Query Frame = 0


Query: 28 KPIKVKYISNPMMVKANSESEFREIVQRLTGQNSPD 64
          KPIKV+YISNPM V+    S+FRE+VQ LTGQ++ D
Sbjct: 35 KPIKVRYISNPMRVE-TCPSKFRELVQELTGQDAAD 69

The following BLAST results are available for this feature:

BLAST of MS005267 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142960.1	1.9e-52	98.15	sigma factor binding protein 1, chloroplastic-like [Momordica charantia]	[more]
KAA0063579.1	1.5e-22	50.00	VQ motif-containing protein [Cucumis melo var. makuwa] >TYJ97906.1 VQ motif-cont...	[more]
KAE8651551.1	1.4e-20	48.94	hypothetical protein Csa_019210 [Cucumis sativus]	[more]
XP_038877668.1	2.7e-19	60.55	sigma factor binding protein 1, chloroplastic-like [Benincasa hispida]	[more]
XP_022938305.1	1.1e-17	49.29	sigma factor binding protein 1, chloroplastic-like [Cucurbita moschata]	[more]

BLAST of MS005267 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LDH1	1.5e-04	39.60	Sigma factor binding protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN...	[more]
O80669	4.4e-04	63.89	Sigma factor binding protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN...	[more]

BLAST of MS005267 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CME4	9.0e-53	98.15	sigma factor binding protein 1, chloroplastic-like OS=Momordica charantia OX=367...	[more]
A0A5A7VDH6	7.4e-23	50.00	VQ motif-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A0A0LFF9	6.9e-21	48.94	VQ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G010160 PE=4 SV=...	[more]
A0A6J1FJE0	5.5e-18	49.29	sigma factor binding protein 1, chloroplastic-like OS=Cucurbita moschata OX=3662...	[more]
A0A3P6BV84	9.7e-07	43.64	VQ domain-containing protein (Fragment) OS=Brassica oleracea OX=3712 GN=BOLC4T25...	[more]

BLAST of MS005267 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G56710.1	1.1e-05	39.60	sigma factor binding protein 1	[more]
AT2G41180.1	3.1e-05	63.89	VQ motif-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008889	VQ	PFAM	PF05678	VQ	coord: 35..63 e-value: 3.0E-9 score: 36.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..24
IPR039335	Sigma factor binding protein 1/2	PANTHER	PTHR33624	SIGMA FACTOR BINDING PROTEIN 1, CHLOROPLASTIC	coord: 11..76

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS005267.1	MS005267.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051091	positive regulation of DNA-binding transcription factor activity