MS005230 (gene) Bitter gourd (TR) v1

Overview
NameMS005230
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionE3 ubiquitin-protein ligase EL5
Locationscaffold83: 161478 .. 162964 (+)
RNA-Seq ExpressionMS005230
SyntenyMS005230
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGGAATGTTGCCGGGAGTCGAATGCGCGAGAAGGCGGCGGTTCCACCAGAGCATAACCTCGTCGGACTCGCCGTCCACGGCGGCGAAACTGGGATCCACCAGAAGGCCCTCCTTCTGTTTGTACACCAGCAACCGCGATTTCCACCTCACTTCATCTTCTACACAGGTAAAGAAAAAAAATCTACATACAATTAAATTCCATCCACATCCTTGTTCCAATTATCTGTTTGTTTCCTGAGAAAAATCTGGTGAATGATTTTCAAATTTAAACAAAAATTAAGCAACGTAGCTGGTCGAATCAAGCTTTCCAGAACGATAAACTGGGTGAAATTGCGAGAGAAGCCAAAGAGAGGTTGGATGAGAGGTTGAAGACACGCAGAAAACCAGAGACTGAAACTGTAAGGTAATTTCTCCTCGGTGAGCTATTTTTCTCTCTCTCTCTTTTTTGTTTCATTGAGAATTTTATTGATTATCATTAAAAATTAAAAGCGTCTCGCGGATAATAAATAATCTCTATGCTTATGCTTAATATCATAAATAATAGTTACATGTATAAAAAGATTTAAGTACCATTAATTTTTTAAAATAATCATCCAAGTGTTAATTGAATGATTAATGAGAATGTGAGTAATTTTTACGGTTTTGGTCTATGTCAAAATCACTCTCAAAGACACCCCGAGTAAAATTGCACAATTATTTCACAAAATTTTGTAAGGTTTGTCTTTTATTTGGGTATTTAGTCAACTATATTAATATGTTAGGTTGAGTAAAAATTAAAAGGACTATAAGACTAAACTTGTAATTTACTTAAAATATTTTAGGTTAAGCTATTAGTTTAGTCTCTAAATTTTCAAGATTGTTTTTAATGCGTCCTTAAATTTAAAAAAAAAAAATCTAATAAGTTGCTAGATCTTCAACTTTGTGTCAAATACTTATTAGACATAAAACTAAAATAATGATATTAAGGTATCAATAAGTAGGTATTTCGTGATACTAGAAGAGTACAAATACTTTTTTCGTTTATTGTCAACCATGTTAGTATTGTATTAAAAATAATGTAAGGTCCGGTAACAAACAAAATCATAGTGTGAATAGAGAGAATATAGCACCGCATAGTACTATCCGTTAAAGCACCTAAGCTTAGGTATGCATTTGATGGAATTTTGAAAGTTTTATAACTTGATATGGTACAATTATAGGAAGAGTAGGAGTAAGAGGGAAGAAGAAGTAAGGTCAAAAGGGTCAGAAAAGGAAGGGGAAATGACAAAAGGCAAAGAAGACGAGAGAAAGAAGAAGTTGATGAGTTGGGGAAAGTGGAAGGCGTGGGAGCAAAAGGAGTGCGCCGTTTGCCTAGAAGGGTTCAGGGGTGGCGAGCCGGTGGTCCATCTGCCATGCGCCCACAAGTTCCATTCCTCCTGCCTCGTCCCTTGGCTCCAAACCAACTCCCATTGCCCTTCTTGCAGATTCCCCATTTCCGCTCCT

mRNA sequence

ATGGCGGGAATGTTGCCGGGAGTCGAATGCGCGAGAAGGCGGCGGTTCCACCAGAGCATAACCTCGTCGGACTCGCCGTCCACGGCGGCGAAACTGGGATCCACCAGAAGGCCCTCCTTCTGTTTGTACACCAGCAACCGCGATTTCCACCTCACTTCATCTTCTACACAGCAACGTAGCTGGTCGAATCAAGCTTTCCAGAACGATAAACTGGGTGAAATTGCGAGAGAAGCCAAAGAGAGGTTGGATGAGAGGTTGAAGACACGCAGAAAACCAGAGACTGAAACTGTAAGTAAGAGGGAAGAAGAAGTAAGGTCAAAAGGGTCAGAAAAGGAAGGGGAAATGACAAAAGGCAAAGAAGACGAGAGAAAGAAGAAGTTGATGAGTTGGGGAAAGTGGAAGGCGTGGGAGCAAAAGGAGTGCGCCGTTTGCCTAGAAGGGTTCAGGGGTGGCGAGCCGGTGGTCCATCTGCCATGCGCCCACAAGTTCCATTCCTCCTGCCTCGTCCCTTGGCTCCAAACCAACTCCCATTGCCCTTCTTGCAGATTCCCCATTTCCGCTCCT

Coding sequence (CDS)

ATGGCGGGAATGTTGCCGGGAGTCGAATGCGCGAGAAGGCGGCGGTTCCACCAGAGCATAACCTCGTCGGACTCGCCGTCCACGGCGGCGAAACTGGGATCCACCAGAAGGCCCTCCTTCTGTTTGTACACCAGCAACCGCGATTTCCACCTCACTTCATCTTCTACACAGCAACGTAGCTGGTCGAATCAAGCTTTCCAGAACGATAAACTGGGTGAAATTGCGAGAGAAGCCAAAGAGAGGTTGGATGAGAGGTTGAAGACACGCAGAAAACCAGAGACTGAAACTGTAAGTAAGAGGGAAGAAGAAGTAAGGTCAAAAGGGTCAGAAAAGGAAGGGGAAATGACAAAAGGCAAAGAAGACGAGAGAAAGAAGAAGTTGATGAGTTGGGGAAAGTGGAAGGCGTGGGAGCAAAAGGAGTGCGCCGTTTGCCTAGAAGGGTTCAGGGGTGGCGAGCCGGTGGTCCATCTGCCATGCGCCCACAAGTTCCATTCCTCCTGCCTCGTCCCTTGGCTCCAAACCAACTCCCATTGCCCTTCTTGCAGATTCCCCATTTCCGCTCCT

Protein sequence

MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHLTSSSTQQRSWSNQAFQNDKLGEIAREAKERLDERLKTRRKPETETVSKREEEVRSKGSEKEGEMTKGKEDERKKKLMSWGKWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNSHCPSCRFPISAP
Homology
BLAST of MS005230 vs. NCBI nr
Match: XP_022143236.1 (E3 ubiquitin-protein ligase EL5 [Momordica charantia])

HSP 1 Score: 368.2 bits (944), Expect = 4.1e-98
Identity = 186/192 (96.88%), Postives = 188/192 (97.92%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHLTSSSTQQRS 60
           MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHLTSSSTQQRS
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHLTSSSTQQRS 60

Query: 61  WSNQAFQNDKLGEIAREAKERLDERLKTRRKPETETV----SKREEEVRSKGSEKEGEMT 120
           WSNQAFQNDKLGEIAREAKERLDERLKTRR+PETETV    SKREEEVRSKGSEKEGEMT
Sbjct: 61  WSNQAFQNDKLGEIAREAKERLDERLKTRRRPETETVRKSRSKREEEVRSKGSEKEGEMT 120

Query: 121 KGKEDERKKKLMSWGKWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNS 180
           KGKE+ERKKKLMSWGKWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNS
Sbjct: 121 KGKEEERKKKLMSWGKWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNS 180

Query: 181 HCPSCRFPISAP 189
           HCPSCRFPISAP
Sbjct: 181 HCPSCRFPISAP 192

BLAST of MS005230 vs. NCBI nr
Match: XP_004139268.1 (probable E3 ubiquitin-protein ligase RHY1A [Cucumis sativus] >KGN60748.1 hypothetical protein Csa_019469 [Cucumis sativus])

HSP 1 Score: 266.5 bits (680), Expect = 1.7e-67
Identity = 143/200 (71.50%), Postives = 160/200 (80.00%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHL-TSSSTQQR 60
           MAGMLPGVECARRRRFHQSI SSDSP TA K GSTRRPSFCLYTSNRDFHL +SSS+QQR
Sbjct: 1   MAGMLPGVECARRRRFHQSINSSDSPFTAGKSGSTRRPSFCLYTSNRDFHLISSSSSQQR 60

Query: 61  SWSNQAFQNDKLGEIAREAKERLDERLKTRRKPETETVSKREEEVRSK-------GSEKE 120
           S +NQA+QNDKLGEIAREAKERLDERL+T RKPE        EEV+ K       G +KE
Sbjct: 61  SLTNQAYQNDKLGEIAREAKERLDERLRTHRKPENSRKKNNNEEVKGKESREKNNGEKKE 120

Query: 121 GEMTKGKEDERKKKLMSWG----KWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCL 180
            + TKGKE+E KK+  SWG    KWKA EQ+EC+VCLE FR  EP++HLPCAHKFHS+CL
Sbjct: 121 MKNTKGKEEETKKR-FSWGRKLMKWKASEQEECSVCLERFRLSEPLLHLPCAHKFHSTCL 180

Query: 181 VPWLQTNSHCPSCRFPISAP 189
           VPWLQ N+HCP CRFPI+ P
Sbjct: 181 VPWLQANAHCPCCRFPIAVP 199

BLAST of MS005230 vs. NCBI nr
Match: KAG7030344.1 (putative E3 ubiquitin-protein ligase RHY1A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 265.4 bits (677), Expect = 3.8e-67
Identity = 140/189 (74.07%), Postives = 158/189 (83.60%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHLTSSS--TQQ 60
           MAGMLPGVECARRRRFHQSIT SD+PSTAAKLGSTRRPSFCLYTSNRDFH+TSSS  +QQ
Sbjct: 1   MAGMLPGVECARRRRFHQSITLSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCSQQ 60

Query: 61  RSWSNQAFQNDKLGEIAREAKERLDERLKTRRKPETETVSKREEEVRSKGSEKEGEMTKG 120
           RS S+QA+QN+KLGEIAREAKERLDERL+T RKPE     +++     K S ++ E  KG
Sbjct: 61  RSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPEN---PRKKSGEGLKNSLEKKEKAKG 120

Query: 121 KEDERKKKLMSWG---KWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTN 180
           KE+   KK  +WG   KWKA EQ+ECAVCLEGFR GEP+VHLPCAHKFHS+CLVPWLQTN
Sbjct: 121 KEESITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTN 180

Query: 181 SHCPSCRFP 185
           +HCP CRFP
Sbjct: 181 AHCPCCRFP 186

BLAST of MS005230 vs. NCBI nr
Match: XP_022965966.1 (probable E3 ubiquitin-protein ligase RHY1A isoform X1 [Cucurbita maxima] >XP_022965967.1 probable E3 ubiquitin-protein ligase RHY1A isoform X1 [Cucurbita maxima] >XP_022965968.1 probable E3 ubiquitin-protein ligase RHY1A isoform X1 [Cucurbita maxima])

HSP 1 Score: 261.5 bits (667), Expect = 5.4e-66
Identity = 138/190 (72.63%), Postives = 149/190 (78.42%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHLTSSSTQQRS 60
           MAGMLPGVECARRRRFHQSITSS+S STAAKLGSTRR SFCLYTSNRDFHLTSSS+QQR 
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSESASTAAKLGSTRRSSFCLYTSNRDFHLTSSSSQQRC 60

Query: 61  WSNQAFQNDKLGEIAREAKERLDERLKTRRKPETETVSKREEEVRSKGSEKEGEMTKGKE 120
            SN A+QN+KLG+ AREAKERLDERL+T RKPE        EEVR K      E  KGKE
Sbjct: 61  SSNHAYQNEKLGQAAREAKERLDERLRTHRKPENSRTKSSSEEVRLK-----KENVKGKE 120

Query: 121 DERKKKLMSWG---KWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNSH 180
           +   KK  SWG   KWKA EQ+EC VCLE FR  EP+VHLPCAHKFHS CLVPWLQ N+H
Sbjct: 121 ETTTKKRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQANAH 180

Query: 181 CPSCRFPISA 188
           CP CR+PI A
Sbjct: 181 CPCCRYPILA 185

BLAST of MS005230 vs. NCBI nr
Match: XP_038891102.1 (probable E3 ubiquitin-protein ligase RHY1A [Benincasa hispida])

HSP 1 Score: 260.8 bits (665), Expect = 9.3e-66
Identity = 145/195 (74.36%), Postives = 156/195 (80.00%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHLT-SSSTQQR 60
           MAGMLPGVECARRRRFHQSI SSDSPSTAAK GSTRRPSFCLYTSNRDFH T SSS+Q R
Sbjct: 1   MAGMLPGVECARRRRFHQSIASSDSPSTAAKSGSTRRPSFCLYTSNRDFHSTSSSSSQHR 60

Query: 61  SWSNQAFQNDKLGEIAREAKERLDERLKTRRKPETETVSKREEEVRSKG-SEKEG---EM 120
           S SNQA+QN+KLGEIAREAKERLDERL+T RKPE        E+V  KG SEK G   E 
Sbjct: 61  SSSNQAYQNEKLGEIAREAKERLDERLRTHRKPENSRRKSENEKVGWKGSSEKNGEEKER 120

Query: 121 TKGKEDE--RKKKLMSWG---KWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVP 180
           TKGKE+     KK  +WG   KW+A EQ+EC VCLE FR GEP+VHLPCAHKFHS+CLVP
Sbjct: 121 TKGKEESIIITKKRFNWGKLMKWRASEQEECTVCLERFRVGEPLVHLPCAHKFHSTCLVP 180

Query: 181 WLQTNSHCPSCRFPI 186
           WLQ NSHCP CRFPI
Sbjct: 181 WLQANSHCPCCRFPI 195

BLAST of MS005230 vs. ExPASy Swiss-Prot
Match: Q852U6 (Probable E3 ubiquitin-protein ligase RHY1A OS=Arabidopsis thaliana OX=3702 GN=RHY1A PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.5e-10
Identity = 25/50 (50.00%), Postives = 34/50 (68.00%), Query Frame = 0

Query: 137 EQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNSHCPSCRFPIS 187
           E ++C++CLE F  G+ ++ LPC H FHSSCL PWL+    CP CR  I+
Sbjct: 199 EMRDCSICLESFTKGDMLISLPCTHSFHSSCLNPWLRACGDCPCCRRAIA 248

BLAST of MS005230 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 4.4e-10
Identity = 23/49 (46.94%), Postives = 32/49 (65.31%), Query Frame = 0

Query: 137 EQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNSHCPSCRFPI 186
           E  +CAVC++ F  G  V  +PC H FH  CL+PWL+ ++ CP CRF +
Sbjct: 212 EMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFEL 260

BLAST of MS005230 vs. ExPASy Swiss-Prot
Match: Q9C1X4 (Uncharacterized RING finger protein P32A8.03c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAP32A8.03c PE=4 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.2e-09
Identity = 22/49 (44.90%), Postives = 32/49 (65.31%), Query Frame = 0

Query: 137 EQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNSHCPSCRFPI 186
           E+ EC +C+E F+  + V+ LPC H FH +C+ PWL+ N  C  CR P+
Sbjct: 392 EEGECTICMEMFKINDDVIQLPCKHYFHENCIKPWLRVNGTCAICRAPV 440

BLAST of MS005230 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 6.3e-09
Identity = 27/68 (39.71%), Postives = 39/68 (57.35%), Query Frame = 0

Query: 118 GKEDERKKKLMSWGKWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNSH 177
           G    +K+ + +    K  E   C+VCL+    G     +PC HKFHSSC++PWL+ +S 
Sbjct: 176 GTPPAKKEAVEALPTVKIEEVVSCSVCLDDLEVGSQAKQMPCEHKFHSSCILPWLELHSS 235

Query: 178 CPSCRFPI 186
           CP CRF +
Sbjct: 236 CPVCRFEL 243

BLAST of MS005230 vs. ExPASy Swiss-Prot
Match: Q91YL2 (E3 ubiquitin-protein ligase RNF126 OS=Mus musculus OX=10090 GN=Rnf126 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.1e-08
Identity = 24/43 (55.81%), Postives = 28/43 (65.12%), Query Frame = 0

Query: 140 ECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNSHCPSCR 183
           EC VC E +  GE V  LPC H FH SC+VPWL+ +  CP CR
Sbjct: 230 ECPVCKEDYALGESVRQLPCNHLFHDSCIVPWLEQHDSCPVCR 272

BLAST of MS005230 vs. ExPASy TrEMBL
Match: A0A6J1CPN1 (E3 ubiquitin-protein ligase EL5 OS=Momordica charantia OX=3673 GN=LOC111013152 PE=4 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 2.0e-98
Identity = 186/192 (96.88%), Postives = 188/192 (97.92%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHLTSSSTQQRS 60
           MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHLTSSSTQQRS
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHLTSSSTQQRS 60

Query: 61  WSNQAFQNDKLGEIAREAKERLDERLKTRRKPETETV----SKREEEVRSKGSEKEGEMT 120
           WSNQAFQNDKLGEIAREAKERLDERLKTRR+PETETV    SKREEEVRSKGSEKEGEMT
Sbjct: 61  WSNQAFQNDKLGEIAREAKERLDERLKTRRRPETETVRKSRSKREEEVRSKGSEKEGEMT 120

Query: 121 KGKEDERKKKLMSWGKWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNS 180
           KGKE+ERKKKLMSWGKWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNS
Sbjct: 121 KGKEEERKKKLMSWGKWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNS 180

Query: 181 HCPSCRFPISAP 189
           HCPSCRFPISAP
Sbjct: 181 HCPSCRFPISAP 192

BLAST of MS005230 vs. ExPASy TrEMBL
Match: A0A0A0LG47 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G009360 PE=4 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 8.2e-68
Identity = 143/200 (71.50%), Postives = 160/200 (80.00%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHL-TSSSTQQR 60
           MAGMLPGVECARRRRFHQSI SSDSP TA K GSTRRPSFCLYTSNRDFHL +SSS+QQR
Sbjct: 1   MAGMLPGVECARRRRFHQSINSSDSPFTAGKSGSTRRPSFCLYTSNRDFHLISSSSSQQR 60

Query: 61  SWSNQAFQNDKLGEIAREAKERLDERLKTRRKPETETVSKREEEVRSK-------GSEKE 120
           S +NQA+QNDKLGEIAREAKERLDERL+T RKPE        EEV+ K       G +KE
Sbjct: 61  SLTNQAYQNDKLGEIAREAKERLDERLRTHRKPENSRKKNNNEEVKGKESREKNNGEKKE 120

Query: 121 GEMTKGKEDERKKKLMSWG----KWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCL 180
            + TKGKE+E KK+  SWG    KWKA EQ+EC+VCLE FR  EP++HLPCAHKFHS+CL
Sbjct: 121 MKNTKGKEEETKKR-FSWGRKLMKWKASEQEECSVCLERFRLSEPLLHLPCAHKFHSTCL 180

Query: 181 VPWLQTNSHCPSCRFPISAP 189
           VPWLQ N+HCP CRFPI+ P
Sbjct: 181 VPWLQANAHCPCCRFPIAVP 199

BLAST of MS005230 vs. ExPASy TrEMBL
Match: A0A6J1HQI5 (probable E3 ubiquitin-protein ligase RHY1A isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465688 PE=4 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 2.6e-66
Identity = 138/190 (72.63%), Postives = 149/190 (78.42%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHLTSSSTQQRS 60
           MAGMLPGVECARRRRFHQSITSS+S STAAKLGSTRR SFCLYTSNRDFHLTSSS+QQR 
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSESASTAAKLGSTRRSSFCLYTSNRDFHLTSSSSQQRC 60

Query: 61  WSNQAFQNDKLGEIAREAKERLDERLKTRRKPETETVSKREEEVRSKGSEKEGEMTKGKE 120
            SN A+QN+KLG+ AREAKERLDERL+T RKPE        EEVR K      E  KGKE
Sbjct: 61  SSNHAYQNEKLGQAAREAKERLDERLRTHRKPENSRTKSSSEEVRLK-----KENVKGKE 120

Query: 121 DERKKKLMSWG---KWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNSH 180
           +   KK  SWG   KWKA EQ+EC VCLE FR  EP+VHLPCAHKFHS CLVPWLQ N+H
Sbjct: 121 ETTTKKRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQANAH 180

Query: 181 CPSCRFPISA 188
           CP CR+PI A
Sbjct: 181 CPCCRYPILA 185

BLAST of MS005230 vs. ExPASy TrEMBL
Match: A0A6J1G4D6 (probable E3 ubiquitin-protein ligase RHY1A OS=Cucurbita moschata OX=3662 GN=LOC111450678 PE=4 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 1.0e-65
Identity = 139/189 (73.54%), Postives = 153/189 (80.95%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHLTSSS--TQQ 60
           MAGMLPGVECARRRRFHQSITSSD+PSTAAKLGSTRRPSFCLYTSNRDFH+TSSS  +QQ
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCSQQ 60

Query: 61  RSWSNQAFQNDKLGEIAREAKERLDERLKTRRKPETETVSKREEEVRSKGSEKEGEMTKG 120
           RS S+QA+QN+KLGEIAREAKERLDERL+T RKPE                +K GE    
Sbjct: 61  RSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENP-------------RKKSGE--GW 120

Query: 121 KEDERKKKLMSWG---KWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTN 180
           KE+   KK  +WG   KWKA EQ+ECAVCLEGFR GEP+VHLPCAHKFHS+CLVPWLQTN
Sbjct: 121 KEESITKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTN 174

Query: 181 SHCPSCRFP 185
           +HCP CRFP
Sbjct: 181 AHCPCCRFP 174

BLAST of MS005230 vs. ExPASy TrEMBL
Match: A0A1S3C563 (RING-H2 finger protein ATL16 OS=Cucumis melo OX=3656 GN=LOC103496570 PE=4 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 5.5e-64
Identity = 137/195 (70.26%), Postives = 154/195 (78.97%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFHQSITSSDSPSTAAKLGSTRRPSFCLYTSNRDFHL-TSSSTQQR 60
           MAGMLPGVECARRRRFH SI SSDSPSTAAK GSTRRPSFCLYTSNRDFHL +SSS+QQR
Sbjct: 1   MAGMLPGVECARRRRFHHSINSSDSPSTAAKSGSTRRPSFCLYTSNRDFHLISSSSSQQR 60

Query: 61  SWSNQAFQNDKLGEIAREAKERLDERLKTRRKPE--------TETVSKREEEVRSKGSEK 120
           S +N+A QN+KLGEIAREAKERLDERL+T RKPE         E V  +E   ++ G  K
Sbjct: 61  SLTNKAHQNEKLGEIAREAKERLDERLRTHRKPENSRSKNSKNEEVKWKESREKNNGERK 120

Query: 121 EGEMTKGKEDERKKKLMSWG----KWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSC 180
           E   TKGKE+E +K+  SWG    KWKA EQ EC+VCLE FR GEP++HLPCAHKFHS+C
Sbjct: 121 EMRSTKGKEEETRKR-FSWGRKLMKWKASEQDECSVCLERFRSGEPLLHLPCAHKFHSTC 180

Query: 181 LVPWLQTNSHCPSCR 183
           LVPWLQ N+HCP CR
Sbjct: 181 LVPWLQANAHCPCCR 194

BLAST of MS005230 vs. TAIR 10
Match: AT2G15580.1 (RING/U-box superfamily protein )

HSP 1 Score: 144.1 bits (362), Expect = 1.2e-34
Identity = 94/196 (47.96%), Postives = 121/196 (61.73%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFH---QSITSSDSPSTAAKLGS--TRRPSFCLYTSNRDFHLTSSS 60
           MAGMLPGVECARRRRFH     I SS++ S AA  G   TRRPSF LYT+N + H    S
Sbjct: 1   MAGMLPGVECARRRRFHGGAPPIESSNTASVAAAAGHVWTRRPSFSLYTTNHESHQAHVS 60

Query: 61  TQQRSWSNQAFQND---KLGEIAREAKERLDERLKTRRKPETETVSKREEEVRSKGSEKE 120
             +RS  N+++  D   KL   A+EAK+RL++RL  R  P T    K +     +G  K 
Sbjct: 61  FSERSVRNKSYGEDNDEKLDGAAKEAKQRLNKRL--RIPPRTRQNGKDKGNKLEQGKGKP 120

Query: 121 -GEMTKGKEDERKK--KLMSWGKWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLV 180
            G++       +K   +LM W K +  EQ++CA+CL+ F+ GE +VHLPCAHKFHS CL+
Sbjct: 121 LGDLPTEVVGLKKSRGRLMEWFKRRVREQQDCAICLDRFKKGETLVHLPCAHKFHSICLL 180

Query: 181 PWLQTNSHCPSCRFPI 186
           PWL TN +CP CR  I
Sbjct: 181 PWLDTNVYCPYCRTDI 194

BLAST of MS005230 vs. TAIR 10
Match: AT2G15580.2 (RING/U-box superfamily protein )

HSP 1 Score: 72.0 bits (175), Expect = 5.7e-13
Identity = 54/123 (43.90%), Postives = 75/123 (60.98%), Query Frame = 0

Query: 1   MAGMLPGVECARRRRFH---QSITSSDSPSTAAKLGS--TRRPSFCLYTSNRDFHLTSSS 60
           MAGMLPGVECARRRRFH     I SS++ S AA  G   TRRPSF LYT+N + H    S
Sbjct: 1   MAGMLPGVECARRRRFHGGAPPIESSNTASVAAAAGHVWTRRPSFSLYTTNHESHQAHVS 60

Query: 61  TQQRSWSNQAFQND---KLGEIAREAKERLDERLK------TRRKPETETVSKREEEVRS 110
             +RS  N+++  D   KL   A+EAK+RL++RL+      + +  +T+ ++  +E V  
Sbjct: 61  FSERSVRNKSYGEDNDEKLDGAAKEAKQRLNKRLRIPPRTSSGKMVKTKGINWSKERVNL 120

BLAST of MS005230 vs. TAIR 10
Match: AT1G49850.1 (RING/U-box superfamily protein )

HSP 1 Score: 67.8 bits (164), Expect = 1.1e-11
Identity = 25/50 (50.00%), Postives = 34/50 (68.00%), Query Frame = 0

Query: 137 EQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNSHCPSCRFPIS 187
           E ++C++CLE F  G+ ++ LPC H FHSSCL PWL+    CP CR  I+
Sbjct: 199 EMRDCSICLESFTKGDMLISLPCTHSFHSSCLNPWLRACGDCPCCRRAIA 248

BLAST of MS005230 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 66.2 bits (160), Expect = 3.1e-11
Identity = 23/49 (46.94%), Postives = 32/49 (65.31%), Query Frame = 0

Query: 137 EQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNSHCPSCRFPI 186
           E  +CAVC++ F  G  V  +PC H FH  CL+PWL+ ++ CP CRF +
Sbjct: 212 EMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFEL 260

BLAST of MS005230 vs. TAIR 10
Match: AT3G58720.1 (RING/U-box superfamily protein )

HSP 1 Score: 65.9 bits (159), Expect = 4.1e-11
Identity = 31/71 (43.66%), Postives = 39/71 (54.93%), Query Frame = 0

Query: 118 GKEDERKKKLMSWGKWKAWEQKECAVCLEGFRGGEPVVHLPCAHKFHSSCLVPWLQTNSH 177
           G E  R  + +S  +    + K C+VCLE F   E V+  PC H FH  C+VPWL+T   
Sbjct: 125 GSESSRNTQNLSGEE----DDKRCSVCLEDFEPKETVMLTPCKHMFHEECIVPWLKTKGQ 184

Query: 178 CPSCRFPISAP 189
           CP CRF I  P
Sbjct: 185 CPVCRFVILKP 191

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022143236.14.1e-9896.88E3 ubiquitin-protein ligase EL5 [Momordica charantia][more]
XP_004139268.11.7e-6771.50probable E3 ubiquitin-protein ligase RHY1A [Cucumis sativus] >KGN60748.1 hypothe... [more]
KAG7030344.13.8e-6774.07putative E3 ubiquitin-protein ligase RHY1A, partial [Cucurbita argyrosperma subs... [more]
XP_022965966.15.4e-6672.63probable E3 ubiquitin-protein ligase RHY1A isoform X1 [Cucurbita maxima] >XP_022... [more]
XP_038891102.19.3e-6674.36probable E3 ubiquitin-protein ligase RHY1A [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q852U61.5e-1050.00Probable E3 ubiquitin-protein ligase RHY1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q8LPN74.4e-1046.94E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
Q9C1X42.2e-0944.90Uncharacterized RING finger protein P32A8.03c OS=Schizosaccharomyces pombe (stra... [more]
Q6AVN26.3e-0939.71E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI... [more]
Q91YL21.1e-0855.81E3 ubiquitin-protein ligase RNF126 OS=Mus musculus OX=10090 GN=Rnf126 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CPN12.0e-9896.88E3 ubiquitin-protein ligase EL5 OS=Momordica charantia OX=3673 GN=LOC111013152 P... [more]
A0A0A0LG478.2e-6871.50RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G009360 P... [more]
A0A6J1HQI52.6e-6672.63probable E3 ubiquitin-protein ligase RHY1A isoform X1 OS=Cucurbita maxima OX=366... [more]
A0A6J1G4D61.0e-6573.54probable E3 ubiquitin-protein ligase RHY1A OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A1S3C5635.5e-6470.26RING-H2 finger protein ATL16 OS=Cucumis melo OX=3656 GN=LOC103496570 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G15580.11.2e-3447.96RING/U-box superfamily protein [more]
AT2G15580.25.7e-1343.90RING/U-box superfamily protein [more]
AT1G49850.11.1e-1150.00RING/U-box superfamily protein [more]
AT3G19950.13.1e-1146.94RING/U-box superfamily protein [more]
AT3G58720.14.1e-1143.66RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 141..181
e-value: 1.5E-6
score: 37.8
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 139..182
e-value: 8.0E-13
score: 48.5
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 141..182
score: 12.504031
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 114..187
e-value: 1.9E-18
score: 67.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 20..36
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 82..123
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 16..36
NoneNo IPR availablePANTHERPTHR22765:SF204RING/U-BOX SUPERFAMILY PROTEINcoord: 125..184
NoneNo IPR availablePANTHERPTHR22765RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAININGcoord: 1..102
coord: 125..184
NoneNo IPR availablePANTHERPTHR22765:SF204RING/U-BOX SUPERFAMILY PROTEINcoord: 1..102
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 140..182
e-value: 2.42689E-18
score: 72.7197
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 129..187

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS005230.1MS005230.1mRNA