MS005133 (gene) Bitter gourd (TR) v1

Overview
NameMS005133
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionPhospholipid:diacylglycerol acyltransferase
Locationscaffold176: 2924515 .. 2928114 (-)
RNA-Seq ExpressionMS005133
SyntenyMS005133
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCGTTCTTCGCCTCCGAAAATTGTACTATGTCGACCCTGTTAAGAGCTCTTCCATGGGGATCAACCCCAACGAGAAATCTGAGGATAAACGCAATGTTATTGTTGCATACGTGAAGAAATTGCTGGAGAAGAAGCAAGAGAAGGACAAGAGGAAGAAAAAGAAGAACATCAAGAACAAGGGGGGAGGCAAAGAATGGAAGTGCTTAGATAACTGTTGTTGGATTCTTGGATATATGTGCACAATTTGGTGGGTGCTCTTTTTTCTCTACCATTACTTACCACCTGGGTTTCCGGTGATTGATTCTCCGGGGAGCGTTCTAAAGCGTGAAGGCGTTTCAGCTCTTCACCCGGTGGTTTTAGTGCCCGGACTTGTCACCGGCGGGCTGGAGCTCTGGGAAGGCAAGCCCTGCGCCAAGGGCCTCTTTCGTAAGCGCCTGTGGGGTGGGAGTTTCTCTGAAACATTGAAAAGGTTAGAAAATTTCAAAGCAAATTTACATTCTAAAGTACACATTTTGAGAACTGAATTGAAAATTTGCAGACCTTTGTGCTGGCTGGAGCACCTGTCTCTGGACAATGAAACAGGGCTTGACCCACCAGGCATTCGAGTCCGGCCAGTCGAAGGGCTTGTCGCCGGTGATTACTTCGCCCAAGGCTACTTTGTTTGGGCTGTTCTCATTGAAAATCTGGCTAAAATTGGCTACGATGGAAAGAACCTTCACATGGCTGCATATGATTGGAGGATCGCCTTCCAAAACACAGAGGTATGCCACCCAAAACCCATCTTCTTCCAATCAAAATAGATATTCACTTCACTGGGTTTGCCATTGTTGAGAGTAAACGCAGGTTCGAGACAGAGCTCTGAGCAGATTGAAGAGTAAAATAGAGGTAATGTATGAAACAAATGGGGGAGAGAAAGTGGTGGTGGTGCCTCATTCAATGGGGGTTCTGTACTTCCTCTACTTCATGAAGTGGGTGGAAGCGCCGCCGGCGATAGGCGGCGGCGGGGGCGGCCAAGGGTGGTGCGGCAAGCATATTAAAGCAATCATGAATATTGGGTCTACATTTCTGGGAACTCCAAAGGCTGTTAGTAAAATTATTTCTGGTGAAGACAGATCAATTGCTTCGCTCAGGTTCTCTTCTCTGCACTCTAAAGTCTAAGTTATAATTTTTATTCAAATAATAAGTATACTGTAGTAATTACTTTAGTTAGATTATTTTACTTGTATTCAATGCATATATTATGTCGATTTTGTTATCAGTTCTTTCTTATTTTGATTTTCCTTTTTCATAGATGTTTTGACAAAGTCCCCAATCTTCATTTCTTTTTTTCTTTTTTCTATTTCCTTTTTAGTAAGATTTAAAGTATTTGGGATAAAATGTTTAATTTTTTTTATAAGTTAAAAGTTTTGGACATTGTAGTTAACTTATATTTATTTATTACTTAAATTACATATTGAAAATCATGAAATTGAAAACTACACCAAAGCTTAAAAAGATATTTATTCTAAACTAGTACAGCTTTTGAAAATATATAATAAATATGTAATGGAATTCTTTTTCTTTTCTATTTCATAAGTTAAACAGAACAAGAAATAGTAAACTTCTAAAGCTGTGTCAAAATCGTGACTCAAGTGTAAGGTTTACTCGAAAATTATTTTTAAAAGATACTTTCATGACTAAAAAGTAGTTGATGTTTTGTGATTTTCCCTTTTTATTTGGACAAAAAGCACAAAATGATCGTCAATGATCAAACATTATTCTCTAAGAGCAACTAAGAAGCTAAAAAAACTCGTTTCTTATTCTTCTTTGGAAACAAACAAAAAAAGTAGAAACGAGTTTAATTGATGTTTTCTTTTTTATATTCCTCAAATTTATCTATTCAAGATGGGATACACCTGCAGATGCCAGGCAAAGAGAAGCCTGCTTATAGAGATTCAAGGACAAATTATTGAAATTAGCTTTCTTGGCATTTTACAGAGCTATGACCCCTGGTTTTTTGAAATCTGAAATTCTTGGCTTTCAAACCTTAGAACATATGTTGCGGGCTTCCCGAACGTGGGACTCAACCGCCTCATTGTTACCAAAAGGTGGGGAGACCTTCTGGGGTGACTTAGATTGGTCTCCCGAAGATGTTCAGAATTGTGATTTGGAGAAAGTAGATACACATTCTTTTAGAAACGAAACTGATTCCAATGACAGTGAGAGACAGAAAAGGGAAGGTTTTCAACAAAAGGAGGCAGTGCACTACGGAAGAATAATTTCTTTTAGCAGAGAAGCAGCAACACGGCCTTCTTCAGAGCTCTCTACTCTAACTTCACAGGTAATCCTTTGGCTAGTTTCTGTGCATTGGAACTAACTTCTTTGATGGGGCAGATGATGAAAACTTAGCTCTTTCAACACATCCATTTAAAGAATAAAGCCAACAGAGAAATTCCACATAACTTGAATAGCTTGCTTAATGCATTAGTCACTATTTTTGTTGATAGGCGTGCTTATATATTCGAAGAGGGAATATTTTTCTATTCATCTTGAACAGTAATGACTAATGTGTGGATCTTGAATAGGAAATTTTGGATTCGGGTAGCTCCAATGATTCGAGCTTCTCTTGTAGCGACGTATGGACTGAATACGATGAGATGAGTAGGGAAAGTATTCAAAAAGTCTCTGAGAACAAAGCTTACACAGCAAAAACTGTGTTCGATCTACTCCGAGTCGTGGCTCCAAGAATGATGCAACGGGTCGAGAGTCACTTCTCTCATGGGATTGCTGAAGATCTTGATGATCCTAAATATGCTCATGATCACAAGTACTGGTCAAACCCACTTGAGACAAAGTAAGTACCTAGTGTTTATATTTAAGAAATTTCTGATTTTCTTTAGAAGATTTTCTTCCTATGAGGTATTGCATCCATTCAAGTCTTGGTAGGAGTTGATAATCCAATTAGCAATCTAACAACATCAAGAACAAGGTTCTGGAGGAATAATTCCCTTCATCCTCTCCCTCAATCTCTGACCCCCAAGGAAAGGAAGACGTCTTTCTTAACTTTCATTTTCATTTCTGTCCTGCTGCCACAATTCATGGAACTTGACATTTTTTTTTGGCAAATCTGTTTCAGACTACCAGATGCCCCAGATATGGAGATATACTGCTTATACGGAGTGGGAATCCCCACAGAAAGATCATACATCTATGACTCATCTTCTGACAAGTGTAGGAGACTTCCATTCAGGATTGATCTCTCAGTCGATGGAAAAGAGAATGGTTGTCTCAGAAGTGGTATGTATTTCGTGGATGGCGACGACTGTGTACCGGTCTTGAGCTCGGGTTTCATGTGTGCTAAAGGATGGCGAAACACAACACGATTCAACCCATCGGGCAGCCCGACGTACGTAAGAGAATACATGCACAAGGCTCCTGCTAGTCTGCTAGAAGGAAGAGGAGTTGAAAGTAGTGCTCATGTTGATATAATGGGAAATGTTAAACTAATTGAAGATATCTTGAGGGTGGCTGCTGGAGCAAGTGGGACAGAAATTGGTGGTGATAGGATTTACTCTGATATCTTAACACTATCAGAGAGAATAAATATTAAGCTG

mRNA sequence

ATGGCTTCCGTTCTTCGCCTCCGAAAATTGTACTATGTCGACCCTGTTAAGAGCTCTTCCATGGGGATCAACCCCAACGAGAAATCTGAGGATAAACGCAATGTTATTGTTGCATACGTGAAGAAATTGCTGGAGAAGAAGCAAGAGAAGGACAAGAGGAAGAAAAAGAAGAACATCAAGAACAAGGGGGGAGGCAAAGAATGGAAGTGCTTAGATAACTGTTGTTGGATTCTTGGATATATGTGCACAATTTGGTGGGTGCTCTTTTTTCTCTACCATTACTTACCACCTGGGTTTCCGGTGATTGATTCTCCGGGGAGCGTTCTAAAGCGTGAAGGCGTTTCAGCTCTTCACCCGGTGGTTTTAGTGCCCGGACTTGTCACCGGCGGGCTGGAGCTCTGGGAAGGCAAGCCCTGCGCCAAGGGCCTCTTTCGTAAGCGCCTGTGGGGTGGGAGTTTCTCTGAAACATTGAAAAGGTTAGAAAATTTCAAAGCAAATTTACATTCTAAAGTACACATTTTGAGAACTGAATTGAAAATTTGCAGACCTTTGTGCTGGCTGGAGCACCTGTCTCTGGACAATGAAACAGGGCTTGACCCACCAGGCATTCGAGTCCGGCCAGTCGAAGGGCTTGTCGCCGGTGATTACTTCGCCCAAGGCTACTTTGTTTGGGCTGTTCTCATTGAAAATCTGGCTAAAATTGGCTACGATGGAAAGAACCTTCACATGGCTGCATATGATTGGAGGATCGCCTTCCAAAACACAGAGGTTCGAGACAGAGCTCTGAGCAGATTGAAGAGTAAAATAGAGGTAATGTATGAAACAAATGGGGGAGAGAAAGTGGTGGTGGTGCCTCATTCAATGGGGGTTCTGTACTTCCTCTACTTCATGAAGTGGGTGGAAGCGCCGCCGGCGATAGGCGGCGGCGGGGGCGGCCAAGGGTGGTGCGGCAAGCATATTAAAGCAATCATGAATATTGGGTCTACATTTCTGGGAACTCCAAAGGCTGTTAGTAAAATTATTTCTGGTGAAGACAGATCAATTGCTTCGCTCAGAGCTATGACCCCTGGTTTTTTGAAATCTGAAATTCTTGGCTTTCAAACCTTAGAACATATGTTGCGGGCTTCCCGAACGTGGGACTCAACCGCCTCATTGTTACCAAAAGGTGGGGAGACCTTCTGGGGTGACTTAGATTGGTCTCCCGAAGATGTTCAGAATTGTGATTTGGAGAAAGTAGATACACATTCTTTTAGAAACGAAACTGATTCCAATGACAGTGAGAGACAGAAAAGGGAAGGTTTTCAACAAAAGGAGGCAGTGCACTACGGAAGAATAATTTCTTTTAGCAGAGAAGCAGCAACACGGCCTTCTTCAGAGCTCTCTACTCTAACTTCACAGGAAATTTTGGATTCGGGTAGCTCCAATGATTCGAGCTTCTCTTGTAGCGACGTATGGACTGAATACGATGAGATGAGTAGGGAAAGTATTCAAAAAGTCTCTGAGAACAAAGCTTACACAGCAAAAACTGTGTTCGATCTACTCCGAGTCGTGGCTCCAAGAATGATGCAACGGGTCGAGAGTCACTTCTCTCATGGGATTGCTGAAGATCTTGATGATCCTAAATATGCTCATGATCACAAGTACTGGTCAAACCCACTTGAGACAAAACTACCAGATGCCCCAGATATGGAGATATACTGCTTATACGGAGTGGGAATCCCCACAGAAAGATCATACATCTATGACTCATCTTCTGACAAGTGTAGGAGACTTCCATTCAGGATTGATCTCTCAGTCGATGGAAAAGAGAATGGTTGTCTCAGAAGTGGTATGTATTTCGTGGATGGCGACGACTGTGTACCGGTCTTGAGCTCGGGTTTCATGTGTGCTAAAGGATGGCGAAACACAACACGATTCAACCCATCGGGCAGCCCGACGTACGTAAGAGAATACATGCACAAGGCTCCTGCTAGTCTGCTAGAAGGAAGAGGAGTTGAAAGTAGTGCTCATGTTGATATAATGGGAAATGTTAAACTAATTGAAGATATCTTGAGGGTGGCTGCTGGAGCAAGTGGGACAGAAATTGGTGGTGATAGGATTTACTCTGATATCTTAACACTATCAGAGAGAATAAATATTAAGCTG

Coding sequence (CDS)

ATGGCTTCCGTTCTTCGCCTCCGAAAATTGTACTATGTCGACCCTGTTAAGAGCTCTTCCATGGGGATCAACCCCAACGAGAAATCTGAGGATAAACGCAATGTTATTGTTGCATACGTGAAGAAATTGCTGGAGAAGAAGCAAGAGAAGGACAAGAGGAAGAAAAAGAAGAACATCAAGAACAAGGGGGGAGGCAAAGAATGGAAGTGCTTAGATAACTGTTGTTGGATTCTTGGATATATGTGCACAATTTGGTGGGTGCTCTTTTTTCTCTACCATTACTTACCACCTGGGTTTCCGGTGATTGATTCTCCGGGGAGCGTTCTAAAGCGTGAAGGCGTTTCAGCTCTTCACCCGGTGGTTTTAGTGCCCGGACTTGTCACCGGCGGGCTGGAGCTCTGGGAAGGCAAGCCCTGCGCCAAGGGCCTCTTTCGTAAGCGCCTGTGGGGTGGGAGTTTCTCTGAAACATTGAAAAGGTTAGAAAATTTCAAAGCAAATTTACATTCTAAAGTACACATTTTGAGAACTGAATTGAAAATTTGCAGACCTTTGTGCTGGCTGGAGCACCTGTCTCTGGACAATGAAACAGGGCTTGACCCACCAGGCATTCGAGTCCGGCCAGTCGAAGGGCTTGTCGCCGGTGATTACTTCGCCCAAGGCTACTTTGTTTGGGCTGTTCTCATTGAAAATCTGGCTAAAATTGGCTACGATGGAAAGAACCTTCACATGGCTGCATATGATTGGAGGATCGCCTTCCAAAACACAGAGGTTCGAGACAGAGCTCTGAGCAGATTGAAGAGTAAAATAGAGGTAATGTATGAAACAAATGGGGGAGAGAAAGTGGTGGTGGTGCCTCATTCAATGGGGGTTCTGTACTTCCTCTACTTCATGAAGTGGGTGGAAGCGCCGCCGGCGATAGGCGGCGGCGGGGGCGGCCAAGGGTGGTGCGGCAAGCATATTAAAGCAATCATGAATATTGGGTCTACATTTCTGGGAACTCCAAAGGCTGTTAGTAAAATTATTTCTGGTGAAGACAGATCAATTGCTTCGCTCAGAGCTATGACCCCTGGTTTTTTGAAATCTGAAATTCTTGGCTTTCAAACCTTAGAACATATGTTGCGGGCTTCCCGAACGTGGGACTCAACCGCCTCATTGTTACCAAAAGGTGGGGAGACCTTCTGGGGTGACTTAGATTGGTCTCCCGAAGATGTTCAGAATTGTGATTTGGAGAAAGTAGATACACATTCTTTTAGAAACGAAACTGATTCCAATGACAGTGAGAGACAGAAAAGGGAAGGTTTTCAACAAAAGGAGGCAGTGCACTACGGAAGAATAATTTCTTTTAGCAGAGAAGCAGCAACACGGCCTTCTTCAGAGCTCTCTACTCTAACTTCACAGGAAATTTTGGATTCGGGTAGCTCCAATGATTCGAGCTTCTCTTGTAGCGACGTATGGACTGAATACGATGAGATGAGTAGGGAAAGTATTCAAAAAGTCTCTGAGAACAAAGCTTACACAGCAAAAACTGTGTTCGATCTACTCCGAGTCGTGGCTCCAAGAATGATGCAACGGGTCGAGAGTCACTTCTCTCATGGGATTGCTGAAGATCTTGATGATCCTAAATATGCTCATGATCACAAGTACTGGTCAAACCCACTTGAGACAAAACTACCAGATGCCCCAGATATGGAGATATACTGCTTATACGGAGTGGGAATCCCCACAGAAAGATCATACATCTATGACTCATCTTCTGACAAGTGTAGGAGACTTCCATTCAGGATTGATCTCTCAGTCGATGGAAAAGAGAATGGTTGTCTCAGAAGTGGTATGTATTTCGTGGATGGCGACGACTGTGTACCGGTCTTGAGCTCGGGTTTCATGTGTGCTAAAGGATGGCGAAACACAACACGATTCAACCCATCGGGCAGCCCGACGTACGTAAGAGAATACATGCACAAGGCTCCTGCTAGTCTGCTAGAAGGAAGAGGAGTTGAAAGTAGTGCTCATGTTGATATAATGGGAAATGTTAAACTAATTGAAGATATCTTGAGGGTGGCTGCTGGAGCAAGTGGGACAGAAATTGGTGGTGATAGGATTTACTCTGATATCTTAACACTATCAGAGAGAATAAATATTAAGCTG

Protein sequence

MASVLRLRKLYYVDPVKSSSMGINPNEKSEDKRNVIVAYVKKLLEKKQEKDKRKKKKNIKNKGGGKEWKCLDNCCWILGYMCTIWWVLFFLYHYLPPGFPVIDSPGSVLKREGVSALHPVVLVPGLVTGGLELWEGKPCAKGLFRKRLWGGSFSETLKRLENFKANLHSKVHILRTELKICRPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAGDYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGEKVVVVPHSMGVLYFLYFMKWVEAPPAIGGGGGGQGWCGKHIKAIMNIGSTFLGTPKAVSKIISGEDRSIASLRAMTPGFLKSEILGFQTLEHMLRASRTWDSTASLLPKGGETFWGDLDWSPEDVQNCDLEKVDTHSFRNETDSNDSERQKREGFQQKEAVHYGRIISFSREAATRPSSELSTLTSQEILDSGSSNDSSFSCSDVWTEYDEMSRESIQKVSENKAYTAKTVFDLLRVVAPRMMQRVESHFSHGIAEDLDDPKYAHDHKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSSDKCRRLPFRIDLSVDGKENGCLRSGMYFVDGDDCVPVLSSGFMCAKGWRNTTRFNPSGSPTYVREYMHKAPASLLEGRGVESSAHVDIMGNVKLIEDILRVAAGASGTEIGGDRIYSDILTLSERINIKL
Homology
BLAST of MS005133 vs. NCBI nr
Match: XP_022140787.1 (putative phospholipid:diacylglycerol acyltransferase 2 [Momordica charantia])

HSP 1 Score: 1402.9 bits (3630), Expect = 0.0e+00
Identity = 691/715 (96.64%), Postives = 692/715 (96.78%), Query Frame = 0

Query: 1   MASVLRLRKLYYVDPVKSSSMGINPNEKSEDKRNVIVAYVKKLLEKKQEKDKRKKKKNIK 60
           MASVLRLRKLYYVDPVKSSSMGINPNEKSEDKRNVIVAYVKKLLEKKQEKDKRKKKKNIK
Sbjct: 1   MASVLRLRKLYYVDPVKSSSMGINPNEKSEDKRNVIVAYVKKLLEKKQEKDKRKKKKNIK 60

Query: 61  NKGGGKEWKCLDNCCWILGYMCTIWWVLFFLYHYLPPGFPVIDSPGSVLKREGVSALHPV 120
           NKGGGKEWKCLDNCCWILGYMCTIWWVLFFLYHYLPPGFPVIDSPGSVLKREGVSALHPV
Sbjct: 61  NKGGGKEWKCLDNCCWILGYMCTIWWVLFFLYHYLPPGFPVIDSPGSVLKREGVSALHPV 120

Query: 121 VLVPGLVTGGLELWEGKPCAKGLFRKRLWGGSFSETLKRLENFKANLHSKVHILRTELKI 180
           VLVPGLVTGGLELWEGKPCAKGLFRKRLWGGSFSETLK                      
Sbjct: 121 VLVPGLVTGGLELWEGKPCAKGLFRKRLWGGSFSETLK---------------------- 180

Query: 181 CRPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAGDYFAQGYFVWAVLIENLAKIGYDGKN 240
            RPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAGDYFAQGYFVWAVLIENLAKIGYDGKN
Sbjct: 181 -RPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAGDYFAQGYFVWAVLIENLAKIGYDGKN 240

Query: 241 LHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGEKVVVVPHSMGVLYFLYFMKWV 300
           LHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGEKVVVVPHSMGVLYFLYFMKWV
Sbjct: 241 LHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGEKVVVVPHSMGVLYFLYFMKWV 300

Query: 301 EAPPAIGGGGGGQGWCGKHIKAIMNIGSTFLGTPKAVSKIISGEDRSIASLRAMTPGFLK 360
           EAPPAIGGGGGGQGWCGKHIKAIMNIGSTFLGTPKAVSKIISGEDRSIASLRAMTPGFLK
Sbjct: 301 EAPPAIGGGGGGQGWCGKHIKAIMNIGSTFLGTPKAVSKIISGEDRSIASLRAMTPGFLK 360

Query: 361 SEILGFQTLEHMLRASRTWDSTASLLPKGGETFWGDLDWSPEDVQNCDLEKVDTHSFRNE 420
           SEILGFQTLEHMLRASRTWDSTASLLPKGGETFWGDLDWSPEDVQNCDLEKVDTHSFRNE
Sbjct: 361 SEILGFQTLEHMLRASRTWDSTASLLPKGGETFWGDLDWSPEDVQNCDLEKVDTHSFRNE 420

Query: 421 TDSNDSERQKREGFQQKEAVHYGRIISFSREAATRPSSELSTLTSQEILDSGSSNDSSFS 480
           TDSNDSERQKREGFQQKEAVHYGRIISFSREAATRPSSELSTLTSQEILDSGSSNDSSFS
Sbjct: 421 TDSNDSERQKREGFQQKEAVHYGRIISFSREAATRPSSELSTLTSQEILDSGSSNDSSFS 480

Query: 481 CSDVWTEYDEMSRESIQKVSENKAYTAKTVFDLLRVVAPRMMQRVESHFSHGIAEDLDDP 540
           CSDVWTEYDEMSRESIQKVSENKAYTAKTVFDLLRVVAPRMMQRVESHFSHGIAEDLDDP
Sbjct: 481 CSDVWTEYDEMSRESIQKVSENKAYTAKTVFDLLRVVAPRMMQRVESHFSHGIAEDLDDP 540

Query: 541 KYAHDHKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSSDKCRRLPFRIDLSVD 600
           KYAHDHKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSSDKCRRLPFRIDLSVD
Sbjct: 541 KYAHDHKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSSDKCRRLPFRIDLSVD 600

Query: 601 GKENGCLRSGMYFVDGDDCVPVLSSGFMCAKGWRNTTRFNPSGSPTYVREYMHKAPASLL 660
           GKENGCLRSGMYFVDGDDCVPVLSSGFMCAKGWRNTTRFNPSGSPTYVREYMHKAPASLL
Sbjct: 601 GKENGCLRSGMYFVDGDDCVPVLSSGFMCAKGWRNTTRFNPSGSPTYVREYMHKAPASLL 660

Query: 661 EGRGVESSAHVDIMGNVKLIEDILRVAAGASGTEIGGDRIYSDILTLSERINIKL 716
           EGRGVESSAHVDIMGNVKLIEDILRVAAGASGTE+GGDRIYSDILTLSERINIKL
Sbjct: 661 EGRGVESSAHVDIMGNVKLIEDILRVAAGASGTEVGGDRIYSDILTLSERINIKL 692

BLAST of MS005133 vs. NCBI nr
Match: XP_022986276.1 (putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita maxima])

HSP 1 Score: 1233.8 bits (3191), Expect = 0.0e+00
Identity = 605/721 (83.91%), Postives = 651/721 (90.29%), Query Frame = 0

Query: 1   MASVLRLRKLYYVDPVKSSSMG---INPNEKSEDKRNVIVAYVKKLLEKKQEKDKRKKKK 60
           MASVLRLRKLYYV+PVK+SSMG   I+   KSEDK NV+VAYVKK+L+KKQ+KD++KK+ 
Sbjct: 1   MASVLRLRKLYYVNPVKNSSMGIQTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRN 60

Query: 61  NIKNKGGGKEWKCLDNCCWILGYMCTIWWVLFFLYHYLP---PGFPVIDSPGSVLKREGV 120
              NKG  +EWKCLDNCCWI+GYMCT WW++FF+YHYLP   PGFP IDSPG+VLKR+GV
Sbjct: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120

Query: 121 SALHPVVLVPGLVTGGLELWEGKPCAKGLFRKRLWGGSFSETLKRLENFKANLHSKVHIL 180
           S LHPVVLVPG+VTGGLELW+GKPCAKGLFRKRLWGGSF+ETLK                
Sbjct: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLK---------------- 180

Query: 181 RTELKICRPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAGDYFAQGYFVWAVLIENLAKI 240
                  RPLCWLEHLSLDNETGLDPPGIR+RPVEGLVA DYF QGYFVWAVLIENLAKI
Sbjct: 181 -------RPLCWLEHLSLDNETGLDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKI 240

Query: 241 GYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGEKVVVVPHSMGVLYFL 300
           GYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGG KVVVVPHSMGVLYFL
Sbjct: 241 GYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFL 300

Query: 301 YFMKWVEAPPAIGGGGGGQGWCGKHIKAIMNIGSTFLGTPKAVSKIISGEDRSIASLRAM 360
           +FMKWVEAPP + GGGGG GWC K+IKAIMNIGSTFLGTPKAVSKI+S EDR IA LRAM
Sbjct: 301 HFMKWVEAPPPM-GGGGGLGWCAKYIKAIMNIGSTFLGTPKAVSKILSAEDRHIALLRAM 360

Query: 361 TPGFLKSEILGFQTLEHMLRASRTWDSTASLLPKGGETFWGDLDWSPEDVQNCDLEKVDT 420
           TPGFLKSEILGFQTLEHMLR SRTWDSTASLLPKGGET WGDLDWSPEDVQNCDLEKVDT
Sbjct: 361 TPGFLKSEILGFQTLEHMLRVSRTWDSTASLLPKGGETIWGDLDWSPEDVQNCDLEKVDT 420

Query: 421 HSFRNETDSNDSERQKREGFQQKEAVHYGRIISFSREAATRPSSELSTLTSQEILDSGSS 480
           HSFRNE DSNDSE +K  G Q+KEAVHYGR+ISF +EAATRPSSELSTL S+E++DSGSS
Sbjct: 421 HSFRNENDSNDSEIKK--GLQEKEAVHYGRMISFGKEAATRPSSELSTLYSEELMDSGSS 480

Query: 481 NDSSFSCSDVWTEYDEMSRESIQKVSENKAYTAKTVFDLLRVVAPRMMQRVESHFSHGIA 540
           NDSSFSCSD WTEYDEMSRES++KVSENKAYTAKTVFDLLRVVAP+MMQR++SHFSHGIA
Sbjct: 481 NDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSHFSHGIA 540

Query: 541 EDLDDPKYAHDHKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSSDKCRRLPFR 600
           EDLDDPKYAH +KYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSS DKC+ +PFR
Sbjct: 541 EDLDDPKYAH-YKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFR 600

Query: 601 IDLSVDGKENGCLRSGMYFVDGDDCVPVLSSGFMCAKGWRNTTRFNPSGSPTYVREYMHK 660
           IDLSVDG+ +GCLRSGMYFVDGDDCVPV+SSGFMCAKGWRN TRFNPSGSPTYVREY HK
Sbjct: 601 IDLSVDGEGSGCLRSGMYFVDGDDCVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHK 660

Query: 661 APASLLEGRGVESSAHVDIMGNVKLIEDILRVAAGASGTEIGGDRIYSDILTLSERINIK 716
           APASLLEGRG+ESSAHVDIMGN KLIEDILRVAAGA+G EIGGDRIYSDILTLSERIN+K
Sbjct: 661 APASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSERINLK 694

BLAST of MS005133 vs. NCBI nr
Match: XP_022944139.1 (putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita moschata])

HSP 1 Score: 1230.3 bits (3182), Expect = 0.0e+00
Identity = 601/721 (83.36%), Postives = 650/721 (90.15%), Query Frame = 0

Query: 1   MASVLRLRKLYYVDPVKSSSMG---INPNEKSEDKRNVIVAYVKKLLEKKQEKDKRKKKK 60
           MASVLRLRKLYYV+PVK+SSMG   I+   KSEDK NV+VAYVKK+L+KKQ+KD++KK+ 
Sbjct: 1   MASVLRLRKLYYVNPVKNSSMGIQTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRN 60

Query: 61  NIKNKGGGKEWKCLDNCCWILGYMCTIWWVLFFLYHYLP---PGFPVIDSPGSVLKREGV 120
              NKG  +EWKCLDNCCWI+GYMCT WW++FF+YHYLP   PGFP IDSPG+VLKR+GV
Sbjct: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120

Query: 121 SALHPVVLVPGLVTGGLELWEGKPCAKGLFRKRLWGGSFSETLKRLENFKANLHSKVHIL 180
           S LHPVVLVPG+VTGGLELW+GKPCAKGLFRKRLWGGSF+ETLK                
Sbjct: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLK---------------- 180

Query: 181 RTELKICRPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAGDYFAQGYFVWAVLIENLAKI 240
                  RPLCWLEHLSLDNETGLDPPGIR+RPVEGLVA DYF QGYFVWAVLIENLAKI
Sbjct: 181 -------RPLCWLEHLSLDNETGLDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKI 240

Query: 241 GYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGEKVVVVPHSMGVLYFL 300
           GYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGG +VVVVPHSMGVLYFL
Sbjct: 241 GYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGNRVVVVPHSMGVLYFL 300

Query: 301 YFMKWVEAPPAIGGGGGGQGWCGKHIKAIMNIGSTFLGTPKAVSKIISGEDRSIASLRAM 360
           +FMKWVEAPP + GGGGG GWC K+IKAIMNIGSTFLGTPKAVSKI+S EDR IA LRAM
Sbjct: 301 HFMKWVEAPPPM-GGGGGLGWCAKYIKAIMNIGSTFLGTPKAVSKILSAEDRHIALLRAM 360

Query: 361 TPGFLKSEILGFQTLEHMLRASRTWDSTASLLPKGGETFWGDLDWSPEDVQNCDLEKVDT 420
           TPGFLKSEILGFQTLEHMLR SRTWDSTASLLPKGGET WGDLDWSPEDVQNCDLEK DT
Sbjct: 361 TPGFLKSEILGFQTLEHMLRVSRTWDSTASLLPKGGETIWGDLDWSPEDVQNCDLEKADT 420

Query: 421 HSFRNETDSNDSERQKREGFQQKEAVHYGRIISFSREAATRPSSELSTLTSQEILDSGSS 480
           HSFRNE DSNDSE +K  GFQ+KEAVHYGR+ISF +EAATRPSSELSTL S+E++DSGSS
Sbjct: 421 HSFRNENDSNDSEIKK--GFQEKEAVHYGRMISFGKEAATRPSSELSTLYSEELMDSGSS 480

Query: 481 NDSSFSCSDVWTEYDEMSRESIQKVSENKAYTAKTVFDLLRVVAPRMMQRVESHFSHGIA 540
           ND+SFSCSD WTEYDEMSRES++KVSENKAYTAKTVFDLLRVVAP+MMQR++SHFSHGIA
Sbjct: 481 NDTSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSHFSHGIA 540

Query: 541 EDLDDPKYAHDHKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSSDKCRRLPFR 600
           EDLDDPKY H +KYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSS DKC+ +PFR
Sbjct: 541 EDLDDPKYGH-YKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFR 600

Query: 601 IDLSVDGKENGCLRSGMYFVDGDDCVPVLSSGFMCAKGWRNTTRFNPSGSPTYVREYMHK 660
           IDLS+DG+ +GCLRSGMYFVDGDDCVPV+SSGFMCAKGWRN TRFNPSGSPTYVREY HK
Sbjct: 601 IDLSIDGEGSGCLRSGMYFVDGDDCVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHK 660

Query: 661 APASLLEGRGVESSAHVDIMGNVKLIEDILRVAAGASGTEIGGDRIYSDILTLSERINIK 716
           APASLLEGRG+ESSAHVDIMGN KLIEDILRVAAGA+G EIGGDRIYSDILTLSERIN+K
Sbjct: 661 APASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSERINLK 694

BLAST of MS005133 vs. NCBI nr
Match: KAG6570836.1 (putative phospholipid:diacylglycerol acyltransferase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1230.3 bits (3182), Expect = 0.0e+00
Identity = 603/721 (83.63%), Postives = 650/721 (90.15%), Query Frame = 0

Query: 1   MASVLRLRKLYYVDPVKSSSMG---INPNEKSEDKRNVIVAYVKKLLEKKQEKDKRKKKK 60
           MASVLRLRKLYYV+PVK+SSMG   I+   KSEDK NV+VAYVKK+L+KKQ+KD++KK+ 
Sbjct: 1   MASVLRLRKLYYVNPVKNSSMGIRTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRS 60

Query: 61  NIKNKGGGKEWKCLDNCCWILGYMCTIWWVLFFLYHYLP---PGFPVIDSPGSVLKREGV 120
              NKG  +EWKCLDNCCWI+GYMCT WW++FF+YHYLP   PGFP IDSPG+VLKR+GV
Sbjct: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120

Query: 121 SALHPVVLVPGLVTGGLELWEGKPCAKGLFRKRLWGGSFSETLKRLENFKANLHSKVHIL 180
           S LHPVVLVPG+VTGGLELW+GKPCAKGLFRKRLWGGSF+ETLK                
Sbjct: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLK---------------- 180

Query: 181 RTELKICRPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAGDYFAQGYFVWAVLIENLAKI 240
                  RPLCWLEHLSLDNETGLDPPGIR+RPVEGLVA DYF QGYFVWAVLIENLAKI
Sbjct: 181 -------RPLCWLEHLSLDNETGLDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKI 240

Query: 241 GYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGEKVVVVPHSMGVLYFL 300
           GYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGG KVVVVPHSMGVLYFL
Sbjct: 241 GYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFL 300

Query: 301 YFMKWVEAPPAIGGGGGGQGWCGKHIKAIMNIGSTFLGTPKAVSKIISGEDRSIASLRAM 360
           +FMKWVEAPP + GGGGG GWC K+IKAIMNIGSTFLGTPKAVSKI+S EDR IA LRAM
Sbjct: 301 HFMKWVEAPPPM-GGGGGLGWCAKYIKAIMNIGSTFLGTPKAVSKILSAEDRHIALLRAM 360

Query: 361 TPGFLKSEILGFQTLEHMLRASRTWDSTASLLPKGGETFWGDLDWSPEDVQNCDLEKVDT 420
           TPGFLKSEILGFQTLEHMLR SRTWDSTASLLPKGGET WGDLDWSPEDVQNCDLEK DT
Sbjct: 361 TPGFLKSEILGFQTLEHMLRVSRTWDSTASLLPKGGETIWGDLDWSPEDVQNCDLEKADT 420

Query: 421 HSFRNETDSNDSERQKREGFQQKEAVHYGRIISFSREAATRPSSELSTLTSQEILDSGSS 480
           HSFRNE DSNDSE +K  GFQ+KEAVHYGR+ISF +EAATRPSSELSTL S+E++DSGSS
Sbjct: 421 HSFRNENDSNDSEIKK--GFQEKEAVHYGRMISFGKEAATRPSSELSTLYSEELMDSGSS 480

Query: 481 NDSSFSCSDVWTEYDEMSRESIQKVSENKAYTAKTVFDLLRVVAPRMMQRVESHFSHGIA 540
           NDSSFSCSD WTEYDEMSRES++KVSENKAYTAKTVFDLLRVVAP+MMQR++SHFSHGIA
Sbjct: 481 NDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSHFSHGIA 540

Query: 541 EDLDDPKYAHDHKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSSDKCRRLPFR 600
           EDLDDPKYAH +KYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSS DKC+ +PFR
Sbjct: 541 EDLDDPKYAH-YKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFR 600

Query: 601 IDLSVDGKENGCLRSGMYFVDGDDCVPVLSSGFMCAKGWRNTTRFNPSGSPTYVREYMHK 660
           IDLS+DG+ +GCLRSGMYFVDGDD VPV+SSGFMCAKGWRN TRFNPSGSPTYVREY HK
Sbjct: 601 IDLSIDGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHK 660

Query: 661 APASLLEGRGVESSAHVDIMGNVKLIEDILRVAAGASGTEIGGDRIYSDILTLSERINIK 716
           APASLLEGRG+ESSAHVDIMGN KLIEDILRVAAGA+G EIGGDRIYSDILTLSERIN+K
Sbjct: 661 APASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSERINLK 694

BLAST of MS005133 vs. NCBI nr
Match: XP_023512597.1 (putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1224.2 bits (3166), Expect = 0.0e+00
Identity = 600/721 (83.22%), Postives = 647/721 (89.74%), Query Frame = 0

Query: 1   MASVLRLRKLYYVDPVKSSSMG---INPNEKSEDKRNVIVAYVKKLLEKKQEKDKRKKKK 60
           MASVLRLRKLYYV+PVK+SSMG   I+   KSEDK NV+VAYVKK+L+KKQ+ D++KK+ 
Sbjct: 1   MASVLRLRKLYYVNPVKNSSMGIQSIDDAHKSEDKPNVVVAYVKKMLQKKQDNDRKKKRN 60

Query: 61  NIKNKGGGKEWKCLDNCCWILGYMCTIWWVLFFLYHYLP---PGFPVIDSPGSVLKREGV 120
              NKG  +EWKCLDNCCWI+GYMCT WW++FF+YHYLP   PGFP IDSPG+VLKR+GV
Sbjct: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGTVLKRDGV 120

Query: 121 SALHPVVLVPGLVTGGLELWEGKPCAKGLFRKRLWGGSFSETLKRLENFKANLHSKVHIL 180
           S LHPVVLVPG+VTGGLELW+GKPCAKGLFRKRLWGGSF+ETLK                
Sbjct: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLK---------------- 180

Query: 181 RTELKICRPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAGDYFAQGYFVWAVLIENLAKI 240
                  RPLCWLEHLSLDNETGLDPPGIR+RPVEGLVA DYF  GYFVWAVLIENLAKI
Sbjct: 181 -------RPLCWLEHLSLDNETGLDPPGIRIRPVEGLVAADYFTHGYFVWAVLIENLAKI 240

Query: 241 GYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGEKVVVVPHSMGVLYFL 300
           GYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGG KVVVVPHSMGVLYFL
Sbjct: 241 GYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFL 300

Query: 301 YFMKWVEAPPAIGGGGGGQGWCGKHIKAIMNIGSTFLGTPKAVSKIISGEDRSIASLRAM 360
           +FMKWVEAPP + GGGGGQGWC K+IKAIMNIGSTFLGTPKAVSKI+S EDR IA LRAM
Sbjct: 301 HFMKWVEAPPPM-GGGGGQGWCAKYIKAIMNIGSTFLGTPKAVSKILSAEDRHIALLRAM 360

Query: 361 TPGFLKSEILGFQTLEHMLRASRTWDSTASLLPKGGETFWGDLDWSPEDVQNCDLEKVDT 420
           TPGFLKSEILGFQTLEHMLR SRTWDSTASLLPKGGET WGDLDWSPEDVQNCDLE VDT
Sbjct: 361 TPGFLKSEILGFQTLEHMLRVSRTWDSTASLLPKGGETIWGDLDWSPEDVQNCDLETVDT 420

Query: 421 HSFRNETDSNDSERQKREGFQQKEAVHYGRIISFSREAATRPSSELSTLTSQEILDSGSS 480
           HSFRNE DSNDSE  K  G Q+KEAVHYGR+ISF +EAATRPSSELSTL S+E++DSGSS
Sbjct: 421 HSFRNENDSNDSEINK--GLQEKEAVHYGRMISFGKEAATRPSSELSTLYSEELMDSGSS 480

Query: 481 NDSSFSCSDVWTEYDEMSRESIQKVSENKAYTAKTVFDLLRVVAPRMMQRVESHFSHGIA 540
           NDSSFSCSD WTEYDEMSRES++KVSENKAYTAKTVFDLLRVVAP+MMQR++SHFSHGIA
Sbjct: 481 NDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSHFSHGIA 540

Query: 541 EDLDDPKYAHDHKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSSDKCRRLPFR 600
           EDLDDPKYAH +KYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSS DKC+ +PFR
Sbjct: 541 EDLDDPKYAH-YKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFR 600

Query: 601 IDLSVDGKENGCLRSGMYFVDGDDCVPVLSSGFMCAKGWRNTTRFNPSGSPTYVREYMHK 660
           IDLS+DG+ +GCLRSGMYFVDGDD VPV+SSGFMCAKGWRN TRFNPSGSPTYVREY HK
Sbjct: 601 IDLSIDGEGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHK 660

Query: 661 APASLLEGRGVESSAHVDIMGNVKLIEDILRVAAGASGTEIGGDRIYSDILTLSERINIK 716
           APASLLEGRG+ESSAHVDIMGN KLIEDILRVAAGA+G EIGG+RIYSDILTLSERIN+K
Sbjct: 661 APASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGERIYSDILTLSERINLK 694

BLAST of MS005133 vs. ExPASy Swiss-Prot
Match: Q9FYC7 (Putative phospholipid:diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=PDAT2 PE=3 SV=1)

HSP 1 Score: 839.3 bits (2167), Expect = 3.2e-242
Identity = 413/652 (63.34%), Postives = 499/652 (76.53%), Query Frame = 0

Query: 70  CLDNCCWILGYMCTIWWVLFFLYHY--LPPGFPVIDSPGSVLKREGVSALHPVVLVPGLV 129
           C+D+CCW++GY+CT WW+L FLYH   +P      +SPG+ L R+GV A HPV+LVPG+V
Sbjct: 41  CVDSCCWLIGYLCTAWWLLLFLYHSVPVPAMLQAPESPGTRLSRDGVKAFHPVILVPGIV 100

Query: 130 TGGLELWEGKPCAKGLFRKRLWGGSFSETLKRLENFKANLHSKVHILRTELKICRPLCWL 189
           TGGLELWEG+PCA+GLFRKRLWG SFSE L+                       RPLCWL
Sbjct: 101 TGGLELWEGRPCAEGLFRKRLWGASFSEILR-----------------------RPLCWL 160

Query: 190 EHLSLDNETGLDPPGIRVRPVEGLVAGDYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYD 249
           EHLSLD+ETGLDP GIRVR V GLVA DYFA  YF WAVLIENLAKIGY+GKNLHMA+YD
Sbjct: 161 EHLSLDSETGLDPSGIRVRAVPGLVAADYFAPCYFAWAVLIENLAKIGYEGKNLHMASYD 220

Query: 250 WRIAFQNTEVRDRALSRLKSKIEVMYETNGGEKVVVVPHSMGVLYFLYFMKWVEAPPAIG 309
           WR++F NTEVRD++LSRLKSKIE+MY TNG +KVVVVPHSMG +YFL+F+KWVE P   G
Sbjct: 221 WRLSFHNTEVRDQSLSRLKSKIELMYATNGFKKVVVVPHSMGAIYFLHFLKWVETPLPDG 280

Query: 310 GGGGGQGWCGKHIKAIMNIGSTFLGTPKAVSKIISGEDRSIASLRAMTPGFLKSEILGFQ 369
           GGGGG GWC KHIK+++NIG  FLG PKAVS ++S E + IA  R++ PG L SE+L  Q
Sbjct: 281 GGGGGPGWCAKHIKSVVNIGPAFLGVPKAVSNLLSAEGKDIAYARSLAPGLLDSELLKLQ 340

Query: 370 TLEHMLRASRTWDSTASLLPKGGETFWGDLDWSPEDVQNCDLEKVDTHSFRNETDSNDSE 429
           TLEH++R S +WDS  SLLPKGGE  WGDLD   E+  NC   K  + S  + ++ +   
Sbjct: 341 TLEHLMRMSHSWDSIVSLLPKGGEAIWGDLDSHAEEGLNCIYSKRKS-SQLSLSNLHKQN 400

Query: 430 RQKREGFQQKEAVHYGRIISFSREAATRPSSELSTLTSQEILD-SGSSNDSSFSCSDVWT 489
              +   + KE   YGRI+SF + A+  PSS+LSTL  +E+    G+SNDS+ SC + W+
Sbjct: 401 YSLKPVSRVKEPAKYGRIVSFGKRASELPSSQLSTLNVKELSRVDGNSNDST-SCGEFWS 460

Query: 490 EYDEMSRESIQKVSENKAYTAKTVFDLLRVVAPRMMQRVESHFSHGIAEDLDDPKYAHDH 549
           EY+EMSRESI KV+EN AYTA TV DLLR +AP+MM+R E+HFSHGIA+DLDDPKY H +
Sbjct: 461 EYNEMSRESIVKVAENTAYTATTVLDLLRFIAPKMMRRAEAHFSHGIADDLDDPKYGH-Y 520

Query: 550 KYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYD--SSSDKCR-RLPFRIDLSVDGKE 609
           KYWSNPLETKLP+AP+ME+YCLYGVGIPTERSYIY   +SS KC+  +PFRID S+DG +
Sbjct: 521 KYWSNPLETKLPEAPEMEMYCLYGVGIPTERSYIYKLATSSGKCKSSIPFRIDGSLDG-D 580

Query: 610 NGCLRSGMYFVDGDDCVPVLSSGFMCAKGWRNTTRFNPSGSPTYVREYMHKAPASLLEGR 669
           + CL+ G  F DGD+ VPV+S+GFMCAKGWR  TRFNPSG  T++REY HK P SLLE R
Sbjct: 581 DVCLKGGTRFADGDESVPVISAGFMCAKGWRGKTRFNPSGMDTFLREYKHKPPGSLLESR 640

Query: 670 GVESSAHVDIMGNVKLIEDILRVAAGASGTEIGGDRIYSDILTLSERINIKL 716
           G ES AHVDIMGNV LIED+LR+AAGASG EIGGDRIYSD++ +SERI+IKL
Sbjct: 641 GTESGAHVDIMGNVGLIEDVLRIAAGASGQEIGGDRIYSDVMRMSERISIKL 665

BLAST of MS005133 vs. ExPASy Swiss-Prot
Match: Q9FNA9 (Phospholipid:diacylglycerol acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=PDAT1 PE=2 SV=1)

HSP 1 Score: 767.3 bits (1980), Expect = 1.5e-220
Identity = 382/684 (55.85%), Postives = 474/684 (69.30%), Query Frame = 0

Query: 45  EKKQEKDKRKKKKNIKNKGGGKEWKCLDNCCWILGYMCTIWWVLFFLYHYLPPGF----- 104
           E  Q+K     K + K   GG +W C+D+CCW +G +C  WW L FLY+ +P  F     
Sbjct: 25  EDSQKKPHESSKSHHKKSNGGGKWSCIDSCCWFIGCVCVTWWFLLFLYNAMPASFPQYVT 84

Query: 105 -----PVIDSPGSVLKREGVSALHPVVLVPGLVTGGLELWEGKPCAKGLFRKRLWGGSFS 164
                P+ D PG  LK+EG+ A HPVV +PG+VTGGLELWEGK CA GLFRKRLWGG+F 
Sbjct: 85  ERITGPLPDPPGVKLKKEGLKAKHPVVFIPGIVTGGLELWEGKQCADGLFRKRLWGGTFG 144

Query: 165 ETLKRLENFKANLHSKVHILRTELKICRPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAG 224
           E  K                       RPLCW+EH+SLDNETGLDP GIRVR V GLVA 
Sbjct: 145 EVYK-----------------------RPLCWVEHMSLDNETGLDPAGIRVRAVSGLVAA 204

Query: 225 DYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYE 284
           DYFA GYFVWAVLI NLA IGY+ KN++MAAYDWR++FQNTEVRD+ LSR+KS IE+M  
Sbjct: 205 DYFAPGYFVWAVLIANLAHIGYEEKNMYMAAYDWRLSFQNTEVRDQTLSRMKSNIELMVS 264

Query: 285 TNGGEKVVVVPHSMGVLYFLYFMKWVEAPPAIGGGGGGQGWCGKHIKAIMNIGSTFLGTP 344
           TNGG+K V+VPHSMGVLYFL+FMKWVEAP  + GGGGG  WC K+IKA+MNIG  FLG P
Sbjct: 265 TNGGKKAVIVPHSMGVLYFLHFMKWVEAPAPL-GGGGGPDWCAKYIKAVMNIGGPFLGVP 324

Query: 345 KAVSKIISGEDRSIASLRAMTPGFLKSEILGFQTLEHMLRASRTWDSTASLLPKGGETFW 404
           KAV+ + S E + +A  RA+ PGFL ++I   QTL+H++R +RTWDST S+LPKGG+T W
Sbjct: 325 KAVAGLFSAEAKDVAVARAIAPGFLDTDIFRLQTLQHVMRMTRTWDSTMSMLPKGGDTIW 384

Query: 405 GDLDWSPEDVQNCDLEKVDTHSFRNETDSNDSERQKREGFQQKEAVHYGRIISFSREAAT 464
           G LDWSPE    C  +K   +    E   N        G  +K  V+YGR+ISF +E A 
Sbjct: 385 GGLDWSPEKGHTCCGKKQKNNETCGEAGEN--------GVSKKSPVNYGRMISFGKEVAE 444

Query: 465 RPSSELSTLTSQEILDSGSSNDSSFSCSDVWTEYDEMSRESIQKVSENKAYTAKTVFDLL 524
              SE++ +  +  +   S    + +C DVWTEY +M    I+ ++E K YTA    DLL
Sbjct: 445 AAPSEINNIDFRGAVKGQSI--PNHTCRDVWTEYHDMGIAGIKAIAEYKVYTAGEAIDLL 504

Query: 525 RVVAPRMMQRVESHFSHGIAEDLDDPKYAHDHKYWSNPLETKLPDAPDMEIYCLYGVGIP 584
             VAP+MM R  +HFS+GIA+DLDD KY  D KYWSNPLETKLP+AP+MEIY LYGVGIP
Sbjct: 505 HYVAPKMMARGAAHFSYGIADDLDDTKY-QDPKYWSNPLETKLPNAPEMEIYSLYGVGIP 564

Query: 585 TERSYIY--DSSSDKCRRLPFRIDLSV-DGKENGCLRSGMYFVDGDDCVPVLSSGFMCAK 644
           TER+Y+Y  + S D C  +PF+I  S  +  E+ CL++G+Y VDGD+ VPVLS+G+MCAK
Sbjct: 565 TERAYVYKLNQSPDSC--IPFQIFTSAHEEDEDSCLKAGVYNVDGDETVPVLSAGYMCAK 624

Query: 645 GWRNTTRFNPSGSPTYVREYMHKAPASLLEGRGVESSAHVDIMGNVKLIEDILRVAAGAS 704
            WR  TRFNPSG  TY+REY H  PA+LLEGRG +S AHVDIMGN  LIEDI+RVAAG +
Sbjct: 625 AWRGKTRFNPSGIKTYIREYNHSPPANLLEGRGTQSGAHVDIMGNFALIEDIMRVAAGGN 671

Query: 705 GTEIGGDRIYSDILTLSERINIKL 716
           G++IG D+++S I   SERI++KL
Sbjct: 685 GSDIGHDQVHSGIFEWSERIDLKL 671

BLAST of MS005133 vs. ExPASy Swiss-Prot
Match: P40345 (Phospholipid:diacylglycerol acyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=LRO1 PE=1 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 9.6e-82
Identity = 189/588 (32.14%), Postives = 291/588 (49.49%), Query Frame = 0

Query: 108 VLKREGVSALHPVVLVPGLVTGGLELW----EGKPCAKGLFRKRLWGGSFSETLKRLENF 167
           +L+   + A HPVV+VPG+++ G+E W    + +  +   FRKRLWG             
Sbjct: 163 LLRDYNIEAKHPVVMVPGVISTGIESWGVIGDDECDSSAHFRKRLWG------------- 222

Query: 168 KANLHSKVHILRTELKICRPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAGDYFAQGYFV 227
                   ++LRT   +   +CWL+H+ LD ETGLDPP   +R  +G  + DYF  GY++
Sbjct: 223 ------SFYMLRT--MVMDKVCWLKHVMLDPETGLDPPNFTLRAAQGFESTDYFIAGYWI 282

Query: 228 WAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGEKVVV 287
           W  + +NL  IGY+   +  AAYDWR+A+ + E RDR  ++LK +IE+ ++ + GEKV +
Sbjct: 283 WNKVFQNLGVIGYEPNKMTSAAYDWRLAYLDLERRDRYFTKLKEQIELFHQLS-GEKVCL 342

Query: 288 VPHSMGVLYFLYFMKWVEAPPAIGGGGGGQGWCGKHIKAIMNIGSTFLGTPKAVSKIISG 347
           + HSMG     YFMKWVEA   +  G GG+GW  +HI + +N   T LG PKAV  +ISG
Sbjct: 343 IGHSMGSQIIFYFMKWVEAEGPL-YGNGGRGWVNEHIDSFINAAGTLLGAPKAVPALISG 402

Query: 348 EDRSIASLRAMTPGFLKSEILGFQTLEHMLRASRTWDSTASLLPKGGETFWGDLDWSPED 407
           E +    L  +    L+     F +    ++  +TW    S+LPKG E  WGD+  S ED
Sbjct: 403 EMKDTIQLNTLAMYGLEK----FFSRIERVKMLQTWGGIPSMLPKGEEVIWGDMKSSSED 462

Query: 408 VQNCDLEKVDTHSFRNETDSNDSERQKREGFQQKEAVHYGRIISFSREAATRPSSELSTL 467
             N            N TD+                  YG  I F R             
Sbjct: 463 ALN------------NNTDT------------------YGNFIRFER------------- 522

Query: 468 TSQEILDSGSSNDSSFSCSDVWTEYDEMSRESIQKVSENKAYTAKTVFDLLRVVAPRMMQ 527
                           + SD +                NK  T K   ++   ++P  +Q
Sbjct: 523 ----------------NTSDAF----------------NKNLTMKDAINMTLSISPEWLQ 582

Query: 528 -RVESHFSHGIAEDLDD-PKYAHDHKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIY 587
            RV   +S G +++ ++  K    HK+WSNP+E  LP+AP M+IYC+YGV  PTER+Y+Y
Sbjct: 583 RRVHEQYSFGYSKNEEELRKNELHHKHWSNPMEVPLPEAPHMKIYCIYGVNNPTERAYVY 637

Query: 588 DSSSDKCRRLPFRIDLSVDGKENGCLRSGMYFVDGDDCVPVLSSGFMCAKGWRNTTRFNP 647
               D        ++L++D +     +  ++  +GD  VP+++   MC K  +  + +NP
Sbjct: 643 KEEDDSS-----ALNLTIDYES----KQPVFLTEGDGTVPLVAHS-MCHKWAQGASPYNP 637

Query: 648 SGSPTYVREYMHKAPASLLEGRGVESSAHVDIMGNVKLIEDILRVAAG 690
           +G    + E  H+     + G G +S+ HVDI+G+ +L + IL++A+G
Sbjct: 703 AGINVTIVEMKHQPDRFDIRG-GAKSAEHVDILGSAELNDYILKIASG 637

BLAST of MS005133 vs. ExPASy Swiss-Prot
Match: O94680 (Phospholipid:diacylglycerol acyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=plh1 PE=1 SV=2)

HSP 1 Score: 302.4 bits (773), Expect = 1.4e-80
Identity = 204/609 (33.50%), Postives = 295/609 (48.44%), Query Frame = 0

Query: 106 GSVLKREGVSALHPVVLVPGLVTGGLELWEGKPCAKGLFRKRLWGGSFSETLKRLENFKA 165
           G  +  EG  + HPV++VPG+++ GLE W    C+   FRKRLWG             KA
Sbjct: 134 GLDMYNEGYRSDHPVIMVPGVISSGLESWSFNNCSIPYFRKRLWGS--------WSMLKA 193

Query: 166 NLHSKVHILRTELKICRPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAGDYFAQGYFVWA 225
               K              CWLEHL LD +TGLDP GI++R  +G  A D+F  GY++W+
Sbjct: 194 MFLDK-------------QCWLEHLMLDKKTGLDPKGIKLRAAQGFEAADFFITGYWIWS 253

Query: 226 VLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGEKVVVVP 285
            +IENLA IGY+  N+  A+YDWR+++ N E RD+  S+LK  IE     +  +KVV++ 
Sbjct: 254 KVIENLAAIGYEPNNMLSASYDWRLSYANLEERDKYFSKLKMFIEYSNIVH-KKKVVLIS 313

Query: 286 HSMGVLYFLYFMKWVEAPPAIGGGGGGQGWCGKHIKAIMNIGSTFLGTPKAVSKIISGED 345
           HSMG     YF KWVEA    G G GG  W   HI+A +NI  + +G PK V+ ++SGE 
Sbjct: 314 HSMGSQVTYYFFKWVEAE---GYGNGGPTWVNDHIEAFINISGSLIGAPKTVAALLSGEM 373

Query: 346 RSIASLRAMTPGFLKSEILGFQTLEHMLRASRTWDSTASLLPKGGETFWGDLDWSPEDVQ 405
           +  A L   +   L+     F +        RT    +S+LPKGG+  WG+  W+P+D+ 
Sbjct: 374 KDTAQLNQFSVYGLEK----FFSRSERAMMVRTMGGVSSMLPKGGDVVWGNASWAPDDLN 433

Query: 406 NCDLEKVDTHSFRNETDSNDSERQKREGFQQKEAVHYGRIISFSREAATRPSSELSTLTS 465
             +        +R + D +  E      F   +A+ +                 L  +T 
Sbjct: 434 QTNFSNGAIIRYREDIDKDHDE------FDIDDALQF-----------------LKNVTD 493

Query: 466 QEILDSGSSNDSSFSCSDVWTEYDEMSRESIQKVSENKAYTAKTVFDLLRVVAPRMMQRV 525
            +     + N   +S    WTE         ++V +N    +K                 
Sbjct: 494 DDFKVMLAKN---YSHGLAWTE---------KEVLKNNEMPSK----------------- 553

Query: 526 ESHFSHGIAEDLDDPKYAHDHKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSS 585
                                  W NPLET LP APDM+IYC++GVG PTER Y Y ++ 
Sbjct: 554 -----------------------WINPLETSLPYAPDMKIYCVHGVGKPTERGYYYTNNP 613

Query: 586 DKCRRLPFRIDLSV-DGKENGCLRSGMYFVDGDDCVPVLSSGFMCAKGWRNTTRFNPSGS 645
           +     P  ID SV DG +   + +G+   DGD  +P+L+ G +C K W+ T RFNP+ +
Sbjct: 614 E---GQPV-IDSSVNDGTK---VENGIVMDDGDGTLPILALGLVCNKVWQ-TKRFNPANT 627

Query: 646 PTYVREYMHKAPASLLEGRGVESSAHVDIMGNVKLIEDILRVAAGASGTEIGGDRIYSDI 705
                E  H+  A  L G G  S+ HVDI+G+ +L E IL+V++G  G  +  +R  SDI
Sbjct: 674 SITNYEIKHEPAAFDLRG-GPRSAEHVDILGHSELNEIILKVSSG-HGDSV-PNRYISDI 627

Query: 706 LTLSERINI 714
             +   IN+
Sbjct: 734 QEIINEINL 627

BLAST of MS005133 vs. ExPASy Swiss-Prot
Match: Q8WMP9 (Phospholipase A2 group XV OS=Bos taurus OX=9913 GN=PLA2G15 PE=1 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 4.3e-13
Identity = 81/284 (28.52%), Postives = 121/284 (42.61%), Query Frame = 0

Query: 88  LFFLYHYLPPGFPVIDSPGSVLKREGVSALHPVVLVPGLVTGGLELWEGKPCAKGLFRKR 147
           L FL     P FP    P             PVVLVPG +   LE    KP         
Sbjct: 16  LLFLLMLADPAFPAGSRP-------------PVVLVPGDMGNQLEAKLDKPSVVHYV--- 75

Query: 148 LWGGSFSETLKRLENFKANLHSKVHILRTELKICRPL---CWLEHLSL----DNETGLDP 207
                     KR +++          L   L++  P+   CW++++ L     + T   P
Sbjct: 76  --------CSKRTDHY--------FTLWLNLELLLPVIIDCWIDNVRLIYNQTSHTTQFP 135

Query: 208 PGIRVRPVEGLVAGDYFAQGYF---------VWAVLIENLAKIGYD-GKNLHMAAYDWRI 267
            G+ VR V G   GD F+  +              ++E+L   GY+ GK++  A YDWR 
Sbjct: 136 EGVDVR-VPGF--GDTFSMEFLDPSKSSVGSYLHTMVESLVSWGYERGKDVRGAPYDWRR 195

Query: 268 AFQNTEVRDRALSRLKSKIEVMYETNGGEKVVVVPHSMGVLYFLYFMKWVEAPPAIGGGG 327
           A         AL ++   IE MY+  GG  VV+V HSMG +Y LYF++    P       
Sbjct: 196 APNENGPYFLALRKM---IEEMYQLYGG-PVVLVAHSMGNMYMLYFLQ--HQP------- 249

Query: 328 GGQGWCGKHIKAIMNIGSTFLGTPKAVSKIISGEDRSIASLRAM 355
             Q W  K+I+A + +G  + G PK +  + SG++  I  +R++
Sbjct: 256 --QDWKDKYIRAFVALGPPWGGVPKTLRVLASGDNNRIPVIRSL 249

BLAST of MS005133 vs. ExPASy TrEMBL
Match: A0A6J1CI27 (putative phospholipid:diacylglycerol acyltransferase 2 OS=Momordica charantia OX=3673 GN=LOC111011365 PE=4 SV=1)

HSP 1 Score: 1402.9 bits (3630), Expect = 0.0e+00
Identity = 691/715 (96.64%), Postives = 692/715 (96.78%), Query Frame = 0

Query: 1   MASVLRLRKLYYVDPVKSSSMGINPNEKSEDKRNVIVAYVKKLLEKKQEKDKRKKKKNIK 60
           MASVLRLRKLYYVDPVKSSSMGINPNEKSEDKRNVIVAYVKKLLEKKQEKDKRKKKKNIK
Sbjct: 1   MASVLRLRKLYYVDPVKSSSMGINPNEKSEDKRNVIVAYVKKLLEKKQEKDKRKKKKNIK 60

Query: 61  NKGGGKEWKCLDNCCWILGYMCTIWWVLFFLYHYLPPGFPVIDSPGSVLKREGVSALHPV 120
           NKGGGKEWKCLDNCCWILGYMCTIWWVLFFLYHYLPPGFPVIDSPGSVLKREGVSALHPV
Sbjct: 61  NKGGGKEWKCLDNCCWILGYMCTIWWVLFFLYHYLPPGFPVIDSPGSVLKREGVSALHPV 120

Query: 121 VLVPGLVTGGLELWEGKPCAKGLFRKRLWGGSFSETLKRLENFKANLHSKVHILRTELKI 180
           VLVPGLVTGGLELWEGKPCAKGLFRKRLWGGSFSETLK                      
Sbjct: 121 VLVPGLVTGGLELWEGKPCAKGLFRKRLWGGSFSETLK---------------------- 180

Query: 181 CRPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAGDYFAQGYFVWAVLIENLAKIGYDGKN 240
            RPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAGDYFAQGYFVWAVLIENLAKIGYDGKN
Sbjct: 181 -RPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAGDYFAQGYFVWAVLIENLAKIGYDGKN 240

Query: 241 LHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGEKVVVVPHSMGVLYFLYFMKWV 300
           LHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGEKVVVVPHSMGVLYFLYFMKWV
Sbjct: 241 LHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGEKVVVVPHSMGVLYFLYFMKWV 300

Query: 301 EAPPAIGGGGGGQGWCGKHIKAIMNIGSTFLGTPKAVSKIISGEDRSIASLRAMTPGFLK 360
           EAPPAIGGGGGGQGWCGKHIKAIMNIGSTFLGTPKAVSKIISGEDRSIASLRAMTPGFLK
Sbjct: 301 EAPPAIGGGGGGQGWCGKHIKAIMNIGSTFLGTPKAVSKIISGEDRSIASLRAMTPGFLK 360

Query: 361 SEILGFQTLEHMLRASRTWDSTASLLPKGGETFWGDLDWSPEDVQNCDLEKVDTHSFRNE 420
           SEILGFQTLEHMLRASRTWDSTASLLPKGGETFWGDLDWSPEDVQNCDLEKVDTHSFRNE
Sbjct: 361 SEILGFQTLEHMLRASRTWDSTASLLPKGGETFWGDLDWSPEDVQNCDLEKVDTHSFRNE 420

Query: 421 TDSNDSERQKREGFQQKEAVHYGRIISFSREAATRPSSELSTLTSQEILDSGSSNDSSFS 480
           TDSNDSERQKREGFQQKEAVHYGRIISFSREAATRPSSELSTLTSQEILDSGSSNDSSFS
Sbjct: 421 TDSNDSERQKREGFQQKEAVHYGRIISFSREAATRPSSELSTLTSQEILDSGSSNDSSFS 480

Query: 481 CSDVWTEYDEMSRESIQKVSENKAYTAKTVFDLLRVVAPRMMQRVESHFSHGIAEDLDDP 540
           CSDVWTEYDEMSRESIQKVSENKAYTAKTVFDLLRVVAPRMMQRVESHFSHGIAEDLDDP
Sbjct: 481 CSDVWTEYDEMSRESIQKVSENKAYTAKTVFDLLRVVAPRMMQRVESHFSHGIAEDLDDP 540

Query: 541 KYAHDHKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSSDKCRRLPFRIDLSVD 600
           KYAHDHKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSSDKCRRLPFRIDLSVD
Sbjct: 541 KYAHDHKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSSDKCRRLPFRIDLSVD 600

Query: 601 GKENGCLRSGMYFVDGDDCVPVLSSGFMCAKGWRNTTRFNPSGSPTYVREYMHKAPASLL 660
           GKENGCLRSGMYFVDGDDCVPVLSSGFMCAKGWRNTTRFNPSGSPTYVREYMHKAPASLL
Sbjct: 601 GKENGCLRSGMYFVDGDDCVPVLSSGFMCAKGWRNTTRFNPSGSPTYVREYMHKAPASLL 660

Query: 661 EGRGVESSAHVDIMGNVKLIEDILRVAAGASGTEIGGDRIYSDILTLSERINIKL 716
           EGRGVESSAHVDIMGNVKLIEDILRVAAGASGTE+GGDRIYSDILTLSERINIKL
Sbjct: 661 EGRGVESSAHVDIMGNVKLIEDILRVAAGASGTEVGGDRIYSDILTLSERINIKL 692

BLAST of MS005133 vs. ExPASy TrEMBL
Match: A0A6J1JAN3 (putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucurbita maxima OX=3661 GN=LOC111484072 PE=4 SV=1)

HSP 1 Score: 1233.8 bits (3191), Expect = 0.0e+00
Identity = 605/721 (83.91%), Postives = 651/721 (90.29%), Query Frame = 0

Query: 1   MASVLRLRKLYYVDPVKSSSMG---INPNEKSEDKRNVIVAYVKKLLEKKQEKDKRKKKK 60
           MASVLRLRKLYYV+PVK+SSMG   I+   KSEDK NV+VAYVKK+L+KKQ+KD++KK+ 
Sbjct: 1   MASVLRLRKLYYVNPVKNSSMGIQTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRN 60

Query: 61  NIKNKGGGKEWKCLDNCCWILGYMCTIWWVLFFLYHYLP---PGFPVIDSPGSVLKREGV 120
              NKG  +EWKCLDNCCWI+GYMCT WW++FF+YHYLP   PGFP IDSPG+VLKR+GV
Sbjct: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120

Query: 121 SALHPVVLVPGLVTGGLELWEGKPCAKGLFRKRLWGGSFSETLKRLENFKANLHSKVHIL 180
           S LHPVVLVPG+VTGGLELW+GKPCAKGLFRKRLWGGSF+ETLK                
Sbjct: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLK---------------- 180

Query: 181 RTELKICRPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAGDYFAQGYFVWAVLIENLAKI 240
                  RPLCWLEHLSLDNETGLDPPGIR+RPVEGLVA DYF QGYFVWAVLIENLAKI
Sbjct: 181 -------RPLCWLEHLSLDNETGLDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKI 240

Query: 241 GYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGEKVVVVPHSMGVLYFL 300
           GYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGG KVVVVPHSMGVLYFL
Sbjct: 241 GYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFL 300

Query: 301 YFMKWVEAPPAIGGGGGGQGWCGKHIKAIMNIGSTFLGTPKAVSKIISGEDRSIASLRAM 360
           +FMKWVEAPP + GGGGG GWC K+IKAIMNIGSTFLGTPKAVSKI+S EDR IA LRAM
Sbjct: 301 HFMKWVEAPPPM-GGGGGLGWCAKYIKAIMNIGSTFLGTPKAVSKILSAEDRHIALLRAM 360

Query: 361 TPGFLKSEILGFQTLEHMLRASRTWDSTASLLPKGGETFWGDLDWSPEDVQNCDLEKVDT 420
           TPGFLKSEILGFQTLEHMLR SRTWDSTASLLPKGGET WGDLDWSPEDVQNCDLEKVDT
Sbjct: 361 TPGFLKSEILGFQTLEHMLRVSRTWDSTASLLPKGGETIWGDLDWSPEDVQNCDLEKVDT 420

Query: 421 HSFRNETDSNDSERQKREGFQQKEAVHYGRIISFSREAATRPSSELSTLTSQEILDSGSS 480
           HSFRNE DSNDSE +K  G Q+KEAVHYGR+ISF +EAATRPSSELSTL S+E++DSGSS
Sbjct: 421 HSFRNENDSNDSEIKK--GLQEKEAVHYGRMISFGKEAATRPSSELSTLYSEELMDSGSS 480

Query: 481 NDSSFSCSDVWTEYDEMSRESIQKVSENKAYTAKTVFDLLRVVAPRMMQRVESHFSHGIA 540
           NDSSFSCSD WTEYDEMSRES++KVSENKAYTAKTVFDLLRVVAP+MMQR++SHFSHGIA
Sbjct: 481 NDSSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSHFSHGIA 540

Query: 541 EDLDDPKYAHDHKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSSDKCRRLPFR 600
           EDLDDPKYAH +KYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSS DKC+ +PFR
Sbjct: 541 EDLDDPKYAH-YKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFR 600

Query: 601 IDLSVDGKENGCLRSGMYFVDGDDCVPVLSSGFMCAKGWRNTTRFNPSGSPTYVREYMHK 660
           IDLSVDG+ +GCLRSGMYFVDGDDCVPV+SSGFMCAKGWRN TRFNPSGSPTYVREY HK
Sbjct: 601 IDLSVDGEGSGCLRSGMYFVDGDDCVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHK 660

Query: 661 APASLLEGRGVESSAHVDIMGNVKLIEDILRVAAGASGTEIGGDRIYSDILTLSERINIK 716
           APASLLEGRG+ESSAHVDIMGN KLIEDILRVAAGA+G EIGGDRIYSDILTLSERIN+K
Sbjct: 661 APASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSERINLK 694

BLAST of MS005133 vs. ExPASy TrEMBL
Match: A0A6J1FW53 (putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucurbita moschata OX=3662 GN=LOC111448684 PE=4 SV=1)

HSP 1 Score: 1230.3 bits (3182), Expect = 0.0e+00
Identity = 601/721 (83.36%), Postives = 650/721 (90.15%), Query Frame = 0

Query: 1   MASVLRLRKLYYVDPVKSSSMG---INPNEKSEDKRNVIVAYVKKLLEKKQEKDKRKKKK 60
           MASVLRLRKLYYV+PVK+SSMG   I+   KSEDK NV+VAYVKK+L+KKQ+KD++KK+ 
Sbjct: 1   MASVLRLRKLYYVNPVKNSSMGIQTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRN 60

Query: 61  NIKNKGGGKEWKCLDNCCWILGYMCTIWWVLFFLYHYLP---PGFPVIDSPGSVLKREGV 120
              NKG  +EWKCLDNCCWI+GYMCT WW++FF+YHYLP   PGFP IDSPG+VLKR+GV
Sbjct: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120

Query: 121 SALHPVVLVPGLVTGGLELWEGKPCAKGLFRKRLWGGSFSETLKRLENFKANLHSKVHIL 180
           S LHPVVLVPG+VTGGLELW+GKPCAKGLFRKRLWGGSF+ETLK                
Sbjct: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLK---------------- 180

Query: 181 RTELKICRPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAGDYFAQGYFVWAVLIENLAKI 240
                  RPLCWLEHLSLDNETGLDPPGIR+RPVEGLVA DYF QGYFVWAVLIENLAKI
Sbjct: 181 -------RPLCWLEHLSLDNETGLDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKI 240

Query: 241 GYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGEKVVVVPHSMGVLYFL 300
           GYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGG +VVVVPHSMGVLYFL
Sbjct: 241 GYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGNRVVVVPHSMGVLYFL 300

Query: 301 YFMKWVEAPPAIGGGGGGQGWCGKHIKAIMNIGSTFLGTPKAVSKIISGEDRSIASLRAM 360
           +FMKWVEAPP + GGGGG GWC K+IKAIMNIGSTFLGTPKAVSKI+S EDR IA LRAM
Sbjct: 301 HFMKWVEAPPPM-GGGGGLGWCAKYIKAIMNIGSTFLGTPKAVSKILSAEDRHIALLRAM 360

Query: 361 TPGFLKSEILGFQTLEHMLRASRTWDSTASLLPKGGETFWGDLDWSPEDVQNCDLEKVDT 420
           TPGFLKSEILGFQTLEHMLR SRTWDSTASLLPKGGET WGDLDWSPEDVQNCDLEK DT
Sbjct: 361 TPGFLKSEILGFQTLEHMLRVSRTWDSTASLLPKGGETIWGDLDWSPEDVQNCDLEKADT 420

Query: 421 HSFRNETDSNDSERQKREGFQQKEAVHYGRIISFSREAATRPSSELSTLTSQEILDSGSS 480
           HSFRNE DSNDSE +K  GFQ+KEAVHYGR+ISF +EAATRPSSELSTL S+E++DSGSS
Sbjct: 421 HSFRNENDSNDSEIKK--GFQEKEAVHYGRMISFGKEAATRPSSELSTLYSEELMDSGSS 480

Query: 481 NDSSFSCSDVWTEYDEMSRESIQKVSENKAYTAKTVFDLLRVVAPRMMQRVESHFSHGIA 540
           ND+SFSCSD WTEYDEMSRES++KVSENKAYTAKTVFDLLRVVAP+MMQR++SHFSHGIA
Sbjct: 481 NDTSFSCSDGWTEYDEMSRESLRKVSENKAYTAKTVFDLLRVVAPKMMQRMDSHFSHGIA 540

Query: 541 EDLDDPKYAHDHKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSSDKCRRLPFR 600
           EDLDDPKY H +KYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSS DKC+ +PFR
Sbjct: 541 EDLDDPKYGH-YKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFR 600

Query: 601 IDLSVDGKENGCLRSGMYFVDGDDCVPVLSSGFMCAKGWRNTTRFNPSGSPTYVREYMHK 660
           IDLS+DG+ +GCLRSGMYFVDGDDCVPV+SSGFMCAKGWRN TRFNPSGSPTYVREY HK
Sbjct: 601 IDLSIDGEGSGCLRSGMYFVDGDDCVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHK 660

Query: 661 APASLLEGRGVESSAHVDIMGNVKLIEDILRVAAGASGTEIGGDRIYSDILTLSERINIK 716
           APASLLEGRG+ESSAHVDIMGN KLIEDILRVAAGA+G EIGGDRIYSDILTLSERIN+K
Sbjct: 661 APASLLEGRGIESSAHVDIMGNAKLIEDILRVAAGATGAEIGGDRIYSDILTLSERINLK 694

BLAST of MS005133 vs. ExPASy TrEMBL
Match: A0A5A7SR19 (Putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold26G00090 PE=4 SV=1)

HSP 1 Score: 1201.4 bits (3107), Expect = 0.0e+00
Identity = 591/720 (82.08%), Postives = 642/720 (89.17%), Query Frame = 0

Query: 1   MASVLRLRKLYYVDPVKSSSMGINPNE--KSEDKRNVIVAYVKKLLEKKQEKDKRKKKKN 60
           MASVLRLRKLY+V+PVKSSSMG+   E  KSEDKRNVIVAYVKKL++KKQ+KD ++K+  
Sbjct: 1   MASVLRLRKLYFVNPVKSSSMGLQSIEAHKSEDKRNVIVAYVKKLMQKKQDKDDKQKRNK 60

Query: 61  IKNKGGGKEWKCLDNCCWILGYMCTIWWVLFFLYHYLP---PGFPVIDSPGSVLKREGVS 120
             N+G  +EWKCLDNCCWI+GY+CT WW++FFLYHYLP   PGFP IDSPG+VLKREG+S
Sbjct: 61  NTNRGQSREWKCLDNCCWIIGYICTFWWLMFFLYHYLPLSVPGFPAIDSPGAVLKREGIS 120

Query: 121 ALHPVVLVPGLVTGGLELWEGKPCAKGLFRKRLWGGSFSETLKRLENFKANLHSKVHILR 180
             HPVVLVPG+VTGGLELW+GKPCA+GLFRKRLWGGSF+ETLK                 
Sbjct: 121 GRHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLK----------------- 180

Query: 181 TELKICRPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAGDYFAQGYFVWAVLIENLAKIG 240
                 RPLCWLEHLSLDNETGLDPPGIRVRPVEGLVA DYFAQGYFVWAVLIENLAKIG
Sbjct: 181 ------RPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIG 240

Query: 241 YDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGEKVVVVPHSMGVLYFLY 300
           YDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMY TNG  KVVVVPHSMGVLYFL+
Sbjct: 241 YDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYATNGENKVVVVPHSMGVLYFLH 300

Query: 301 FMKWVEAPPAIGGGGGGQGWCGKHIKAIMNIGSTFLGTPKAVSKIISGEDRSIASLRAMT 360
           FMKWVEAPP + GGGGG GWC KHIK+IMNIGSTFLGTPKAVSKI+S EDR +A LRAMT
Sbjct: 301 FMKWVEAPPPM-GGGGGLGWCAKHIKSIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMT 360

Query: 361 PGFLKSEILGFQTLEHMLRASRTWDSTASLLPKGGETFWGDLDWSPEDVQNCDLEKVDTH 420
           PGFLKSEILGFQTLEH+LR SRTWDSTASLLPKGGET WGDLDWSPEDVQNCDL+KVD H
Sbjct: 361 PGFLKSEILGFQTLEHLLRVSRTWDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDAH 420

Query: 421 SFRNETDSNDSERQKREGFQQKEAVHYGRIISFSREAATRPSSELSTLTSQEILDSGSSN 480
           SFRNE DS+DSER+K   FQ++E VHYGRIISFS+EAATRPSSELSTL  +E+ +SG SN
Sbjct: 421 SFRNENDSDDSERKK--CFQEQEPVHYGRIISFSKEAATRPSSELSTLNLEELKNSGGSN 480

Query: 481 DSSFSCSDVWTEYDEMSRESIQKVSENKAYTAKTVFDLLRVVAPRMMQRVESHFSHGIAE 540
           DSSFSCSDVWTEYDEMSR+SI+KVSENKAYT KTVFDLLRVVAP+MMQR++SHFSHGIAE
Sbjct: 481 DSSFSCSDVWTEYDEMSRDSIRKVSENKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAE 540

Query: 541 DLDDPKYAHDHKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSSDKCRRLPFRI 600
           DLDDPKYAH HKYWSNPLETKLPDAP+MEIYCLYGVGIPTERSYIYDSS DKC+ +PFRI
Sbjct: 541 DLDDPKYAH-HKYWSNPLETKLPDAPNMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRI 600

Query: 601 DLSVDGKENGCLRSGMYFVDGDDCVPVLSSGFMCAKGWRNTTRFNPSGSPTYVREYMHKA 660
           DLS++GK +GCLRSGMYFVDGDD VP++SSGFMCAKGWRN TRFNPSGSPTYVREY HKA
Sbjct: 601 DLSIEGKGSGCLRSGMYFVDGDDSVPIVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKA 660

Query: 661 PASLLEGRGVESSAHVDIMGNVKLIEDILRVAAGASGTEIGGDRIYSDILTLSERINIKL 716
           PASLLEGRGVESSAHVDIMGNV LIEDILRVAA   G EIGGD+IYSDILTLSERIN+KL
Sbjct: 661 PASLLEGRGVESSAHVDIMGNVNLIEDILRVAA---GEEIGGDKIYSDILTLSERINLKL 690

BLAST of MS005133 vs. ExPASy TrEMBL
Match: A0A1S3C9K8 (putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucumis melo OX=3656 GN=LOC103497986 PE=4 SV=1)

HSP 1 Score: 1201.4 bits (3107), Expect = 0.0e+00
Identity = 591/720 (82.08%), Postives = 642/720 (89.17%), Query Frame = 0

Query: 1   MASVLRLRKLYYVDPVKSSSMGINPNE--KSEDKRNVIVAYVKKLLEKKQEKDKRKKKKN 60
           MASVLRLRKLY+V+PVKSSSMG+   E  KSEDKRNVIVAYVKKL++KKQ+KD ++K+  
Sbjct: 114 MASVLRLRKLYFVNPVKSSSMGLQSIEAHKSEDKRNVIVAYVKKLMQKKQDKDDKQKRNK 173

Query: 61  IKNKGGGKEWKCLDNCCWILGYMCTIWWVLFFLYHYLP---PGFPVIDSPGSVLKREGVS 120
             N+G  +EWKCLDNCCWI+GY+CT WW++FFLYHYLP   PGFP IDSPG+VLKREG+S
Sbjct: 174 NTNRGQSREWKCLDNCCWIIGYICTFWWLMFFLYHYLPLSVPGFPAIDSPGAVLKREGIS 233

Query: 121 ALHPVVLVPGLVTGGLELWEGKPCAKGLFRKRLWGGSFSETLKRLENFKANLHSKVHILR 180
             HPVVLVPG+VTGGLELW+GKPCA+GLFRKRLWGGSF+ETLK                 
Sbjct: 234 GRHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLK----------------- 293

Query: 181 TELKICRPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAGDYFAQGYFVWAVLIENLAKIG 240
                 RPLCWLEHLSLDNETGLDPPGIRVRPVEGLVA DYFAQGYFVWAVLIENLAKIG
Sbjct: 294 ------RPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIG 353

Query: 241 YDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGEKVVVVPHSMGVLYFLY 300
           YDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMY TNG  KVVVVPHSMGVLYFL+
Sbjct: 354 YDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYATNGENKVVVVPHSMGVLYFLH 413

Query: 301 FMKWVEAPPAIGGGGGGQGWCGKHIKAIMNIGSTFLGTPKAVSKIISGEDRSIASLRAMT 360
           FMKWVEAPP + GGGGG GWC KHIK+IMNIGSTFLGTPKAVSKI+S EDR +A LRAMT
Sbjct: 414 FMKWVEAPPPM-GGGGGLGWCAKHIKSIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMT 473

Query: 361 PGFLKSEILGFQTLEHMLRASRTWDSTASLLPKGGETFWGDLDWSPEDVQNCDLEKVDTH 420
           PGFLKSEILGFQTLEH+LR SRTWDSTASLLPKGGET WGDLDWSPEDVQNCDL+KVD H
Sbjct: 474 PGFLKSEILGFQTLEHLLRVSRTWDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDAH 533

Query: 421 SFRNETDSNDSERQKREGFQQKEAVHYGRIISFSREAATRPSSELSTLTSQEILDSGSSN 480
           SFRNE DS+DSER+K   FQ++E VHYGRIISFS+EAATRPSSELSTL  +E+ +SG SN
Sbjct: 534 SFRNENDSDDSERKK--CFQEQEPVHYGRIISFSKEAATRPSSELSTLNLEELKNSGGSN 593

Query: 481 DSSFSCSDVWTEYDEMSRESIQKVSENKAYTAKTVFDLLRVVAPRMMQRVESHFSHGIAE 540
           DSSFSCSDVWTEYDEMSR+SI+KVSENKAYT KTVFDLLRVVAP+MMQR++SHFSHGIAE
Sbjct: 594 DSSFSCSDVWTEYDEMSRDSIRKVSENKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAE 653

Query: 541 DLDDPKYAHDHKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSSDKCRRLPFRI 600
           DLDDPKYAH HKYWSNPLETKLPDAP+MEIYCLYGVGIPTERSYIYDSS DKC+ +PFRI
Sbjct: 654 DLDDPKYAH-HKYWSNPLETKLPDAPNMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRI 713

Query: 601 DLSVDGKENGCLRSGMYFVDGDDCVPVLSSGFMCAKGWRNTTRFNPSGSPTYVREYMHKA 660
           DLS++GK +GCLRSGMYFVDGDD VP++SSGFMCAKGWRN TRFNPSGSPTYVREY HKA
Sbjct: 714 DLSIEGKGSGCLRSGMYFVDGDDSVPIVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKA 773

Query: 661 PASLLEGRGVESSAHVDIMGNVKLIEDILRVAAGASGTEIGGDRIYSDILTLSERINIKL 716
           PASLLEGRGVESSAHVDIMGNV LIEDILRVAA   G EIGGD+IYSDILTLSERIN+KL
Sbjct: 774 PASLLEGRGVESSAHVDIMGNVNLIEDILRVAA---GEEIGGDKIYSDILTLSERINLKL 803

BLAST of MS005133 vs. TAIR 10
Match: AT3G44830.1 (Lecithin:cholesterol acyltransferase family protein )

HSP 1 Score: 839.3 bits (2167), Expect = 2.2e-243
Identity = 413/652 (63.34%), Postives = 499/652 (76.53%), Query Frame = 0

Query: 70  CLDNCCWILGYMCTIWWVLFFLYHY--LPPGFPVIDSPGSVLKREGVSALHPVVLVPGLV 129
           C+D+CCW++GY+CT WW+L FLYH   +P      +SPG+ L R+GV A HPV+LVPG+V
Sbjct: 41  CVDSCCWLIGYLCTAWWLLLFLYHSVPVPAMLQAPESPGTRLSRDGVKAFHPVILVPGIV 100

Query: 130 TGGLELWEGKPCAKGLFRKRLWGGSFSETLKRLENFKANLHSKVHILRTELKICRPLCWL 189
           TGGLELWEG+PCA+GLFRKRLWG SFSE L+                       RPLCWL
Sbjct: 101 TGGLELWEGRPCAEGLFRKRLWGASFSEILR-----------------------RPLCWL 160

Query: 190 EHLSLDNETGLDPPGIRVRPVEGLVAGDYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYD 249
           EHLSLD+ETGLDP GIRVR V GLVA DYFA  YF WAVLIENLAKIGY+GKNLHMA+YD
Sbjct: 161 EHLSLDSETGLDPSGIRVRAVPGLVAADYFAPCYFAWAVLIENLAKIGYEGKNLHMASYD 220

Query: 250 WRIAFQNTEVRDRALSRLKSKIEVMYETNGGEKVVVVPHSMGVLYFLYFMKWVEAPPAIG 309
           WR++F NTEVRD++LSRLKSKIE+MY TNG +KVVVVPHSMG +YFL+F+KWVE P   G
Sbjct: 221 WRLSFHNTEVRDQSLSRLKSKIELMYATNGFKKVVVVPHSMGAIYFLHFLKWVETPLPDG 280

Query: 310 GGGGGQGWCGKHIKAIMNIGSTFLGTPKAVSKIISGEDRSIASLRAMTPGFLKSEILGFQ 369
           GGGGG GWC KHIK+++NIG  FLG PKAVS ++S E + IA  R++ PG L SE+L  Q
Sbjct: 281 GGGGGPGWCAKHIKSVVNIGPAFLGVPKAVSNLLSAEGKDIAYARSLAPGLLDSELLKLQ 340

Query: 370 TLEHMLRASRTWDSTASLLPKGGETFWGDLDWSPEDVQNCDLEKVDTHSFRNETDSNDSE 429
           TLEH++R S +WDS  SLLPKGGE  WGDLD   E+  NC   K  + S  + ++ +   
Sbjct: 341 TLEHLMRMSHSWDSIVSLLPKGGEAIWGDLDSHAEEGLNCIYSKRKS-SQLSLSNLHKQN 400

Query: 430 RQKREGFQQKEAVHYGRIISFSREAATRPSSELSTLTSQEILD-SGSSNDSSFSCSDVWT 489
              +   + KE   YGRI+SF + A+  PSS+LSTL  +E+    G+SNDS+ SC + W+
Sbjct: 401 YSLKPVSRVKEPAKYGRIVSFGKRASELPSSQLSTLNVKELSRVDGNSNDST-SCGEFWS 460

Query: 490 EYDEMSRESIQKVSENKAYTAKTVFDLLRVVAPRMMQRVESHFSHGIAEDLDDPKYAHDH 549
           EY+EMSRESI KV+EN AYTA TV DLLR +AP+MM+R E+HFSHGIA+DLDDPKY H +
Sbjct: 461 EYNEMSRESIVKVAENTAYTATTVLDLLRFIAPKMMRRAEAHFSHGIADDLDDPKYGH-Y 520

Query: 550 KYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYD--SSSDKCR-RLPFRIDLSVDGKE 609
           KYWSNPLETKLP+AP+ME+YCLYGVGIPTERSYIY   +SS KC+  +PFRID S+DG +
Sbjct: 521 KYWSNPLETKLPEAPEMEMYCLYGVGIPTERSYIYKLATSSGKCKSSIPFRIDGSLDG-D 580

Query: 610 NGCLRSGMYFVDGDDCVPVLSSGFMCAKGWRNTTRFNPSGSPTYVREYMHKAPASLLEGR 669
           + CL+ G  F DGD+ VPV+S+GFMCAKGWR  TRFNPSG  T++REY HK P SLLE R
Sbjct: 581 DVCLKGGTRFADGDESVPVISAGFMCAKGWRGKTRFNPSGMDTFLREYKHKPPGSLLESR 640

Query: 670 GVESSAHVDIMGNVKLIEDILRVAAGASGTEIGGDRIYSDILTLSERINIKL 716
           G ES AHVDIMGNV LIED+LR+AAGASG EIGGDRIYSD++ +SERI+IKL
Sbjct: 641 GTESGAHVDIMGNVGLIEDVLRIAAGASGQEIGGDRIYSDVMRMSERISIKL 665

BLAST of MS005133 vs. TAIR 10
Match: AT5G13640.1 (phospholipid:diacylglycerol acyltransferase )

HSP 1 Score: 767.3 bits (1980), Expect = 1.1e-221
Identity = 382/684 (55.85%), Postives = 474/684 (69.30%), Query Frame = 0

Query: 45  EKKQEKDKRKKKKNIKNKGGGKEWKCLDNCCWILGYMCTIWWVLFFLYHYLPPGF----- 104
           E  Q+K     K + K   GG +W C+D+CCW +G +C  WW L FLY+ +P  F     
Sbjct: 25  EDSQKKPHESSKSHHKKSNGGGKWSCIDSCCWFIGCVCVTWWFLLFLYNAMPASFPQYVT 84

Query: 105 -----PVIDSPGSVLKREGVSALHPVVLVPGLVTGGLELWEGKPCAKGLFRKRLWGGSFS 164
                P+ D PG  LK+EG+ A HPVV +PG+VTGGLELWEGK CA GLFRKRLWGG+F 
Sbjct: 85  ERITGPLPDPPGVKLKKEGLKAKHPVVFIPGIVTGGLELWEGKQCADGLFRKRLWGGTFG 144

Query: 165 ETLKRLENFKANLHSKVHILRTELKICRPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAG 224
           E  K                       RPLCW+EH+SLDNETGLDP GIRVR V GLVA 
Sbjct: 145 EVYK-----------------------RPLCWVEHMSLDNETGLDPAGIRVRAVSGLVAA 204

Query: 225 DYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYE 284
           DYFA GYFVWAVLI NLA IGY+ KN++MAAYDWR++FQNTEVRD+ LSR+KS IE+M  
Sbjct: 205 DYFAPGYFVWAVLIANLAHIGYEEKNMYMAAYDWRLSFQNTEVRDQTLSRMKSNIELMVS 264

Query: 285 TNGGEKVVVVPHSMGVLYFLYFMKWVEAPPAIGGGGGGQGWCGKHIKAIMNIGSTFLGTP 344
           TNGG+K V+VPHSMGVLYFL+FMKWVEAP  + GGGGG  WC K+IKA+MNIG  FLG P
Sbjct: 265 TNGGKKAVIVPHSMGVLYFLHFMKWVEAPAPL-GGGGGPDWCAKYIKAVMNIGGPFLGVP 324

Query: 345 KAVSKIISGEDRSIASLRAMTPGFLKSEILGFQTLEHMLRASRTWDSTASLLPKGGETFW 404
           KAV+ + S E + +A  RA+ PGFL ++I   QTL+H++R +RTWDST S+LPKGG+T W
Sbjct: 325 KAVAGLFSAEAKDVAVARAIAPGFLDTDIFRLQTLQHVMRMTRTWDSTMSMLPKGGDTIW 384

Query: 405 GDLDWSPEDVQNCDLEKVDTHSFRNETDSNDSERQKREGFQQKEAVHYGRIISFSREAAT 464
           G LDWSPE    C  +K   +    E   N        G  +K  V+YGR+ISF +E A 
Sbjct: 385 GGLDWSPEKGHTCCGKKQKNNETCGEAGEN--------GVSKKSPVNYGRMISFGKEVAE 444

Query: 465 RPSSELSTLTSQEILDSGSSNDSSFSCSDVWTEYDEMSRESIQKVSENKAYTAKTVFDLL 524
              SE++ +  +  +   S    + +C DVWTEY +M    I+ ++E K YTA    DLL
Sbjct: 445 AAPSEINNIDFRGAVKGQSI--PNHTCRDVWTEYHDMGIAGIKAIAEYKVYTAGEAIDLL 504

Query: 525 RVVAPRMMQRVESHFSHGIAEDLDDPKYAHDHKYWSNPLETKLPDAPDMEIYCLYGVGIP 584
             VAP+MM R  +HFS+GIA+DLDD KY  D KYWSNPLETKLP+AP+MEIY LYGVGIP
Sbjct: 505 HYVAPKMMARGAAHFSYGIADDLDDTKY-QDPKYWSNPLETKLPNAPEMEIYSLYGVGIP 564

Query: 585 TERSYIY--DSSSDKCRRLPFRIDLSV-DGKENGCLRSGMYFVDGDDCVPVLSSGFMCAK 644
           TER+Y+Y  + S D C  +PF+I  S  +  E+ CL++G+Y VDGD+ VPVLS+G+MCAK
Sbjct: 565 TERAYVYKLNQSPDSC--IPFQIFTSAHEEDEDSCLKAGVYNVDGDETVPVLSAGYMCAK 624

Query: 645 GWRNTTRFNPSGSPTYVREYMHKAPASLLEGRGVESSAHVDIMGNVKLIEDILRVAAGAS 704
            WR  TRFNPSG  TY+REY H  PA+LLEGRG +S AHVDIMGN  LIEDI+RVAAG +
Sbjct: 625 AWRGKTRFNPSGIKTYIREYNHSPPANLLEGRGTQSGAHVDIMGNFALIEDIMRVAAGGN 671

Query: 705 GTEIGGDRIYSDILTLSERINIKL 716
           G++IG D+++S I   SERI++KL
Sbjct: 685 GSDIGHDQVHSGIFEWSERIDLKL 671

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140787.10.0e+0096.64putative phospholipid:diacylglycerol acyltransferase 2 [Momordica charantia][more]
XP_022986276.10.0e+0083.91putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita maxima][more]
XP_022944139.10.0e+0083.36putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita moschata][more]
KAG6570836.10.0e+0083.63putative phospholipid:diacylglycerol acyltransferase 2, partial [Cucurbita argyr... [more]
XP_023512597.10.0e+0083.22putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita pepo subsp. pe... [more]
Match NameE-valueIdentityDescription
Q9FYC73.2e-24263.34Putative phospholipid:diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana O... [more]
Q9FNA91.5e-22055.85Phospholipid:diacylglycerol acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN... [more]
P403459.6e-8232.14Phospholipid:diacylglycerol acyltransferase OS=Saccharomyces cerevisiae (strain ... [more]
O946801.4e-8033.50Phospholipid:diacylglycerol acyltransferase OS=Schizosaccharomyces pombe (strain... [more]
Q8WMP94.3e-1328.52Phospholipase A2 group XV OS=Bos taurus OX=9913 GN=PLA2G15 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CI270.0e+0096.64putative phospholipid:diacylglycerol acyltransferase 2 OS=Momordica charantia OX... [more]
A0A6J1JAN30.0e+0083.91putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucurbita maxima OX=36... [more]
A0A6J1FW530.0e+0083.36putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucurbita moschata OX=... [more]
A0A5A7SR190.0e+0082.08Putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucumis melo var. maku... [more]
A0A1S3C9K80.0e+0082.08putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucumis melo OX=3656 G... [more]
Match NameE-valueIdentityDescription
AT3G44830.12.2e-24363.34Lecithin:cholesterol acyltransferase family protein [more]
AT5G13640.11.1e-22155.85phospholipid:diacylglycerol acyltransferase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 40..60
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 415..436
NoneNo IPR availablePANTHERPTHR11440LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATEDcoord: 45..159
NoneNo IPR availablePANTHERPTHR11440:SF87PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE 2-RELATEDcoord: 45..159
NoneNo IPR availablePANTHERPTHR11440LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATEDcoord: 182..715
NoneNo IPR availablePANTHERPTHR11440:SF87PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE 2-RELATEDcoord: 182..715
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 194..364
e-value: 7.1E-30
score: 106.2
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 115..629
IPR003386Lecithin:cholesterol/phospholipid:diacylglycerol acyltransferasePFAMPF02450LCATcoord: 179..675
e-value: 5.3E-67
score: 226.7

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS005133.1MS005133.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
molecular_function GO:0008374 O-acyltransferase activity