MS005129 (gene) Bitter gourd (TR) v1

Overview
NameMS005129
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionaspartic proteinase-like protein 2
Locationscaffold176: 2898719 .. 2901982 (-)
RNA-Seq ExpressionMS005129
SyntenyMS005129
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGCGATTGTTCGTACCGCAGTGTCGGTGGCCGCCGCGGTGCTGATGCTTCAGGCGGCCACGGTTCTCTGCGGGTTTCCCGCCAAGCTGAGTCTGGAGAGGGCTTTTCCGACGAATCATGGGGTCGAAATGGCTCAACTCCGAGGCCGGGACCGGGCTAGACATGGTAGAATGTTGCAGTCTTCTGGTGGTGTCATTGATTTTCCTGTGGCCGGGACCTATGATCCATTTCTCGTTGGGTAAGGGAAGGGAAGGGAACGAATCTTTTTTAATTTATGCTTTTATCTATCCTTTCGAATTTTTGTTCACCTTTCCTAATTTGAACACATGTCTTGATTTTTGGAACATATTTGGGTTTTTATTCCCGATTGAAACTTCTTTATGAATCGGCTCAAATTGATGCTTTGTTGTTAGTTTAACAAATGTATAACCTCTGGTTTCTTTTTTCCAGAAATACATTATACTTCTGTTATTTTATGCCTTCTCTTTTTCCCAATTATAGTTTTAAAGAACTTGCTAGTTTTACTTTTAAATTTCCCGATATCTTTATGTATCATCAATCATCATGGCCATTGTCCATGAACTGATCTTTCAATACCTTGGGTTCCGGCTCTTTCTTGATACATTAATTACCTTATTCGCTTGCCTAAAATTTTTTGGTTGTTCTACAGGCTTTATTACACTAAAGTGCAGCTAGGTAATCCTCCGAAGGATTTCTTTGTGCAGATCGATACTGGAAGTGATGTTTTGTGGGTTAGCTGCAACTCTTGCAATGGATGCCCAGAAACTAGTGGGCTCCAGGTGATTCTGTGTTACCACGAGTTTTAAGTCCATTTTAAGCTGGTGGAAGTGTCACCAAAGGCTAAGCCCTAACCTTTTGCTGCTACAAATTTTCAATTCAGTTGTTTTCTCCCCTCACAGATTCAGCTCAATTTCTTTGATCCTGCTAGCTCATCAACAGCTTCTCCAGTCTCTTGTTCAGACCAAATTTGCGCCCTTGGAGTTCAGTCCTCTGATTCTGCCTGTTTGGGCCAGAGCAATCAATGTGCTTATGTCTTCCAGTATGGAGATGGGAGTGGAACATCGGGCTTTTACGTTAGGGACATGATGCATCTCGATATAGTAGTTGAGAGTTCTATGACTACAAATTCTTCAGCTTCAGTTGTATTTGGGTGAGCTTATGCTTCATTCGAAAACTAGTCCTACTCATCGTATGTTTTTGTACTTGTATAAACTCCTTTGGTTGGTGAATTATCATTTATTAAACAGGTGTAGTACATCACAGACTGGAGACTTGACTAAGTCGGACAGAGCAGTTGATGGAATCTTCGGGTTCGGGCAACATGACTTGTCTGTAATTTCGCAACTGTCTTCACGAGGAATAGCACCAAAAGTGTTCTCCCACTGCTTGAAAGGAGATGATAGTGGTGGGGGAATATTGGTCCTTGGCGAGATTGTGGAGCCAAATGTTGTTTATACTCCTCTAGTCCCATCACAGTACGTTTTTTCATTTCCCATCAGTAGTTGAACTCCATTTTCATTATTTTGGTCTTGAGGTGTCTCTGAGTAACTTGGTTAACCTACAATCGAAATGTCAGCATTGTAAACTTATCCGAGTATTTGAAGGTTTGGATCAAATAAAGTCCAGAAGCATATGGTTTCTGATTGTTGTTGGCATTAATTTTCAGGCCCCATTATAACTTGAATCTGCAAAGCATCTCTGTTAATGGTCAAATGTTACCCATCAATCCAACTGTCTTTACAACAACCAATGGCCAAGGAACCATAGTTGACTCTGGCACTACTTTGGCATACCTTGCTGAGGAAGCTTTCAACTCTTTTGTTGTTGCTGTGAGTTTTACTTTAAGATGTTTCTCATTGTTCAAATGGTCAAAAGCACCATCATGACGCTTCATGATTCATTCATTATCATTGTTTCACACTGTTATATATATTATTTTCGTTTATAGGAAGGGTGAAGTAGACTCATCCTTGGAAGTTTAATGGATATTGAGTAATTATAACGTTTATTTATCTCTATTTTGTAACTCAGATCACGAATTTAGTTTCACAAACGACACAGCCTGTTCTTCTCAAGGGAAATCAGTGTTATATAACCTCCTCCAGGTTGATTTAATTTACTATCTACATTTCTGTTTGAAGTTAATATATACTGTGAGAATCTGACTCTTCACTTTCGTCTTTGACTATAATAACAGCATCTCTGACACATTTCCTCAAGTAAGCTTAAACTTTGCCGGTGGCGCATCGCTTGTTTTGAGACCTCAGGACTATCTCATACAACAAAGCTCTGCTGTAAGTTTATAGCCATCATTATCTGGACATTGACATTCTCATAGTTGTATAACTGTAATAGGTCATAGTTTTCCTAAAAAACACCAAATTTTCATGCCTTAGAGGTAAGAAGCATTAAATAGAATCAATTTTATTTAATCGTACCTTTATTCCCATAACTTGTATCCTGCTTTGGGAGTGGGTTTGATAAAATTTTCATTGCAGGGTGGAAATACTGTTTGGTGTGTTGGTTTCCAGAAAATCCCAGGTCAAGGGATTACAATTCTAGGAGGTGAGTTCAGGGATTTGATCTTTGAGTTGGATTTTAAAACCATTTACTAACCTAAAATAGTGACATTGCCTCTCTTTTTTCCGGCAGACCTTGTTCTAAAAGACAAAATCTTCATTTATGATTTAGCGAATCAACGAATTGGATGGGCTAACTACGACTGTGAGTTTCGACACCCCTCGTTAAAAGCGTGTTTTGACACATGGATAATGAAATTACATTCGGAACACAAATTTCTTTCCACACTCAAAACATGCACACTAGTTAATTGTACTTTGAATTCTGGAAAGGTTTTTGTGCTGTGAAATATAATTCAACCACACGAATATTATTTCCTATTGCATTAAAAACGAGGTTCACTCATCTTGCTGTGCTACATTAGACCAAAAACCAAAAACTCTTTATTTCTTTCAGATGTCAAATTATATTTTAAGTTGTTTTCAGCTTCAACTTCCAAGCAATAATGAAATCCATTCTGACATGTTTTCTCCAACATATTAGGTTCAATGTCAGTAAATGTTTCCACAGCTTCCAAGACTGGACAGAGTGAGTCTGTCAACGCAGGGCAGCTCAGCGGCAGTGGCTCCGTGGAGAATCAGCCTGACGGACTTATTCTACAGCTAAGCATTCTTGTATTGTTCGTTCAATTATCCATCTTCACCAGCTTCTTCCACTCA

mRNA sequence

ATGGCCGCGATTGTTCGTACCGCAGTGTCGGTGGCCGCCGCGGTGCTGATGCTTCAGGCGGCCACGGTTCTCTGCGGGTTTCCCGCCAAGCTGAGTCTGGAGAGGGCTTTTCCGACGAATCATGGGGTCGAAATGGCTCAACTCCGAGGCCGGGACCGGGCTAGACATGGTAGAATGTTGCAGTCTTCTGGTGGTGTCATTGATTTTCCTGTGGCCGGGACCTATGATCCATTTCTCGTTGGGCTTTATTACACTAAAGTGCAGCTAGGTAATCCTCCGAAGGATTTCTTTGTGCAGATCGATACTGGAAGTGATGTTTTGTGGGTTAGCTGCAACTCTTGCAATGGATGCCCAGAAACTAGTGGGCTCCAGATTCAGCTCAATTTCTTTGATCCTGCTAGCTCATCAACAGCTTCTCCAGTCTCTTGTTCAGACCAAATTTGCGCCCTTGGAGTTCAGTCCTCTGATTCTGCCTGTTTGGGCCAGAGCAATCAATGTGCTTATGTCTTCCAGTATGGAGATGGGAGTGGAACATCGGGCTTTTACGTTAGGGACATGATGCATCTCGATATAGTAGTTGAGAGTTCTATGACTACAAATTCTTCAGCTTCAGTTGTATTTGGGTGTAGTACATCACAGACTGGAGACTTGACTAAGTCGGACAGAGCAGTTGATGGAATCTTCGGGTTCGGGCAACATGACTTGTCTGTAATTTCGCAACTGTCTTCACGAGGAATAGCACCAAAAGTGTTCTCCCACTGCTTGAAAGGAGATGATAGTGGTGGGGGAATATTGGTCCTTGGCGAGATTGTGGAGCCAAATGTTGTTTATACTCCTCTAGTCCCATCACAGCCCCATTATAACTTGAATCTGCAAAGCATCTCTGTTAATGGTCAAATGTTACCCATCAATCCAACTGTCTTTACAACAACCAATGGCCAAGGAACCATAGTTGACTCTGGCACTACTTTGGCATACCTTGCTGAGGAAGCTTTCAACTCTTTTGTTGTTGCTATCACGAATTTAGTTTCACAAACGACACAGCCTGTTCTTCTCAAGGGAAATCAGTGTTATATAACCTCCTCCAGCATCTCTGACACATTTCCTCAAGTAAGCTTAAACTTTGCCGGTGGCGCATCGCTTGTTTTGAGACCTCAGGACTATCTCATACAACAAAGCTCTGCTGGTGGAAATACTGTTTGGTGTGTTGGTTTCCAGAAAATCCCAGGTCAAGGGATTACAATTCTAGGAGACCTTGTTCTAAAAGACAAAATCTTCATTTATGATTTAGCGAATCAACGAATTGGATGGGCTAACTACGACTGTTCAATGTCAGTAAATGTTTCCACAGCTTCCAAGACTGGACAGAGTGAGTCTGTCAACGCAGGGCAGCTCAGCGGCAGTGGCTCCGTGGAGAATCAGCCTGACGGACTTATTCTACAGCTAAGCATTCTTGTATTGTTCGTTCAATTATCCATCTTCACCAGCTTCTTCCACTCA

Coding sequence (CDS)

ATGGCCGCGATTGTTCGTACCGCAGTGTCGGTGGCCGCCGCGGTGCTGATGCTTCAGGCGGCCACGGTTCTCTGCGGGTTTCCCGCCAAGCTGAGTCTGGAGAGGGCTTTTCCGACGAATCATGGGGTCGAAATGGCTCAACTCCGAGGCCGGGACCGGGCTAGACATGGTAGAATGTTGCAGTCTTCTGGTGGTGTCATTGATTTTCCTGTGGCCGGGACCTATGATCCATTTCTCGTTGGGCTTTATTACACTAAAGTGCAGCTAGGTAATCCTCCGAAGGATTTCTTTGTGCAGATCGATACTGGAAGTGATGTTTTGTGGGTTAGCTGCAACTCTTGCAATGGATGCCCAGAAACTAGTGGGCTCCAGATTCAGCTCAATTTCTTTGATCCTGCTAGCTCATCAACAGCTTCTCCAGTCTCTTGTTCAGACCAAATTTGCGCCCTTGGAGTTCAGTCCTCTGATTCTGCCTGTTTGGGCCAGAGCAATCAATGTGCTTATGTCTTCCAGTATGGAGATGGGAGTGGAACATCGGGCTTTTACGTTAGGGACATGATGCATCTCGATATAGTAGTTGAGAGTTCTATGACTACAAATTCTTCAGCTTCAGTTGTATTTGGGTGTAGTACATCACAGACTGGAGACTTGACTAAGTCGGACAGAGCAGTTGATGGAATCTTCGGGTTCGGGCAACATGACTTGTCTGTAATTTCGCAACTGTCTTCACGAGGAATAGCACCAAAAGTGTTCTCCCACTGCTTGAAAGGAGATGATAGTGGTGGGGGAATATTGGTCCTTGGCGAGATTGTGGAGCCAAATGTTGTTTATACTCCTCTAGTCCCATCACAGCCCCATTATAACTTGAATCTGCAAAGCATCTCTGTTAATGGTCAAATGTTACCCATCAATCCAACTGTCTTTACAACAACCAATGGCCAAGGAACCATAGTTGACTCTGGCACTACTTTGGCATACCTTGCTGAGGAAGCTTTCAACTCTTTTGTTGTTGCTATCACGAATTTAGTTTCACAAACGACACAGCCTGTTCTTCTCAAGGGAAATCAGTGTTATATAACCTCCTCCAGCATCTCTGACACATTTCCTCAAGTAAGCTTAAACTTTGCCGGTGGCGCATCGCTTGTTTTGAGACCTCAGGACTATCTCATACAACAAAGCTCTGCTGGTGGAAATACTGTTTGGTGTGTTGGTTTCCAGAAAATCCCAGGTCAAGGGATTACAATTCTAGGAGACCTTGTTCTAAAAGACAAAATCTTCATTTATGATTTAGCGAATCAACGAATTGGATGGGCTAACTACGACTGTTCAATGTCAGTAAATGTTTCCACAGCTTCCAAGACTGGACAGAGTGAGTCTGTCAACGCAGGGCAGCTCAGCGGCAGTGGCTCCGTGGAGAATCAGCCTGACGGACTTATTCTACAGCTAAGCATTCTTGTATTGTTCGTTCAATTATCCATCTTCACCAGCTTCTTCCACTCA

Protein sequence

MAAIVRTAVSVAAAVLMLQAATVLCGFPAKLSLERAFPTNHGVEMAQLRGRDRARHGRMLQSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSCNGCPETSGLQIQLNFFDPASSSTASPVSCSDQICALGVQSSDSACLGQSNQCAYVFQYGDGSGTSGFYVRDMMHLDIVVESSMTTNSSASVVFGCSTSQTGDLTKSDRAVDGIFGFGQHDLSVISQLSSRGIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGQMLPINPTVFTTTNGQGTIVDSGTTLAYLAEEAFNSFVVAITNLVSQTTQPVLLKGNQCYITSSSISDTFPQVSLNFAGGASLVLRPQDYLIQQSSAGGNTVWCVGFQKIPGQGITILGDLVLKDKIFIYDLANQRIGWANYDCSMSVNVSTASKTGQSESVNAGQLSGSGSVENQPDGLILQLSILVLFVQLSIFTSFFHS
Homology
BLAST of MS005129 vs. NCBI nr
Match: XP_022140699.1 (aspartic proteinase-like protein 2 [Momordica charantia])

HSP 1 Score: 974.9 bits (2519), Expect = 2.6e-280
Identity = 498/500 (99.60%), Postives = 498/500 (99.60%), Query Frame = 0

Query: 1   MAAIVRTAVSVAAAVLMLQAATVLCGFPAKLSLERAFPTNHGVEMAQLRGRDRARHGRML 60
           MAAIVRTAVSV AAVLMLQAATVLCGFPAKLSLERAFPTNHGVEMAQLRGRDRARHGRML
Sbjct: 1   MAAIVRTAVSVVAAVLMLQAATVLCGFPAKLSLERAFPTNHGVEMAQLRGRDRARHGRML 60

Query: 61  QSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSCNGCPET 120
           QSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSCNGCPET
Sbjct: 61  QSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSCNGCPET 120

Query: 121 SGLQIQLNFFDPASSSTASPVSCSDQICALGVQSSDSACLGQSNQCAYVFQYGDGSGTSG 180
           SGLQIQLNFFDPASSSTASPVSCSDQICALGVQSSDSACLGQSNQCAYVFQYGDGSGTSG
Sbjct: 121 SGLQIQLNFFDPASSSTASPVSCSDQICALGVQSSDSACLGQSNQCAYVFQYGDGSGTSG 180

Query: 181 FYVRDMMHLDIVVESSMTTNSSASVVFGCSTSQTGDLTKSDRAVDGIFGFGQHDLSVISQ 240
           FYVRDMMHLDIVVESSMTTNSSASVVFGCSTSQTGDLTKSDRAVDGIFGFGQHDLSVISQ
Sbjct: 181 FYVRDMMHLDIVVESSMTTNSSASVVFGCSTSQTGDLTKSDRAVDGIFGFGQHDLSVISQ 240

Query: 241 LSSRGIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGQM 300
           LSSRGIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGQM
Sbjct: 241 LSSRGIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGQM 300

Query: 301 LPINPTVFTTTNGQGTIVDSGTTLAYLAEEAFNSFVVAITNLVSQTTQPVLLKGNQCYIT 360
           LPINPTVFTTTNGQGTIVDSGTTLAYLAEEAFNSFVVAITNLVSQTTQPVLLKGNQCYIT
Sbjct: 301 LPINPTVFTTTNGQGTIVDSGTTLAYLAEEAFNSFVVAITNLVSQTTQPVLLKGNQCYIT 360

Query: 361 SSSISDTFPQVSLNFAGGASLVLRPQDYLIQQSSAGGNTVWCVGFQKIPGQGITILGDLV 420
           SSSISDTFPQVSLNFAGGASLVLRPQDYLIQQSSAGGNTVWCVGFQKIPGQGITILGDLV
Sbjct: 361 SSSISDTFPQVSLNFAGGASLVLRPQDYLIQQSSAGGNTVWCVGFQKIPGQGITILGDLV 420

Query: 421 LKDKIFIYDLANQRIGWANYDCSMSVNVSTASKTGQSESVNAGQLSGSGSVENQPDGLIL 480
           LKD IFIYDLANQRIGWANYDCSMSVNVSTASKTGQSESVNAGQLSGSGSVENQPDGLIL
Sbjct: 421 LKDXIFIYDLANQRIGWANYDCSMSVNVSTASKTGQSESVNAGQLSGSGSVENQPDGLIL 480

Query: 481 QLSILVLFVQLSIFTSFFHS 501
           QLSILVLFVQLSIFTSFFHS
Sbjct: 481 QLSILVLFVQLSIFTSFFHS 500

BLAST of MS005129 vs. NCBI nr
Match: XP_038902575.1 (aspartic proteinase 39 [Benincasa hispida])

HSP 1 Score: 882.5 bits (2279), Expect = 1.7e-252
Identity = 441/500 (88.20%), Postives = 471/500 (94.20%), Query Frame = 0

Query: 1   MAAIVRTAVSVAAAVLMLQAATVLCGFPAKLSLERAFPTNHGVEMAQLRGRDRARHGRML 60
           MA IVR  VSVA  V++ QA TVLCGFPAKL+LERAFPTNHGVE+A LRGRD+ARHGRML
Sbjct: 1   MAGIVRAGVSVAVLVVIAQAVTVLCGFPAKLTLERAFPTNHGVEIAHLRGRDQARHGRML 60

Query: 61  QSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSCNGCPET 120
           QSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDF+VQIDTGSDVLWVSCNSCNGCP T
Sbjct: 61  QSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFYVQIDTGSDVLWVSCNSCNGCPAT 120

Query: 121 SGLQIQLNFFDPASSSTASPVSCSDQICALGVQSSDSACLGQSNQCAYVFQYGDGSGTSG 180
           SGLQIQLNFFDP SS+TAS VSCSDQICALGVQSSDSAC GQSNQCAYVFQYGDGSGTSG
Sbjct: 121 SGLQIQLNFFDPGSSTTASLVSCSDQICALGVQSSDSACFGQSNQCAYVFQYGDGSGTSG 180

Query: 181 FYVRDMMHLDIVVESSMTTNSSASVVFGCSTSQTGDLTKSDRAVDGIFGFGQHDLSVISQ 240
           +YV DM+HLDIVV+S+ T+NSSASV+FGCSTSQTGDLTKSDRAVDGIFGFGQ DLSVISQ
Sbjct: 181 YYVMDMIHLDIVVDSTATSNSSASVMFGCSTSQTGDLTKSDRAVDGIFGFGQQDLSVISQ 240

Query: 241 LSSRGIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGQM 300
           LSSRGIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGQ+
Sbjct: 241 LSSRGIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGQV 300

Query: 301 LPINPTVFTTTNGQGTIVDSGTTLAYLAEEAFNSFVVAITNLVSQTTQPVLLKGNQCYIT 360
           LPI+P VF T+N QGTI+DSGTTLAYLAEEA+NSFVVA+TN+VSQ+TQ V+LKGNQCY+T
Sbjct: 301 LPISPAVFATSNSQGTIIDSGTTLAYLAEEAYNSFVVAVTNIVSQSTQSVVLKGNQCYVT 360

Query: 361 SSSISDTFPQVSLNFAGGASLVLRPQDYLIQQSSAGGNTVWCVGFQKIPGQGITILGDLV 420
           SSS++D FPQVSLNFAGGASLVLRPQDYLIQQ+S GG TVWCVGFQKIPGQGITILGDLV
Sbjct: 361 SSSVADIFPQVSLNFAGGASLVLRPQDYLIQQNSVGGTTVWCVGFQKIPGQGITILGDLV 420

Query: 421 LKDKIFIYDLANQRIGWANYDCSMSVNVSTASKTGQSESVNAGQLSGSGSVENQPDGLIL 480
           LKDKIFIYDLANQRIGW NYDCSMSVNVSTA+KTG+SE VNAGQ S +GSV+NQPD  IL
Sbjct: 421 LKDKIFIYDLANQRIGWTNYDCSMSVNVSTATKTGKSEFVNAGQFSDNGSVQNQPDRFIL 480

Query: 481 QLSILVLFVQLSIFTSFFHS 501
            LSILVLFVQLSIFTSFFHS
Sbjct: 481 NLSILVLFVQLSIFTSFFHS 500

BLAST of MS005129 vs. NCBI nr
Match: XP_022995311.1 (aspartic proteinase-like protein 2 [Cucurbita maxima])

HSP 1 Score: 872.8 bits (2254), Expect = 1.4e-249
Identity = 439/499 (87.98%), Postives = 472/499 (94.59%), Query Frame = 0

Query: 1   MAAIVRTAVSVAAAVLMLQAATVLCGFPAKLSLERAFPTNHGVEMAQLRGRDRARHGRML 60
           MAAIVRT VSV  AV+M+Q ATVLCGFPAKL+LERAFPTNHGVE+AQLRGRDR RHGR+L
Sbjct: 1   MAAIVRTGVSVVVAVMMIQVATVLCGFPAKLTLERAFPTNHGVELAQLRGRDRIRHGRIL 60

Query: 61  QSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSCNGCPET 120
           QSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSC+GCPET
Sbjct: 61  QSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSCSGCPET 120

Query: 121 SGLQIQLNFFDPASSSTASPVSCSDQICALGVQSSDSACLGQSNQCAYVFQYGDGSGTSG 180
           SGLQIQLNFFDP SSSTAS VSCSDQICA+GVQSSDSACLGQSNQCAYVFQYGDGSGTSG
Sbjct: 121 SGLQIQLNFFDPGSSSTASLVSCSDQICAVGVQSSDSACLGQSNQCAYVFQYGDGSGTSG 180

Query: 181 FYVRDMMHLDIVVESSMTTNSSASVVFGCSTSQTGDLTKSDRAVDGIFGFGQHDLSVISQ 240
           +YV DM+HLDIVV+S+MTTNSSASV+FGCSTSQTGDLTKSDRA+DGIFGFGQ DLSVISQ
Sbjct: 181 YYVMDMIHLDIVVDSAMTTNSSASVMFGCSTSQTGDLTKSDRAIDGIFGFGQQDLSVISQ 240

Query: 241 LSSRGIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGQM 300
           LSSRG+APKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNG++
Sbjct: 241 LSSRGLAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGRV 300

Query: 301 LPINPTVFTTTNGQGTIVDSGTTLAYLAEEAFNSFVVAITNLVSQTTQPVLLKGNQCYIT 360
           LPINP VF T+N QGTI+DSGTTLAYLAEEA+++FV AITN VSQ+TQ ++L+GNQCY+T
Sbjct: 301 LPINPAVFATSNSQGTIIDSGTTLAYLAEEAYDTFVAAITNTVSQSTQSIVLRGNQCYMT 360

Query: 361 SSSISDTFPQVSLNFAGGASLVLRPQDYLIQQSSAGGNTVWCVGFQKIPGQGITILGDLV 420
           SSSISD FP VSLNFAGGASLVLRPQDYLIQQSS  G TVWCVGFQKIPGQGITILGDLV
Sbjct: 361 SSSISDIFPLVSLNFAGGASLVLRPQDYLIQQSSVSGTTVWCVGFQKIPGQGITILGDLV 420

Query: 421 LKDKIFIYDLANQRIGWANYDCSMSVNVSTASKTGQSESVNAGQLSGSGSVENQPDGLIL 480
           LKDKIFIYDLANQRIGWANYDCS SVNVSTA+KTG+SE VNAGQLS SGSV+NQP+ +IL
Sbjct: 421 LKDKIFIYDLANQRIGWANYDCSTSVNVSTATKTGKSEFVNAGQLSDSGSVQNQPNRVIL 480

Query: 481 QLSILVLFVQLSIFTSFFH 500
            LSILVLFV+LSIF+ F H
Sbjct: 481 -LSILVLFVKLSIFSGFLH 498

BLAST of MS005129 vs. NCBI nr
Match: KAG6606020.1 (Aspartic proteinase-like protein 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035970.1 Aspartic proteinase-like protein 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 871.7 bits (2251), Expect = 3.1e-249
Identity = 438/499 (87.78%), Postives = 473/499 (94.79%), Query Frame = 0

Query: 1   MAAIVRTAVSVAAAVLMLQAATVLCGFPAKLSLERAFPTNHGVEMAQLRGRDRARHGRML 60
           M AIVRT VSVA AV+M+QAATVLCGFPAKL+LERAFPTNHGVE+AQLRGRDR RHGR+L
Sbjct: 1   MVAIVRTGVSVAVAVVMIQAATVLCGFPAKLTLERAFPTNHGVELAQLRGRDRIRHGRIL 60

Query: 61  QSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSCNGCPET 120
           QSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSC+GCPET
Sbjct: 61  QSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSCSGCPET 120

Query: 121 SGLQIQLNFFDPASSSTASPVSCSDQICALGVQSSDSACLGQSNQCAYVFQYGDGSGTSG 180
           SGLQIQLNFFDP SSSTAS VSCSDQICA+GVQSSDSACLGQSNQCAYVFQYGDGSGTSG
Sbjct: 121 SGLQIQLNFFDPGSSSTASLVSCSDQICAVGVQSSDSACLGQSNQCAYVFQYGDGSGTSG 180

Query: 181 FYVRDMMHLDIVVESSMTTNSSASVVFGCSTSQTGDLTKSDRAVDGIFGFGQHDLSVISQ 240
           +YV DM+HLDIVV+S+MTTNSSASV+FGCSTSQTGDLTKSDRA+DGIFGFGQ DLSVISQ
Sbjct: 181 YYVMDMIHLDIVVDSAMTTNSSASVMFGCSTSQTGDLTKSDRAIDGIFGFGQQDLSVISQ 240

Query: 241 LSSRGIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGQM 300
           LSSRG+APKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNG++
Sbjct: 241 LSSRGLAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGRV 300

Query: 301 LPINPTVFTTTNGQGTIVDSGTTLAYLAEEAFNSFVVAITNLVSQTTQPVLLKGNQCYIT 360
           LPINP VF T+N QGTI+DSGTTLAYLAEEA+++FV AITN VSQ++Q ++L+GNQCY+T
Sbjct: 301 LPINPAVFATSNSQGTIIDSGTTLAYLAEEAYDTFVAAITNTVSQSSQSIILRGNQCYMT 360

Query: 361 SSSISDTFPQVSLNFAGGASLVLRPQDYLIQQSSAGGNTVWCVGFQKIPGQGITILGDLV 420
           SSSISD FP VSLNFAGGASLVLRPQDYLIQQSS  G TVWCVGFQKIPGQGITILGDLV
Sbjct: 361 SSSISDIFPLVSLNFAGGASLVLRPQDYLIQQSSVSGTTVWCVGFQKIPGQGITILGDLV 420

Query: 421 LKDKIFIYDLANQRIGWANYDCSMSVNVSTASKTGQSESVNAGQLSGSGSVENQPDGLIL 480
           LKDKIFIYDLANQRIGWANYDCS SVNVSTA+KTG+SE VNAGQLS SGSV+NQP+ +I+
Sbjct: 421 LKDKIFIYDLANQRIGWANYDCSTSVNVSTATKTGKSEFVNAGQLSDSGSVQNQPNRVIV 480

Query: 481 QLSILVLFVQLSIFTSFFH 500
            LSILVLFV+LSIF+ F H
Sbjct: 481 -LSILVLFVKLSIFSGFLH 498

BLAST of MS005129 vs. NCBI nr
Match: XP_022958429.1 (aspartic proteinase-like protein 2 [Cucurbita moschata])

HSP 1 Score: 870.2 bits (2247), Expect = 8.9e-249
Identity = 437/499 (87.58%), Postives = 472/499 (94.59%), Query Frame = 0

Query: 1   MAAIVRTAVSVAAAVLMLQAATVLCGFPAKLSLERAFPTNHGVEMAQLRGRDRARHGRML 60
           M AIVRT VSVA AV+M+QAATVLCGFPAKL+LERAFPTNHGVE+AQLRGRDR RHGR+L
Sbjct: 1   MVAIVRTGVSVAVAVVMIQAATVLCGFPAKLTLERAFPTNHGVELAQLRGRDRIRHGRIL 60

Query: 61  QSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSCNGCPET 120
           QSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSC+GCPET
Sbjct: 61  QSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSCSGCPET 120

Query: 121 SGLQIQLNFFDPASSSTASPVSCSDQICALGVQSSDSACLGQSNQCAYVFQYGDGSGTSG 180
           SGLQIQLNFFDP SSSTAS VSCSDQICA+GVQSSDSACLGQSNQCAYVFQYGDGSGTSG
Sbjct: 121 SGLQIQLNFFDPGSSSTASLVSCSDQICAVGVQSSDSACLGQSNQCAYVFQYGDGSGTSG 180

Query: 181 FYVRDMMHLDIVVESSMTTNSSASVVFGCSTSQTGDLTKSDRAVDGIFGFGQHDLSVISQ 240
           +YV DM+HLDIVV+S+MTTNSSASV+FGCSTSQTGDLTKSDRA+DGIFGFGQ DLSVISQ
Sbjct: 181 YYVMDMIHLDIVVDSAMTTNSSASVMFGCSTSQTGDLTKSDRAIDGIFGFGQQDLSVISQ 240

Query: 241 LSSRGIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGQM 300
           LSSRG+APKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNG++
Sbjct: 241 LSSRGLAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGRV 300

Query: 301 LPINPTVFTTTNGQGTIVDSGTTLAYLAEEAFNSFVVAITNLVSQTTQPVLLKGNQCYIT 360
           LPINP VF T+N QGTI+DSGTTLAYLAEEA+++FV AITN VSQ++Q ++L+GNQCY+T
Sbjct: 301 LPINPAVFATSNSQGTIIDSGTTLAYLAEEAYDTFVAAITNTVSQSSQSIILRGNQCYMT 360

Query: 361 SSSISDTFPQVSLNFAGGASLVLRPQDYLIQQSSAGGNTVWCVGFQKIPGQGITILGDLV 420
           SSSISD FP VSLNFAGGASLVLRPQDYLIQQSS  G TVWCVGFQKIPGQGITILGDLV
Sbjct: 361 SSSISDIFPLVSLNFAGGASLVLRPQDYLIQQSSVSGTTVWCVGFQKIPGQGITILGDLV 420

Query: 421 LKDKIFIYDLANQRIGWANYDCSMSVNVSTASKTGQSESVNAGQLSGSGSVENQPDGLIL 480
           LKDKIFIYDLANQRIGW NYDCS SVNVSTA+KTG+SE VNAGQLS SGSV+NQP+ +I+
Sbjct: 421 LKDKIFIYDLANQRIGWTNYDCSTSVNVSTATKTGKSEFVNAGQLSDSGSVQNQPNRVIV 480

Query: 481 QLSILVLFVQLSIFTSFFH 500
            LSILVLFV+LSIF+ F H
Sbjct: 481 -LSILVLFVKLSIFSGFLH 498

BLAST of MS005129 vs. ExPASy Swiss-Prot
Match: Q4V3D2 (Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 2.1e-83
Identity = 176/449 (39.20%), Postives = 266/449 (59.24%), Query Frame = 0

Query: 44  EMAQLRGRDRARHGRMLQSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTG 103
           ++++L+  D  RH RML +    ID P+ G      +GLY+TK++LG+PPK+++VQ+DTG
Sbjct: 43  QLSELKSHDSFRHARMLAN----IDLPLGGDSRADSIGLYFTKIKLGSPPKEYYVQVDTG 102

Query: 104 SDVLWVSCNSCNGCPETSGLQIQLNFFDPASSSTASPVSCSDQICALGVQSSDSACLGQS 163
           SD+LWV+C  C  CP  + L I L+ +D  +SST+  V C D  C+  +QS      G  
Sbjct: 103 SDILWVNCAPCPKCPVKTDLGIPLSLYDSKTSSTSKNVGCEDDFCSFIMQSE---TCGAK 162

Query: 164 NQCAYVFQYGDGSGTSGFYVRDMMHLDIVVESSMTTNSSASVVFGCSTSQTGDLTKSDRA 223
             C+Y   YGDGS + G +++D + L+ V  +  T   +  VVFGC  +Q+G L ++D A
Sbjct: 163 KPCSYHVVYGDGSTSDGDFIKDNITLEQVTGNLRTAPLAQEVVFGCGKNQSGQLGQTDSA 222

Query: 224 VDGIFGFGQHDLSVISQLSSRGIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPS 283
           VDGI GFGQ + S+ISQL++ G   ++FSHCL  + +GGGI  +GE+  P V  TP+VP+
Sbjct: 223 VDGIMGFGQSNTSIISQLAAGGSTKRIFSHCL-DNMNGGGIFAVGEVESPVVKTTPIVPN 282

Query: 284 QPHYNLNLQSISVNGQMLPINPTVFTTTNGQGTIVDSGTTLAYLAEEAFNSFVVAITNLV 343
           Q HYN+ L+ + V+G  + + P++ +T    GTI+DSGTTLAYL +  +NS +  IT   
Sbjct: 283 QVHYNVILKGMDVDGDPIDLPPSLASTNGDGGTIIDSGTTLAYLPQNLYNSLIEKIT-AK 342

Query: 344 SQTTQPVLLKGNQCYITSSSISDTFPQVSLNFAGGASLVLRPQDYLIQQSSAGGNTVWCV 403
            Q    ++ +   C+  +S+    FP V+L+F     L + P DYL          ++C 
Sbjct: 343 QQVKLHMVQETFACFSFTSNTDKAFPVVNLHFEDSLKLSVYPHDYLFSLR----EDMYCF 402

Query: 404 GFQK-----IPGQGITILGDLVLKDKIFIYDLANQRIGWANYDCSMSVNVSTASKTGQSE 463
           G+Q        G  + +LGDLVL +K+ +YDL N+ IGWA+++CS S+ V   S  G + 
Sbjct: 403 GWQSGGMTTQDGADVILLGDLVLSNKLVVYDLENEVIGWADHNCSSSIKVKDGS--GAAY 462

Query: 464 SVNAGQL-SGSGSVENQPDGLILQLSILV 487
            + A  L S + SV N    L+  LSIL+
Sbjct: 463 QLGAENLISAASSVMN--GTLVTLLSILI 474

BLAST of MS005129 vs. ExPASy Swiss-Prot
Match: Q9S9K4 (Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2)

HSP 1 Score: 290.8 bits (743), Expect = 2.9e-77
Identity = 175/438 (39.95%), Postives = 246/438 (56.16%), Query Frame = 0

Query: 45  MAQLRGRDRARHGRMLQSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGS 104
           +   +  D  RH RML S    ID P+ G      VGLY+TK++LG+PPK++ VQ+DTGS
Sbjct: 40  LEHFKSHDTRRHSRMLAS----IDLPLGGDSRVDSVGLYFTKIKLGSPPKEYHVQVDTGS 99

Query: 105 DVLWVSCNSCNGCPETSGLQIQLNFFDPASSSTASPVSCSDQICALGVQSSDSA--CLGQ 164
           D+LW++C  C  CP  + L  +L+ FD  +SST+  V C D  C+  +  SDS    LG 
Sbjct: 100 DILWINCKPCPKCPTKTNLNFRLSLFDMNASSTSKKVGCDDDFCSF-ISQSDSCQPALG- 159

Query: 165 SNQCAYVFQYGDGSGTSGFYVRDMMHLDIVVESSMTTNSSASVVFGCSTSQTGDLTKSDR 224
              C+Y   Y D S + G ++RDM+ L+ V     T      VVFGC + Q+G L   D 
Sbjct: 160 ---CSYHIVYADESTSDGKFIRDMLTLEQVTGDLKTGPLGQEVVFGCGSDQSGQLGNGDS 219

Query: 225 AVDGIFGFGQHDLSVISQLSSRGIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVP 284
           AVDG+ GFGQ + SV+SQL++ G A +VFSHCL  +  GGGI  +G +  P V  TP+VP
Sbjct: 220 AVDGVMGFGQSNTSVLSQLAATGDAKRVFSHCL-DNVKGGGIFAVGVVDSPKVKTTPMVP 279

Query: 285 SQPHYNLNLQSISVNGQMLPINPTVFTTTNGQGTIVDSGTTLAYLAEEAFNSFVVAITNL 344
           +Q HYN+ L  + V+G  L +  ++    NG GTIVDSGTTLAY  +  ++S +  I   
Sbjct: 280 NQMHYNVMLMGMDVDGTSLDLPRSI--VRNG-GTIVDSGTTLAYFPKVLYDSLIETIL-- 339

Query: 345 VSQTTQPVLL----KGNQCYITSSSISDTFPQVSLNFAGGASLVLRPQDYLIQQSSAGGN 404
                QPV L    +  QC+  S+++ + FP VS  F     L + P DYL         
Sbjct: 340 ---ARQPVKLHIVEETFQCFSFSTNVDEAFPPVSFEFEDSVKLTVYPHDYLFTLE----E 399

Query: 405 TVWCVGFQKIPGQGIT--------ILGDLVLKDKIFIYDLANQRIGWANYDCSMSVNVST 464
            ++C G+Q     G+T        +LGDLVL +K+ +YDL N+ IGWA+++CS S+ +  
Sbjct: 400 ELYCFGWQ---AGGLTTDERSEVILLGDLVLSNKLVVYDLDNEVIGWADHNCSSSIKIKD 450

Query: 465 ASKTGQSESVNAGQLSGS 469
            S  G   SV A  LS +
Sbjct: 460 GS--GGVYSVGADNLSSA 450

BLAST of MS005129 vs. ExPASy Swiss-Prot
Match: Q8VYV9 (Aspartyl protease family protein 1 OS=Arabidopsis thaliana OX=3702 GN=APF1 PE=2 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 1.5e-33
Identity = 113/373 (30.29%), Postives = 168/373 (45.04%), Query Frame = 0

Query: 82  LYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSCNGC----PETSGLQIQLNFFDPASSST 141
           L+Y  V +G P   F V +DTGSD+ W+ C+ C  C        G  + LN + P +SST
Sbjct: 103 LHYANVTVGTPSDWFMVALDTGSDLFWLPCD-CTNCVRELKAPGGSSLDLNIYSPNASST 162

Query: 142 ASPVSCSDQICALGVQSSDSACLGQSNQCAYVFQY-GDGSGTSGFYVRDMMHLDIVVESS 201
           ++ V C+  +C  G       C    + C Y  +Y  +G+ ++G  V D++HL  V    
Sbjct: 163 STKVPCNSTLCTRG-----DRCASPESDCPYQIRYLSNGTSSTGVLVEDVLHL--VSNDK 222

Query: 202 MTTNSSASVVFGCSTSQTGDLTKSDRAVDGIFGFGQHDLSVISQLSSRGIAPKVFSHCLK 261
            +    A V FGC   QTG +     A +G+FG G  D+SV S L+  GIA   FS C  
Sbjct: 223 SSKAIPARVTFGCGQVQTG-VFHDGAAPNGLFGLGLEDISVPSVLAKEGIAANSFSMCFG 282

Query: 262 GDDSGGGILVLGEIVEPNVVYTPLVPSQPH--YNLNLQSISVNGQMLPINPTVFTTTNGQ 321
            D  G G +  G+    +   TPL   QPH  YN+ +  ISV G          T     
Sbjct: 283 ND--GAGRISFGDKGSVDQRETPLNIRQPHPTYNITVTKISVGGN---------TGDLEF 342

Query: 322 GTIVDSGTTLAYLAEEAFNSFVVAITNLV----SQTTQPVLLKGNQCY-ITSSSISDTFP 381
             + DSGT+  YL + A+     +  +L      QTT    L    CY ++ +  S  +P
Sbjct: 343 DAVFDSGTSFTYLTDAAYTLISESFNSLALDKRYQTTDSE-LPFEYCYALSPNKDSFQYP 402

Query: 382 QVSLNFAGGASLVLRPQDYLIQQSSAGGNTVWCVGFQKIPGQGITILGDLVLKDKIFIYD 441
            V+L   GG+S  +     +I         V+C+   KI  + I+I+G   +     ++D
Sbjct: 403 AVNLTMKGGSSYPVYHPLVVIPMKD---TDVYCLAIMKI--EDISIIGQNFMTGYRVVFD 449

Query: 442 LANQRIGWANYDC 443
                +GW   DC
Sbjct: 463 REKLILGWKESDC 449

BLAST of MS005129 vs. ExPASy Swiss-Prot
Match: Q9LX20 (Aspartic proteinase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g10080 PE=2 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 2.9e-32
Identity = 111/394 (28.17%), Postives = 174/394 (44.16%), Query Frame = 0

Query: 82  LYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSCNGCPETSGLQI-----QLNFFDPASSS 141
           L+YT + +G P   F V +DTGS++LW+ CN     P TS          LN ++P+SSS
Sbjct: 99  LHYTWIDIGTPSVSFLVALDTGSNLLWIPCNCVQCAPLTSTYYSSLATKDLNEYNPSSSS 158

Query: 142 TASPVSCSDQICALGVQSSDSACLGQSNQCAYVFQYGDG-SGTSGFYVRDMMHLDIVVES 201
           T+    CS ++C      S S C     QC Y   Y  G + +SG  V D++HL     +
Sbjct: 159 TSKVFLCSHKLC-----DSASDCESPKEQCPYTVNYLSGNTSSSGLLVEDILHLTYNTNN 218

Query: 202 SMTTNSS---ASVVFGCSTSQTGDLTKSDRAVDGIFGFGQHDLSVISQLSSRGIAPKVFS 261
            +   SS   A VV GC   Q+GD      A DG+ G G  ++SV S LS  G+    FS
Sbjct: 219 RLMNGSSSVKARVVIGCGKKQSGDYL-DGVAPDGLMGLGPAEISVPSFLSKAGLMRNSFS 278

Query: 262 HCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPH----YNLNLQSISVNGQMLPINPTVF 321
            C   +DSG   +  G++       TP +    +    Y + +++  +    L    T F
Sbjct: 279 LCFDEEDSGR--IYFGDMGPSIQQSTPFLQLDNNKYSGYIVGVEACCIGNSCL--KQTSF 338

Query: 322 TTTNGQGTIVDSGTTLAYLAEEAFNSFVVAITNLVSQTTQPVL-LKGNQCYITSSSISDT 381
           T      T +DSG +  YL EE +    + I   ++ T++    +    CY   SS    
Sbjct: 339 T------TFIDSGQSFTYLPEEIYRKVALEIDRHINATSKNFEGVSWEYCY--ESSAEPK 398

Query: 382 FPQVSLNFAGGASLVLRPQDYLIQQSSAGGNTVWCVGFQKIPGQGITILGDLVLKDKIFI 441
            P + L F+   + V+    ++ QQS   G   +C+       +GI  +G   ++    +
Sbjct: 399 VPAIKLKFSHNNTFVIHKPLFVFQQSQ--GLVQFCLPISPSGQEGIGSIGQNYMRGYRMV 458

Query: 442 YDLANQRIGWANYDCSMSVNVSTASKTGQSESVN 462
           +D  N ++GW+   C         +  G + S N
Sbjct: 459 FDRENMKLGWSPSKCQEDKIEPPQASPGSTSSPN 472

BLAST of MS005129 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 9.2e-31
Identity = 125/417 (29.98%), Postives = 184/417 (44.12%), Query Frame = 0

Query: 43  VEMAQLRGRDRARH-GRMLQSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQID 102
           ++ A  RG  R R    MLQSS G+     AG       G Y   V +G P   F   +D
Sbjct: 61  IKRAIKRGERRMRSINAMLQSSSGIETPVYAGD------GEYLMNVAIGTPDSSFSAIMD 120

Query: 103 TGSDVLWVSCNSCNGCPETSGLQIQLNFFDPASSSTASPVSCSDQICALGVQSSDSACLG 162
           TGSD++W  C  C  C            F+P  SS+ S + C  Q C          C  
Sbjct: 121 TGSDLIWTQCEPCTQC-----FSQPTPIFNPQDSSSFSTLPCESQYCQ---DLPSETC-- 180

Query: 163 QSNQCAYVFQYGDGSGTSGFYVRDMMHLDIVVESSMTTNSSASVVFGCSTSQTGDLTKSD 222
            +N+C Y + YGDGS T G+   +          +  T+S  ++ FGC     G   + +
Sbjct: 181 NNNECQYTYGYGDGSTTQGYMATETF--------TFETSSVPNIAFGCGEDNQG-FGQGN 240

Query: 223 RAVDGIFGFGQHDLSVISQLSSRGIAPKVFSHCLKG-DDSGGGILVLGEIV--------E 282
            A  G+ G G   LS+ SQL   G+    FS+C+     S    L LG            
Sbjct: 241 GA--GLIGMGWGPLSLPSQL---GVGQ--FSYCMTSYGSSSPSTLALGSAASGVPEGSPS 300

Query: 283 PNVVYTPLVPSQPHYNLNLQSISVNGQMLPINPTVFTTTNG--QGTIVDSGTTLAYLAEE 342
             ++++ L P+  +Y + LQ I+V G  L I  + F   +    G I+DSGTTL YL ++
Sbjct: 301 TTLIHSSLNPT--YYYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQD 360

Query: 343 AFNSFVVAITNLVSQTTQPVLLKG-NQCYITSSSISDT-FPQVSLNFAGGASLVLRPQDY 402
           A+N+   A T+ ++  T      G + C+   S  S    P++S+ F GG  L L  Q+ 
Sbjct: 361 AYNAVAQAFTDQINLPTVDESSSGLSTCFQQPSDGSTVQVPEISMQFDGGV-LNLGEQNI 420

Query: 403 LIQQSSAGGNTVWCVGFQKIPGQGITILGDLVLKDKIFIYDLANQRIGWANYDCSMS 446
           LI  S A G  V C+        GI+I G++  ++   +YDL N  + +    C  S
Sbjct: 421 LI--SPAEG--VICLAMGSSSQLGISIFGNIQQQETQVLYDLQNLAVSFVPTQCGAS 438

BLAST of MS005129 vs. ExPASy TrEMBL
Match: A0A6J1CGE5 (aspartic proteinase-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111011299 PE=3 SV=1)

HSP 1 Score: 974.9 bits (2519), Expect = 1.2e-280
Identity = 498/500 (99.60%), Postives = 498/500 (99.60%), Query Frame = 0

Query: 1   MAAIVRTAVSVAAAVLMLQAATVLCGFPAKLSLERAFPTNHGVEMAQLRGRDRARHGRML 60
           MAAIVRTAVSV AAVLMLQAATVLCGFPAKLSLERAFPTNHGVEMAQLRGRDRARHGRML
Sbjct: 1   MAAIVRTAVSVVAAVLMLQAATVLCGFPAKLSLERAFPTNHGVEMAQLRGRDRARHGRML 60

Query: 61  QSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSCNGCPET 120
           QSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSCNGCPET
Sbjct: 61  QSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSCNGCPET 120

Query: 121 SGLQIQLNFFDPASSSTASPVSCSDQICALGVQSSDSACLGQSNQCAYVFQYGDGSGTSG 180
           SGLQIQLNFFDPASSSTASPVSCSDQICALGVQSSDSACLGQSNQCAYVFQYGDGSGTSG
Sbjct: 121 SGLQIQLNFFDPASSSTASPVSCSDQICALGVQSSDSACLGQSNQCAYVFQYGDGSGTSG 180

Query: 181 FYVRDMMHLDIVVESSMTTNSSASVVFGCSTSQTGDLTKSDRAVDGIFGFGQHDLSVISQ 240
           FYVRDMMHLDIVVESSMTTNSSASVVFGCSTSQTGDLTKSDRAVDGIFGFGQHDLSVISQ
Sbjct: 181 FYVRDMMHLDIVVESSMTTNSSASVVFGCSTSQTGDLTKSDRAVDGIFGFGQHDLSVISQ 240

Query: 241 LSSRGIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGQM 300
           LSSRGIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGQM
Sbjct: 241 LSSRGIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGQM 300

Query: 301 LPINPTVFTTTNGQGTIVDSGTTLAYLAEEAFNSFVVAITNLVSQTTQPVLLKGNQCYIT 360
           LPINPTVFTTTNGQGTIVDSGTTLAYLAEEAFNSFVVAITNLVSQTTQPVLLKGNQCYIT
Sbjct: 301 LPINPTVFTTTNGQGTIVDSGTTLAYLAEEAFNSFVVAITNLVSQTTQPVLLKGNQCYIT 360

Query: 361 SSSISDTFPQVSLNFAGGASLVLRPQDYLIQQSSAGGNTVWCVGFQKIPGQGITILGDLV 420
           SSSISDTFPQVSLNFAGGASLVLRPQDYLIQQSSAGGNTVWCVGFQKIPGQGITILGDLV
Sbjct: 361 SSSISDTFPQVSLNFAGGASLVLRPQDYLIQQSSAGGNTVWCVGFQKIPGQGITILGDLV 420

Query: 421 LKDKIFIYDLANQRIGWANYDCSMSVNVSTASKTGQSESVNAGQLSGSGSVENQPDGLIL 480
           LKD IFIYDLANQRIGWANYDCSMSVNVSTASKTGQSESVNAGQLSGSGSVENQPDGLIL
Sbjct: 421 LKDXIFIYDLANQRIGWANYDCSMSVNVSTASKTGQSESVNAGQLSGSGSVENQPDGLIL 480

Query: 481 QLSILVLFVQLSIFTSFFHS 501
           QLSILVLFVQLSIFTSFFHS
Sbjct: 481 QLSILVLFVQLSIFTSFFHS 500

BLAST of MS005129 vs. ExPASy TrEMBL
Match: A0A6J1K1L8 (aspartic proteinase-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111490894 PE=3 SV=1)

HSP 1 Score: 872.8 bits (2254), Expect = 6.6e-250
Identity = 439/499 (87.98%), Postives = 472/499 (94.59%), Query Frame = 0

Query: 1   MAAIVRTAVSVAAAVLMLQAATVLCGFPAKLSLERAFPTNHGVEMAQLRGRDRARHGRML 60
           MAAIVRT VSV  AV+M+Q ATVLCGFPAKL+LERAFPTNHGVE+AQLRGRDR RHGR+L
Sbjct: 1   MAAIVRTGVSVVVAVMMIQVATVLCGFPAKLTLERAFPTNHGVELAQLRGRDRIRHGRIL 60

Query: 61  QSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSCNGCPET 120
           QSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSC+GCPET
Sbjct: 61  QSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSCSGCPET 120

Query: 121 SGLQIQLNFFDPASSSTASPVSCSDQICALGVQSSDSACLGQSNQCAYVFQYGDGSGTSG 180
           SGLQIQLNFFDP SSSTAS VSCSDQICA+GVQSSDSACLGQSNQCAYVFQYGDGSGTSG
Sbjct: 121 SGLQIQLNFFDPGSSSTASLVSCSDQICAVGVQSSDSACLGQSNQCAYVFQYGDGSGTSG 180

Query: 181 FYVRDMMHLDIVVESSMTTNSSASVVFGCSTSQTGDLTKSDRAVDGIFGFGQHDLSVISQ 240
           +YV DM+HLDIVV+S+MTTNSSASV+FGCSTSQTGDLTKSDRA+DGIFGFGQ DLSVISQ
Sbjct: 181 YYVMDMIHLDIVVDSAMTTNSSASVMFGCSTSQTGDLTKSDRAIDGIFGFGQQDLSVISQ 240

Query: 241 LSSRGIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGQM 300
           LSSRG+APKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNG++
Sbjct: 241 LSSRGLAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGRV 300

Query: 301 LPINPTVFTTTNGQGTIVDSGTTLAYLAEEAFNSFVVAITNLVSQTTQPVLLKGNQCYIT 360
           LPINP VF T+N QGTI+DSGTTLAYLAEEA+++FV AITN VSQ+TQ ++L+GNQCY+T
Sbjct: 301 LPINPAVFATSNSQGTIIDSGTTLAYLAEEAYDTFVAAITNTVSQSTQSIVLRGNQCYMT 360

Query: 361 SSSISDTFPQVSLNFAGGASLVLRPQDYLIQQSSAGGNTVWCVGFQKIPGQGITILGDLV 420
           SSSISD FP VSLNFAGGASLVLRPQDYLIQQSS  G TVWCVGFQKIPGQGITILGDLV
Sbjct: 361 SSSISDIFPLVSLNFAGGASLVLRPQDYLIQQSSVSGTTVWCVGFQKIPGQGITILGDLV 420

Query: 421 LKDKIFIYDLANQRIGWANYDCSMSVNVSTASKTGQSESVNAGQLSGSGSVENQPDGLIL 480
           LKDKIFIYDLANQRIGWANYDCS SVNVSTA+KTG+SE VNAGQLS SGSV+NQP+ +IL
Sbjct: 421 LKDKIFIYDLANQRIGWANYDCSTSVNVSTATKTGKSEFVNAGQLSDSGSVQNQPNRVIL 480

Query: 481 QLSILVLFVQLSIFTSFFH 500
            LSILVLFV+LSIF+ F H
Sbjct: 481 -LSILVLFVKLSIFSGFLH 498

BLAST of MS005129 vs. ExPASy TrEMBL
Match: A0A6J1H528 (aspartic proteinase-like protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111459652 PE=3 SV=1)

HSP 1 Score: 870.2 bits (2247), Expect = 4.3e-249
Identity = 437/499 (87.58%), Postives = 472/499 (94.59%), Query Frame = 0

Query: 1   MAAIVRTAVSVAAAVLMLQAATVLCGFPAKLSLERAFPTNHGVEMAQLRGRDRARHGRML 60
           M AIVRT VSVA AV+M+QAATVLCGFPAKL+LERAFPTNHGVE+AQLRGRDR RHGR+L
Sbjct: 1   MVAIVRTGVSVAVAVVMIQAATVLCGFPAKLTLERAFPTNHGVELAQLRGRDRIRHGRIL 60

Query: 61  QSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSCNGCPET 120
           QSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSC+GCPET
Sbjct: 61  QSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSCSGCPET 120

Query: 121 SGLQIQLNFFDPASSSTASPVSCSDQICALGVQSSDSACLGQSNQCAYVFQYGDGSGTSG 180
           SGLQIQLNFFDP SSSTAS VSCSDQICA+GVQSSDSACLGQSNQCAYVFQYGDGSGTSG
Sbjct: 121 SGLQIQLNFFDPGSSSTASLVSCSDQICAVGVQSSDSACLGQSNQCAYVFQYGDGSGTSG 180

Query: 181 FYVRDMMHLDIVVESSMTTNSSASVVFGCSTSQTGDLTKSDRAVDGIFGFGQHDLSVISQ 240
           +YV DM+HLDIVV+S+MTTNSSASV+FGCSTSQTGDLTKSDRA+DGIFGFGQ DLSVISQ
Sbjct: 181 YYVMDMIHLDIVVDSAMTTNSSASVMFGCSTSQTGDLTKSDRAIDGIFGFGQQDLSVISQ 240

Query: 241 LSSRGIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGQM 300
           LSSRG+APKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNG++
Sbjct: 241 LSSRGLAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGRV 300

Query: 301 LPINPTVFTTTNGQGTIVDSGTTLAYLAEEAFNSFVVAITNLVSQTTQPVLLKGNQCYIT 360
           LPINP VF T+N QGTI+DSGTTLAYLAEEA+++FV AITN VSQ++Q ++L+GNQCY+T
Sbjct: 301 LPINPAVFATSNSQGTIIDSGTTLAYLAEEAYDTFVAAITNTVSQSSQSIILRGNQCYMT 360

Query: 361 SSSISDTFPQVSLNFAGGASLVLRPQDYLIQQSSAGGNTVWCVGFQKIPGQGITILGDLV 420
           SSSISD FP VSLNFAGGASLVLRPQDYLIQQSS  G TVWCVGFQKIPGQGITILGDLV
Sbjct: 361 SSSISDIFPLVSLNFAGGASLVLRPQDYLIQQSSVSGTTVWCVGFQKIPGQGITILGDLV 420

Query: 421 LKDKIFIYDLANQRIGWANYDCSMSVNVSTASKTGQSESVNAGQLSGSGSVENQPDGLIL 480
           LKDKIFIYDLANQRIGW NYDCS SVNVSTA+KTG+SE VNAGQLS SGSV+NQP+ +I+
Sbjct: 421 LKDKIFIYDLANQRIGWTNYDCSTSVNVSTATKTGKSEFVNAGQLSDSGSVQNQPNRVIV 480

Query: 481 QLSILVLFVQLSIFTSFFH 500
            LSILVLFV+LSIF+ F H
Sbjct: 481 -LSILVLFVKLSIFSGFLH 498

BLAST of MS005129 vs. ExPASy TrEMBL
Match: A0A5A7SVM3 (Aspartic proteinase-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold26G00060 PE=3 SV=1)

HSP 1 Score: 866.3 bits (2237), Expect = 6.2e-248
Identity = 431/500 (86.20%), Postives = 466/500 (93.20%), Query Frame = 0

Query: 1   MAAIVRTAVSVAAAVLMLQAATVLCGFPAKLSLERAFPTNHGVEMAQLRGRDRARHGRML 60
           M  IV   VSV   V+++QAATVLCGFPA L+LERAFPTNHGVE+A LRGRDR RHGRML
Sbjct: 1   MGRIVYAGVSVGVLVVLVQAATVLCGFPATLTLERAFPTNHGVEIAHLRGRDRVRHGRML 60

Query: 61  QSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSCNGCPET 120
           QSSGGVIDFPVAGTYDPFLVGLYYT+VQLGNPPKDF+VQIDTGSDVLWVSCNSCNGCP T
Sbjct: 61  QSSGGVIDFPVAGTYDPFLVGLYYTRVQLGNPPKDFYVQIDTGSDVLWVSCNSCNGCPAT 120

Query: 121 SGLQIQLNFFDPASSSTASPVSCSDQICALGVQSSDSACLGQSNQCAYVFQYGDGSGTSG 180
           SGLQIQLNFFDP SS+TAS VSCSDQICALGVQSSDSAC GQSNQCAYVFQYGDGSGTSG
Sbjct: 121 SGLQIQLNFFDPGSSTTASLVSCSDQICALGVQSSDSACFGQSNQCAYVFQYGDGSGTSG 180

Query: 181 FYVRDMMHLDIVVESSMTTNSSASVVFGCSTSQTGDLTKSDRAVDGIFGFGQHDLSVISQ 240
           +YV DM+HLD+VV+SS+T+NSSASV+FGCSTSQTGDLTKSDRAVDGIFGFGQ DLSVISQ
Sbjct: 181 YYVMDMIHLDVVVDSSVTSNSSASVMFGCSTSQTGDLTKSDRAVDGIFGFGQQDLSVISQ 240

Query: 241 LSSRGIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGQM 300
           LSSRGIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGQ+
Sbjct: 241 LSSRGIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGQV 300

Query: 301 LPINPTVFTTTNGQGTIVDSGTTLAYLAEEAFNSFVVAITNLVSQTTQPVLLKGNQCYIT 360
           LPI+P VF+T+N QGTI+DSGTTLAYLAEEA+N+FVVA+TN+VSQ+TQ V+LKGN+CY+T
Sbjct: 301 LPISPAVFSTSNSQGTIIDSGTTLAYLAEEAYNAFVVAVTNIVSQSTQSVVLKGNRCYVT 360

Query: 361 SSSISDTFPQVSLNFAGGASLVLRPQDYLIQQSSAGGNTVWCVGFQKIPGQGITILGDLV 420
           SSS+SD FPQVSLNFAGGASLVL  QDYLIQQ+S GG TVWC+GFQKIPGQGITILGDLV
Sbjct: 361 SSSVSDIFPQVSLNFAGGASLVLTAQDYLIQQNSVGGTTVWCIGFQKIPGQGITILGDLV 420

Query: 421 LKDKIFIYDLANQRIGWANYDCSMSVNVSTASKTGQSESVNAGQLSGSGSVENQPDGLIL 480
           LKDKIFIYDLANQRIGW NYDCSMSVNVSTA+KTG+SE VNAGQ S SGS++NQPD  IL
Sbjct: 421 LKDKIFIYDLANQRIGWTNYDCSMSVNVSTATKTGKSEYVNAGQFSDSGSMQNQPDRFIL 480

Query: 481 QLSILVLFVQLSIFTSFFHS 501
            LSI VLFVQL IFTSFFHS
Sbjct: 481 NLSIFVLFVQLYIFTSFFHS 500

BLAST of MS005129 vs. ExPASy TrEMBL
Match: A0A1S3C8H1 (aspartic proteinase-like protein 2 OS=Cucumis melo OX=3656 GN=LOC103497990 PE=3 SV=1)

HSP 1 Score: 866.3 bits (2237), Expect = 6.2e-248
Identity = 431/500 (86.20%), Postives = 466/500 (93.20%), Query Frame = 0

Query: 1   MAAIVRTAVSVAAAVLMLQAATVLCGFPAKLSLERAFPTNHGVEMAQLRGRDRARHGRML 60
           M  IV   VSV   V+++QAATVLCGFPA L+LERAFPTNHGVE+A LRGRDR RHGRML
Sbjct: 1   MGRIVYAGVSVGVLVVLVQAATVLCGFPATLTLERAFPTNHGVEIAHLRGRDRVRHGRML 60

Query: 61  QSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSCNGCPET 120
           QSSGGVIDFPVAGTYDPFLVGLYYT+VQLGNPPKDF+VQIDTGSDVLWVSCNSCNGCP T
Sbjct: 61  QSSGGVIDFPVAGTYDPFLVGLYYTRVQLGNPPKDFYVQIDTGSDVLWVSCNSCNGCPAT 120

Query: 121 SGLQIQLNFFDPASSSTASPVSCSDQICALGVQSSDSACLGQSNQCAYVFQYGDGSGTSG 180
           SGLQIQLNFFDP SS+TAS VSCSDQICALGVQSSDSAC GQSNQCAYVFQYGDGSGTSG
Sbjct: 121 SGLQIQLNFFDPGSSTTASLVSCSDQICALGVQSSDSACFGQSNQCAYVFQYGDGSGTSG 180

Query: 181 FYVRDMMHLDIVVESSMTTNSSASVVFGCSTSQTGDLTKSDRAVDGIFGFGQHDLSVISQ 240
           +YV DM+HLD+VV+SS+T+NSSASV+FGCSTSQTGDLTKSDRAVDGIFGFGQ DLSVISQ
Sbjct: 181 YYVMDMIHLDVVVDSSVTSNSSASVMFGCSTSQTGDLTKSDRAVDGIFGFGQQDLSVISQ 240

Query: 241 LSSRGIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGQM 300
           LSSRGIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGQ+
Sbjct: 241 LSSRGIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGQV 300

Query: 301 LPINPTVFTTTNGQGTIVDSGTTLAYLAEEAFNSFVVAITNLVSQTTQPVLLKGNQCYIT 360
           LPI+P VF+T+N QGTI+DSGTTLAYLAEEA+N+FVVA+TN+VSQ+TQ V+LKGN+CY+T
Sbjct: 301 LPISPAVFSTSNSQGTIIDSGTTLAYLAEEAYNAFVVAVTNIVSQSTQSVVLKGNRCYVT 360

Query: 361 SSSISDTFPQVSLNFAGGASLVLRPQDYLIQQSSAGGNTVWCVGFQKIPGQGITILGDLV 420
           SSS+SD FPQVSLNFAGGASLVL  QDYLIQQ+S GG TVWC+GFQKIPGQGITILGDLV
Sbjct: 361 SSSVSDIFPQVSLNFAGGASLVLTAQDYLIQQNSVGGTTVWCIGFQKIPGQGITILGDLV 420

Query: 421 LKDKIFIYDLANQRIGWANYDCSMSVNVSTASKTGQSESVNAGQLSGSGSVENQPDGLIL 480
           LKDKIFIYDLANQRIGW NYDCSMSVNVSTA+KTG+SE VNAGQ S SGS++NQPD  IL
Sbjct: 421 LKDKIFIYDLANQRIGWTNYDCSMSVNVSTATKTGKSEYVNAGQFSDSGSMQNQPDRFIL 480

Query: 481 QLSILVLFVQLSIFTSFFHS 501
            LSI VLFVQL IFTSFFHS
Sbjct: 481 NLSIFVLFVQLYIFTSFFHS 500

BLAST of MS005129 vs. TAIR 10
Match: AT5G22850.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 692.6 bits (1786), Expect = 2.4e-199
Identity = 338/490 (68.98%), Postives = 408/490 (83.27%), Query Frame = 0

Query: 8   AVSVAAAVL---MLQAATVLCGFPAKLSLERAFPTNHGVEMAQLRGRDRARHGRMLQSSG 67
           A+  AAA+L   +L AA +  GFPA L LER  P NH +E++QL+ RD ARHGR+LQS G
Sbjct: 3   AIRFAAAILICCLLPAAVLSYGFPAALKLERVIPANHEMELSQLKARDEARHGRLLQSLG 62

Query: 68  GVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSCNGCPETSGLQ 127
           GVIDFPV GT+DPF+VGLYYTK++LG PP+DF+VQ+DTGSDVLWVSC SCNGCP+TSGLQ
Sbjct: 63  GVIDFPVDGTFDPFVVGLYYTKLRLGTPPRDFYVQVDTGSDVLWVSCASCNGCPQTSGLQ 122

Query: 128 IQLNFFDPASSSTASPVSCSDQICALGVQSSDSACLGQSNQCAYVFQYGDGSGTSGFYVR 187
           IQLNFFDP SS TASP+SCSDQ C+ G+QSSDS C  Q+N CAY FQYGDGSGTSGFYV 
Sbjct: 123 IQLNFFDPGSSVTASPISCSDQRCSWGIQSSDSGCSVQNNLCAYTFQYGDGSGTSGFYVS 182

Query: 188 DMMHLDIVVESSMTTNSSASVVFGCSTSQTGDLTKSDRAVDGIFGFGQHDLSVISQLSSR 247
           D++  D++V SS+  NS+A VVFGCSTSQTGDL KSDRAVDGIFGFGQ  +SVISQL+S+
Sbjct: 183 DVLQFDMIVGSSLVPNSTAPVVFGCSTSQTGDLVKSDRAVDGIFGFGQQGMSVISQLASQ 242

Query: 248 GIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGQMLPIN 307
           GIAP+VFSHCLKG++ GGGILVLGEIVEPN+V+TPLVPSQPHYN+NL SISVNGQ LPIN
Sbjct: 243 GIAPRVFSHCLKGENGGGGILVLGEIVEPNMVFTPLVPSQPHYNVNLLSISVNGQALPIN 302

Query: 308 PTVFTTTNGQGTIVDSGTTLAYLAEEAFNSFVVAITNLVSQTTQPVLLKGNQCYITSSSI 367
           P+VF+T+NGQGTI+D+GTTLAYL+E A+  FV AITN VSQ+ +PV+ KGNQCY+ ++S+
Sbjct: 303 PSVFSTSNGQGTIIDTGTTLAYLSEAAYVPFVEAITNAVSQSVRPVVSKGNQCYVITTSV 362

Query: 368 SDTFPQVSLNFAGGASLVLRPQDYLIQQSSAGGNTVWCVGFQKIPGQGITILGDLVLKDK 427
            D FP VSLNFAGGAS+ L PQDYLIQQ++ GG  VWC+GFQ+I  QGITILGDLVLKDK
Sbjct: 363 GDIFPPVSLNFAGGASMFLNPQDYLIQQNNVGGTAVWCIGFQRIQNQGITILGDLVLKDK 422

Query: 428 IFIYDLANQRIGWANYDCSMSVNVSTASKTGQSESVNAGQLSGSGSVENQPDGLILQLSI 487
           IF+YDL  QRIGWANYDCS SVNVS  S +G+SE VNAGQ S + +   +    I+  ++
Sbjct: 423 IFVYDLVGQRIGWANYDCSTSVNVSATSSSGRSEYVNAGQFSENAAAPQKLSLDIVGNTL 482

Query: 488 LVLFVQLSIF 495
           ++L + +++F
Sbjct: 483 MLLLMVINMF 492

BLAST of MS005129 vs. TAIR 10
Match: AT1G08210.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 558.1 bits (1437), Expect = 7.0e-159
Identity = 291/489 (59.51%), Postives = 365/489 (74.64%), Query Frame = 0

Query: 1   MAAIVRTAVSVAAAVLMLQAATVLCGFPAKLSLERAFPTNHGVEMAQLRGRDRARHGRML 60
           MA      V + AAVL+L A T+ CG  A L LER  P NH + + +LR  D ARHGR+L
Sbjct: 1   MAVDSPAGVIIIAAVLLLAATTLACGSDAVLKLERLIPPNHELGLTELRAFDSARHGRLL 60

Query: 61  QSS-GGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSCNSCNGCPE 120
           QS  GGV++FPV G  DPFLVGLYYTKV+LG PP++F VQIDTGSDVLWVSC SCNGCP+
Sbjct: 61  QSPVGGVVNFPVDGASDPFLVGLYYTKVKLGTPPREFNVQIDTGSDVLWVSCTSCNGCPK 120

Query: 121 TSGLQIQLNFFDPASSSTASPVSCSDQICALGVQSSDSACLGQSNQCAYVFQYGDGSGTS 180
           TS LQIQL+FFDP  SS+AS VSCSD+ C    Q ++S C   +N C+Y F+YGDGSGTS
Sbjct: 121 TSELQIQLSFFDPGVSSSASLVSCSDRRCYSNFQ-TESGC-SPNNLCSYSFKYGDGSGTS 180

Query: 181 GFYVRDMMHLDIVVESSMTTNSSASVVFGCSTSQTGDLTKSDRAVDGIFGFGQHDLSVIS 240
           G+Y+ D M  D V+ S++  NSSA  VFGCS  Q+GDL +  RAVDGIFG GQ  LSVIS
Sbjct: 181 GYYISDFMSFDTVITSTLAINSSAPFVFGCSNLQSGDLQRPRRAVDGIFGLGQGSLSVIS 240

Query: 241 QLSSRGIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNLQSISVNGQ 300
           QL+ +G+AP+VFSHCLKGD SGGGI+VLG+I  P+ VYTPLVPSQPHYN+NLQSI+VNGQ
Sbjct: 241 QLAVQGLAPRVFSHCLKGDKSGGGIMVLGQIKRPDTVYTPLVPSQPHYNVNLQSIAVNGQ 300

Query: 301 MLPINPTVFTTTNGQGTIVDSGTTLAYLAEEAFNSFVVAITNLVSQTTQPVLLKGNQCYI 360
           +LPI+P+VFT   G GTI+D+GTTLAYL +EA++ F+ A+ N VSQ  +P+  +  QC+ 
Sbjct: 301 ILPIDPSVFTIATGDGTIIDTGTTLAYLPDEAYSPFIQAVANAVSQYGRPITYESYQCFE 360

Query: 361 TSSSISDTFPQVSLNFAGGASLVLRPQDYLIQQSSAGGNTVWCVGFQKIPGQGITILGDL 420
            ++   D FPQVSL+FAGGAS+VL P+ YL Q  S+ G+++WC+GFQ++  + ITILGDL
Sbjct: 361 ITAGDVDVFPQVSLSFAGGASMVLGPRAYL-QIFSSSGSSIWCIGFQRMSHRRITILGDL 420

Query: 421 VLKDKIFIYDLANQRIGWANYDCSMSVNVSTASKTGQSESV-NAGQLSGSGSVENQPDGL 480
           VLKDK+ +YDL  QRIGWA YDCS+ VNVS AS+ G+S+ V N GQ   SGS        
Sbjct: 421 VLKDKVVVYDLVRQRIGWAEYDCSLEVNVS-ASRGGRSKDVINTGQWRESGSESFNRSYY 480

Query: 481 ILQLSILVL 488
           +LQL + ++
Sbjct: 481 LLQLVVFLV 485

BLAST of MS005129 vs. TAIR 10
Match: AT2G36670.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 547.7 bits (1410), Expect = 9.4e-156
Identity = 290/506 (57.31%), Postives = 370/506 (73.12%), Query Frame = 0

Query: 1   MAAIVRTAVSV---AAAVLMLQAATVLCGFPAKLSLERAFPTNHGVEMAQLRGRDRARHG 60
           +AA +  A++V   AA+ L    A    G    L L+RAFP +  VE+++LR RDR RH 
Sbjct: 9   LAAALAVALAVTGFAASPLPSAYAKYAAGPTKILPLQRAFPLDELVELSELRARDRVRHA 68

Query: 61  RML------QSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTGSDVLWVSC 120
           R+L       S GGV+DFPV G+ DP+LVGLY+TKV+LG+PP +F VQIDTGSD+LWV+C
Sbjct: 69  RILLGGGRQSSVGGVVDFPVQGSSDPYLVGLYFTKVKLGSPPTEFNVQIDTGSDILWVTC 128

Query: 121 NSCNGCPETSGLQIQLNFFDPASSSTASPVSCSDQICALGVQSSDSACLGQSNQCAYVFQ 180
           +SC+ CP +SGL I L+FFD   S TA  V+CSD IC+   Q++ + C  ++NQC Y F+
Sbjct: 129 SSCSNCPHSSGLGIDLHFFDAPGSLTAGSVTCSDPICSSVFQTTAAQC-SENNQCGYSFR 188

Query: 181 YGDGSGTSGFYVRDMMHLDIVVESSMTTNSSASVVFGCSTSQTGDLTKSDRAVDGIFGFG 240
           YGDGSGTSG+Y+ D  + D ++  S+  NSSA +VFGCST Q+GDLTKSD+AVDGIFGFG
Sbjct: 189 YGDGSGTSGYYMTDTFYFDAILGESLVANSSAPIVFGCSTYQSGDLTKSDKAVDGIFGFG 248

Query: 241 QHDLSVISQLSSRGIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPHYNLNL 300
           +  LSV+SQLSSRGI P VFSHCLKGD SGGG+ VLGEI+ P +VY+PLVPSQPHYNLNL
Sbjct: 249 KGKLSVVSQLSSRGITPPVFSHCLKGDGSGGGVFVLGEILVPGMVYSPLVPSQPHYNLNL 308

Query: 301 QSISVNGQMLPINPTVFTTTNGQGTIVDSGTTLAYLAEEAFNSFVVAITNLVSQTTQPVL 360
            SI VNGQMLP++  VF  +N +GTIVD+GTTL YL +EA++ F+ AI+N VSQ   P++
Sbjct: 309 LSIGVNGQMLPLDAAVFEASNTRGTIVDTGTTLTYLVKEAYDLFLNAISNSVSQLVTPII 368

Query: 361 LKGNQCYITSSSISDTFPQVSLNFAGGASLVLRPQDYLIQQSSAGGNTVWCVGFQKIPGQ 420
             G QCY+ S+SISD FP VSLNFAGGAS++LRPQDYL       G ++WC+GFQK P +
Sbjct: 369 SNGEQCYLVSTSISDMFPSVSLNFAGGASMMLRPQDYLFHYGIYDGASMWCIGFQKAPEE 428

Query: 421 GITILGDLVLKDKIFIYDLANQRIGWANYDCSMSVNVSTASKTGQSESVNAGQLSGSGSV 480
             TILGDLVLKDK+F+YDLA QRIGWA+YDCSMSVNVS    T   + VN+GQ   + S 
Sbjct: 429 Q-TILGDLVLKDKVFVYDLARQRIGWASYDCSMSVNVSI---TSGKDIVNSGQPCLNIST 488

Query: 481 ENQPDGLILQLSILVLF-VQLSIFTS 497
            +    ++++L   +LF + L IF S
Sbjct: 489 RD----ILIRLFFSILFGLLLCIFFS 505

BLAST of MS005129 vs. TAIR 10
Match: AT2G36670.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 541.6 bits (1394), Expect = 6.7e-154
Identity = 290/511 (56.75%), Postives = 370/511 (72.41%), Query Frame = 0

Query: 1   MAAIVRTAVSV---AAAVLMLQAATVLCGFPAKLSLERAFPTNHGVEMAQLRGRDRARHG 60
           +AA +  A++V   AA+ L    A    G    L L+RAFP +  VE+++LR RDR RH 
Sbjct: 9   LAAALAVALAVTGFAASPLPSAYAKYAAGPTKILPLQRAFPLDELVELSELRARDRVRHA 68

Query: 61  RML------QSSGGVIDFPVAGTYDPFLVG-----LYYTKVQLGNPPKDFFVQIDTGSDV 120
           R+L       S GGV+DFPV G+ DP+LVG     LY+TKV+LG+PP +F VQIDTGSD+
Sbjct: 69  RILLGGGRQSSVGGVVDFPVQGSSDPYLVGSKMTMLYFTKVKLGSPPTEFNVQIDTGSDI 128

Query: 121 LWVSCNSCNGCPETSGLQIQLNFFDPASSSTASPVSCSDQICALGVQSSDSACLGQSNQC 180
           LWV+C+SC+ CP +SGL I L+FFD   S TA  V+CSD IC+   Q++ + C  ++NQC
Sbjct: 129 LWVTCSSCSNCPHSSGLGIDLHFFDAPGSLTAGSVTCSDPICSSVFQTTAAQC-SENNQC 188

Query: 181 AYVFQYGDGSGTSGFYVRDMMHLDIVVESSMTTNSSASVVFGCSTSQTGDLTKSDRAVDG 240
            Y F+YGDGSGTSG+Y+ D  + D ++  S+  NSSA +VFGCST Q+GDLTKSD+AVDG
Sbjct: 189 GYSFRYGDGSGTSGYYMTDTFYFDAILGESLVANSSAPIVFGCSTYQSGDLTKSDKAVDG 248

Query: 241 IFGFGQHDLSVISQLSSRGIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPSQPH 300
           IFGFG+  LSV+SQLSSRGI P VFSHCLKGD SGGG+ VLGEI+ P +VY+PLVPSQPH
Sbjct: 249 IFGFGKGKLSVVSQLSSRGITPPVFSHCLKGDGSGGGVFVLGEILVPGMVYSPLVPSQPH 308

Query: 301 YNLNLQSISVNGQMLPINPTVFTTTNGQGTIVDSGTTLAYLAEEAFNSFVVAITNLVSQT 360
           YNLNL SI VNGQMLP++  VF  +N +GTIVD+GTTL YL +EA++ F+ AI+N VSQ 
Sbjct: 309 YNLNLLSIGVNGQMLPLDAAVFEASNTRGTIVDTGTTLTYLVKEAYDLFLNAISNSVSQL 368

Query: 361 TQPVLLKGNQCYITSSSISDTFPQVSLNFAGGASLVLRPQDYLIQQSSAGGNTVWCVGFQ 420
             P++  G QCY+ S+SISD FP VSLNFAGGAS++LRPQDYL       G ++WC+GFQ
Sbjct: 369 VTPIISNGEQCYLVSTSISDMFPSVSLNFAGGASMMLRPQDYLFHYGIYDGASMWCIGFQ 428

Query: 421 KIPGQGITILGDLVLKDKIFIYDLANQRIGWANYDCSMSVNVSTASKTGQSESVNAGQLS 480
           K P +  TILGDLVLKDK+F+YDLA QRIGWA+YDCSMSVNVS    T   + VN+GQ  
Sbjct: 429 KAPEEQ-TILGDLVLKDKVFVYDLARQRIGWASYDCSMSVNVSI---TSGKDIVNSGQPC 488

Query: 481 GSGSVENQPDGLILQLSILVLF-VQLSIFTS 497
            + S  +    ++++L   +LF + L IF S
Sbjct: 489 LNISTRD----ILIRLFFSILFGLLLCIFFS 510

BLAST of MS005129 vs. TAIR 10
Match: AT3G02740.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 311.6 bits (797), Expect = 1.1e-84
Identity = 175/460 (38.04%), Postives = 261/460 (56.74%), Query Frame = 0

Query: 44  EMAQLRGRDRARHGRMLQSSGGVIDFPVAGTYDPFLVGLYYTKVQLGNPPKDFFVQIDTG 103
           ++  LR  D  RH R+L +    ID P+ G   P  +GLY+ K+ LG P +DF VQ+DTG
Sbjct: 50  DLGALRAHDVHRHSRLLSA----IDIPLGGDSQPESIGLYFAKIGLGTPSRDFHVQVDTG 109

Query: 104 SDVLWVSCNSCNGCPETSGLQIQLNFFDPASSSTASPVSCSDQICALGVQSSDSACLGQS 163
           SD+LWV+C  C  CP  S L ++L  +D  +SSTA  VSCSD  C+   Q S+       
Sbjct: 110 SDILWVNCAGCIRCPRKSDL-VELTPYDVDASSTAKSVSCSDNFCSYVNQRSE---CHSG 169

Query: 164 NQCAYVFQYGDGSGTSGFYVRDMMHLDIVVESSMTTNSSASVVFGCSTSQTGDLTKSDRA 223
           + C YV  YGDGS T+G+ V+D++HLD+V  +  T +++ +++FGC + Q+G L +S  A
Sbjct: 170 STCQYVIMYGDGSSTNGYLVKDVVHLDLVTGNRQTGSTNGTIIFGCGSKQSGQLGESQAA 229

Query: 224 VDGIFGFGQHDLSVISQLSSRGIAPKVFSHCLKGDDSGGGILVLGEIVEPNVVYTPLVPS 283
           VDGI GFGQ + S ISQL+S+G   + F+HCL  +++GGGI  +GE+V P V  TP++  
Sbjct: 230 VDGIMGFGQSNSSFISQLASQGKVKRSFAHCL-DNNNGGGIFAIGEVVSPKVKTTPMLSK 289

Query: 284 QPHYNLNLQSISVNGQMLPINPTVFTTTNGQGTIVDSGTTLAYLAEEAFNSFVVAITNLV 343
             HY++NL +I V   +L ++   F + + +G I+DSGTTL YL +  +N  +  I    
Sbjct: 290 SAHYSVNLNAIEVGNSVLELSSNAFDSGDDKGVIIDSGTTLVYLPDAVYNPLLNEILASH 349

Query: 344 SQTTQPVLLKGNQCYITSSSISDTFPQVSLNFAGGASLVLRPQDYLIQQSSAGGNTVWCV 403
            + T   + +   C+  +  + D FP V+  F    SL + P++YL Q         WC 
Sbjct: 350 PELTLHTVQESFTCFHYTDKL-DRFPTVTFQFDKSVSLAVYPREYLFQVR----EDTWCF 409

Query: 404 GFQK-----IPGQGITILGDLVLKDKIFIYDLANQRIGWANYDCSMSVNVSTASKTGQSE 463
           G+Q        G  +TILGD+ L +K+ +YD+ NQ IGW N++CS  + V    ++G   
Sbjct: 410 GWQNGGLQTKGGASLTILGDMALSNKLVVYDIENQVIGWTNHNCSGGIQVKD-EESGAIY 469

Query: 464 SVNAGQLSGSGSVENQPDGLILQLSILVLFVQLSIFTSFF 499
           +V A  LS S S           L+I  L   +S+   FF
Sbjct: 470 TVGAHNLSWSSS-----------LAITKLLTLVSLLIPFF 483

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140699.12.6e-28099.60aspartic proteinase-like protein 2 [Momordica charantia][more]
XP_038902575.11.7e-25288.20aspartic proteinase 39 [Benincasa hispida][more]
XP_022995311.11.4e-24987.98aspartic proteinase-like protein 2 [Cucurbita maxima][more]
KAG6606020.13.1e-24987.78Aspartic proteinase-like protein 2, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022958429.18.9e-24987.58aspartic proteinase-like protein 2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q4V3D22.1e-8339.20Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1[more]
Q9S9K42.9e-7739.95Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2[more]
Q8VYV91.5e-3330.29Aspartyl protease family protein 1 OS=Arabidopsis thaliana OX=3702 GN=APF1 PE=2 ... [more]
Q9LX202.9e-3228.17Aspartic proteinase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g10080 ... [more]
Q766C29.2e-3129.98Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A6J1CGE51.2e-28099.60aspartic proteinase-like protein 2 OS=Momordica charantia OX=3673 GN=LOC11101129... [more]
A0A6J1K1L86.6e-25087.98aspartic proteinase-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111490894 P... [more]
A0A6J1H5284.3e-24987.58aspartic proteinase-like protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111459652... [more]
A0A5A7SVM36.2e-24886.20Aspartic proteinase-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3C8H16.2e-24886.20aspartic proteinase-like protein 2 OS=Cucumis melo OX=3656 GN=LOC103497990 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT5G22850.12.4e-19968.98Eukaryotic aspartyl protease family protein [more]
AT1G08210.17.0e-15959.51Eukaryotic aspartyl protease family protein [more]
AT2G36670.29.4e-15657.31Eukaryotic aspartyl protease family protein [more]
AT2G36670.16.7e-15456.75Eukaryotic aspartyl protease family protein [more]
AT3G02740.11.1e-8438.04Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 89..109
score: 50.53
coord: 316..327
score: 45.59
coord: 414..429
score: 34.51
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 19..459
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 83..268
e-value: 1.5E-42
score: 145.9
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 270..449
e-value: 1.1E-46
score: 160.9
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 62..269
e-value: 5.7E-49
score: 168.7
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 78..446
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 286..438
e-value: 5.8E-26
score: 91.2
NoneNo IPR availablePANTHERPTHR13683:SF784EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEINcoord: 19..459
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 83..438
score: 47.534664
IPR034161Pepsin-like domain, plantCDDcd05476pepsin_A_like_plantcoord: 83..442
e-value: 5.36961E-70
score: 222.523

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS005129.1MS005129.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity