MS005091 (gene) Bitter gourd (TR) v1

Overview
NameMS005091
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionruBisCO large subunit-binding protein subunit alpha
Locationscaffold176: 2635526 .. 2638716 (+)
RNA-Seq ExpressionMS005091
SyntenyMS005091
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTCTGCTAATGCTATTTCTTCCGCATCCATTCTCTGTTCTTCTCAGAAGGTATTACTTCGCCTGCTTTCTCATTTCTTCGTTGTTTGGACTCTGTATTCTTGCTGATTCCTAGCTTTCCTTCGTCTTTGGAAATTATTGACATGTGGAGAATAGAAATTTTTGCACCGTCTGAGAACCATTTGAGCTACGTAATTGGAGTGTTGGGTAGAAGTTTTTGTGGAGTTAGCCATGAATATGATAGATGAGCTGAGATTGAGTGTTTGCTTCTTTTAAATGTGGGGGAGGAGGAAGTGATAATATTAATGTAGCATTTCCAGTAGCTTACTCTGCATTTGTCTGACGTTGCAGAACTTGAGGAAGGTGAATCAATCGCAGAGCAGCAGGCTAAATTACAGACAGGCGGGTAGCAGATTTGTTGTGAGAGCTACTGCAAAGGAGATAGCGTTCGACCAGAGTTCTAGGGCCGCACTTCAGTCCGGGATTGATAAGCTTGCCAATGCAGTCGGTCTGACTCTTGGACCTAGGGGTAACTTCCTGCTTCTTTTCGTTTATGAAGCACTTAGTATTGAGTTTCGTTTGTGAACTTTTCGTTGTCCTATCTTGCTTTTTGTTTTGTTTAGTTGTAGGAGTCTCTACAAGATATGTTCAATTCATTTGGAAATTGATATCTTTTTTCTGTTTATGGAAATGGATAATTCAACTTGTGCCCTTTATATTTGAAGGGAGGAACGTTGTGCTGGATGAGTTTGGGAGTCCCAAAGTAGTAAATGATGGTGTAACAATTGCTCGGGCCATTGAGTTGCCCGATGCTATGGAAAATGCTGGTGCAGCTCTGATTAGGGAGGTTGGCATTTTATACTTTAACTTGAACTTCCTCTTTTATACTGTGCGATCTATATTTTATCTGTTATGATGCTAAAACTTCTCATCTATCAGGCCCTGAGGTCCTGTAAGATTTTTCATTGCGTTGACAAGCATCATTTTTGTCATGAAGGTTGCTAGTAAGACTAATGATTCTGCTGGTGATGGGACAACAACTGCCTCCGTCCTCGCTAGGGAAATTATCAAACTAGGACTTCTCAGTGTTACCTCCGGAGCAAATCCAGTATCACTGAAGAAAGGAATTGACAAGACTGTGCAAGGATTGATAGAGGAGCTTGAGAATAAGGCTAGGGCCGTGAAAGGTGGAGATGATATCAAAGGTAGTGATTCCAATTTTCCGTATCCTGTATAGACCTCAATGTGGAACCTTTTGATTTCTTACCAAAATTTCTTCAATAATTGATCTTTGTTTTTATTTACTTCCGTGTAGCTGTTGCTTCTATTTCTGCTGGAAATGATGAGCTTATAGGGTCGATGATTGCTGATGCAATTGATAAAGTGGGGCCCGATGGTGTCTTATCCATTGAGTCATCATCTTCCTTTGAGACCACTGTTGAAGTTGAAGAAGGGATGGCGGTGTGTACTTTTTGCTCTTTTCTAAATTATCTTTCTCTGTCCTTCTGCATCATACATGCACATTCTCCACATTCTAACTTTATTCTTTACGTCTGTATTATTTTTTTTTGTTTTGCAGATTGACAGAGGCTATATCTCTCCTCAGTTTGTCACGAACCCTGAAAAGTTAATTGCTGAATTTGAGAATGCTAGAGTGTTAGTTACGGACCAGAAAATTACGGCTATAAAGGATATAATCCCTCTTTTGGAGAAAACGACACAATTGAGAGCTCCTTTGCTTATCATTGCAGAGGACGTTACTGGTGAGGCTTTGGCTACACTTGTTGTCAACAAGTTGCGGGGTATTCTAAATGTTGCTGCCATTAAAGCTCCGGGTTTTGGTGAAAGGAGAAAGGCTATGCTTCAAGACATTGCCATAATGACAGGTAGTCTAAGTTATCCTGGGCAATTACATGCTTCAGCTGTTTAAATGCTAATGAGTTTGCCCTTTGAGATTTTTCTTTTGCTTTACTTGTTTAGTTGATTGCAAAGTTGATTTTGTTTTCATGGATCCATGACTTCTCTATGCCATCCGTGTGAAATTTTATTATTTGGTTCAAATGAGTTTTATAGGGGGGAGGTCACATACTTTCGAACCCATCCGCTTTTATGTAGATGTGAACAGAATGGCTAACTTTTTACTTGTTACTTTATTATTTCAGGAGCTGAATTTCAAGCCAATGATCTTGGATTGCTTATTGAAAATACTTCAATTGAACAGCTTGGTTTGGCCCGAAAAGTGACCATCTCTAAGGACACAACCACCATTATTGCTGATGCTGCTTCAAAAGACGAGATTCAAGCTAGGATTGCGCAGATAAAGAAGGAATTGGCTGAGACAGATTCTGTTTACGACACAGAGAAACTGGCTGAAAGAATTGCCAAATTATCTGGTGGTGTTGCTGTCATTAAGGTAGGAGCTGCTACAGAGACTGAACTCGAGGACCGGAAGCTCCGTATTGAGGATGCAAAGAATGCAACTTTCGCCGCCATAGAGGAAGGGATTGTGCCTGGTGGTGGTGCTGCCCTGGTTCATCTCTCAACTTATGTCCCTGCAATTAAGGACAAGCTTGAAGATGCAGAGGAGAAGCTAGGTGCCGATATTATCCAAAAGGTAACTAACTCCTGTGCACGTAATATTGGACTTTGAATTTTCTCGTTATGCCTAAAAGAAGCAATCCAACCAAAAACGAGGAGAATGAAAGATTGGTATGTGTTGAGATTAGATATTTCTTTGATTTCAAAGTGGCATGACTGGTTATCACACGGTTTTAAGACATTTCTGGAGTTGCAACGTGTTGCAGGACCTCTCAAAAAGAGTCTACATGATGTAGTAAAGTGGTTAAGCTTGCGGTTCTGGTAGTATGTGAGTATTTTGTTGTTAATTTCGATCTTTTTGTATGTAGGCGCTGGTAGCACCAGCATCATTGATTGCCCAAAATGCTGGAGTTGAAGGGGAAGTAGTTGTGGAGAAGATCAAGGCGAGTGAATGGGAAATTGGGTACAATGCAATGACAGACAAGTACGAGAATCTTGTAGAGGCCGGTGTTATCGACCCGGCCAAGGTCACGAGATGTGCCCTACAGAACGCAGCTTCAGTTGCTGGGATGGTCCTAACCACACAGGCCATTGTAGTGGAAAAGCCCAAGCCCAAGGCACCCGTCGCTGCCCCACAAGGCCTTACCATT

mRNA sequence

ATGGCGTCTGCTAATGCTATTTCTTCCGCATCCATTCTCTGTTCTTCTCAGAAGAACTTGAGGAAGGTGAATCAATCGCAGAGCAGCAGGCTAAATTACAGACAGGCGGGTAGCAGATTTGTTGTGAGAGCTACTGCAAAGGAGATAGCGTTCGACCAGAGTTCTAGGGCCGCACTTCAGTCCGGGATTGATAAGCTTGCCAATGCAGTCGGTCTGACTCTTGGACCTAGGGGGAGGAACGTTGTGCTGGATGAGTTTGGGAGTCCCAAAGTAGTAAATGATGGTGTAACAATTGCTCGGGCCATTGAGTTGCCCGATGCTATGGAAAATGCTGGTGCAGCTCTGATTAGGGAGGTTGCTAGTAAGACTAATGATTCTGCTGGTGATGGGACAACAACTGCCTCCGTCCTCGCTAGGGAAATTATCAAACTAGGACTTCTCAGTGTTACCTCCGGAGCAAATCCAGTATCACTGAAGAAAGGAATTGACAAGACTGTGCAAGGATTGATAGAGGAGCTTGAGAATAAGGCTAGGGCCGTGAAAGGTGGAGATGATATCAAAGCTGTTGCTTCTATTTCTGCTGGAAATGATGAGCTTATAGGGTCGATGATTGCTGATGCAATTGATAAAGTGGGGCCCGATGGTGTCTTATCCATTGAGTCATCATCTTCCTTTGAGACCACTGTTGAAGTTGAAGAAGGGATGGCGATTGACAGAGGCTATATCTCTCCTCAGTTTGTCACGAACCCTGAAAAGTTAATTGCTGAATTTGAGAATGCTAGAGTGTTAGTTACGGACCAGAAAATTACGGCTATAAAGGATATAATCCCTCTTTTGGAGAAAACGACACAATTGAGAGCTCCTTTGCTTATCATTGCAGAGGACGTTACTGGTGAGGCTTTGGCTACACTTGTTGTCAACAAGTTGCGGGGTATTCTAAATGTTGCTGCCATTAAAGCTCCGGGTTTTGGTGAAAGGAGAAAGGCTATGCTTCAAGACATTGCCATAATGACAGGTACTGAATTTCAAGCCAATGATCTTGGATTGCTTATTGAAAATACTTCAATTGAACAGCTTGGTTTGGCCCGAAAAGTGACCATCTCTAAGGACACAACCACCATTATTGCTGATGCTGCTTCAAAAGACGAGATTCAAGCTAGGATTGCGCAGATAAAGAAGGAATTGGCTGAGACAGATTCTGTTTACGACACAGAGAAACTGGCTGAAAGAATTGCCAAATTATCTGGTGGTGTTGCTGTCATTAAGGTAGGAGCTGCTACAGAGACTGAACTCGAGGACCGGAAGCTCCGTATTGAGGATGCAAAGAATGCAACTTTCGCCGCCATAGAGGAAGGGATTGTGCCTGGTGGTGGTGCTGCCCTGGTTCATCTCTCAACTTATGTCCCTGCAATTAAGGACAAGCTTGAAGATGCAGAGGAGAAGCTAGGTGCCGATATTATCCAAAAGGCGCTGGTAGCACCAGCATCATTGATTGCCCAAAATGCTGGAGTTGAAGGGGAAGTAGTTGTGGAGAAGATCAAGGCGAGTGAATGGGAAATTGGGTACAATGCAATGACAGACAAGTACGAGAATCTTGTAGAGGCCGGTGTTATCGACCCGGCCAAGGTCACGAGATGTGCCCTACAGAACGCAGCTTCAGTTGCTGGGATGGTCCTAACCACACAGGCCATTGTAGTGGAAAAGCCCAAGCCCAAGGCACCCGTCGCTGCCCCACAAGGCCTTACCATT

Coding sequence (CDS)

ATGGCGTCTGCTAATGCTATTTCTTCCGCATCCATTCTCTGTTCTTCTCAGAAGAACTTGAGGAAGGTGAATCAATCGCAGAGCAGCAGGCTAAATTACAGACAGGCGGGTAGCAGATTTGTTGTGAGAGCTACTGCAAAGGAGATAGCGTTCGACCAGAGTTCTAGGGCCGCACTTCAGTCCGGGATTGATAAGCTTGCCAATGCAGTCGGTCTGACTCTTGGACCTAGGGGGAGGAACGTTGTGCTGGATGAGTTTGGGAGTCCCAAAGTAGTAAATGATGGTGTAACAATTGCTCGGGCCATTGAGTTGCCCGATGCTATGGAAAATGCTGGTGCAGCTCTGATTAGGGAGGTTGCTAGTAAGACTAATGATTCTGCTGGTGATGGGACAACAACTGCCTCCGTCCTCGCTAGGGAAATTATCAAACTAGGACTTCTCAGTGTTACCTCCGGAGCAAATCCAGTATCACTGAAGAAAGGAATTGACAAGACTGTGCAAGGATTGATAGAGGAGCTTGAGAATAAGGCTAGGGCCGTGAAAGGTGGAGATGATATCAAAGCTGTTGCTTCTATTTCTGCTGGAAATGATGAGCTTATAGGGTCGATGATTGCTGATGCAATTGATAAAGTGGGGCCCGATGGTGTCTTATCCATTGAGTCATCATCTTCCTTTGAGACCACTGTTGAAGTTGAAGAAGGGATGGCGATTGACAGAGGCTATATCTCTCCTCAGTTTGTCACGAACCCTGAAAAGTTAATTGCTGAATTTGAGAATGCTAGAGTGTTAGTTACGGACCAGAAAATTACGGCTATAAAGGATATAATCCCTCTTTTGGAGAAAACGACACAATTGAGAGCTCCTTTGCTTATCATTGCAGAGGACGTTACTGGTGAGGCTTTGGCTACACTTGTTGTCAACAAGTTGCGGGGTATTCTAAATGTTGCTGCCATTAAAGCTCCGGGTTTTGGTGAAAGGAGAAAGGCTATGCTTCAAGACATTGCCATAATGACAGGTACTGAATTTCAAGCCAATGATCTTGGATTGCTTATTGAAAATACTTCAATTGAACAGCTTGGTTTGGCCCGAAAAGTGACCATCTCTAAGGACACAACCACCATTATTGCTGATGCTGCTTCAAAAGACGAGATTCAAGCTAGGATTGCGCAGATAAAGAAGGAATTGGCTGAGACAGATTCTGTTTACGACACAGAGAAACTGGCTGAAAGAATTGCCAAATTATCTGGTGGTGTTGCTGTCATTAAGGTAGGAGCTGCTACAGAGACTGAACTCGAGGACCGGAAGCTCCGTATTGAGGATGCAAAGAATGCAACTTTCGCCGCCATAGAGGAAGGGATTGTGCCTGGTGGTGGTGCTGCCCTGGTTCATCTCTCAACTTATGTCCCTGCAATTAAGGACAAGCTTGAAGATGCAGAGGAGAAGCTAGGTGCCGATATTATCCAAAAGGCGCTGGTAGCACCAGCATCATTGATTGCCCAAAATGCTGGAGTTGAAGGGGAAGTAGTTGTGGAGAAGATCAAGGCGAGTGAATGGGAAATTGGGTACAATGCAATGACAGACAAGTACGAGAATCTTGTAGAGGCCGGTGTTATCGACCCGGCCAAGGTCACGAGATGTGCCCTACAGAACGCAGCTTCAGTTGCTGGGATGGTCCTAACCACACAGGCCATTGTAGTGGAAAAGCCCAAGCCCAAGGCACCCGTCGCTGCCCCACAAGGCCTTACCATT

Protein sequence

MASANAISSASILCSSQKNLRKVNQSQSSRLNYRQAGSRFVVRATAKEIAFDQSSRAALQSGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDAMENAGAALIREVASKTNDSAGDGTTTASVLAREIIKLGLLSVTSGANPVSLKKGIDKTVQGLIEELENKARAVKGGDDIKAVASISAGNDELIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMAIDRGYISPQFVTNPEKLIAEFENARVLVTDQKITAIKDIIPLLEKTTQLRAPLLIIAEDVTGEALATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAIMTGTEFQANDLGLLIENTSIEQLGLARKVTISKDTTTIIADAASKDEIQARIAQIKKELAETDSVYDTEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTYVPAIKDKLEDAEEKLGADIIQKALVAPASLIAQNAGVEGEVVVEKIKASEWEIGYNAMTDKYENLVEAGVIDPAKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPVAAPQGLTI
Homology
BLAST of MS005091 vs. NCBI nr
Match: XP_022140442.1 (ruBisCO large subunit-binding protein subunit alpha [Momordica charantia])

HSP 1 Score: 1062.4 bits (2746), Expect = 1.4e-306
Identity = 582/583 (99.83%), Postives = 582/583 (99.83%), Query Frame = 0

Query: 1   MASANAISSASILCSSQKNLRKVNQSQSSRLNYRQAGSRFVVRATAKEIAFDQSSRAALQ 60
           MASANAISSASILCSSQKNLRKVNQSQSSRLNYRQAGSRFVVRATAKEIAFDQSSRAALQ
Sbjct: 1   MASANAISSASILCSSQKNLRKVNQSQSSRLNYRQAGSRFVVRATAKEIAFDQSSRAALQ 60

Query: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDAMENAGAALIREVA 120
           SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDAMENAGAALIREVA
Sbjct: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDAMENAGAALIREVA 120

Query: 121 SKTNDSAGDGTTTASVLAREIIKLGLLSVTSGANPVSLKKGIDKTVQGLIEELENKARAV 180
           SKTNDSAGDGTTTASVLAREIIKLGLLSVTSGANPVSLKKGIDKTVQGLIEELENKARAV
Sbjct: 121 SKTNDSAGDGTTTASVLAREIIKLGLLSVTSGANPVSLKKGIDKTVQGLIEELENKARAV 180

Query: 181 KGGDDIKAVASISAGNDELIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240
           KGGDDIKAVASISAGNDELIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMAIDRG
Sbjct: 181 KGGDDIKAVASISAGNDELIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240

Query: 241 YISPQFVTNPEKLIAEFENARVLVTDQKITAIKDIIPLLEKTTQLRAPLLIIAEDVTGEA 300
           YISPQFVTNPEKLIAEFENARVLVTDQKITAIKDIIPLLEKTTQLRAPLLIIAEDVTGEA
Sbjct: 241 YISPQFVTNPEKLIAEFENARVLVTDQKITAIKDIIPLLEKTTQLRAPLLIIAEDVTGEA 300

Query: 301 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAIMTGTEFQANDLGLLIENTSIEQLG 360
           LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAIMTG EFQANDLGLLIENTSIEQLG
Sbjct: 301 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAIMTGAEFQANDLGLLIENTSIEQLG 360

Query: 361 LARKVTISKDTTTIIADAASKDEIQARIAQIKKELAETDSVYDTEKLAERIAKLSGGVAV 420
           LARKVTISKDTTTIIADAASKDEIQARIAQIKKELAETDSVYDTEKLAERIAKLSGGVAV
Sbjct: 361 LARKVTISKDTTTIIADAASKDEIQARIAQIKKELAETDSVYDTEKLAERIAKLSGGVAV 420

Query: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTYVPAIKDKLEDAEE 480
           IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTYVPAIKDKLEDAEE
Sbjct: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTYVPAIKDKLEDAEE 480

Query: 481 KLGADIIQKALVAPASLIAQNAGVEGEVVVEKIKASEWEIGYNAMTDKYENLVEAGVIDP 540
           KLGADIIQKALVAPASLIAQNAGVEGEVVVEKIKASEWEIGYNAMTDKYENLVEAGVIDP
Sbjct: 481 KLGADIIQKALVAPASLIAQNAGVEGEVVVEKIKASEWEIGYNAMTDKYENLVEAGVIDP 540

Query: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPVAAPQGLTI 584
           AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPVAAPQGLTI
Sbjct: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPVAAPQGLTI 583

BLAST of MS005091 vs. NCBI nr
Match: XP_022985565.1 (ruBisCO large subunit-binding protein subunit alpha-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1026.9 bits (2654), Expect = 6.6e-296
Identity = 555/583 (95.20%), Postives = 575/583 (98.63%), Query Frame = 0

Query: 1   MASANAISSASILCSSQKNLRKVNQSQSSRLNYRQAGSRFVVRATAKEIAFDQSSRAALQ 60
           MASANAISSASILCSSQK+LRK NQ+Q++RLNYRQAGSRFVVRATAKEIAFDQSSR+ALQ
Sbjct: 1   MASANAISSASILCSSQKSLRKANQTQNNRLNYRQAGSRFVVRATAKEIAFDQSSRSALQ 60

Query: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDAMENAGAALIREVA 120
           SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPD MENAGAALIREVA
Sbjct: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 120

Query: 121 SKTNDSAGDGTTTASVLAREIIKLGLLSVTSGANPVSLKKGIDKTVQGLIEELENKARAV 180
           SKTNDSAGDGTTTASVLAREIIKLGLL+VTSGANPVSLK+GIDKTVQGLI+ELENKARA+
Sbjct: 121 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI 180

Query: 181 KGGDDIKAVASISAGNDELIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240
           +G DDIKAVASISAGNDELIG MIADAI+KVGPDGVLSIESSSSFETTVEVEEGMAIDRG
Sbjct: 181 EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240

Query: 241 YISPQFVTNPEKLIAEFENARVLVTDQKITAIKDIIPLLEKTTQLRAPLLIIAEDVTGEA 300
           YISPQFVTNPEKLIAEFEN RVLVTDQKI+AIKDIIP+LEKTTQLRAPLLIIAEDVTGEA
Sbjct: 241 YISPQFVTNPEKLIAEFENTRVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA 300

Query: 301 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAIMTGTEFQANDLGLLIENTSIEQLG 360
           LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAI+TG EFQANDLGLL+ENTSIEQLG
Sbjct: 301 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTSIEQLG 360

Query: 361 LARKVTISKDTTTIIADAASKDEIQARIAQIKKELAETDSVYDTEKLAERIAKLSGGVAV 420
           LARKVTISKDTTTIIADAASKDE+QARIAQ+KKELAETDSVYDTEKLAERIAKLSGGVAV
Sbjct: 361 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV 420

Query: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTYVPAIKDKLEDAEE 480
           IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLST VPAIKDKLEDAEE
Sbjct: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE 480

Query: 481 KLGADIIQKALVAPASLIAQNAGVEGEVVVEKIKASEWEIGYNAMTDKYENLVEAGVIDP 540
           KLGADI+QKALVAPASLIAQNAG+EGEVVVEKIK+SEWEIGYNAMTDKYENLVEAGVIDP
Sbjct: 481 KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP 540

Query: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPVAAPQGLTI 584
           AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAP AAPQGLTI
Sbjct: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTI 583

BLAST of MS005091 vs. NCBI nr
Match: KAG6570801.1 (hypothetical protein SDJN03_29716, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1025.0 bits (2649), Expect = 2.5e-295
Identity = 554/583 (95.03%), Postives = 575/583 (98.63%), Query Frame = 0

Query: 1   MASANAISSASILCSSQKNLRKVNQSQSSRLNYRQAGSRFVVRATAKEIAFDQSSRAALQ 60
           MASANAISSASILCSSQK+LRK NQ+Q++RLNYRQAGSRFVVRATAKEIAFDQSSR+ALQ
Sbjct: 1   MASANAISSASILCSSQKSLRKANQTQNNRLNYRQAGSRFVVRATAKEIAFDQSSRSALQ 60

Query: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDAMENAGAALIREVA 120
           SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPD MENAGAALIREVA
Sbjct: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 120

Query: 121 SKTNDSAGDGTTTASVLAREIIKLGLLSVTSGANPVSLKKGIDKTVQGLIEELENKARAV 180
           SKTNDSAGDGTTTASVLAREIIKLGLL+VTSGANPVSLK+GIDKTVQGLI+ELENKARA+
Sbjct: 121 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI 180

Query: 181 KGGDDIKAVASISAGNDELIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240
           +G DDIKAVASISAGNDELIG MIADAI+KVGPDGVLSIESSSSFETTVEVEEGMAIDRG
Sbjct: 181 EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240

Query: 241 YISPQFVTNPEKLIAEFENARVLVTDQKITAIKDIIPLLEKTTQLRAPLLIIAEDVTGEA 300
           YISPQFVTNPEKLIAEFENARVLVTDQKI+AIKDIIP+LEKTTQLRAPLLIIAEDVTGEA
Sbjct: 241 YISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA 300

Query: 301 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAIMTGTEFQANDLGLLIENTSIEQLG 360
           LATLVVNKLRGILNVAAIKAP FGERRKAMLQDIAI+TG EFQANDLGLL+ENT+IEQLG
Sbjct: 301 LATLVVNKLRGILNVAAIKAPSFGERRKAMLQDIAILTGAEFQANDLGLLVENTTIEQLG 360

Query: 361 LARKVTISKDTTTIIADAASKDEIQARIAQIKKELAETDSVYDTEKLAERIAKLSGGVAV 420
           LARKVTISKDTTTIIADAASKDE+QARIAQ+KKELAETDSVYDTEKLAERIAKLSGGVAV
Sbjct: 361 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV 420

Query: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTYVPAIKDKLEDAEE 480
           IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLST VPAIKDKLEDAEE
Sbjct: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE 480

Query: 481 KLGADIIQKALVAPASLIAQNAGVEGEVVVEKIKASEWEIGYNAMTDKYENLVEAGVIDP 540
           KLGADI+QKALVAPASLIAQNAG+EGEVVVEKIK+SEWEIGYNAMTDKYENLVEAGVIDP
Sbjct: 481 KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP 540

Query: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPVAAPQGLTI 584
           AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAP AAPQGLTI
Sbjct: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTI 583

BLAST of MS005091 vs. NCBI nr
Match: QDE10505.1 (ruBisCO1 [Cucurbita pepo])

HSP 1 Score: 1025.0 bits (2649), Expect = 2.5e-295
Identity = 554/583 (95.03%), Postives = 575/583 (98.63%), Query Frame = 0

Query: 1   MASANAISSASILCSSQKNLRKVNQSQSSRLNYRQAGSRFVVRATAKEIAFDQSSRAALQ 60
           MASANAISSASILCSSQK+LRKVNQ QS+R+NYRQAGSRFVVRATAK+IAFDQSSR ALQ
Sbjct: 1   MASANAISSASILCSSQKSLRKVNQPQSNRVNYRQAGSRFVVRATAKDIAFDQSSRTALQ 60

Query: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDAMENAGAALIREVA 120
           SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPD MENAGAALIREVA
Sbjct: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 120

Query: 121 SKTNDSAGDGTTTASVLAREIIKLGLLSVTSGANPVSLKKGIDKTVQGLIEELENKARAV 180
           SKTNDSAGDGTTTASVLAREIIKLGLL+VTSGANPVSLK+GIDKTVQGLIEELE KARA+
Sbjct: 121 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIEELEKKARAI 180

Query: 181 KGGDDIKAVASISAGNDELIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240
           +G DDIKAVASISAGNDELIGSMIADAI+KVGPDGVLSIESSSSFETTVEVEEGMAIDRG
Sbjct: 181 EGRDDIKAVASISAGNDELIGSMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240

Query: 241 YISPQFVTNPEKLIAEFENARVLVTDQKITAIKDIIPLLEKTTQLRAPLLIIAEDVTGEA 300
           YISPQFVTNPEKLIAEFENARVLVTDQKI+AIKDIIP+LEKTTQLRAPLLIIAED+TGEA
Sbjct: 241 YISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDITGEA 300

Query: 301 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAIMTGTEFQANDLGLLIENTSIEQLG 360
           LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAI+TG EFQA+DLGLL+ENT+IEQLG
Sbjct: 301 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQASDLGLLVENTTIEQLG 360

Query: 361 LARKVTISKDTTTIIADAASKDEIQARIAQIKKELAETDSVYDTEKLAERIAKLSGGVAV 420
           LARKVTISKDTTTIIADAASKDE+QARIAQ+KKELAETDSVYDTEKLAERIAKLSGGVAV
Sbjct: 361 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV 420

Query: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTYVPAIKDKLEDAEE 480
           IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGG ALVHLST VPAIKDKLEDAEE
Sbjct: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGTALVHLSTLVPAIKDKLEDAEE 480

Query: 481 KLGADIIQKALVAPASLIAQNAGVEGEVVVEKIKASEWEIGYNAMTDKYENLVEAGVIDP 540
           KLGADIIQKALVAPASLIAQNAGVEGEVVVEKIK+SEWEIGYNAMTDKYENLVEAGVIDP
Sbjct: 481 KLGADIIQKALVAPASLIAQNAGVEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP 540

Query: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPVAAPQGLTI 584
           AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAP+AAPQGLT+
Sbjct: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPIAAPQGLTV 583

BLAST of MS005091 vs. NCBI nr
Match: XP_022943380.1 (ruBisCO large subunit-binding protein subunit alpha-like [Cucurbita moschata])

HSP 1 Score: 1024.6 bits (2648), Expect = 3.3e-295
Identity = 553/583 (94.85%), Postives = 575/583 (98.63%), Query Frame = 0

Query: 1   MASANAISSASILCSSQKNLRKVNQSQSSRLNYRQAGSRFVVRATAKEIAFDQSSRAALQ 60
           MASANAISSASILCSSQK+LRK NQ+Q++RLNYRQAGSRFVVRATAKEIAFDQSSR+ALQ
Sbjct: 1   MASANAISSASILCSSQKSLRKANQTQNNRLNYRQAGSRFVVRATAKEIAFDQSSRSALQ 60

Query: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDAMENAGAALIREVA 120
           SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPD MENAGAALIREVA
Sbjct: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 120

Query: 121 SKTNDSAGDGTTTASVLAREIIKLGLLSVTSGANPVSLKKGIDKTVQGLIEELENKARAV 180
           SKTNDSAGDGTTTASVLAREIIKLGLL+VTSGANPVSLK+GIDKTVQGLI+ELENKARA+
Sbjct: 121 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI 180

Query: 181 KGGDDIKAVASISAGNDELIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240
           +G DDIKAVASISAGNDELIG MIADAI+KVGPDGVLSIESSSSFETTVEVEEGMAIDRG
Sbjct: 181 EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240

Query: 241 YISPQFVTNPEKLIAEFENARVLVTDQKITAIKDIIPLLEKTTQLRAPLLIIAEDVTGEA 300
           YISPQFVTNPEKLIAEFENARVLVTDQKI+AIKDIIP+LEKTTQLRAPLLIIAEDVTGEA
Sbjct: 241 YISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA 300

Query: 301 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAIMTGTEFQANDLGLLIENTSIEQLG 360
           LATLVVNKLRGILNVAAIKAP FGERRKAMLQDIAI+TG EFQANDLGLL+ENT+IEQLG
Sbjct: 301 LATLVVNKLRGILNVAAIKAPSFGERRKAMLQDIAILTGAEFQANDLGLLVENTTIEQLG 360

Query: 361 LARKVTISKDTTTIIADAASKDEIQARIAQIKKELAETDSVYDTEKLAERIAKLSGGVAV 420
           LARKVTISKDTTTIIADAASKDE+QARIAQ+KKELAETDSVYDTEKLAERIAKLSGGVAV
Sbjct: 361 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV 420

Query: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTYVPAIKDKLEDAEE 480
           IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLST VPAIKDKLEDAEE
Sbjct: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE 480

Query: 481 KLGADIIQKALVAPASLIAQNAGVEGEVVVEKIKASEWEIGYNAMTDKYENLVEAGVIDP 540
           KLGADI+QKALVAPASLIAQNAG+EGEVVVEKIK+SEWEIGYNAMTDKYENLVEAGVIDP
Sbjct: 481 KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP 540

Query: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPVAAPQGLTI 584
           AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAP AAPQGLT+
Sbjct: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTV 583

BLAST of MS005091 vs. ExPASy Swiss-Prot
Match: P08926 (RuBisCO large subunit-binding protein subunit alpha, chloroplastic OS=Pisum sativum OX=3888 PE=1 SV=2)

HSP 1 Score: 934.1 bits (2413), Expect = 7.7e-271
Identity = 507/588 (86.22%), Postives = 551/588 (93.71%), Query Frame = 0

Query: 1   MASANAISSASILCS----SQKNLRKVNQSQSSRLNYRQAGSRFVVRATAKEIAFDQSSR 60
           MAS NA+SS SIL S    +Q +L K    Q  R+N+RQ  +RFVV+A AK+IAFDQ SR
Sbjct: 1   MASTNALSSTSILRSPTNQAQTSLSK-KVKQHGRVNFRQKPNRFVVKAAAKDIAFDQHSR 60

Query: 61  AALQSGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDAMENAGAALI 120
           +A+Q+GIDKLA+AVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPD MENAGAALI
Sbjct: 61  SAMQAGIDKLADAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALI 120

Query: 121 REVASKTNDSAGDGTTTASVLAREIIKLGLLSVTSGANPVSLKKGIDKTVQGLIEELENK 180
           REVASKTNDSAGDGTTTAS+LAREIIKLGLL+VTSGANPVS+KKGIDKTV  L+EELE  
Sbjct: 121 REVASKTNDSAGDGTTTASILAREIIKLGLLNVTSGANPVSIKKGIDKTVAALVEELEKL 180

Query: 181 ARAVKGGDDIKAVASISAGNDELIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMA 240
           AR VKGGDDIKAVA+ISAGNDELIG MIA+AIDKVGPDGVLSIESS+SFETTVEVEEGM 
Sbjct: 181 ARPVKGGDDIKAVATISAGNDELIGKMIAEAIDKVGPDGVLSIESSNSFETTVEVEEGME 240

Query: 241 IDRGYISPQFVTNPEKLIAEFENARVLVTDQKITAIKDIIPLLEKTTQLRAPLLIIAEDV 300
           IDRGYISPQFVTNPEK I EFENARVL+TDQKI+AIKDIIPLLEKTTQLRAPLLII+ED+
Sbjct: 241 IDRGYISPQFVTNPEKSIVEFENARVLITDQKISAIKDIIPLLEKTTQLRAPLLIISEDI 300

Query: 301 TGEALATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAIMTGTEFQANDLGLLIENTSI 360
           TGEALATLVVNKLRGILNVAAIKAPGFGERRKA+LQDIAI+TG EFQA+DLGLL+ENT+I
Sbjct: 301 TGEALATLVVNKLRGILNVAAIKAPGFGERRKALLQDIAILTGAEFQASDLGLLVENTTI 360

Query: 361 EQLGLARKVTISKDTTTIIADAASKDEIQARIAQIKKELAETDSVYDTEKLAERIAKLSG 420
           EQLGLARKVTISKD+TTIIADAASKDE+Q+R+AQ+KKEL+ETDS+YD+EKLAERIAKLSG
Sbjct: 361 EQLGLARKVTISKDSTTIIADAASKDELQSRVAQLKKELSETDSIYDSEKLAERIAKLSG 420

Query: 421 GVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTYVPAIKDKLE 480
           GVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGG ALVHLS YVPAIK+KLE
Sbjct: 421 GVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGTALVHLSGYVPAIKEKLE 480

Query: 481 DAEEKLGADIIQKALVAPASLIAQNAGVEGEVVVEKIKASEWEIGYNAMTDKYENLVEAG 540
           DA+E+LGADI+QKALVAPA+LIAQNAG+EGEVVVEKIK  EWE+GYNAMTD YENLVE+G
Sbjct: 481 DADERLGADIVQKALVAPAALIAQNAGIEGEVVVEKIKNGEWEVGYNAMTDTYENLVESG 540

Query: 541 VIDPAKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPV-AAPQGLTI 584
           VIDPAKVTRCALQNAASVAGMVLTTQAIVVEKPKPKA V AAPQGLTI
Sbjct: 541 VIDPAKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAAVAAAPQGLTI 587

BLAST of MS005091 vs. ExPASy Swiss-Prot
Match: P21238 (Chaperonin 60 subunit alpha 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CPN60A1 PE=1 SV=2)

HSP 1 Score: 923.3 bits (2385), Expect = 1.4e-267
Identity = 502/586 (85.67%), Postives = 545/586 (93.00%), Query Frame = 0

Query: 1   MASANAISSASILCSS-QKNLRKVNQSQSSRLNY-RQAGSRFVVRATAKEIAFDQSSRAA 60
           MASANA+SSAS+LCSS Q  L   NQ Q  R++Y ++   RF VRA  KEIAFDQ SRAA
Sbjct: 1   MASANALSSASVLCSSRQSKLGGGNQQQGQRVSYNKRTIRRFSVRANVKEIAFDQHSRAA 60

Query: 61  LQSGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDAMENAGAALIRE 120
           LQ+GIDKLA+ VGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELP+AMENAGAALIRE
Sbjct: 61  LQAGIDKLADCVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPNAMENAGAALIRE 120

Query: 121 VASKTNDSAGDGTTTASVLAREIIKLGLLSVTSGANPVSLKKGIDKTVQGLIEELENKAR 180
           VASKTNDSAGDGTTTAS+LAREIIK GLLSVTSGANPVSLK+GIDKTVQGLIEEL+ KAR
Sbjct: 121 VASKTNDSAGDGTTTASILAREIIKHGLLSVTSGANPVSLKRGIDKTVQGLIEELQKKAR 180

Query: 181 AVKGGDDIKAVASISAGNDELIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMAID 240
            VKG DDI+AVASISAGND+LIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGM ID
Sbjct: 181 PVKGRDDIRAVASISAGNDDLIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMEID 240

Query: 241 RGYISPQFVTNPEKLIAEFENARVLVTDQKITAIKDIIPLLEKTTQLRAPLLIIAEDVTG 300
           RGYISPQFVTNPEKL+AEFENARVL+TDQKITAIKDIIP+LEKTTQLRAPLLIIAEDVTG
Sbjct: 241 RGYISPQFVTNPEKLLAEFENARVLITDQKITAIKDIIPILEKTTQLRAPLLIIAEDVTG 300

Query: 301 EALATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAIMTGTEFQANDLGLLIENTSIEQ 360
           EALATLVVNKLRG+LNV A+KAPGFGERRKAMLQDIAI+TG E+ A D+ LL+EN +I+Q
Sbjct: 301 EALATLVVNKLRGVLNVVAVKAPGFGERRKAMLQDIAILTGAEYLAMDMSLLVENATIDQ 360

Query: 361 LGLARKVTISKDTTTIIADAASKDEIQARIAQIKKELAETDSVYDTEKLAERIAKLSGGV 420
           LG+ARKVTISKD+TT+IADAASKDE+QARIAQ+KKEL ETDSVYD+EKLAERIAKLSGGV
Sbjct: 361 LGIARKVTISKDSTTLIADAASKDELQARIAQLKKELFETDSVYDSEKLAERIAKLSGGV 420

Query: 421 AVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTYVPAIKDKLEDA 480
           AVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLST +PAIK+  EDA
Sbjct: 421 AVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTVIPAIKETFEDA 480

Query: 481 EEKLGADIIQKALVAPASLIAQNAGVEGEVVVEKIKASEWEIGYNAMTDKYENLVEAGVI 540
           +E+LGADI+QKAL++PA+LIAQNAGVEGEVVVEKI  S+WE GYNAMTD YENL EAGVI
Sbjct: 481 DERLGADIVQKALLSPAALIAQNAGVEGEVVVEKIMFSDWENGYNAMTDTYENLFEAGVI 540

Query: 541 DPAKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAP-VAAPQGLTI 584
           DPAKVTRCALQNAASVAGMVLTTQAIVV+KPKPKAP  AAP+GL +
Sbjct: 541 DPAKVTRCALQNAASVAGMVLTTQAIVVDKPKPKAPAAAAPEGLMV 586

BLAST of MS005091 vs. ExPASy Swiss-Prot
Match: P21239 (RuBisCO large subunit-binding protein subunit alpha, chloroplastic (Fragment) OS=Brassica napus OX=3708 PE=2 SV=2)

HSP 1 Score: 916.8 bits (2368), Expect = 1.3e-265
Identity = 487/546 (89.19%), Postives = 525/546 (96.15%), Query Frame = 0

Query: 39  RFVVRATAKEIAFDQSSRAALQSGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTI 98
           RF VRA  KEI+FDQSSRAALQ+GIDKLA+AVGLTLGPRGRNVVLDEFGSPKVVNDGVTI
Sbjct: 1   RFSVRANVKEISFDQSSRAALQAGIDKLADAVGLTLGPRGRNVVLDEFGSPKVVNDGVTI 60

Query: 99  ARAIELPDAMENAGAALIREVASKTNDSAGDGTTTASVLAREIIKLGLLSVTSGANPVSL 158
           ARAIELPDAMENAGAALIREVASKTNDSAGDGTTTASVLAREIIK GLLSVTSGANPVSL
Sbjct: 61  ARAIELPDAMENAGAALIREVASKTNDSAGDGTTTASVLAREIIKHGLLSVTSGANPVSL 120

Query: 159 KKGIDKTVQGLIEELENKARAVKGGDDIKAVASISAGNDELIGSMIADAIDKVGPDGVLS 218
           K+GIDKTVQ LIEELE +AR VKGG DIKAVA+ISAGNDEL+G+MIADAIDKVGPDGVLS
Sbjct: 121 KRGIDKTVQALIEELEKRARPVKGGSDIKAVATISAGNDELVGTMIADAIDKVGPDGVLS 180

Query: 219 IESSSSFETTVEVEEGMAIDRGYISPQFVTNPEKLIAEFENARVLVTDQKITAIKDIIPL 278
           IESSSSFETTVEVEEGM IDRGYISPQFVTNPEKL+ EFENARVL+TDQKITAIKDIIP+
Sbjct: 181 IESSSSFETTVEVEEGMEIDRGYISPQFVTNPEKLLVEFENARVLITDQKITAIKDIIPI 240

Query: 279 LEKTTQLRAPLLIIAEDVTGEALATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAIMT 338
           LEKTTQLRAPLLIIAEDVTGEALATLVVNKLRG+LNV A+KAPGFGERRKAMLQDIAI+T
Sbjct: 241 LEKTTQLRAPLLIIAEDVTGEALATLVVNKLRGVLNVVAVKAPGFGERRKAMLQDIAILT 300

Query: 339 GTEFQANDLGLLIENTSIEQLGLARKVTISKDTTTIIADAASKDEIQARIAQIKKELAET 398
           G E+QA D+GLL+ENT+I+QLG+ARKVTISKD+TT+IADAASKDE+QARI+Q+KKEL+ET
Sbjct: 301 GAEYQALDMGLLVENTTIDQLGIARKVTISKDSTTLIADAASKDELQARISQLKKELSET 360

Query: 399 DSVYDTEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGG 458
           DSVYD+EKLAERIAKL+GGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGG
Sbjct: 361 DSVYDSEKLAERIAKLAGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGG 420

Query: 459 AALVHLSTYVPAIKDKLEDAEEKLGADIIQKALVAPASLIAQNAGVEGEVVVEKIKASEW 518
           A LVHLST +PAIK+KLEDA+E+LGADI+QKALVAPA+LIAQNAG+EGEVVVEKI  SEW
Sbjct: 421 ATLVHLSTVIPAIKEKLEDADERLGADIVQKALVAPAALIAQNAGIEGEVVVEKIMFSEW 480

Query: 519 EIGYNAMTDKYENLVEAGVIDPAKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPVAA- 578
           EIGYNAMTD YENL+EAGVIDPAKVTRCALQNAASVAGMVLTTQAIVV+KPKPKAP AA 
Sbjct: 481 EIGYNAMTDTYENLLEAGVIDPAKVTRCALQNAASVAGMVLTTQAIVVDKPKPKAPTAAP 540

Query: 579 PQGLTI 584
           PQGL +
Sbjct: 541 PQGLMV 546

BLAST of MS005091 vs. ExPASy Swiss-Prot
Match: P34794 (RuBisCO large subunit-binding protein subunit alpha, chloroplastic OS=Brassica napus OX=3708 PE=2 SV=1)

HSP 1 Score: 900.2 bits (2325), Expect = 1.2e-260
Identity = 494/585 (84.44%), Postives = 538/585 (91.97%), Query Frame = 0

Query: 1   MASANAISSASILCSS-QKNLRKVNQSQSSRLNYRQAGSRFVVRATAKEIAFDQSSRAAL 60
           MA+ANA+SS S+LCSS Q  L   +Q +  R++YR+A  RF +RA  KEIAFDQSSRAAL
Sbjct: 1   MATANALSSPSVLCSSRQGKLSGGSQQKGQRVSYRKANRRFSLRANVKEIAFDQSSRAAL 60

Query: 61  QSGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDAMENAGAALIREV 120
           Q+GIDKLA+AVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDAMENAGAALIREV
Sbjct: 61  QAGIDKLADAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDAMENAGAALIREV 120

Query: 121 ASKTNDSAGDGTTTASVLAREIIKLGLLSVTSGANPVSLKKGIDKTVQGLIEELENKARA 180
           ASKTNDSAGDGTTTASVLAREIIK GLLSVTSGANPVSLK+GIDKTVQ LIEELE ++R 
Sbjct: 121 ASKTNDSAGDGTTTASVLAREIIKHGLLSVTSGANPVSLKRGIDKTVQALIEELEKRSRP 180

Query: 181 VKGGDDIKAVASISAGNDELIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMAIDR 240
           VKGG DIKAVA+ISAGNDELIG+MIADAIDKVGPDGV  IESSSSFETTVEVEEGM IDR
Sbjct: 181 VKGGRDIKAVATISAGNDELIGAMIADAIDKVGPDGVSPIESSSSFETTVEVEEGMEIDR 240

Query: 241 GYISPQFVTNPEKLIAEFENARVLVTDQKITAIKDIIPLLEKTTQLRAPLLIIAEDVTGE 300
           GYISPQFVTNPEKL+ EFENARVL+TDQKITAIKDIIP+LEKTTQLRAPLLIIAEDVTGE
Sbjct: 241 GYISPQFVTNPEKLLVEFENARVLITDQKITAIKDIIPILEKTTQLRAPLLIIAEDVTGE 300

Query: 301 ALATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAIMTGTEFQANDLGLLIENTSIEQL 360
           ALATLVVNKLRG+LNV A+KAPGFGERRKAMLQDIAI+T     A D+GLL+ENT+I+QL
Sbjct: 301 ALATLVVNKLRGVLNVVAVKAPGFGERRKAMLQDIAILTEPS-TALDMGLLVENTTIDQL 360

Query: 361 GLARKVTISKDTTTIIADAASKDEIQARIAQIKKELAETDSVYDTEKLAERIAKLSGGVA 420
           G+ARKVTISKD+TT+IADAASK E+QARI+Q+KKE  ETDSVYD+EKLAERIAKLSGGVA
Sbjct: 361 GIARKVTISKDSTTLIADAASKAELQARISQLKKESFETDSVYDSEKLAERIAKLSGGVA 420

Query: 421 VIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTYVPAIKDKLEDAE 480
           VIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGA LVHLST +PAIK+  EDA+
Sbjct: 421 VIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGATLVHLSTVIPAIKETFEDAD 480

Query: 481 EKLGADIIQKALVAPASLIAQNAGVEGEVVVEKIKASEWEIGYNAMTDKYENLVEAGVID 540
            +LGADI+QKALVA  SLIAQNAG+EGEVVVEKI  SEWE+GYNAMTD YENL+EAGVID
Sbjct: 481 VRLGADIVQKALVA-QSLIAQNAGIEGEVVVEKIMFSEWELGYNAMTDTYENLLEAGVID 540

Query: 541 PAKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAP-VAAPQGLTI 584
           PAKVTRCALQNAASVAGMVLTTQAIVV+KPKPKAP  AAP+GL +
Sbjct: 541 PAKVTRCALQNAASVAGMVLTTQAIVVDKPKPKAPAAAAPEGLMV 583

BLAST of MS005091 vs. ExPASy Swiss-Prot
Match: P08823 (RuBisCO large subunit-binding protein subunit alpha, chloroplastic (Fragment) OS=Triticum aestivum OX=4565 PE=1 SV=1)

HSP 1 Score: 867.8 bits (2241), Expect = 6.7e-251
Identity = 460/535 (85.98%), Postives = 502/535 (93.83%), Query Frame = 0

Query: 44  ATAKEIAFDQSSRAALQSGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIE 103
           A AKEIAFDQ SRAALQ+G++KLANAVG+TLGPRGRNVVLDE+G+PKVVNDGVTIARAIE
Sbjct: 2   ADAKEIAFDQKSRAALQAGVEKLANAVGVTLGPRGRNVVLDEYGNPKVVNDGVTIARAIE 61

Query: 104 LPDAMENAGAALIREVASKTNDSAGDGTTTASVLAREIIKLGLLSVTSGANPVSLKKGID 163
           L + MENAGAALIREVASKTNDSAGDGTTTA VLAREIIKLG+LSVTSGANPVSLKKGID
Sbjct: 62  LANPMENAGAALIREVASKTNDSAGDGTTTACVLAREIIKLGILSVTSGANPVSLKKGID 121

Query: 164 KTVQGLIEELENKARAVKGGDDIKAVASISAGNDELIGSMIADAIDKVGPDGVLSIESSS 223
           KTVQGLIEELE KAR VKG  DIKAVASISAGNDELIG+MIADAIDKVGPDGVLSIESSS
Sbjct: 122 KTVQGLIEELERKARPVKGSGDIKAVASISAGNDELIGAMIADAIDKVGPDGVLSIESSS 181

Query: 224 SFETTVEVEEGMAIDRGYISPQFVTNPEKLIAEFENARVLVTDQKITAIKDIIPLLEKTT 283
           SFETTV+VEEGM IDRGYISPQFVTN EK I EFENARVL+TDQKIT+IK+IIPLLE+TT
Sbjct: 182 SFETTVDVEEGMEIDRGYISPQFVTNLEKSIVEFENARVLITDQKITSIKEIIPLLEQTT 241

Query: 284 QLRAPLLIIAEDVTGEALATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAIMTGTEFQ 343
           QLR PL I+AED+TGEALATLVVNKLRGI+NVAAIKAP FGERRKA+LQDIAI+TG E+ 
Sbjct: 242 QLRCPLFIVAEDITGEALATLVVNKLRGIINVAAIKAPSFGERRKAVLQDIAIVTGAEYL 301

Query: 344 ANDLGLLIENTSIEQLGLARKVTISKDTTTIIADAASKDEIQARIAQIKKELAETDSVYD 403
           A DLGLL+EN +++QLG ARK+TI + TTT+IADAASKDEIQAR+AQ+KKEL+ETDS+YD
Sbjct: 302 AKDLGLLVENATVDQLGTARKITIHQTTTTLIADAASKDEIQARVAQLKKELSETDSIYD 361

Query: 404 TEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVH 463
           +EKLAERIAKLSGGVAVIKVGA TETELEDR+LRIEDAKNATFAAIEEGIVPGGGAA VH
Sbjct: 362 SEKLAERIAKLSGGVAVIKVGATTETELEDRQLRIEDAKNATFAAIEEGIVPGGGAAYVH 421

Query: 464 LSTYVPAIKDKLEDAEEKLGADIIQKALVAPASLIAQNAGVEGEVVVEKIKASEWEIGYN 523
           LSTYVPAIK+ +ED +E+LGADIIQKAL APASLIA NAGVEGEVV+EKIK SEWE+GYN
Sbjct: 422 LSTYVPAIKETIEDHDERLGADIIQKALQAPASLIANNAGVEGEVVIEKIKESEWEMGYN 481

Query: 524 AMTDKYENLVEAGVIDPAKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPVAAP 579
           AMTDKYENL+E+GVIDPAKVTRCALQNAASV+GMVLTTQAIVVEKPKPK  VA P
Sbjct: 482 AMTDKYENLIESGVIDPAKVTRCALQNAASVSGMVLTTQAIVVEKPKPKPKVAEP 536

BLAST of MS005091 vs. ExPASy TrEMBL
Match: A0A6J1CGX6 (ruBisCO large subunit-binding protein subunit alpha OS=Momordica charantia OX=3673 GN=LOC111011118 PE=3 SV=1)

HSP 1 Score: 1062.4 bits (2746), Expect = 6.9e-307
Identity = 582/583 (99.83%), Postives = 582/583 (99.83%), Query Frame = 0

Query: 1   MASANAISSASILCSSQKNLRKVNQSQSSRLNYRQAGSRFVVRATAKEIAFDQSSRAALQ 60
           MASANAISSASILCSSQKNLRKVNQSQSSRLNYRQAGSRFVVRATAKEIAFDQSSRAALQ
Sbjct: 1   MASANAISSASILCSSQKNLRKVNQSQSSRLNYRQAGSRFVVRATAKEIAFDQSSRAALQ 60

Query: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDAMENAGAALIREVA 120
           SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDAMENAGAALIREVA
Sbjct: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDAMENAGAALIREVA 120

Query: 121 SKTNDSAGDGTTTASVLAREIIKLGLLSVTSGANPVSLKKGIDKTVQGLIEELENKARAV 180
           SKTNDSAGDGTTTASVLAREIIKLGLLSVTSGANPVSLKKGIDKTVQGLIEELENKARAV
Sbjct: 121 SKTNDSAGDGTTTASVLAREIIKLGLLSVTSGANPVSLKKGIDKTVQGLIEELENKARAV 180

Query: 181 KGGDDIKAVASISAGNDELIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240
           KGGDDIKAVASISAGNDELIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMAIDRG
Sbjct: 181 KGGDDIKAVASISAGNDELIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240

Query: 241 YISPQFVTNPEKLIAEFENARVLVTDQKITAIKDIIPLLEKTTQLRAPLLIIAEDVTGEA 300
           YISPQFVTNPEKLIAEFENARVLVTDQKITAIKDIIPLLEKTTQLRAPLLIIAEDVTGEA
Sbjct: 241 YISPQFVTNPEKLIAEFENARVLVTDQKITAIKDIIPLLEKTTQLRAPLLIIAEDVTGEA 300

Query: 301 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAIMTGTEFQANDLGLLIENTSIEQLG 360
           LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAIMTG EFQANDLGLLIENTSIEQLG
Sbjct: 301 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAIMTGAEFQANDLGLLIENTSIEQLG 360

Query: 361 LARKVTISKDTTTIIADAASKDEIQARIAQIKKELAETDSVYDTEKLAERIAKLSGGVAV 420
           LARKVTISKDTTTIIADAASKDEIQARIAQIKKELAETDSVYDTEKLAERIAKLSGGVAV
Sbjct: 361 LARKVTISKDTTTIIADAASKDEIQARIAQIKKELAETDSVYDTEKLAERIAKLSGGVAV 420

Query: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTYVPAIKDKLEDAEE 480
           IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTYVPAIKDKLEDAEE
Sbjct: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTYVPAIKDKLEDAEE 480

Query: 481 KLGADIIQKALVAPASLIAQNAGVEGEVVVEKIKASEWEIGYNAMTDKYENLVEAGVIDP 540
           KLGADIIQKALVAPASLIAQNAGVEGEVVVEKIKASEWEIGYNAMTDKYENLVEAGVIDP
Sbjct: 481 KLGADIIQKALVAPASLIAQNAGVEGEVVVEKIKASEWEIGYNAMTDKYENLVEAGVIDP 540

Query: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPVAAPQGLTI 584
           AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPVAAPQGLTI
Sbjct: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPVAAPQGLTI 583

BLAST of MS005091 vs. ExPASy TrEMBL
Match: A0A6J1JBN6 (ruBisCO large subunit-binding protein subunit alpha-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483598 PE=3 SV=1)

HSP 1 Score: 1026.9 bits (2654), Expect = 3.2e-296
Identity = 555/583 (95.20%), Postives = 575/583 (98.63%), Query Frame = 0

Query: 1   MASANAISSASILCSSQKNLRKVNQSQSSRLNYRQAGSRFVVRATAKEIAFDQSSRAALQ 60
           MASANAISSASILCSSQK+LRK NQ+Q++RLNYRQAGSRFVVRATAKEIAFDQSSR+ALQ
Sbjct: 1   MASANAISSASILCSSQKSLRKANQTQNNRLNYRQAGSRFVVRATAKEIAFDQSSRSALQ 60

Query: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDAMENAGAALIREVA 120
           SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPD MENAGAALIREVA
Sbjct: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 120

Query: 121 SKTNDSAGDGTTTASVLAREIIKLGLLSVTSGANPVSLKKGIDKTVQGLIEELENKARAV 180
           SKTNDSAGDGTTTASVLAREIIKLGLL+VTSGANPVSLK+GIDKTVQGLI+ELENKARA+
Sbjct: 121 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI 180

Query: 181 KGGDDIKAVASISAGNDELIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240
           +G DDIKAVASISAGNDELIG MIADAI+KVGPDGVLSIESSSSFETTVEVEEGMAIDRG
Sbjct: 181 EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240

Query: 241 YISPQFVTNPEKLIAEFENARVLVTDQKITAIKDIIPLLEKTTQLRAPLLIIAEDVTGEA 300
           YISPQFVTNPEKLIAEFEN RVLVTDQKI+AIKDIIP+LEKTTQLRAPLLIIAEDVTGEA
Sbjct: 241 YISPQFVTNPEKLIAEFENTRVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA 300

Query: 301 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAIMTGTEFQANDLGLLIENTSIEQLG 360
           LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAI+TG EFQANDLGLL+ENTSIEQLG
Sbjct: 301 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQANDLGLLVENTSIEQLG 360

Query: 361 LARKVTISKDTTTIIADAASKDEIQARIAQIKKELAETDSVYDTEKLAERIAKLSGGVAV 420
           LARKVTISKDTTTIIADAASKDE+QARIAQ+KKELAETDSVYDTEKLAERIAKLSGGVAV
Sbjct: 361 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV 420

Query: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTYVPAIKDKLEDAEE 480
           IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLST VPAIKDKLEDAEE
Sbjct: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE 480

Query: 481 KLGADIIQKALVAPASLIAQNAGVEGEVVVEKIKASEWEIGYNAMTDKYENLVEAGVIDP 540
           KLGADI+QKALVAPASLIAQNAG+EGEVVVEKIK+SEWEIGYNAMTDKYENLVEAGVIDP
Sbjct: 481 KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP 540

Query: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPVAAPQGLTI 584
           AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAP AAPQGLTI
Sbjct: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTI 583

BLAST of MS005091 vs. ExPASy TrEMBL
Match: A0A4Y5WS72 (RuBisCO1 OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 1025.0 bits (2649), Expect = 1.2e-295
Identity = 554/583 (95.03%), Postives = 575/583 (98.63%), Query Frame = 0

Query: 1   MASANAISSASILCSSQKNLRKVNQSQSSRLNYRQAGSRFVVRATAKEIAFDQSSRAALQ 60
           MASANAISSASILCSSQK+LRKVNQ QS+R+NYRQAGSRFVVRATAK+IAFDQSSR ALQ
Sbjct: 1   MASANAISSASILCSSQKSLRKVNQPQSNRVNYRQAGSRFVVRATAKDIAFDQSSRTALQ 60

Query: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDAMENAGAALIREVA 120
           SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPD MENAGAALIREVA
Sbjct: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 120

Query: 121 SKTNDSAGDGTTTASVLAREIIKLGLLSVTSGANPVSLKKGIDKTVQGLIEELENKARAV 180
           SKTNDSAGDGTTTASVLAREIIKLGLL+VTSGANPVSLK+GIDKTVQGLIEELE KARA+
Sbjct: 121 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIEELEKKARAI 180

Query: 181 KGGDDIKAVASISAGNDELIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240
           +G DDIKAVASISAGNDELIGSMIADAI+KVGPDGVLSIESSSSFETTVEVEEGMAIDRG
Sbjct: 181 EGRDDIKAVASISAGNDELIGSMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240

Query: 241 YISPQFVTNPEKLIAEFENARVLVTDQKITAIKDIIPLLEKTTQLRAPLLIIAEDVTGEA 300
           YISPQFVTNPEKLIAEFENARVLVTDQKI+AIKDIIP+LEKTTQLRAPLLIIAED+TGEA
Sbjct: 241 YISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDITGEA 300

Query: 301 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAIMTGTEFQANDLGLLIENTSIEQLG 360
           LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAI+TG EFQA+DLGLL+ENT+IEQLG
Sbjct: 301 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQASDLGLLVENTTIEQLG 360

Query: 361 LARKVTISKDTTTIIADAASKDEIQARIAQIKKELAETDSVYDTEKLAERIAKLSGGVAV 420
           LARKVTISKDTTTIIADAASKDE+QARIAQ+KKELAETDSVYDTEKLAERIAKLSGGVAV
Sbjct: 361 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV 420

Query: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTYVPAIKDKLEDAEE 480
           IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGG ALVHLST VPAIKDKLEDAEE
Sbjct: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGTALVHLSTLVPAIKDKLEDAEE 480

Query: 481 KLGADIIQKALVAPASLIAQNAGVEGEVVVEKIKASEWEIGYNAMTDKYENLVEAGVIDP 540
           KLGADIIQKALVAPASLIAQNAGVEGEVVVEKIK+SEWEIGYNAMTDKYENLVEAGVIDP
Sbjct: 481 KLGADIIQKALVAPASLIAQNAGVEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP 540

Query: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPVAAPQGLTI 584
           AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAP+AAPQGLT+
Sbjct: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPIAAPQGLTV 583

BLAST of MS005091 vs. ExPASy TrEMBL
Match: A0A6J1FXR2 (ruBisCO large subunit-binding protein subunit alpha-like OS=Cucurbita moschata OX=3662 GN=LOC111448162 PE=3 SV=1)

HSP 1 Score: 1024.6 bits (2648), Expect = 1.6e-295
Identity = 553/583 (94.85%), Postives = 575/583 (98.63%), Query Frame = 0

Query: 1   MASANAISSASILCSSQKNLRKVNQSQSSRLNYRQAGSRFVVRATAKEIAFDQSSRAALQ 60
           MASANAISSASILCSSQK+LRK NQ+Q++RLNYRQAGSRFVVRATAKEIAFDQSSR+ALQ
Sbjct: 1   MASANAISSASILCSSQKSLRKANQTQNNRLNYRQAGSRFVVRATAKEIAFDQSSRSALQ 60

Query: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDAMENAGAALIREVA 120
           SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPD MENAGAALIREVA
Sbjct: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 120

Query: 121 SKTNDSAGDGTTTASVLAREIIKLGLLSVTSGANPVSLKKGIDKTVQGLIEELENKARAV 180
           SKTNDSAGDGTTTASVLAREIIKLGLL+VTSGANPVSLK+GIDKTVQGLI+ELENKARA+
Sbjct: 121 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIQELENKARAI 180

Query: 181 KGGDDIKAVASISAGNDELIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240
           +G DDIKAVASISAGNDELIG MIADAI+KVGPDGVLSIESSSSFETTVEVEEGMAIDRG
Sbjct: 181 EGRDDIKAVASISAGNDELIGMMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240

Query: 241 YISPQFVTNPEKLIAEFENARVLVTDQKITAIKDIIPLLEKTTQLRAPLLIIAEDVTGEA 300
           YISPQFVTNPEKLIAEFENARVLVTDQKI+AIKDIIP+LEKTTQLRAPLLIIAEDVTGEA
Sbjct: 241 YISPQFVTNPEKLIAEFENARVLVTDQKISAIKDIIPILEKTTQLRAPLLIIAEDVTGEA 300

Query: 301 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAIMTGTEFQANDLGLLIENTSIEQLG 360
           LATLVVNKLRGILNVAAIKAP FGERRKAMLQDIAI+TG EFQANDLGLL+ENT+IEQLG
Sbjct: 301 LATLVVNKLRGILNVAAIKAPSFGERRKAMLQDIAILTGAEFQANDLGLLVENTTIEQLG 360

Query: 361 LARKVTISKDTTTIIADAASKDEIQARIAQIKKELAETDSVYDTEKLAERIAKLSGGVAV 420
           LARKVTISKDTTTIIADAASKDE+QARIAQ+KKELAETDSVYDTEKLAERIAKLSGGVAV
Sbjct: 361 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLAERIAKLSGGVAV 420

Query: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTYVPAIKDKLEDAEE 480
           IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLST VPAIKDKLEDAEE
Sbjct: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTLVPAIKDKLEDAEE 480

Query: 481 KLGADIIQKALVAPASLIAQNAGVEGEVVVEKIKASEWEIGYNAMTDKYENLVEAGVIDP 540
           KLGADI+QKALVAPASLIAQNAG+EGEVVVEKIK+SEWEIGYNAMTDKYENLVEAGVIDP
Sbjct: 481 KLGADIVQKALVAPASLIAQNAGIEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP 540

Query: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPVAAPQGLTI 584
           AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAP AAPQGLT+
Sbjct: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPTAAPQGLTV 583

BLAST of MS005091 vs. ExPASy TrEMBL
Match: A0A6J1H2E5 (ruBisCO large subunit-binding protein subunit alpha OS=Cucurbita moschata OX=3662 GN=LOC111459478 PE=3 SV=1)

HSP 1 Score: 1023.5 bits (2645), Expect = 3.5e-295
Identity = 552/583 (94.68%), Postives = 575/583 (98.63%), Query Frame = 0

Query: 1   MASANAISSASILCSSQKNLRKVNQSQSSRLNYRQAGSRFVVRATAKEIAFDQSSRAALQ 60
           MASANAISSASILCSSQK+LRKVNQ QS+R+NYRQAGSRFVVRATAK+IAFDQSSR ALQ
Sbjct: 1   MASANAISSASILCSSQKSLRKVNQPQSNRVNYRQAGSRFVVRATAKDIAFDQSSRTALQ 60

Query: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDAMENAGAALIREVA 120
           SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPD MENAGAALIREVA
Sbjct: 61  SGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDPMENAGAALIREVA 120

Query: 121 SKTNDSAGDGTTTASVLAREIIKLGLLSVTSGANPVSLKKGIDKTVQGLIEELENKARAV 180
           SKTNDSAGDGTTTASVLAREIIKLGLL+VTSGANPVSLK+GIDKTVQGLIEELE KARA+
Sbjct: 121 SKTNDSAGDGTTTASVLAREIIKLGLLNVTSGANPVSLKRGIDKTVQGLIEELEKKARAI 180

Query: 181 KGGDDIKAVASISAGNDELIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240
           +G DDIKAVASISAGNDELIGSMIADAI+KVGPDGVLSIESSSSFETTVEVEEGMAIDRG
Sbjct: 181 EGRDDIKAVASISAGNDELIGSMIADAIEKVGPDGVLSIESSSSFETTVEVEEGMAIDRG 240

Query: 241 YISPQFVTNPEKLIAEFENARVLVTDQKITAIKDIIPLLEKTTQLRAPLLIIAEDVTGEA 300
           YISPQFVTNPEKLIAEFENARVL+TDQKI+AIKDIIP+LEKTTQLRAPLLIIAED+TGEA
Sbjct: 241 YISPQFVTNPEKLIAEFENARVLITDQKISAIKDIIPILEKTTQLRAPLLIIAEDITGEA 300

Query: 301 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAIMTGTEFQANDLGLLIENTSIEQLG 360
           LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAI+TG EFQA+DLGLL+ENT+IEQLG
Sbjct: 301 LATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAILTGAEFQASDLGLLVENTTIEQLG 360

Query: 361 LARKVTISKDTTTIIADAASKDEIQARIAQIKKELAETDSVYDTEKLAERIAKLSGGVAV 420
           LARKVTISKDTTTIIADAASKDE+QARIAQ+KKELAETDSVYDTEKL+ERIAKLSGGVAV
Sbjct: 361 LARKVTISKDTTTIIADAASKDELQARIAQLKKELAETDSVYDTEKLSERIAKLSGGVAV 420

Query: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTYVPAIKDKLEDAEE 480
           IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGG ALVHLST VPAIKDKLEDAEE
Sbjct: 421 IKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGTALVHLSTLVPAIKDKLEDAEE 480

Query: 481 KLGADIIQKALVAPASLIAQNAGVEGEVVVEKIKASEWEIGYNAMTDKYENLVEAGVIDP 540
           KLGADIIQKALVAPASLIAQNAGVEGEVVVEKIK+SEWEIGYNAMTDKYENLVEAGVIDP
Sbjct: 481 KLGADIIQKALVAPASLIAQNAGVEGEVVVEKIKSSEWEIGYNAMTDKYENLVEAGVIDP 540

Query: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPVAAPQGLTI 584
           AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAP+AAPQGLT+
Sbjct: 541 AKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPIAAPQGLTV 583

BLAST of MS005091 vs. TAIR 10
Match: AT2G28000.1 (chaperonin-60alpha )

HSP 1 Score: 923.3 bits (2385), Expect = 9.6e-269
Identity = 502/586 (85.67%), Postives = 545/586 (93.00%), Query Frame = 0

Query: 1   MASANAISSASILCSS-QKNLRKVNQSQSSRLNY-RQAGSRFVVRATAKEIAFDQSSRAA 60
           MASANA+SSAS+LCSS Q  L   NQ Q  R++Y ++   RF VRA  KEIAFDQ SRAA
Sbjct: 1   MASANALSSASVLCSSRQSKLGGGNQQQGQRVSYNKRTIRRFSVRANVKEIAFDQHSRAA 60

Query: 61  LQSGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPDAMENAGAALIRE 120
           LQ+GIDKLA+ VGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELP+AMENAGAALIRE
Sbjct: 61  LQAGIDKLADCVGLTLGPRGRNVVLDEFGSPKVVNDGVTIARAIELPNAMENAGAALIRE 120

Query: 121 VASKTNDSAGDGTTTASVLAREIIKLGLLSVTSGANPVSLKKGIDKTVQGLIEELENKAR 180
           VASKTNDSAGDGTTTAS+LAREIIK GLLSVTSGANPVSLK+GIDKTVQGLIEEL+ KAR
Sbjct: 121 VASKTNDSAGDGTTTASILAREIIKHGLLSVTSGANPVSLKRGIDKTVQGLIEELQKKAR 180

Query: 181 AVKGGDDIKAVASISAGNDELIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMAID 240
            VKG DDI+AVASISAGND+LIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGM ID
Sbjct: 181 PVKGRDDIRAVASISAGNDDLIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMEID 240

Query: 241 RGYISPQFVTNPEKLIAEFENARVLVTDQKITAIKDIIPLLEKTTQLRAPLLIIAEDVTG 300
           RGYISPQFVTNPEKL+AEFENARVL+TDQKITAIKDIIP+LEKTTQLRAPLLIIAEDVTG
Sbjct: 241 RGYISPQFVTNPEKLLAEFENARVLITDQKITAIKDIIPILEKTTQLRAPLLIIAEDVTG 300

Query: 301 EALATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAIMTGTEFQANDLGLLIENTSIEQ 360
           EALATLVVNKLRG+LNV A+KAPGFGERRKAMLQDIAI+TG E+ A D+ LL+EN +I+Q
Sbjct: 301 EALATLVVNKLRGVLNVVAVKAPGFGERRKAMLQDIAILTGAEYLAMDMSLLVENATIDQ 360

Query: 361 LGLARKVTISKDTTTIIADAASKDEIQARIAQIKKELAETDSVYDTEKLAERIAKLSGGV 420
           LG+ARKVTISKD+TT+IADAASKDE+QARIAQ+KKEL ETDSVYD+EKLAERIAKLSGGV
Sbjct: 361 LGIARKVTISKDSTTLIADAASKDELQARIAQLKKELFETDSVYDSEKLAERIAKLSGGV 420

Query: 421 AVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTYVPAIKDKLEDA 480
           AVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLST +PAIK+  EDA
Sbjct: 421 AVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTVIPAIKETFEDA 480

Query: 481 EEKLGADIIQKALVAPASLIAQNAGVEGEVVVEKIKASEWEIGYNAMTDKYENLVEAGVI 540
           +E+LGADI+QKAL++PA+LIAQNAGVEGEVVVEKI  S+WE GYNAMTD YENL EAGVI
Sbjct: 481 DERLGADIVQKALLSPAALIAQNAGVEGEVVVEKIMFSDWENGYNAMTDTYENLFEAGVI 540

Query: 541 DPAKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAP-VAAPQGLTI 584
           DPAKVTRCALQNAASVAGMVLTTQAIVV+KPKPKAP  AAP+GL +
Sbjct: 541 DPAKVTRCALQNAASVAGMVLTTQAIVVDKPKPKAPAAAAPEGLMV 586

BLAST of MS005091 vs. TAIR 10
Match: AT5G18820.1 (TCP-1/cpn60 chaperonin family protein )

HSP 1 Score: 636.3 bits (1640), Expect = 2.3e-182
Identity = 335/538 (62.27%), Postives = 434/538 (80.67%), Query Frame = 0

Query: 41  VVRATAKEIAFDQSSRAALQSGIDKLANAVGLTLGPRGRNVVLDEFGSPKVVNDGVTIAR 100
           VVRA AK I + + SR  LQ+GIDKLA+AV +TLGPRGRNVVL E  + KV+NDGVTIA+
Sbjct: 30  VVRAGAKRILYGKDSREKLQAGIDKLADAVSITLGPRGRNVVLAEKDTIKVINDGVTIAK 89

Query: 101 AIELPDAMENAGAALIREVASKTNDSAGDGTTTASVLAREIIKLGLLSVTSGANPVSLKK 160
           +IELPD +ENAGA LI+EVA K N+SAGDGTTTA +LARE+IK G L++  GAN VS+K 
Sbjct: 90  SIELPDTIENAGATLIQEVAIKMNESAGDGTTTAIILAREMIKAGSLAIAFGANAVSVKN 149

Query: 161 GIDKTVQGLIEELENKARAVKGGDDIKAVASISAGNDELIGSMIADAIDKVGPDGVLSIE 220
           G++KTV+ L+  L+ K+  V+G +DIKAVASISAGNDE +G++IA+ ++K+GPDGV+SIE
Sbjct: 150 GMNKTVKELVRVLQMKSIPVQGKNDIKAVASISAGNDEFVGNLIAETVEKIGPDGVISIE 209

Query: 221 SSSSFETTVEVEEGMAIDRGYISPQFVTNPEKLIAEFENARVLVTDQKITAIKDIIPLLE 280
           SSS+ ET+V VEEGM  D+GY+SP F+TN EK   EF+ A++LVTDQKIT+ K+++PLLE
Sbjct: 210 SSSTSETSVIVEEGMKFDKGYMSPHFITNQEKSTVEFDKAKILVTDQKITSAKELVPLLE 269

Query: 281 KTTQLRAPLLIIAEDVTGEALATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAIMTGT 340
           KT+QL  PLLIIAED++ E L  LVVNK +G++NVA +K PG  + +KA+LQDIA+MTG 
Sbjct: 270 KTSQLSVPLLIIAEDISAEVLEILVVNKKQGLINVAVVKCPGMLDGKKALLQDIALMTGA 329

Query: 341 EFQANDLGLLIENTSIEQLGLARKVTISKDTTTIIADAASKDEIQARIAQIKKELAETDS 400
           ++ + DLG+ +   + +QLG++R+V I+ ++TTI+ADA++K EIQARIAQ+KK+LAETD+
Sbjct: 330 DYLSGDLGMSLMGATSDQLGVSRRVVITANSTTIVADASTKPEIQARIAQMKKDLAETDN 389

Query: 401 VYDTEKLAERIAKLSGGVAVIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAA 460
            Y ++K+AERIAKL+GGVAVIKVG  TETELEDRKLRIEDAKNATFAA+ EGIVPGGGA 
Sbjct: 390 SYLSKKIAERIAKLTGGVAVIKVGGHTETELEDRKLRIEDAKNATFAAMREGIVPGGGAT 449

Query: 461 LVHLSTYVPAIKDKL-EDAEEKLGADIIQKALVAPASLIAQNAGVEGEVVVEKIKASEWE 520
            +HL   +P IK  L ED+ E++GADI+  AL APA  IA NAGV+G VVV+K +  EW 
Sbjct: 450 YIHLLDEIPRIKKNLMEDSYEQIGADIVAMALTAPAMAIATNAGVDGSVVVQKTRELEWR 509

Query: 521 IGYNAMTDKYENLVEAGVIDPAKVTRCALQNAASVAGMVLTTQAIVVEK---PKPKAP 575
            GYNAM+ KYE+L+ AG+ DP +V+R ALQNA SVAG++LTTQA++VEK   PKP  P
Sbjct: 510 SGYNAMSGKYEDLLNAGIADPCRVSRFALQNAVSVAGIILTTQAVLVEKIKQPKPAVP 567

BLAST of MS005091 vs. TAIR 10
Match: AT3G13470.1 (TCP-1/cpn60 chaperonin family protein )

HSP 1 Score: 510.8 bits (1314), Expect = 1.5e-144
Identity = 292/579 (50.43%), Postives = 409/579 (70.64%), Query Frame = 0

Query: 3   SANAISSASILCSSQKNLRKVNQSQSSRLNYRQAGSRFVVRATAKEIAF--DQSSRAALQ 62
           +A  +SSA+ + SS         S   R N     SR  +   AKE+ F  D ++   LQ
Sbjct: 18  NAIKLSSATSISSS---------SFGRRHNVCVRRSRPAIVCAAKELHFNKDGTTIRKLQ 77

Query: 63  SGIDKLANAVGLTLGPRGRNVVLD-EFGSPKVVNDGVTIARAIELPDAMENAGAALIREV 122
           +G++KLA+ VG+TLGP+GRNVVL+ ++GSP++VNDGVT+AR +EL D +EN GA L+R+ 
Sbjct: 78  TGVNKLADLVGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVELEDPVENIGAKLVRQA 137

Query: 123 ASKTNDSAGDGTTTASVLAREIIKLGLLSVTSGANPVSLKKGIDKTVQGLIEELENKARA 182
           A+KTND AGDGTTT+ VLA+  I  G+  V +GANPV + +GI+KT + L+ EL+  ++ 
Sbjct: 138 AAKTNDLAGDGTTTSVVLAQGFIAEGVKVVAAGANPVLITRGIEKTAKALVNELKLMSKE 197

Query: 183 VKGGDDIKAVASISAGNDELIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMAIDR 242
           V+   ++  VA++SAGN+  +GSMIA+A+ KVG  GV+++E   S E  + V EGM  DR
Sbjct: 198 VE-DSELADVAAVSAGNNHEVGSMIAEAMSKVGRKGVVTLEEGKSAENNLYVVEGMQFDR 257

Query: 243 GYISPQFVTNPEKLIAEFENARVLVTDQKITAIKDIIPLLEKTTQLRAPLLIIAEDVTGE 302
           GYISP FVT+ EK+  E++N ++L+ D+K+T  +D++ +LE   +   P+LIIAED+  E
Sbjct: 258 GYISPYFVTDSEKMSVEYDNCKLLLVDKKVTNARDLVGVLEDAIRGGYPILIIAEDIEQE 317

Query: 303 ALATLVVNKLRGILNVAAIKAPGFGERRKAMLQDIAIMTGTEFQANDLGLLIENTSIEQL 362
           ALATLVVNKLRG L +AA+KAPGFGER+   L DIAI+TG      ++GL ++    E L
Sbjct: 318 ALATLVVNKLRGTLKIAALKAPGFGERKSQYLDDIAILTGATVIREEVGLSLDKAGKEVL 377

Query: 363 GLARKVTISKDTTTIIADAASKDEIQARIAQIKKELAETDSVYDTEKLAERIAKLSGGVA 422
           G A KV ++K+ TTI+ D  +++ +  R+ QI+  + + +  Y+ EKL ERIAKLSGGVA
Sbjct: 378 GNASKVVLTKEMTTIVGDGTTQEAVNKRVVQIRNLIEQAEQDYEKEKLNERIAKLSGGVA 437

Query: 423 VIKVGAATETELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTYVPAIKDKLEDAE 482
           VI+VGA TETEL+++KLR+EDA NAT AA+EEGIV GGG  L+ L++ V AIKD LE+ E
Sbjct: 438 VIQVGAQTETELKEKKLRVEDALNATKAAVEEGIVVGGGCTLLRLASKVDAIKDTLENDE 497

Query: 483 EKLGADIIQKALVAPASLIAQNAGVEGEVVVEKIKASE-WEIGYNAMTDKYENLVEAGVI 542
           EK+GA+I+++AL  P  LIA+NAGV G VV EK+ A++  + GYNA T KYE+L+ AG+I
Sbjct: 498 EKVGAEIVKRALSYPLKLIAKNAGVNGSVVSEKVLANDNVKFGYNAATGKYEDLMAAGII 557

Query: 543 DPAKVTRCALQNAASVAGMVLTTQAIVVEKPKPKAPVAA 578
           DP KV RC L++AASVA   L +  +VVE P+P+ PV A
Sbjct: 558 DPTKVVRCCLEHAASVAKTFLMSDCVVVEIPEPE-PVPA 585

BLAST of MS005091 vs. TAIR 10
Match: AT1G55490.1 (chaperonin 60 beta )

HSP 1 Score: 510.4 bits (1313), Expect = 1.9e-144
Identity = 288/572 (50.35%), Postives = 407/572 (71.15%), Query Frame = 0

Query: 15  SSQKNLRKVNQSQSSRLNY---RQAGSRFVVRATAKEIAF--DQSSRAALQSGIDKLANA 74
           S +K + K++ S   R      R   S   +   AKE+ F  D ++   LQ+G++KLA+ 
Sbjct: 22  SDKKLISKLSSSSFGRRQSVCPRPRRSSSAIVCAAKELHFNKDGTTIRRLQAGVNKLADL 81

Query: 75  VGLTLGPRGRNVVLD-EFGSPKVVNDGVTIARAIELPDAMENAGAALIREVASKTNDSAG 134
           VG+TLGP+GRNVVL+ ++GSP++VNDGVT+AR +EL D +EN GA L+R+ A+KTND AG
Sbjct: 82  VGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVELEDPVENIGAKLVRQAAAKTNDLAG 141

Query: 135 DGTTTASVLAREIIKLGLLSVTSGANPVSLKKGIDKTVQGLIEELENKARAVKGGDDIKA 194
           DGTTT+ VLA+  I  G+  V +GANPV + +GI+KT + L+ EL+  ++ V+   ++  
Sbjct: 142 DGTTTSVVLAQGFIAEGVKVVAAGANPVLITRGIEKTAKALVTELKKMSKEVE-DSELAD 201

Query: 195 VASISAGNDELIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMAIDRGYISPQFVT 254
           VA++SAGN++ IG+MIA+A+ KVG  GV+++E   S E  + V EGM  DRGYISP FVT
Sbjct: 202 VAAVSAGNNDEIGNMIAEAMSKVGRKGVVTLEEGKSAENNLYVVEGMQFDRGYISPYFVT 261

Query: 255 NPEKLIAEFENARVLVTDQKITAIKDIIPLLEKTTQLRAPLLIIAEDVTGEALATLVVNK 314
           + EK+  EF+N ++L+ D+KIT  +D++ +LE   +   P+LIIAED+  EALATLVVNK
Sbjct: 262 DSEKMSVEFDNCKLLLVDKKITNARDLVGVLEDAIRGGYPILIIAEDIEQEALATLVVNK 321

Query: 315 LRGILNVAAIKAPGFGERRKAMLQDIAIMTGTEFQANDLGLLIENTSIEQLGLARKVTIS 374
           LRG L +AA++APGFGER+   L DIAI+TG      ++GL ++    E LG A KV ++
Sbjct: 322 LRGTLKIAALRAPGFGERKSQYLDDIAILTGATVIREEVGLSLDKAGKEVLGNASKVVLT 381

Query: 375 KDTTTIIADAASKDEIQARIAQIKKELAETDSVYDTEKLAERIAKLSGGVAVIKVGAATE 434
           K+T+TI+ D +++D ++ R+ QIK  + + +  Y+ EKL ERIAKLSGGVAVI+VGA TE
Sbjct: 382 KETSTIVGDGSTQDAVKKRVTQIKNLIEQAEQDYEKEKLNERIAKLSGGVAVIQVGAQTE 441

Query: 435 TELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTYVPAIKDKLEDAEEKLGADIIQ 494
           TEL+++KLR+EDA NAT AA+EEGIV GGG  L+ L++ V AIK  L++ EEK+GADI++
Sbjct: 442 TELKEKKLRVEDALNATKAAVEEGIVVGGGCTLLRLASKVDAIKATLDNDEEKVGADIVK 501

Query: 495 KALVAPASLIAQNAGVEGEVVVEKIKASE-WEIGYNAMTDKYENLVEAGVIDPAKVTRCA 554
           +AL  P  LIA+NAGV G VV EK+ +++  + GYNA T KYE+L+ AG+IDP KV RC 
Sbjct: 502 RALSYPLKLIAKNAGVNGSVVSEKVLSNDNVKFGYNAATGKYEDLMAAGIIDPTKVVRCC 561

Query: 555 LQNAASVAGMVLTTQAIVVEKPKPK-APVAAP 579
           L++AASVA   L +  +VVE  +P+  PV  P
Sbjct: 562 LEHAASVAKTFLMSDCVVVEIKEPEPVPVGNP 592

BLAST of MS005091 vs. TAIR 10
Match: AT1G55490.2 (chaperonin 60 beta )

HSP 1 Score: 510.4 bits (1313), Expect = 1.9e-144
Identity = 288/572 (50.35%), Postives = 407/572 (71.15%), Query Frame = 0

Query: 15  SSQKNLRKVNQSQSSRLNY---RQAGSRFVVRATAKEIAF--DQSSRAALQSGIDKLANA 74
           S +K + K++ S   R      R   S   +   AKE+ F  D ++   LQ+G++KLA+ 
Sbjct: 22  SDKKLISKLSSSSFGRRQSVCPRPRRSSSAIVCAAKELHFNKDGTTIRRLQAGVNKLADL 81

Query: 75  VGLTLGPRGRNVVLD-EFGSPKVVNDGVTIARAIELPDAMENAGAALIREVASKTNDSAG 134
           VG+TLGP+GRNVVL+ ++GSP++VNDGVT+AR +EL D +EN GA L+R+ A+KTND AG
Sbjct: 82  VGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVELEDPVENIGAKLVRQAAAKTNDLAG 141

Query: 135 DGTTTASVLAREIIKLGLLSVTSGANPVSLKKGIDKTVQGLIEELENKARAVKGGDDIKA 194
           DGTTT+ VLA+  I  G+  V +GANPV + +GI+KT + L+ EL+  ++ V+   ++  
Sbjct: 142 DGTTTSVVLAQGFIAEGVKVVAAGANPVLITRGIEKTAKALVTELKKMSKEVE-DSELAD 201

Query: 195 VASISAGNDELIGSMIADAIDKVGPDGVLSIESSSSFETTVEVEEGMAIDRGYISPQFVT 254
           VA++SAGN++ IG+MIA+A+ KVG  GV+++E   S E  + V EGM  DRGYISP FVT
Sbjct: 202 VAAVSAGNNDEIGNMIAEAMSKVGRKGVVTLEEGKSAENNLYVVEGMQFDRGYISPYFVT 261

Query: 255 NPEKLIAEFENARVLVTDQKITAIKDIIPLLEKTTQLRAPLLIIAEDVTGEALATLVVNK 314
           + EK+  EF+N ++L+ D+KIT  +D++ +LE   +   P+LIIAED+  EALATLVVNK
Sbjct: 262 DSEKMSVEFDNCKLLLVDKKITNARDLVGVLEDAIRGGYPILIIAEDIEQEALATLVVNK 321

Query: 315 LRGILNVAAIKAPGFGERRKAMLQDIAIMTGTEFQANDLGLLIENTSIEQLGLARKVTIS 374
           LRG L +AA++APGFGER+   L DIAI+TG      ++GL ++    E LG A KV ++
Sbjct: 322 LRGTLKIAALRAPGFGERKSQYLDDIAILTGATVIREEVGLSLDKAGKEVLGNASKVVLT 381

Query: 375 KDTTTIIADAASKDEIQARIAQIKKELAETDSVYDTEKLAERIAKLSGGVAVIKVGAATE 434
           K+T+TI+ D +++D ++ R+ QIK  + + +  Y+ EKL ERIAKLSGGVAVI+VGA TE
Sbjct: 382 KETSTIVGDGSTQDAVKKRVTQIKNLIEQAEQDYEKEKLNERIAKLSGGVAVIQVGAQTE 441

Query: 435 TELEDRKLRIEDAKNATFAAIEEGIVPGGGAALVHLSTYVPAIKDKLEDAEEKLGADIIQ 494
           TEL+++KLR+EDA NAT AA+EEGIV GGG  L+ L++ V AIK  L++ EEK+GADI++
Sbjct: 442 TELKEKKLRVEDALNATKAAVEEGIVVGGGCTLLRLASKVDAIKATLDNDEEKVGADIVK 501

Query: 495 KALVAPASLIAQNAGVEGEVVVEKIKASE-WEIGYNAMTDKYENLVEAGVIDPAKVTRCA 554
           +AL  P  LIA+NAGV G VV EK+ +++  + GYNA T KYE+L+ AG+IDP KV RC 
Sbjct: 502 RALSYPLKLIAKNAGVNGSVVSEKVLSNDNVKFGYNAATGKYEDLMAAGIIDPTKVVRCC 561

Query: 555 LQNAASVAGMVLTTQAIVVEKPKPK-APVAAP 579
           L++AASVA   L +  +VVE  +P+  PV  P
Sbjct: 562 LEHAASVAKTFLMSDCVVVEIKEPEPVPVGNP 592

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140442.11.4e-30699.83ruBisCO large subunit-binding protein subunit alpha [Momordica charantia][more]
XP_022985565.16.6e-29695.20ruBisCO large subunit-binding protein subunit alpha-like isoform X1 [Cucurbita m... [more]
KAG6570801.12.5e-29595.03hypothetical protein SDJN03_29716, partial [Cucurbita argyrosperma subsp. sorori... [more]
QDE10505.12.5e-29595.03ruBisCO1 [Cucurbita pepo][more]
XP_022943380.13.3e-29594.85ruBisCO large subunit-binding protein subunit alpha-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
P089267.7e-27186.22RuBisCO large subunit-binding protein subunit alpha, chloroplastic OS=Pisum sati... [more]
P212381.4e-26785.67Chaperonin 60 subunit alpha 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=... [more]
P212391.3e-26589.19RuBisCO large subunit-binding protein subunit alpha, chloroplastic (Fragment) OS... [more]
P347941.2e-26084.44RuBisCO large subunit-binding protein subunit alpha, chloroplastic OS=Brassica n... [more]
P088236.7e-25185.98RuBisCO large subunit-binding protein subunit alpha, chloroplastic (Fragment) OS... [more]
Match NameE-valueIdentityDescription
A0A6J1CGX66.9e-30799.83ruBisCO large subunit-binding protein subunit alpha OS=Momordica charantia OX=36... [more]
A0A6J1JBN63.2e-29695.20ruBisCO large subunit-binding protein subunit alpha-like isoform X1 OS=Cucurbita... [more]
A0A4Y5WS721.2e-29595.03RuBisCO1 OS=Cucurbita pepo OX=3663 PE=2 SV=1[more]
A0A6J1FXR21.6e-29594.85ruBisCO large subunit-binding protein subunit alpha-like OS=Cucurbita moschata O... [more]
A0A6J1H2E53.5e-29594.68ruBisCO large subunit-binding protein subunit alpha OS=Cucurbita moschata OX=366... [more]
Match NameE-valueIdentityDescription
AT2G28000.19.6e-26985.67chaperonin-60alpha [more]
AT5G18820.12.3e-18262.27TCP-1/cpn60 chaperonin family protein [more]
AT3G13470.11.5e-14450.43TCP-1/cpn60 chaperonin family protein [more]
AT1G55490.11.9e-14450.35chaperonin 60 beta [more]
AT1G55490.21.9e-14450.35chaperonin 60 beta [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 381..401
NoneNo IPR availablePANTHERPTHR4563360 KDA HEAT SHOCK PROTEIN, MITOCHONDRIALcoord: 1..578
NoneNo IPR availablePANTHERPTHR45633:SF39BNAA04G16190D PROTEINcoord: 1..578
IPR001844Chaperonin Cpn60PRINTSPR00298CHAPERONIN60coord: 70..96
score: 40.38
coord: 125..152
score: 58.02
coord: 440..461
score: 73.3
coord: 392..417
score: 57.23
coord: 310..333
score: 71.1
IPR001844Chaperonin Cpn60TIGRFAMTIGR02348TIGR02348coord: 46..570
e-value: 2.7E-221
score: 733.6
IPR001844Chaperonin Cpn60HAMAPMF_00600CH60coord: 45..581
score: 36.486477
IPR001844Chaperonin Cpn60CDDcd03344GroELcoord: 47..567
e-value: 0.0
score: 807.45
IPR002423Chaperonin Cpn60/TCP-1 familyPFAMPF00118Cpn60_TCP1coord: 66..569
e-value: 1.9E-94
score: 317.1
IPR027413GroEL-like equatorial domain superfamilyGENE3D1.10.560.10coord: 49..566
e-value: 5.9E-212
score: 706.6
IPR027413GroEL-like equatorial domain superfamilySUPERFAMILY48592GroEL equatorial domain-likecoord: 55..565
IPR027409GroEL-like apical domain superfamilyGENE3D3.50.7.10GroELcoord: 234..415
e-value: 5.9E-212
score: 706.6
IPR027409GroEL-like apical domain superfamilySUPERFAMILY52029GroEL apical domain-likecoord: 226..418
IPR027410TCP-1-like chaperonin intermediate domain superfamilyGENE3D3.30.260.10coord: 179..452
e-value: 5.9E-212
score: 706.6
IPR027410TCP-1-like chaperonin intermediate domain superfamilySUPERFAMILY54849GroEL-intermediate domain likecoord: 180..243
IPR027410TCP-1-like chaperonin intermediate domain superfamilySUPERFAMILY54849GroEL-intermediate domain likecoord: 402..450
IPR018370Chaperonin Cpn60, conserved sitePROSITEPS00296CHAPERONINS_CPN60coord: 447..458

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS005091.1MS005091.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006457 protein folding
biological_process GO:0042026 protein refolding
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity