MS005001 (gene) Bitter gourd (TR) v1

Overview
NameMS005001
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionisocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like
Locationscaffold176: 2015443 .. 2019222 (-)
RNA-Seq ExpressionMS005001
SyntenyMS005001
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GCCGCCCGTCGAGCCTTTCCGATCATTCGCCACCTCACCTCCCCCTGCCGGAGCCGTTCTCGTATCCTCCCTCTCCGCTCCGACACCCGATCCGTCACCTACATGCCGAGGCCCGGCGAGGGATCCCCGCGTCAGGTGACTCTAATTCCCGGAGACGGAATCGGTCCTCTGGTGACCAACGCGGTCGAGCAAGTGATGGAGGCAATGCACGCTCCCGTCTACTTCGAGCGCTACGAAGTCCATGGCGATATGAAGCAGGTTCCTCGAGAGGTGATGGAGTCTATTTTGAAGAATAAGGTGTGTTTGAAAGGCGGGCTGATTACTCCGATGGGCGGCGGTGTGAGTTCACTGAATGTGCAGCTCAGGAAGGAGCTCGACTTGTACGCCTCGCTTGTTAATTGCTTCAATTTCCCCGGATTTCCGACGCGTCATGAGAATGTCGACATCGTCGTCATTAGGGAGAATACGGAGGGCGAATACTCTGGCCTCGAGCATGAGGTCATCCCTGGCGTCGTCGAAAGCCTCAAGGTAATATATAATTTTGTTTGTGTCTGGTCTGGTGATCTTCTGGTTTTTTTCACTCTCCGTTTTGAGTTGATATAATTGATTGTTGATATAGTTTAGAATTTTATGTTTTATGGCTCTGTTTCTGATCCTATGATTGAGAGAAATTTTCTCTGTTTCATTCTTTAAGTGTTATGAACTTATGCACATCACCACTCTTGAAATGAACGAAACAAACAAAGCTATGAAACAACTGCCGCATTAACATTAATTTTTTAGTCGTTTAGTTTAGTCTTTTGTAGGTTATCTGTTATGAGATCAATTTCATATATCGGTATTCTAGTCAATGTATATGAAATCCATTCCGAGCAGATTTCAAGCAAATATATAAACTCTTGTGCTCAATGGGGTCATACTCTTAATCTAGTCAAAACCTGCTTTTCTTAGTCCCCATTTCAACGCAGTAACCTGAAATATAGCCTGAATTACTGATAGGTGATTACAAAGTTTTCCTCAGAACGCATTGCCAAATATGCATTTGAGTATGCTTATCTGAACAACAGAAAAAAAGTGACGGCGGTGCACAAAGCCAACATTATGAAACTTGCAGATGGTCTGTTTTTGGAGTCTTGCAGGGAGGTGGCAGCGCAATATCCTAGTATCCAATACAATGAGATTATTGTTGACAACTGTTGTATGCAGCTTGTTTCAAAACCTGAGCAATTTGATGTAATGGTACGTAACTTCCTAAGCTGATAATTTTTCCATTCAGGGTACTTTTTTGTTTTTCTCTTTTTGTTGTTGTCTTTTTTCCCACCACATGCTAGAATGAAAACAATATCCATTTTCTGTGGCCCTCTCATTGTTATGAGCTTCATATAGGCTGTATACTTCAGCTCTGTTTTCTGTCTTCATACTTTTGGAACTTGAATCAAGGGGCAATATGGTTCAAGCTTACAGTGCCTGCAGATTACTTTCTTGAACATGAATTTAAATGGCGTGTATAAACCAGTCCTCGATATGCCAGGCATGTCGATCTAAAGTGATTGGAGCCCTAGGACACACCACGGTGGTTATAAAAGAACCTTGGTTTCAACCAAGAGGTTTTGAGTGAAAATCTCTAAAGCCATCTTACCGGGGATATTAATTCTCTGGCACTTTTCTTGATTGATTTTATATGGTCATACAGCGTTTCTTAGTTTTGAAGGAAACTTGAGTCAAAATCCCCAAAGGCAAATTGTCGAGGGATATTAGTTCTCTTGCTAGTTTTTTGATTGATTTTATGGTTACATGGTTCTAAGTAAACAAACTTCTATGCACTGAAAGATGAGAACGATGGAAATTAAACAAACTTCCTGAAAAGAATTAGCAGTGGCATCGTTTGAACAGAGAAACGCAAAAATGTTGGTGGTTCAGTTGTGCAGATTAATTTGGCTCACTTTTTGTAGCCGTATCATATTACTTTGAAATGAAAGACAAATAATGGGGGCACATATATAGCCACGCATACTTGAAGGATAGGTGTATCAATTATGTTCCTCAAGGTTTTGCTTAATGCTTCTTGTTGATTTATTTGCTTATTTCTTAAGAAATCACGTATACAGATATGAGACACAAATATGACACGATACGGATATGATGACACGGTGTTTTCTAAAAATCTAGGACACAAAAACGTTAAAAGACACATTTATTAAAATATACAATTTTTTTTAAATATATATCATTTTTATACAAGTAGGAAATCAAAGTCAATAAGTTTATGTAGTTTCGTACTTTAAAAAAAATGAGTTTTATGTATTTTGCTCTCAAAATTTATGATTTTGTCCTATGTTACTATTTATATCTATTTCTTATACTTAAAAAGTGTTTGATACATGCCTATAACAAGTTTTGAAATCATATTCACTTTGTACAACTAATGTGTGATACACATCTATTGAGCTAACTAATGTCCGATACGTGTCTAATAAAGTGTCCTATACGGACACGTTTCCCAAATTGAAGTGTCTATGCTTCTTAGCTTCTTTCTTTACCTCTATGTTTTTTCTTGATTTCCCAGAATAAGTTCACTCTCCTTTCTTAAATATTCATCTTTTTAGTTTTTCCTTGATGCTTAAATTTTGCTTGTTATTTTTTAACCCAATGCCTTTTTATGCTTAAATGTTGCTAACAAGGCCTTTTTATTCTTGGGCAGGTGACTCCTAACCTTTATGGCAATCTAGTGGCCAACACTGCAGCTGGAATTGCTGGGGGTACTGGTCTCATGCCAGGAGGTAATATTATAATCCACTATGGCCTTTTAAAGTCCGTGCATTTGTGATGATAAATGCATTATGTGGTAATCGGTGCTGTTACCATTTTACTTAAATTTCTACACTAACTAACATGTAAAATGTCTAAGTTCTCAATTTTACTGAGAGATAATGAAAATGTCAATAAAATAATGGAATGTTGACAGATATTCTAGACAATTTCTGAAAATAAAAAAATTCCTAAAAACTATTTGAATTAATTAATGAACATTTTATATTTTTAAAATAACTTTAAATTCCTTAGTTATATTTTAGGCTAAATATATACTTAGTCCCTTGTTTTTGGGTATTTTTTTCAATTTAGTCCCCGACTTTTTTGGAGTTTCAATTTCATTATTTATGTATTGATTTTTACATTTAAGTCCTCGTTGTCACAAAAGGTTAAAATTAACTTATAAATTGATGATATATTGTCAAAATACATTTTATTAACTGAGTTGAAATTACTCAGGTCATGTTTGATCCTAACCCTTTTGTTTTTCGTTGTATGTTTTTTAAAATTAAGCCTTAAATTGCTTTCTATTCTAGAATTTGACTTGCTTAAATGTTGCCATACTAACCATTAAAAAGCTCATAAAAATTTCCATTTGGAAACTCATATTTCAATCCACTTTGATATCAATATTGAAGTTTTGGATATATATTCCTTGGTAGATGTTTTGAAATTGTGATTATAATGTGCACTGCAGGGAATGTGGGGTCAGATCATGCGATATTTGAGCAAGGAGCTTCAGCAGGGAACGTGGGGAATGAGAAAATAGTGGAGCAGAAGAAGGCAAACCCGGTGGCTTTGCTGCTGTCATCGGCCATGATGCTGAGGCACCTTCAGTTCCCTTCCTTCGCTGACAGACTCGAGAATGCTGTCAAAGCTGTCATCTTGGAGGCCAAGTGCAGGACCAAGGATCTTGGTGGAGACTGCACCACCCAAGATGTTGTCGATGCTGTCATTTCCCTTTTGGAC

mRNA sequence

GCCGCCCGTCGAGCCTTTCCGATCATTCGCCACCTCACCTCCCCCTGCCGGAGCCGTTCTCGTATCCTCCCTCTCCGCTCCGACACCCGATCCGTCACCTACATGCCGAGGCCCGGCGAGGGATCCCCGCGTCAGGTGACTCTAATTCCCGGAGACGGAATCGGTCCTCTGGTGACCAACGCGGTCGAGCAAGTGATGGAGGCAATGCACGCTCCCGTCTACTTCGAGCGCTACGAAGTCCATGGCGATATGAAGCAGGTTCCTCGAGAGGTGATGGAGTCTATTTTGAAGAATAAGGTGTGTTTGAAAGGCGGGCTGATTACTCCGATGGGCGGCGGTGTGAGTTCACTGAATGTGCAGCTCAGGAAGGAGCTCGACTTGTACGCCTCGCTTGTTAATTGCTTCAATTTCCCCGGATTTCCGACGCGTCATGAGAATGTCGACATCGTCGTCATTAGGGAGAATACGGAGGGCGAATACTCTGGCCTCGAGCATGAGGTCATCCCTGGCGTCGTCGAAAGCCTCAAGGTGATTACAAAGTTTTCCTCAGAACGCATTGCCAAATATGCATTTGAGTATGCTTATCTGAACAACAGAAAAAAAGTGACGGCGGTGCACAAAGCCAACATTATGAAACTTGCAGATGGTCTGTTTTTGGAGTCTTGCAGGGAGGTGGCAGCGCAATATCCTAGTATCCAATACAATGAGATTATTGTTGACAACTGTTGTATGCAGCTTGTTTCAAAACCTGAGCAATTTGATGTAATGGTGACTCCTAACCTTTATGGCAATCTAGTGGCCAACACTGCAGCTGGAATTGCTGGGGGTACTGGTCTCATGCCAGGAGGGAATGTGGGGTCAGATCATGCGATATTTGAGCAAGGAGCTTCAGCAGGGAACGTGGGGAATGAGAAAATAGTGGAGCAGAAGAAGGCAAACCCGGTGGCTTTGCTGCTGTCATCGGCCATGATGCTGAGGCACCTTCAGTTCCCTTCCTTCGCTGACAGACTCGAGAATGCTGTCAAAGCTGTCATCTTGGAGGCCAAGTGCAGGACCAAGGATCTTGGTGGAGACTGCACCACCCAAGATGTTGTCGATGCTGTCATTTCCCTTTTGGAC

Coding sequence (CDS)

GCCGCCCGTCGAGCCTTTCCGATCATTCGCCACCTCACCTCCCCCTGCCGGAGCCGTTCTCGTATCCTCCCTCTCCGCTCCGACACCCGATCCGTCACCTACATGCCGAGGCCCGGCGAGGGATCCCCGCGTCAGGTGACTCTAATTCCCGGAGACGGAATCGGTCCTCTGGTGACCAACGCGGTCGAGCAAGTGATGGAGGCAATGCACGCTCCCGTCTACTTCGAGCGCTACGAAGTCCATGGCGATATGAAGCAGGTTCCTCGAGAGGTGATGGAGTCTATTTTGAAGAATAAGGTGTGTTTGAAAGGCGGGCTGATTACTCCGATGGGCGGCGGTGTGAGTTCACTGAATGTGCAGCTCAGGAAGGAGCTCGACTTGTACGCCTCGCTTGTTAATTGCTTCAATTTCCCCGGATTTCCGACGCGTCATGAGAATGTCGACATCGTCGTCATTAGGGAGAATACGGAGGGCGAATACTCTGGCCTCGAGCATGAGGTCATCCCTGGCGTCGTCGAAAGCCTCAAGGTGATTACAAAGTTTTCCTCAGAACGCATTGCCAAATATGCATTTGAGTATGCTTATCTGAACAACAGAAAAAAAGTGACGGCGGTGCACAAAGCCAACATTATGAAACTTGCAGATGGTCTGTTTTTGGAGTCTTGCAGGGAGGTGGCAGCGCAATATCCTAGTATCCAATACAATGAGATTATTGTTGACAACTGTTGTATGCAGCTTGTTTCAAAACCTGAGCAATTTGATGTAATGGTGACTCCTAACCTTTATGGCAATCTAGTGGCCAACACTGCAGCTGGAATTGCTGGGGGTACTGGTCTCATGCCAGGAGGGAATGTGGGGTCAGATCATGCGATATTTGAGCAAGGAGCTTCAGCAGGGAACGTGGGGAATGAGAAAATAGTGGAGCAGAAGAAGGCAAACCCGGTGGCTTTGCTGCTGTCATCGGCCATGATGCTGAGGCACCTTCAGTTCCCTTCCTTCGCTGACAGACTCGAGAATGCTGTCAAAGCTGTCATCTTGGAGGCCAAGTGCAGGACCAAGGATCTTGGTGGAGACTGCACCACCCAAGATGTTGTCGATGCTGTCATTTCCCTTTTGGAC

Protein sequence

AARRAFPIIRHLTSPCRSRSRILPLRSDTRSVTYMPRPGEGSPRQVTLIPGDGIGPLVTNAVEQVMEAMHAPVYFERYEVHGDMKQVPREVMESILKNKVCLKGGLITPMGGGVSSLNVQLRKELDLYASLVNCFNFPGFPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVITKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAAQYPSIQYNEIIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGSDHAIFEQGASAGNVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKAVILEAKCRTKDLGGDCTTQDVVDAVISLLD
Homology
BLAST of MS005001 vs. NCBI nr
Match: XP_022140600.1 (isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like [Momordica charantia])

HSP 1 Score: 736.5 bits (1900), Expect = 1.1e-208
Identity = 372/373 (99.73%), Postives = 372/373 (99.73%), Query Frame = 0

Query: 1   AARRAFPIIRHLTSPCRSRSRILPLRSDTRSVTYMPRPGEGSPRQVTLIPGDGIGPLVTN 60
           AARRAFPIIRHLTSPCRSRSRILPLR DTRSVTYMPRPGEGSPRQVTLIPGDGIGPLVTN
Sbjct: 2   AARRAFPIIRHLTSPCRSRSRILPLRFDTRSVTYMPRPGEGSPRQVTLIPGDGIGPLVTN 61

Query: 61  AVEQVMEAMHAPVYFERYEVHGDMKQVPREVMESILKNKVCLKGGLITPMGGGVSSLNVQ 120
           AVEQVMEAMHAPVYFERYEVHGDMKQVPREVMESILKNKVCLKGGLITPMGGGVSSLNVQ
Sbjct: 62  AVEQVMEAMHAPVYFERYEVHGDMKQVPREVMESILKNKVCLKGGLITPMGGGVSSLNVQ 121

Query: 121 LRKELDLYASLVNCFNFPGFPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVITK 180
           LRKELDLYASLVNCFNFPGFPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVITK
Sbjct: 122 LRKELDLYASLVNCFNFPGFPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVITK 181

Query: 181 FSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAAQYPSIQYNEIIVD 240
           FSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAAQYPSIQYNEIIVD
Sbjct: 182 FSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAAQYPSIQYNEIIVD 241

Query: 241 NCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGSDHAIFEQGASAGN 300
           NCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGSDHAIFEQGASAGN
Sbjct: 242 NCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGSDHAIFEQGASAGN 301

Query: 301 VGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKAVILEAKCRTKDLGGDCT 360
           VGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKAVILEAKCRTKDLGGDCT
Sbjct: 302 VGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKAVILEAKCRTKDLGGDCT 361

Query: 361 TQDVVDAVISLLD 374
           TQDVVDAVISLLD
Sbjct: 362 TQDVVDAVISLLD 374

BLAST of MS005001 vs. NCBI nr
Match: XP_022971037.1 (isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 672.2 bits (1733), Expect = 2.7e-189
Identity = 340/375 (90.67%), Postives = 354/375 (94.40%), Query Frame = 0

Query: 1   AARRAFPIIRHLTSPCRSRSRILP--LRSDTRSVTYMPRPGEGSPRQVTLIPGDGIGPLV 60
           AAR AFPII  L SP RSR ++ P  L SD RSVTYMPRPG+G+PR VTLIPGDGIGPLV
Sbjct: 2   AARSAFPIICRLASPYRSRFQLSPFNLFSDARSVTYMPRPGDGAPRAVTLIPGDGIGPLV 61

Query: 61  TNAVEQVMEAMHAPVYFERYEVHGDMKQVPREVMESILKNKVCLKGGLITPMGGGVSSLN 120
           TNAVEQVMEA+HAPVYFERY+VHG+MKQVPREVMESI KNKVCLKGGL+TPMGGGVSSLN
Sbjct: 62  TNAVEQVMEALHAPVYFERYDVHGNMKQVPREVMESIFKNKVCLKGGLMTPMGGGVSSLN 121

Query: 121 VQLRKELDLYASLVNCFNFPGFPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVI 180
           VQLRKELDLYASLVNCFNFPG  TRHENVDI VIRENTEGEYSGLEHEVIPGVVESLKVI
Sbjct: 122 VQLRKELDLYASLVNCFNFPGLLTRHENVDIAVIRENTEGEYSGLEHEVIPGVVESLKVI 181

Query: 181 TKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAAQYPSIQYNEII 240
           TKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVA QYP IQYNE+I
Sbjct: 182 TKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPIIQYNEVI 241

Query: 241 VDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGSDHAIFEQGASA 300
           VDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAG+AGGTGLMPGGNVG+DHAIFEQGASA
Sbjct: 242 VDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGLAGGTGLMPGGNVGADHAIFEQGASA 301

Query: 301 GNVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKAVILEAKCRTKDLGGD 360
           GNVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVK VI+E KCRTKDLGG+
Sbjct: 302 GNVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKRVIMEDKCRTKDLGGN 361

Query: 361 CTTQDVVDAVISLLD 374
           CTTQDVVDAVI+ LD
Sbjct: 362 CTTQDVVDAVIAFLD 376

BLAST of MS005001 vs. NCBI nr
Match: XP_023534325.1 (isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 671.0 bits (1730), Expect = 5.9e-189
Identity = 339/375 (90.40%), Postives = 354/375 (94.40%), Query Frame = 0

Query: 1   AARRAFPIIRHLTSPCRSRSRILP--LRSDTRSVTYMPRPGEGSPRQVTLIPGDGIGPLV 60
           AAR AFPII  L SP RSR ++ P  L SD RSVTYMPRPG+G+PR VTLIPGDGIGPLV
Sbjct: 2   AARSAFPIICRLASPYRSRFQLSPFNLFSDARSVTYMPRPGDGAPRAVTLIPGDGIGPLV 61

Query: 61  TNAVEQVMEAMHAPVYFERYEVHGDMKQVPREVMESILKNKVCLKGGLITPMGGGVSSLN 120
           TNAVEQVMEA+HAPVYFERY+VHG+MKQVPREVMESI KNKVCLKGGL+TPMGGGVSSLN
Sbjct: 62  TNAVEQVMEALHAPVYFERYDVHGNMKQVPREVMESIFKNKVCLKGGLMTPMGGGVSSLN 121

Query: 121 VQLRKELDLYASLVNCFNFPGFPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVI 180
           VQLRKELDLYASLVNCFNFPG  TRHENVDI VIRENTEGEY+GLEHEVIPGVVESLKVI
Sbjct: 122 VQLRKELDLYASLVNCFNFPGLLTRHENVDIAVIRENTEGEYAGLEHEVIPGVVESLKVI 181

Query: 181 TKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAAQYPSIQYNEII 240
           TKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVA QYP IQYNE+I
Sbjct: 182 TKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPIIQYNEVI 241

Query: 241 VDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGSDHAIFEQGASA 300
           VDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAG+AGGTGLMPGGNVG+DHAIFEQGASA
Sbjct: 242 VDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGLAGGTGLMPGGNVGADHAIFEQGASA 301

Query: 301 GNVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKAVILEAKCRTKDLGGD 360
           GNVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVK VI+E KCRTKDLGG+
Sbjct: 302 GNVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKRVIMEDKCRTKDLGGN 361

Query: 361 CTTQDVVDAVISLLD 374
           CTTQDVVDAVI+ LD
Sbjct: 362 CTTQDVVDAVIAFLD 376

BLAST of MS005001 vs. NCBI nr
Match: KAG6604970.1 (Isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 670.6 bits (1729), Expect = 7.7e-189
Identity = 338/375 (90.13%), Postives = 354/375 (94.40%), Query Frame = 0

Query: 1   AARRAFPIIRHLTSPCRSRSRILP--LRSDTRSVTYMPRPGEGSPRQVTLIPGDGIGPLV 60
           AAR AFPII  L SP RSR ++ P  L SD RSVTYMPRPG+G+PR VTLIPGDGIGPLV
Sbjct: 2   AARSAFPIICRLASPYRSRFQLSPFNLFSDARSVTYMPRPGDGAPRAVTLIPGDGIGPLV 61

Query: 61  TNAVEQVMEAMHAPVYFERYEVHGDMKQVPREVMESILKNKVCLKGGLITPMGGGVSSLN 120
           TNAVEQVMEA+HAPVYFERY+VHG+MKQ+PREVMESI KNKVCLKGGL+TPMGGGVSSLN
Sbjct: 62  TNAVEQVMEALHAPVYFERYDVHGNMKQIPREVMESIFKNKVCLKGGLMTPMGGGVSSLN 121

Query: 121 VQLRKELDLYASLVNCFNFPGFPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVI 180
           VQLRKELDLYASLVNCFNFPG  TRHENVDI VIRENTEGEY+GLEHEVIPGVVESLKVI
Sbjct: 122 VQLRKELDLYASLVNCFNFPGLLTRHENVDIAVIRENTEGEYAGLEHEVIPGVVESLKVI 181

Query: 181 TKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAAQYPSIQYNEII 240
           TKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVA QYP IQYNE+I
Sbjct: 182 TKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPIIQYNEVI 241

Query: 241 VDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGSDHAIFEQGASA 300
           VDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAG+AGGTGLMPGGNVG+DHAIFEQGASA
Sbjct: 242 VDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGLAGGTGLMPGGNVGADHAIFEQGASA 301

Query: 301 GNVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKAVILEAKCRTKDLGGD 360
           GNVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVK VI+E KCRTKDLGG+
Sbjct: 302 GNVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKRVIMEDKCRTKDLGGN 361

Query: 361 CTTQDVVDAVISLLD 374
           CTTQDVVDAVI+ LD
Sbjct: 362 CTTQDVVDAVIAFLD 376

BLAST of MS005001 vs. NCBI nr
Match: KAG7035004.1 (Isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 670.2 bits (1728), Expect = 1.0e-188
Identity = 338/375 (90.13%), Postives = 354/375 (94.40%), Query Frame = 0

Query: 1   AARRAFPIIRHLTSPCRSRSRILP--LRSDTRSVTYMPRPGEGSPRQVTLIPGDGIGPLV 60
           AAR AFPII  L SP RSR ++ P  L SD RSVTYMPRPG+G+PR VTLIPGDGIGPLV
Sbjct: 2   AARSAFPIICRLASPYRSRFQLSPFNLFSDARSVTYMPRPGDGAPRAVTLIPGDGIGPLV 61

Query: 61  TNAVEQVMEAMHAPVYFERYEVHGDMKQVPREVMESILKNKVCLKGGLITPMGGGVSSLN 120
           TNAVEQVMEA+HAPVYFERY+VHG+MKQVPREVMESI KNKVCLKGGL+TPMGGGVSSLN
Sbjct: 62  TNAVEQVMEALHAPVYFERYDVHGNMKQVPREVMESIFKNKVCLKGGLMTPMGGGVSSLN 121

Query: 121 VQLRKELDLYASLVNCFNFPGFPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVI 180
           VQLRKELDLYASLVNCFNFPG  TRHENVDI VIRENTEGEY+GLEHEVIPGVVESLKVI
Sbjct: 122 VQLRKELDLYASLVNCFNFPGLLTRHENVDIAVIRENTEGEYAGLEHEVIPGVVESLKVI 181

Query: 181 TKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAAQYPSIQYNEII 240
           TKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVA QYP IQYNE+I
Sbjct: 182 TKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPIIQYNEVI 241

Query: 241 VDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGSDHAIFEQGASA 300
           VDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAG+AGGTGLMPGGNVG+DHAIFEQGASA
Sbjct: 242 VDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGLAGGTGLMPGGNVGADHAIFEQGASA 301

Query: 301 GNVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKAVILEAKCRTKDLGGD 360
           GNVGNEKIVEQKKANPVALL+SSAMMLRHLQFPSFADRLENAVK VI+E KCRTKDLGG+
Sbjct: 302 GNVGNEKIVEQKKANPVALLMSSAMMLRHLQFPSFADRLENAVKRVIMEDKCRTKDLGGN 361

Query: 361 CTTQDVVDAVISLLD 374
           CTTQDVVDAVI+ LD
Sbjct: 362 CTTQDVVDAVIAFLD 376

BLAST of MS005001 vs. ExPASy Swiss-Prot
Match: Q8LFC0 (Isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IDH1 PE=1 SV=2)

HSP 1 Score: 614.0 bits (1582), Expect = 1.1e-174
Identity = 304/345 (88.12%), Postives = 325/345 (94.20%), Query Frame = 0

Query: 29  TRSVTYMPRPGEGSPRQVTLIPGDGIGPLVTNAVEQVMEAMHAPVYFERYEVHGDMKQVP 88
           TRSVTYMPRPG+G+PR VTLIPGDGIGPLVTNAVEQVMEAMHAP++FE+Y+VHG+M +VP
Sbjct: 23  TRSVTYMPRPGDGAPRAVTLIPGDGIGPLVTNAVEQVMEAMHAPIFFEKYDVHGEMSRVP 82

Query: 89  REVMESILKNKVCLKGGLITPMGGGVSSLNVQLRKELDLYASLVNCFNFPGFPTRHENVD 148
            EVMESI KNKVCLKGGL TP+GGGVSSLNVQLRKELDL+ASLVNCFN PG PTRHENVD
Sbjct: 83  PEVMESIRKNKVCLKGGLKTPVGGGVSSLNVQLRKELDLFASLVNCFNLPGLPTRHENVD 142

Query: 149 IVVIRENTEGEYSGLEHEVIPGVVESLKVITKFSSERIAKYAFEYAYLNNRKKVTAVHKA 208
           IVVIRENTEGEY+GLEHEV+PGVVESLKVITKF SERIAKYAFEYAYLNNRKKVTAVHKA
Sbjct: 143 IVVIRENTEGEYAGLEHEVVPGVVESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKA 202

Query: 209 NIMKLADGLFLESCREVAAQYPSIQYNEIIVDNCCMQLVSKPEQFDVMVTPNLYGNLVAN 268
           NIMKLADGLFLESCREVA +YPSI YNEIIVDNCCMQLV+KPEQFDVMVTPNLYGNLVAN
Sbjct: 203 NIMKLADGLFLESCREVAKKYPSITYNEIIVDNCCMQLVAKPEQFDVMVTPNLYGNLVAN 262

Query: 269 TAAGIAGGTGLMPGGNVGSDHAIFEQGASAGNVGNEKIVEQKKANPVALLLSSAMMLRHL 328
           TAAGIAGGTG+MPGGNVG+DHA+FEQGASAGNVG +KIV + KANPVALLLSSAMMLRHL
Sbjct: 263 TAAGIAGGTGVMPGGNVGADHAVFEQGASAGNVGKDKIVLENKANPVALLLSSAMMLRHL 322

Query: 329 QFPSFADRLENAVKAVILEAKCRTKDLGGDCTTQDVVDAVISLLD 374
           QFPSFADRLE AVK VI E KCRTKDLGG  TTQ+VVDAVI+ LD
Sbjct: 323 QFPSFADRLETAVKKVIAEGKCRTKDLGGTSTTQEVVDAVIAKLD 367

BLAST of MS005001 vs. ExPASy Swiss-Prot
Match: O81796 (Isocitrate dehydrogenase [NAD] regulatory subunit 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IDH3 PE=1 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 2.9e-170
Identity = 302/372 (81.18%), Postives = 333/372 (89.52%), Query Frame = 0

Query: 2   ARRAFPIIRHLTSPCRSRSRILPLRSDTRSVTYMPRPGEGSPRQVTLIPGDGIGPLVTNA 61
           ARR+  I   L +   S     P  S +RS+TYMPRPG+G+PR VTLIPGDGIGPLVT A
Sbjct: 2   ARRSVSIFNRLLANPPS-----PFTSLSRSITYMPRPGDGAPRTVTLIPGDGIGPLVTGA 61

Query: 62  VEQVMEAMHAPVYFERYEVHGDMKQVPREVMESILKNKVCLKGGLITPMGGGVSSLNVQL 121
           VEQVMEAMHAPV+FERYEV G+M++VP EV+ES+ +NKVCLKGGL TP+GGGVSSLN+QL
Sbjct: 62  VEQVMEAMHAPVHFERYEVLGNMRKVPEEVIESVKRNKVCLKGGLATPVGGGVSSLNMQL 121

Query: 122 RKELDLYASLVNCFNFPGFPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVITKF 181
           RKELD++ASLVNC N PG  TRHENVDIVVIRENTEGEYSGLEHEV+PGVVESLKVITKF
Sbjct: 122 RKELDIFASLVNCINVPGLVTRHENVDIVVIRENTEGEYSGLEHEVVPGVVESLKVITKF 181

Query: 182 SSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAAQYPSIQYNEIIVDN 241
            SERIA+YAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVA  Y  I YNEIIVDN
Sbjct: 182 CSERIARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAKHYSGITYNEIIVDN 241

Query: 242 CCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGSDHAIFEQGASAGNV 301
           CCMQLV+KPEQFDVMVTPNLYGNL+ANTAAGIAGGTG+MPGGNVG++HAIFEQGASAGNV
Sbjct: 242 CCMQLVAKPEQFDVMVTPNLYGNLIANTAAGIAGGTGVMPGGNVGAEHAIFEQGASAGNV 301

Query: 302 GNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKAVILEAKCRTKDLGGDCTT 361
           GN+K+VEQKKANPVALLLSSAMMLRHL+FP+FADRLE AVK VI E K RTKDLGGDCTT
Sbjct: 302 GNDKMVEQKKANPVALLLSSAMMLRHLRFPTFADRLETAVKQVIKEGKYRTKDLGGDCTT 361

Query: 362 QDVVDAVISLLD 374
           Q+VVDAVI+ L+
Sbjct: 362 QEVVDAVIAALE 368

BLAST of MS005001 vs. ExPASy Swiss-Prot
Match: P93032 (Isocitrate dehydrogenase [NAD] regulatory subunit 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IDH2 PE=1 SV=2)

HSP 1 Score: 593.6 bits (1529), Expect = 1.6e-168
Identity = 299/372 (80.38%), Postives = 333/372 (89.52%), Query Frame = 0

Query: 2   ARRAFPIIRHLTSPCRSRSRILPLRSDTRSVTYMPRPGEGSPRQVTLIPGDGIGPLVTNA 61
           +R++F ++++L S   S S+I      TRSVTYMPRPG+G PR VTLIPGDG+GPLVTNA
Sbjct: 2   SRQSFSLLKNLRS-IASGSKI-----QTRSVTYMPRPGDGKPRPVTLIPGDGVGPLVTNA 61

Query: 62  VEQVMEAMHAPVYFERYEVHGDMKQVPREVMESILKNKVCLKGGLITPMGGGVSSLNVQL 121
           V+QVMEAMHAPVYFE +EVHGDMK +P  ++ESI KNKVCLKGGL TP+GGGVSSLNV L
Sbjct: 62  VQQVMEAMHAPVYFEPFEVHGDMKSLPEGLLESIKKNKVCLKGGLKTPVGGGVSSLNVNL 121

Query: 122 RKELDLYASLVNCFNFPGFPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVITKF 181
           RKELDL+ASLVNCFN PG  +RHENVDIVVIRENTEGEY+GLEHEV+PGVVESLKVITKF
Sbjct: 122 RKELDLFASLVNCFNLPGLASRHENVDIVVIRENTEGEYAGLEHEVVPGVVESLKVITKF 181

Query: 182 SSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAAQYPSIQYNEIIVDN 241
            SERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESC+EVA +YPSI YNEIIVDN
Sbjct: 182 CSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCQEVAKKYPSIAYNEIIVDN 241

Query: 242 CCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGSDHAIFEQGASAGNV 301
           CCMQLV++PEQFDVMVTPNLYGNLVANTAAGIAGGTG+MPGGNVG+++A+FEQGASAGNV
Sbjct: 242 CCMQLVARPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGAEYAVFEQGASAGNV 301

Query: 302 GNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKAVILEAKCRTKDLGGDCTT 361
           G +   EQK ANPVALLLSSAMMLRHLQFPSFADRLE AVK VI E  CRT+DLGG+ TT
Sbjct: 302 GKDTTEEQKNANPVALLLSSAMMLRHLQFPSFADRLETAVKRVIAEGNCRTEDLGGNSTT 361

Query: 362 QDVVDAVISLLD 374
           Q+VVDAVI+ LD
Sbjct: 362 QEVVDAVIANLD 367

BLAST of MS005001 vs. ExPASy Swiss-Prot
Match: Q54B68 (Isocitrate dehydrogenase [NAD] regulatory subunit B, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=idhB PE=3 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 2.9e-90
Identity = 183/358 (51.12%), Postives = 239/358 (66.76%), Query Frame = 0

Query: 19  RSRILPLRSDTRSV-TYMPRPGEGSPRQVTLIPGDGIGPLVTNAVEQVMEAMHAPVYFER 78
           R R +   S + S+  Y+        + VT+IPGDGIGP +T++V  V +A   P+ +E 
Sbjct: 4   RLRTVVKASSSNSIRNYLGYTSGVQKKTVTVIPGDGIGPEITSSVMGVFQAAKVPIEWEI 63

Query: 79  YEVHGDMKQVPREVMESILKNKVCLKGGLITPMGGGVSSLNVQLRKELDLYASLVNCFNF 138
           +++ G  + + +E++ SI +NKV LKG L T +  G  S N++LRK LDLYA +V C   
Sbjct: 64  FDISGG-QPISQELIASITRNKVALKGPLYTEILSGSQSRNMELRKALDLYAHVVPCKQI 123

Query: 139 PGFPTRHEN--VDIVVIRENTEGEYSGLEHEVIPGVVESLKVITKFSSERIAKYAFEYAY 198
           PG   RH++  VD VVIRENT+GEYSGLE  + PGVV+SLK+ITK +SERIA+YAFEYA 
Sbjct: 124 PGITARHDDVLVDFVVIRENTQGEYSGLEQVLTPGVVQSLKIITKEASERIARYAFEYAK 183

Query: 199 LNNRKKVTAVHKANIMKLADGLFLESCREVAAQYPSIQYNEIIVDNCCMQLVSKPEQFDV 258
            N RKKVTAVHKANI K  DGLFL +C ++A +YP I++   I+DNCCMQLV  PEQ+DV
Sbjct: 184 ANGRKKVTAVHKANIQKQTDGLFLATCTQIAKEYPEIKFENTIIDNCCMQLVKSPEQYDV 243

Query: 259 MVTPNLYGNLVANTAAGIAGGTGLMPGGNVGSDHAIFEQGASAGNVGNEKIVEQKKANPV 318
           MVTPNLYGN+V+N  A + GG GL  G NVG    IFE GA   +V  + I  + KANP 
Sbjct: 244 MVTPNLYGNIVSNIGAALVGGPGLAGGANVGEGSIIFEMGAH--HVAAD-IAGKDKANPT 303

Query: 319 ALLLSSAMMLRHLQFPSFADRLENAVKAVILEAKCRTKDLGGDCTTQDVVDAVISLLD 374
            LLL+S MML+HL     A ++ENAVKAVI E    T D+GG  +T+    AVI  ++
Sbjct: 304 GLLLASVMMLKHLGLNEHATKVENAVKAVIKEGTL-TSDIGGKSSTKQFTGAVIDYIE 356

BLAST of MS005001 vs. ExPASy Swiss-Prot
Match: O13696 (Isocitrate dehydrogenase [NAD] subunit 1, mitochondrial OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=idh1 PE=1 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 3.0e-87
Identity = 172/340 (50.59%), Postives = 230/340 (67.65%), Query Frame = 0

Query: 41  GSPRQVTLIPGDGIGPLVTNAVEQVMEAMHAPVYFERYEVHGDMK------QVPREVMES 100
           G    VTLIPGDGIG   +NAV ++ +  + P+ FE  +V G  K          E ++S
Sbjct: 18  GGKYTVTLIPGDGIGRETSNAVTEIFKTANVPIEFEEIDVTGMEKNNKSSGDALHEAIQS 77

Query: 101 ILKNKVCLKGGLITPM-GGGVSSLNVQLRKELDLYASLVNCFNFPGFPTRHENVDIVVIR 160
           + +NKV LKG L TP   GG +S NV LRKELD+YASLV   N PGF TRH+NVD  +IR
Sbjct: 78  LKRNKVGLKGILFTPFEKGGHTSFNVALRKELDIYASLVLIKNIPGFKTRHDNVDFAIIR 137

Query: 161 ENTEGEYSGLEHEVIPGVVESLKVITKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKL 220
           ENTEGEYSGLEH+ +PGVVESLK+IT++ S+RIA++AF++A  N RK VT +HKANIMKL
Sbjct: 138 ENTEGEYSGLEHQSVPGVVESLKIITEYKSKRIAQFAFDFALQNGRKSVTCIHKANIMKL 197

Query: 221 ADGLFLESCREVAAQYPSIQYNEIIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGI 280
           ADGLF  +  +VA  Y +I   ++IVDN  MQ VS+P+QFDV+V PNLYG++++N  + +
Sbjct: 198 ADGLFRRTFYDVANGYDAITPKDLIVDNASMQAVSRPQQFDVLVMPNLYGSILSNIGSAL 257

Query: 281 AGGTGLMPGGNVGSDHAIFEQGASAGNVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSF 340
            GG G++PG N G D+A+FE G    +VG   I  + +ANP A +LS+ +MLRHL    +
Sbjct: 258 VGGPGVIPGANFGRDYALFEPGCR--HVG-LSITGRGEANPTAAILSACLMLRHLGLKDY 317

Query: 341 ADRLENAVKAVILEAKCRTKDLGGDCTTQDVVDAVISLLD 374
           AD +  A  +VI E K  TKDLGG  +T D   A++  ++
Sbjct: 318 ADLINAATYSVIEEGKTLTKDLGGSASTGDFTHAILERME 354

BLAST of MS005001 vs. ExPASy TrEMBL
Match: A0A6J1CFI7 (isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like OS=Momordica charantia OX=3673 GN=LOC111011213 PE=3 SV=1)

HSP 1 Score: 736.5 bits (1900), Expect = 5.5e-209
Identity = 372/373 (99.73%), Postives = 372/373 (99.73%), Query Frame = 0

Query: 1   AARRAFPIIRHLTSPCRSRSRILPLRSDTRSVTYMPRPGEGSPRQVTLIPGDGIGPLVTN 60
           AARRAFPIIRHLTSPCRSRSRILPLR DTRSVTYMPRPGEGSPRQVTLIPGDGIGPLVTN
Sbjct: 2   AARRAFPIIRHLTSPCRSRSRILPLRFDTRSVTYMPRPGEGSPRQVTLIPGDGIGPLVTN 61

Query: 61  AVEQVMEAMHAPVYFERYEVHGDMKQVPREVMESILKNKVCLKGGLITPMGGGVSSLNVQ 120
           AVEQVMEAMHAPVYFERYEVHGDMKQVPREVMESILKNKVCLKGGLITPMGGGVSSLNVQ
Sbjct: 62  AVEQVMEAMHAPVYFERYEVHGDMKQVPREVMESILKNKVCLKGGLITPMGGGVSSLNVQ 121

Query: 121 LRKELDLYASLVNCFNFPGFPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVITK 180
           LRKELDLYASLVNCFNFPGFPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVITK
Sbjct: 122 LRKELDLYASLVNCFNFPGFPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVITK 181

Query: 181 FSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAAQYPSIQYNEIIVD 240
           FSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAAQYPSIQYNEIIVD
Sbjct: 182 FSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAAQYPSIQYNEIIVD 241

Query: 241 NCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGSDHAIFEQGASAGN 300
           NCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGSDHAIFEQGASAGN
Sbjct: 242 NCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGSDHAIFEQGASAGN 301

Query: 301 VGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKAVILEAKCRTKDLGGDCT 360
           VGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKAVILEAKCRTKDLGGDCT
Sbjct: 302 VGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKAVILEAKCRTKDLGGDCT 361

Query: 361 TQDVVDAVISLLD 374
           TQDVVDAVISLLD
Sbjct: 362 TQDVVDAVISLLD 374

BLAST of MS005001 vs. ExPASy TrEMBL
Match: A0A6J1I0V5 (isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111469830 PE=3 SV=1)

HSP 1 Score: 672.2 bits (1733), Expect = 1.3e-189
Identity = 340/375 (90.67%), Postives = 354/375 (94.40%), Query Frame = 0

Query: 1   AARRAFPIIRHLTSPCRSRSRILP--LRSDTRSVTYMPRPGEGSPRQVTLIPGDGIGPLV 60
           AAR AFPII  L SP RSR ++ P  L SD RSVTYMPRPG+G+PR VTLIPGDGIGPLV
Sbjct: 2   AARSAFPIICRLASPYRSRFQLSPFNLFSDARSVTYMPRPGDGAPRAVTLIPGDGIGPLV 61

Query: 61  TNAVEQVMEAMHAPVYFERYEVHGDMKQVPREVMESILKNKVCLKGGLITPMGGGVSSLN 120
           TNAVEQVMEA+HAPVYFERY+VHG+MKQVPREVMESI KNKVCLKGGL+TPMGGGVSSLN
Sbjct: 62  TNAVEQVMEALHAPVYFERYDVHGNMKQVPREVMESIFKNKVCLKGGLMTPMGGGVSSLN 121

Query: 121 VQLRKELDLYASLVNCFNFPGFPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVI 180
           VQLRKELDLYASLVNCFNFPG  TRHENVDI VIRENTEGEYSGLEHEVIPGVVESLKVI
Sbjct: 122 VQLRKELDLYASLVNCFNFPGLLTRHENVDIAVIRENTEGEYSGLEHEVIPGVVESLKVI 181

Query: 181 TKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAAQYPSIQYNEII 240
           TKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVA QYP IQYNE+I
Sbjct: 182 TKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPIIQYNEVI 241

Query: 241 VDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGSDHAIFEQGASA 300
           VDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAG+AGGTGLMPGGNVG+DHAIFEQGASA
Sbjct: 242 VDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGLAGGTGLMPGGNVGADHAIFEQGASA 301

Query: 301 GNVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKAVILEAKCRTKDLGGD 360
           GNVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVK VI+E KCRTKDLGG+
Sbjct: 302 GNVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKRVIMEDKCRTKDLGGN 361

Query: 361 CTTQDVVDAVISLLD 374
           CTTQDVVDAVI+ LD
Sbjct: 362 CTTQDVVDAVIAFLD 376

BLAST of MS005001 vs. ExPASy TrEMBL
Match: A0A6J1J614 (isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111483775 PE=3 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 2.4e-188
Identity = 337/375 (89.87%), Postives = 354/375 (94.40%), Query Frame = 0

Query: 1   AARRAFPIIRHLTSPCRSRSRILP--LRSDTRSVTYMPRPGEGSPRQVTLIPGDGIGPLV 60
           A RRAFPIIR L SP RSRSR+ P  L  D+RSVTYMPRPGEG  R VTLIPGDGIGPLV
Sbjct: 2   AGRRAFPIIRGLISPSRSRSRLSPFNLFFDSRSVTYMPRPGEGDLRPVTLIPGDGIGPLV 61

Query: 61  TNAVEQVMEAMHAPVYFERYEVHGDMKQVPREVMESILKNKVCLKGGLITPMGGGVSSLN 120
           TNAVEQVMEAMHAPVYFERYEVHGDMK+VPREVMESILKNKVCLKGGL+TPMGGGVSSLN
Sbjct: 62  TNAVEQVMEAMHAPVYFERYEVHGDMKEVPREVMESILKNKVCLKGGLMTPMGGGVSSLN 121

Query: 121 VQLRKELDLYASLVNCFNFPGFPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVI 180
           VQLRKELDLYASLVNCFNFPG PTRHENVDIVVIRENTEGEYSGLEHEV+PGVVESLKVI
Sbjct: 122 VQLRKELDLYASLVNCFNFPGLPTRHENVDIVVIRENTEGEYSGLEHEVLPGVVESLKVI 181

Query: 181 TKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAAQYPSIQYNEII 240
           TKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKL+DGLFLESCREVA +YPSI+Y E+I
Sbjct: 182 TKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLSDGLFLESCREVATEYPSIEYKEVI 241

Query: 241 VDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGSDHAIFEQGASA 300
           VDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVG +HAIFEQGASA
Sbjct: 242 VDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGFEHAIFEQGASA 301

Query: 301 GNVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKAVILEAKCRTKDLGGD 360
           GNVGN+KIVE+KKANPVALLLSSAMMLRHLQFPSFADRLENAVK VILE  CRTKDLGG+
Sbjct: 302 GNVGNDKIVEEKKANPVALLLSSAMMLRHLQFPSFADRLENAVKRVILEGNCRTKDLGGN 361

Query: 361 CTTQDVVDAVISLLD 374
           CTTQ +VDAVI+ ++
Sbjct: 362 CTTQRLVDAVIAYVE 376

BLAST of MS005001 vs. ExPASy TrEMBL
Match: A0A6J1G859 (isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111451756 PE=3 SV=1)

HSP 1 Score: 666.0 bits (1717), Expect = 9.2e-188
Identity = 337/375 (89.87%), Postives = 353/375 (94.13%), Query Frame = 0

Query: 1   AARRAFPIIRHLTSPCRSRSRILP--LRSDTRSVTYMPRPGEGSPRQVTLIPGDGIGPLV 60
           AAR AF II  L SP RSR ++ P  L SD RSVTYMPRPG+G+PR VTLIPGDGIGPLV
Sbjct: 2   AARSAFHIICRLASPYRSRFQLSPFNLFSDARSVTYMPRPGDGAPRAVTLIPGDGIGPLV 61

Query: 61  TNAVEQVMEAMHAPVYFERYEVHGDMKQVPREVMESILKNKVCLKGGLITPMGGGVSSLN 120
           TNAVEQVMEA+HAPVYFERY+VHG+MK+VPREVMESI KNKVCLKGGL+TPMGGGVSSLN
Sbjct: 62  TNAVEQVMEALHAPVYFERYDVHGNMKRVPREVMESIFKNKVCLKGGLMTPMGGGVSSLN 121

Query: 121 VQLRKELDLYASLVNCFNFPGFPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVI 180
           VQLRKELDLYASLVNCFNFPG  TRHENVDI VIRENTEGEY+GLEHEVIPGVVESLKVI
Sbjct: 122 VQLRKELDLYASLVNCFNFPGLLTRHENVDIAVIRENTEGEYAGLEHEVIPGVVESLKVI 181

Query: 181 TKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAAQYPSIQYNEII 240
           TKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVA QYP IQYNE+I
Sbjct: 182 TKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPIIQYNEVI 241

Query: 241 VDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGSDHAIFEQGASA 300
           VDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAG+AGGTGLMPGGNVG+DHAIFEQGASA
Sbjct: 242 VDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGLAGGTGLMPGGNVGADHAIFEQGASA 301

Query: 301 GNVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKAVILEAKCRTKDLGGD 360
           GNVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVK VI+E KCRTKDLGG+
Sbjct: 302 GNVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKRVIMEDKCRTKDLGGN 361

Query: 361 CTTQDVVDAVISLLD 374
           CTTQDVVDAVI+ LD
Sbjct: 362 CTTQDVVDAVIAFLD 376

BLAST of MS005001 vs. ExPASy TrEMBL
Match: A0A6J1FV85 (isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111448406 PE=3 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 1.6e-187
Identity = 337/375 (89.87%), Postives = 353/375 (94.13%), Query Frame = 0

Query: 1   AARRAFPIIRHLTSPCRSRSRILP--LRSDTRSVTYMPRPGEGSPRQVTLIPGDGIGPLV 60
           A RRAFPIIR L S  RSRSR+ P  L  D+RSVTYMPRPGEG  R VTLIPGDGIGPLV
Sbjct: 2   AGRRAFPIIRGLISLSRSRSRLSPFNLFFDSRSVTYMPRPGEGDLRPVTLIPGDGIGPLV 61

Query: 61  TNAVEQVMEAMHAPVYFERYEVHGDMKQVPREVMESILKNKVCLKGGLITPMGGGVSSLN 120
           TNAVEQVMEAMHAPVYFERYEVHGDMK+VPREVMESILKNKVCLKGGL+TPMGGGVSSLN
Sbjct: 62  TNAVEQVMEAMHAPVYFERYEVHGDMKEVPREVMESILKNKVCLKGGLMTPMGGGVSSLN 121

Query: 121 VQLRKELDLYASLVNCFNFPGFPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVI 180
           VQLRKELDLYASLVNCFNFPG PTRHENVDIVVIRENTEGEYSGLEHEV+PGVVESLKVI
Sbjct: 122 VQLRKELDLYASLVNCFNFPGLPTRHENVDIVVIRENTEGEYSGLEHEVLPGVVESLKVI 181

Query: 181 TKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAAQYPSIQYNEII 240
           TKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKL+DGLFLESCREVA +YPSI+Y E+I
Sbjct: 182 TKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLSDGLFLESCREVATEYPSIEYKEVI 241

Query: 241 VDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGSDHAIFEQGASA 300
           VDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVG +HAIFEQGASA
Sbjct: 242 VDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGFEHAIFEQGASA 301

Query: 301 GNVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKAVILEAKCRTKDLGGD 360
           GNVGNEKIVE+KKANPVALLLSSAMMLRHLQFPSFADRLENAVK VILE  CRTKDLGG+
Sbjct: 302 GNVGNEKIVEEKKANPVALLLSSAMMLRHLQFPSFADRLENAVKRVILEGNCRTKDLGGN 361

Query: 361 CTTQDVVDAVISLLD 374
           CTTQ +VDAVI+ ++
Sbjct: 362 CTTQRLVDAVIAYVE 376

BLAST of MS005001 vs. TAIR 10
Match: AT4G35260.1 (isocitrate dehydrogenase 1 )

HSP 1 Score: 614.0 bits (1582), Expect = 8.0e-176
Identity = 304/345 (88.12%), Postives = 325/345 (94.20%), Query Frame = 0

Query: 29  TRSVTYMPRPGEGSPRQVTLIPGDGIGPLVTNAVEQVMEAMHAPVYFERYEVHGDMKQVP 88
           TRSVTYMPRPG+G+PR VTLIPGDGIGPLVTNAVEQVMEAMHAP++FE+Y+VHG+M +VP
Sbjct: 23  TRSVTYMPRPGDGAPRAVTLIPGDGIGPLVTNAVEQVMEAMHAPIFFEKYDVHGEMSRVP 82

Query: 89  REVMESILKNKVCLKGGLITPMGGGVSSLNVQLRKELDLYASLVNCFNFPGFPTRHENVD 148
            EVMESI KNKVCLKGGL TP+GGGVSSLNVQLRKELDL+ASLVNCFN PG PTRHENVD
Sbjct: 83  PEVMESIRKNKVCLKGGLKTPVGGGVSSLNVQLRKELDLFASLVNCFNLPGLPTRHENVD 142

Query: 149 IVVIRENTEGEYSGLEHEVIPGVVESLKVITKFSSERIAKYAFEYAYLNNRKKVTAVHKA 208
           IVVIRENTEGEY+GLEHEV+PGVVESLKVITKF SERIAKYAFEYAYLNNRKKVTAVHKA
Sbjct: 143 IVVIRENTEGEYAGLEHEVVPGVVESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKA 202

Query: 209 NIMKLADGLFLESCREVAAQYPSIQYNEIIVDNCCMQLVSKPEQFDVMVTPNLYGNLVAN 268
           NIMKLADGLFLESCREVA +YPSI YNEIIVDNCCMQLV+KPEQFDVMVTPNLYGNLVAN
Sbjct: 203 NIMKLADGLFLESCREVAKKYPSITYNEIIVDNCCMQLVAKPEQFDVMVTPNLYGNLVAN 262

Query: 269 TAAGIAGGTGLMPGGNVGSDHAIFEQGASAGNVGNEKIVEQKKANPVALLLSSAMMLRHL 328
           TAAGIAGGTG+MPGGNVG+DHA+FEQGASAGNVG +KIV + KANPVALLLSSAMMLRHL
Sbjct: 263 TAAGIAGGTGVMPGGNVGADHAVFEQGASAGNVGKDKIVLENKANPVALLLSSAMMLRHL 322

Query: 329 QFPSFADRLENAVKAVILEAKCRTKDLGGDCTTQDVVDAVISLLD 374
           QFPSFADRLE AVK VI E KCRTKDLGG  TTQ+VVDAVI+ LD
Sbjct: 323 QFPSFADRLETAVKKVIAEGKCRTKDLGGTSTTQEVVDAVIAKLD 367

BLAST of MS005001 vs. TAIR 10
Match: AT4G35650.1 (isocitrate dehydrogenase III )

HSP 1 Score: 599.4 bits (1544), Expect = 2.0e-171
Identity = 302/372 (81.18%), Postives = 333/372 (89.52%), Query Frame = 0

Query: 2   ARRAFPIIRHLTSPCRSRSRILPLRSDTRSVTYMPRPGEGSPRQVTLIPGDGIGPLVTNA 61
           ARR+  I   L +   S     P  S +RS+TYMPRPG+G+PR VTLIPGDGIGPLVT A
Sbjct: 2   ARRSVSIFNRLLANPPS-----PFTSLSRSITYMPRPGDGAPRTVTLIPGDGIGPLVTGA 61

Query: 62  VEQVMEAMHAPVYFERYEVHGDMKQVPREVMESILKNKVCLKGGLITPMGGGVSSLNVQL 121
           VEQVMEAMHAPV+FERYEV G+M++VP EV+ES+ +NKVCLKGGL TP+GGGVSSLN+QL
Sbjct: 62  VEQVMEAMHAPVHFERYEVLGNMRKVPEEVIESVKRNKVCLKGGLATPVGGGVSSLNMQL 121

Query: 122 RKELDLYASLVNCFNFPGFPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVITKF 181
           RKELD++ASLVNC N PG  TRHENVDIVVIRENTEGEYSGLEHEV+PGVVESLKVITKF
Sbjct: 122 RKELDIFASLVNCINVPGLVTRHENVDIVVIRENTEGEYSGLEHEVVPGVVESLKVITKF 181

Query: 182 SSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAAQYPSIQYNEIIVDN 241
            SERIA+YAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVA  Y  I YNEIIVDN
Sbjct: 182 CSERIARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAKHYSGITYNEIIVDN 241

Query: 242 CCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGSDHAIFEQGASAGNV 301
           CCMQLV+KPEQFDVMVTPNLYGNL+ANTAAGIAGGTG+MPGGNVG++HAIFEQGASAGNV
Sbjct: 242 CCMQLVAKPEQFDVMVTPNLYGNLIANTAAGIAGGTGVMPGGNVGAEHAIFEQGASAGNV 301

Query: 302 GNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKAVILEAKCRTKDLGGDCTT 361
           GN+K+VEQKKANPVALLLSSAMMLRHL+FP+FADRLE AVK VI E K RTKDLGGDCTT
Sbjct: 302 GNDKMVEQKKANPVALLLSSAMMLRHLRFPTFADRLETAVKQVIKEGKYRTKDLGGDCTT 361

Query: 362 QDVVDAVISLLD 374
           Q+VVDAVI+ L+
Sbjct: 362 QEVVDAVIAALE 368

BLAST of MS005001 vs. TAIR 10
Match: AT2G17130.1 (isocitrate dehydrogenase subunit 2 )

HSP 1 Score: 593.6 bits (1529), Expect = 1.1e-169
Identity = 299/372 (80.38%), Postives = 333/372 (89.52%), Query Frame = 0

Query: 2   ARRAFPIIRHLTSPCRSRSRILPLRSDTRSVTYMPRPGEGSPRQVTLIPGDGIGPLVTNA 61
           +R++F ++++L S   S S+I      TRSVTYMPRPG+G PR VTLIPGDG+GPLVTNA
Sbjct: 2   SRQSFSLLKNLRS-IASGSKI-----QTRSVTYMPRPGDGKPRPVTLIPGDGVGPLVTNA 61

Query: 62  VEQVMEAMHAPVYFERYEVHGDMKQVPREVMESILKNKVCLKGGLITPMGGGVSSLNVQL 121
           V+QVMEAMHAPVYFE +EVHGDMK +P  ++ESI KNKVCLKGGL TP+GGGVSSLNV L
Sbjct: 62  VQQVMEAMHAPVYFEPFEVHGDMKSLPEGLLESIKKNKVCLKGGLKTPVGGGVSSLNVNL 121

Query: 122 RKELDLYASLVNCFNFPGFPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVITKF 181
           RKELDL+ASLVNCFN PG  +RHENVDIVVIRENTEGEY+GLEHEV+PGVVESLKVITKF
Sbjct: 122 RKELDLFASLVNCFNLPGLASRHENVDIVVIRENTEGEYAGLEHEVVPGVVESLKVITKF 181

Query: 182 SSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAAQYPSIQYNEIIVDN 241
            SERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESC+EVA +YPSI YNEIIVDN
Sbjct: 182 CSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCQEVAKKYPSIAYNEIIVDN 241

Query: 242 CCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGSDHAIFEQGASAGNV 301
           CCMQLV++PEQFDVMVTPNLYGNLVANTAAGIAGGTG+MPGGNVG+++A+FEQGASAGNV
Sbjct: 242 CCMQLVARPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGAEYAVFEQGASAGNV 301

Query: 302 GNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKAVILEAKCRTKDLGGDCTT 361
           G +   EQK ANPVALLLSSAMMLRHLQFPSFADRLE AVK VI E  CRT+DLGG+ TT
Sbjct: 302 GKDTTEEQKNANPVALLLSSAMMLRHLQFPSFADRLETAVKRVIAEGNCRTEDLGGNSTT 361

Query: 362 QDVVDAVISLLD 374
           Q+VVDAVI+ LD
Sbjct: 362 QEVVDAVIANLD 367

BLAST of MS005001 vs. TAIR 10
Match: AT2G17130.2 (isocitrate dehydrogenase subunit 2 )

HSP 1 Score: 580.9 bits (1496), Expect = 7.5e-166
Identity = 295/372 (79.30%), Postives = 329/372 (88.44%), Query Frame = 0

Query: 2   ARRAFPIIRHLTSPCRSRSRILPLRSDTRSVTYMPRPGEGSPRQVTLIPGDGIGPLVTNA 61
           +R++F ++++L S   S S+I      TRSVTYMPRPG+G PR VTLIPGDG+GPLVTNA
Sbjct: 2   SRQSFSLLKNLRS-IASGSKI-----QTRSVTYMPRPGDGKPRPVTLIPGDGVGPLVTNA 61

Query: 62  VEQVMEAMHAPVYFERYEVHGDMKQVPREVMESILKNKVCLKGGLITPMGGGVSSLNVQL 121
           V+QVMEAMHAPVYFE +EVHGDMK +P  ++ESI KNKVCLKGGL TP+GGGVSSLNV L
Sbjct: 62  VQQVMEAMHAPVYFEPFEVHGDMKSLPEGLLESIKKNKVCLKGGLKTPVGGGVSSLNVNL 121

Query: 122 RKELDLYASLVNCFNFPGFPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVITKF 181
           RKELDL+ASLVNCFN PG  +RHENVDIVVIRENTEGEY+GLEHEV+PGVVESL    KF
Sbjct: 122 RKELDLFASLVNCFNLPGLASRHENVDIVVIRENTEGEYAGLEHEVVPGVVESL----KF 181

Query: 182 SSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAAQYPSIQYNEIIVDN 241
            SERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESC+EVA +YPSI YNEIIVDN
Sbjct: 182 CSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCQEVAKKYPSIAYNEIIVDN 241

Query: 242 CCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGSDHAIFEQGASAGNV 301
           CCMQLV++PEQFDVMVTPNLYGNLVANTAAGIAGGTG+MPGGNVG+++A+FEQGASAGNV
Sbjct: 242 CCMQLVARPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGAEYAVFEQGASAGNV 301

Query: 302 GNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKAVILEAKCRTKDLGGDCTT 361
           G +   EQK ANPVALLLSSAMMLRHLQFPSFADRLE AVK VI E  CRT+DLGG+ TT
Sbjct: 302 GKDTTEEQKNANPVALLLSSAMMLRHLQFPSFADRLETAVKRVIAEGNCRTEDLGGNSTT 361

Query: 362 QDVVDAVISLLD 374
           Q+VVDAVI+ LD
Sbjct: 362 QEVVDAVIANLD 363

BLAST of MS005001 vs. TAIR 10
Match: AT5G03290.1 (isocitrate dehydrogenase V )

HSP 1 Score: 318.2 bits (814), Expect = 9.1e-87
Identity = 163/334 (48.80%), Postives = 226/334 (67.66%), Query Frame = 0

Query: 42  SPRQVTLIPGDGIGPLVTNAVEQVMEAMHAPVYFERYEVHGDMKQVPR-------EVMES 101
           +P   TL PGDGIGP +  +V++V      P+ +E + V  ++   PR       E +ES
Sbjct: 42  TPITATLFPGDGIGPEIAESVKKVFTTAGVPIEWEEHYVGTEID--PRTQSFLTWESLES 101

Query: 102 ILKNKVCLKGGLITPMGGGVSSLNVQLRKELDLYASLVNCFNFPGFPTRHENVDIVVIRE 161
           + +NKV LKG + TP+G G  SLN+ LRKEL+LYA++  C++ PG+ TR+++VD++ IRE
Sbjct: 102 VRRNKVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVDLITIRE 161

Query: 162 NTEGEYSGLEHEVIPGVVESLKVITKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLA 221
           NTEGEYSGLEH+V+ GVVESLK+IT+ +S R+A+YAF YA  + R++V+A+HKANIM+  
Sbjct: 162 NTEGEYSGLEHQVVRGVVESLKIITRQASLRVAEYAFLYAKTHGRERVSAIHKANIMQKT 221

Query: 222 DGLFLESCREVAAQYPSIQYNEIIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIA 281
           DGLFL+ CREVA +YP I Y E+++DNCCM LV  P  FDV+V PNLYG+++++  AG+ 
Sbjct: 222 DGLFLKCCREVAEKYPEITYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLV 281

Query: 282 GGTGLMPGGNVGSDHAIFEQGASAGNVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFA 341
           GG GL P  N+G D       A A +     I  +  ANP ALLLS  MMLRHL+F   A
Sbjct: 282 GGLGLTPSCNIGEDGVAL---AEAVHGSAPDIAGKNLANPTALLLSGVMMLRHLKFNEQA 341

Query: 342 DRLENAVKAVILEAKCRTKDLGGDCTTQDVVDAV 369
           +++ +A+   I E K RT DLGG  TT +   A+
Sbjct: 342 EQIHSAIINTIAEGKYRTADLGGSSTTTEFTKAI 370

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140600.11.1e-20899.73isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like [Momordi... [more]
XP_022971037.12.7e-18990.67isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like [Cucurbi... [more]
XP_023534325.15.9e-18990.40isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like [Cucurbi... [more]
KAG6604970.17.7e-18990.13Isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial, partial [Cuc... [more]
KAG7035004.11.0e-18890.13Isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial, partial [Cuc... [more]
Match NameE-valueIdentityDescription
Q8LFC01.1e-17488.12Isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial OS=Arabidopsi... [more]
O817962.9e-17081.18Isocitrate dehydrogenase [NAD] regulatory subunit 3, mitochondrial OS=Arabidopsi... [more]
P930321.6e-16880.38Isocitrate dehydrogenase [NAD] regulatory subunit 2, mitochondrial OS=Arabidopsi... [more]
Q54B682.9e-9051.12Isocitrate dehydrogenase [NAD] regulatory subunit B, mitochondrial OS=Dictyostel... [more]
O136963.0e-8750.59Isocitrate dehydrogenase [NAD] subunit 1, mitochondrial OS=Schizosaccharomyces p... [more]
Match NameE-valueIdentityDescription
A0A6J1CFI75.5e-20999.73isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like OS=Momor... [more]
A0A6J1I0V51.3e-18990.67isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like OS=Cucur... [more]
A0A6J1J6142.4e-18889.87isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like OS=Cucur... [more]
A0A6J1G8599.2e-18889.87isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like OS=Cucur... [more]
A0A6J1FV851.6e-18789.87isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like OS=Cucur... [more]
Match NameE-valueIdentityDescription
AT4G35260.18.0e-17688.12isocitrate dehydrogenase 1 [more]
AT4G35650.12.0e-17181.18isocitrate dehydrogenase III [more]
AT2G17130.11.1e-16980.38isocitrate dehydrogenase subunit 2 [more]
AT2G17130.27.5e-16679.30isocitrate dehydrogenase subunit 2 [more]
AT5G03290.19.1e-8748.80isocitrate dehydrogenase V [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR024084Isopropylmalate dehydrogenase-like domainSMARTSM01329Iso_dh_2coord: 45..368
e-value: 1.4E-140
score: 483.0
IPR024084Isopropylmalate dehydrogenase-like domainPFAMPF00180Iso_dhcoord: 46..368
e-value: 2.3E-81
score: 273.6
NoneNo IPR availableGENE3D3.40.718.10Isopropylmalate Dehydrogenasecoord: 30..371
e-value: 1.8E-126
score: 423.7
NoneNo IPR availablePANTHERPTHR11835:SF62ISOCITRATE DEHYDROGENASE [NAD] REGULATORY SUBUNIT 1, MITOCHONDRIAL-LIKEcoord: 27..371
NoneNo IPR availablePANTHERPTHR11835DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATEcoord: 27..371
NoneNo IPR availableSUPERFAMILY53659Isocitrate/Isopropylmalate dehydrogenase-likecoord: 44..372
IPR004434Isocitrate dehydrogenase NAD-dependentTIGRFAMTIGR00175TIGR00175coord: 43..370
e-value: 6.9E-156
score: 516.5

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS005001.1MS005001.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006099 tricarboxylic acid cycle
molecular_function GO:0004449 isocitrate dehydrogenase (NAD+) activity
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor