MS004970 (gene) Bitter gourd (TR) v1

Overview
NameMS004970
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionVacuolar cation/proton exchanger
Locationscaffold176: 1777594 .. 1782594 (+)
RNA-Seq ExpressionMS004970
SyntenyMS004970
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCGGCTCAGGAGTAACGACAACAAACAAAGCGTTGAAAGGCATTCCCATGTCGAGGTCTCTGTTTCTGTTCCCTTGGTTTTTCCTATTTATTTATCTACTTAAATCAGCCTTCTCGCTCGCTGTTTGTACCCTTAAATGTTGTTTTCAACCTACGCTTTTTAGTGATTTTTTAATTTAAATAACCCTATTGGTGGTGTAACAGATGGGATCATTAGGCGAGAAAACCATACTGGAATTTGAGGATGAGAGCCTCGTTAGTCCTGTATCATTAAATAGAAAGAATTTTGCTTTTGAGGCATCTATGCACGGTTCTCCATCATCTGGTGATTCGCAGAAATTTTGTTATGGTAACATGAAGAACAGAATTATTGACAGCATTAAGATTGTTGTCTTCTCTGCTAAAATCAATCTTCTCATGCCTTTCGGCCCTTTAGCAATATTAGTCAGCAGTCTTTCTGGTCACCATGTGAGTTATCTTCCCACATGTTCTCATCAATTCATTTTGGGTTGTTATCTTGTCCCATTATGGATCCATTGCTTATGTATGTGCATTTCAGGGGTGGGTCTTCCTTTTAAGCTTACTGGGGATCATACCTCTTGCTGAGCGTCTGGGTTATGCAACGGAGTAAGGTTTCTTCAATTTTAGATTACTTGTTGTTGTAGTGAATCTGTAGAACTTTTTCCTATTAAACTTTGATTCTGTGCTTTGGATAAATCTATAGCTTTTTGGACTCTTGGATTCCATTTTATATCCTGGAAAGAGTGCAGTACAATATTTGAGCATTCTTTTAGTTTTTGACACTTCTTAAACTACCCATTTTTCCATGCAATATTGGATTTGATGCACGAAAACATGAGCCATGTATAAGTTTTAAGGTCATGTCTCGCCTTGCACCTCCACCTCATGCCTCTGCCTCAAGGAGTTTCAGTGCTTTAAAAAATCTTGTTCAGTACAATTTGGGATTTGGGGGTGCATCCCTTCAAACAAATGTCTAATAGATTTTAATAAACAGGTCATAGGTCAAAAAGGTTTGGGTCCATTAATTTGATATGCAAAGTTTCTCAAAGAATCAACTATATAACCATTAATCCCCCCATTTCCTTACCAAGTAATATAGAAAGGCCAGATCATTTTTTGGCCAGGTGAATAAAACAAACCTGTGTAATGTTTGCCTATAAATTTATTAACGTCTTATGCAATCTGATCTTCTTTTGATGCAGACAACTGGCATGCTATACTGGAGCCACAGGTAATACGATTTGAAGTTGGTTTTTTAATTCATAAATTGAATAGGAATCTTCTTAAGCTCGTAGAGATAAACACTAACCTTGATTAAACTAGTTGGTGGCTTATATTACTAACGGAGTACAATGTGGAATGATAAATTAATAGCTTCTTAATTCGAGACAATTTTGGCCAAGAAGACTGTTTTGCTGTTACGCACCCACTTAAAATTGTCACAGGAAGTAGAAACCACTAAATTACAAAGCCCCCACCCGCTTCCCCTGTCCCCCCCACCACTCTAGCCAAAGAAAGAAAGAAATGAAATAGAGTAAGCTTTTCTCCCATAATGTTTTATTTACTTCTCAAGTTTAACATAAGTACCTTCATTTTTAACTTTGCAGTTGGAGGTCTTCTGAATGCTACTTTTGGAAATGCCACAGAATTGATAATATCAATATATGCATTGCGAAGTGGAATGATACGTGTCGTTCAACAGTCATTATTAGGTTCAATTTTGTCAAATATGTTATTAGTACTTGGATGTGCCTTCTTTGCTGGTGGAGTTGTGGTTTCTAAGAGGGAGCAGGTCTTCAGTAAGGTTTGTTTTTACCTTGATAGGAGTCCTTTTGGATACTGTTTACTTATTGATCTCTGTTCTATTTCTCAAGCATACATCATGCGAATGATGCTTTCTTATTTTTAGGGATCTGCTACAGTGAATTCTGGATTGCTGTTGATGGCAGTAATGGGACTTCTATTTCCTGCTGTCCTGCATTCCACACATACAGAGTTGCACTTTGGAAAGTCAGAGTTGGCTCTTTCAAGATTTAGTAGCGGCATTATGTTGGTGGCATATGCTGCCTATCTTGTTTTTCAGTTGAAAAGCGAAAAGAACTTATATCACCCGGTTGATGTGGTCGATGAGGTTGATAGCTCTGCTTTCATACATGACCTAATGAGCATAAAATATCTTACATTCTAGTAACTAGTTTTTTTTAATTCAAATGCCATACCATGGTTGTTATAACACCTGTACCCTCAGTGGCATGATCAGTGTGCAATAGTCTTTGGATAGAGAAGTCTAATAACTGTACAGTGGCAGCATTTCACTAAAGGTTCATTTTGCTACATTGTAGATCTAGGTGGTCTTTTTTGTTCTTGATTTTGCTAGATATTATAGTTTTTAAGTATCTGATAGGAATCGGGAATCTTTTTAATTAATTGGCTAATTTTTTGAAGTGTTTCCAGAGCCTTTATTATCTCTTGGTTCTTCCTCAGTTAGAAATCTCCATTAAATTTCTAGTGTCTCACCATTGTACTGTTATATTCTTCTCCTCTTTCTTTTCTCTTGCTCCCTAACTATCTAAGATAGAATCAGCCAACCAGGAATGATGCCAACACAAACCTTGACCCCTTAACTATCATATTTCCTCTTTTGAGGAGCAAACTCGACTACTTTTCTTCTCCTGCTAGCTTCTTACGCCTCCTCATGTGCGTTAACATGGGAGGAAGTTTATTTATATCTTTCTTCTGCAGTTTATACAAGAAGTTTCTGTATCTTTACTTGACTTGATATCTGTTACGTGGAAAAATATTGATGCTGAGAAACACATATGAAACTTCTGTAGACATTTTTCAGAAACAACTTTTACCATGATATGAATAAATATATTTTAGAAGCTTTTTTTTTTGAGAAGTTACCATGAATAAATATTTACTACCAGGTATTGTTTCTAGTCGGATGTATAATATAATGCTTACATGATTGAGTTTAACTGTTTTTACGTGTAGTATGTATTCAGATTGGGAATCCTAATAACTCTACTTTTGGTCACTGGCAATATTCGGTCTCATTAGAGTTTTGTCCTTTTTTTTTTTGGAAAAAAATTTATGTTTGGTGGTTTAATATGAATAATTGGCATATCATAGGTGGAGACTGAAGAGAATTCTGATGATGAAGAATCTCCTGAGATATCTATGTGGGAATCCATTATTTGGCTGTCTATCTTGACCATTTGGATATCCGTCCTTTCTGAATATTTAGTAAATGCAATAGAGGTATTGTTGACTTGTTAATACATCCTAATATGAATCTTTTCCTTTTTTTTCCTTTTTTTTTCCGTGTTGTTGCCTATTTCTCCCTTTTCTCCTCTGCGAAGATAATTCTCACATTATTGCATATTATCTGTACAATTATATGGTTTATTTGATTACAATCTCAGGGGGCATCGGTTGCCATGGACATACCAGTAGCATTCATTAGTGTAATCCTACTACCAATTGTTGGGAATGCTGCAGAGCATGCGGGTGCCATCATGTTTGCCATGAAAGACAAGCTTGTAAGTGCTTGCCCTTTTATGTGCCTTACAAAATGATCTCTGAGGTCCTTTTCTCTAATCATGTCTTGTGTAGGACATTTCTTTGGGAGTCGCAATAGGATCGTCTACGCAGATATCTATGTTCGGGGTGAGTTCTACACAACATTTTTTATTTTATTTTGCTGAATGAAATACTTTGGACCGAGCGCAGTGTAGTTGGATATTTTTTCACTACCTTGCAGTTTGTTACCCTTGTTATTTTTTTCGTATTCATCAGCCTAAATGGCACAATATTCTTTGTGTTGCCCCTATACTTCAGTACTAGAGAGTGAAGGACTGCACCCATCCATTGGTTAAGTCTAATGATGGAGGTCATTTTGCTTTCAAGTTGCCAATTGTCAAACAATCAGAAGCAAACCCTTTTGCTCCCCAAATGCAGTTTTGATTTTCAAACTTTGCTTCAAGAGAACTGATATGGATATGGCACATAGGTCAAAACAAAAGGTTTGATGTTTATAGGTCATGAGTTGAAAGTCTCAGAGATCACCTTCTAAATTATCAAAATTTCTTATTCTACGTGGTATGTAATCAAGTTCTGCCCCTTGAAAATAGTTGAGCTGCATACAAGCTACATTTGAAACGACATTTTGTTTTTTATTTTTAAAAAGGTTCAGCTTTGCAACCCCAACTATTGAAGTACCGGCCGACCATCTTTTGTCTGTTGAACAGTGTTTTTTCCCCTCTTTTAAATGCCAAATCTGCCGTAAACGGCATAACGAGCATCATCATCACATATTAAACCATCATAGAATTGATCTTGCTCTTTCAAACAAACATATGACTGCTGATTCCACGTTATCTTTTCTTTGTTTTCTCCTTCCATAACCTGTTCATAAATTTTTATGACTGCAGATTCCTTTTTGTGTGGTCATTGGTTGGATGATGGGAGTCCCTATGGACTTGAATTTTCAACTCTTCGAGACCGCCACGCTTTTCATCACTGTTATAGTTGTCGCCTTCATGTTGCAGGTTTGCCACTTCAGGCTTCTTTTATTTTATTTAATGTGTTTACATGATAAAAACGCTTTAGAAATCAGTAAACACCCTGATGTAGTTAGGATCCTGGAATAGTTCGAAATGCAAATTGTTAAAATCACATATCCAAATTGCCAACCCCACCCCCCCATTTTACACACTGGCCCTTGTGTGGGTTTCCTCATATAAATGGTTCAATGATCACCATGATCCATAATACTTTTGAATAAATAACTGCAACCTGTAGGAATGAGGATGTGCATGATATATAATGATGATTCGAATCATTTCACATTGTGTTTGCAGGATGGAACTTCTAATTACCTCAAAGGACTGATGCTTATTCTCTGCTACCTGATAGTAGCTGCAAGTTTCTTTGTACATATTGATCCAGCTTCAGTTGGTAAGCCATCCCTC

mRNA sequence

ATGGATCGGCTCAGGAGTAACGACAACAAACAAAGCGTTGAAAGGCATTCCCATGTCGAGATGGGATCATTAGGCGAGAAAACCATACTGGAATTTGAGGATGAGAGCCTCGTTAGTCCTGTATCATTAAATAGAAAGAATTTTGCTTTTGAGGCATCTATGCACGGTTCTCCATCATCTGGTGATTCGCAGAAATTTTGTTATGGTAACATGAAGAACAGAATTATTGACAGCATTAAGATTGTTGTCTTCTCTGCTAAAATCAATCTTCTCATGCCTTTCGGCCCTTTAGCAATATTAGTCAGCAGTCTTTCTGGTCACCATGGGTGGGTCTTCCTTTTAAGCTTACTGGGGATCATACCTCTTGCTGAGCGTCTGGGTTATGCAACGGAACAACTGGCATGCTATACTGGAGCCACAGTTGGAGGTCTTCTGAATGCTACTTTTGGAAATGCCACAGAATTGATAATATCAATATATGCATTGCGAAGTGGAATGATACGTGTCGTTCAACAGTCATTATTAGGTTCAATTTTGTCAAATATGTTATTAGTACTTGGATGTGCCTTCTTTGCTGGTGGAGTTGTGGTTTCTAAGAGGGAGCAGGTCTTCAGTAAGGGATCTGCTACAGTGAATTCTGGATTGCTGTTGATGGCAGTAATGGGACTTCTATTTCCTGCTGTCCTGCATTCCACACATACAGAGTTGCACTTTGGAAAGTCAGAGTTGGCTCTTTCAAGATTTAGTAGCGGCATTATGTTGGTGGCATATGCTGCCTATCTTGTTTTTCAGTTGAAAAGCGAAAAGAACTTATATCACCCGGTTGATGTGGTGGAGACTGAAGAGAATTCTGATGATGAAGAATCTCCTGAGATATCTATGTGGGAATCCATTATTTGGCTGTCTATCTTGACCATTTGGATATCCGTCCTTTCTGAATATTTAGTAAATGCAATAGAGGGGGCATCGGTTGCCATGGACATACCAGTAGCATTCATTAGTGTAATCCTACTACCAATTGTTGGGAATGCTGCAGAGCATGCGGGTGCCATCATGTTTGCCATGAAAGACAAGCTTGACATTTCTTTGGGAGTCGCAATAGGATCGTCTACGCAGATATCTATGTTCGGGATTCCTTTTTGTGTGGTCATTGGTTGGATGATGGGAGTCCCTATGGACTTGAATTTTCAACTCTTCGAGACCGCCACGCTTTTCATCACTGTTATAGTTGTCGCCTTCATGTTGCAGGATGGAACTTCTAATTACCTCAAAGGACTGATGCTTATTCTCTGCTACCTGATAGTAGCTGCAAGTTTCTTTGTACATATTGATCCAGCTTCAGTTGGTAAGCCATCCCTC

Coding sequence (CDS)

ATGGATCGGCTCAGGAGTAACGACAACAAACAAAGCGTTGAAAGGCATTCCCATGTCGAGATGGGATCATTAGGCGAGAAAACCATACTGGAATTTGAGGATGAGAGCCTCGTTAGTCCTGTATCATTAAATAGAAAGAATTTTGCTTTTGAGGCATCTATGCACGGTTCTCCATCATCTGGTGATTCGCAGAAATTTTGTTATGGTAACATGAAGAACAGAATTATTGACAGCATTAAGATTGTTGTCTTCTCTGCTAAAATCAATCTTCTCATGCCTTTCGGCCCTTTAGCAATATTAGTCAGCAGTCTTTCTGGTCACCATGGGTGGGTCTTCCTTTTAAGCTTACTGGGGATCATACCTCTTGCTGAGCGTCTGGGTTATGCAACGGAACAACTGGCATGCTATACTGGAGCCACAGTTGGAGGTCTTCTGAATGCTACTTTTGGAAATGCCACAGAATTGATAATATCAATATATGCATTGCGAAGTGGAATGATACGTGTCGTTCAACAGTCATTATTAGGTTCAATTTTGTCAAATATGTTATTAGTACTTGGATGTGCCTTCTTTGCTGGTGGAGTTGTGGTTTCTAAGAGGGAGCAGGTCTTCAGTAAGGGATCTGCTACAGTGAATTCTGGATTGCTGTTGATGGCAGTAATGGGACTTCTATTTCCTGCTGTCCTGCATTCCACACATACAGAGTTGCACTTTGGAAAGTCAGAGTTGGCTCTTTCAAGATTTAGTAGCGGCATTATGTTGGTGGCATATGCTGCCTATCTTGTTTTTCAGTTGAAAAGCGAAAAGAACTTATATCACCCGGTTGATGTGGTGGAGACTGAAGAGAATTCTGATGATGAAGAATCTCCTGAGATATCTATGTGGGAATCCATTATTTGGCTGTCTATCTTGACCATTTGGATATCCGTCCTTTCTGAATATTTAGTAAATGCAATAGAGGGGGCATCGGTTGCCATGGACATACCAGTAGCATTCATTAGTGTAATCCTACTACCAATTGTTGGGAATGCTGCAGAGCATGCGGGTGCCATCATGTTTGCCATGAAAGACAAGCTTGACATTTCTTTGGGAGTCGCAATAGGATCGTCTACGCAGATATCTATGTTCGGGATTCCTTTTTGTGTGGTCATTGGTTGGATGATGGGAGTCCCTATGGACTTGAATTTTCAACTCTTCGAGACCGCCACGCTTTTCATCACTGTTATAGTTGTCGCCTTCATGTTGCAGGATGGAACTTCTAATTACCTCAAAGGACTGATGCTTATTCTCTGCTACCTGATAGTAGCTGCAAGTTTCTTTGTACATATTGATCCAGCTTCAGTTGGTAAGCCATCCCTC

Protein sequence

MDRLRSNDNKQSVERHSHVEMGSLGEKTILEFEDESLVSPVSLNRKNFAFEASMHGSPSSGDSQKFCYGNMKNRIIDSIKIVVFSAKINLLMPFGPLAILVSSLSGHHGWVFLLSLLGIIPLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALRSGMIRVVQQSLLGSILSNMLLVLGCAFFAGGVVVSKREQVFSKGSATVNSGLLLMAVMGLLFPAVLHSTHTELHFGKSELALSRFSSGIMLVAYAAYLVFQLKSEKNLYHPVDVVETEENSDDEESPEISMWESIIWLSILTIWISVLSEYLVNAIEGASVAMDIPVAFISVILLPIVGNAAEHAGAIMFAMKDKLDISLGVAIGSSTQISMFGIPFCVVIGWMMGVPMDLNFQLFETATLFITVIVVAFMLQDGTSNYLKGLMLILCYLIVAASFFVHIDPASVGKPSL
Homology
BLAST of MS004970 vs. NCBI nr
Match: XP_022140503.1 (vacuolar cation/proton exchanger 3-like [Momordica charantia] >XP_022140504.1 vacuolar cation/proton exchanger 3-like [Momordica charantia] >XP_022140505.1 vacuolar cation/proton exchanger 3-like [Momordica charantia])

HSP 1 Score: 837.4 bits (2162), Expect = 5.8e-239
Identity = 447/451 (99.11%), Postives = 447/451 (99.11%), Query Frame = 0

Query: 1   MDRLRSNDNKQSVERHSHVEMGSLGEKTILEFEDESLVSPVSLNRKNFAFEASMHGSPSS 60
           MDRL SNDNKQSVERHSHVEMGSLGEKTILEFEDESLVSPVSLNRKNFAFEASMHGSPSS
Sbjct: 1   MDRLGSNDNKQSVERHSHVEMGSLGEKTILEFEDESLVSPVSLNRKNFAFEASMHGSPSS 60

Query: 61  GDSQKFCYGNMKNRIIDSIKIVVFSAKINLLMPFGPLAILVSSLSGHHGWVFLLSLLGII 120
           GDSQKFCYGNMKNRIIDSIKIVVFSAKINLLMPFGPLAILVSSLSGHHGWVFLLSLLGII
Sbjct: 61  GDSQKFCYGNMKNRIIDSIKIVVFSAKINLLMPFGPLAILVSSLSGHHGWVFLLSLLGII 120

Query: 121 PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALRSGMIRVVQQSLLGSILS 180
           PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALRSGMIRVVQQSLLGSILS
Sbjct: 121 PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALRSGMIRVVQQSLLGSILS 180

Query: 181 NMLLVLGCAFFAGGVVVSKREQVFSKGSATVNSGLLLMAVMGLLFPAVLHSTHTELHFGK 240
           NMLLVLGCAFFAGGVVVSKREQVFSKGSATVNSGLLLMAVMGLLFPAVLHSTHTELHFGK
Sbjct: 181 NMLLVLGCAFFAGGVVVSKREQVFSKGSATVNSGLLLMAVMGLLFPAVLHSTHTELHFGK 240

Query: 241 SELALSRFSSGIMLVAYAAYLVFQLKSEKNLYHPVDV---VETEENSDDEESPEISMWES 300
           SELALSRFSSGIMLVAYAAYLVFQLKSEKNLYHPVDV   VETEENSDDEESPEISMWES
Sbjct: 241 SELALSRFSSGIMLVAYAAYLVFQLKSEKNLYHPVDVVDEVETEENSDDEESPEISMWES 300

Query: 301 IIWLSILTIWISVLSEYLVNAIEGASVAMDIPVAFISVILLPIVGNAAEHAGAIMFAMKD 360
           IIWLSILTIWISVLSEYLVNAIEGASVAMDIPVAFISVILLPIVGNAAEHAGAIMFAMKD
Sbjct: 301 IIWLSILTIWISVLSEYLVNAIEGASVAMDIPVAFISVILLPIVGNAAEHAGAIMFAMKD 360

Query: 361 KLDISLGVAIGSSTQISMFGIPFCVVIGWMMGVPMDLNFQLFETATLFITVIVVAFMLQD 420
           KLDISLGVAIGSSTQISMFGIPFCVVIGWMMGVPMDLNFQLFETATLFITVIVVAFMLQD
Sbjct: 361 KLDISLGVAIGSSTQISMFGIPFCVVIGWMMGVPMDLNFQLFETATLFITVIVVAFMLQD 420

Query: 421 GTSNYLKGLMLILCYLIVAASFFVHIDPASV 449
           GTSNYLKGLMLILCYLIVAASFFVHIDPASV
Sbjct: 421 GTSNYLKGLMLILCYLIVAASFFVHIDPASV 451

BLAST of MS004970 vs. NCBI nr
Match: XP_008448138.1 (PREDICTED: vacuolar cation/proton exchanger 3-like isoform X1 [Cucumis melo])

HSP 1 Score: 773.5 bits (1996), Expect = 1.0e-219
Identity = 410/449 (91.31%), Postives = 424/449 (94.43%), Query Frame = 0

Query: 1   MDRLRSNDNKQSVERHSHVEMGSLGEKTILEFEDESLVSPVSLNRKNFAFEASMHGSPSS 60
           MD  RSNDNKQ VERHS  EMGSLGEKTILEFEDESLVSPVSLNRKNF+ E S+H SP  
Sbjct: 1   MDGFRSNDNKQGVERHSRTEMGSLGEKTILEFEDESLVSPVSLNRKNFSIEGSIHSSPLG 60

Query: 61  GDSQKFCYGNMKNRIIDSIKIVVFSAKINLLMPFGPLAILVSSLSGHHGWVFLLSLLGII 120
           G SQK C  NMKNR+  SIKIVVFSAKINLLMPFGPLAI+VS+LSGHHGWVFLLSLLGII
Sbjct: 61  GGSQKLCCSNMKNRVFGSIKIVVFSAKINLLMPFGPLAIIVSTLSGHHGWVFLLSLLGII 120

Query: 121 PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALRSGMIRVVQQSLLGSILS 180
           PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALR GMIRVVQQSLLGSILS
Sbjct: 121 PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALRRGMIRVVQQSLLGSILS 180

Query: 181 NMLLVLGCAFFAGGVVVSKREQVFSKGSATVNSGLLLMAVMGLLFPAVLHSTHTELHFGK 240
           NMLLVLGCAFFAGG+VVSKREQVFSK +ATVNSGLLLMAVMGLLFPAVL STHTELH GK
Sbjct: 181 NMLLVLGCAFFAGGIVVSKREQVFSKAAATVNSGLLLMAVMGLLFPAVLRSTHTELHSGK 240

Query: 241 SELALSRFSSGIMLVAYAAYLVFQLKSEKNLYHPVDVVETEENSDDEESPEISMWESIIW 300
           SELALSRFSSGIMLVAYAAYLVFQLKS+KNLY PVD V+ E +SDDEE+PEISMWESI+W
Sbjct: 241 SELALSRFSSGIMLVAYAAYLVFQLKSDKNLYLPVDEVDNEGSSDDEEAPEISMWESIVW 300

Query: 301 LSILTIWISVLSEYLVNAIEGASVAMDIPVAFISVILLPIVGNAAEHAGAIMFAMKDKLD 360
           LSILTIWISVLSEYLVNAIEGASVAM+IPVAFISVILLPIVGNAAEHAGAIMFAMKDKLD
Sbjct: 301 LSILTIWISVLSEYLVNAIEGASVAMNIPVAFISVILLPIVGNAAEHAGAIMFAMKDKLD 360

Query: 361 ISLGVAIGSSTQISMFGIPFCVVIGWMMGVPMDLNFQLFETATLFITVIVVAFMLQDGTS 420
           ISLGVAIGSSTQISMFGIPFCVVIGW+MG PMDLNFQLFETATLFITVIVVAFMLQDGTS
Sbjct: 361 ISLGVAIGSSTQISMFGIPFCVVIGWIMGCPMDLNFQLFETATLFITVIVVAFMLQDGTS 420

Query: 421 NYLKGLMLILCYLIVAASFFVHIDPASVG 450
           NYLKGLMLILCYLIVAASFFVHIDPASVG
Sbjct: 421 NYLKGLMLILCYLIVAASFFVHIDPASVG 449

BLAST of MS004970 vs. NCBI nr
Match: KGN46624.1 (hypothetical protein Csa_005203 [Cucumis sativus])

HSP 1 Score: 764.2 bits (1972), Expect = 6.2e-217
Identity = 407/449 (90.65%), Postives = 421/449 (93.76%), Query Frame = 0

Query: 1   MDRLRSNDNKQSVERHSHVEMGSLGEKTILEFEDESLVSPVSLNRKNFAFEASMHGSPSS 60
           MD  RSNDNKQ VERHS  EMGSLGEKTILEFEDESLVSPVSL RKNF+ E S+H S   
Sbjct: 1   MDGFRSNDNKQGVERHSRTEMGSLGEKTILEFEDESLVSPVSLTRKNFSIEGSIHSSSLG 60

Query: 61  GDSQKFCYGNMKNRIIDSIKIVVFSAKINLLMPFGPLAILVSSLSGHHGWVFLLSLLGII 120
           G SQK    NMKNR+  SIKIVVFSAKINLLMPFGPLAI+VS+LSGHHGWVFLLSLLGII
Sbjct: 61  GGSQKLSCSNMKNRVFGSIKIVVFSAKINLLMPFGPLAIIVSTLSGHHGWVFLLSLLGII 120

Query: 121 PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALRSGMIRVVQQSLLGSILS 180
           PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALR GMIRVVQQSLLGSILS
Sbjct: 121 PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALRRGMIRVVQQSLLGSILS 180

Query: 181 NMLLVLGCAFFAGGVVVSKREQVFSKGSATVNSGLLLMAVMGLLFPAVLHSTHTELHFGK 240
           NMLLVLGCAFFAGG+VVSKREQVFSK +ATVNSGLLLMAVMGLLFPAVL STHTELH GK
Sbjct: 181 NMLLVLGCAFFAGGIVVSKREQVFSKAAATVNSGLLLMAVMGLLFPAVLRSTHTELHSGK 240

Query: 241 SELALSRFSSGIMLVAYAAYLVFQLKSEKNLYHPVDVVETEENSDDEESPEISMWESIIW 300
           SELALSRFSSGIMLVAYAAYLVFQLKS+KNLY PVD V+ E +SDDEE+PEISMWESI+W
Sbjct: 241 SELALSRFSSGIMLVAYAAYLVFQLKSDKNLYLPVDEVDNEGSSDDEEAPEISMWESIVW 300

Query: 301 LSILTIWISVLSEYLVNAIEGASVAMDIPVAFISVILLPIVGNAAEHAGAIMFAMKDKLD 360
           LSILTIWISVLSEYLVNAIEGASVAM+IPVAFISVILLPIVGNAAEHAGAIMFAMKDKLD
Sbjct: 301 LSILTIWISVLSEYLVNAIEGASVAMNIPVAFISVILLPIVGNAAEHAGAIMFAMKDKLD 360

Query: 361 ISLGVAIGSSTQISMFGIPFCVVIGWMMGVPMDLNFQLFETATLFITVIVVAFMLQDGTS 420
           ISLGVAIGSSTQISMFGIPFCVVIGW+MG PMDLNFQLFETATLFITVIVVAFMLQDGTS
Sbjct: 361 ISLGVAIGSSTQISMFGIPFCVVIGWIMGCPMDLNFQLFETATLFITVIVVAFMLQDGTS 420

Query: 421 NYLKGLMLILCYLIVAASFFVHIDPASVG 450
           NYLKGLMLILCYLIVAASFFVHIDPASVG
Sbjct: 421 NYLKGLMLILCYLIVAASFFVHIDPASVG 449

BLAST of MS004970 vs. NCBI nr
Match: NP_001267689.1 (vacuolar cation/proton exchanger 5-like [Cucumis sativus] >AFQ99298.1 cation exchanger CAX6 [Cucumis sativus])

HSP 1 Score: 761.5 bits (1965), Expect = 4.0e-216
Identity = 406/448 (90.62%), Postives = 420/448 (93.75%), Query Frame = 0

Query: 1   MDRLRSNDNKQSVERHSHVEMGSLGEKTILEFEDESLVSPVSLNRKNFAFEASMHGSPSS 60
           MD  RSNDNKQ VERHS  EMGSLGEKTILEFEDESLVSPVSL RKNF+ E S+H S   
Sbjct: 1   MDGFRSNDNKQGVERHSRTEMGSLGEKTILEFEDESLVSPVSLTRKNFSIEGSIHSSSLG 60

Query: 61  GDSQKFCYGNMKNRIIDSIKIVVFSAKINLLMPFGPLAILVSSLSGHHGWVFLLSLLGII 120
           G SQK    NMKNR+  SIKIVVFSAKINLLMPFGPLAI+VS+LSGHHGWVFLLSLLGII
Sbjct: 61  GGSQKLSCSNMKNRVFGSIKIVVFSAKINLLMPFGPLAIIVSTLSGHHGWVFLLSLLGII 120

Query: 121 PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALRSGMIRVVQQSLLGSILS 180
           PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALR GMIRVVQQSLLGSILS
Sbjct: 121 PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALRRGMIRVVQQSLLGSILS 180

Query: 181 NMLLVLGCAFFAGGVVVSKREQVFSKGSATVNSGLLLMAVMGLLFPAVLHSTHTELHFGK 240
           NMLLVLGCAFFAGG+VVSKREQVFSK +ATVNSGLLLMAVMGLLFPAVL STHTELH GK
Sbjct: 181 NMLLVLGCAFFAGGIVVSKREQVFSKAAATVNSGLLLMAVMGLLFPAVLRSTHTELHSGK 240

Query: 241 SELALSRFSSGIMLVAYAAYLVFQLKSEKNLYHPVDVVETEENSDDEESPEISMWESIIW 300
           SELALSRFSSGIMLVAYAAYLVFQLKS+KNLY PVD V+ E +SDDEE+PEISMWESI+W
Sbjct: 241 SELALSRFSSGIMLVAYAAYLVFQLKSDKNLYLPVDEVDNEGSSDDEEAPEISMWESIVW 300

Query: 301 LSILTIWISVLSEYLVNAIEGASVAMDIPVAFISVILLPIVGNAAEHAGAIMFAMKDKLD 360
           LSILTIWISVLSEYLVNAIEGASVAM+IPVAFISVILLPIVGNAAEHAGAIMFAMKDKLD
Sbjct: 301 LSILTIWISVLSEYLVNAIEGASVAMNIPVAFISVILLPIVGNAAEHAGAIMFAMKDKLD 360

Query: 361 ISLGVAIGSSTQISMFGIPFCVVIGWMMGVPMDLNFQLFETATLFITVIVVAFMLQDGTS 420
           ISLGVAIGSSTQISMFGIPFCVVIGW+MG PMDLNFQLFETATLFITVIVVAFMLQDGTS
Sbjct: 361 ISLGVAIGSSTQISMFGIPFCVVIGWIMGCPMDLNFQLFETATLFITVIVVAFMLQDGTS 420

Query: 421 NYLKGLMLILCYLIVAASFFVHIDPASV 449
           NYLKGLMLILCYLIVAASFFVHIDPASV
Sbjct: 421 NYLKGLMLILCYLIVAASFFVHIDPASV 448

BLAST of MS004970 vs. NCBI nr
Match: XP_011656306.2 (vacuolar cation/proton exchanger 5-like isoform X4 [Cucumis sativus] >XP_031742351.1 vacuolar cation/proton exchanger 5-like isoform X4 [Cucumis sativus] >XP_031742352.1 vacuolar cation/proton exchanger 5-like isoform X4 [Cucumis sativus])

HSP 1 Score: 761.5 bits (1965), Expect = 4.0e-216
Identity = 406/448 (90.62%), Postives = 420/448 (93.75%), Query Frame = 0

Query: 1   MDRLRSNDNKQSVERHSHVEMGSLGEKTILEFEDESLVSPVSLNRKNFAFEASMHGSPSS 60
           MD  RSNDNKQ VERHS  EMGSLGEKTILEFEDESLVSPVSL RKNF+ E S+H S   
Sbjct: 1   MDGFRSNDNKQGVERHSRTEMGSLGEKTILEFEDESLVSPVSLTRKNFSIEGSIHSSSLG 60

Query: 61  GDSQKFCYGNMKNRIIDSIKIVVFSAKINLLMPFGPLAILVSSLSGHHGWVFLLSLLGII 120
           G SQK    NMKNR+  SIKIVVFSAKINLLMPFGPLAI+VS+LSGHHGWVFLLSLLGII
Sbjct: 61  GGSQKLSCSNMKNRVFGSIKIVVFSAKINLLMPFGPLAIIVSTLSGHHGWVFLLSLLGII 120

Query: 121 PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALRSGMIRVVQQSLLGSILS 180
           PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALR GMIRVVQQSLLGSILS
Sbjct: 121 PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALRRGMIRVVQQSLLGSILS 180

Query: 181 NMLLVLGCAFFAGGVVVSKREQVFSKGSATVNSGLLLMAVMGLLFPAVLHSTHTELHFGK 240
           NMLLVLGCAFFAGG+VVSKREQVFSK +ATVNSGLLLMAVMGLLFPAVL STHTELH GK
Sbjct: 181 NMLLVLGCAFFAGGIVVSKREQVFSKAAATVNSGLLLMAVMGLLFPAVLRSTHTELHSGK 240

Query: 241 SELALSRFSSGIMLVAYAAYLVFQLKSEKNLYHPVDVVETEENSDDEESPEISMWESIIW 300
           SELALSRFSSGIMLVAYAAYLVFQLKS+KNLY PVD V+ E +SDDEE+PEISMWESI+W
Sbjct: 241 SELALSRFSSGIMLVAYAAYLVFQLKSDKNLYLPVDEVDNEGSSDDEEAPEISMWESIVW 300

Query: 301 LSILTIWISVLSEYLVNAIEGASVAMDIPVAFISVILLPIVGNAAEHAGAIMFAMKDKLD 360
           LSILTIWISVLSEYLVNAIEGASVAM+IPVAFISVILLPIVGNAAEHAGAIMFAMKDKLD
Sbjct: 301 LSILTIWISVLSEYLVNAIEGASVAMNIPVAFISVILLPIVGNAAEHAGAIMFAMKDKLD 360

Query: 361 ISLGVAIGSSTQISMFGIPFCVVIGWMMGVPMDLNFQLFETATLFITVIVVAFMLQDGTS 420
           ISLGVAIGSSTQISMFGIPFCVVIGW+MG PMDLNFQLFETATLFITVIVVAFMLQDGTS
Sbjct: 361 ISLGVAIGSSTQISMFGIPFCVVIGWIMGCPMDLNFQLFETATLFITVIVVAFMLQDGTS 420

Query: 421 NYLKGLMLILCYLIVAASFFVHIDPASV 449
           NYLKGLMLILCYLIVAASFFVHIDPASV
Sbjct: 421 NYLKGLMLILCYLIVAASFFVHIDPASV 448

BLAST of MS004970 vs. ExPASy Swiss-Prot
Match: Q8L783 (Vacuolar cation/proton exchanger 5 OS=Arabidopsis thaliana OX=3702 GN=CAX5 PE=2 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 6.2e-159
Identity = 296/375 (78.93%), Postives = 332/375 (88.53%), Query Frame = 0

Query: 73  NRIIDSIKIVVFSAKINLLMPFGPLAILVSSLSGHHGWVFLLSLLGIIPLAERLGYATEQ 132
           N ++ S KIV+ S K+NLL+PFGPLAIL+  L+ + GW+FLLSL+GI PLAERLGYATEQ
Sbjct: 62  NSVLQSFKIVILSNKLNLLLPFGPLAILLHYLTDNKGWIFLLSLVGITPLAERLGYATEQ 121

Query: 133 LACYTGATVGGLLNATFGNATELIISIYALRSGMIRVVQQSLLGSILSNMLLVLGCAFFA 192
           LACYTG+TVGGLLNATFGN TELIISI+AL+SGMIRVVQ +LLGSILSNMLLVLGCAFF 
Sbjct: 122 LACYTGSTVGGLLNATFGNVTELIISIFALKSGMIRVVQLTLLGSILSNMLLVLGCAFFC 181

Query: 193 GGVVVSKREQVFSKGSATVNSGLLLMAVMGLLFPAVLHSTHTELHFGKSELALSRFSSGI 252
           GG+V S++EQVF KG+A VNSGLLLMAVMGLLFPAVLH TH+E+H G SELALSRFSS I
Sbjct: 182 GGLVFSQKEQVFDKGNAVVNSGLLLMAVMGLLFPAVLHYTHSEVHAGSSELALSRFSSCI 241

Query: 253 MLVAYAAYLVFQLKSEKNLYHPV--DVVETEENSDDEESPEISMWESIIWLSILTIWISV 312
           MLVAYAAYL FQLKS+ + Y P+  +  + EE SDD+E PEIS WE+IIWLSILT W+S+
Sbjct: 242 MLVAYAAYLFFQLKSQPSSYTPLTEETNQNEETSDDDEDPEISKWEAIIWLSILTAWVSL 301

Query: 313 LSEYLVNAIEGASVAMDIPVAFISVILLPIVGNAAEHAGAIMFAMKDKLDISLGVAIGSS 372
           LS YLV+AIEGASV+  IP++FISVILLPIVGNAAEHAGAIMFAMKDKLD+SLGVAIGSS
Sbjct: 302 LSGYLVDAIEGASVSWKIPISFISVILLPIVGNAAEHAGAIMFAMKDKLDLSLGVAIGSS 361

Query: 373 TQISMFGIPFCVVIGWMMGVPMDLNFQLFETATLFITVIVVAFMLQDGTSNYLKGLMLIL 432
            QISMF +PFCVVIGWMMG  MDLNFQLFETATLFITVIVVAF LQ+GTSNY KGLMLIL
Sbjct: 362 IQISMFAVPFCVVIGWMMGAQMDLNFQLFETATLFITVIVVAFFLQEGTSNYFKGLMLIL 421

Query: 433 CYLIVAASFFVHIDP 446
           CYLIVAASFFVH DP
Sbjct: 422 CYLIVAASFFVHEDP 436

BLAST of MS004970 vs. ExPASy Swiss-Prot
Match: Q39254 (Vacuolar cation/proton exchanger 2 OS=Arabidopsis thaliana OX=3702 GN=CAX2 PE=1 SV=2)

HSP 1 Score: 553.9 bits (1426), Expect = 1.7e-156
Identity = 293/380 (77.11%), Postives = 330/380 (86.84%), Query Frame = 0

Query: 72  KNRIIDSIKIVVFSAKINLLMPFGPLAILVSSLSGHHGWVFLLSLLGIIPLAERLGYATE 131
           KN +++SIKIV+F  K+NLL+PFGPLAILV  +    GWVFLL+L+GI PLAERLGYATE
Sbjct: 61  KNSVLNSIKIVIFCNKLNLLLPFGPLAILVHYMIDSKGWVFLLTLVGITPLAERLGYATE 120

Query: 132 QLACYTGATVGGLLNATFGNATELIISIYALRSGMIRVVQQSLLGSILSNMLLVLGCAFF 191
           QLACYTG TVGGLLNATFGN TELIISI+AL++GMIRVVQ +LLGSILSNMLLVLGCAFF
Sbjct: 121 QLACYTGPTVGGLLNATFGNVTELIISIFALKNGMIRVVQLTLLGSILSNMLLVLGCAFF 180

Query: 192 AGGVVVSKREQVFSKGSATVNSGLLLMAVMGLLFPAVLHSTHTELHFGKSELALSRFSSG 251
            GG+V  +++QVF KG ATVNSGLLLMAVMG+LFPAVLH TH+E+H G SELALSRFSS 
Sbjct: 181 CGGLVFYQKDQVFDKGIATVNSGLLLMAVMGILFPAVLHYTHSEVHAGSSELALSRFSSC 240

Query: 252 IMLVAYAAYLVFQLKSEKNLYHPVDVV--ETEENSDDEESPEISMWESIIWLSILTIWIS 311
           IML+AYAAYL FQLKS+ N Y P+D    + EE S ++E PEIS WE+IIWLSILT W+S
Sbjct: 241 IMLIAYAAYLFFQLKSQSNSYSPLDEESNQNEETSAEDEDPEISKWEAIIWLSILTAWVS 300

Query: 312 VLSEYLVNAIEGASVAMDIPVAFISVILLPIVGNAAEHAGAIMFAMKDKLDISLGVAIGS 371
           +LS YLV+AIEGASV+ +IP+AFIS ILLPIVGNAAEHAGAIMFAMKDKLD+SLGVAIGS
Sbjct: 301 LLSGYLVDAIEGASVSWNIPIAFISTILLPIVGNAAEHAGAIMFAMKDKLDLSLGVAIGS 360

Query: 372 STQISMFGIPFCVVIGWMMGVPMDLNFQLFETATLFITVIVVAFMLQDGTSNYLKGLMLI 431
           S QISMF +PFCVVIGWMMG  MDLNFQLFETA LFITVIVVAF LQ+G+SNY KGLMLI
Sbjct: 361 SIQISMFAVPFCVVIGWMMGQQMDLNFQLFETAMLFITVIVVAFFLQEGSSNYFKGLMLI 420

Query: 432 LCYLIVAASFFVHIDPASVG 450
           LCYLIVAASFFVH DP   G
Sbjct: 421 LCYLIVAASFFVHEDPHQDG 440

BLAST of MS004970 vs. ExPASy Swiss-Prot
Match: Q5KQN0 (Vacuolar cation/proton exchanger 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CAX2 PE=2 SV=2)

HSP 1 Score: 545.0 bits (1403), Expect = 7.8e-154
Identity = 291/410 (70.98%), Postives = 346/410 (84.39%), Query Frame = 0

Query: 39  SPVSLNRKNFAFEASMHGSPSSGDSQKFCYGNMKNRIIDSIKIVVFSAKINLLMPFGPLA 98
           SP++   K  A +    GS  +  ++    G+   R + S+++V+  AKIN+L+PFGPLA
Sbjct: 25  SPMTAAGKMQALDFEHIGS-LAAVAESLSTGSKWRRALTSVRVVILQAKINVLLPFGPLA 84

Query: 99  ILVSSLSGHH-GWVFLLSLLGIIPLAERLGYATEQLACYTGATVGGLLNATFGNATELII 158
           +++  LS +H GWVFL SL+GI PLAERLGYATEQLA YTG T+GGLLNATFGNATE+II
Sbjct: 85  VMLHYLSANHQGWVFLFSLIGITPLAERLGYATEQLALYTGPTIGGLLNATFGNATEMII 144

Query: 159 SIYALRSGMIRVVQQSLLGSILSNMLLVLGCAFFAGGVVVSKREQVFSKGSATVNSGLLL 218
           S+YAL++GMIRVVQQSLLGSILSNMLLVLGCAFFAGG+V   R+QVF+K SA VNSGLLL
Sbjct: 145 SLYALKNGMIRVVQQSLLGSILSNMLLVLGCAFFAGGLVHPSRDQVFNKASAVVNSGLLL 204

Query: 219 MAVMGLLFPAVLHSTHTELHFGKSELALSRFSSGIMLVAYAAYLVFQLKSEKNLYHPVDV 278
           MAV+GL+FPAVLH TH+E+ +GKSE++LSRFSS IMLVAYA+YL FQLKS+++LY P+  
Sbjct: 205 MAVLGLMFPAVLHFTHSEVQYGKSEVSLSRFSSCIMLVAYASYLFFQLKSQRSLYSPIGE 264

Query: 279 VETEENSDDEESPEISMWESIIWLSILTIWISVLSEYLVNAIEGASVAMDIPVAFISVIL 338
            E E   D+EE  EI+  E+I WL +LTIWIS+LS YLV+AI+GAS ++++PVAFISVIL
Sbjct: 265 QEEEVTEDEEEEKEITQGEAICWLFVLTIWISILSGYLVDAIQGASESLNMPVAFISVIL 324

Query: 339 LPIVGNAAEHAGAIMFAMKDKLDISLGVAIGSSTQISMFGIPFCVVIGWMMGVPMDLNFQ 398
           LPIVGNAAEHA AIMFAMKDKLDI+LGVAIGSSTQISMF IPFCVVIGW+MG  MDLNFQ
Sbjct: 325 LPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFCVVIGWIMGQQMDLNFQ 384

Query: 399 LFETATLFITVIVVAFMLQDGTSNYLKGLMLILCYLIVAASFFVHIDPAS 448
           LFETATLFITV+VVAFMLQ+GTSNY KGLMLILCYLIVAASFFVH+DP S
Sbjct: 385 LFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFVHVDPDS 433

BLAST of MS004970 vs. ExPASy Swiss-Prot
Match: Q6K1C4 (Vacuolar cation/proton exchanger 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CAX3 PE=2 SV=2)

HSP 1 Score: 537.3 bits (1383), Expect = 1.6e-151
Identity = 280/372 (75.27%), Postives = 327/372 (87.90%), Query Frame = 0

Query: 78  SIKIVVFSAKINLLMPFGPLAILVSSLSGHHGWVFLLSLLGIIPLAERLGYATEQLACYT 137
           SI+IV+F++K+NLL+PFGP +I++   +  HG VFL S+LGI PLAERLGYATEQLA YT
Sbjct: 43  SIRIVIFTSKLNLLLPFGPASIILHYTTSRHGLVFLFSMLGITPLAERLGYATEQLAIYT 102

Query: 138 GATVGGLLNATFGNATELIISIYALRSGMIRVVQQSLLGSILSNMLLVLGCAFFAGGVVV 197
           G TVGGLLNATFGNATE+II+IYAL++GMIRVVQQSLLGSILSNMLLV+GCAFFAGG+V 
Sbjct: 103 GPTVGGLLNATFGNATEMIIAIYALKNGMIRVVQQSLLGSILSNMLLVMGCAFFAGGIVH 162

Query: 198 SKREQVFSKGSATVNSGLLLMAVMGLLFPAVLHSTHTELHFGKSELALSRFSSGIMLVAY 257
             ++QVFSK +A VNSGLLLMAVMGL+FPAVLH TH+E+  G SE++LSRFSS IMLVAY
Sbjct: 163 RNKDQVFSKATAVVNSGLLLMAVMGLMFPAVLHFTHSEVRQGASEVSLSRFSSCIMLVAY 222

Query: 258 AAYLVFQLKSEKNLYHPV--DVVETEENSDDEESPEISMWESIIWLSILTIWISVLSEYL 317
           A+YL FQL    N Y P+  + +  E+ ++++E  EI MWESI WL++LT+W+S+LSEYL
Sbjct: 223 ASYLYFQLSGRNNAYSPIGSEEMPNEDAAEEDEESEIGMWESIAWLAMLTLWVSILSEYL 282

Query: 318 VNAIEGASVAMDIPVAFISVILLPIVGNAAEHAGAIMFAMKDKLDISLGVAIGSSTQISM 377
           VNAIEGAS ++++PVAFISVILLPIVGNAAEHA AIMFAMKDKLDI+LGVAIGSSTQISM
Sbjct: 283 VNAIEGASDSLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISM 342

Query: 378 FGIPFCVVIGWMMGVPMDLNFQLFETATLFITVIVVAFMLQDGTSNYLKGLMLILCYLIV 437
           F IPFCVVIGWMMG  MDLNFQLFETATLFITV+VVAFMLQDG +NYLKGLMLILCYLIV
Sbjct: 343 FVIPFCVVIGWMMGQKMDLNFQLFETATLFITVLVVAFMLQDGVANYLKGLMLILCYLIV 402

Query: 438 AASFFVHIDPAS 448
           AASFFVH+DP S
Sbjct: 403 AASFFVHVDPQS 414

BLAST of MS004970 vs. ExPASy Swiss-Prot
Match: Q9LFZ8 (Putative vacuolar cation/proton exchanger 6 OS=Arabidopsis thaliana OX=3702 GN=CAX6 PE=3 SV=3)

HSP 1 Score: 519.6 bits (1337), Expect = 3.5e-146
Identity = 279/375 (74.40%), Postives = 316/375 (84.27%), Query Frame = 0

Query: 72  KNRIIDSIKIVVFSAKINLLMPFGPLAILVSSLSGHHGWVFLLSLLGIIPLAERLGYATE 131
           +N ++ S KIV+ S K+NLL+PFGPLAILV  L+ + GW FLLSL+GI PLAERLGYATE
Sbjct: 72  RNSVVQSFKIVILSNKLNLLLPFGPLAILVHYLTDNKGWFFLLSLVGITPLAERLGYATE 131

Query: 132 QLACYTGATVGGLLNATFGNATELIISIYALRSGMIRVVQQSLLGSILSNMLLVLGCAFF 191
           QL+CYTGATVGGLLNATFGN  ELIISI AL++GMIRVVQ +LLGSILSN+LLVLGCAFF
Sbjct: 132 QLSCYTGATVGGLLNATFGNVIELIISIIALKNGMIRVVQLTLLGSILSNILLVLGCAFF 191

Query: 192 AGGVVVSKREQVFSKGSATVNSGLLLMAVMGLLFPAVLHSTHTELHFGKSELALSRFSSG 251
            GG+V   ++QVF K +A V+SG+LLMAVMGLLFP  LH TH+E+H G SELALSRF S 
Sbjct: 192 CGGLVFPGKDQVFDKRNAVVSSGMLLMAVMGLLFPTFLHYTHSEVHAGSSELALSRFISC 251

Query: 252 IMLVAYAAYLVFQLKSEKNLYHPVDVVETEENSDDEESPEISMWESIIWLSILTIWISVL 311
           IMLVAYAAYL FQLKS+ + Y      + EE S+D+E PEIS WE+IIWLSI T W+S+L
Sbjct: 252 IMLVAYAAYLFFQLKSQPSFY-TEKTNQNEETSNDDEDPEISKWEAIIWLSIFTAWVSLL 311

Query: 312 SEYLVNAIEGASVAMDIPVAFISVILLPIVGNAAEHAGAIMFAMKDKLDISLGVAIGSST 371
           S YLV+AIEG SV+  IP++FISVILLPIVGNAAEHAGAIMFAMKDKLD+SLGVAIGSS 
Sbjct: 312 SGYLVDAIEGTSVSWKIPISFISVILLPIVGNAAEHAGAIMFAMKDKLDLSLGVAIGSSI 371

Query: 372 QISMFGIPFCVVIGWMMGVPMDLNFQLFETATLFITVIVVAFMLQ-DGTSNYLKGLMLIL 431
           QISMF +PFCVVIGWMMG  MDLN QLFETATL ITVIVVAF LQ +GTSNY K LMLIL
Sbjct: 372 QISMFAVPFCVVIGWMMGAQMDLNLQLFETATLLITVIVVAFFLQLEGTSNYFKRLMLIL 431

Query: 432 CYLIVAASFFVHIDP 446
           CYLIVAASFFVH DP
Sbjct: 432 CYLIVAASFFVHEDP 445

BLAST of MS004970 vs. ExPASy TrEMBL
Match: A0A6J1CF97 (Vacuolar cation/proton exchanger OS=Momordica charantia OX=3673 GN=LOC111011152 PE=3 SV=1)

HSP 1 Score: 837.4 bits (2162), Expect = 2.8e-239
Identity = 447/451 (99.11%), Postives = 447/451 (99.11%), Query Frame = 0

Query: 1   MDRLRSNDNKQSVERHSHVEMGSLGEKTILEFEDESLVSPVSLNRKNFAFEASMHGSPSS 60
           MDRL SNDNKQSVERHSHVEMGSLGEKTILEFEDESLVSPVSLNRKNFAFEASMHGSPSS
Sbjct: 1   MDRLGSNDNKQSVERHSHVEMGSLGEKTILEFEDESLVSPVSLNRKNFAFEASMHGSPSS 60

Query: 61  GDSQKFCYGNMKNRIIDSIKIVVFSAKINLLMPFGPLAILVSSLSGHHGWVFLLSLLGII 120
           GDSQKFCYGNMKNRIIDSIKIVVFSAKINLLMPFGPLAILVSSLSGHHGWVFLLSLLGII
Sbjct: 61  GDSQKFCYGNMKNRIIDSIKIVVFSAKINLLMPFGPLAILVSSLSGHHGWVFLLSLLGII 120

Query: 121 PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALRSGMIRVVQQSLLGSILS 180
           PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALRSGMIRVVQQSLLGSILS
Sbjct: 121 PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALRSGMIRVVQQSLLGSILS 180

Query: 181 NMLLVLGCAFFAGGVVVSKREQVFSKGSATVNSGLLLMAVMGLLFPAVLHSTHTELHFGK 240
           NMLLVLGCAFFAGGVVVSKREQVFSKGSATVNSGLLLMAVMGLLFPAVLHSTHTELHFGK
Sbjct: 181 NMLLVLGCAFFAGGVVVSKREQVFSKGSATVNSGLLLMAVMGLLFPAVLHSTHTELHFGK 240

Query: 241 SELALSRFSSGIMLVAYAAYLVFQLKSEKNLYHPVDV---VETEENSDDEESPEISMWES 300
           SELALSRFSSGIMLVAYAAYLVFQLKSEKNLYHPVDV   VETEENSDDEESPEISMWES
Sbjct: 241 SELALSRFSSGIMLVAYAAYLVFQLKSEKNLYHPVDVVDEVETEENSDDEESPEISMWES 300

Query: 301 IIWLSILTIWISVLSEYLVNAIEGASVAMDIPVAFISVILLPIVGNAAEHAGAIMFAMKD 360
           IIWLSILTIWISVLSEYLVNAIEGASVAMDIPVAFISVILLPIVGNAAEHAGAIMFAMKD
Sbjct: 301 IIWLSILTIWISVLSEYLVNAIEGASVAMDIPVAFISVILLPIVGNAAEHAGAIMFAMKD 360

Query: 361 KLDISLGVAIGSSTQISMFGIPFCVVIGWMMGVPMDLNFQLFETATLFITVIVVAFMLQD 420
           KLDISLGVAIGSSTQISMFGIPFCVVIGWMMGVPMDLNFQLFETATLFITVIVVAFMLQD
Sbjct: 361 KLDISLGVAIGSSTQISMFGIPFCVVIGWMMGVPMDLNFQLFETATLFITVIVVAFMLQD 420

Query: 421 GTSNYLKGLMLILCYLIVAASFFVHIDPASV 449
           GTSNYLKGLMLILCYLIVAASFFVHIDPASV
Sbjct: 421 GTSNYLKGLMLILCYLIVAASFFVHIDPASV 451

BLAST of MS004970 vs. ExPASy TrEMBL
Match: A0A1S3BJ06 (Vacuolar cation/proton exchanger OS=Cucumis melo OX=3656 GN=LOC103490425 PE=3 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 5.0e-220
Identity = 410/449 (91.31%), Postives = 424/449 (94.43%), Query Frame = 0

Query: 1   MDRLRSNDNKQSVERHSHVEMGSLGEKTILEFEDESLVSPVSLNRKNFAFEASMHGSPSS 60
           MD  RSNDNKQ VERHS  EMGSLGEKTILEFEDESLVSPVSLNRKNF+ E S+H SP  
Sbjct: 1   MDGFRSNDNKQGVERHSRTEMGSLGEKTILEFEDESLVSPVSLNRKNFSIEGSIHSSPLG 60

Query: 61  GDSQKFCYGNMKNRIIDSIKIVVFSAKINLLMPFGPLAILVSSLSGHHGWVFLLSLLGII 120
           G SQK C  NMKNR+  SIKIVVFSAKINLLMPFGPLAI+VS+LSGHHGWVFLLSLLGII
Sbjct: 61  GGSQKLCCSNMKNRVFGSIKIVVFSAKINLLMPFGPLAIIVSTLSGHHGWVFLLSLLGII 120

Query: 121 PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALRSGMIRVVQQSLLGSILS 180
           PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALR GMIRVVQQSLLGSILS
Sbjct: 121 PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALRRGMIRVVQQSLLGSILS 180

Query: 181 NMLLVLGCAFFAGGVVVSKREQVFSKGSATVNSGLLLMAVMGLLFPAVLHSTHTELHFGK 240
           NMLLVLGCAFFAGG+VVSKREQVFSK +ATVNSGLLLMAVMGLLFPAVL STHTELH GK
Sbjct: 181 NMLLVLGCAFFAGGIVVSKREQVFSKAAATVNSGLLLMAVMGLLFPAVLRSTHTELHSGK 240

Query: 241 SELALSRFSSGIMLVAYAAYLVFQLKSEKNLYHPVDVVETEENSDDEESPEISMWESIIW 300
           SELALSRFSSGIMLVAYAAYLVFQLKS+KNLY PVD V+ E +SDDEE+PEISMWESI+W
Sbjct: 241 SELALSRFSSGIMLVAYAAYLVFQLKSDKNLYLPVDEVDNEGSSDDEEAPEISMWESIVW 300

Query: 301 LSILTIWISVLSEYLVNAIEGASVAMDIPVAFISVILLPIVGNAAEHAGAIMFAMKDKLD 360
           LSILTIWISVLSEYLVNAIEGASVAM+IPVAFISVILLPIVGNAAEHAGAIMFAMKDKLD
Sbjct: 301 LSILTIWISVLSEYLVNAIEGASVAMNIPVAFISVILLPIVGNAAEHAGAIMFAMKDKLD 360

Query: 361 ISLGVAIGSSTQISMFGIPFCVVIGWMMGVPMDLNFQLFETATLFITVIVVAFMLQDGTS 420
           ISLGVAIGSSTQISMFGIPFCVVIGW+MG PMDLNFQLFETATLFITVIVVAFMLQDGTS
Sbjct: 361 ISLGVAIGSSTQISMFGIPFCVVIGWIMGCPMDLNFQLFETATLFITVIVVAFMLQDGTS 420

Query: 421 NYLKGLMLILCYLIVAASFFVHIDPASVG 450
           NYLKGLMLILCYLIVAASFFVHIDPASVG
Sbjct: 421 NYLKGLMLILCYLIVAASFFVHIDPASVG 449

BLAST of MS004970 vs. ExPASy TrEMBL
Match: A0A0A0KCR2 (Vacuolar cation/proton exchanger OS=Cucumis sativus OX=3659 GN=Csa_6G115100 PE=3 SV=1)

HSP 1 Score: 764.2 bits (1972), Expect = 3.0e-217
Identity = 407/449 (90.65%), Postives = 421/449 (93.76%), Query Frame = 0

Query: 1   MDRLRSNDNKQSVERHSHVEMGSLGEKTILEFEDESLVSPVSLNRKNFAFEASMHGSPSS 60
           MD  RSNDNKQ VERHS  EMGSLGEKTILEFEDESLVSPVSL RKNF+ E S+H S   
Sbjct: 1   MDGFRSNDNKQGVERHSRTEMGSLGEKTILEFEDESLVSPVSLTRKNFSIEGSIHSSSLG 60

Query: 61  GDSQKFCYGNMKNRIIDSIKIVVFSAKINLLMPFGPLAILVSSLSGHHGWVFLLSLLGII 120
           G SQK    NMKNR+  SIKIVVFSAKINLLMPFGPLAI+VS+LSGHHGWVFLLSLLGII
Sbjct: 61  GGSQKLSCSNMKNRVFGSIKIVVFSAKINLLMPFGPLAIIVSTLSGHHGWVFLLSLLGII 120

Query: 121 PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALRSGMIRVVQQSLLGSILS 180
           PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALR GMIRVVQQSLLGSILS
Sbjct: 121 PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALRRGMIRVVQQSLLGSILS 180

Query: 181 NMLLVLGCAFFAGGVVVSKREQVFSKGSATVNSGLLLMAVMGLLFPAVLHSTHTELHFGK 240
           NMLLVLGCAFFAGG+VVSKREQVFSK +ATVNSGLLLMAVMGLLFPAVL STHTELH GK
Sbjct: 181 NMLLVLGCAFFAGGIVVSKREQVFSKAAATVNSGLLLMAVMGLLFPAVLRSTHTELHSGK 240

Query: 241 SELALSRFSSGIMLVAYAAYLVFQLKSEKNLYHPVDVVETEENSDDEESPEISMWESIIW 300
           SELALSRFSSGIMLVAYAAYLVFQLKS+KNLY PVD V+ E +SDDEE+PEISMWESI+W
Sbjct: 241 SELALSRFSSGIMLVAYAAYLVFQLKSDKNLYLPVDEVDNEGSSDDEEAPEISMWESIVW 300

Query: 301 LSILTIWISVLSEYLVNAIEGASVAMDIPVAFISVILLPIVGNAAEHAGAIMFAMKDKLD 360
           LSILTIWISVLSEYLVNAIEGASVAM+IPVAFISVILLPIVGNAAEHAGAIMFAMKDKLD
Sbjct: 301 LSILTIWISVLSEYLVNAIEGASVAMNIPVAFISVILLPIVGNAAEHAGAIMFAMKDKLD 360

Query: 361 ISLGVAIGSSTQISMFGIPFCVVIGWMMGVPMDLNFQLFETATLFITVIVVAFMLQDGTS 420
           ISLGVAIGSSTQISMFGIPFCVVIGW+MG PMDLNFQLFETATLFITVIVVAFMLQDGTS
Sbjct: 361 ISLGVAIGSSTQISMFGIPFCVVIGWIMGCPMDLNFQLFETATLFITVIVVAFMLQDGTS 420

Query: 421 NYLKGLMLILCYLIVAASFFVHIDPASVG 450
           NYLKGLMLILCYLIVAASFFVHIDPASVG
Sbjct: 421 NYLKGLMLILCYLIVAASFFVHIDPASVG 449

BLAST of MS004970 vs. ExPASy TrEMBL
Match: J7KEC3 (Vacuolar cation/proton exchanger OS=Cucumis sativus OX=3659 GN=CAX6 PE=2 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 2.0e-216
Identity = 406/448 (90.62%), Postives = 420/448 (93.75%), Query Frame = 0

Query: 1   MDRLRSNDNKQSVERHSHVEMGSLGEKTILEFEDESLVSPVSLNRKNFAFEASMHGSPSS 60
           MD  RSNDNKQ VERHS  EMGSLGEKTILEFEDESLVSPVSL RKNF+ E S+H S   
Sbjct: 1   MDGFRSNDNKQGVERHSRTEMGSLGEKTILEFEDESLVSPVSLTRKNFSIEGSIHSSSLG 60

Query: 61  GDSQKFCYGNMKNRIIDSIKIVVFSAKINLLMPFGPLAILVSSLSGHHGWVFLLSLLGII 120
           G SQK    NMKNR+  SIKIVVFSAKINLLMPFGPLAI+VS+LSGHHGWVFLLSLLGII
Sbjct: 61  GGSQKLSCSNMKNRVFGSIKIVVFSAKINLLMPFGPLAIIVSTLSGHHGWVFLLSLLGII 120

Query: 121 PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALRSGMIRVVQQSLLGSILS 180
           PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALR GMIRVVQQSLLGSILS
Sbjct: 121 PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALRRGMIRVVQQSLLGSILS 180

Query: 181 NMLLVLGCAFFAGGVVVSKREQVFSKGSATVNSGLLLMAVMGLLFPAVLHSTHTELHFGK 240
           NMLLVLGCAFFAGG+VVSKREQVFSK +ATVNSGLLLMAVMGLLFPAVL STHTELH GK
Sbjct: 181 NMLLVLGCAFFAGGIVVSKREQVFSKAAATVNSGLLLMAVMGLLFPAVLRSTHTELHSGK 240

Query: 241 SELALSRFSSGIMLVAYAAYLVFQLKSEKNLYHPVDVVETEENSDDEESPEISMWESIIW 300
           SELALSRFSSGIMLVAYAAYLVFQLKS+KNLY PVD V+ E +SDDEE+PEISMWESI+W
Sbjct: 241 SELALSRFSSGIMLVAYAAYLVFQLKSDKNLYLPVDEVDNEGSSDDEEAPEISMWESIVW 300

Query: 301 LSILTIWISVLSEYLVNAIEGASVAMDIPVAFISVILLPIVGNAAEHAGAIMFAMKDKLD 360
           LSILTIWISVLSEYLVNAIEGASVAM+IPVAFISVILLPIVGNAAEHAGAIMFAMKDKLD
Sbjct: 301 LSILTIWISVLSEYLVNAIEGASVAMNIPVAFISVILLPIVGNAAEHAGAIMFAMKDKLD 360

Query: 361 ISLGVAIGSSTQISMFGIPFCVVIGWMMGVPMDLNFQLFETATLFITVIVVAFMLQDGTS 420
           ISLGVAIGSSTQISMFGIPFCVVIGW+MG PMDLNFQLFETATLFITVIVVAFMLQDGTS
Sbjct: 361 ISLGVAIGSSTQISMFGIPFCVVIGWIMGCPMDLNFQLFETATLFITVIVVAFMLQDGTS 420

Query: 421 NYLKGLMLILCYLIVAASFFVHIDPASV 449
           NYLKGLMLILCYLIVAASFFVHIDPASV
Sbjct: 421 NYLKGLMLILCYLIVAASFFVHIDPASV 448

BLAST of MS004970 vs. ExPASy TrEMBL
Match: A0A6J1FW92 (Vacuolar cation/proton exchanger OS=Cucurbita moschata OX=3662 GN=LOC111449068 PE=3 SV=1)

HSP 1 Score: 754.6 bits (1947), Expect = 2.4e-214
Identity = 401/448 (89.51%), Postives = 419/448 (93.53%), Query Frame = 0

Query: 1   MDRLRSNDNKQSVERHSHVEMGSLGEKTILEFEDESLVSPVSLNRKNFAFEASMHGSPSS 60
           MD LRSNDNKQ+++RHS  EMGS  E+TILEFEDESLVSPVSLNRKNF+ E   H S   
Sbjct: 1   MDGLRSNDNKQNIDRHSRAEMGSFSERTILEFEDESLVSPVSLNRKNFSIE---HSSSLG 60

Query: 61  GDSQKFCYGNMKNRIIDSIKIVVFSAKINLLMPFGPLAILVSSLSGHHGWVFLLSLLGII 120
           G SQK CYGNMKNR+  S+K+VVFSAKIN LMPFGPLAILVSSL+GHHGWVFLLSLLGII
Sbjct: 61  GGSQKLCYGNMKNRVFHSLKVVVFSAKINFLMPFGPLAILVSSLTGHHGWVFLLSLLGII 120

Query: 121 PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALRSGMIRVVQQSLLGSILS 180
           PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALR GMIRVVQQSLLGSILS
Sbjct: 121 PLAERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALRRGMIRVVQQSLLGSILS 180

Query: 181 NMLLVLGCAFFAGGVVVSKREQVFSKGSATVNSGLLLMAVMGLLFPAVLHSTHTELHFGK 240
           NMLLVLGCAFFAGG+VVSKREQVFSK SATVNSGLLLMAVMGLLFPAVL STHTELH GK
Sbjct: 181 NMLLVLGCAFFAGGIVVSKREQVFSKASATVNSGLLLMAVMGLLFPAVLRSTHTELHSGK 240

Query: 241 SELALSRFSSGIMLVAYAAYLVFQLKSEKNLYHPVDVVETEENSDDEESPEISMWESIIW 300
           SELALSRFSSGIMLVAYAAYLVFQLKS+KNLY PV   +TEE+ DDE++PEISMWESIIW
Sbjct: 241 SELALSRFSSGIMLVAYAAYLVFQLKSDKNLYLPVTEEDTEESLDDEDAPEISMWESIIW 300

Query: 301 LSILTIWISVLSEYLVNAIEGASVAMDIPVAFISVILLPIVGNAAEHAGAIMFAMKDKLD 360
           L ILTIWISVLSEYLVNAIEGASVAM+IPVAFISVILLPIVGNAAEHAGAIMFAMKDKLD
Sbjct: 301 LFILTIWISVLSEYLVNAIEGASVAMNIPVAFISVILLPIVGNAAEHAGAIMFAMKDKLD 360

Query: 361 ISLGVAIGSSTQISMFGIPFCVVIGWMMGVPMDLNFQLFETATLFITVIVVAFMLQDGTS 420
           ISLGVAIGSSTQISMFGIPFCVVIGW+MG PMDLNFQLFETATLFITVIVVAFMLQDGTS
Sbjct: 361 ISLGVAIGSSTQISMFGIPFCVVIGWIMGCPMDLNFQLFETATLFITVIVVAFMLQDGTS 420

Query: 421 NYLKGLMLILCYLIVAASFFVHIDPASV 449
           NYLKGLMLILCYLIVAASFFVH+DPASV
Sbjct: 421 NYLKGLMLILCYLIVAASFFVHVDPASV 445

BLAST of MS004970 vs. TAIR 10
Match: AT1G55730.1 (cation exchanger 5 )

HSP 1 Score: 562.0 bits (1447), Expect = 4.4e-160
Identity = 296/375 (78.93%), Postives = 332/375 (88.53%), Query Frame = 0

Query: 73  NRIIDSIKIVVFSAKINLLMPFGPLAILVSSLSGHHGWVFLLSLLGIIPLAERLGYATEQ 132
           N ++ S KIV+ S K+NLL+PFGPLAIL+  L+ + GW+FLLSL+GI PLAERLGYATEQ
Sbjct: 62  NSVLQSFKIVILSNKLNLLLPFGPLAILLHYLTDNKGWIFLLSLVGITPLAERLGYATEQ 121

Query: 133 LACYTGATVGGLLNATFGNATELIISIYALRSGMIRVVQQSLLGSILSNMLLVLGCAFFA 192
           LACYTG+TVGGLLNATFGN TELIISI+AL+SGMIRVVQ +LLGSILSNMLLVLGCAFF 
Sbjct: 122 LACYTGSTVGGLLNATFGNVTELIISIFALKSGMIRVVQLTLLGSILSNMLLVLGCAFFC 181

Query: 193 GGVVVSKREQVFSKGSATVNSGLLLMAVMGLLFPAVLHSTHTELHFGKSELALSRFSSGI 252
           GG+V S++EQVF KG+A VNSGLLLMAVMGLLFPAVLH TH+E+H G SELALSRFSS I
Sbjct: 182 GGLVFSQKEQVFDKGNAVVNSGLLLMAVMGLLFPAVLHYTHSEVHAGSSELALSRFSSCI 241

Query: 253 MLVAYAAYLVFQLKSEKNLYHPV--DVVETEENSDDEESPEISMWESIIWLSILTIWISV 312
           MLVAYAAYL FQLKS+ + Y P+  +  + EE SDD+E PEIS WE+IIWLSILT W+S+
Sbjct: 242 MLVAYAAYLFFQLKSQPSSYTPLTEETNQNEETSDDDEDPEISKWEAIIWLSILTAWVSL 301

Query: 313 LSEYLVNAIEGASVAMDIPVAFISVILLPIVGNAAEHAGAIMFAMKDKLDISLGVAIGSS 372
           LS YLV+AIEGASV+  IP++FISVILLPIVGNAAEHAGAIMFAMKDKLD+SLGVAIGSS
Sbjct: 302 LSGYLVDAIEGASVSWKIPISFISVILLPIVGNAAEHAGAIMFAMKDKLDLSLGVAIGSS 361

Query: 373 TQISMFGIPFCVVIGWMMGVPMDLNFQLFETATLFITVIVVAFMLQDGTSNYLKGLMLIL 432
            QISMF +PFCVVIGWMMG  MDLNFQLFETATLFITVIVVAF LQ+GTSNY KGLMLIL
Sbjct: 362 IQISMFAVPFCVVIGWMMGAQMDLNFQLFETATLFITVIVVAFFLQEGTSNYFKGLMLIL 421

Query: 433 CYLIVAASFFVHIDP 446
           CYLIVAASFFVH DP
Sbjct: 422 CYLIVAASFFVHEDP 436

BLAST of MS004970 vs. TAIR 10
Match: AT1G55730.2 (cation exchanger 5 )

HSP 1 Score: 562.0 bits (1447), Expect = 4.4e-160
Identity = 296/375 (78.93%), Postives = 332/375 (88.53%), Query Frame = 0

Query: 73  NRIIDSIKIVVFSAKINLLMPFGPLAILVSSLSGHHGWVFLLSLLGIIPLAERLGYATEQ 132
           N ++ S KIV+ S K+NLL+PFGPLAIL+  L+ + GW+FLLSL+GI PLAERLGYATEQ
Sbjct: 62  NSVLQSFKIVILSNKLNLLLPFGPLAILLHYLTDNKGWIFLLSLVGITPLAERLGYATEQ 121

Query: 133 LACYTGATVGGLLNATFGNATELIISIYALRSGMIRVVQQSLLGSILSNMLLVLGCAFFA 192
           LACYTG+TVGGLLNATFGN TELIISI+AL+SGMIRVVQ +LLGSILSNMLLVLGCAFF 
Sbjct: 122 LACYTGSTVGGLLNATFGNVTELIISIFALKSGMIRVVQLTLLGSILSNMLLVLGCAFFC 181

Query: 193 GGVVVSKREQVFSKGSATVNSGLLLMAVMGLLFPAVLHSTHTELHFGKSELALSRFSSGI 252
           GG+V S++EQVF KG+A VNSGLLLMAVMGLLFPAVLH TH+E+H G SELALSRFSS I
Sbjct: 182 GGLVFSQKEQVFDKGNAVVNSGLLLMAVMGLLFPAVLHYTHSEVHAGSSELALSRFSSCI 241

Query: 253 MLVAYAAYLVFQLKSEKNLYHPV--DVVETEENSDDEESPEISMWESIIWLSILTIWISV 312
           MLVAYAAYL FQLKS+ + Y P+  +  + EE SDD+E PEIS WE+IIWLSILT W+S+
Sbjct: 242 MLVAYAAYLFFQLKSQPSSYTPLTEETNQNEETSDDDEDPEISKWEAIIWLSILTAWVSL 301

Query: 313 LSEYLVNAIEGASVAMDIPVAFISVILLPIVGNAAEHAGAIMFAMKDKLDISLGVAIGSS 372
           LS YLV+AIEGASV+  IP++FISVILLPIVGNAAEHAGAIMFAMKDKLD+SLGVAIGSS
Sbjct: 302 LSGYLVDAIEGASVSWKIPISFISVILLPIVGNAAEHAGAIMFAMKDKLDLSLGVAIGSS 361

Query: 373 TQISMFGIPFCVVIGWMMGVPMDLNFQLFETATLFITVIVVAFMLQDGTSNYLKGLMLIL 432
            QISMF +PFCVVIGWMMG  MDLNFQLFETATLFITVIVVAF LQ+GTSNY KGLMLIL
Sbjct: 362 IQISMFAVPFCVVIGWMMGAQMDLNFQLFETATLFITVIVVAFFLQEGTSNYFKGLMLIL 421

Query: 433 CYLIVAASFFVHIDP 446
           CYLIVAASFFVH DP
Sbjct: 422 CYLIVAASFFVHEDP 436

BLAST of MS004970 vs. TAIR 10
Match: AT3G13320.1 (cation exchanger 2 )

HSP 1 Score: 553.9 bits (1426), Expect = 1.2e-157
Identity = 293/380 (77.11%), Postives = 330/380 (86.84%), Query Frame = 0

Query: 72  KNRIIDSIKIVVFSAKINLLMPFGPLAILVSSLSGHHGWVFLLSLLGIIPLAERLGYATE 131
           KN +++SIKIV+F  K+NLL+PFGPLAILV  +    GWVFLL+L+GI PLAERLGYATE
Sbjct: 61  KNSVLNSIKIVIFCNKLNLLLPFGPLAILVHYMIDSKGWVFLLTLVGITPLAERLGYATE 120

Query: 132 QLACYTGATVGGLLNATFGNATELIISIYALRSGMIRVVQQSLLGSILSNMLLVLGCAFF 191
           QLACYTG TVGGLLNATFGN TELIISI+AL++GMIRVVQ +LLGSILSNMLLVLGCAFF
Sbjct: 121 QLACYTGPTVGGLLNATFGNVTELIISIFALKNGMIRVVQLTLLGSILSNMLLVLGCAFF 180

Query: 192 AGGVVVSKREQVFSKGSATVNSGLLLMAVMGLLFPAVLHSTHTELHFGKSELALSRFSSG 251
            GG+V  +++QVF KG ATVNSGLLLMAVMG+LFPAVLH TH+E+H G SELALSRFSS 
Sbjct: 181 CGGLVFYQKDQVFDKGIATVNSGLLLMAVMGILFPAVLHYTHSEVHAGSSELALSRFSSC 240

Query: 252 IMLVAYAAYLVFQLKSEKNLYHPVDVV--ETEENSDDEESPEISMWESIIWLSILTIWIS 311
           IML+AYAAYL FQLKS+ N Y P+D    + EE S ++E PEIS WE+IIWLSILT W+S
Sbjct: 241 IMLIAYAAYLFFQLKSQSNSYSPLDEESNQNEETSAEDEDPEISKWEAIIWLSILTAWVS 300

Query: 312 VLSEYLVNAIEGASVAMDIPVAFISVILLPIVGNAAEHAGAIMFAMKDKLDISLGVAIGS 371
           +LS YLV+AIEGASV+ +IP+AFIS ILLPIVGNAAEHAGAIMFAMKDKLD+SLGVAIGS
Sbjct: 301 LLSGYLVDAIEGASVSWNIPIAFISTILLPIVGNAAEHAGAIMFAMKDKLDLSLGVAIGS 360

Query: 372 STQISMFGIPFCVVIGWMMGVPMDLNFQLFETATLFITVIVVAFMLQDGTSNYLKGLMLI 431
           S QISMF +PFCVVIGWMMG  MDLNFQLFETA LFITVIVVAF LQ+G+SNY KGLMLI
Sbjct: 361 SIQISMFAVPFCVVIGWMMGQQMDLNFQLFETAMLFITVIVVAFFLQEGSSNYFKGLMLI 420

Query: 432 LCYLIVAASFFVHIDPASVG 450
           LCYLIVAASFFVH DP   G
Sbjct: 421 LCYLIVAASFFVHEDPHQDG 440

BLAST of MS004970 vs. TAIR 10
Match: AT1G55720.1 (cation exchanger 6 )

HSP 1 Score: 519.6 bits (1337), Expect = 2.5e-147
Identity = 279/375 (74.40%), Postives = 316/375 (84.27%), Query Frame = 0

Query: 72  KNRIIDSIKIVVFSAKINLLMPFGPLAILVSSLSGHHGWVFLLSLLGIIPLAERLGYATE 131
           +N ++ S KIV+ S K+NLL+PFGPLAILV  L+ + GW FLLSL+GI PLAERLGYATE
Sbjct: 72  RNSVVQSFKIVILSNKLNLLLPFGPLAILVHYLTDNKGWFFLLSLVGITPLAERLGYATE 131

Query: 132 QLACYTGATVGGLLNATFGNATELIISIYALRSGMIRVVQQSLLGSILSNMLLVLGCAFF 191
           QL+CYTGATVGGLLNATFGN  ELIISI AL++GMIRVVQ +LLGSILSN+LLVLGCAFF
Sbjct: 132 QLSCYTGATVGGLLNATFGNVIELIISIIALKNGMIRVVQLTLLGSILSNILLVLGCAFF 191

Query: 192 AGGVVVSKREQVFSKGSATVNSGLLLMAVMGLLFPAVLHSTHTELHFGKSELALSRFSSG 251
            GG+V   ++QVF K +A V+SG+LLMAVMGLLFP  LH TH+E+H G SELALSRF S 
Sbjct: 192 CGGLVFPGKDQVFDKRNAVVSSGMLLMAVMGLLFPTFLHYTHSEVHAGSSELALSRFISC 251

Query: 252 IMLVAYAAYLVFQLKSEKNLYHPVDVVETEENSDDEESPEISMWESIIWLSILTIWISVL 311
           IMLVAYAAYL FQLKS+ + Y      + EE S+D+E PEIS WE+IIWLSI T W+S+L
Sbjct: 252 IMLVAYAAYLFFQLKSQPSFY-TEKTNQNEETSNDDEDPEISKWEAIIWLSIFTAWVSLL 311

Query: 312 SEYLVNAIEGASVAMDIPVAFISVILLPIVGNAAEHAGAIMFAMKDKLDISLGVAIGSST 371
           S YLV+AIEG SV+  IP++FISVILLPIVGNAAEHAGAIMFAMKDKLD+SLGVAIGSS 
Sbjct: 312 SGYLVDAIEGTSVSWKIPISFISVILLPIVGNAAEHAGAIMFAMKDKLDLSLGVAIGSSI 371

Query: 372 QISMFGIPFCVVIGWMMGVPMDLNFQLFETATLFITVIVVAFMLQ-DGTSNYLKGLMLIL 431
           QISMF +PFCVVIGWMMG  MDLN QLFETATL ITVIVVAF LQ +GTSNY K LMLIL
Sbjct: 372 QISMFAVPFCVVIGWMMGAQMDLNLQLFETATLLITVIVVAFFLQLEGTSNYFKRLMLIL 431

Query: 432 CYLIVAASFFVHIDP 446
           CYLIVAASFFVH DP
Sbjct: 432 CYLIVAASFFVHEDP 445

BLAST of MS004970 vs. TAIR 10
Match: AT3G51860.1 (cation exchanger 3 )

HSP 1 Score: 342.4 bits (877), Expect = 5.4e-94
Identity = 201/390 (51.54%), Postives = 269/390 (68.97%), Query Frame = 0

Query: 64  QKFCYGNMKNRIIDSIKIVVFSAKINLLMPFGPLAILVSSLSGHHGWVFLLSLLGIIPLA 123
           QK     +KN I+ +++ V+   K+ LL    PLAIL +S +     +F LSL+G+ PLA
Sbjct: 52  QKVPCKTLKN-ILSNLQEVILGTKLTLLFLAIPLAILANSYNYGRPLIFGLSLIGLTPLA 111

Query: 124 ERLGYATEQLACYTGATVGGLLNATFGNATELIISIYALRSGMIRVVQQSLLGSILSNML 183
           ER+ + TEQLA YTG TVGGLLNAT GNATELII+I AL +  + VV+ SLLGSILSN+L
Sbjct: 112 ERVSFLTEQLAFYTGPTVGGLLNATCGNATELIIAILALANNKVAVVKYSLLGSILSNLL 171

Query: 184 LVLGCAFFAGGVVVSKREQVFSKGSATVNSGLLLMAVMGLLFPAVLHSTHT----ELHFG 243
           LVLG + F GG+   +REQ F +  A VN  LLLM ++  L P +L    T         
Sbjct: 172 LVLGTSLFFGGIANIRREQRFDRKQADVNFFLLLMGLLCHLLPLLLKYAATGEVSTSMIN 231

Query: 244 KSELALSRFSSGIMLVAYAAYLVFQLKSEKNLYHPVDVVETEENSDD--------EESPE 303
           K  L LSR SS +ML+AY AYL+FQL + + L+      E +++ DD        EE+P 
Sbjct: 232 KMSLTLSRTSSIVMLIAYIAYLIFQLWTHRQLF------EAQQDDDDAYDDEVSVEETPV 291

Query: 304 ISMWESIIWLSILTIWISVLSEYLVNAIEGASVAMDIPVAFISVILLPIVGNAAEHAGAI 363
           I  W    WL  +TI I++LSEY+V+ IE AS +  + V+FIS+ILLPIVGNAAEHAGAI
Sbjct: 292 IGFWSGFAWLVGMTIVIALLSEYVVDTIEDASDSWGLSVSFISIILLPIVGNAAEHAGAI 351

Query: 364 MFAMKDKLDISLGVAIGSSTQISMFGIPFCVVIGWMMGVPMDLNFQLFETATLFITVIVV 423
           +FA K+KLDISLGVA+GS+TQIS+F +P  V++ W++G+ MDLNF + ET++L + +I+ 
Sbjct: 352 IFAFKNKLDISLGVALGSATQISLFVVPLSVIVAWILGIKMDLNFNILETSSLALAIIIT 411

Query: 424 AFMLQDGTSNYLKGLMLILCYLIVAASFFV 442
           AF LQDGTS+Y+KGL+L+LCY+I+AA FFV
Sbjct: 412 AFTLQDGTSHYMKGLVLLLCYVIIAACFFV 434

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140503.15.8e-23999.11vacuolar cation/proton exchanger 3-like [Momordica charantia] >XP_022140504.1 va... [more]
XP_008448138.11.0e-21991.31PREDICTED: vacuolar cation/proton exchanger 3-like isoform X1 [Cucumis melo][more]
KGN46624.16.2e-21790.65hypothetical protein Csa_005203 [Cucumis sativus][more]
NP_001267689.14.0e-21690.63vacuolar cation/proton exchanger 5-like [Cucumis sativus] >AFQ99298.1 cation exc... [more]
XP_011656306.24.0e-21690.63vacuolar cation/proton exchanger 5-like isoform X4 [Cucumis sativus] >XP_0317423... [more]
Match NameE-valueIdentityDescription
Q8L7836.2e-15978.93Vacuolar cation/proton exchanger 5 OS=Arabidopsis thaliana OX=3702 GN=CAX5 PE=2 ... [more]
Q392541.7e-15677.11Vacuolar cation/proton exchanger 2 OS=Arabidopsis thaliana OX=3702 GN=CAX2 PE=1 ... [more]
Q5KQN07.8e-15470.98Vacuolar cation/proton exchanger 2 OS=Oryza sativa subsp. japonica OX=39947 GN=C... [more]
Q6K1C41.6e-15175.27Vacuolar cation/proton exchanger 3 OS=Oryza sativa subsp. japonica OX=39947 GN=C... [more]
Q9LFZ83.5e-14674.40Putative vacuolar cation/proton exchanger 6 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Match NameE-valueIdentityDescription
A0A6J1CF972.8e-23999.11Vacuolar cation/proton exchanger OS=Momordica charantia OX=3673 GN=LOC111011152 ... [more]
A0A1S3BJ065.0e-22091.31Vacuolar cation/proton exchanger OS=Cucumis melo OX=3656 GN=LOC103490425 PE=3 SV... [more]
A0A0A0KCR23.0e-21790.65Vacuolar cation/proton exchanger OS=Cucumis sativus OX=3659 GN=Csa_6G115100 PE=3... [more]
J7KEC32.0e-21690.63Vacuolar cation/proton exchanger OS=Cucumis sativus OX=3659 GN=CAX6 PE=2 SV=1[more]
A0A6J1FW922.4e-21489.51Vacuolar cation/proton exchanger OS=Cucurbita moschata OX=3662 GN=LOC111449068 P... [more]
Match NameE-valueIdentityDescription
AT1G55730.14.4e-16078.93cation exchanger 5 [more]
AT1G55730.24.4e-16078.93cation exchanger 5 [more]
AT3G13320.11.2e-15777.11cation exchanger 2 [more]
AT1G55720.12.5e-14774.40cation exchanger 6 [more]
AT3G51860.15.4e-9451.54cation exchanger 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004798Calcium/proton exchanger CAX-likeTIGRFAMTIGR00378TIGR00378coord: 92..440
e-value: 2.1E-102
score: 340.9
NoneNo IPR availableTIGRFAMTIGR00846TIGR00846coord: 76..440
e-value: 8.8E-119
score: 394.5
NoneNo IPR availablePANTHERPTHR31503:SF67VACUOLAR CATION/PROTON EXCHANGERcoord: 14..451
IPR004837Sodium/calcium exchanger membrane regionPFAMPF01699Na_Ca_excoord: 298..437
e-value: 1.6E-18
score: 67.1
coord: 109..265
e-value: 3.8E-23
score: 82.1
IPR044880NCX, central ion-binding domain superfamilyGENE3D1.20.1420.30coord: 283..441
e-value: 2.7E-19
score: 71.4
coord: 138..280
e-value: 4.3E-16
score: 61.0
IPR004713Calcium/proton exchangerPANTHERPTHR31503VACUOLAR CALCIUM ION TRANSPORTERcoord: 14..451

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS004970.1MS004970.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006816 calcium ion transport
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0015369 calcium:proton antiporter activity
molecular_function GO:0008324 cation transmembrane transporter activity