MS004875 (gene) Bitter gourd (TR) v1

Overview
NameMS004875
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionAPO protein 4, mitochondrial
Locationscaffold176: 1138850 .. 1140287 (-)
RNA-Seq ExpressionMS004875
SyntenyMS004875
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTTTGTGGCTCTCAGAAGGAAATTGGTTCAGAACGTTGTGCAGGAATTCATGCTACACCGGTGCTATAGCTCTAAGGTCGACCTTAAGAAACTCCGTCCAATGATTTCGAAGAGAATTGAAAATCGGGCGAAATTCTATCCTATAAAGGCCATGATTCCAGTGGCACAACAAGTTCTTGAAGCTAGGGCAGTGCTTATTCATGGAGTTTCCACTCTTCTTAAGGCATTTCCAATTGTCTCTTGCAAGTCAGTGTCCGTTGAACAAACTCTCTTTAATTTCTCATCTTTGTGTCATGATTTTACTTTGTTTTCTTTTCTTTTTCCTATCTTTTTACATAGTTTTTAATCTAAATTGAATATTCCTTGAACTCATCTGTGTATTGGGGTAGTACCATTTTTGTCAGAAATTCTGAGTGACCTTGATTTTCTGTTTCCTGACTAGTAGCAGATATAGGAAATTTCCTGTACATGAAGTGAGGTCAATGAATACAATAATATATATATATATTATCTAGTTCTTTATACGCGCTAGGACCGTATGTATAATCTGTTCTTTTTAAAATTTTGAGGCGTTGATGTTAATTCGTGTTTGTGAATGCTGCTTCTAAAACTCCTAGACTCATTGTGTAAGTCTGTTCTTTCTAATTTTAGATTTTCGTTGTTAACTGAATACATGTTTGTGAATTGTGCTTCTGAAACTCCTAGGATCATTTTGTAGATTTTGTCCAGAAGTATATGTCGGTGAGCAAGGTCATCTAATACGGAGTTGTGGCGGTTACAAACGTGGTGCCAAGAATCGGGTTCATGAATGGATCGGAGGTGATTTGAATGATGTTCTTGTCCCAGTAGAAGCATTTCACCTCCATCGCATGTTCCAAGATGTTATTAAACATGATGAGAGATTCAACTTTGAACGTGTTCCAGCAGTTGCCGAGCTATGCTGGCAAGCTGGAGCCAATCCAAATGATGAAAATCTGTCTTCAAGCACTGGGAATTCAGATGGTGGTGGCTCAGGCAGAGATGAATGGTTGTCAAGTCATGAGCTGATGCTTTTAGCAAATGTAACTCTCAAGGCATGGGAAACGCTTAGAACCGGTGTGCAGAAACTGTTAATGGTTTATCCTGCTAAGGTCTGTAAATACTGCTCAGAAGTTCATGTTGGGCCATCAGGACACAAAGCTAGACTTTGTGGGGTTTTTAAATATGAGAGTTGGCGAGGCTCCCACTTCTGGGAGAAGGCAAATGTAGACGATTTAGTTCCTCCAAATATTGTATGGCATCGGCGACCACAAGATCCTCCCATGCTTGTCGACGAAGGAAGGGATTATTATGGACATGTACCAGCAGTGGTGGCTCTTTGCACACAGGCCGGCGTCGTTGCACCATCTAAATATCATTGCATGATGAAAATTCAAGGCTTATCACCACGAACT

mRNA sequence

ATGGCTTTTGTGGCTCTCAGAAGGAAATTGGTTCAGAACGTTGTGCAGGAATTCATGCTACACCGGTGCTATAGCTCTAAGGTCGACCTTAAGAAACTCCGTCCAATGATTTCGAAGAGAATTGAAAATCGGGCGAAATTCTATCCTATAAAGGCCATGATTCCAGTGGCACAACAAGTTCTTGAAGCTAGGGCAGTGCTTATTCATGGAGTTTCCACTCTTCTTAAGGCATTTCCAATTGTCTCTTGCAAATTTTGTCCAGAAGTATATGTCGGTGAGCAAGGTCATCTAATACGGAGTTGTGGCGGTTACAAACGTGGTGCCAAGAATCGGGTTCATGAATGGATCGGAGGTGATTTGAATGATGTTCTTGTCCCAGTAGAAGCATTTCACCTCCATCGCATGTTCCAAGATGTTATTAAACATGATGAGAGATTCAACTTTGAACGTGTTCCAGCAGTTGCCGAGCTATGCTGGCAAGCTGGAGCCAATCCAAATGATGAAAATCTGTCTTCAAGCACTGGGAATTCAGATGGTGGTGGCTCAGGCAGAGATGAATGGTTGTCAAGTCATGAGCTGATGCTTTTAGCAAATGTAACTCTCAAGGCATGGGAAACGCTTAGAACCGGTGTGCAGAAACTGTTAATGGTTTATCCTGCTAAGGTCTGTAAATACTGCTCAGAAGTTCATGTTGGGCCATCAGGACACAAAGCTAGACTTTGTGGGGTTTTTAAATATGAGAGTTGGCGAGGCTCCCACTTCTGGGAGAAGGCAAATGTAGACGATTTAGTTCCTCCAAATATTGTATGGCATCGGCGACCACAAGATCCTCCCATGCTTGTCGACGAAGGAAGGGATTATTATGGACATGTACCAGCAGTGGTGGCTCTTTGCACACAGGCCGGCGTCGTTGCACCATCTAAATATCATTGCATGATGAAAATTCAAGGCTTATCACCACGAACT

Coding sequence (CDS)

ATGGCTTTTGTGGCTCTCAGAAGGAAATTGGTTCAGAACGTTGTGCAGGAATTCATGCTACACCGGTGCTATAGCTCTAAGGTCGACCTTAAGAAACTCCGTCCAATGATTTCGAAGAGAATTGAAAATCGGGCGAAATTCTATCCTATAAAGGCCATGATTCCAGTGGCACAACAAGTTCTTGAAGCTAGGGCAGTGCTTATTCATGGAGTTTCCACTCTTCTTAAGGCATTTCCAATTGTCTCTTGCAAATTTTGTCCAGAAGTATATGTCGGTGAGCAAGGTCATCTAATACGGAGTTGTGGCGGTTACAAACGTGGTGCCAAGAATCGGGTTCATGAATGGATCGGAGGTGATTTGAATGATGTTCTTGTCCCAGTAGAAGCATTTCACCTCCATCGCATGTTCCAAGATGTTATTAAACATGATGAGAGATTCAACTTTGAACGTGTTCCAGCAGTTGCCGAGCTATGCTGGCAAGCTGGAGCCAATCCAAATGATGAAAATCTGTCTTCAAGCACTGGGAATTCAGATGGTGGTGGCTCAGGCAGAGATGAATGGTTGTCAAGTCATGAGCTGATGCTTTTAGCAAATGTAACTCTCAAGGCATGGGAAACGCTTAGAACCGGTGTGCAGAAACTGTTAATGGTTTATCCTGCTAAGGTCTGTAAATACTGCTCAGAAGTTCATGTTGGGCCATCAGGACACAAAGCTAGACTTTGTGGGGTTTTTAAATATGAGAGTTGGCGAGGCTCCCACTTCTGGGAGAAGGCAAATGTAGACGATTTAGTTCCTCCAAATATTGTATGGCATCGGCGACCACAAGATCCTCCCATGCTTGTCGACGAAGGAAGGGATTATTATGGACATGTACCAGCAGTGGTGGCTCTTTGCACACAGGCCGGCGTCGTTGCACCATCTAAATATCATTGCATGATGAAAATTCAAGGCTTATCACCACGAACT

Protein sequence

MAFVALRRKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPIKAMIPVAQQVLEARAVLIHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDLNDVLVPVEAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNSDGGGSGRDEWLSSHELMLLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYESWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQAGVVAPSKYHCMMKIQGLSPRT
Homology
BLAST of MS004875 vs. NCBI nr
Match: XP_022140768.1 (APO protein 4, mitochondrial [Momordica charantia])

HSP 1 Score: 655.6 bits (1690), Expect = 2.2e-184
Identity = 313/315 (99.37%), Postives = 313/315 (99.37%), Query Frame = 0

Query: 8   RKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPIKAMIPVAQQVLEARAVL 67
           RKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPI AMIPVAQQVLEARAVL
Sbjct: 22  RKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPINAMIPVAQQVLEARAVL 81

Query: 68  IHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDLNDVLVPV 127
           IHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDLNDVLVPV
Sbjct: 82  IHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDLNDVLVPV 141

Query: 128 EAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNSDGGGSGRDEW 187
           EAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNSDGGGSGRDEW
Sbjct: 142 EAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNSDGGGSGRDEW 201

Query: 188 LSSHELMLLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYE 247
           LSSHELM LANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYE
Sbjct: 202 LSSHELMPLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYE 261

Query: 248 SWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQAGVVAPS 307
           SWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQAGVVAPS
Sbjct: 262 SWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQAGVVAPS 321

Query: 308 KYHCMMKIQGLSPRT 323
           KYHCMMKIQGLSPRT
Sbjct: 322 KYHCMMKIQGLSPRT 336

BLAST of MS004875 vs. NCBI nr
Match: XP_038901944.1 (APO protein 4, mitochondrial [Benincasa hispida] >XP_038901945.1 APO protein 4, mitochondrial [Benincasa hispida] >XP_038901946.1 APO protein 4, mitochondrial [Benincasa hispida] >XP_038901947.1 APO protein 4, mitochondrial [Benincasa hispida])

HSP 1 Score: 581.6 bits (1498), Expect = 4.1e-162
Identity = 269/323 (83.28%), Postives = 300/323 (92.88%), Query Frame = 0

Query: 1   MAFVALRRKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPIKAMIPVAQQV 60
           MAF+AL+RK  + VVQEF+LHRCYSSKV+LKKLRPMI KRI+NR K YPI+ M PVAQQV
Sbjct: 4   MAFMALKRKFREIVVQEFVLHRCYSSKVNLKKLRPMILKRIQNREKNYPIRGMTPVAQQV 63

Query: 61  LEARAVLIHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDL 120
           LEAR +LIHGVSTLLK+FP++SCKFCPEVYVGE+GHLIRSCGGYKRGAKN+VHEWI GDL
Sbjct: 64  LEARTMLIHGVSTLLKSFPVLSCKFCPEVYVGEKGHLIRSCGGYKRGAKNQVHEWIRGDL 123

Query: 121 NDVLVPVEAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNS-DG 180
           ND++VPVE+FHLH MFQDVIKHDERFNFERVPAV ELCWQAGANPNDEN SSST NS +G
Sbjct: 124 NDIIVPVESFHLHHMFQDVIKHDERFNFERVPAVVELCWQAGANPNDENQSSSTRNSGEG 183

Query: 181 GGSGRDEWLSSHELMLLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKAR 240
           GGSGRD+ LS HE+MLLA  T++AWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKAR
Sbjct: 184 GGSGRDKPLSGHEMMLLATETIRAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKAR 243

Query: 241 LCGVFKYESWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCT 300
           LCGVFKYE WRGSHFWEKA++DDLVPP +VWHRRPQDPP+L+DEGRDYYGH PAV+ALCT
Sbjct: 244 LCGVFKYEHWRGSHFWEKADIDDLVPPKLVWHRRPQDPPVLIDEGRDYYGHTPAVLALCT 303

Query: 301 QAGVVAPSKYHCMMKIQGLSPRT 323
           QAG +APSKYHCMMK+QG+SPR+
Sbjct: 304 QAGALAPSKYHCMMKVQGISPRS 326

BLAST of MS004875 vs. NCBI nr
Match: XP_023532078.1 (APO protein 4, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 576.2 bits (1484), Expect = 1.7e-160
Identity = 271/321 (84.42%), Postives = 295/321 (91.90%), Query Frame = 0

Query: 1   MAFVALRRKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPIKAMIPVAQQV 60
           MAF+ALRRKL +NVVQEFML RCYSSKVDLKKLRPMI KR++NRA  YPIK MIPVAQ+V
Sbjct: 1   MAFMALRRKLGENVVQEFMLQRCYSSKVDLKKLRPMILKRLQNRANNYPIKGMIPVAQEV 60

Query: 61  LEARAVLIHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDL 120
           LEARA LIHGVSTLLK FP++SCKFCPEVYVG++GHLIRSCGGYK GAKNRVHEWI GDL
Sbjct: 61  LEARAKLIHGVSTLLKTFPVLSCKFCPEVYVGDKGHLIRSCGGYKHGAKNRVHEWIRGDL 120

Query: 121 NDVLVPVEAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNS--D 180
           ND+LVPVE+FHL  MFQDVIKHDERF+F+RVPAV ELCWQAGANPNDENLSSST NS  D
Sbjct: 121 NDILVPVESFHLRHMFQDVIKHDERFDFDRVPAVVELCWQAGANPNDENLSSSTWNSDGD 180

Query: 181 GGGSGRDEWLSSHELMLLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKA 240
           G G+GRDE LSSHELM+LAN T++AWET+RTGVQKLLMVYP KVCK+CSEVHVGPSGHKA
Sbjct: 181 GNGNGRDEHLSSHELMVLANTTIRAWETVRTGVQKLLMVYPTKVCKHCSEVHVGPSGHKA 240

Query: 241 RLCGVFKYESWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALC 300
           RLCGVFKYESWRGSHFWEKA+VDDLVPP  VWHRR QDPP+LVDEGRDYYGH PAV+ALC
Sbjct: 241 RLCGVFKYESWRGSHFWEKADVDDLVPPKSVWHRRAQDPPVLVDEGRDYYGHAPAVLALC 300

Query: 301 TQAGVVAPSKYHCMMKIQGLS 320
            QAG +APSKYHCMMK+QGLS
Sbjct: 301 LQAGAIAPSKYHCMMKVQGLS 321

BLAST of MS004875 vs. NCBI nr
Match: XP_022971049.1 (APO protein 4, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 575.9 bits (1483), Expect = 2.2e-160
Identity = 271/319 (84.95%), Postives = 294/319 (92.16%), Query Frame = 0

Query: 1   MAFVALRRKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPIKAMIPVAQQV 60
           MAF+ALRRKL +NVVQEFML RCYSSKVDLKKLRPMI KR++NRA  YPIK MIPVAQ+V
Sbjct: 1   MAFMALRRKLGENVVQEFMLQRCYSSKVDLKKLRPMILKRLQNRANNYPIKGMIPVAQEV 60

Query: 61  LEARAVLIHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDL 120
           LEARA+LIHGVSTLLK FP++SCKFCPEVYVG++GHLIRSCGGYKRGAKNRVHEWI GDL
Sbjct: 61  LEARAMLIHGVSTLLKTFPVLSCKFCPEVYVGDKGHLIRSCGGYKRGAKNRVHEWIRGDL 120

Query: 121 NDVLVPVEAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNSDGG 180
           ND+LVPVE+FHL  MFQDVIKHDERF+FERVPAV ELCWQAGANPNDENLSSST NSD  
Sbjct: 121 NDILVPVESFHLRHMFQDVIKHDERFDFERVPAVVELCWQAGANPNDENLSSSTWNSD-- 180

Query: 181 GSGRDEWLSSHELMLLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARL 240
           G GRDE LSSHELM+LAN T++AWET+RTGVQKLLMVYP KVCK+CSEVHVGPSGHKARL
Sbjct: 181 GDGRDEHLSSHELMVLANSTIRAWETVRTGVQKLLMVYPTKVCKHCSEVHVGPSGHKARL 240

Query: 241 CGVFKYESWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQ 300
           CGVFKYESWRGSHFWEK +VDDLVPP  VWHRR QDPP+LVDEGRDYYGH PAV+ALC Q
Sbjct: 241 CGVFKYESWRGSHFWEKGDVDDLVPPKSVWHRRAQDPPVLVDEGRDYYGHAPAVLALCIQ 300

Query: 301 AGVVAPSKYHCMMKIQGLS 320
           AG +APSKYHCMMK+QGLS
Sbjct: 301 AGAIAPSKYHCMMKVQGLS 317

BLAST of MS004875 vs. NCBI nr
Match: XP_004151879.1 (APO protein 4, mitochondrial [Cucumis sativus] >XP_031737269.1 APO protein 4, mitochondrial [Cucumis sativus] >XP_031737270.1 APO protein 4, mitochondrial [Cucumis sativus] >KGN63269.1 hypothetical protein Csa_022178 [Cucumis sativus])

HSP 1 Score: 570.9 bits (1470), Expect = 7.2e-159
Identity = 269/322 (83.54%), Postives = 296/322 (91.93%), Query Frame = 0

Query: 1   MAFVALRRKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPIKAMIPVAQQV 60
           MAF+A+RRK   NVVQEFML RCYSSKV+LKKLRPMI KRI++RAK YPIK M PVAQQV
Sbjct: 4   MAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVAQQV 63

Query: 61  LEARAVLIHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDL 120
           LEARA+LIHGVSTLLK+FP++SCKFCPEVYVGE+GHLIRSCGGYKRGAKN+VH+WI GDL
Sbjct: 64  LEARAMLIHGVSTLLKSFPVLSCKFCPEVYVGEEGHLIRSCGGYKRGAKNQVHQWIRGDL 123

Query: 121 NDVLVPVEAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNS-DG 180
            D++VPVEAFHLH MFQDVIKHDERFNFERVPAV ELC QAGANP+D+NL+SST NS +G
Sbjct: 124 KDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCSQAGANPDDKNLASSTQNSAEG 183

Query: 181 GGSGRDEWLSSHELMLLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKAR 240
           GGSG DE LS HE+MLLA  T++AWETLRTGVQKLLMVYP KVCKYCSEVHVGPSGHKAR
Sbjct: 184 GGSGMDEPLSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGHKAR 243

Query: 241 LCGVFKYESWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCT 300
           LCGVF YESWRGSHFWEKA+VDDLVPP IVWHRR QDPP+LVD+G+DYYGH PAVVALCT
Sbjct: 244 LCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYYGHAPAVVALCT 303

Query: 301 QAGVVAPSKYHCMMKIQGLSPR 322
           QAGV+AP KYHCMMK+QGLSPR
Sbjct: 304 QAGVIAPFKYHCMMKVQGLSPR 325

BLAST of MS004875 vs. ExPASy Swiss-Prot
Match: Q9LSZ0 (APO protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=APO4 PE=2 SV=2)

HSP 1 Score: 377.5 bits (968), Expect = 1.5e-103
Identity = 175/292 (59.93%), Postives = 222/292 (76.03%), Query Frame = 0

Query: 29  DLKKLRPMISKRIENRAKFYPIKAMIPVAQQVLEARAVLIHGVSTLLKAFPIVSCKFCPE 88
           DL+KLRPMI KRIENRAK YP+K ++PVA+++L AR  LI  ++ LLK FP+++CKFC E
Sbjct: 34  DLRKLRPMILKRIENRAKDYPVKEIVPVAEEILIARKNLISNIAALLKVFPVLTCKFCSE 93

Query: 89  VYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDLNDVLVPVEAFHLHRMFQDVIKHDERFNF 148
           V+VG++GHLI +C  Y R   NR+HEW+ G +ND+LVPVE++HLH + Q VI+H ERF++
Sbjct: 94  VFVGKEGHLIETCRSYIRRGNNRLHEWVPGSINDILVPVESYHLHNISQGVIRHQERFDY 153

Query: 149 ERVPAVAELCWQAGANPNDENLSSSTGNSDGGGSGRD-EWLSSHELMLLANVTLKAWETL 208
           +RVPA+ ELC QAGA   +E L  S  + +   S  D   L + +L  +    L AWE +
Sbjct: 154 DRVPAILELCCQAGAIHPEEILQYSEIHDNPQISEEDIRSLPAGDLKYVGANALMAWEKV 213

Query: 209 RTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYESWRGSHFWEKANVDDLVPPN 268
           R GV+KLL+VYP+KVCK C EVHVGPSGHKARLCGVFKYESWRG+H+WEKA V+DLVP  
Sbjct: 214 RAGVKKLLLVYPSKVCKRCKEVHVGPSGHKARLCGVFKYESWRGTHYWEKAGVNDLVPEK 273

Query: 269 IVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQAGVVAPSKYHCMMKIQGLS 320
           +VWHRRPQDP +LVDEGR YYGH PA+V+LC+  G + P KY C MK QGLS
Sbjct: 274 MVWHRRPQDPVVLVDEGRSYYGHAPAIVSLCSHTGAIVPVKYACKMKPQGLS 325

BLAST of MS004875 vs. ExPASy Swiss-Prot
Match: Q8W4A5 (APO protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APO2 PE=2 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 2.6e-55
Identity = 111/297 (37.37%), Postives = 157/297 (52.86%), Query Frame = 0

Query: 50  IKAMIPVAQQVLEARAVLIHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAK 109
           +K+++P+A +V  AR  LI+ +  L+K   + +C +C E++VG  GH  +SC G     +
Sbjct: 130 VKSLVPLAYKVYNARIRLINNLHRLMKVVRVNACGWCNEIHVGPYGHPFKSCKGPNTSQR 189

Query: 110 NRVHEWIGGDLNDVLVPVEAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGAN----- 169
             +HEW    + DV+VP+EA+HL       I+HDERF+  RVPAV ELC Q G       
Sbjct: 190 KGLHEWTNSVIEDVIVPLEAYHLFDRLGKRIRHDERFSIPRVPAVVELCIQGGVEIPEFP 249

Query: 170 -PNDENLSSSTGNSDGGGSGRDEW------------------------LSSHELMLLANV 229
                      G S+   +   E                          S  E + LA  
Sbjct: 250 AKRRRKPIIRIGKSEFVDADETELPDPEPQPPPVPLLTELPVSEITPPSSEEETVSLAEE 309

Query: 230 TLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYESWRGSHFWEKAN 289
           TL+AWE +R G +KL+ +Y  +VC YC EVHVGP+GHKA+ CG FK++   G H W+ A 
Sbjct: 310 TLQAWEEMRAGAKKLMRMYRVRVCGYCPEVHVGPTGHKAQNCGAFKHQQRNGQHGWQSAV 369

Query: 290 VDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQAGVVAPSKYHCMMKIQ 317
           +DDL+PP  VWH    + P +  E R +YG  PAVV +C QAG V P  Y   M+++
Sbjct: 370 LDDLIPPRYVWHVPDVNGPPMQRELRSFYGQAPAVVEICAQAGAVVPEHYRATMRLE 426

BLAST of MS004875 vs. ExPASy Swiss-Prot
Match: Q9XIR4 (APO protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APO1 PE=2 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 6.1e-52
Identity = 114/325 (35.08%), Postives = 164/325 (50.46%), Query Frame = 0

Query: 31  KKLRPM-ISKRIENRAKFYPIKAMIPVAQQVLEARAVLIHGVSTLLKAFPIVSCKFCPEV 90
           KKL  M I K+++       +  ++PVA QV++   +LI G++ LL   P+ +C  C  V
Sbjct: 103 KKLAQMGIEKQLDPPKNGLLVPNLVPVADQVIDNWKLLIKGLAQLLHVVPVFACSECGAV 162

Query: 91  YVGEQGHLIRSCGGYKRGAKNRVHEWIGGDLNDVLVPVEAFHLHRMFQDVIKHDERFNFE 150
           +V   GH IR C G     +   H W+ G +NDVL+PVE++H++  F   IKH+ RF +E
Sbjct: 163 HVANVGHNIRDCNGPTNSQRRGSHSWVKGTINDVLIPVESYHMYDPFGRRIKHETRFEYE 222

Query: 151 RVPAVAELCWQAGAN---------------------------------PNDENLSSSTGN 210
           R+PA+ ELC QAG                                       +LSS    
Sbjct: 223 RIPALVELCIQAGVEIPEYPCRRRTQPIRMMGKRVIDRGGYHKEPEKPQTSSSLSSPLAE 282

Query: 211 SDG-GGSGRDEWLSSHELMLLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSG 270
            D  G   R    +  ++  +A  T+ A+E +R GV KL+  +  K C YCSEVHVGP G
Sbjct: 283 LDTLGVFERYPPPTPEDIPKIAQETMDAYEKVRLGVTKLMRKFTVKACGYCSEVHVGPWG 342

Query: 271 HKARLCGVFKYESWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVV 321
           H  +LCG FK++   G H W+ A VD++ PPN VWH R      L    R +YG  PA+V
Sbjct: 343 HSVKLCGEFKHQWRDGKHGWQDALVDEVFPPNYVWHVRDLKGNPLTGNLRRFYGKAPALV 402

BLAST of MS004875 vs. ExPASy Swiss-Prot
Match: Q9FH50 (APO protein 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=APO3 PE=2 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 3.6e-44
Identity = 100/303 (33.00%), Postives = 159/303 (52.48%), Query Frame = 0

Query: 50  IKAMIPVAQQVLEARAVLIHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAK 109
           +  ++ VA  V   R +L+ G+S ++   P+  C+ C EV++G+QGH IR+C G   G++
Sbjct: 95  VPELVDVAHCVHRCRNMLLSGLSKIIHHVPVHRCRLCAEVHIGKQGHEIRTCTGPGSGSR 154

Query: 110 NRVHEWIGGDLNDVLVPVEAFHLH-RMFQDVIKHDERFNFERVPAVAELCWQAGA----- 169
           +  H W  G ++DV++  + FHL+ R  +  + HDERF   ++ AV ELC QAG      
Sbjct: 155 SATHVWKRGRVSDVVLFPKCFHLYDRAVKPRVIHDERFTVPKISAVLELCIQAGVDLEKF 214

Query: 170 --------------------NPNDEN----LSSSTGNSDGGGSGRDEWLSSHELMLLANV 229
                               + ND N    ++S+T         ++E  S  EL      
Sbjct: 215 PSKRRSKPVYSIEGRIVDFEDVNDGNSELAVTSTTTLIQEDDRCKEEKKSLKELSF---E 274

Query: 230 TLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYESWRGSHFWEKAN 289
           T+++W  +  GV+KL+  Y    C YC E+ VGP GHK R+C   K++   G H W++A 
Sbjct: 275 TMESWFEMVLGVRKLMERYRVWTCGYCPEIQVGPKGHKVRMCKATKHQMRDGMHAWQEAT 334

Query: 290 VDDLVPPNIVWH-RRPQDPPMLVDEGRDYYGHVPAVVALCTQAGVVAPSKYHCMMKIQGL 322
           +DD+V P  VWH R P D  +L +  + +YG  PAV+ +C Q G   P +Y+ MM++  +
Sbjct: 335 IDDVVGPTYVWHVRDPTDGSVLDNSLKRFYGKAPAVIEMCVQGGAPVPDQYNSMMRLDVV 394

BLAST of MS004875 vs. ExPASy TrEMBL
Match: A0A6J1CIR9 (APO protein 4, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111011348 PE=4 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 1.1e-184
Identity = 313/315 (99.37%), Postives = 313/315 (99.37%), Query Frame = 0

Query: 8   RKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPIKAMIPVAQQVLEARAVL 67
           RKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPI AMIPVAQQVLEARAVL
Sbjct: 22  RKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPINAMIPVAQQVLEARAVL 81

Query: 68  IHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDLNDVLVPV 127
           IHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDLNDVLVPV
Sbjct: 82  IHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDLNDVLVPV 141

Query: 128 EAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNSDGGGSGRDEW 187
           EAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNSDGGGSGRDEW
Sbjct: 142 EAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNSDGGGSGRDEW 201

Query: 188 LSSHELMLLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYE 247
           LSSHELM LANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYE
Sbjct: 202 LSSHELMPLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYE 261

Query: 248 SWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQAGVVAPS 307
           SWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQAGVVAPS
Sbjct: 262 SWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQAGVVAPS 321

Query: 308 KYHCMMKIQGLSPRT 323
           KYHCMMKIQGLSPRT
Sbjct: 322 KYHCMMKIQGLSPRT 336

BLAST of MS004875 vs. ExPASy TrEMBL
Match: A0A6J1I7G7 (APO protein 4, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111469835 PE=4 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 1.1e-160
Identity = 271/319 (84.95%), Postives = 294/319 (92.16%), Query Frame = 0

Query: 1   MAFVALRRKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPIKAMIPVAQQV 60
           MAF+ALRRKL +NVVQEFML RCYSSKVDLKKLRPMI KR++NRA  YPIK MIPVAQ+V
Sbjct: 1   MAFMALRRKLGENVVQEFMLQRCYSSKVDLKKLRPMILKRLQNRANNYPIKGMIPVAQEV 60

Query: 61  LEARAVLIHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDL 120
           LEARA+LIHGVSTLLK FP++SCKFCPEVYVG++GHLIRSCGGYKRGAKNRVHEWI GDL
Sbjct: 61  LEARAMLIHGVSTLLKTFPVLSCKFCPEVYVGDKGHLIRSCGGYKRGAKNRVHEWIRGDL 120

Query: 121 NDVLVPVEAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNSDGG 180
           ND+LVPVE+FHL  MFQDVIKHDERF+FERVPAV ELCWQAGANPNDENLSSST NSD  
Sbjct: 121 NDILVPVESFHLRHMFQDVIKHDERFDFERVPAVVELCWQAGANPNDENLSSSTWNSD-- 180

Query: 181 GSGRDEWLSSHELMLLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARL 240
           G GRDE LSSHELM+LAN T++AWET+RTGVQKLLMVYP KVCK+CSEVHVGPSGHKARL
Sbjct: 181 GDGRDEHLSSHELMVLANSTIRAWETVRTGVQKLLMVYPTKVCKHCSEVHVGPSGHKARL 240

Query: 241 CGVFKYESWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQ 300
           CGVFKYESWRGSHFWEK +VDDLVPP  VWHRR QDPP+LVDEGRDYYGH PAV+ALC Q
Sbjct: 241 CGVFKYESWRGSHFWEKGDVDDLVPPKSVWHRRAQDPPVLVDEGRDYYGHAPAVLALCIQ 300

Query: 301 AGVVAPSKYHCMMKIQGLS 320
           AG +APSKYHCMMK+QGLS
Sbjct: 301 AGAIAPSKYHCMMKVQGLS 317

BLAST of MS004875 vs. ExPASy TrEMBL
Match: A0A0A0LQG5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G421010 PE=4 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 3.5e-159
Identity = 269/322 (83.54%), Postives = 296/322 (91.93%), Query Frame = 0

Query: 1   MAFVALRRKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPIKAMIPVAQQV 60
           MAF+A+RRK   NVVQEFML RCYSSKV+LKKLRPMI KRI++RAK YPIK M PVAQQV
Sbjct: 4   MAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVAQQV 63

Query: 61  LEARAVLIHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDL 120
           LEARA+LIHGVSTLLK+FP++SCKFCPEVYVGE+GHLIRSCGGYKRGAKN+VH+WI GDL
Sbjct: 64  LEARAMLIHGVSTLLKSFPVLSCKFCPEVYVGEEGHLIRSCGGYKRGAKNQVHQWIRGDL 123

Query: 121 NDVLVPVEAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNS-DG 180
            D++VPVEAFHLH MFQDVIKHDERFNFERVPAV ELC QAGANP+D+NL+SST NS +G
Sbjct: 124 KDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCSQAGANPDDKNLASSTQNSAEG 183

Query: 181 GGSGRDEWLSSHELMLLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKAR 240
           GGSG DE LS HE+MLLA  T++AWETLRTGVQKLLMVYP KVCKYCSEVHVGPSGHKAR
Sbjct: 184 GGSGMDEPLSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGHKAR 243

Query: 241 LCGVFKYESWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCT 300
           LCGVF YESWRGSHFWEKA+VDDLVPP IVWHRR QDPP+LVD+G+DYYGH PAVVALCT
Sbjct: 244 LCGVFTYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGKDYYGHAPAVVALCT 303

Query: 301 QAGVVAPSKYHCMMKIQGLSPR 322
           QAGV+AP KYHCMMK+QGLSPR
Sbjct: 304 QAGVIAPFKYHCMMKVQGLSPR 325

BLAST of MS004875 vs. ExPASy TrEMBL
Match: A0A6J1G8H9 (APO protein 4, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111451698 PE=4 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 3.5e-159
Identity = 267/319 (83.70%), Postives = 293/319 (91.85%), Query Frame = 0

Query: 1   MAFVALRRKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPIKAMIPVAQQV 60
           MAF+ALRRKL +NVVQEFML RCYSSKVDLKKLRPMI KR++NRA  YPIK MIPVAQ+V
Sbjct: 1   MAFMALRRKLGENVVQEFMLQRCYSSKVDLKKLRPMILKRLQNRANNYPIKGMIPVAQEV 60

Query: 61  LEARAVLIHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDL 120
           LEARA+LIHGVSTLLK FP++SCKFCPEVYVG++GHLIRSCGGYK GAKNRVHEW  GDL
Sbjct: 61  LEARAMLIHGVSTLLKTFPVLSCKFCPEVYVGDKGHLIRSCGGYKHGAKNRVHEWFRGDL 120

Query: 121 NDVLVPVEAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNSDGG 180
           ND+LVPVE+FHLH MFQDVIKHDERF+FERVPAV ELCWQAGANPNDE+LSS T NSD  
Sbjct: 121 NDILVPVESFHLHHMFQDVIKHDERFDFERVPAVVELCWQAGANPNDEHLSSGTWNSD-- 180

Query: 181 GSGRDEWLSSHELMLLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARL 240
           G+GRDE LSSHELM+LA+ T++AWET+RTGV KLLMVYP KVCK+CSEVHVGPSGHKARL
Sbjct: 181 GNGRDEHLSSHELMVLASTTIRAWETVRTGVHKLLMVYPTKVCKHCSEVHVGPSGHKARL 240

Query: 241 CGVFKYESWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQ 300
           CGVFKYESWRGSHFWEKA+VDDLVPP  VWHRR QDPP+LVDEGRDYYGH PAV+ALC Q
Sbjct: 241 CGVFKYESWRGSHFWEKADVDDLVPPKSVWHRRAQDPPVLVDEGRDYYGHAPAVLALCLQ 300

Query: 301 AGVVAPSKYHCMMKIQGLS 320
           AG +APSKYHCMMK+QGLS
Sbjct: 301 AGAIAPSKYHCMMKVQGLS 317

BLAST of MS004875 vs. ExPASy TrEMBL
Match: A0A5A7TJ18 (APO protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1639G00180 PE=4 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 5.0e-158
Identity = 266/322 (82.61%), Postives = 294/322 (91.30%), Query Frame = 0

Query: 1   MAFVALRRKLVQNVVQEFMLHRCYSSKVDLKKLRPMISKRIENRAKFYPIKAMIPVAQQV 60
           MAF+A+RRK   NVVQEFML RCYSSKV+LKKLRPMI KRI++RAK YPIK M PVAQQV
Sbjct: 4   MAFMAIRRKFRDNVVQEFMLQRCYSSKVNLKKLRPMILKRIQDRAKNYPIKGMTPVAQQV 63

Query: 61  LEARAVLIHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDL 120
           LEARA+LIHGVSTLLK+FP++SCK+CPEVYVG +GHLIRSCGGYKRGAKN+VH+WI GDL
Sbjct: 64  LEARAMLIHGVSTLLKSFPVLSCKYCPEVYVGAEGHLIRSCGGYKRGAKNQVHQWIRGDL 123

Query: 121 NDVLVPVEAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGANPNDENLSSSTGNS-DG 180
           ND++VPVEAFHLH MFQDVIKHDERFNFERVPAV ELC QAG NP+D++L+SST NS +G
Sbjct: 124 NDIIVPVEAFHLHHMFQDVIKHDERFNFERVPAVVELCCQAGVNPDDKDLASSTQNSAEG 183

Query: 181 GGSGRDEWLSSHELMLLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKAR 240
           GGSG DE  S HE+MLLA  T++AWETLRTGVQKLLMVYP KVCKYCSEVHVGPSGHKAR
Sbjct: 184 GGSGMDEPFSDHEMMLLATETIRAWETLRTGVQKLLMVYPTKVCKYCSEVHVGPSGHKAR 243

Query: 241 LCGVFKYESWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCT 300
           LCGVFKYESWRGSHFWEKA+VDDLVPP IVWHRR QDPP+LVD+GRDYYGH PAVVALCT
Sbjct: 244 LCGVFKYESWRGSHFWEKADVDDLVPPKIVWHRRQQDPPVLVDKGRDYYGHAPAVVALCT 303

Query: 301 QAGVVAPSKYHCMMKIQGLSPR 322
           QAG +AP KYHCMMK+QGLSPR
Sbjct: 304 QAGAIAPFKYHCMMKVQGLSPR 325

BLAST of MS004875 vs. TAIR 10
Match: AT3G21740.1 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 377.5 bits (968), Expect = 1.1e-104
Identity = 175/292 (59.93%), Postives = 222/292 (76.03%), Query Frame = 0

Query: 29  DLKKLRPMISKRIENRAKFYPIKAMIPVAQQVLEARAVLIHGVSTLLKAFPIVSCKFCPE 88
           DL+KLRPMI KRIENRAK YP+K ++PVA+++L AR  LI  ++ LLK FP+++CKFC E
Sbjct: 34  DLRKLRPMILKRIENRAKDYPVKEIVPVAEEILIARKNLISNIAALLKVFPVLTCKFCSE 93

Query: 89  VYVGEQGHLIRSCGGYKRGAKNRVHEWIGGDLNDVLVPVEAFHLHRMFQDVIKHDERFNF 148
           V+VG++GHLI +C  Y R   NR+HEW+ G +ND+LVPVE++HLH + Q VI+H ERF++
Sbjct: 94  VFVGKEGHLIETCRSYIRRGNNRLHEWVPGSINDILVPVESYHLHNISQGVIRHQERFDY 153

Query: 149 ERVPAVAELCWQAGANPNDENLSSSTGNSDGGGSGRD-EWLSSHELMLLANVTLKAWETL 208
           +RVPA+ ELC QAGA   +E L  S  + +   S  D   L + +L  +    L AWE +
Sbjct: 154 DRVPAILELCCQAGAIHPEEILQYSEIHDNPQISEEDIRSLPAGDLKYVGANALMAWEKV 213

Query: 209 RTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYESWRGSHFWEKANVDDLVPPN 268
           R GV+KLL+VYP+KVCK C EVHVGPSGHKARLCGVFKYESWRG+H+WEKA V+DLVP  
Sbjct: 214 RAGVKKLLLVYPSKVCKRCKEVHVGPSGHKARLCGVFKYESWRGTHYWEKAGVNDLVPEK 273

Query: 269 IVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQAGVVAPSKYHCMMKIQGLS 320
           +VWHRRPQDP +LVDEGR YYGH PA+V+LC+  G + P KY C MK QGLS
Sbjct: 274 MVWHRRPQDPVVLVDEGRSYYGHAPAIVSLCSHTGAIVPVKYACKMKPQGLS 325

BLAST of MS004875 vs. TAIR 10
Match: AT5G57930.1 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 217.2 bits (552), Expect = 1.9e-56
Identity = 111/297 (37.37%), Postives = 157/297 (52.86%), Query Frame = 0

Query: 50  IKAMIPVAQQVLEARAVLIHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAK 109
           +K+++P+A +V  AR  LI+ +  L+K   + +C +C E++VG  GH  +SC G     +
Sbjct: 130 VKSLVPLAYKVYNARIRLINNLHRLMKVVRVNACGWCNEIHVGPYGHPFKSCKGPNTSQR 189

Query: 110 NRVHEWIGGDLNDVLVPVEAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGAN----- 169
             +HEW    + DV+VP+EA+HL       I+HDERF+  RVPAV ELC Q G       
Sbjct: 190 KGLHEWTNSVIEDVIVPLEAYHLFDRLGKRIRHDERFSIPRVPAVVELCIQGGVEIPEFP 249

Query: 170 -PNDENLSSSTGNSDGGGSGRDEW------------------------LSSHELMLLANV 229
                      G S+   +   E                          S  E + LA  
Sbjct: 250 AKRRRKPIIRIGKSEFVDADETELPDPEPQPPPVPLLTELPVSEITPPSSEEETVSLAEE 309

Query: 230 TLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYESWRGSHFWEKAN 289
           TL+AWE +R G +KL+ +Y  +VC YC EVHVGP+GHKA+ CG FK++   G H W+ A 
Sbjct: 310 TLQAWEEMRAGAKKLMRMYRVRVCGYCPEVHVGPTGHKAQNCGAFKHQQRNGQHGWQSAV 369

Query: 290 VDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQAGVVAPSKYHCMMKIQ 317
           +DDL+PP  VWH    + P +  E R +YG  PAVV +C QAG V P  Y   M+++
Sbjct: 370 LDDLIPPRYVWHVPDVNGPPMQRELRSFYGQAPAVVEICAQAGAVVPEHYRATMRLE 426

BLAST of MS004875 vs. TAIR 10
Match: AT5G57930.2 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 217.2 bits (552), Expect = 1.9e-56
Identity = 111/297 (37.37%), Postives = 157/297 (52.86%), Query Frame = 0

Query: 50  IKAMIPVAQQVLEARAVLIHGVSTLLKAFPIVSCKFCPEVYVGEQGHLIRSCGGYKRGAK 109
           +K+++P+A +V  AR  LI+ +  L+K   + +C +C E++VG  GH  +SC G     +
Sbjct: 133 VKSLVPLAYKVYNARIRLINNLHRLMKVVRVNACGWCNEIHVGPYGHPFKSCKGPNTSQR 192

Query: 110 NRVHEWIGGDLNDVLVPVEAFHLHRMFQDVIKHDERFNFERVPAVAELCWQAGAN----- 169
             +HEW    + DV+VP+EA+HL       I+HDERF+  RVPAV ELC Q G       
Sbjct: 193 KGLHEWTNSVIEDVIVPLEAYHLFDRLGKRIRHDERFSIPRVPAVVELCIQGGVEIPEFP 252

Query: 170 -PNDENLSSSTGNSDGGGSGRDEW------------------------LSSHELMLLANV 229
                      G S+   +   E                          S  E + LA  
Sbjct: 253 AKRRRKPIIRIGKSEFVDADETELPDPEPQPPPVPLLTELPVSEITPPSSEEETVSLAEE 312

Query: 230 TLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSGHKARLCGVFKYESWRGSHFWEKAN 289
           TL+AWE +R G +KL+ +Y  +VC YC EVHVGP+GHKA+ CG FK++   G H W+ A 
Sbjct: 313 TLQAWEEMRAGAKKLMRMYRVRVCGYCPEVHVGPTGHKAQNCGAFKHQQRNGQHGWQSAV 372

Query: 290 VDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVVALCTQAGVVAPSKYHCMMKIQ 317
           +DDL+PP  VWH    + P +  E R +YG  PAVV +C QAG V P  Y   M+++
Sbjct: 373 LDDLIPPRYVWHVPDVNGPPMQRELRSFYGQAPAVVEICAQAGAVVPEHYRATMRLE 429

BLAST of MS004875 vs. TAIR 10
Match: AT1G64810.1 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 206.1 bits (523), Expect = 4.3e-53
Identity = 114/325 (35.08%), Postives = 164/325 (50.46%), Query Frame = 0

Query: 31  KKLRPM-ISKRIENRAKFYPIKAMIPVAQQVLEARAVLIHGVSTLLKAFPIVSCKFCPEV 90
           KKL  M I K+++       +  ++PVA QV++   +LI G++ LL   P+ +C  C  V
Sbjct: 103 KKLAQMGIEKQLDPPKNGLLVPNLVPVADQVIDNWKLLIKGLAQLLHVVPVFACSECGAV 162

Query: 91  YVGEQGHLIRSCGGYKRGAKNRVHEWIGGDLNDVLVPVEAFHLHRMFQDVIKHDERFNFE 150
           +V   GH IR C G     +   H W+ G +NDVL+PVE++H++  F   IKH+ RF +E
Sbjct: 163 HVANVGHNIRDCNGPTNSQRRGSHSWVKGTINDVLIPVESYHMYDPFGRRIKHETRFEYE 222

Query: 151 RVPAVAELCWQAGAN---------------------------------PNDENLSSSTGN 210
           R+PA+ ELC QAG                                       +LSS    
Sbjct: 223 RIPALVELCIQAGVEIPEYPCRRRTQPIRMMGKRVIDRGGYHKEPEKPQTSSSLSSPLAE 282

Query: 211 SDG-GGSGRDEWLSSHELMLLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSG 270
            D  G   R    +  ++  +A  T+ A+E +R GV KL+  +  K C YCSEVHVGP G
Sbjct: 283 LDTLGVFERYPPPTPEDIPKIAQETMDAYEKVRLGVTKLMRKFTVKACGYCSEVHVGPWG 342

Query: 271 HKARLCGVFKYESWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVV 321
           H  +LCG FK++   G H W+ A VD++ PPN VWH R      L    R +YG  PA+V
Sbjct: 343 HSVKLCGEFKHQWRDGKHGWQDALVDEVFPPNYVWHVRDLKGNPLTGNLRRFYGKAPALV 402

BLAST of MS004875 vs. TAIR 10
Match: AT1G64810.2 (Arabidopsis thaliana protein of unknown function (DUF794) )

HSP 1 Score: 206.1 bits (523), Expect = 4.3e-53
Identity = 114/325 (35.08%), Postives = 164/325 (50.46%), Query Frame = 0

Query: 31  KKLRPM-ISKRIENRAKFYPIKAMIPVAQQVLEARAVLIHGVSTLLKAFPIVSCKFCPEV 90
           KKL  M I K+++       +  ++PVA QV++   +LI G++ LL   P+ +C  C  V
Sbjct: 127 KKLAQMGIEKQLDPPKNGLLVPNLVPVADQVIDNWKLLIKGLAQLLHVVPVFACSECGAV 186

Query: 91  YVGEQGHLIRSCGGYKRGAKNRVHEWIGGDLNDVLVPVEAFHLHRMFQDVIKHDERFNFE 150
           +V   GH IR C G     +   H W+ G +NDVL+PVE++H++  F   IKH+ RF +E
Sbjct: 187 HVANVGHNIRDCNGPTNSQRRGSHSWVKGTINDVLIPVESYHMYDPFGRRIKHETRFEYE 246

Query: 151 RVPAVAELCWQAGAN---------------------------------PNDENLSSSTGN 210
           R+PA+ ELC QAG                                       +LSS    
Sbjct: 247 RIPALVELCIQAGVEIPEYPCRRRTQPIRMMGKRVIDRGGYHKEPEKPQTSSSLSSPLAE 306

Query: 211 SDG-GGSGRDEWLSSHELMLLANVTLKAWETLRTGVQKLLMVYPAKVCKYCSEVHVGPSG 270
            D  G   R    +  ++  +A  T+ A+E +R GV KL+  +  K C YCSEVHVGP G
Sbjct: 307 LDTLGVFERYPPPTPEDIPKIAQETMDAYEKVRLGVTKLMRKFTVKACGYCSEVHVGPWG 366

Query: 271 HKARLCGVFKYESWRGSHFWEKANVDDLVPPNIVWHRRPQDPPMLVDEGRDYYGHVPAVV 321
           H  +LCG FK++   G H W+ A VD++ PPN VWH R      L    R +YG  PA+V
Sbjct: 367 HSVKLCGEFKHQWRDGKHGWQDALVDEVFPPNYVWHVRDLKGNPLTGNLRRFYGKAPALV 426

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140768.12.2e-18499.37APO protein 4, mitochondrial [Momordica charantia][more]
XP_038901944.14.1e-16283.28APO protein 4, mitochondrial [Benincasa hispida] >XP_038901945.1 APO protein 4, ... [more]
XP_023532078.11.7e-16084.42APO protein 4, mitochondrial [Cucurbita pepo subsp. pepo][more]
XP_022971049.12.2e-16084.95APO protein 4, mitochondrial-like [Cucurbita maxima][more]
XP_004151879.17.2e-15983.54APO protein 4, mitochondrial [Cucumis sativus] >XP_031737269.1 APO protein 4, mi... [more]
Match NameE-valueIdentityDescription
Q9LSZ01.5e-10359.93APO protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=APO4 PE=2 SV=2[more]
Q8W4A52.6e-5537.37APO protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APO2 PE=2 SV=1[more]
Q9XIR46.1e-5235.08APO protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APO1 PE=2 SV=1[more]
Q9FH503.6e-4433.00APO protein 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=APO3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CIR91.1e-18499.37APO protein 4, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111011348 PE=4... [more]
A0A6J1I7G71.1e-16084.95APO protein 4, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111469835 PE... [more]
A0A0A0LQG53.5e-15983.54Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G421010 PE=4 SV=1[more]
A0A6J1G8H93.5e-15983.70APO protein 4, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111451698 ... [more]
A0A5A7TJ185.0e-15882.61APO protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1639G00180... [more]
Match NameE-valueIdentityDescription
AT3G21740.11.1e-10459.93Arabidopsis thaliana protein of unknown function (DUF794) [more]
AT5G57930.11.9e-5637.37Arabidopsis thaliana protein of unknown function (DUF794) [more]
AT5G57930.21.9e-5637.37Arabidopsis thaliana protein of unknown function (DUF794) [more]
AT1G64810.14.3e-5335.08Arabidopsis thaliana protein of unknown function (DUF794) [more]
AT1G64810.24.3e-5335.08Arabidopsis thaliana protein of unknown function (DUF794) [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023342APO domainPFAMPF05634APO_RNA-bindcoord: 26..164
e-value: 3.5E-40
score: 137.5
coord: 191..306
e-value: 7.6E-22
score: 77.7
IPR023342APO domainPROSITEPS51499APOcoord: 222..307
score: 21.735817
IPR023342APO domainPROSITEPS51499APOcoord: 82..167
score: 21.005489
NoneNo IPR availablePANTHERPTHR10388:SF20APO PROTEIN 4, MITOCHONDRIALcoord: 10..321
NoneNo IPR availablePANTHERPTHR10388EUKARYOTIC TRANSLATION INITIATION FACTOR SUI1coord: 10..321

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS004875.1MS004875.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003723 RNA binding