MS004866 (gene) Bitter gourd (TR) v1

Overview
NameMS004866
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionblue copper protein-like
Locationscaffold176: 1090280 .. 1091084 (-)
RNA-Seq ExpressionMS004866
SyntenyMS004866
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AGCATGGCCGCCTCAAGAACGCTATTGGCGTTGGCGGTTGCGGCCACAATGGTGGTTCAATTAGCCATGGCTGCTAATTACACAGTTGGAGATTCTGGTGGCTGGGAAATTGGCACCGATTTCAAATCTTGGGCTTCCTCCAAAAAATTCGTCGTTGGGGATGTTCTAAGTTGGTCCTTTCATTTTATTTCTTGCCCATTTTCAATTGTTTGTTTCTAATTGGAACCAAACTGAAATTTTACCGGTTGAATCCTTGACAGTTTTTGAGTACTCAGCGAACCACGATGTGCTTGAAGTTAATGAGGCAGATTTCAGCTCGTGTTCAGCGAGCAATCCGATTGAGAAGCACACCGACGGCAGCACAGCCATCGGTCTTTCCACCGCCGGCAAGAGATTCTTCATTTGTGGGACGCCCGGCCACTGCAGCACTGGAATGAAGATTGAAATCGACACCCTTGGAACTTCTTCGCCGCCGCCGGAAGCAACTCCGTCCCCTCCGCCGGCACCACCTACTTCTTCTCCGGCTGCACCCCCTAAAGCTCCTGCGAAACCCCCAACAAAATCTCCTCTGGCTCCACCACCTGATTCTCACGTGAGCCCGCCGGCGCCGCCTCCTGGACCAACTCCACGATCACCAGCGCCAGCACCGGAGGCGGAGTCTCCGACCCCTCCATTTCCATTTGACATGCCATTGAATCCTACAGCACCTCGCACAACTCCGCCGCCTTCATCGGCTTACAAATGCCGCTTCACGGCTCATCTCTCTGTTGGATTCAGCTTCGTCATCATAATGCTCTTGGCCATC

mRNA sequence

AGCATGGCCGCCTCAAGAACGCTATTGGCGTTGGCGGTTGCGGCCACAATGGTGGTTCAATTAGCCATGGCTGCTAATTACACAGTTGGAGATTCTGGTGGCTGGGAAATTGGCACCGATTTCAAATCTTGGGCTTCCTCCAAAAAATTCGTCGTTGGGGATGTTCTAATTTTTGAGTACTCAGCGAACCACGATGTGCTTGAAGTTAATGAGGCAGATTTCAGCTCGTGTTCAGCGAGCAATCCGATTGAGAAGCACACCGACGGCAGCACAGCCATCGGTCTTTCCACCGCCGGCAAGAGATTCTTCATTTGTGGGACGCCCGGCCACTGCAGCACTGGAATGAAGATTGAAATCGACACCCTTGGAACTTCTTCGCCGCCGCCGGAAGCAACTCCGTCCCCTCCGCCGGCACCACCTACTTCTTCTCCGGCTGCACCCCCTAAAGCTCCTGCGAAACCCCCAACAAAATCTCCTCTGGCTCCACCACCTGATTCTCACGTGAGCCCGCCGGCGCCGCCTCCTGGACCAACTCCACGATCACCAGCGCCAGCACCGGAGGCGGAGTCTCCGACCCCTCCATTTCCATTTGACATGCCATTGAATCCTACAGCACCTCGCACAACTCCGCCGCCTTCATCGGCTTACAAATGCCGCTTCACGGCTCATCTCTCTGTTGGATTCAGCTTCGTCATCATAATGCTCTTGGCCATC

Coding sequence (CDS)

AGCATGGCCGCCTCAAGAACGCTATTGGCGTTGGCGGTTGCGGCCACAATGGTGGTTCAATTAGCCATGGCTGCTAATTACACAGTTGGAGATTCTGGTGGCTGGGAAATTGGCACCGATTTCAAATCTTGGGCTTCCTCCAAAAAATTCGTCGTTGGGGATGTTCTAATTTTTGAGTACTCAGCGAACCACGATGTGCTTGAAGTTAATGAGGCAGATTTCAGCTCGTGTTCAGCGAGCAATCCGATTGAGAAGCACACCGACGGCAGCACAGCCATCGGTCTTTCCACCGCCGGCAAGAGATTCTTCATTTGTGGGACGCCCGGCCACTGCAGCACTGGAATGAAGATTGAAATCGACACCCTTGGAACTTCTTCGCCGCCGCCGGAAGCAACTCCGTCCCCTCCGCCGGCACCACCTACTTCTTCTCCGGCTGCACCCCCTAAAGCTCCTGCGAAACCCCCAACAAAATCTCCTCTGGCTCCACCACCTGATTCTCACGTGAGCCCGCCGGCGCCGCCTCCTGGACCAACTCCACGATCACCAGCGCCAGCACCGGAGGCGGAGTCTCCGACCCCTCCATTTCCATTTGACATGCCATTGAATCCTACAGCACCTCGCACAACTCCGCCGCCTTCATCGGCTTACAAATGCCGCTTCACGGCTCATCTCTCTGTTGGATTCAGCTTCGTCATCATAATGCTCTTGGCCATC

Protein sequence

SMAASRTLLALAVAATMVVQLAMAANYTVGDSGGWEIGTDFKSWASSKKFVVGDVLIFEYSANHDVLEVNEADFSSCSASNPIEKHTDGSTAIGLSTAGKRFFICGTPGHCSTGMKIEIDTLGTSSPPPEATPSPPPAPPTSSPAAPPKAPAKPPTKSPLAPPPDSHVSPPAPPPGPTPRSPAPAPEAESPTPPFPFDMPLNPTAPRTTPPPSSAYKCRFTAHLSVGFSFVIIMLLAI
Homology
BLAST of MS004866 vs. NCBI nr
Match: XP_022140290.1 (blue copper protein-like [Momordica charantia])

HSP 1 Score: 420.6 bits (1080), Expect = 8.9e-114
Identity = 234/240 (97.50%), Postives = 234/240 (97.50%), Query Frame = 0

Query: 2   MAASRTLLALAVAATMVVQLAMAANYTVGDSGGWEIGTDFKSWASSKKFVVGDVLIFEYS 61
           MAASRTLLALAVAATMVVQLAMAANYTVGDSGGWEIGTDFKSWASSKKFVVGDVLIFEYS
Sbjct: 1   MAASRTLLALAVAATMVVQLAMAANYTVGDSGGWEIGTDFKSWASSKKFVVGDVLIFEYS 60

Query: 62  ANHDVLEVNEADFSSCSASNPIEKHTDGSTAIGLSTAGKRFFICGTPGHCSTGMKIEIDT 121
           ANHDVLEVNEADFSSCSASNPIEKHTDGSTAIGLSTAGKRFFICGTPGHCSTGMKIEIDT
Sbjct: 61  ANHDVLEVNEADFSSCSASNPIEKHTDGSTAIGLSTAGKRFFICGTPGHCSTGMKIEIDT 120

Query: 122 LGTSSPPPEATPSPPPAPPTSSPAAPPKAPAKPPTKSPLAPPPDSHVSPPAPPPGPTPRS 181
           LGTSSPPPEATPSPPPAPPTSSPAAPPKA AKPPTKSPLAPPPDSHVSPPAPPPGPTPRS
Sbjct: 121 LGTSSPPPEATPSPPPAPPTSSPAAPPKAAAKPPTKSPLAPPPDSHVSPPAPPPGPTPRS 180

Query: 182 PAPAPEAESPTPPFPFDMPLNPTAPRTT---PPPSSAYKCRFTAHLSVGFSFVIIMLLAI 239
           PAPAPEAESPTPP PFDMPLNPTAP TT   PPPSSAYKCRFTAHLSVGFSFVIIMLLAI
Sbjct: 181 PAPAPEAESPTPPXPFDMPLNPTAPPTTPPPPPPSSAYKCRFTAHLSVGFSFVIIMLLAI 240

BLAST of MS004866 vs. NCBI nr
Match: XP_038900873.1 (blue copper protein-like [Benincasa hispida])

HSP 1 Score: 297.7 bits (761), Expect = 8.7e-77
Identity = 182/256 (71.09%), Postives = 197/256 (76.95%), Query Frame = 0

Query: 2   MAASRTL-LALAVAATMVVQLAMAANYTVGDSGGWEIGTDFKSWASSKKFVVGDVLIFEY 61
           MAASRTL +ALA+AATMVV+LAMA NYTVGDSGGWEIG +F+SWASSK F +GDVLIFEY
Sbjct: 5   MAASRTLIMALAIAATMVVELAMATNYTVGDSGGWEIGANFQSWASSKNFTIGDVLIFEY 64

Query: 62  SANHDVLEVNEADFSSCSASNPIEKHTDGSTAIGLSTAGKRFFICGTPGHCSTGMKIEID 121
           SANHDVLEVNE DFSSCSASNPIEKHT GST I L T+GKRFFICG PGHC +GMK+EID
Sbjct: 65  SANHDVLEVNEPDFSSCSASNPIEKHTGGSTVITLLTSGKRFFICGVPGHCLSGMKVEID 124

Query: 122 TLGT-----------SSPPPEATPSPP-----PAPPTSSPAAPPKAPAKPPTKSPLAPPP 181
           TL T            SPPPE TPS P     PA PTS PA+PPKA  KPP KSPLAPPP
Sbjct: 125 TLATPSPPPSSMATPPSPPPEVTPSSPPPTSSPAAPTSPPASPPKAATKPPAKSPLAPPP 184

Query: 182 DSHVSPPAPPPGPTPRSPAPAPEAESPTPPFPFDMPLNPTAPRTTPP--PSSAYKCRFTA 239
           DS  SPPAPP  PTP  P+ AP  E P PPFPFD PL P+A    PP  PSSAYKC F  
Sbjct: 185 DSKASPPAPPTEPTP--PSSAPTLEPPIPPFPFDHPLIPSAAPIPPPPTPSSAYKCSFRV 244

BLAST of MS004866 vs. NCBI nr
Match: XP_022948200.1 (blue copper protein-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 287.0 bits (733), Expect = 1.5e-73
Identity = 174/257 (67.70%), Postives = 194/257 (75.49%), Query Frame = 0

Query: 2   MAASRTLLALAVAATMVVQLAMAANYTVGDSGGWEIGTDFKSWASSKKFVVGDVLIFEYS 61
           MAASRTL+ALA+AAT+VV+LAMAANYTVGDS GWEIGTD +SWA+SK F VGDV+ F YS
Sbjct: 1   MAASRTLMALAIAATLVVELAMAANYTVGDSDGWEIGTDVQSWAASKNFTVGDVISFVYS 60

Query: 62  ANHDVLEVNEADFSSCSASNPIEKHTDGSTAIGLSTAGKRFFICGTPGHCSTGMKIEIDT 121
           ANHDV+EV E DF SCS SNPIEKHT G+TAI LSTAGKRFFICG PGHC+ GMK++IDT
Sbjct: 61  ANHDVVEVKEVDFGSCSGSNPIEKHTGGNTAITLSTAGKRFFICGVPGHCTAGMKVQIDT 120

Query: 122 LGTSSPPPEAT---PSPPP---------------APPTSSPAAPPKAPAKPPTKSPLAPP 181
           L  SSPPP +T   PSPPP               AP +  PA+PPK+  KPPTKSPLAP 
Sbjct: 121 LAASSPPPSSTDAPPSPPPHATPSSPPPASPPLVAPSSPPPASPPKSATKPPTKSPLAPS 180

Query: 182 PDSHVSPPAPPPGPTPRSPAPAPEAESPTPPFPFDMPLNPTAPRTT--PPPSSAYKCRFT 239
           P S+ SPPAPP  PTP S AP P  E P PPFPFD PL P+A  TT  PPPSSAYKC F 
Sbjct: 181 PGSNASPPAPPFKPTPPSSAPTP--EPPIPPFPFDQPLIPSAAPTTPPPPPSSAYKCSFM 240

BLAST of MS004866 vs. NCBI nr
Match: XP_022948201.1 (blue copper protein-like isoform X4 [Cucurbita moschata])

HSP 1 Score: 286.2 bits (731), Expect = 2.6e-73
Identity = 171/245 (69.80%), Postives = 190/245 (77.55%), Query Frame = 0

Query: 2   MAASRTLLALAVAATMVVQLAMAANYTVGDSGGWEIGTDFKSWASSKKFVVGDVLIFEYS 61
           MAASRTL+ALA+AAT+VV+LAMAANYTVGDS GWEIGTD +SWA+SK F VGDV+ F YS
Sbjct: 1   MAASRTLMALAIAATLVVELAMAANYTVGDSDGWEIGTDVQSWAASKNFTVGDVISFVYS 60

Query: 62  ANHDVLEVNEADFSSCSASNPIEKHTDGSTAIGLSTAGKRFFICGTPGHCSTGMKIEIDT 121
           ANHDV+EV E DF SCS SNPIEKHT G+TAI LSTAGKRFFICG PGHC+ GMK++IDT
Sbjct: 61  ANHDVVEVKEVDFGSCSGSNPIEKHTGGNTAITLSTAGKRFFICGVPGHCTAGMKVQIDT 120

Query: 122 LGTSSPPPEATPSPPP------APPTSSPAAPPKAPAKPPTKSPLAPPPDSHVSPPAPPP 181
           L  SSPP     SPPP      AP +  PA+PPK+  KPPTKSPLAP P S+ SPPAPP 
Sbjct: 121 LAASSPPHATPSSPPPASPPLVAPSSPPPASPPKSATKPPTKSPLAPSPGSNASPPAPPF 180

Query: 182 GPTPRSPAPAPEAESPTPPFPFDMPLNPTAPRTT--PPPSSAYKCRFTAHLSVGFSFVII 239
            PTP S AP P  E P PPFPFD PL P+A  TT  PPPSSAYKC F  H SVGFSFV I
Sbjct: 181 KPTPPSSAPTP--EPPIPPFPFDQPLIPSAAPTTPPPPPSSAYKCSFMVHFSVGFSFVAI 240

BLAST of MS004866 vs. NCBI nr
Match: XP_022948199.1 (blue copper protein-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 283.5 bits (724), Expect = 1.7e-72
Identity = 174/257 (67.70%), Postives = 192/257 (74.71%), Query Frame = 0

Query: 2   MAASRTLLALAVAATMVVQLAMAANYTVGDSGGWEIGTDFKSWASSKKFVVGDVLIFEYS 61
           MAASRTL+ALA+AAT+VV+LAMAANYTVGDS GWEIGTD +SWA+SK F VGDV+ F YS
Sbjct: 1   MAASRTLMALAIAATLVVELAMAANYTVGDSDGWEIGTDVQSWAASKNFTVGDVISFVYS 60

Query: 62  ANHDVLEVNEADFSSCSASNPIEKHTDGSTAIGLSTAGKRFFICGTPGHCSTGMKIEIDT 121
           ANHDV+EV E DF SCS SNPIEKHT G+TAI LSTAGKRFFICG PGHC+ GMK++IDT
Sbjct: 61  ANHDVVEVKEVDFGSCSGSNPIEKHTGGNTAITLSTAGKRFFICGVPGHCTAGMKVQIDT 120

Query: 122 LGTSSPPPEATPSPPPA-PPTSS-----------------PAAPPKAPAKPPTKSPLAPP 181
           L  SSPP     SPPPA PP SS                 PA+PPK+  KPPTKSPLAP 
Sbjct: 121 LAASSPPHATPSSPPPASPPPSSKDAPPTPPPIVAPSSPPPASPPKSATKPPTKSPLAPS 180

Query: 182 PDSHVSPPAPPPGPTPRSPAPAPEAESPTPPFPFDMPLNPTAPRTT--PPPSSAYKCRFT 239
           P S+ SPPAPP  PTP S AP P  E P PPFPFD PL P+A  TT  PPPSSAYKC F 
Sbjct: 181 PGSNASPPAPPFKPTPPSSAPTP--EPPIPPFPFDQPLIPSAAPTTPPPPPSSAYKCSFM 240

BLAST of MS004866 vs. ExPASy Swiss-Prot
Match: O80517 (Uclacyanin-2 OS=Arabidopsis thaliana OX=3702 GN=At2g44790 PE=2 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 6.6e-19
Identity = 71/189 (37.57%), Postives = 101/189 (53.44%), Query Frame = 0

Query: 5   SRTLLALAVAATMVVQL---AMAANYTVGDSGGWEIGTDFKSWASSKKFVVGDVLIFEYS 64
           S+  +A A A  +V+ +   A+A  YT+     W  G D+  WA+ K F VGD+L F+Y 
Sbjct: 7   SKMAVAAATALLLVLTIVPGAVAVTYTI----EWTTGVDYSGWATGKTFRVGDILEFKYG 66

Query: 65  ANHDVLEVNEADFSSCSASNPIEKHTDGSTAIGLSTAGKRFFICGTPGHCST--GMKIEI 124
           ++H V  V++A +  C AS+  E H+DG T I L T G  +FIC TPGHC T  GMK+ +
Sbjct: 67  SSHTVDVVDKAGYDGCDASSSTENHSDGDTKIDLKTVGINYFICSTPGHCRTNGGMKLAV 126

Query: 125 DTLGTSSPPPEATPSPPPAPPTSSPAAPPKAPAKPPTKSPLAPPPDSHVSPPAPPPGPTP 184
           + +  S+ PP       P PP+S+P  P          +P +PP     +P  P PG   
Sbjct: 127 NVVAGSAGPPAT-----PTPPSSTPGTP---------TTPESPPSGGSPTPTTPTPGAGS 177

Query: 185 RSPAPAPEA 189
            SP P P+A
Sbjct: 187 TSPPPPPKA 177

BLAST of MS004866 vs. ExPASy Swiss-Prot
Match: O82081 (Uclacyanin 1 OS=Arabidopsis thaliana OX=3702 GN=UCC1 PE=1 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 1.5e-18
Identity = 78/217 (35.94%), Postives = 123/217 (56.68%), Query Frame = 0

Query: 2   MAASRTLLALAVAATMVVQLAMAANYTVGDSGGWEIGTDFKSWASSKKFVVGDVLIFEY- 61
           MA+   L+ ++V AT ++ L +A ++T+G   GW +G   ++WA+ + F VGD L+F Y 
Sbjct: 1   MASREMLIIISVLATTLIGLTVATDHTIGGPSGWTVGASLRTWAAGQTFAVGDNLVFSYP 60

Query: 62  SANHDVLEVNEADFSSCSASNPIEKHTDGSTAIGLSTAGKRFFICGTPGHCSTGMKIEID 121
           +A HDV+EV + +F SC A  P+    +G++ + L+T GKR+FICG PGHCS GMK+E++
Sbjct: 61  AAFHDVVEVTKPEFDSCQAVKPLITFANGNSLVPLTTPGKRYFICGMPGHCSQGMKLEVN 120

Query: 122 TLGTSSPPPEATPSPPPAPPTSSPAAP---PKAPAKPPTKSPLAPPPDSHVSPPAPPPGP 181
            + T++  P A P P   P  ++P+     P  P  P    P+  P  S   P +  P  
Sbjct: 121 VVPTATVAPTA-PLPNTVPSLNAPSPSSVLPIQPLLPLNPVPVLSPSSSTPLPSSSLPLI 180

Query: 182 TPRSPAPAPEAESPTPPFPFDMPLNPTAPRTTPPPSS 215
            P SPA +P   + T      +PL P +P ++   +S
Sbjct: 181 PPLSPALSPATAAGT-----SLPLFPGSPGSSSSTTS 211

BLAST of MS004866 vs. ExPASy Swiss-Prot
Match: Q41001 (Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 4.3e-18
Identity = 69/161 (42.86%), Postives = 90/161 (55.90%), Query Frame = 0

Query: 2   MAASRTLLALAVAATMVVQL-AMAANYTVGDSGGWEIGTDFKSWASSKKFVVGDVLIFEY 61
           MA S  L+   + A + + L ++A  YTVGD+ GW IG D+ +WAS K F VGD L+F Y
Sbjct: 1   MAFSNALVLCFLLAIINMALPSLATVYTVGDTSGWVIGGDYSTWASDKTFAVGDSLVFNY 60

Query: 62  SAN-HDVLEVNEADFSSCSASNPIEKHTDGSTAIGLSTAGKRFFICGTPGHCSTGMKIEI 121
            A  H V EV E+D+ SC++ N I   + G+T I L  AGK +FICG PGH + GMK+ I
Sbjct: 61  GAGAHTVDEVKESDYKSCTSGNSISTDSTGATTIPLKKAGKHYFICGVPGHSTGGMKLSI 120

Query: 122 DTLGT--SSPPPEATPSPP-PAPPTSSPAAPPKAPAKPPTK 158
               +  SS  P ATPS      P+S            PTK
Sbjct: 121 KVKASSGSSAAPSATPSSSGKGSPSSDDTPAATTTTTTPTK 161

BLAST of MS004866 vs. ExPASy Swiss-Prot
Match: P80728 (Mavicyanin OS=Cucurbita pepo OX=3663 PE=1 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 4.3e-18
Identity = 45/99 (45.45%), Postives = 63/99 (63.64%), Query Frame = 0

Query: 24  AANYTVGDSGGWE--IGTDFKSWASSKKFVVGDVLIFEYSAN-HDVLEVNEADFSSCSAS 83
           A  + VGDS GW   +  D+  WASS KF VGD L+F Y+   H+VL+V++  F SC++S
Sbjct: 1   ATVHKVGDSTGWTTLVPYDYAKWASSNKFHVGDSLLFNYNNKFHNVLQVDQEQFKSCNSS 60

Query: 84  NPIEKHTDGSTAIGLSTAGKRFFICGTPGHCSTGMKIEI 120
           +P   +T G+ +I L   G  +F+CG PGHC  G K+EI
Sbjct: 61  SPAASYTSGADSIPLKRPGTFYFLCGIPGHCQLGQKVEI 99

BLAST of MS004866 vs. ExPASy Swiss-Prot
Match: P00302 (Stellacyanin OS=Toxicodendron vernicifluum OX=4013 PE=1 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 8.0e-17
Identity = 42/101 (41.58%), Postives = 63/101 (62.38%), Query Frame = 0

Query: 27  YTVGDSGGW------EIGTDFKSWASSKKFVVGDVLIFEYSAN-HDVLEVNEADFSSCSA 86
           YTVGDS GW      ++  D+K WAS+K F +GDVL+F+Y    H+V +V + ++ SC+ 
Sbjct: 3   YTVGDSAGWKVPFFGDVDYDWK-WASNKTFHIGDVLVFKYDRRFHNVDKVTQKNYQSCND 62

Query: 87  SNPIEKHTDGSTAIGLSTAGKRFFICGTPGHCSTGMKIEID 121
           + PI  +  G   I L T G++++ICG P HC  G K+ I+
Sbjct: 63  TTPIASYNTGBBRINLKTVGQKYYICGVPKHCDLGQKVHIN 102

BLAST of MS004866 vs. ExPASy TrEMBL
Match: A0A6J1CEP4 (blue copper protein-like OS=Momordica charantia OX=3673 GN=LOC111010993 PE=4 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 4.3e-114
Identity = 234/240 (97.50%), Postives = 234/240 (97.50%), Query Frame = 0

Query: 2   MAASRTLLALAVAATMVVQLAMAANYTVGDSGGWEIGTDFKSWASSKKFVVGDVLIFEYS 61
           MAASRTLLALAVAATMVVQLAMAANYTVGDSGGWEIGTDFKSWASSKKFVVGDVLIFEYS
Sbjct: 1   MAASRTLLALAVAATMVVQLAMAANYTVGDSGGWEIGTDFKSWASSKKFVVGDVLIFEYS 60

Query: 62  ANHDVLEVNEADFSSCSASNPIEKHTDGSTAIGLSTAGKRFFICGTPGHCSTGMKIEIDT 121
           ANHDVLEVNEADFSSCSASNPIEKHTDGSTAIGLSTAGKRFFICGTPGHCSTGMKIEIDT
Sbjct: 61  ANHDVLEVNEADFSSCSASNPIEKHTDGSTAIGLSTAGKRFFICGTPGHCSTGMKIEIDT 120

Query: 122 LGTSSPPPEATPSPPPAPPTSSPAAPPKAPAKPPTKSPLAPPPDSHVSPPAPPPGPTPRS 181
           LGTSSPPPEATPSPPPAPPTSSPAAPPKA AKPPTKSPLAPPPDSHVSPPAPPPGPTPRS
Sbjct: 121 LGTSSPPPEATPSPPPAPPTSSPAAPPKAAAKPPTKSPLAPPPDSHVSPPAPPPGPTPRS 180

Query: 182 PAPAPEAESPTPPFPFDMPLNPTAPRTT---PPPSSAYKCRFTAHLSVGFSFVIIMLLAI 239
           PAPAPEAESPTPP PFDMPLNPTAP TT   PPPSSAYKCRFTAHLSVGFSFVIIMLLAI
Sbjct: 181 PAPAPEAESPTPPXPFDMPLNPTAPPTTPPPPPPSSAYKCRFTAHLSVGFSFVIIMLLAI 240

BLAST of MS004866 vs. ExPASy TrEMBL
Match: A0A6J1G946 (blue copper protein-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111451847 PE=4 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 7.4e-74
Identity = 174/257 (67.70%), Postives = 194/257 (75.49%), Query Frame = 0

Query: 2   MAASRTLLALAVAATMVVQLAMAANYTVGDSGGWEIGTDFKSWASSKKFVVGDVLIFEYS 61
           MAASRTL+ALA+AAT+VV+LAMAANYTVGDS GWEIGTD +SWA+SK F VGDV+ F YS
Sbjct: 1   MAASRTLMALAIAATLVVELAMAANYTVGDSDGWEIGTDVQSWAASKNFTVGDVISFVYS 60

Query: 62  ANHDVLEVNEADFSSCSASNPIEKHTDGSTAIGLSTAGKRFFICGTPGHCSTGMKIEIDT 121
           ANHDV+EV E DF SCS SNPIEKHT G+TAI LSTAGKRFFICG PGHC+ GMK++IDT
Sbjct: 61  ANHDVVEVKEVDFGSCSGSNPIEKHTGGNTAITLSTAGKRFFICGVPGHCTAGMKVQIDT 120

Query: 122 LGTSSPPPEAT---PSPPP---------------APPTSSPAAPPKAPAKPPTKSPLAPP 181
           L  SSPPP +T   PSPPP               AP +  PA+PPK+  KPPTKSPLAP 
Sbjct: 121 LAASSPPPSSTDAPPSPPPHATPSSPPPASPPLVAPSSPPPASPPKSATKPPTKSPLAPS 180

Query: 182 PDSHVSPPAPPPGPTPRSPAPAPEAESPTPPFPFDMPLNPTAPRTT--PPPSSAYKCRFT 239
           P S+ SPPAPP  PTP S AP P  E P PPFPFD PL P+A  TT  PPPSSAYKC F 
Sbjct: 181 PGSNASPPAPPFKPTPPSSAPTP--EPPIPPFPFDQPLIPSAAPTTPPPPPSSAYKCSFM 240

BLAST of MS004866 vs. ExPASy TrEMBL
Match: A0A6J1G8L4 (blue copper protein-like isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111451847 PE=4 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 1.3e-73
Identity = 171/245 (69.80%), Postives = 190/245 (77.55%), Query Frame = 0

Query: 2   MAASRTLLALAVAATMVVQLAMAANYTVGDSGGWEIGTDFKSWASSKKFVVGDVLIFEYS 61
           MAASRTL+ALA+AAT+VV+LAMAANYTVGDS GWEIGTD +SWA+SK F VGDV+ F YS
Sbjct: 1   MAASRTLMALAIAATLVVELAMAANYTVGDSDGWEIGTDVQSWAASKNFTVGDVISFVYS 60

Query: 62  ANHDVLEVNEADFSSCSASNPIEKHTDGSTAIGLSTAGKRFFICGTPGHCSTGMKIEIDT 121
           ANHDV+EV E DF SCS SNPIEKHT G+TAI LSTAGKRFFICG PGHC+ GMK++IDT
Sbjct: 61  ANHDVVEVKEVDFGSCSGSNPIEKHTGGNTAITLSTAGKRFFICGVPGHCTAGMKVQIDT 120

Query: 122 LGTSSPPPEATPSPPP------APPTSSPAAPPKAPAKPPTKSPLAPPPDSHVSPPAPPP 181
           L  SSPP     SPPP      AP +  PA+PPK+  KPPTKSPLAP P S+ SPPAPP 
Sbjct: 121 LAASSPPHATPSSPPPASPPLVAPSSPPPASPPKSATKPPTKSPLAPSPGSNASPPAPPF 180

Query: 182 GPTPRSPAPAPEAESPTPPFPFDMPLNPTAPRTT--PPPSSAYKCRFTAHLSVGFSFVII 239
            PTP S AP P  E P PPFPFD PL P+A  TT  PPPSSAYKC F  H SVGFSFV I
Sbjct: 181 KPTPPSSAPTP--EPPIPPFPFDQPLIPSAAPTTPPPPPSSAYKCSFMVHFSVGFSFVAI 240

BLAST of MS004866 vs. ExPASy TrEMBL
Match: A0A6J1G963 (blue copper protein-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111451847 PE=4 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 8.2e-73
Identity = 174/257 (67.70%), Postives = 192/257 (74.71%), Query Frame = 0

Query: 2   MAASRTLLALAVAATMVVQLAMAANYTVGDSGGWEIGTDFKSWASSKKFVVGDVLIFEYS 61
           MAASRTL+ALA+AAT+VV+LAMAANYTVGDS GWEIGTD +SWA+SK F VGDV+ F YS
Sbjct: 1   MAASRTLMALAIAATLVVELAMAANYTVGDSDGWEIGTDVQSWAASKNFTVGDVISFVYS 60

Query: 62  ANHDVLEVNEADFSSCSASNPIEKHTDGSTAIGLSTAGKRFFICGTPGHCSTGMKIEIDT 121
           ANHDV+EV E DF SCS SNPIEKHT G+TAI LSTAGKRFFICG PGHC+ GMK++IDT
Sbjct: 61  ANHDVVEVKEVDFGSCSGSNPIEKHTGGNTAITLSTAGKRFFICGVPGHCTAGMKVQIDT 120

Query: 122 LGTSSPPPEATPSPPPA-PPTSS-----------------PAAPPKAPAKPPTKSPLAPP 181
           L  SSPP     SPPPA PP SS                 PA+PPK+  KPPTKSPLAP 
Sbjct: 121 LAASSPPHATPSSPPPASPPPSSKDAPPTPPPIVAPSSPPPASPPKSATKPPTKSPLAPS 180

Query: 182 PDSHVSPPAPPPGPTPRSPAPAPEAESPTPPFPFDMPLNPTAPRTT--PPPSSAYKCRFT 239
           P S+ SPPAPP  PTP S AP P  E P PPFPFD PL P+A  TT  PPPSSAYKC F 
Sbjct: 181 PGSNASPPAPPFKPTPPSSAPTP--EPPIPPFPFDQPLIPSAAPTTPPPPPSSAYKCSFM 240

BLAST of MS004866 vs. ExPASy TrEMBL
Match: A0A6J1G8Q5 (blue copper protein-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111451847 PE=4 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 1.1e-72
Identity = 176/269 (65.43%), Postives = 196/269 (72.86%), Query Frame = 0

Query: 2   MAASRTLLALAVAATMVVQLAMAANYTVGDSGGWEIGTDFKSWASSKKFVVGDVLIFEYS 61
           MAASRTL+ALA+AAT+VV+LAMAANYTVGDS GWEIGTD +SWA+SK F VGDV+ F YS
Sbjct: 1   MAASRTLMALAIAATLVVELAMAANYTVGDSDGWEIGTDVQSWAASKNFTVGDVISFVYS 60

Query: 62  ANHDVLEVNEADFSSCSASNPIEKHTDGSTAIGLSTAGKRFFICGTPGHCSTGMKIEIDT 121
           ANHDV+EV E DF SCS SNPIEKHT G+TAI LSTAGKRFFICG PGHC+ GMK++IDT
Sbjct: 61  ANHDVVEVKEVDFGSCSGSNPIEKHTGGNTAITLSTAGKRFFICGVPGHCTAGMKVQIDT 120

Query: 122 LGTSSPPPEAT---PSPPP----------APPTSS-----------------PAAPPKAP 181
           L  SSPPP +T   PSPPP          +PP SS                 PA+PPK+ 
Sbjct: 121 LAASSPPPSSTDAPPSPPPHATPSSPPPASPPPSSKDAPPTPPPIVAPSSPPPASPPKSA 180

Query: 182 AKPPTKSPLAPPPDSHVSPPAPPPGPTPRSPAPAPEAESPTPPFPFDMPLNPTAPRTT-- 239
            KPPTKSPLAP P S+ SPPAPP  PTP S AP P  E P PPFPFD PL P+A  TT  
Sbjct: 181 TKPPTKSPLAPSPGSNASPPAPPFKPTPPSSAPTP--EPPIPPFPFDQPLIPSAAPTTPP 240

BLAST of MS004866 vs. TAIR 10
Match: AT3G60270.1 (Cupredoxin superfamily protein )

HSP 1 Score: 109.8 bits (273), Expect = 3.1e-24
Identity = 67/155 (43.23%), Postives = 89/155 (57.42%), Query Frame = 0

Query: 1   SMAASRTLLALAVAATMVVQLAMAANYTVGDSGGWEIGTDFKSWASSKKFVVGDVLIFEY 60
           S AA+  LL L + A   V    A  + VGD+ GW IG ++ SW S K F VGD L F+Y
Sbjct: 3   STAATALLLLLLLVA---VPAVFAVTFQVGDNDGWTIGVEYTSWVSEKTFRVGDTLEFKY 62

Query: 61  SANHDVLEVNEADFSSCSASNPIEKHTDGSTAIGLSTAGKRFFICGTPGHCSTGMKIEID 120
             +H V  VN+AD+  C  S P +  +DG T I L+  G   F+C TPGHCS GMK+ + 
Sbjct: 63  GPSHSVAVVNKADYDGCETSRPTQSFSDGDTKIDLTKVGAIHFLCLTPGHCSLGMKLAVQ 122

Query: 121 TLGTSS---PPPEATPSPPPAPPTSSPAAPPKAPA 153
            L   S   PP  + PSP P+ P+ SP+AP  +P+
Sbjct: 123 VLAAVSLEPPPSPSAPSPSPSAPSPSPSAPSPSPS 154

BLAST of MS004866 vs. TAIR 10
Match: AT2G44790.1 (uclacyanin 2 )

HSP 1 Score: 95.9 bits (237), Expect = 4.7e-20
Identity = 71/189 (37.57%), Postives = 101/189 (53.44%), Query Frame = 0

Query: 5   SRTLLALAVAATMVVQL---AMAANYTVGDSGGWEIGTDFKSWASSKKFVVGDVLIFEYS 64
           S+  +A A A  +V+ +   A+A  YT+     W  G D+  WA+ K F VGD+L F+Y 
Sbjct: 7   SKMAVAAATALLLVLTIVPGAVAVTYTI----EWTTGVDYSGWATGKTFRVGDILEFKYG 66

Query: 65  ANHDVLEVNEADFSSCSASNPIEKHTDGSTAIGLSTAGKRFFICGTPGHCST--GMKIEI 124
           ++H V  V++A +  C AS+  E H+DG T I L T G  +FIC TPGHC T  GMK+ +
Sbjct: 67  SSHTVDVVDKAGYDGCDASSSTENHSDGDTKIDLKTVGINYFICSTPGHCRTNGGMKLAV 126

Query: 125 DTLGTSSPPPEATPSPPPAPPTSSPAAPPKAPAKPPTKSPLAPPPDSHVSPPAPPPGPTP 184
           + +  S+ PP       P PP+S+P  P          +P +PP     +P  P PG   
Sbjct: 127 NVVAGSAGPPAT-----PTPPSSTPGTP---------TTPESPPSGGSPTPTTPTPGAGS 177

Query: 185 RSPAPAPEA 189
            SP P P+A
Sbjct: 187 TSPPPPPKA 177

BLAST of MS004866 vs. TAIR 10
Match: AT2G32300.1 (uclacyanin 1 )

HSP 1 Score: 94.7 bits (234), Expect = 1.0e-19
Identity = 78/217 (35.94%), Postives = 123/217 (56.68%), Query Frame = 0

Query: 2   MAASRTLLALAVAATMVVQLAMAANYTVGDSGGWEIGTDFKSWASSKKFVVGDVLIFEY- 61
           MA+   L+ ++V AT ++ L +A ++T+G   GW +G   ++WA+ + F VGD L+F Y 
Sbjct: 1   MASREMLIIISVLATTLIGLTVATDHTIGGPSGWTVGASLRTWAAGQTFAVGDNLVFSYP 60

Query: 62  SANHDVLEVNEADFSSCSASNPIEKHTDGSTAIGLSTAGKRFFICGTPGHCSTGMKIEID 121
           +A HDV+EV + +F SC A  P+    +G++ + L+T GKR+FICG PGHCS GMK+E++
Sbjct: 61  AAFHDVVEVTKPEFDSCQAVKPLITFANGNSLVPLTTPGKRYFICGMPGHCSQGMKLEVN 120

Query: 122 TLGTSSPPPEATPSPPPAPPTSSPAAP---PKAPAKPPTKSPLAPPPDSHVSPPAPPPGP 181
            + T++  P A P P   P  ++P+     P  P  P    P+  P  S   P +  P  
Sbjct: 121 VVPTATVAPTA-PLPNTVPSLNAPSPSSVLPIQPLLPLNPVPVLSPSSSTPLPSSSLPLI 180

Query: 182 TPRSPAPAPEAESPTPPFPFDMPLNPTAPRTTPPPSS 215
            P SPA +P   + T      +PL P +P ++   +S
Sbjct: 181 PPLSPALSPATAAGT-----SLPLFPGSPGSSSSTTS 211

BLAST of MS004866 vs. TAIR 10
Match: AT5G07475.1 (Cupredoxin superfamily protein )

HSP 1 Score: 90.1 bits (222), Expect = 2.6e-18
Identity = 53/144 (36.81%), Postives = 79/144 (54.86%), Query Frame = 0

Query: 13  VAATMVVQLAMAANYTVGDSGGWEIGTDFKSWASSKKFVVGDVLIFEYSANHDVLEVNEA 72
           +   +V++   A  Y VGDS GW+I +D +SW S K+F  GDVL+F+YS+ H V EV + 
Sbjct: 16  IFGVVVIRRCNATTYFVGDSSGWDISSDLESWTSGKRFSPGDVLMFQYSSTHSVYEVAKD 75

Query: 73  DFSSCSASNPIEKHTDGSTAIGLSTAGKRFFICGTPGHCSTGMKIEIDTLGTSSPPPEAT 132
           ++ +C+ ++ I   T+G+T + LS  G RFF+CG   HC  GM++ ++  G     P   
Sbjct: 76  NYQNCNTTDAIRTFTNGNTTVALSKPGNRFFVCGNRLHCFAGMRLLVNVEGNG---PSQA 135

Query: 133 PSPPPAPPTSSPAAPPKAPAKPPT 157
           P   P   TS    P      P T
Sbjct: 136 PVGSPQAATSGILQPSSKKNNPAT 156

BLAST of MS004866 vs. TAIR 10
Match: AT2G31050.1 (Cupredoxin superfamily protein )

HSP 1 Score: 77.8 bits (190), Expect = 1.3e-14
Identity = 59/151 (39.07%), Postives = 87/151 (57.62%), Query Frame = 0

Query: 27  YTVGDSGGWEI-GTDFKSWASSKKFVVGDVLIFEYSAN-HDVLEVNEADFSSCSASNPIE 86
           + VGDS GW I   ++++WAS+  F VGD L+F+Y+ + HDV EV   D+  C  S P+ 
Sbjct: 29  HKVGDSDGWTIMSVNYETWASTITFQVGDSLVFKYNKDFHDVTEVTHNDYEMCEPSKPLA 88

Query: 87  KHTDGSTAIGLSTAGKRFFICGTPGHCSTGMKIEIDTLGTSSPPPEATPSPPPAPPTSSP 146
           ++  GS  + L+  G + FICG PGHC  G K++I  L  +S  P A P P P  P SS 
Sbjct: 89  RYETGSDIVILTKPGLQHFICGFPGHCDMGQKLQIHVL-PASLGPVAAPVPGPVRPPSSF 148

Query: 147 AAPPKAPAKPPTKSPLAPPPDSHVSPPAPPP 176
           ++P ++P     +SP+   P  +   P+P P
Sbjct: 149 SSPSQSPL---AESPVNHAPVQYQMGPSPAP 175

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140290.18.9e-11497.50blue copper protein-like [Momordica charantia][more]
XP_038900873.18.7e-7771.09blue copper protein-like [Benincasa hispida][more]
XP_022948200.11.5e-7367.70blue copper protein-like isoform X3 [Cucurbita moschata][more]
XP_022948201.12.6e-7369.80blue copper protein-like isoform X4 [Cucurbita moschata][more]
XP_022948199.11.7e-7267.70blue copper protein-like isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
O805176.6e-1937.57Uclacyanin-2 OS=Arabidopsis thaliana OX=3702 GN=At2g44790 PE=2 SV=1[more]
O820811.5e-1835.94Uclacyanin 1 OS=Arabidopsis thaliana OX=3702 GN=UCC1 PE=1 SV=1[more]
Q410014.3e-1842.86Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1[more]
P807284.3e-1845.45Mavicyanin OS=Cucurbita pepo OX=3663 PE=1 SV=1[more]
P003028.0e-1741.58Stellacyanin OS=Toxicodendron vernicifluum OX=4013 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CEP44.3e-11497.50blue copper protein-like OS=Momordica charantia OX=3673 GN=LOC111010993 PE=4 SV=... [more]
A0A6J1G9467.4e-7467.70blue copper protein-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC11145184... [more]
A0A6J1G8L41.3e-7369.80blue copper protein-like isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC11145184... [more]
A0A6J1G9638.2e-7367.70blue copper protein-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11145184... [more]
A0A6J1G8Q51.1e-7265.43blue copper protein-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11145184... [more]
Match NameE-valueIdentityDescription
AT3G60270.13.1e-2443.23Cupredoxin superfamily protein [more]
AT2G44790.14.7e-2037.57uclacyanin 2 [more]
AT2G32300.11.0e-1935.94uclacyanin 1 [more]
AT5G07475.12.6e-1836.81Cupredoxin superfamily protein [more]
AT2G31050.11.3e-1439.07Cupredoxin superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR01217PRICHEXTENSNcoord: 121..133
score: 43.08
coord: 162..174
score: 36.92
coord: 139..155
score: 41.18
coord: 175..196
score: 38.18
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 128..210
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 119..212
NoneNo IPR availablePANTHERPTHR33021:SF416BLUE COPPER PROTEIN-LIKEcoord: 6..236
NoneNo IPR availableCDDcd04216Phytocyanincoord: 25..120
e-value: 8.30117E-50
score: 156.266
IPR003245Phytocyanin domainPFAMPF02298Cu_bind_likecoord: 35..115
e-value: 1.8E-27
score: 95.2
IPR003245Phytocyanin domainPROSITEPS51485PHYTOCYANINcoord: 25..123
score: 39.333687
IPR008972CupredoxinGENE3D2.60.40.420coord: 23..126
e-value: 2.7E-35
score: 122.7
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 23..121
IPR039391PhytocyaninPANTHERPTHR33021BLUE COPPER PROTEINcoord: 6..236

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS004866.1MS004866.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0009055 electron transfer activity