MS004854 (gene) Bitter gourd (TR) v1

Overview


Name	MS004854
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Calcium-binding EF-hand protein
Location	scaffold176: 985510 .. 985845 (+)
RNA-Seq Expression	MS004854
Synteny	MS004854

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCACGAAAGGGTGGCCATTTCCTTGATTTACTACCTGTAATGGCCGAGAAACTGGGGGGAGAGGGGCTGATAAAGGAGCTGTGCAATGGGTTTCAGTTGCTGATGGACAAAGAGAAGGGGGTGATCACGCTGGAGAGCCTGAGGAGGAACTCTTCGCTGCTGGGTCTGCACAACATGGCAGAGGACGAGCTGGCCAGCATGATGAGGGAGGGCGACCTCGACGCCGACGGTGCCTTGAACCAGATGGAGTTTTGCGTTTTGATGTTCAGATTAAGCCCTCAGTTAATGGAACACTCTCTGTATTTGCTGCAGCACGCTTTAGAAGACGAGTTT

mRNA sequence

ATGGCACGAAAGGGTGGCCATTTCCTTGATTTACTACCTGTAATGGCCGAGAAACTGGGGGGAGAGGGGCTGATAAAGGAGCTGTGCAATGGGTTTCAGTTGCTGATGGACAAAGAGAAGGGGGTGATCACGCTGGAGAGCCTGAGGAGGAACTCTTCGCTGCTGGGTCTGCACAACATGGCAGAGGACGAGCTGGCCAGCATGATGAGGGAGGGCGACCTCGACGCCGACGGTGCCTTGAACCAGATGGAGTTTTGCGTTTTGATGTTCAGATTAAGCCCTCAGTTAATGGAACACTCTCTGTATTTGCTGCAGCACGCTTTAGAAGACGAGTTT

Coding sequence (CDS)

ATGGCACGAAAGGGTGGCCATTTCCTTGATTTACTACCTGTAATGGCCGAGAAACTGGGGGGAGAGGGGCTGATAAAGGAGCTGTGCAATGGGTTTCAGTTGCTGATGGACAAAGAGAAGGGGGTGATCACGCTGGAGAGCCTGAGGAGGAACTCTTCGCTGCTGGGTCTGCACAACATGGCAGAGGACGAGCTGGCCAGCATGATGAGGGAGGGCGACCTCGACGCCGACGGTGCCTTGAACCAGATGGAGTTTTGCGTTTTGATGTTCAGATTAAGCCCTCAGTTAATGGAACACTCTCTGTATTTGCTGCAGCACGCTTTAGAAGACGAGTTT

Protein sequence

MARKGGHFLDLLPVMAEKLGGEGLIKELCNGFQLLMDKEKGVITLESLRRNSSLLGLHNMAEDELASMMREGDLDADGALNQMEFCVLMFRLSPQLMEHSLYLLQHALEDEF

Homology

[Close]

BLAST of MS004854 vs. NCBI nr
Match: XP_004140055.1 (calcium-binding protein KRP1 [Cucumis sativus] >KGN46674.1 hypothetical protein Csa_020739 [Cucumis sativus])

HSP 1 Score: 184.1 bits (466), Expect = 6.6e-43
Identity = 94/113 (83.19%), Postives = 104/113 (92.04%), Query Frame = 0


Query: 1   MARKGG-HFLDLLPVMAEKLGGEGLIKELCNGFQLLMDKEKGVITLESLRRNSSLLGLHN 60
           M++KGG +F DLLPVMAEKLGGEGLIKELCNGF++LMDKEKGVITLESLRRNS LLG+ +
Sbjct: 1   MSKKGGANFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGVITLESLRRNSWLLGVRD 60

Query: 61  MAEDELASMMREGDLDADGALNQMEFCVLMFRLSPQLMEHSLYLLQHALEDEF 113
           MAE+EL SMMREGDLD DGALNQMEFCVLMFRLSP LM+HSLY LQ ALE+EF
Sbjct: 61  MAEEELLSMMREGDLDGDGALNQMEFCVLMFRLSPDLMQHSLYWLQLALENEF 113

[Close]

BLAST of MS004854 vs. NCBI nr
Match: KAG6570861.1 (Calcium-binding protein KRP1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 182.6 bits (462), Expect = 1.9e-42
Identity = 96/115 (83.48%), Postives = 100/115 (86.96%), Query Frame = 0


Query: 1   MARK---GGHFLDLLPVMAEKLGGEGLIKELCNGFQLLMDKEKGVITLESLRRNSSLLGL 60
           MARK   GG F DLLPVMAEKLGGEGLI ELCNGFQ+LMDKEKGVITLESLRRNSSLLGL
Sbjct: 1   MARKGGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSLLGL 60

Query: 61  HNMAEDELASMMREGDLDADGALNQMEFCVLMFRLSPQLMEHSLYLLQHALEDEF 113
            +M E+EL SMMREGDLD DGALNQMEFCVLMFRLSP+LME SLY L  ALE EF
Sbjct: 61  WDMGEEELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEF 115

[Close]

BLAST of MS004854 vs. NCBI nr
Match: XP_022944617.1 (calcium-binding protein PBP1-like [Cucurbita moschata] >KAG7010710.1 Calcium-binding protein PBP1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 181.4 bits (459), Expect = 4.3e-42
Identity = 96/118 (81.36%), Postives = 100/118 (84.75%), Query Frame = 0


Query: 1   MARK------GGHFLDLLPVMAEKLGGEGLIKELCNGFQLLMDKEKGVITLESLRRNSSL 60
           MARK      GG F DLLPVMAEKLGGEGLI ELCNGFQ+LMDKEKGVITLESLRRNSSL
Sbjct: 1   MARKGGGGGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSL 60

Query: 61  LGLHNMAEDELASMMREGDLDADGALNQMEFCVLMFRLSPQLMEHSLYLLQHALEDEF 113
           LGL +M E+EL SMMREGDLD DGALNQMEFCVLMFRLSP+LME SLY L  ALE EF
Sbjct: 61  LGLWDMGEEELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEF 118

[Close]

BLAST of MS004854 vs. NCBI nr
Match: XP_022986403.1 (calcium-binding protein PBP1-like [Cucurbita maxima])

HSP 1 Score: 181.0 bits (458), Expect = 5.6e-42
Identity = 96/119 (80.67%), Postives = 100/119 (84.03%), Query Frame = 0


Query: 1   MARK-------GGHFLDLLPVMAEKLGGEGLIKELCNGFQLLMDKEKGVITLESLRRNSS 60
           MARK       GG F DLLPVMAEKLGGEGLI ELCNGFQ+LMDKEKGVITLESLRRNSS
Sbjct: 1   MARKGGGGGGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSS 60

Query: 61  LLGLHNMAEDELASMMREGDLDADGALNQMEFCVLMFRLSPQLMEHSLYLLQHALEDEF 113
           LLGL +M E+EL SMMREGDLD DGALNQMEFCVLMFRLSP+LME SLY L  ALE EF
Sbjct: 61  LLGLWDMGEEELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEF 119

[Close]

BLAST of MS004854 vs. NCBI nr
Match: XP_023512862.1 (calcium-binding protein PBP1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 180.3 bits (456), Expect = 9.5e-42
Identity = 95/115 (82.61%), Postives = 99/115 (86.09%), Query Frame = 0


Query: 1   MARK---GGHFLDLLPVMAEKLGGEGLIKELCNGFQLLMDKEKGVITLESLRRNSSLLGL 60
           MARK   GG F DLLPVMAEKLGGEGLI ELCNGFQ+LMDKEKGVITLESLR NSSLLGL
Sbjct: 1   MARKGGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRTNSSLLGL 60

Query: 61  HNMAEDELASMMREGDLDADGALNQMEFCVLMFRLSPQLMEHSLYLLQHALEDEF 113
            +M E+EL SMMREGDLD DGALNQMEFCVLMFRLSP+LME SLY L  ALE EF
Sbjct: 61  WDMGEEELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEF 115

[Close]

BLAST of MS004854 vs. ExPASy Swiss-Prot
Match: O81831 (Calcium-binding protein KRP1 OS=Arabidopsis thaliana OX=3702 GN=KRP1 PE=2 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 3.2e-32
Identity = 69/107 (64.49%), Postives = 86/107 (80.37%), Query Frame = 0


Query: 7   HFLDLLPVMAEKLGGEGLIKELCNGFQLLMDKEKGVITLESLRRN-SSLLGLHNMAEDEL 66
           +F D LP MA  LGGEGLI ELCNGF+LLMD+EKGVIT ESLRRN +++LGL ++ ++++
Sbjct: 18  NFHDFLPTMAGNLGGEGLIGELCNGFELLMDREKGVITFESLRRNAAAVLGLGDLTDEDV 77

Query: 67  ASMMREGDLDADGALNQMEFCVLMFRLSPQLMEHSLYLLQHALEDEF 113
             M++EGD D DGALNQMEFCVLMFRLSP LME S  L+   +E+EF
Sbjct: 78  RCMIKEGDFDCDGALNQMEFCVLMFRLSPDLMEASRCLVTEVIEEEF 124

[Close]

BLAST of MS004854 vs. ExPASy Swiss-Prot
Match: Q9LSQ6 (Calcium-binding protein PBP1 OS=Arabidopsis thaliana OX=3702 GN=PBP1 PE=1 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 7.1e-32
Identity = 68/106 (64.15%), Postives = 85/106 (80.19%), Query Frame = 0


Query: 8   FLDLLPVMAEKLGGEGLIKELCNGFQLLMDKEKGVITLESLRRN-SSLLGLHNMAEDELA 67
           F D  P MA KLGGEGLI+E+C GF+LLMDK+KGVIT ESLRRN S++LGL ++ +D++ 
Sbjct: 19  FQDFFPTMAGKLGGEGLIEEICKGFELLMDKDKGVITFESLRRNASTVLGLGDLTDDDVR 78

Query: 68  SMMREGDLDADGALNQMEFCVLMFRLSPQLMEHSLYLLQHALEDEF 113
            M+ EGD D DGALNQMEFCVLMFRLSP+LME S  ++   +E+EF
Sbjct: 79  YMINEGDFDRDGALNQMEFCVLMFRLSPELMEASRCVVTEVIEEEF 124

[Close]

BLAST of MS004854 vs. ExPASy Swiss-Prot
Match: Q9ZPX9 (Calcium-binding protein KIC OS=Arabidopsis thaliana OX=3702 GN=KIC PE=1 SV=2)

HSP 1 Score: 119.8 bits (299), Expect = 2.0e-26
Identity = 59/102 (57.84%), Postives = 75/102 (73.53%), Query Frame = 0


Query: 10  DLLPVMAEKLGGEGLIKELCNGFQLLMDKEKGVITLESLRRNSSLLGLHNMAEDELASMM 69
           D+LPVMAEK+  E  + ELC GF LL D E+ +IT ESLRRNS +LG+  M++++   M+
Sbjct: 24  DMLPVMAEKMDVEEFVSELCKGFSLLADPERHLITAESLRRNSGILGIEGMSKEDAQGMV 83

Query: 70  REGDLDADGALNQMEFCVLMFRLSPQLMEHSLYLLQHALEDE 112
           REGDLD DGALNQ EFCVLM RLSP++ME +   L+ AL  E
Sbjct: 84  REGDLDGDGALNQTEFCVLMVRLSPEMMEDAETWLEKALTQE 125

[Close]

BLAST of MS004854 vs. ExPASy Swiss-Prot
Match: Q2TBN3 (Centrin-2 OS=Bos taurus OX=9913 GN=CETN2 PE=2 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 4.2e-08
Identity = 31/87 (35.63%), Postives = 52/87 (59.77%), Query Frame = 0


Query: 7   HFLDLLPVMAEKLGGEGLIKELCNGFQLLMDKEKGVITLESLRRNSSLLGLHNMAEDELA 66
           +F D L VM +K+  +   +E+   F+L  D E G I+ ++L+R +  LG  N++++EL 
Sbjct: 85  NFSDFLTVMTQKMSEKDTKEEILKAFKLFDDDETGKISFKNLKRVAKELG-ENLSDEELQ 144

Query: 67  SMMREGDLDADGALNQMEFCVLMFRLS 94
            M+ E D D DG +N+ EF  +M + S
Sbjct: 145 EMIDEADRDGDGEVNEQEFLRIMKKTS 170

[Close]

BLAST of MS004854 vs. ExPASy Swiss-Prot
Match: Q9R1K9 (Centrin-2 OS=Mus musculus OX=10090 GN=Cetn2 PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 5.5e-08
Identity = 31/87 (35.63%), Postives = 51/87 (58.62%), Query Frame = 0


Query: 7   HFLDLLPVMAEKLGGEGLIKELCNGFQLLMDKEKGVITLESLRRNSSLLGLHNMAEDELA 66
           +F D L VM +K+  +   +E+   F+L  D E G I+ ++L+R +  LG  N+ ++EL 
Sbjct: 85  NFSDFLTVMTQKMSEKDTKEEILKAFKLFDDDETGKISFKNLKRVAKELG-ENLTDEELQ 144

Query: 67  SMMREGDLDADGALNQMEFCVLMFRLS 94
            M+ E D D DG +N+ EF  +M + S
Sbjct: 145 EMIDEADRDGDGEVNEQEFLRIMKKTS 170

[Close]

BLAST of MS004854 vs. ExPASy TrEMBL
Match: A0A0A0KCV2 (EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G120930 PE=4 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 3.2e-43
Identity = 94/113 (83.19%), Postives = 104/113 (92.04%), Query Frame = 0


Query: 1   MARKGG-HFLDLLPVMAEKLGGEGLIKELCNGFQLLMDKEKGVITLESLRRNSSLLGLHN 60
           M++KGG +F DLLPVMAEKLGGEGLIKELCNGF++LMDKEKGVITLESLRRNS LLG+ +
Sbjct: 1   MSKKGGANFQDLLPVMAEKLGGEGLIKELCNGFEVLMDKEKGVITLESLRRNSWLLGVRD 60

Query: 61  MAEDELASMMREGDLDADGALNQMEFCVLMFRLSPQLMEHSLYLLQHALEDEF 113
           MAE+EL SMMREGDLD DGALNQMEFCVLMFRLSP LM+HSLY LQ ALE+EF
Sbjct: 61  MAEEELLSMMREGDLDGDGALNQMEFCVLMFRLSPDLMQHSLYWLQLALENEF 113

[Close]

BLAST of MS004854 vs. ExPASy TrEMBL
Match: A0A6J1FW37 (calcium-binding protein PBP1-like OS=Cucurbita moschata OX=3662 GN=LOC111449023 PE=4 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 2.1e-42
Identity = 96/118 (81.36%), Postives = 100/118 (84.75%), Query Frame = 0


Query: 1   MARK------GGHFLDLLPVMAEKLGGEGLIKELCNGFQLLMDKEKGVITLESLRRNSSL 60
           MARK      GG F DLLPVMAEKLGGEGLI ELCNGFQ+LMDKEKGVITLESLRRNSSL
Sbjct: 1   MARKGGGGGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSSL 60

Query: 61  LGLHNMAEDELASMMREGDLDADGALNQMEFCVLMFRLSPQLMEHSLYLLQHALEDEF 113
           LGL +M E+EL SMMREGDLD DGALNQMEFCVLMFRLSP+LME SLY L  ALE EF
Sbjct: 61  LGLWDMGEEELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEF 118

[Close]

BLAST of MS004854 vs. ExPASy TrEMBL
Match: A0A6J1JGE3 (calcium-binding protein PBP1-like OS=Cucurbita maxima OX=3661 GN=LOC111484150 PE=4 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 2.7e-42
Identity = 96/119 (80.67%), Postives = 100/119 (84.03%), Query Frame = 0


Query: 1   MARK-------GGHFLDLLPVMAEKLGGEGLIKELCNGFQLLMDKEKGVITLESLRRNSS 60
           MARK       GG F DLLPVMAEKLGGEGLI ELCNGFQ+LMDKEKGVITLESLRRNSS
Sbjct: 1   MARKGGGGGGGGGGFHDLLPVMAEKLGGEGLINELCNGFQMLMDKEKGVITLESLRRNSS 60

Query: 61  LLGLHNMAEDELASMMREGDLDADGALNQMEFCVLMFRLSPQLMEHSLYLLQHALEDEF 113
           LLGL +M E+EL SMMREGDLD DGALNQMEFCVLMFRLSP+LME SLY L  ALE EF
Sbjct: 61  LLGLWDMGEEELVSMMREGDLDGDGALNQMEFCVLMFRLSPELMEESLYWLDDALEKEF 119

[Close]

BLAST of MS004854 vs. ExPASy TrEMBL
Match: A0A6J1HZZ8 (calcium-binding protein PBP1-like OS=Cucurbita maxima OX=3661 GN=LOC111469641 PE=4 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 3.0e-41
Identity = 90/112 (80.36%), Postives = 96/112 (85.71%), Query Frame = 0


Query: 1   MARKGGHFLDLLPVMAEKLGGEGLIKELCNGFQLLMDKEKGVITLESLRRNSSLLGLHNM 60
           MARK   F DLLPVMAE+LGGEGLI ELCNGFQLLMDKEKGVITL+SLR NSSLLGL +M
Sbjct: 1   MARKSAAFHDLLPVMAEQLGGEGLINELCNGFQLLMDKEKGVITLDSLRLNSSLLGLRDM 60

Query: 61  AEDELASMMREGDLDADGALNQMEFCVLMFRLSPQLMEHSLYLLQHALEDEF 113
            E EL SMM EGDLD DGALNQMEFCVLMFRLSP+LM+HSLY LQHA   +F
Sbjct: 61  GEAELLSMMTEGDLDGDGALNQMEFCVLMFRLSPELMQHSLYCLQHASHHQF 112

[Close]

BLAST of MS004854 vs. ExPASy TrEMBL
Match: A0A6J1G6H5 (calcium-binding protein PBP1-like OS=Cucurbita moschata OX=3662 GN=LOC111451279 PE=4 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 1.9e-40
Identity = 89/111 (80.18%), Postives = 95/111 (85.59%), Query Frame = 0


Query: 1   MARKGGHFLDLLPVMAEKLGGEGLIKELCNGFQLLMDKEKGVITLESLRRNSSLLGLHNM 60
           MARK   F DLLPVMAE+LGGEGLI ELCNGFQLLMDKEKGVITL+SLR NSSLLGL +M
Sbjct: 1   MARKSAAFHDLLPVMAEQLGGEGLINELCNGFQLLMDKEKGVITLDSLRLNSSLLGLRDM 60

Query: 61  AEDELASMMREGDLDADGALNQMEFCVLMFRLSPQLMEHSLYLLQHALEDE 112
            E EL SMM EGDLD DGALNQMEFCVLMFRLSP+LMEHSL  LQHA  ++
Sbjct: 61  GEAELLSMMTEGDLDGDGALNQMEFCVLMFRLSPELMEHSLSFLQHAFHNQ 111

[Close]

BLAST of MS004854 vs. TAIR 10
Match: AT4G27280.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 139.0 bits (349), Expect = 2.3e-33
Identity = 69/107 (64.49%), Postives = 86/107 (80.37%), Query Frame = 0


Query: 7   HFLDLLPVMAEKLGGEGLIKELCNGFQLLMDKEKGVITLESLRRN-SSLLGLHNMAEDEL 66
           +F D LP MA  LGGEGLI ELCNGF+LLMD+EKGVIT ESLRRN +++LGL ++ ++++
Sbjct: 18  NFHDFLPTMAGNLGGEGLIGELCNGFELLMDREKGVITFESLRRNAAAVLGLGDLTDEDV 77

Query: 67  ASMMREGDLDADGALNQMEFCVLMFRLSPQLMEHSLYLLQHALEDEF 113
             M++EGD D DGALNQMEFCVLMFRLSP LME S  L+   +E+EF
Sbjct: 78  RCMIKEGDFDCDGALNQMEFCVLMFRLSPDLMEASRCLVTEVIEEEF 124

[Close]

BLAST of MS004854 vs. TAIR 10
Match: AT5G54490.1 (pinoid-binding protein 1 )

HSP 1 Score: 137.9 bits (346), Expect = 5.0e-33
Identity = 68/106 (64.15%), Postives = 85/106 (80.19%), Query Frame = 0


Query: 8   FLDLLPVMAEKLGGEGLIKELCNGFQLLMDKEKGVITLESLRRN-SSLLGLHNMAEDELA 67
           F D  P MA KLGGEGLI+E+C GF+LLMDK+KGVIT ESLRRN S++LGL ++ +D++ 
Sbjct: 19  FQDFFPTMAGKLGGEGLIEEICKGFELLMDKDKGVITFESLRRNASTVLGLGDLTDDDVR 78

Query: 68  SMMREGDLDADGALNQMEFCVLMFRLSPQLMEHSLYLLQHALEDEF 113
            M+ EGD D DGALNQMEFCVLMFRLSP+LME S  ++   +E+EF
Sbjct: 79  YMINEGDFDRDGALNQMEFCVLMFRLSPELMEASRCVVTEVIEEEF 124

[Close]

BLAST of MS004854 vs. TAIR 10
Match: AT2G46600.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 119.8 bits (299), Expect = 1.4e-27
Identity = 59/102 (57.84%), Postives = 75/102 (73.53%), Query Frame = 0


Query: 10  DLLPVMAEKLGGEGLIKELCNGFQLLMDKEKGVITLESLRRNSSLLGLHNMAEDELASMM 69
           D+LPVMAEK+  E  + ELC GF LL D E+ +IT ESLRRNS +LG+  M++++   M+
Sbjct: 24  DMLPVMAEKMDVEEFVSELCKGFSLLADPERHLITAESLRRNSGILGIEGMSKEDAQGMV 83

Query: 70  REGDLDADGALNQMEFCVLMFRLSPQLMEHSLYLLQHALEDE 112
           REGDLD DGALNQ EFCVLM RLSP++ME +   L+ AL  E
Sbjct: 84  REGDLDGDGALNQTEFCVLMVRLSPEMMEDAETWLEKALTQE 125

[Close]

BLAST of MS004854 vs. TAIR 10
Match: AT1G66410.1 (calmodulin 4 )

HSP 1 Score: 45.4 bits (106), Expect = 3.4e-05
Identity = 26/82 (31.71%), Postives = 44/82 (53.66%), Query Frame = 0


Query: 8   FLDLLPVMAEKLGGEGLIKELCNGFQLLMDKEKGVITLESLRRNSSLLGLHNMAEDELAS 67
           F + L +MA+K+      +EL   F++    + G I+   LR   + LG   + ++E+  
Sbjct: 66  FPEFLNLMAKKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLG-EKLTDEEVEE 125

Query: 68  MMREGDLDADGALNQMEFCVLM 90
           M+RE D+D DG +N  EF  +M
Sbjct: 126 MIREADVDGDGQINYEEFVKIM 146

[Close]

BLAST of MS004854 vs. TAIR 10
Match: AT1G66410.2 (calmodulin 4 )

HSP 1 Score: 45.4 bits (106), Expect = 3.4e-05
Identity = 26/82 (31.71%), Postives = 44/82 (53.66%), Query Frame = 0


Query: 8   FLDLLPVMAEKLGGEGLIKELCNGFQLLMDKEKGVITLESLRRNSSLLGLHNMAEDELAS 67
           F + L +MA+K+      +EL   F++    + G I+   LR   + LG   + ++E+  
Sbjct: 76  FPEFLNLMAKKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLG-EKLTDEEVEE 135

Query: 68  MMREGDLDADGALNQMEFCVLM 90
           M+RE D+D DG +N  EF  +M
Sbjct: 136 MIREADVDGDGQINYEEFVKIM 156

The following BLAST results are available for this feature:

BLAST of MS004854 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004140055.1	6.6e-43	83.19	calcium-binding protein KRP1 [Cucumis sativus] >KGN46674.1 hypothetical protein ...	[more]
KAG6570861.1	1.9e-42	83.48	Calcium-binding protein KRP1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022944617.1	4.3e-42	81.36	calcium-binding protein PBP1-like [Cucurbita moschata] >KAG7010710.1 Calcium-bin...	[more]
XP_022986403.1	5.6e-42	80.67	calcium-binding protein PBP1-like [Cucurbita maxima]	[more]
XP_023512862.1	9.5e-42	82.61	calcium-binding protein PBP1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS004854 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81831	3.2e-32	64.49	Calcium-binding protein KRP1 OS=Arabidopsis thaliana OX=3702 GN=KRP1 PE=2 SV=1	[more]
Q9LSQ6	7.1e-32	64.15	Calcium-binding protein PBP1 OS=Arabidopsis thaliana OX=3702 GN=PBP1 PE=1 SV=1	[more]
Q9ZPX9	2.0e-26	57.84	Calcium-binding protein KIC OS=Arabidopsis thaliana OX=3702 GN=KIC PE=1 SV=2	[more]
Q2TBN3	4.2e-08	35.63	Centrin-2 OS=Bos taurus OX=9913 GN=CETN2 PE=2 SV=1	[more]
Q9R1K9	5.5e-08	35.63	Centrin-2 OS=Mus musculus OX=10090 GN=Cetn2 PE=1 SV=1	[more]

BLAST of MS004854 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KCV2	3.2e-43	83.19	EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G120930 PE=...	[more]
A0A6J1FW37	2.1e-42	81.36	calcium-binding protein PBP1-like OS=Cucurbita moschata OX=3662 GN=LOC111449023 ...	[more]
A0A6J1JGE3	2.7e-42	80.67	calcium-binding protein PBP1-like OS=Cucurbita maxima OX=3661 GN=LOC111484150 PE...	[more]
A0A6J1HZZ8	3.0e-41	80.36	calcium-binding protein PBP1-like OS=Cucurbita maxima OX=3661 GN=LOC111469641 PE...	[more]
A0A6J1G6H5	1.9e-40	80.18	calcium-binding protein PBP1-like OS=Cucurbita moschata OX=3662 GN=LOC111451279 ...	[more]

BLAST of MS004854 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27280.1	2.3e-33	64.49	Calcium-binding EF-hand family protein	[more]
AT5G54490.1	5.0e-33	64.15	pinoid-binding protein 1	[more]
AT2G46600.1	1.4e-27	57.84	Calcium-binding EF-hand family protein	[more]
AT1G66410.1	3.4e-05	31.71	calmodulin 4	[more]
AT1G66410.2	3.4e-05	31.71	calmodulin 4	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.238.10		coord: 6..112 e-value: 2.6E-20 score: 74.4
None	No IPR available	PANTHER	PTHR47319:SF8	CALCIUM-BINDING PROTEIN PBP1-LIKE	coord: 3..112
IPR002048	EF-hand domain	PFAM	PF13833	EF-hand_8	coord: 39..91 e-value: 8.5E-18 score: 63.9
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 60..95 score: 9.464338
IPR044205	Calcium-binding protein KIC/PBP1/KRP1	PANTHER	PTHR47319	CALCIUM-BINDING PROTEIN KIC	coord: 3..112
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 73..85
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 23..99

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS004854.1	MS004854.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding