MS004847 (gene) Bitter gourd (TR) v1

BLAST of MS004847 vs. NCBI nr
Match: XP_022947263.1 (uncharacterized GPI-anchored protein At4g28100-like [Cucurbita moschata])

HSP 1 Score: 466.8 bits (1200), Expect = 1.4e-127
Identity = 243/314 (77.39%), Postives = 265/314 (84.39%), Query Frame = 0

Query: 4   ILASSILLLSTLFCISAADFQVESVDGENQVLLAGEYPLLNSVPAFPVEIETQACRLDLS 63
           +++S +++LSTLF +S A      V GE            +SVPA PVE ETQ CRLDLS
Sbjct: 112 VVSSILMILSTLFQVSVA-----GVTGE------------SSVPAIPVEAETQVCRLDLS 171

Query: 64  DELFGGVRQACNGNLDRSRCCPVLAAWLFAAHARSALEVSGPAASGETDLPLMPDDSQKC 123
           DELFGGV QACNGNLDRSRCCPVLAAWLFAAHARSALEVS PA + E DLPLMPDDSQKC
Sbjct: 172 DELFGGVSQACNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKC 231

Query: 124 VESLQSSLVRRNIRIPQPNASCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASAAPTAA 183
           VESLQ+SLV+RNIRIPQPN+SCDAVLCFCGIRLHQISSLSCPAAFNLSGF NA+  PT+A
Sbjct: 232 VESLQNSLVKRNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNAT--PTSA 291

Query: 184 VRDLEKNCRNASYSGCTKCLGALHKVKGAPSRDSSTTERGTKMFSRDCQLMGLTWLLARN 243
           VRDLEKNCRNASYSGCTKCLGAL KVKGA  RD+STTER TKMF+RDCQLMGLTWLLARN
Sbjct: 292 VRDLEKNCRNASYSGCTKCLGALQKVKGA--RDTSTTERATKMFNRDCQLMGLTWLLARN 351

Query: 244 KTAYIPTVSAVLRAIMYSAHPPRDSKCSPDQENMPLAVDSLQFEEAQSCSSLLSVFGFPF 303
           KTAYIPTVSAVLRAIMYSAHPP DSKCSPDQENMPLAVDSLQFE  QS +S  S F +PF
Sbjct: 352 KTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPSTFMYPF 404

Query: 304 LPLFVLPFVFVLVY 318
           LPL  +PF++ L +
Sbjct: 412 LPLTAVPFIWFLTH 404

BLAST of MS004847 vs. NCBI nr
Match: KAG7034939.1 (putative GPI-anchored protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 466.8 bits (1200), Expect = 1.4e-127
Identity = 242/309 (78.32%), Postives = 262/309 (84.79%), Query Frame = 0

Query: 4   ILASSILLLSTLFCISAADFQVESVDGENQVLLAGEYPLLNSVPAFPVEIETQACRLDLS 63
           +++S +++LSTLF +S A      V GE            +SVPA PVE ETQ CRLDLS
Sbjct: 7   VVSSILMILSTLFQVSVA-----GVTGE------------SSVPAIPVEAETQVCRLDLS 66

Query: 64  DELFGGVRQACNGNLDRSRCCPVLAAWLFAAHARSALEVSGPAASGETDLPLMPDDSQKC 123
           DELFGGV QACNGNLDRSRCCPVLAAWLFAAHARSALEVS PA + E DLPLMPDDSQKC
Sbjct: 67  DELFGGVSQACNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKC 126

Query: 124 VESLQSSLVRRNIRIPQPNASCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASAAPTAA 183
           VESLQ+SLV+RNIRIPQPN+SCDAVLCFCGIRLHQISSLSCPAAFNLSGF NA+  PT+A
Sbjct: 127 VESLQNSLVKRNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNAT--PTSA 186

Query: 184 VRDLEKNCRNASYSGCTKCLGALHKVKGAPSRDSSTTERGTKMFSRDCQLMGLTWLLARN 243
           VRDLEKNCRNASYSGCTKCLGAL KVKGA  RD+STTER TKMF+RDCQLMGLTWLLARN
Sbjct: 187 VRDLEKNCRNASYSGCTKCLGALQKVKGA--RDTSTTERATKMFNRDCQLMGLTWLLARN 246

Query: 244 KTAYIPTVSAVLRAIMYSAHPPRDSKCSPDQENMPLAVDSLQFEEAQSCSSLLSVFGFPF 303
           KTAYIPTVSAVLRAIMYSAHPP DSKCSPDQENMPLAVDSLQFE  QS +S  S F +PF
Sbjct: 247 KTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPSTFMYPF 294

Query: 304 LPLFVLPFV 313
           LPL  +PF+
Sbjct: 307 LPLTAVPFI 294

BLAST of MS004847 vs. NCBI nr
Match: XP_023532424.1 (uncharacterized GPI-anchored protein At4g28100-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 462.6 bits (1189), Expect = 2.7e-126
Identity = 241/314 (76.75%), Postives = 263/314 (83.76%), Query Frame = 0

Query: 4   ILASSILLLSTLFCISAADFQVESVDGENQVLLAGEYPLLNSVPAFPVEIETQACRLDLS 63
           +++S +++LSTLF +S A      V GE            +SVPA PVE ETQ CRLDLS
Sbjct: 7   VVSSILMILSTLFQVSVA-----GVTGE------------SSVPAIPVEAETQVCRLDLS 66

Query: 64  DELFGGVRQACNGNLDRSRCCPVLAAWLFAAHARSALEVSGPAASGETDLPLMPDDSQKC 123
           DELFGGV QACNGNLDRSRCCPVLAAWLFAAHARSALEVS PA + E DLPLMPDDSQKC
Sbjct: 67  DELFGGVSQACNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKC 126

Query: 124 VESLQSSLVRRNIRIPQPNASCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASAAPTAA 183
           VESLQ+SLV+RNIRIPQPN+SCDAVLCFCGIRLHQISSLSCPAAFNLSGF NA+  PT A
Sbjct: 127 VESLQNSLVKRNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNAT--PTPA 186

Query: 184 VRDLEKNCRNASYSGCTKCLGALHKVKGAPSRDSSTTERGTKMFSRDCQLMGLTWLLARN 243
           VRDLEKNCRNASYSGCTKCLGAL KVKGA  RD+STTER TKMF+RDCQLMGLTWLLARN
Sbjct: 187 VRDLEKNCRNASYSGCTKCLGALQKVKGA--RDTSTTERATKMFNRDCQLMGLTWLLARN 246

Query: 244 KTAYIPTVSAVLRAIMYSAHPPRDSKCSPDQENMPLAVDSLQFEEAQSCSSLLSVFGFPF 303
           KTAYIPTVSAVLRAIMYSAHPP DSKCSPDQENMPLAVDSLQ +  QS +S  S F +PF
Sbjct: 247 KTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQLDNTQSSTSSPSTFMYPF 299

Query: 304 LPLFVLPFVFVLVY 318
           LPL  +PF++ L +
Sbjct: 307 LPLTAVPFIWFLTH 299

BLAST of MS004847 vs. NCBI nr
Match: KAG6604823.1 (putative GPI-anchored protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 462.2 bits (1188), Expect = 3.5e-126
Identity = 232/274 (84.67%), Postives = 247/274 (90.15%), Query Frame = 0

Query: 44  NSVPAFPVEIETQACRLDLSDELFGGVRQACNGNLDRSRCCPVLAAWLFAAHARSALEVS 103
           +SVPA PVE ETQ CRLDLSDELFGGV QACNGNLDRSRCCPVLAAWLFAAHARSALEVS
Sbjct: 150 SSVPAIPVEAETQVCRLDLSDELFGGVSQACNGNLDRSRCCPVLAAWLFAAHARSALEVS 209

Query: 104 GPAASGETDLPLMPDDSQKCVESLQSSLVRRNIRIPQPNASCDAVLCFCGIRLHQISSLS 163
            PA + E DLPLMPDDSQKCVESLQ+SLV+RNIRIPQPN+SCDAVLCFCGIRLHQISSLS
Sbjct: 210 APAPTAEIDLPLMPDDSQKCVESLQNSLVKRNIRIPQPNSSCDAVLCFCGIRLHQISSLS 269

Query: 164 CPAAFNLSGFQNASAAPTAAVRDLEKNCRNASYSGCTKCLGALHKVKGAPSRDSSTTERG 223
           CPAAFNLSGF NA+  PT+AVRDLEKNCRNASYSGCTKCLGAL KVKGA  RD+STTER 
Sbjct: 270 CPAAFNLSGFHNAT--PTSAVRDLEKNCRNASYSGCTKCLGALQKVKGA--RDTSTTERA 329

Query: 224 TKMFSRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYSAHPPRDSKCSPDQENMPLAVDS 283
           TKMF+RDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYSAHPP DSKCSPDQENMPLAVDS
Sbjct: 330 TKMFNRDCQLMGLTWLLARNKTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDS 389

Query: 284 LQFEEAQSCSSLLSVFGFPFLPLFVLPFVFVLVY 318
           LQFE  QS +S  S F +PFLPL  +PF++ L +
Sbjct: 390 LQFENTQSSTSSPSTFMYPFLPLTAVPFIWFLTH 419

BLAST of MS004847 vs. NCBI nr
Match: XP_022970732.1 (uncharacterized GPI-anchored protein At4g28100-like [Cucurbita maxima])

HSP 1 Score: 461.1 bits (1185), Expect = 7.9e-126
Identity = 241/314 (76.75%), Postives = 263/314 (83.76%), Query Frame = 0

Query: 4   ILASSILLLSTLFCISAADFQVESVDGENQVLLAGEYPLLNSVPAFPVEIETQACRLDLS 63
           +++S +++LSTLF +S A      V GE            +SVPA PVE ETQ CRLDLS
Sbjct: 7   VVSSILMILSTLFQVSVA-----GVTGE------------SSVPAIPVEAETQVCRLDLS 66

Query: 64  DELFGGVRQACNGNLDRSRCCPVLAAWLFAAHARSALEVSGPAASGETDLPLMPDDSQKC 123
           DELFGGV QACNGNLDRSRCCPVLAAWLFAAHARSALEVS PA + E DLPLMPDDSQKC
Sbjct: 67  DELFGGVSQACNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKC 126

Query: 124 VESLQSSLVRRNIRIPQPNASCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASAAPTAA 183
           VESLQ+SLV+RNIRIPQPN+SCDAVLCFCGIRLHQISSLSCPAAFNLSGF NA+  PT A
Sbjct: 127 VESLQNSLVKRNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNAT--PTPA 186

Query: 184 VRDLEKNCRNASYSGCTKCLGALHKVKGAPSRDSSTTERGTKMFSRDCQLMGLTWLLARN 243
           VRDLEKNCRNASYSGCTKCLGAL KVKGA  RD++TTER TKMF+RDCQLMGLTWLLARN
Sbjct: 187 VRDLEKNCRNASYSGCTKCLGALQKVKGA--RDTNTTERATKMFNRDCQLMGLTWLLARN 246

Query: 244 KTAYIPTVSAVLRAIMYSAHPPRDSKCSPDQENMPLAVDSLQFEEAQSCSSLLSVFGFPF 303
           KTAYIPTVSAVLRAIMYSAHPP DSKCSPDQENMPLAVDSLQFE  QS +S  S F +PF
Sbjct: 247 KTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPSTFMYPF 299

Query: 304 LPLFVLPFVFVLVY 318
           LPL  + F++ L +
Sbjct: 307 LPLTAVLFIWFLTH 299

BLAST of MS004847 vs. ExPASy Swiss-Prot
Match: Q9SUC9 (Uncharacterized GPI-anchored protein At4g28100 OS=Arabidopsis thaliana OX=3702 GN=At4g28100 PE=2 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 2.2e-107
Identity = 208/312 (66.67%), Postives = 244/312 (78.21%), Query Frame = 0

Query: 9   ILLLSTLFCISAADFQVESVDGENQVLLAGEYPLLNSVPAFPVEIETQACRLDLSDELFG 68
           +LL S LF    ++  VE V               N+VPAFPVE + Q+CRLDLS+ELFG
Sbjct: 10  LLLCSLLFSTVLSNLLVEPVQP-------------NTVPAFPVETQAQSCRLDLSNELFG 69

Query: 69  GVRQACNGNLDRSRCCPVLAAWLFAAHARSALEVSGPA---ASGETDLPLMPDDSQKCVE 128
           GV +AC  NLDRSRCCPVLAAWLFAAHARSAL++  PA    S + D P+ PDDSQKCV 
Sbjct: 70  GVNEACGRNLDRSRCCPVLAAWLFAAHARSALQLPAPAPTPESSDPDEPMKPDDSQKCVN 129

Query: 129 SLQSSLVRRNIRIPQPNASCDAVLCFCGIRLHQISSLSCPAAFNL-SGFQNASAAPTAAV 188
           +LQS+L+ + I+IPQPN+SCDA+LCFCGIRLHQISSLSCPAAFN+ SGF+NA+  PTAAV
Sbjct: 130 TLQSALLTKQIKIPQPNSSCDAILCFCGIRLHQISSLSCPAAFNVSSGFKNAT--PTAAV 189

Query: 189 RDLEKNCRNASYSGCTKCLGALHKVKGAPSRDSSTTERGTKMFSRDCQLMGLTWLLARNK 248
           ++LEK CRN+SYSGCT+CLGAL K+K       +TTERGTKM S+DCQLMGLTWLLARNK
Sbjct: 190 KNLEKECRNSSYSGCTRCLGALQKLKVRGGNKKTTTERGTKMMSKDCQLMGLTWLLARNK 249

Query: 249 TAYIPTVSAVLRAIMYSAHPPRDSKCSPDQENMPLAVDSLQFEEAQSCSSLLSVFG-FPF 308
           TAYIPTVSAVLRAIMYS HPP  +KCSPDQENMPLAVDSLQF+++ S SS L  FG  PF
Sbjct: 250 TAYIPTVSAVLRAIMYSPHPPHLNKCSPDQENMPLAVDSLQFQKSFSSSSHL--FGVLPF 304

Query: 309 LPLFVLPFVFVL 316
           LPL +  F+F+L
Sbjct: 310 LPLVLCIFLFLL 304

BLAST of MS004847 vs. ExPASy TrEMBL
Match: A0A6J1G647 (uncharacterized GPI-anchored protein At4g28100-like OS=Cucurbita moschata OX=3662 GN=LOC111451182 PE=4 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 7.0e-128
Identity = 243/314 (77.39%), Postives = 265/314 (84.39%), Query Frame = 0

Query: 4   ILASSILLLSTLFCISAADFQVESVDGENQVLLAGEYPLLNSVPAFPVEIETQACRLDLS 63
           +++S +++LSTLF +S A      V GE            +SVPA PVE ETQ CRLDLS
Sbjct: 112 VVSSILMILSTLFQVSVA-----GVTGE------------SSVPAIPVEAETQVCRLDLS 171

Query: 64  DELFGGVRQACNGNLDRSRCCPVLAAWLFAAHARSALEVSGPAASGETDLPLMPDDSQKC 123
           DELFGGV QACNGNLDRSRCCPVLAAWLFAAHARSALEVS PA + E DLPLMPDDSQKC
Sbjct: 172 DELFGGVSQACNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKC 231

Query: 124 VESLQSSLVRRNIRIPQPNASCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASAAPTAA 183
           VESLQ+SLV+RNIRIPQPN+SCDAVLCFCGIRLHQISSLSCPAAFNLSGF NA+  PT+A
Sbjct: 232 VESLQNSLVKRNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNAT--PTSA 291

Query: 184 VRDLEKNCRNASYSGCTKCLGALHKVKGAPSRDSSTTERGTKMFSRDCQLMGLTWLLARN 243
           VRDLEKNCRNASYSGCTKCLGAL KVKGA  RD+STTER TKMF+RDCQLMGLTWLLARN
Sbjct: 292 VRDLEKNCRNASYSGCTKCLGALQKVKGA--RDTSTTERATKMFNRDCQLMGLTWLLARN 351

Query: 244 KTAYIPTVSAVLRAIMYSAHPPRDSKCSPDQENMPLAVDSLQFEEAQSCSSLLSVFGFPF 303
           KTAYIPTVSAVLRAIMYSAHPP DSKCSPDQENMPLAVDSLQFE  QS +S  S F +PF
Sbjct: 352 KTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPSTFMYPF 404

Query: 304 LPLFVLPFVFVLVY 318
           LPL  +PF++ L +
Sbjct: 412 LPLTAVPFIWFLTH 404

BLAST of MS004847 vs. ExPASy TrEMBL
Match: A0A6J1HZY7 (uncharacterized GPI-anchored protein At4g28100-like OS=Cucurbita maxima OX=3661 GN=LOC111469629 PE=4 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 3.8e-126
Identity = 241/314 (76.75%), Postives = 263/314 (83.76%), Query Frame = 0

Query: 4   ILASSILLLSTLFCISAADFQVESVDGENQVLLAGEYPLLNSVPAFPVEIETQACRLDLS 63
           +++S +++LSTLF +S A      V GE            +SVPA PVE ETQ CRLDLS
Sbjct: 7   VVSSILMILSTLFQVSVA-----GVTGE------------SSVPAIPVEAETQVCRLDLS 66

Query: 64  DELFGGVRQACNGNLDRSRCCPVLAAWLFAAHARSALEVSGPAASGETDLPLMPDDSQKC 123
           DELFGGV QACNGNLDRSRCCPVLAAWLFAAHARSALEVS PA + E DLPLMPDDSQKC
Sbjct: 67  DELFGGVSQACNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPTAEIDLPLMPDDSQKC 126

Query: 124 VESLQSSLVRRNIRIPQPNASCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASAAPTAA 183
           VESLQ+SLV+RNIRIPQPN+SCDAVLCFCGIRLHQISSLSCPAAFNLSGF NA+  PT A
Sbjct: 127 VESLQNSLVKRNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFHNAT--PTPA 186

Query: 184 VRDLEKNCRNASYSGCTKCLGALHKVKGAPSRDSSTTERGTKMFSRDCQLMGLTWLLARN 243
           VRDLEKNCRNASYSGCTKCLGAL KVKGA  RD++TTER TKMF+RDCQLMGLTWLLARN
Sbjct: 187 VRDLEKNCRNASYSGCTKCLGALQKVKGA--RDTNTTERATKMFNRDCQLMGLTWLLARN 246

Query: 244 KTAYIPTVSAVLRAIMYSAHPPRDSKCSPDQENMPLAVDSLQFEEAQSCSSLLSVFGFPF 303
           KTAYIPTVSAVLRAIMYSAHPP DSKCSPDQENMPLAVDSLQFE  QS +S  S F +PF
Sbjct: 247 KTAYIPTVSAVLRAIMYSAHPPHDSKCSPDQENMPLAVDSLQFENTQSSTSSPSTFMYPF 299

Query: 304 LPLFVLPFVFVLVY 318
           LPL  + F++ L +
Sbjct: 307 LPLTAVLFIWFLTH 299

BLAST of MS004847 vs. ExPASy TrEMBL
Match: A0A0A0KAY3 (SPARK domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G121950 PE=4 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 1.1e-122
Identity = 246/315 (78.10%), Postives = 258/315 (81.90%), Query Frame = 0

Query: 1   MTPILASSILLLS-TLFCISAADFQVESVDGENQVLLAGEYPLLNSVPAFPVEIETQACR 60
           M  ILA + L++S TLF IS A            V + G+Y    SVPAFPVE E QACR
Sbjct: 1   MISILAIATLVISTTLFPISVA------------VSVRGQY----SVPAFPVETENQACR 60

Query: 61  LDLSDELFGGVRQACNGNLDRSRCCPVLAAWLFAAHARSALEVSGPAASGETDLPLMPDD 120
           LDLSDELFGGV QACNGNLDRSRCCPVLAAWLFAAHARSALEVS PA S E DLPLMPDD
Sbjct: 61  LDLSDELFGGVSQACNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDD 120

Query: 121 SQKCVESLQSSLVRRNIRIPQPNASCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASAA 180
           SQKCVESLQSSLVRRNIRIPQPN+SCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNAS  
Sbjct: 121 SQKCVESLQSSLVRRNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNAS-- 180

Query: 181 PTAAVRDLEKNCRNASYSGCTKCLGALHKVKGAPSRDSSTTERGTKMFSRDCQLMGLTWL 240
           PTAAVRDLE NCRNASYSGCTKCLGAL KVKGA   D STT R  KMF+RDCQLMGLTWL
Sbjct: 181 PTAAVRDLETNCRNASYSGCTKCLGALQKVKGA--GDRSTTARARKMFNRDCQLMGLTWL 240

Query: 241 LARNKTAYIPTVSAVLRAIMYSAHPPRDSKCSPDQENMPLAVDSLQFEEAQSC--SSLLS 300
           LARNKTAYIPTVSAVLRAIMYSAHPP +S CSPDQENMPLAVDSLQF++A S   S   S
Sbjct: 241 LARNKTAYIPTVSAVLRAIMYSAHPPHNSMCSPDQENMPLAVDSLQFDDAHSISHSQPSS 295

Query: 301 VFGFPFLPLFVLPFV 313
           +F F FL L + PFV
Sbjct: 301 IFIFSFLRLLLTPFV 295

BLAST of MS004847 vs. ExPASy TrEMBL
Match: A0A5D3B9U0 (Putative GPI-anchored protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold464G00450 PE=4 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 6.3e-121
Identity = 243/314 (77.39%), Postives = 256/314 (81.53%), Query Frame = 0

Query: 1   MTPILASSILLLS-TLFCISAADFQVESVDGENQVLLAGEYPLLNSVPAFPVEIETQACR 60
           M  ILA +  L+S TLF IS A            V++ G++    SVPAFPVE E QACR
Sbjct: 1   MISILAIATFLISTTLFPISVA------------VVVRGQF----SVPAFPVETENQACR 60

Query: 61  LDLSDELFGGVRQACNGNLDRSRCCPVLAAWLFAAHARSALEVSGPAASGETDLPLMPDD 120
           LDLSDELFGGV QACNGNLDRSRCCPVLAAWLFAAHARSALEVS PA S E DLPLMPDD
Sbjct: 61  LDLSDELFGGVSQACNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDD 120

Query: 121 SQKCVESLQSSLVRRNIRIPQPNASCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASAA 180
           SQKCVESLQSSLVRRNIRIPQPN+SCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNAS  
Sbjct: 121 SQKCVESLQSSLVRRNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNAS-- 180

Query: 181 PTAAVRDLEKNCRNASYSGCTKCLGALHKVKGAPSRDSSTTERGTKMFSRDCQLMGLTWL 240
           PT AVRDLE NCRNASYSGCTKCLGAL KVKGA   D STT R  KMF+RDCQLMGLTWL
Sbjct: 181 PTTAVRDLETNCRNASYSGCTKCLGALQKVKGA--GDRSTTARARKMFNRDCQLMGLTWL 240

Query: 241 LARNKTAYIPTVSAVLRAIMYSAHPPRDSKCSPDQENMPLAVDSLQFEEAQSCS-SLLSV 300
           LARNKTAYIPTVSAVLRAIMYSAH P +S CSPDQENMPLAVDSLQF++A S S S  S+
Sbjct: 241 LARNKTAYIPTVSAVLRAIMYSAHLPHNSMCSPDQENMPLAVDSLQFDDAHSISHSQPSI 294

Query: 301 FGFPFLPLFVLPFV 313
           F F F  L + PFV
Sbjct: 301 FMFSFFRLVLTPFV 294

BLAST of MS004847 vs. ExPASy TrEMBL
Match: A0A1S3BJT3 (uncharacterized GPI-anchored protein At4g28100-like OS=Cucumis melo OX=3656 GN=LOC103490480 PE=4 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 6.3e-121
Identity = 243/314 (77.39%), Postives = 256/314 (81.53%), Query Frame = 0

Query: 1   MTPILASSILLLS-TLFCISAADFQVESVDGENQVLLAGEYPLLNSVPAFPVEIETQACR 60
           M  ILA +  L+S TLF IS A            V++ G++    SVPAFPVE E QACR
Sbjct: 1   MISILAIATFLISTTLFPISVA------------VVVRGQF----SVPAFPVETENQACR 60

Query: 61  LDLSDELFGGVRQACNGNLDRSRCCPVLAAWLFAAHARSALEVSGPAASGETDLPLMPDD 120
           LDLSDELFGGV QACNGNLDRSRCCPVLAAWLFAAHARSALEVS PA S E DLPLMPDD
Sbjct: 61  LDLSDELFGGVSQACNGNLDRSRCCPVLAAWLFAAHARSALEVSAPAPSAEIDLPLMPDD 120

Query: 121 SQKCVESLQSSLVRRNIRIPQPNASCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNASAA 180
           SQKCVESLQSSLVRRNIRIPQPN+SCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNAS  
Sbjct: 121 SQKCVESLQSSLVRRNIRIPQPNSSCDAVLCFCGIRLHQISSLSCPAAFNLSGFQNAS-- 180

Query: 181 PTAAVRDLEKNCRNASYSGCTKCLGALHKVKGAPSRDSSTTERGTKMFSRDCQLMGLTWL 240
           PT AVRDLE NCRNASYSGCTKCLGAL KVKGA   D STT R  KMF+RDCQLMGLTWL
Sbjct: 181 PTTAVRDLETNCRNASYSGCTKCLGALQKVKGA--GDRSTTARARKMFNRDCQLMGLTWL 240

Query: 241 LARNKTAYIPTVSAVLRAIMYSAHPPRDSKCSPDQENMPLAVDSLQFEEAQSCS-SLLSV 300
           LARNKTAYIPTVSAVLRAIMYSAH P +S CSPDQENMPLAVDSLQF++A S S S  S+
Sbjct: 241 LARNKTAYIPTVSAVLRAIMYSAHLPHNSMCSPDQENMPLAVDSLQFDDAHSISHSQPSI 294

Query: 301 FGFPFLPLFVLPFV 313
           F F F  L + PFV
Sbjct: 301 FMFSFFRLVLTPFV 294

BLAST of MS004847 vs. TAIR 10
Match: AT4G28100.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: anchored to plasma membrane, anchored to membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G18050.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 390.2 bits (1001), Expect = 1.6e-108
Identity = 208/312 (66.67%), Postives = 244/312 (78.21%), Query Frame = 0

Query: 9   ILLLSTLFCISAADFQVESVDGENQVLLAGEYPLLNSVPAFPVEIETQACRLDLSDELFG 68
           +LL S LF    ++  VE V               N+VPAFPVE + Q+CRLDLS+ELFG
Sbjct: 10  LLLCSLLFSTVLSNLLVEPVQP-------------NTVPAFPVETQAQSCRLDLSNELFG 69

Query: 69  GVRQACNGNLDRSRCCPVLAAWLFAAHARSALEVSGPA---ASGETDLPLMPDDSQKCVE 128
           GV +AC  NLDRSRCCPVLAAWLFAAHARSAL++  PA    S + D P+ PDDSQKCV 
Sbjct: 70  GVNEACGRNLDRSRCCPVLAAWLFAAHARSALQLPAPAPTPESSDPDEPMKPDDSQKCVN 129

Query: 129 SLQSSLVRRNIRIPQPNASCDAVLCFCGIRLHQISSLSCPAAFNL-SGFQNASAAPTAAV 188
           +LQS+L+ + I+IPQPN+SCDA+LCFCGIRLHQISSLSCPAAFN+ SGF+NA+  PTAAV
Sbjct: 130 TLQSALLTKQIKIPQPNSSCDAILCFCGIRLHQISSLSCPAAFNVSSGFKNAT--PTAAV 189

Query: 189 RDLEKNCRNASYSGCTKCLGALHKVKGAPSRDSSTTERGTKMFSRDCQLMGLTWLLARNK 248
           ++LEK CRN+SYSGCT+CLGAL K+K       +TTERGTKM S+DCQLMGLTWLLARNK
Sbjct: 190 KNLEKECRNSSYSGCTRCLGALQKLKVRGGNKKTTTERGTKMMSKDCQLMGLTWLLARNK 249

Query: 249 TAYIPTVSAVLRAIMYSAHPPRDSKCSPDQENMPLAVDSLQFEEAQSCSSLLSVFG-FPF 308
           TAYIPTVSAVLRAIMYS HPP  +KCSPDQENMPLAVDSLQF+++ S SS L  FG  PF
Sbjct: 250 TAYIPTVSAVLRAIMYSPHPPHLNKCSPDQENMPLAVDSLQFQKSFSSSSHL--FGVLPF 304

Query: 309 LPLFVLPFVFVL 316
           LPL +  F+F+L
Sbjct: 310 LPLVLCIFLFLL 304

BLAST of MS004847 vs. TAIR 10
Match: AT3G18050.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G28100.1); Has 67 Blast hits to 66 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 67; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 198.7 bits (504), Expect = 6.8e-51
Identity = 113/276 (40.94%), Postives = 163/276 (59.06%), Query Frame = 0

Query: 45  SVPAFPVEIETQACRLDLSDELFGGVRQACNG-NLDRSRCCPVLAAWLFAAHARSALEVS 104
           ++PAFP + +   C LDL ++LF G++ AC+G  L + +CCPVL AWL++A++ +AL  S
Sbjct: 42  TIPAFPEQSDFSGCPLDLPEDLFHGIKSACSGKKLHKGKCCPVLGAWLYSAYSTTALSRS 101

Query: 105 GPAA------------SGETDLPLMPDDSQKCVESLQSSLVRRNIRIPQPNASCDAVLCF 164
             AA            + E D+PL+PDDS+ CV+ L  SL +R I + +PN +CD V C+
Sbjct: 102 ISAAARNSSSSSAAVTTPEEDMPLLPDDSETCVDGLGKSLRQRGIELTRPNETCDVVYCY 161

Query: 165 CGIRLHQISSLSCPAAFNLSGFQNASAAPTAAVRDLEKNCRNASYS---------GCTKC 224
           CGIRLH    LSC  AF ++            V  LE +C + S++         GC KC
Sbjct: 162 CGIRLH---PLSCSEAFRVN--DEGRLVGDERVDRLETDCLSGSHNNADGFSPLLGCNKC 221

Query: 225 LGALHKV---KGAPSRDSSTTERG--TKMFSRDCQLMGLTWLLARNKTAYIPTVSAVLRA 284
           L +L+K+   K + +R+ S  +R    KM ++DC LMGLTWLLA+N+TAY PTV++VLRA
Sbjct: 222 LNSLYKLNPKKTSGTRNPSKEDRNRTAKMHNKDCVLMGLTWLLAKNRTAYFPTVTSVLRA 281

Query: 285 IMYSAHPPRDSKCSPDQENMPLAVDSLQFEEAQSCS 294
           +M + H      C+   + MPLAVDS +F      S
Sbjct: 282 VMLN-HDGVPRSCALGSDGMPLAVDSSEFSNGSPTS 311