MS004846 (gene) Bitter gourd (TR) v1

BLAST of MS004846 vs. NCBI nr
Match: XP_022140618.1 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2-like [Momordica charantia])

HSP 1 Score: 759.6 bits (1960), Expect = 1.5e-215
Identity = 363/368 (98.64%), Postives = 364/368 (98.91%), Query Frame = 0

Query: 89  QPQKPHILCSPRDSVSLSIAESDSRHFHALIADHAKDATDVYQLCQDWPNFRVASDGTTQ 148
           QPQKPHILCSPRDSVSLSIAESDSRHFHALIADHAKDATDVYQLCQDWPNFRVASDGTTQ
Sbjct: 14  QPQKPHILCSPRDSVSLSIAESDSRHFHALIADHAKDATDVYQLCQDWPNFRVASDGTTQ 73

Query: 149 AIPLLTLRVTIFPYQGLSIGLSFLHVAADGPAFHHFVKSWASICKGQDFDGPTPVHDRTV 208
           AIPLLTLRVTIFPYQGLSIGLSFLHVA DGPAFHHFVKSWASICKGQDFDGPTPVHDRTV
Sbjct: 74  AIPLLTLRVTIFPYQGLSIGLSFLHVAVDGPAFHHFVKSWASICKGQDFDGPTPVHDRTV 133

Query: 209 VRDSNGLESTILQLQWSMAGPINFGSEPISKRLADMVRATFVLSKAQIEGLKSWARAESP 268
           VRD NGLESTILQLQWSMAGPINFGSEPISKRLADMVRATF LSKAQIEGLKSWARAESP
Sbjct: 134 VRDPNGLESTILQLQWSMAGPINFGSEPISKRLADMVRATFELSKAQIEGLKSWARAESP 193

Query: 269 HFSSFVAVCSLVWVCFIKSREGEGGNGKGSEVCCFAFAADCRKRLGYPMPAGYFGNCLAF 328
           HFSSFVAVCSLVWVCFIKSREGEGGNGKGSEVCCFAFAADCRKRLGYPMPAGYFGNCLAF
Sbjct: 194 HFSSFVAVCSLVWVCFIKSREGEGGNGKGSEVCCFAFAADCRKRLGYPMPAGYFGNCLAF 253

Query: 329 CHAVVEKGELLGGDGVIVGMKAIGKRVGEAEDEPLKETAEWASIWKKYKERGVVFAVAGS 388
           CHAVVEKGELLGGDGVIVGMKA GKRVGEAEDEPLKETAEWAS+WKKYKERGVVFAVAGS
Sbjct: 254 CHAVVEKGELLGGDGVIVGMKATGKRVGEAEDEPLKETAEWASVWKKYKERGVVFAVAGS 313

Query: 389 PRLGAYDVDFGWGRPIKTDVIHIDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWE 448
           PRLGAYDVDFGWGRPIKTDVIHIDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWE
Sbjct: 314 PRLGAYDVDFGWGRPIKTDVIHIDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWE 373

Query: 449 QTLNLFSC 457
           QTLNLFSC
Sbjct: 374 QTLNLFSC 381

BLAST of MS004846 vs. NCBI nr
Match: XP_023512859.1 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 595.5 bits (1534), Expect = 3.9e-166
Identity = 296/458 (64.63%), Postives = 344/458 (75.11%), Query Frame = 0

Query: 4   SVYTVKVLDECLVSPSPGSVPPTHIPLSFFDLMWLRTAPTRRVFFYKFPHPTTHFTNAIL 63
           S ++VKV+D+CLVSP PGSVP T +PL+FFDL+ LR APTRR+FFY+FPH T HFT+AIL
Sbjct: 5   SCFSVKVIDKCLVSPPPGSVPATLLPLTFFDLICLRYAPTRRLFFYEFPHSTAHFTHAIL 64

Query: 64  PSLKHSLSLALQQFFPFSANLIFPPQPQKPHILCSPRDSVSLSIAESDSRHFHALIADHA 123
           PSLK SLSL+LQ FFPF+ANLI PP P KPH L +P DSV L+IAESD  HFHALI DH 
Sbjct: 65  PSLKRSLSLSLQHFFPFAANLILPPHPHKPHFLYTPHDSVQLAIAESDPSHFHALIPDHP 124

Query: 124 KDATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHVAADGPAFHH 183
           KDA DVY L QDWP   + SDG TQ IPL+ L++TIFPY  LS+ +SFLHVAADGPA HH
Sbjct: 125 KDARDVYPLFQDWPMSCLLSDG-TQVIPLMALQITIFPYHALSLCVSFLHVAADGPALHH 184

Query: 184 FVKSWASICKGQDFDGPTPVHDRTVVRDSNGLESTILQLQWSMAGPINFGSEPISKRLAD 243
           F++SWASI  G   +GP P HDRTVV+D  GLES  L+     + PINF SEPIS+RL+D
Sbjct: 185 FIRSWASISAGNGLNGPLPSHDRTVVQDPEGLESAYLR---HHSEPINFDSEPISQRLSD 244

Query: 244 MVRATFVLSKAQIEGLKSWARAE---SPHFSSFVAVCSLVWVCFIKSRE-GEGGNGKGSE 303
            VRATF LSK QIE LK+WA ++   S  FSSFV   S+VWV FI SRE   GG+G   E
Sbjct: 245 KVRATFSLSKPQIERLKNWANSQITKSTQFSSFVVTSSVVWVSFIMSRELISGGSGAEDE 304

Query: 304 VCCFAFAADCR-KRLGYPMPAGYFGNCLAFCHAVVEKGELLGGDGVIVGMKAIGKRVGEA 363
            CCFAFAA+CR +RLGY +P  YFGNC AFC   VEK ELLG +G++V MKAI KRVGE 
Sbjct: 305 ACCFAFAANCRNERLGYRIPVEYFGNCFAFCLTAVEKSELLGENGLVVAMKAIEKRVGEV 364

Query: 364 EDEPLKETAEWASIWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPIKTDVIHIDSGSLFS 423
             E LK+     S WK++ ERG     AGSPRLGAYD +FGWGRPIKT+V+ +D G LFS
Sbjct: 365 NKETLKDVMAEMSAWKEFVERGAALCAAGSPRLGAYDANFGWGRPIKTEVVQVDRGRLFS 424

Query: 424 LGESRNENGGIEVGLALSTAHMARFVAVWEQTLNLFSC 457
           +GESRNENGGIEVGL L +AHMA FVAVWEQ L L  C
Sbjct: 425 IGESRNENGGIEVGLGLGSAHMASFVAVWEQNLKLLGC 458

BLAST of MS004846 vs. NCBI nr
Match: KAE8075836.1 (hypothetical protein FH972_014523 [Carpinus fangiana])

HSP 1 Score: 449.5 bits (1155), Expect = 3.4e-122
Identity = 227/467 (48.61%), Postives = 302/467 (64.67%), Query Frame = 0

Query: 1   MAESVYTVKVLDECLVSPSPGSVPPTHIPLSFFDLMWLRTAPTRRVFFYKFPHPTTHFTN 60
           MA+  Y V V+++  V+P P SVP T + LSFFD+ WL   P +R+FFY+ P PT +FT 
Sbjct: 1   MAQPTYRVSVIEQSQVAPPPSSVPATTLSLSFFDIPWLLCRPMQRLFFYELPQPTNYFTQ 60

Query: 61  AILPSLKHSLSLALQQFFPFSANLIFPPQPQKPHILCSPRDSVSLSIAESDSRHFHALIA 120
           AILP+LKHSLSL LQ+FFP +ANL+ PPQ  KPHI  +  +SV  ++AE+ +  F+ L+A
Sbjct: 61  AILPTLKHSLSLTLQRFFPLAANLVCPPQTHKPHIFYAEGNSVPFTVAEA-AGDFNRLVA 120

Query: 121 DHAKDATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHVAADGPA 180
           ++ +D  +++      P   V+SD  T+ IPL+ ++VT+FP +G+ IG+ F HVAADG A
Sbjct: 121 NYPRDVRELHHFVPQLPPAHVSSD-KTRVIPLMAMQVTVFPNEGICIGIIFCHVAADGRA 180

Query: 181 FHHFVKSWASICK-GQD---FDGPTPVHDRTVVRDSNGLESTILQLQWSMAGPINFGSEP 240
           FHHF+KSWAS+C+ G D    D   P +DR V++D NGLE  +L+  WS  G        
Sbjct: 181 FHHFMKSWASVCRTGGDLTCLDRSPPFNDRAVMKDPNGLEQNLLEDWWSW-GSAWTDDTG 240

Query: 241 ISKRLADMVRATFVLSKAQIEGLKSWAR--------AESPHFSSFVAVCSLVWVCFIKSR 300
           +    A+ VRATFVLS+A IEGLK W +        +E  H S+FVA C+ +WVC IKS+
Sbjct: 241 LINDFAEKVRATFVLSRAHIEGLKQWVKGQCMNKEDSEPLHVSTFVATCAFIWVCLIKSQ 300

Query: 301 -EGEGGNGKGSEVCCFAFAADCRKRLGYPMPAGYFGNCLAFCHAVVEKGELLGGDGVIVG 360
             G       SE+  FAF ADCR RL +P+P  YFGNCLA C   +EKGELLG  G+   
Sbjct: 301 YNGVSDFSDNSELFYFAFVADCRNRLKFPVPCTYFGNCLAICIVALEKGELLGETGIFEA 360

Query: 361 MKAIGKRVGEAEDEPLKETAEWASIWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPIKTD 420
           +K IG +V ++E E L+    W   WK+  E G +  VAGSPRLG Y+ DFGWGRP K++
Sbjct: 361 VKVIGNKVRQSETEALRGAETWLLDWKEKSETGTLVTVAGSPRLGVYETDFGWGRPKKSE 420

Query: 421 VIHIDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTLNLF 455
           V+HID     SL E R+E GGIEVGLAL   ++A F A WEQ+L LF
Sbjct: 421 VVHIDVTGAISLAECRDEEGGIEVGLALRRKNIADFTAAWEQSLKLF 464

BLAST of MS004846 vs. NCBI nr
Match: XP_022140373.1 (LOW QUALITY PROTEIN: coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Momordica charantia])

HSP 1 Score: 447.2 bits (1149), Expect = 1.7e-121
Identity = 230/469 (49.04%), Postives = 293/469 (62.47%), Query Frame = 0

Query: 6   YTVKVLDECLVSPSPGSVPPTHIPLSFFDLMWLRTAPTRRVFFYKFPHPTTHFTNAILPS 65
           YT KVL+ C +SP  GSVP TH+PLSF D++W+   P RR+FFY FPH T HF +AI P+
Sbjct: 8   YTAKVLNHCRISPPAGSVPTTHLPLSFLDMLWIVCRPMRRLFFYHFPHSTHHFIHAIFPT 67

Query: 66  LKHSLSLALQQFFPFSANLIFPPQPQKPHILCSPRDSVSLSIAESDSRHFHALIADHAKD 125
           LK SLSLALQ FFP + N I PP P +PHILC+P DSV L++AES    F  LIADH + 
Sbjct: 68  LKRSLSLALQHFFPLAGNFILPPPPHRPHILCTPHDSVPLTVAESSGCDFDRLIADHPRP 127

Query: 126 ATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHVAADGPAFHHFV 185
           A   Y L  D PN R + DG  + +P+  L++T+FP QGL I ++F HV ADG A H F+
Sbjct: 128 AKHFYPLVPDLPNSRASPDG-ARVVPVFALQITVFPNQGLCISIAFHHVVADGMAAHDFL 187

Query: 186 KSWASICK----GQDFDGPTPVHDRTVVRDSNGLESTILQ--LQW-SMAGPINFGSEPIS 245
           KS ASI +        DGP P HDR  V+D NGLES  LQ    W S+  P+      I 
Sbjct: 188 KSXASIHRNGGGSGSLDGPLPYHDRAAVKDPNGLESIFLQELSYWDSLWNPVP-DKPIID 247

Query: 246 KRLADMVRATFVLSKAQIEGLKSWARAESPH----------FSSFVAVCSLVWVCFIKSR 305
              AD VRATFV++K QIE LK+W   ++ H           S+ +  CSL+W C IKS 
Sbjct: 248 LAPADKVRATFVINKTQIERLKTWISNQAAHDDNEELKPLRLSALIVTCSLIWGCLIKST 307

Query: 306 EGEGGNG----KGSEVCCFAFAADCRKRLGYPMPAGYFGNCLAFCHAVVEKGELLGGDGV 365
           EGE        +  E+C F   ADCR RL  P+P  YFGNCL     V++K ELL  +  
Sbjct: 308 EGEFAQNDLPDELDELCFFVLLADCRNRLKDPLPPTYFGNCLDMGAVVLKKSELLKSNSS 367

Query: 366 IVGMKAIGKRVGEAEDEPLKETAEWASIWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPI 425
           +V  KAIGKRV E E EPLK   +W S W+  + + ++  +AGSP+L  YD DFGWG+P 
Sbjct: 368 VVAAKAIGKRVEEFESEPLKTADKWFSEWRCLESKHLL-TIAGSPKLRVYDTDFGWGKPK 427

Query: 426 KTDVIHIDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTLNL 454
           K++V+H+DS    SL E R+E GGIEVGL L+  HM RF+A+WE+ LN+
Sbjct: 428 KSEVLHVDSSLAISLQECRDEKGGIEVGLGLTKTHMDRFIAIWEENLNV 473

BLAST of MS004846 vs. NCBI nr
Match: XP_023533558.1 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 438.0 bits (1125), Expect = 1.0e-118
Identity = 221/467 (47.32%), Postives = 295/467 (63.17%), Query Frame = 0

Query: 1   MAESVYTVKVLDECLVSPSPGSVPPTHIPLSFFDLMWLRTAPTRRVFFYKFPHPTTHFTN 60
           MA+  YT K+L+   +SP  GSVP T +PLSF D +W+   P RR+FF++FPH T HF +
Sbjct: 39  MADVDYTAKLLNLYRISPPAGSVPTTLLPLSFLDFIWMICRPMRRLFFFQFPHSTNHFMD 98

Query: 61  AILPSLKHSLSLALQQFFPFSANLIFPPQPQKPHILCSPRDSVSLSIAESDSRHFHALIA 120
           ++ P+LK SLSL+LQ FFP + NLI PPQP KPHIL +P DSV L++AES    F  LIA
Sbjct: 99  SVFPTLKRSLSLSLQHFFPLAGNLILPPQPHKPHILYTPHDSVPLTVAESTGGDFTTLIA 158

Query: 121 DHAKDATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHVAADGPA 180
           D+ +DA   Y    D P  R+  DG ++AIP   +++TIFP QGL I ++F HV ADG A
Sbjct: 159 DYPRDAKHFYPFVPDMPESRLLPDG-SRAIPNFAIQITIFPNQGLCICIAFQHVVADGIA 218

Query: 181 FHHFVKSWASICK----GQDFDGPTPVHDRTVVRDSNGLESTILQ--LQW-SMAGPINFG 240
            HHF+KSWASI +       F+GP P H+R  ++D  GLES  LQ    W S+  P+ + 
Sbjct: 219 IHHFIKSWASISRNGGTSDSFEGPLPCHNRAAIKDVKGLESIFLQELSSWDSLWNPVPY- 278

Query: 241 SEPISKRLADMVRATFVLSKAQIEGLKSWARAESPH--------FSSFVAVCSLVWVCFI 300
              I +   D VR T VL++  IE LK+W  AES +         SSF+  CSLVW C I
Sbjct: 279 KPIIDQAPVDRVRTTLVLTRPMIEKLKTWVSAESNNDEELKPLRLSSFIVTCSLVWGCLI 338

Query: 301 KSREGEGGNGKG-SEVCCFAFAADCRKRLGYPMPAGYFGNCLAFCHAVVEKGELLGGDGV 360
           KS + E  + +   E+C F F ADCR R+  P+P  YFGNCL      ++K EL+  + +
Sbjct: 339 KSTQSEASDSEDEDELCYFGFLADCRNRVKDPLPPTYFGNCLDVGVVELKKSELVKRNSI 398

Query: 361 IVGMKAIGKRVGEAEDEPLKETAEWASIWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPI 420
           ++  KAIG RV E E+EPLK   +W S+WK Y +      +AGSP+L AYD DFGWGR +
Sbjct: 399 VMAAKAIGNRVEEFENEPLKVAEKWFSLWKTYSKDKHFVTIAGSPKLKAYDTDFGWGRAM 458

Query: 421 KTDVIHIDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTL 452
           K++V+H+D+    SL +SR+E GGIE+GL L    M RF+A+WEQ L
Sbjct: 459 KSEVLHVDNSESISLQDSRDEQGGIEIGLGLHNTQMDRFMAIWEQNL 503

BLAST of MS004846 vs. ExPASy Swiss-Prot
Match: Q9ZWB4 (Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=3AT1 PE=1 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 1.1e-75
Identity = 164/465 (35.27%), Postives = 251/465 (53.98%), Query Frame = 0

Query: 9   KVLDECLVSPSPGSVPPTHIPLSFFDLMWLRTAPTRRVFFYKFPHPTTHFTNAILPSLKH 68
           K+++ C +SP  G+VP T +PL+FFD  WL       +FF+ + + T  F    +P+LKH
Sbjct: 9   KIIETCHISPPKGTVPSTTLPLTFFDAPWLSLPLADSLFFFSYQNSTESFLQDFVPNLKH 68

Query: 69  SLSLALQQFFPFSANLIFPPQPQKPHILCSP-RDSVSLSIAESDSRHFHALIADHAKDAT 128
           SLS+ LQ FFP++  LI PP+P  P++  +  +DS+  ++AES    F  L +D  KD +
Sbjct: 69  SLSITLQHFFPYAGKLIIPPRPDPPYLHYNDGQDSLVFTVAESTETDFDQLKSDSPKDIS 128

Query: 129 DVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHVAADGPAFHHFVKS 188
            ++ +    P   V+ +G  Q  P++ ++VTIFP  G+ IG S  HV ADG  F HF+K 
Sbjct: 129 VLHGVLPKLPPPHVSPEG-IQMRPIMAMQVTIFPGAGICIGNSATHVVADGVTFSHFMKY 188

Query: 189 WASICKGQDFDGPT------PVHD-RTVVRDSNGLESTILQLQWSMAGPINFGSEPISKR 248
           W S+ K    D  T      P+H  R +++D   + +  L+  WS     N         
Sbjct: 189 WMSLTKSSGKDPATVLLPSLPIHSCRNMIKDPGEVGAGHLERFWSQ----NSAKHSSHVT 248

Query: 249 LADMVRATFVLSKAQIEGLKSWARAESPH---FSSFVAVCSLVWVCFIKS----REGEGG 308
             +MVRATF LS+ QI+ LKSW   +S +    S+FV   + +WV  IK+     E +  
Sbjct: 249 PENMVRATFTLSRKQIDNLKSWVTEQSENQSPVSTFVVTLAFIWVSLIKTLVQDSETKAN 308

Query: 309 NGKGSEVCCFAFAADCRKRLGY--PMPAGYFGNCLAFCHAVVEKGELLGGDGVIVGMKAI 368
                EV       DCR RL Y  P+P  YFGNC+A     V+K +LLG   V+    AI
Sbjct: 309 EEDKDEVFHLMINVDCRNRLKYTQPIPQTYFGNCMAPGIVSVKKHDLLGEKCVLAASDAI 368

Query: 369 GKRVGEAEDEPLKETA-EWASIWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPIKTDVIH 428
             R+ +     L +TA  W    +K+       ++AG+P+LG YD+DFG G+P K +++H
Sbjct: 369 TARIKDMLSSDLLKTAPRWGQGVRKWVMSHYPTSIAGAPKLGLYDMDFGLGKPCKMEIVH 428

Query: 429 IDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTLNLFS 456
           I++G   +  ESR+ + G+E+G+AL    M  F ++ +Q +  F+
Sbjct: 429 IETGGSIAFSESRDGSNGVEIGIALEKKKMDVFDSILQQGIKKFA 468

BLAST of MS004846 vs. ExPASy Swiss-Prot
Match: Q9LR73 (Coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=3AT2 PE=1 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 7.3e-75
Identity = 162/469 (34.54%), Postives = 250/469 (53.30%), Query Frame = 0

Query: 1   MAESVYTVKVLDECLVSPSPGSVPPTHIPLSFFDLMWLRTAPTRRVFFYKFPHPTTHFTN 60
           MA  +    +++ C +SP  G+V  T +PL+FFD  WL       +FF+ + + T  F  
Sbjct: 1   MAAQLQPYNIIETCHISPPKGTVASTTLPLTFFDAPWLSLPLADSLFFFSYQNSTESFLQ 60

Query: 61  AILPSLKHSLSLALQQFFPFSANLIFPPQPQKPHI-LCSPRDSVSLSIAESDSRHFHALI 120
             +P+LKHSLS+ LQ FFP++  LI PP+P  P++   +  DS+  ++AES    F  L 
Sbjct: 61  DFVPNLKHSLSITLQHFFPYAGKLIIPPRPDPPYLHYNAGEDSLVFTVAESTETDFDQLK 120

Query: 121 ADHAKDATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHVAADGP 180
           +D  KD + ++ +    P   V+ +G  Q  P++ ++VTIFP  G+ IG S  HV ADG 
Sbjct: 121 SDSPKDISVLHGVLPKLPPPHVSPEG-IQMRPIMAMQVTIFPGAGICIGNSATHVVADGV 180

Query: 181 AFHHFVKSWASICKGQDFDGPT------PVHD-RTVVRDSNGLESTILQLQWSMAGPINF 240
            F HF+K W S+ K    D  T      P+H  R +++D   + +  L+  WS     N 
Sbjct: 181 TFSHFMKYWMSLTKSSGKDPATVLLPSLPIHSCRNIIKDPGEVAAGHLERFWSQ----NS 240

Query: 241 GSEPISKRLADMVRATFVLSKAQIEGLKSWARAESPH---FSSFVAVCSLVWVCFIKSRE 300
                     +MVRATF LS+ QI+ LKSW   +S +    S+FV   + +WV  IK+  
Sbjct: 241 AKHSSHVTPENMVRATFTLSRKQIDNLKSWVTEQSENQSPVSTFVVTLAFIWVSLIKTLV 300

Query: 301 GEGGNGKGSEVCCFAFAADCRKRLGY--PMPAGYFGNCLAFCHAVVEKGELLGGDGVIVG 360
            +       EV       DCR RL Y  P+P  YFGNC+A     V+K +LLG   V+  
Sbjct: 301 QDSETEAKDEVFHLMINVDCRNRLKYTQPIPQTYFGNCMAPGIVSVKKHDLLGEKCVMAA 360

Query: 361 MKAIGKRVGEAEDEPLKETA-EWASIWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPIKT 420
             AI  R+ +     L +TA  W    +K+       ++AG+P+LG YD+DFG G+P K 
Sbjct: 361 SDAITARIKDMLSSDLLKTAPRWGQGVRKWVMSHYPTSIAGAPKLGLYDMDFGLGKPCKM 420

Query: 421 DVIHIDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTLNLFS 456
           +++HI++G   +  ESR+ + G+E+G+AL    M  F ++ ++ +  F+
Sbjct: 421 EIVHIETGGSIAFSESRDGSNGVEIGIALEKKKMDVFDSLLQKGIKKFA 464

BLAST of MS004846 vs. ExPASy Swiss-Prot
Match: Q8GSN8 (Malonyl-coenzyme A:anthocyanin 3-O-glucoside-6''-O-malonyltransferase OS=Dahlia pinnata OX=101596 GN=3MAT PE=1 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 7.8e-69
Identity = 157/465 (33.76%), Postives = 240/465 (51.61%), Query Frame = 0

Query: 3   ESVYTVKVLDECLVSPSPGSVPPTHIPLSFFDLMWLRTAPTRRVFFYKFPHPTTHFTNAI 62
           +++  + +L+   +SP P ++    +PL+FFD+ WL   P   ++FY FP+  +HFT  +
Sbjct: 2   DNIPNLTILEHSRISPPPSTIGHRSLPLTFFDIAWLLFPPVHHLYFYHFPYSKSHFTETV 61

Query: 63  LPSLKHSLSLALQQFFPFSANLIFPPQP----QKPHILCSPRDSVSLSIAESDSRHFHAL 122
           +P+LKHSLS+ LQ +FPF   LI  P P    +KP I     DSV+L+ AE+ +  F+ L
Sbjct: 62  IPNLKHSLSITLQHYFPFVGKLIVYPNPHDSTRKPEIRHVEGDSVALTFAET-TLDFNDL 121

Query: 123 IADHAKDATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHVAADG 182
            A+H +   + Y L     N    SD  T  +P+ +++VT FP  G+SIGL+  H  +D 
Sbjct: 122 SANHPRKCENFYPLVPPLGNAVKESDYVT--LPVFSVQVTYFPNSGISIGLTNHHSLSDA 181

Query: 183 PAFHHFVKSWASICK-GQD----FDGPTPVHDRTVVRD---SNGLESTILQLQWSMAGPI 242
                F+K+WAS+C+ G+D     +G  PV DR VV      N L  T  +L      P 
Sbjct: 182 NTRFGFLKAWASVCETGEDQPFLKNGSPPVFDRVVVNPQLYENRLNQT--RLGTFYQAPS 241

Query: 243 NFGSEPISKRLADMVRATFVLSKAQIEGLKSWARAESP---HFSSFVAVCSLVWVCFIKS 302
             GS       +D VRATFVL++  I GLK     + P   + SSF   C  +W C +KS
Sbjct: 242 LVGSS------SDRVRATFVLARTHISGLKKQVLTQLPMLEYTSSFTVTCGYIWSCIVKS 301

Query: 303 REGEGGNGKGSEVCCFAFAADCRKRLGYPMPAGYFGNCLAFCHAVVEKGELLGGDGVIVG 362
               G      E+  F  +  CR RL  P+P  YFGNC A C   ++ G L G +G ++ 
Sbjct: 302 LVNMGEKKGEDELEQFIVSVGCRSRLDPPLPENYFGNCSAPCIVTIKNGVLKGENGFVMA 361

Query: 363 MKAIGKRVGEAEDEPLKETAEWASIW-KKYKERGVVFAVAGSPRLGAYDVDFGWGRPIKT 422
            K IG+ + +  ++      E+A  W   +K       ++G+P+L  YD+DFGWG+ +K 
Sbjct: 362 AKLIGEGISKMVNKK-GGILEYADRWYDGFKIPARKMGISGTPKLNFYDIDFGWGKAMKY 421

Query: 423 DVIHIDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTL 452
           +V+ ID  +  SL   +      E+G+   +  M  F  ++   L
Sbjct: 422 EVVSIDYSASVSLSACKESAQDFEIGVCFPSMQMEAFGKIFNDGL 454

BLAST of MS004846 vs. ExPASy Swiss-Prot
Match: Q9ZWR8 (Anthocyanin 5-aromatic acyltransferase OS=Gentiana triflora OX=55190 PE=1 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 1.7e-68
Identity = 165/473 (34.88%), Postives = 254/473 (53.70%), Query Frame = 0

Query: 3   ESVYTVKVLDECLVS-PSPGSVPPTHIPLSFFDLMWLRTAPTRRVFFYKFPHPTTHFTNA 62
           E +  VKVL++C V+ PS  +     +P++FFD+ WL     + + FY FP+P THF + 
Sbjct: 2   EQIQMVKVLEKCQVTPPSDTTDVELSLPVTFFDIPWLHLNKMQSLLFYDFPYPRTHFLDT 61

Query: 63  ILPSLKHSLSLALQQFFPFSANLIFP-PQPQKPHILCS--PRDSVSLSIAESDSRHFHAL 122
           ++P+LK SLSL L+ + P S NL+ P    + P    S    DS++L +AESD + F  L
Sbjct: 62  VIPNLKASLSLTLKHYVPLSGNLLMPIKSGEMPKFQYSRDEGDSITLIVAESD-QDFDYL 121

Query: 123 IADHAKDATDVYQLCQDWPN-FRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHVAAD 182
                 D+ D++ L    P   R   D   + IPL+ ++VT+FP +G+++ L+  H  AD
Sbjct: 122 KGHQLVDSNDLHGLFYVMPRVIRTMQD--YKVIPLVAVQVTVFPNRGIAVALTAHHSIAD 181

Query: 183 GPAFHHFVKSWASICK-GQDFD----GPTPVHDRTVVRDSNGLESTIL-QLQWSMAGPIN 242
             +F  F+ +WA I K G+D D       P  DR++++D  GLE T   ++Q  +     
Sbjct: 182 AKSFVMFINAWAYINKFGKDADLLSANLLPSFDRSIIKDLYGLEETFWNEMQDVLEMFSR 241

Query: 243 FGSEPISKRLADMVRATFVLSKAQIEGLKSWA---RAESP--HFSSFVAVCSLVWVCFIK 302
           FGS+P      + VRAT+VLS A+I+ LK+     R   P    ++F   C  VW C +K
Sbjct: 242 FGSKP---PRFNKVRATYVLSLAEIQKLKNKVLNLRGSEPTIRVTTFTMTCGYVWTCMVK 301

Query: 303 SRE---GEGGNGKGSEVCCFAFAADCRKRLGYPMPAGYFGNCLAFCHAVVEKGELLGGDG 362
           S++    E  +   +E+  F+F ADCR  L  P P  YFGNCLA C A     EL+G  G
Sbjct: 302 SKDDVVSEESSNDENELEYFSFTADCRGLLTPPCPPNYFGNCLASCVAKATHKELVGDKG 361

Query: 363 VIVGMKAIGKRVGEA---EDEPLKETAEWASIWKKYKERGV----VFAVAGSPRLGAYDV 422
           ++V + AIG+ + +    E   L +   W S     +  G+       + GSP+  +Y V
Sbjct: 362 LLVAVAAIGEAIEKRLHNEKGVLADAKTWLS-----ESNGIPSKRFLGITGSPKFDSYGV 421

Query: 423 DFGWGRPIKTDVIHIDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQ 450
           DFGWG+P K D+  +D   L  + +SR+   G+E+G++L   HM  F  ++E+
Sbjct: 422 DFGWGKPAKFDITSVDYAELIYVIQSRDFEKGVEIGVSLPKIHMDAFAKIFEE 463

BLAST of MS004846 vs. ExPASy Swiss-Prot
Match: Q9LRQ7 (BAHD acyltransferase At3g29680 OS=Arabidopsis thaliana OX=3702 GN=At3g29680 PE=2 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 3.6e-66
Identity = 170/456 (37.28%), Postives = 245/456 (53.73%), Query Frame = 0

Query: 16  VSPSPGSVPPTHIPLSFFDLMWLRTAPTRRVFFYKFPHPT-THFTNAILPSLKHSLSLAL 75
           VS    SV P  +PL+FFDL+WL+  P  RV FYK    +   F ++ILP L+ SLSL L
Sbjct: 11  VSLVTNSVEPLVLPLTFFDLLWLKLNPIERVTFYKLTESSRDSFFSSILPKLEQSLSLVL 70

Query: 76  QQFFPFSANLIFPPQPQKPHILCSPRDSVSLSIAESDSRHFHALIADHAKDATDVYQLCQ 135
             F P S +L + PQ  KPHI+  P+D+VSL++ ES++  F  + +   +  T++  L  
Sbjct: 71  SHFLPLSGHLKWNPQDPKPHIVIFPKDTVSLTVVESEA-DFSYISSKELRLETELRPLV- 130

Query: 136 DWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHVAADGPAFHHFVKSWASICK- 195
             P  +V+SD  +    LL+L++T+FP QG SIG +  HV  DG     F KSWA ICK 
Sbjct: 131 --PELQVSSDSAS----LLSLQITLFPNQGFSIGTTVHHVVMDGKTASKFHKSWAHICKH 190

Query: 196 ---GQDFDGPTPVHDRTVVRDSNGLESTILQLQWSMAGPINFG---SEPISKRL-ADMVR 255
               QDFD PT V DRTV+    GLE  I QL   ++   ++    + P +K +  D+VR
Sbjct: 191 GTTPQDFDLPT-VLDRTVINVPAGLEQKIFQLSSYISEEKDYARTLTLPPAKEIDNDVVR 250

Query: 256 ATFVLSKAQIEGLKSWARAESP----HFSSFVAVCSLVWVCFIKSREGEGGNGKGSEVCC 315
            T  L++  IE LK  A+ ES     H S+FV   + V  C +KS  G+      +    
Sbjct: 251 VTLELTEVDIEKLKERAKNESTRSDLHLSTFVVSYAYVLTCMVKSCGGD-----ANRPVR 310

Query: 316 FAFAADCRKRLGYPMPAGYFGNC-LAFCHAVVEKGELLGGDGVIVGMKAIGKRV---GEA 375
           F +AAD R RL  P+P  YFGNC L       +    LG DG + G++ +   V   G  
Sbjct: 311 FMYAADFRNRLDPPVPLTYFGNCVLPIDFNGYKATTFLGKDGYVNGVEILSDSVRGLGSR 370

Query: 376 EDEPLKETAEWASIWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPIKTDVIHIDSGSLFS 435
             E + E  E  +   K   + V   V GS + G Y  DFGWGRP+KTDV+ +   + FS
Sbjct: 371 NIESIWEVYEDGTKNMKLDTQNV--TVTGSNQFGIYGSDFGWGRPVKTDVMSLYKNNEFS 430

Query: 436 LGESRNENGGIEVGLALSTAHMARFVAVWEQTLNLF 455
           +   R+E GG+E+G++L    M  F++++    +++
Sbjct: 431 MSARRDEIGGLEIGISLKKCEMNVFLSLFTSDFDIY 450

BLAST of MS004846 vs. ExPASy TrEMBL
Match: A0A6J1CIE1 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 2-like OS=Momordica charantia OX=3673 GN=LOC111011225 PE=3 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 7.5e-216
Identity = 363/368 (98.64%), Postives = 364/368 (98.91%), Query Frame = 0

Query: 89  QPQKPHILCSPRDSVSLSIAESDSRHFHALIADHAKDATDVYQLCQDWPNFRVASDGTTQ 148
           QPQKPHILCSPRDSVSLSIAESDSRHFHALIADHAKDATDVYQLCQDWPNFRVASDGTTQ
Sbjct: 14  QPQKPHILCSPRDSVSLSIAESDSRHFHALIADHAKDATDVYQLCQDWPNFRVASDGTTQ 73

Query: 149 AIPLLTLRVTIFPYQGLSIGLSFLHVAADGPAFHHFVKSWASICKGQDFDGPTPVHDRTV 208
           AIPLLTLRVTIFPYQGLSIGLSFLHVA DGPAFHHFVKSWASICKGQDFDGPTPVHDRTV
Sbjct: 74  AIPLLTLRVTIFPYQGLSIGLSFLHVAVDGPAFHHFVKSWASICKGQDFDGPTPVHDRTV 133

Query: 209 VRDSNGLESTILQLQWSMAGPINFGSEPISKRLADMVRATFVLSKAQIEGLKSWARAESP 268
           VRD NGLESTILQLQWSMAGPINFGSEPISKRLADMVRATF LSKAQIEGLKSWARAESP
Sbjct: 134 VRDPNGLESTILQLQWSMAGPINFGSEPISKRLADMVRATFELSKAQIEGLKSWARAESP 193

Query: 269 HFSSFVAVCSLVWVCFIKSREGEGGNGKGSEVCCFAFAADCRKRLGYPMPAGYFGNCLAF 328
           HFSSFVAVCSLVWVCFIKSREGEGGNGKGSEVCCFAFAADCRKRLGYPMPAGYFGNCLAF
Sbjct: 194 HFSSFVAVCSLVWVCFIKSREGEGGNGKGSEVCCFAFAADCRKRLGYPMPAGYFGNCLAF 253

Query: 329 CHAVVEKGELLGGDGVIVGMKAIGKRVGEAEDEPLKETAEWASIWKKYKERGVVFAVAGS 388
           CHAVVEKGELLGGDGVIVGMKA GKRVGEAEDEPLKETAEWAS+WKKYKERGVVFAVAGS
Sbjct: 254 CHAVVEKGELLGGDGVIVGMKATGKRVGEAEDEPLKETAEWASVWKKYKERGVVFAVAGS 313

Query: 389 PRLGAYDVDFGWGRPIKTDVIHIDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWE 448
           PRLGAYDVDFGWGRPIKTDVIHIDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWE
Sbjct: 314 PRLGAYDVDFGWGRPIKTDVIHIDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWE 373

Query: 449 QTLNLFSC 457
           QTLNLFSC
Sbjct: 374 QTLNLFSC 381

BLAST of MS004846 vs. ExPASy TrEMBL
Match: A0A5N6RBV5 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_014523 PE=3 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 1.7e-122
Identity = 227/467 (48.61%), Postives = 302/467 (64.67%), Query Frame = 0

Query: 1   MAESVYTVKVLDECLVSPSPGSVPPTHIPLSFFDLMWLRTAPTRRVFFYKFPHPTTHFTN 60
           MA+  Y V V+++  V+P P SVP T + LSFFD+ WL   P +R+FFY+ P PT +FT 
Sbjct: 1   MAQPTYRVSVIEQSQVAPPPSSVPATTLSLSFFDIPWLLCRPMQRLFFYELPQPTNYFTQ 60

Query: 61  AILPSLKHSLSLALQQFFPFSANLIFPPQPQKPHILCSPRDSVSLSIAESDSRHFHALIA 120
           AILP+LKHSLSL LQ+FFP +ANL+ PPQ  KPHI  +  +SV  ++AE+ +  F+ L+A
Sbjct: 61  AILPTLKHSLSLTLQRFFPLAANLVCPPQTHKPHIFYAEGNSVPFTVAEA-AGDFNRLVA 120

Query: 121 DHAKDATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHVAADGPA 180
           ++ +D  +++      P   V+SD  T+ IPL+ ++VT+FP +G+ IG+ F HVAADG A
Sbjct: 121 NYPRDVRELHHFVPQLPPAHVSSD-KTRVIPLMAMQVTVFPNEGICIGIIFCHVAADGRA 180

Query: 181 FHHFVKSWASICK-GQD---FDGPTPVHDRTVVRDSNGLESTILQLQWSMAGPINFGSEP 240
           FHHF+KSWAS+C+ G D    D   P +DR V++D NGLE  +L+  WS  G        
Sbjct: 181 FHHFMKSWASVCRTGGDLTCLDRSPPFNDRAVMKDPNGLEQNLLEDWWSW-GSAWTDDTG 240

Query: 241 ISKRLADMVRATFVLSKAQIEGLKSWAR--------AESPHFSSFVAVCSLVWVCFIKSR 300
           +    A+ VRATFVLS+A IEGLK W +        +E  H S+FVA C+ +WVC IKS+
Sbjct: 241 LINDFAEKVRATFVLSRAHIEGLKQWVKGQCMNKEDSEPLHVSTFVATCAFIWVCLIKSQ 300

Query: 301 -EGEGGNGKGSEVCCFAFAADCRKRLGYPMPAGYFGNCLAFCHAVVEKGELLGGDGVIVG 360
             G       SE+  FAF ADCR RL +P+P  YFGNCLA C   +EKGELLG  G+   
Sbjct: 301 YNGVSDFSDNSELFYFAFVADCRNRLKFPVPCTYFGNCLAICIVALEKGELLGETGIFEA 360

Query: 361 MKAIGKRVGEAEDEPLKETAEWASIWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPIKTD 420
           +K IG +V ++E E L+    W   WK+  E G +  VAGSPRLG Y+ DFGWGRP K++
Sbjct: 361 VKVIGNKVRQSETEALRGAETWLLDWKEKSETGTLVTVAGSPRLGVYETDFGWGRPKKSE 420

Query: 421 VIHIDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTLNLF 455
           V+HID     SL E R+E GGIEVGLAL   ++A F A WEQ+L LF
Sbjct: 421 VVHIDVTGAISLAECRDEEGGIEVGLALRRKNIADFTAAWEQSLKLF 464

BLAST of MS004846 vs. ExPASy TrEMBL
Match: A0A6J1CHV4 (LOW QUALITY PROTEIN: coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=Momordica charantia OX=3673 GN=LOC111011063 PE=3 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 8.2e-122
Identity = 230/469 (49.04%), Postives = 293/469 (62.47%), Query Frame = 0

Query: 6   YTVKVLDECLVSPSPGSVPPTHIPLSFFDLMWLRTAPTRRVFFYKFPHPTTHFTNAILPS 65
           YT KVL+ C +SP  GSVP TH+PLSF D++W+   P RR+FFY FPH T HF +AI P+
Sbjct: 8   YTAKVLNHCRISPPAGSVPTTHLPLSFLDMLWIVCRPMRRLFFYHFPHSTHHFIHAIFPT 67

Query: 66  LKHSLSLALQQFFPFSANLIFPPQPQKPHILCSPRDSVSLSIAESDSRHFHALIADHAKD 125
           LK SLSLALQ FFP + N I PP P +PHILC+P DSV L++AES    F  LIADH + 
Sbjct: 68  LKRSLSLALQHFFPLAGNFILPPPPHRPHILCTPHDSVPLTVAESSGCDFDRLIADHPRP 127

Query: 126 ATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHVAADGPAFHHFV 185
           A   Y L  D PN R + DG  + +P+  L++T+FP QGL I ++F HV ADG A H F+
Sbjct: 128 AKHFYPLVPDLPNSRASPDG-ARVVPVFALQITVFPNQGLCISIAFHHVVADGMAAHDFL 187

Query: 186 KSWASICK----GQDFDGPTPVHDRTVVRDSNGLESTILQ--LQW-SMAGPINFGSEPIS 245
           KS ASI +        DGP P HDR  V+D NGLES  LQ    W S+  P+      I 
Sbjct: 188 KSXASIHRNGGGSGSLDGPLPYHDRAAVKDPNGLESIFLQELSYWDSLWNPVP-DKPIID 247

Query: 246 KRLADMVRATFVLSKAQIEGLKSWARAESPH----------FSSFVAVCSLVWVCFIKSR 305
              AD VRATFV++K QIE LK+W   ++ H           S+ +  CSL+W C IKS 
Sbjct: 248 LAPADKVRATFVINKTQIERLKTWISNQAAHDDNEELKPLRLSALIVTCSLIWGCLIKST 307

Query: 306 EGEGGNG----KGSEVCCFAFAADCRKRLGYPMPAGYFGNCLAFCHAVVEKGELLGGDGV 365
           EGE        +  E+C F   ADCR RL  P+P  YFGNCL     V++K ELL  +  
Sbjct: 308 EGEFAQNDLPDELDELCFFVLLADCRNRLKDPLPPTYFGNCLDMGAVVLKKSELLKSNSS 367

Query: 366 IVGMKAIGKRVGEAEDEPLKETAEWASIWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPI 425
           +V  KAIGKRV E E EPLK   +W S W+  + + ++  +AGSP+L  YD DFGWG+P 
Sbjct: 368 VVAAKAIGKRVEEFESEPLKTADKWFSEWRCLESKHLL-TIAGSPKLRVYDTDFGWGKPK 427

Query: 426 KTDVIHIDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTLNL 454
           K++V+H+DS    SL E R+E GGIEVGL L+  HM RF+A+WE+ LN+
Sbjct: 428 KSEVLHVDSSLAISLQECRDEKGGIEVGLGLTKTHMDRFIAIWEENLNV 473

BLAST of MS004846 vs. ExPASy TrEMBL
Match: A0A6J1I4H8 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111469803 PE=3 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 6.5e-119
Identity = 222/467 (47.54%), Postives = 295/467 (63.17%), Query Frame = 0

Query: 1   MAESVYTVKVLDECLVSPSPGSVPPTHIPLSFFDLMWLRTAPTRRVFFYKFPHPTTHFTN 60
           MA+  YT K+L+   +SP  GSVP T +P+SF D +W+   P RR+FF++FPH T HF +
Sbjct: 1   MADVDYTAKLLNLYRISPPAGSVPTTLLPISFLDFIWMICRPMRRLFFFQFPHSTNHFMD 60

Query: 61  AILPSLKHSLSLALQQFFPFSANLIFPPQPQKPHILCSPRDSVSLSIAESDSRHFHALIA 120
           ++ P+LK SLSL+LQ FFP + NLI PPQPQKPHIL +P DSV L++ ES    F+ LIA
Sbjct: 61  SVFPTLKSSLSLSLQHFFPLAGNLILPPQPQKPHILYTPHDSVPLTVTESTGGDFNTLIA 120

Query: 121 DHAKDATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHVAADGPA 180
           D+ +DA   Y    D P  R+  DG ++AIP   +++TIFP QGL I ++F HV ADG A
Sbjct: 121 DYPRDAKHFYPFVPDMPESRLLPDG-SRAIPNFAIQITIFPNQGLCICIAFHHVVADGIA 180

Query: 181 FHHFVKSWASICK----GQDFDGPTPVHDRTVVRDSNGLESTILQ--LQW-SMAGPINFG 240
            HHF+KSWASI +       F+GP P H+R  ++D  GLES  LQ    W S+  P+ + 
Sbjct: 181 IHHFIKSWASISRNGGTSDSFEGPLPCHNRAAIKDVKGLESIFLQELSSWDSLWNPVPY- 240

Query: 241 SEPISKRLADMVRATFVLSKAQIEGLKSWARAESPH--------FSSFVAVCSLVWVCFI 300
              I +   D VR T VL++  IE LK+W  AES +         SSF+  CSLVW C I
Sbjct: 241 KPIIDQAPVDRVRTTLVLTRPMIEKLKTWVSAESNNDEELKPLRLSSFIVTCSLVWGCLI 300

Query: 301 KSREGEGGNGKG-SEVCCFAFAADCRKRLGYPMPAGYFGNCLAFCHAVVEKGELLGGDGV 360
           KS + E  + +   E+C F F ADCR RL  P+P  YFGNCL      ++K EL+  +  
Sbjct: 301 KSTQSEVSDSEDEDELCHFGFLADCRNRLKDPLPPTYFGNCLDVGVVELKKSELVKRNST 360

Query: 361 IVGMKAIGKRVGEAEDEPLKETAEWASIWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPI 420
           ++  KAIG RV E E+EPLK   +W S+WK Y +      +AGSP+L AYD DFGWGR +
Sbjct: 361 VMAAKAIGNRVEEFENEPLKVAEKWFSLWKAYSKDKHFVTIAGSPKLRAYDTDFGWGRAM 420

Query: 421 KTDVIHIDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTL 452
           K++V+H+DS    SL +SR+E GGIE+GL L    M RF+A+WEQ L
Sbjct: 421 KSEVLHVDSSESISLQDSRDEEGGIEIGLGLHNTRMDRFMAIWEQNL 465

BLAST of MS004846 vs. ExPASy TrEMBL
Match: A0A6J1G6S5 (coumaroyl-CoA:anthocyanidin 3-O-glucoside-6''-O-coumaroyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111451276 PE=3 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 2.7e-117
Identity = 218/467 (46.68%), Postives = 293/467 (62.74%), Query Frame = 0

Query: 1   MAESVYTVKVLDECLVSPSPGSVPPTHIPLSFFDLMWLRTAPTRRVFFYKFPHPTTHFTN 60
           MA+  YT K+L+   +SP  GSVP T +PLSF D +W+   P RR+FF++FPH T HF +
Sbjct: 39  MADVDYTAKLLNVYRISPPAGSVPTTLLPLSFLDFIWMICRPMRRLFFFQFPHSTNHFMD 98

Query: 61  AILPSLKHSLSLALQQFFPFSANLIFPPQPQKPHILCSPRDSVSLSIAESDSRHFHALIA 120
           ++ P+LK SLSL+LQ FFP + NLI PP P KPHIL +P DSV L++ ES    F+ LIA
Sbjct: 99  SVFPTLKRSLSLSLQHFFPLAGNLILPPPPHKPHILYTPHDSVPLTVTESTGGDFNTLIA 158

Query: 121 DHAKDATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHVAADGPA 180
           D+ +DA   Y    D P   +  DG ++AIP   +++TIFP QGL I ++F HV ADG A
Sbjct: 159 DYPRDAKHFYPFVPDMPESCLLPDG-SRAIPNFAIQITIFPNQGLCICIAFQHVVADGIA 218

Query: 181 FHHFVKSWASICK----GQDFDGPTPVHDRTVVRDSNGLESTILQ--LQW-SMAGPINFG 240
            HHF+KSWASI +       F+GP P H+R  ++D  GLES  LQ    W S+  P+ + 
Sbjct: 219 IHHFIKSWASISRNGGASDSFEGPLPCHNRAAIKDVKGLESIFLQELSSWDSLWNPVPY- 278

Query: 241 SEPISKRLADMVRATFVLSKAQIEGLKSWARAESPH--------FSSFVAVCSLVWVCFI 300
              I +   D VR T VL++  IE LK+W  AES +         SSF+  CSLVW C I
Sbjct: 279 KPIIDQAPVDRVRTTLVLTRPMIEKLKTWVSAESNNDEESKPLRLSSFIVTCSLVWGCLI 338

Query: 301 KSREGEGGNGKGSE-VCCFAFAADCRKRLGYPMPAGYFGNCLAFCHAVVEKGELLGGDGV 360
           KS + E  +    + +C F F ADCR R+  P+P  YFGNCL      ++K EL+  + +
Sbjct: 339 KSTQSEASDSVDEDKLCYFGFLADCRNRVKDPLPPTYFGNCLDVGVVELKKSELVKRNSI 398

Query: 361 IVGMKAIGKRVGEAEDEPLKETAEWASIWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPI 420
           ++  KAIGKRV E E+EPLK   +W S+WK Y +      +AGSP+L AYD DFGWGR +
Sbjct: 399 VMAAKAIGKRVEEFENEPLKVAEKWFSLWKTYSKDKHFVTIAGSPKLKAYDTDFGWGRAM 458

Query: 421 KTDVIHIDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTL 452
           K++V+H+DS    S+ +SR+E GGIE+GL L    M RF+A+WEQ L
Sbjct: 459 KSEVLHVDSSESISVQDSRDEQGGIEIGLGLHNTQMDRFMAIWEQNL 503

BLAST of MS004846 vs. TAIR 10
Match: AT1G03940.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 285.4 bits (729), Expect = 7.9e-77
Identity = 164/465 (35.27%), Postives = 251/465 (53.98%), Query Frame = 0

Query: 9   KVLDECLVSPSPGSVPPTHIPLSFFDLMWLRTAPTRRVFFYKFPHPTTHFTNAILPSLKH 68
           K+++ C +SP  G+VP T +PL+FFD  WL       +FF+ + + T  F    +P+LKH
Sbjct: 9   KIIETCHISPPKGTVPSTTLPLTFFDAPWLSLPLADSLFFFSYQNSTESFLQDFVPNLKH 68

Query: 69  SLSLALQQFFPFSANLIFPPQPQKPHILCSP-RDSVSLSIAESDSRHFHALIADHAKDAT 128
           SLS+ LQ FFP++  LI PP+P  P++  +  +DS+  ++AES    F  L +D  KD +
Sbjct: 69  SLSITLQHFFPYAGKLIIPPRPDPPYLHYNDGQDSLVFTVAESTETDFDQLKSDSPKDIS 128

Query: 129 DVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHVAADGPAFHHFVKS 188
            ++ +    P   V+ +G  Q  P++ ++VTIFP  G+ IG S  HV ADG  F HF+K 
Sbjct: 129 VLHGVLPKLPPPHVSPEG-IQMRPIMAMQVTIFPGAGICIGNSATHVVADGVTFSHFMKY 188

Query: 189 WASICKGQDFDGPT------PVHD-RTVVRDSNGLESTILQLQWSMAGPINFGSEPISKR 248
           W S+ K    D  T      P+H  R +++D   + +  L+  WS     N         
Sbjct: 189 WMSLTKSSGKDPATVLLPSLPIHSCRNMIKDPGEVGAGHLERFWSQ----NSAKHSSHVT 248

Query: 249 LADMVRATFVLSKAQIEGLKSWARAESPH---FSSFVAVCSLVWVCFIKS----REGEGG 308
             +MVRATF LS+ QI+ LKSW   +S +    S+FV   + +WV  IK+     E +  
Sbjct: 249 PENMVRATFTLSRKQIDNLKSWVTEQSENQSPVSTFVVTLAFIWVSLIKTLVQDSETKAN 308

Query: 309 NGKGSEVCCFAFAADCRKRLGY--PMPAGYFGNCLAFCHAVVEKGELLGGDGVIVGMKAI 368
                EV       DCR RL Y  P+P  YFGNC+A     V+K +LLG   V+    AI
Sbjct: 309 EEDKDEVFHLMINVDCRNRLKYTQPIPQTYFGNCMAPGIVSVKKHDLLGEKCVLAASDAI 368

Query: 369 GKRVGEAEDEPLKETA-EWASIWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPIKTDVIH 428
             R+ +     L +TA  W    +K+       ++AG+P+LG YD+DFG G+P K +++H
Sbjct: 369 TARIKDMLSSDLLKTAPRWGQGVRKWVMSHYPTSIAGAPKLGLYDMDFGLGKPCKMEIVH 428

Query: 429 IDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTLNLFS 456
           I++G   +  ESR+ + G+E+G+AL    M  F ++ +Q +  F+
Sbjct: 429 IETGGSIAFSESRDGSNGVEIGIALEKKKMDVFDSILQQGIKKFA 468

BLAST of MS004846 vs. TAIR 10
Match: AT1G03495.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 282.7 bits (722), Expect = 5.2e-76
Identity = 162/469 (34.54%), Postives = 250/469 (53.30%), Query Frame = 0

Query: 1   MAESVYTVKVLDECLVSPSPGSVPPTHIPLSFFDLMWLRTAPTRRVFFYKFPHPTTHFTN 60
           MA  +    +++ C +SP  G+V  T +PL+FFD  WL       +FF+ + + T  F  
Sbjct: 1   MAAQLQPYNIIETCHISPPKGTVASTTLPLTFFDAPWLSLPLADSLFFFSYQNSTESFLQ 60

Query: 61  AILPSLKHSLSLALQQFFPFSANLIFPPQPQKPHI-LCSPRDSVSLSIAESDSRHFHALI 120
             +P+LKHSLS+ LQ FFP++  LI PP+P  P++   +  DS+  ++AES    F  L 
Sbjct: 61  DFVPNLKHSLSITLQHFFPYAGKLIIPPRPDPPYLHYNAGEDSLVFTVAESTETDFDQLK 120

Query: 121 ADHAKDATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHVAADGP 180
           +D  KD + ++ +    P   V+ +G  Q  P++ ++VTIFP  G+ IG S  HV ADG 
Sbjct: 121 SDSPKDISVLHGVLPKLPPPHVSPEG-IQMRPIMAMQVTIFPGAGICIGNSATHVVADGV 180

Query: 181 AFHHFVKSWASICKGQDFDGPT------PVHD-RTVVRDSNGLESTILQLQWSMAGPINF 240
            F HF+K W S+ K    D  T      P+H  R +++D   + +  L+  WS     N 
Sbjct: 181 TFSHFMKYWMSLTKSSGKDPATVLLPSLPIHSCRNIIKDPGEVAAGHLERFWSQ----NS 240

Query: 241 GSEPISKRLADMVRATFVLSKAQIEGLKSWARAESPH---FSSFVAVCSLVWVCFIKSRE 300
                     +MVRATF LS+ QI+ LKSW   +S +    S+FV   + +WV  IK+  
Sbjct: 241 AKHSSHVTPENMVRATFTLSRKQIDNLKSWVTEQSENQSPVSTFVVTLAFIWVSLIKTLV 300

Query: 301 GEGGNGKGSEVCCFAFAADCRKRLGY--PMPAGYFGNCLAFCHAVVEKGELLGGDGVIVG 360
            +       EV       DCR RL Y  P+P  YFGNC+A     V+K +LLG   V+  
Sbjct: 301 QDSETEAKDEVFHLMINVDCRNRLKYTQPIPQTYFGNCMAPGIVSVKKHDLLGEKCVMAA 360

Query: 361 MKAIGKRVGEAEDEPLKETA-EWASIWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPIKT 420
             AI  R+ +     L +TA  W    +K+       ++AG+P+LG YD+DFG G+P K 
Sbjct: 361 SDAITARIKDMLSSDLLKTAPRWGQGVRKWVMSHYPTSIAGAPKLGLYDMDFGLGKPCKM 420

Query: 421 DVIHIDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTLNLFS 456
           +++HI++G   +  ESR+ + G+E+G+AL    M  F ++ ++ +  F+
Sbjct: 421 EIVHIETGGSIAFSESRDGSNGVEIGIALEKKKMDVFDSLLQKGIKKFA 464

BLAST of MS004846 vs. TAIR 10
Match: AT3G29680.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 253.8 bits (647), Expect = 2.6e-67
Identity = 170/456 (37.28%), Postives = 245/456 (53.73%), Query Frame = 0

Query: 16  VSPSPGSVPPTHIPLSFFDLMWLRTAPTRRVFFYKFPHPT-THFTNAILPSLKHSLSLAL 75
           VS    SV P  +PL+FFDL+WL+  P  RV FYK    +   F ++ILP L+ SLSL L
Sbjct: 11  VSLVTNSVEPLVLPLTFFDLLWLKLNPIERVTFYKLTESSRDSFFSSILPKLEQSLSLVL 70

Query: 76  QQFFPFSANLIFPPQPQKPHILCSPRDSVSLSIAESDSRHFHALIADHAKDATDVYQLCQ 135
             F P S +L + PQ  KPHI+  P+D+VSL++ ES++  F  + +   +  T++  L  
Sbjct: 71  SHFLPLSGHLKWNPQDPKPHIVIFPKDTVSLTVVESEA-DFSYISSKELRLETELRPLV- 130

Query: 136 DWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHVAADGPAFHHFVKSWASICK- 195
             P  +V+SD  +    LL+L++T+FP QG SIG +  HV  DG     F KSWA ICK 
Sbjct: 131 --PELQVSSDSAS----LLSLQITLFPNQGFSIGTTVHHVVMDGKTASKFHKSWAHICKH 190

Query: 196 ---GQDFDGPTPVHDRTVVRDSNGLESTILQLQWSMAGPINFG---SEPISKRL-ADMVR 255
               QDFD PT V DRTV+    GLE  I QL   ++   ++    + P +K +  D+VR
Sbjct: 191 GTTPQDFDLPT-VLDRTVINVPAGLEQKIFQLSSYISEEKDYARTLTLPPAKEIDNDVVR 250

Query: 256 ATFVLSKAQIEGLKSWARAESP----HFSSFVAVCSLVWVCFIKSREGEGGNGKGSEVCC 315
            T  L++  IE LK  A+ ES     H S+FV   + V  C +KS  G+      +    
Sbjct: 251 VTLELTEVDIEKLKERAKNESTRSDLHLSTFVVSYAYVLTCMVKSCGGD-----ANRPVR 310

Query: 316 FAFAADCRKRLGYPMPAGYFGNC-LAFCHAVVEKGELLGGDGVIVGMKAIGKRV---GEA 375
           F +AAD R RL  P+P  YFGNC L       +    LG DG + G++ +   V   G  
Sbjct: 311 FMYAADFRNRLDPPVPLTYFGNCVLPIDFNGYKATTFLGKDGYVNGVEILSDSVRGLGSR 370

Query: 376 EDEPLKETAEWASIWKKYKERGVVFAVAGSPRLGAYDVDFGWGRPIKTDVIHIDSGSLFS 435
             E + E  E  +   K   + V   V GS + G Y  DFGWGRP+KTDV+ +   + FS
Sbjct: 371 NIESIWEVYEDGTKNMKLDTQNV--TVTGSNQFGIYGSDFGWGRPVKTDVMSLYKNNEFS 430

Query: 436 LGESRNENGGIEVGLALSTAHMARFVAVWEQTLNLF 455
           +   R+E GG+E+G++L    M  F++++    +++
Sbjct: 431 MSARRDEIGGLEIGISLKKCEMNVFLSLFTSDFDIY 450

BLAST of MS004846 vs. TAIR 10
Match: AT3G29670.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 243.8 bits (621), Expect = 2.7e-64
Identity = 156/462 (33.77%), Postives = 237/462 (51.30%), Query Frame = 0

Query: 7   TVKVLDECLVSPSPGSVPPT----HIPLSFFDLMWLRTAPTRRVFFYKFPHPT-THFTNA 66
           T+ V++   V+P+  SV  +     +PL+FFDL WL   P +RVFFY+    T  HF + 
Sbjct: 2   TLHVIETARVTPTDYSVINSANLHKLPLTFFDLPWLLFQPVKRVFFYELTESTRDHFHSI 61

Query: 67  ILPSLKHSLSLALQQFFPFSANLIFPPQPQKPHILCSPRDSVSLSIAESDSRHFHALIAD 126
           ILP LK SLSL L+ + P + ++ + P   KP I+ S    V ++IAESD+   H L   
Sbjct: 62  ILPKLKDSLSLILRNYLPLTGHITWEPNEPKPSIIVSENGVVLVTIAESDADFSH-LSGY 121

Query: 127 HAKDATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHVAADGPAF 186
             +  ++++ L    P   V+ D  T      ++++T+FP QG SIG++  H   DG   
Sbjct: 122 GQRPLSELHALV---PKLPVSDDSAT----AFSIQITLFPNQGFSIGVAAHHAVLDGKTS 181

Query: 187 HHFVKSWASICKGQDFDGP---TPVHDRTVVRDSNGLESTILQLQWSM----AGPINFGS 246
             F+K+WA ICK +    P   TP +DR++++    L+  +++L  S+        +  S
Sbjct: 182 STFIKAWAQICKQELQSMPENLTPSYDRSLIKYPTYLDEKMIELVRSLKEDQTNIRSLTS 241

Query: 247 EPISKRLADMVRATFVLSKAQIEGLKSWARAESP--HFSSFVAVCSLVWVCFIKSREGEG 306
            P SK   D+V AT VLS+A IE L+   +  SP  H S+FV   +  W CF+K+R   G
Sbjct: 242 LPSSKLGDDVVLATLVLSRADIERLREQVKNVSPSLHLSTFVIAYAYAWTCFVKAR---G 301

Query: 307 GNGKGSEVCCFAFAADCRKRLGYPMPAGYFGNCLAFCHAVVEK-GELLGGDGVIVGMKAI 366
           GN   S      F  D R RL   +P  YFGNC+        K  E +   G +   + I
Sbjct: 302 GNKDRS--VSLLFVGDFRDRLDPKLPGTYFGNCMIPVGCYNRKAAEFMEEKGFVTAAEII 361

Query: 367 GKRVGEAEDEPLKETAEWASIWKKYKERGVVF-AVAGSPRLGAYDVDFGWGRPIKTDVIH 426
              V       ++  A+       ++     F  +AGS RLG Y+ DFGWGRP+K D++ 
Sbjct: 362 SDLVKGLSSRKIETIADTFVEGFSFQSWSTQFGTIAGSTRLGVYEADFGWGRPVKVDIVS 421

Query: 427 IDSGSLFSLGESRNENGGIEVGLALSTAHMARFVAVWEQTLN 453
           ID G   ++ E R+E+GG+E+G+ L    M   V+ +   L+
Sbjct: 422 IDQGEAIAMAERRDESGGVEIGMCLKKTEMDSVVSFFNNGLH 450

BLAST of MS004846 vs. TAIR 10
Match: AT5G39050.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 233.4 bits (594), Expect = 3.6e-61
Identity = 149/461 (32.32%), Postives = 226/461 (49.02%), Query Frame = 0

Query: 7   TVKVLDECLVSPS-PGSVPPTHIPLSFFDLMWLRTAPTRRVFFYKFPHPT-THFTNAILP 66
           ++KV+D   V+PS   S     +PL+FFDL+W +     RV FYK    +   F + I+P
Sbjct: 9   SLKVIDVARVTPSNSDSSESLTLPLTFFDLLWYKLHAVERVIFYKLTDASRPFFDSVIVP 68

Query: 67  SLKHSLSLALQQFFPFSANLIFPPQPQKPHILCSPRDSVSLSIAESDSRHFHALIADHAK 126
           +LK SLS +L  + P +  L++ P   KP I+ +P D+VS ++AES++  F  L      
Sbjct: 69  NLKTSLSSSLSHYLPLAGKLVWEPLDPKPKIVYTPNDAVSFTVAESNA-DFSRLTGKEPF 128

Query: 127 DATDVYQLCQDWPNFRVASDGTTQAIPLLTLRVTIFPYQGLSIGLSFLHVAADGPAFHHF 186
             T++Y L    P   V+ D  +     ++ +VT+FP QG  I ++  H   DG    +F
Sbjct: 129 PTTELYPLV---PELHVSDDSAS----AVSFQVTLFPNQGFCISVNAHHAVLDGKTTTNF 188

Query: 187 VKSWASICKGQDFDGP---TPVHDRTVVRDSNGLESTILQLQWSMAGPINFGSEPISKRL 246
           +KSWA  CK QD   P    PV+DRTV++D   L++ IL     +A     G EP + + 
Sbjct: 189 LKSWARTCKNQDSFLPQDLIPVYDRTVIKDPMDLDTKILNAWHRVAKVFTGGKEPENPKS 248

Query: 247 ----------ADMVRATFVLSKAQIEGLKSWARAESP-----------HFSSFVAVCSLV 306
                      D+ R T  L++  I+ L+   + ES              S+FV V S  
Sbjct: 249 LKLLWSPEIGPDVFRYTLNLTREDIQKLRERLKKESSSSSVSSSPKELRLSTFVIVYSYA 308

Query: 307 WVCFIKSREGEGGNGKGSEVCCFAFAADCRKRLGYPMPAGYFGNCLAFCHAVVEKGE-LL 366
             C IK+R G+     G     + FA DCR  +  P+P+ YFGNC++ C  +    E  +
Sbjct: 309 LTCLIKARGGDPSRPVG-----YGFAVDCRSLMVPPVPSSYFGNCVSACFKMSLTAETFM 368

Query: 367 GGDGVIVGMKAIGKRVGEAEDEPLKETAEWASIWKKYKERGVVFAVAGSPRLGAYDVDFG 426
             +G +   + +   V   ++    +  E    +        V +VAGS R G Y +DFG
Sbjct: 369 SEEGFLAAARMVSDSVEALDENVALKIPEILEGFTTLSPGTQVLSVAGSTRFGVYGLDFG 428

Query: 427 WGRPIKTDVIHIDSGSLFSLGESRNENGGIEVGLALSTAHM 441
           WGRP K  V+ ID G   S  ESR+ +GG+E+G +L    M
Sbjct: 429 WGRPEKVVVVSIDQGEAISFAESRDGSGGVELGFSLKKHEM 456