MS004829 (gene) Bitter gourd (TR) v1

BLAST of MS004829 vs. NCBI nr
Match: XP_022140348.1 (indole-3-acetic acid-amido synthetase GH3.10 [Momordica charantia])

HSP 1 Score: 1234.6 bits (3193), Expect = 0.0e+00
Identity = 611/614 (99.51%), Postives = 612/614 (99.67%), Query Frame = 0

Query: 1   MEAEAEAGGAGNDHGELSEKEQEIIDWFEQVTSDAGVAQSRTLRQILQQNCGVEYLKPWL 60
           MEAEAEAGGAGNDHGELSEKEQEIIDWFEQVTSDAGVAQSRTLRQILQQNCGVEYLKPWL
Sbjct: 1   MEAEAEAGGAGNDHGELSEKEQEIIDWFEQVTSDAGVAQSRTLRQILQQNCGVEYLKPWL 60

Query: 61  GDVNIHQIQDDDSALESLFTSSVPLSSHPHFEPFLQRIADGDSAPLLTQLPIKTLSLSSG 120
           GDVNIHQIQDDDSALESLFTSSVPLSSHPHFEPFLQRIADGDS PLLTQLPIKTLSLSSG
Sbjct: 61  GDVNIHQIQDDDSALESLFTSSVPLSSHPHFEPFLQRIADGDSTPLLTQLPIKTLSLSSG 120

Query: 121 TTEGRQKYVPFTPHSAQTTLLIFRLAAAYRSRVYPIREGGKIVEFIYSSKQTKTKGGITT 180
           TTEGRQKYV FTPHSAQTTLLIFRLAAAYRSRVYPIREGGKIVEFIYSSKQTKTKGGITT
Sbjct: 121 TTEGRQKYVLFTPHSAQTTLLIFRLAAAYRSRVYPIREGGKIVEFIYSSKQTKTKGGITT 180

Query: 181 GTATTHYYASEEFKIKQLKTQSFTCSPEEVISGGDYKQSTYCHLLLGLFFAHQVEFVTST 240
           GTATTHYYASEEFKIKQLKTQSFTCSPEEVISGGDYKQSTYCHLLLGLFFAHQVEFVTST
Sbjct: 181 GTATTHYYASEEFKIKQLKTQSFTCSPEEVISGGDYKQSTYCHLLLGLFFAHQVEFVTST 240

Query: 241 FAYTILQAFNQLEEVWPDICRDITHASLSSTVTIPKMRRAVLDIISPNPSLASSIRASCQ 300
           FAYTILQAFNQLEEVWPDICRDITHASLSSTVTIPKMRRAVLDIISPNPSLASSIRASCQ
Sbjct: 241 FAYTILQAFNQLEEVWPDICRDITHASLSSTVTIPKMRRAVLDIISPNPSLASSIRASCQ 300

Query: 301 RLRARDWFGLIPELWPNAKYVYSITTGSMQPYVKKLRHYAAGLPLVSADYGSTESWIGVN 360
           RLRARDWFGLIPELWPNAKYVYSITTGSMQPYVKKLRHYAAGLPLVSADYGSTESWIGVN
Sbjct: 301 RLRARDWFGLIPELWPNAKYVYSITTGSMQPYVKKLRHYAAGLPLVSADYGSTESWIGVN 360

Query: 361 VDPSLPPENVTFAVVPTFSYFEFIPLFRQHQNQNQICSSDFDIGVDDFLEGEPVPLSRVK 420
           VDPSLPPENVTFAVVPTFSYFEFIPLFRQHQNQNQICSSDFDIGVDDFLEGEPVPLSRVK
Sbjct: 361 VDPSLPPENVTFAVVPTFSYFEFIPLFRQHQNQNQICSSDFDIGVDDFLEGEPVPLSRVK 420

Query: 421 IGQQYEVVLTTFTGLYRCRLGDIVEVSGFHNKTPKLSFICRRKLVLTINIDKNTEKDLQM 480
           IGQQYEVVLTTFTGLYRCRLGDIVEVSGFHNKTPKLSFICRRKLVLTINIDKNTEKDLQM
Sbjct: 421 IGQQYEVVLTTFTGLYRCRLGDIVEVSGFHNKTPKLSFICRRKLVLTINIDKNTEKDLQM 480

Query: 481 VVERGAQLLGDKSQSGAEVVDFTSHADVADQPGHYVIFWEVKGDVEDAVLGECCTLMDAA 540
           VVERGAQLLGDKSQSGAEVVDFTSHADVADQPGHYVIFWEVKGDVEDAVLGECCTLMDAA
Sbjct: 481 VVERGAQLLGDKSQSGAEVVDFTSHADVADQPGHYVIFWEVKGDVEDAVLGECCTLMDAA 540

Query: 541 FVDYGYVVSRRSNSIGPLELRVVERGTFKKILEHCIGNGGALSQFKTPRCTTNQLLLSIL 600
           FVD+GYVVSRRSNSIGPLELRVVERGTFKKILEHCIGNGGALSQFKTPRCTTNQLLLSIL
Sbjct: 541 FVDHGYVVSRRSNSIGPLELRVVERGTFKKILEHCIGNGGALSQFKTPRCTTNQLLLSIL 600

Query: 601 NLSTVKTFRSTAYA 615
           NLSTVKTFRSTAYA
Sbjct: 601 NLSTVKTFRSTAYA 614

BLAST of MS004829 vs. NCBI nr
Match: XP_022986272.1 (indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita maxima])

HSP 1 Score: 996.9 bits (2576), Expect = 7.7e-287
Identity = 480/595 (80.67%), Postives = 540/595 (90.76%), Query Frame = 0

Query: 19  EKEQEIIDWFEQVTSDAGVAQSRTLRQILQQNCGVEYLKPWLGDVNIHQIQDDDSALESL 78
           E+ ++++DWFE+V+S+AGVAQSRTLR+IL+QNCGVEYL  WLGDVNIHQI  DD ALESL
Sbjct: 3   EEVEDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQI-PDDFALESL 62

Query: 79  FTSSVPLSSHPHFEPFLQRIADGDSAPLLTQLPIKTLSLSSGTTEGRQKYVPFTPHSAQT 138
           FTS VPLSSH  FEPFLQRIADGDS+PLLTQ PI+TLSLSSGTTEGRQKYVPFTPHSAQT
Sbjct: 63  FTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQT 122

Query: 139 TLLIFRLAAAYRSRVYPIREGGKIVEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQL 198
           TLLIFR+AAAYRSRVYP REGG+I+EFIYSSKQTKTKGGITTGTATTHYYASE+FKIKQL
Sbjct: 123 TLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQL 182

Query: 199 KTQSFTCSPEEVISGGDYKQSTYCHLLLGLFFAHQVEFVTSTFAYTILQAFNQLEEVWPD 258
           KT+SFTCSP+EVISGGDYKQSTYCHLLLGLFF+ QVEFVTSTFAYTI+QAFN+LE+ WP 
Sbjct: 183 KTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQ 242

Query: 259 ICRDITHASLSSTVTIPKMRRAVLDIISPNPSLASSIRASCQRLRARDWFGLIPELWPNA 318
           IC DITHA+L S +T+P++R+AVL+IISPNP L + I+A C+ L  +DW  L+P+LW N 
Sbjct: 243 ICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSNC 302

Query: 319 KYVYSITTGSMQPYVKKLRHYAAGLPLVSADYGSTESWIGVNVDPSLPPENVTFAVVPTF 378
           KYVYSI TGSMQPY+KKLR YA GLPLVS DYGSTESWIG+NVDP LPPENVTFAVVPTF
Sbjct: 303 KYVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPTF 362

Query: 379 SYFEFIPLFRQHQNQNQICSSDFDIGVDDFLEGEPVPLSRVKIGQQYEVVLTTFTGLYRC 438
           SYFEFIPLFRQHQ  +  C S      DDFLEG+P+PLS+VKIGQQYE+VLTTFTGLYRC
Sbjct: 363 SYFEFIPLFRQHQQHD--CGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRC 422

Query: 439 RLGDIVEVSGFHNKTPKLSFICRRKLVLTINIDKNTEKDLQMVVERGAQLLGDKSQSGAE 498
           RLGD+VEV+GFHNKTPKL+FICRRKLVLT+NIDKNTEKDLQ+VVERG+QLL D  QSGAE
Sbjct: 423 RLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLAD--QSGAE 482

Query: 499 VVDFTSHADVADQPGHYVIFWEVKGDVEDAVLGECCTLMDAAFVDYGYVVSRRSNSIGPL 558
           +VDFTSHAD+ +QPGHYVIFWEVKG+V+DAVLG CC+ MDAAFVDYGYVVSRRSN+IGPL
Sbjct: 483 LVDFTSHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPL 542

Query: 559 ELRVVERGTFKKILEHCIGNGGALSQFKTPRCTTNQLLLSILNLSTVKTFRSTAY 614
           ELR+VERG+F KI+EH IGNG ALSQFKTPRCTTNQ+LL+ILNLSTVK F STAY
Sbjct: 543 ELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 592

BLAST of MS004829 vs. NCBI nr
Match: KAG6570848.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 993.8 bits (2568), Expect = 6.5e-286
Identity = 480/595 (80.67%), Postives = 537/595 (90.25%), Query Frame = 0

Query: 19  EKEQEIIDWFEQVTSDAGVAQSRTLRQILQQNCGVEYLKPWLGDVNIHQIQDDDSALESL 78
           E+ ++++DWFE+V+S+AGVAQ RTLR+IL+QNCGVEYL  WLGDVNIHQI  DD ALESL
Sbjct: 3   EEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQI-PDDFALESL 62

Query: 79  FTSSVPLSSHPHFEPFLQRIADGDSAPLLTQLPIKTLSLSSGTTEGRQKYVPFTPHSAQT 138
           FTS +PLSSH  FEPFLQRIADGDS+PLLTQ PI+TLSLSSGTTEGRQKYVPFTPHSAQT
Sbjct: 63  FTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQT 122

Query: 139 TLLIFRLAAAYRSRVYPIREGGKIVEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQL 198
           TLLIFR+AAAYRSRVYP REGG+I+EFIYSSKQTKTKGGITTGTATTHYYASE+FKIKQL
Sbjct: 123 TLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQL 182

Query: 199 KTQSFTCSPEEVISGGDYKQSTYCHLLLGLFFAHQVEFVTSTFAYTILQAFNQLEEVWPD 258
           KT+SFTCSP+EVISGGDYKQSTYCHLLLGLFF+ QVEFVTSTFAYTI+QAFN+LE+ WP 
Sbjct: 183 KTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQ 242

Query: 259 ICRDITHASLSSTVTIPKMRRAVLDIISPNPSLASSIRASCQRLRARDWFGLIPELWPNA 318
           IC DITHA+L S +T+P++R+AVL+IISPNP L + I A C+ L  +DW  L+P+LWPN 
Sbjct: 243 ICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNC 302

Query: 319 KYVYSITTGSMQPYVKKLRHYAAGLPLVSADYGSTESWIGVNVDPSLPPENVTFAVVPTF 378
           KYVYSI TGSMQPY+KKLR YA  LPLVS DYGSTESWIGVNVDP LPPENVTFAVVPTF
Sbjct: 303 KYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTF 362

Query: 379 SYFEFIPLFRQHQNQNQICSSDFDIGVDDFLEGEPVPLSRVKIGQQYEVVLTTFTGLYRC 438
           SYFEFIPLFRQHQ  +  C S      DDFLEG+P+PLS+VKIGQQYE+VLTTFTGLYRC
Sbjct: 363 SYFEFIPLFRQHQQHD--CGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRC 422

Query: 439 RLGDIVEVSGFHNKTPKLSFICRRKLVLTINIDKNTEKDLQMVVERGAQLLGDKSQSGAE 498
           RLGD+VEV+GFHNKTPKL+FICRRKLVLT+NIDKNTEKDLQ+VVERG+QLL D  QSGAE
Sbjct: 423 RLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLAD--QSGAE 482

Query: 499 VVDFTSHADVADQPGHYVIFWEVKGDVEDAVLGECCTLMDAAFVDYGYVVSRRSNSIGPL 558
           +VDFTSHAD+ +QPGHYVIFWEVKG VEDAVLG CC+ MDAAFVDYGYVVSRRSN+IGPL
Sbjct: 483 LVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPL 542

Query: 559 ELRVVERGTFKKILEHCIGNGGALSQFKTPRCTTNQLLLSILNLSTVKTFRSTAY 614
           ELR+VERG+F KI+EH IGNG ALSQFKTPRCTTNQ+LL+ILNLSTVK F STAY
Sbjct: 543 ELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 592

BLAST of MS004829 vs. NCBI nr
Match: XP_022943441.1 (indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita moschata])

HSP 1 Score: 993.4 bits (2567), Expect = 8.5e-286
Identity = 480/595 (80.67%), Postives = 536/595 (90.08%), Query Frame = 0

Query: 19  EKEQEIIDWFEQVTSDAGVAQSRTLRQILQQNCGVEYLKPWLGDVNIHQIQDDDSALESL 78
           E  ++++DWFE+V+S+AGVAQ RTLR+IL+QNCGVEYL  WLGDVNIHQI  DD ALESL
Sbjct: 3   EDVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQI-PDDFALESL 62

Query: 79  FTSSVPLSSHPHFEPFLQRIADGDSAPLLTQLPIKTLSLSSGTTEGRQKYVPFTPHSAQT 138
           FTS +PLSSH  FEPFLQRIADGDS+PLLTQ PI+TLSLSSGTTEGRQKYVPFTPHSAQT
Sbjct: 63  FTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQT 122

Query: 139 TLLIFRLAAAYRSRVYPIREGGKIVEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQL 198
           TLLIFR+AAAYRSRVYP REGG+I+EFIYSSKQTKTKGGITTGTATTHYYASE+FKIKQL
Sbjct: 123 TLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQL 182

Query: 199 KTQSFTCSPEEVISGGDYKQSTYCHLLLGLFFAHQVEFVTSTFAYTILQAFNQLEEVWPD 258
           KT+SFTCSP+EVISGGDYKQSTYCHLLLGLFF+ QVEFVTSTFAYTI+QAFN+LE+ WP 
Sbjct: 183 KTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQ 242

Query: 259 ICRDITHASLSSTVTIPKMRRAVLDIISPNPSLASSIRASCQRLRARDWFGLIPELWPNA 318
           IC DITHA+L S +T+P++R+AVL+IISPNP L + I A C+ L  +DW  L+P+LWPN 
Sbjct: 243 ICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNC 302

Query: 319 KYVYSITTGSMQPYVKKLRHYAAGLPLVSADYGSTESWIGVNVDPSLPPENVTFAVVPTF 378
           KYVYSI TGSMQPY+KKLR YA  LPLVS DYGSTESWIGVNVDP LPPENVTFAVVPTF
Sbjct: 303 KYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTF 362

Query: 379 SYFEFIPLFRQHQNQNQICSSDFDIGVDDFLEGEPVPLSRVKIGQQYEVVLTTFTGLYRC 438
           SYFEFIPLFRQHQ  +  C S      DDFLEG+P+PLS+VKIGQQYE+VLTTFTGLYRC
Sbjct: 363 SYFEFIPLFRQHQQHD--CGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRC 422

Query: 439 RLGDIVEVSGFHNKTPKLSFICRRKLVLTINIDKNTEKDLQMVVERGAQLLGDKSQSGAE 498
           RLGD+VEV+GFHNKTPKL+FICRRKLVLT+NIDKNTEKDLQ+VVERG+QLL D  QSGAE
Sbjct: 423 RLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLAD--QSGAE 482

Query: 499 VVDFTSHADVADQPGHYVIFWEVKGDVEDAVLGECCTLMDAAFVDYGYVVSRRSNSIGPL 558
           +VDFTSHAD+ +QPGHYVIFWEVKG VEDAVLG CC+ MDAAFVDYGYVVSRRSN+IGPL
Sbjct: 483 LVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPL 542

Query: 559 ELRVVERGTFKKILEHCIGNGGALSQFKTPRCTTNQLLLSILNLSTVKTFRSTAY 614
           ELR+VERG+F KI+EH IGNG ALSQFKTPRCTTNQ+LL+ILNLSTVK F STAY
Sbjct: 543 ELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 592

BLAST of MS004829 vs. NCBI nr
Match: XP_023512179.1 (indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 992.6 bits (2565), Expect = 1.5e-285
Identity = 480/595 (80.67%), Postives = 537/595 (90.25%), Query Frame = 0

Query: 19  EKEQEIIDWFEQVTSDAGVAQSRTLRQILQQNCGVEYLKPWLGDVNIHQIQDDDSALESL 78
           E+ ++++DWFE+V+S+AGVAQ RTLR+IL+QNCGVEYL  WLGDVNIHQI  DD ALESL
Sbjct: 3   EEVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQI-PDDFALESL 62

Query: 79  FTSSVPLSSHPHFEPFLQRIADGDSAPLLTQLPIKTLSLSSGTTEGRQKYVPFTPHSAQT 138
           FTS VPLSSH  FEPFLQRIADGDS+PLLTQ PI+TLSLSSGTTEGRQKYVPFTPHSAQT
Sbjct: 63  FTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQT 122

Query: 139 TLLIFRLAAAYRSRVYPIREGGKIVEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQL 198
           TLLIFR+AAAYRSRVYP REGG+I+EFIYSSKQTKTKGGITTGTATTHYYASE+FKIKQL
Sbjct: 123 TLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQL 182

Query: 199 KTQSFTCSPEEVISGGDYKQSTYCHLLLGLFFAHQVEFVTSTFAYTILQAFNQLEEVWPD 258
           KT+SFTCSP+EVISGGDYKQSTYCHLLLGLFF+ QVEFVTSTFAYTI+QAFN+LE+ WP 
Sbjct: 183 KTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQ 242

Query: 259 ICRDITHASLSSTVTIPKMRRAVLDIISPNPSLASSIRASCQRLRARDWFGLIPELWPNA 318
           IC DITHA+L S +T+P++R+AVL+IISPNP L + I A C+ L  +DW  L+P+LWPN 
Sbjct: 243 ICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNC 302

Query: 319 KYVYSITTGSMQPYVKKLRHYAAGLPLVSADYGSTESWIGVNVDPSLPPENVTFAVVPTF 378
           KYVYSI TGS+QPY+KKLR YA  LPLVS DYGSTESWIGVNVDP LPPENVTFAVVPTF
Sbjct: 303 KYVYSIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTF 362

Query: 379 SYFEFIPLFRQHQNQNQICSSDFDIGVDDFLEGEPVPLSRVKIGQQYEVVLTTFTGLYRC 438
           SYFEFIPLFRQHQ  +  C S      DDFLEG+P+PLS+VKIGQQYE+VLTTFTGLYRC
Sbjct: 363 SYFEFIPLFRQHQQHD--CGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRC 422

Query: 439 RLGDIVEVSGFHNKTPKLSFICRRKLVLTINIDKNTEKDLQMVVERGAQLLGDKSQSGAE 498
           RLGD+VEV+GFHNKTPKL+FICRRKLVLT+NIDKNTEKDLQ+VVERG+QLL D  QSGAE
Sbjct: 423 RLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLAD--QSGAE 482

Query: 499 VVDFTSHADVADQPGHYVIFWEVKGDVEDAVLGECCTLMDAAFVDYGYVVSRRSNSIGPL 558
           +VDFTSHAD+ +QPGHYVIFWEVKG VEDAVLG CC+ MDAAFVDYGYVVSRRSN+IGPL
Sbjct: 483 LVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPL 542

Query: 559 ELRVVERGTFKKILEHCIGNGGALSQFKTPRCTTNQLLLSILNLSTVKTFRSTAY 614
           ELR+VERG+F KI+EH IGNG ALSQFKTPRCTTNQ+LL+ILNLSTVK F STAY
Sbjct: 543 ELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 592

BLAST of MS004829 vs. ExPASy Swiss-Prot
Match: Q9ZNS2 (Indole-3-acetic acid-amido synthetase GH3.10 OS=Arabidopsis thaliana OX=3702 GN=GH3.10 PE=2 SV=1)

HSP 1 Score: 823.5 bits (2126), Expect = 1.5e-237
Identity = 403/592 (68.07%), Postives = 480/592 (81.08%), Query Frame = 0

Query: 23  EIIDWFEQVTSDAGVAQSRTLRQILQQNCGVEYLKPWLGDVNIHQIQDDDSALESLFTSS 82
           ++I WFE V+ +A   QS TLR+IL+ N GVEYL+ WLG V++ ++  DD  LE+LFTS 
Sbjct: 10  DVIGWFEHVSENACKVQSETLRRILELNSGVEYLRKWLGTVDVEKM--DDYTLETLFTSL 69

Query: 83  VPLSSHPHFEPFLQRIADGDSAPLLTQLPIKTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 142
           VP+ SH   +P++QRIADG+++PLLTQ PI  LSLSSGTTEGRQKYVPFT HSAQTTL I
Sbjct: 70  VPIVSHADLDPYIQRIADGETSPLLTQEPITVLSLSSGTTEGRQKYVPFTRHSAQTTLQI 129

Query: 143 FRLAAAYRSRVYPIREGGKIVEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQLKTQS 202
           FRL+AAYRSR YPIREGG+I+EFIY+ K+ KT GG+T GTATTHYYASEEFK KQ  T+S
Sbjct: 130 FRLSAAYRSRFYPIREGGRILEFIYAGKEFKTLGGLTVGTATTHYYASEEFKTKQETTKS 189

Query: 203 FTCSPEEVISGGDYKQSTYCHLLLGLFFAHQVEFVTSTFAYTILQAFNQLEEVWPDICRD 262
           FTCSP+EVISGGD+ Q TYCHLLLGL ++ QVEFV S F+YTI+QAF+  EE+W +IC D
Sbjct: 190 FTCSPQEVISGGDFGQCTYCHLLLGLHYSSQVEFVASAFSYTIVQAFSFFEEIWREICAD 249

Query: 263 ITHASLSSTVTIPKMRRAVLDIISPNPSLASSIRASCQRLRAR-DWFGLIPELWPNAKYV 322
           I   +LSS +T+PKMR+AVL +I PNPSLAS I   C  L     WFGLI +LWPNAK++
Sbjct: 250 IKEGNLSSRITLPKMRKAVLALIRPNPSLASHIEEICLELETNLGWFGLISKLWPNAKFI 309

Query: 323 YSITTGSMQPYVKKLRHYAAGLPLVSADYGSTESWIGVNVDPSLPPENVTFAVVPTFSYF 382
            SI TGSM PY+ KLRHYA GLPLVSADYGSTESWIGVNVDP LPPE+V+FAV+PTFSYF
Sbjct: 310 SSIMTGSMLPYLNKLRHYAGGLPLVSADYGSTESWIGVNVDPHLPPEDVSFAVIPTFSYF 369

Query: 383 EFIPLFRQHQNQNQICSSDFDIGVDDFLEGEPVPLSRVKIGQQYEVVLTTFTGLYRCRLG 442
           EFIPL+R+ QNQ+ IC         DF+E +PVPLS+VK+GQ+YE+VLTTFTGLYR RLG
Sbjct: 370 EFIPLYRR-QNQSDICID------GDFVEDKPVPLSQVKLGQEYELVLTTFTGLYRYRLG 429

Query: 443 DIVEVSGFHNKTPKLSFICRRKLVLTINIDKNTEKDLQMVVERGAQLLGDKSQSGAEVVD 502
           D+VEV+ FH  TPKLSFI RRKL+LTINIDKNTEKDLQ VV++ +QLL   ++  AEVVD
Sbjct: 430 DVVEVTSFHKGTPKLSFIYRRKLILTINIDKNTEKDLQRVVDKASQLLSRSTR--AEVVD 489

Query: 503 FTSHADVADQPGHYVIFWEVKGDVEDAVLGECCTLMDAAFVDYGYVVSRRSNSIGPLELR 562
           FTSHADV  +PGHYVI+WE++G+ +D  L ECC  MD AFVDYGYVVSRR NSIGPLELR
Sbjct: 490 FTSHADVIARPGHYVIYWEIRGEADDKALEECCREMDTAFVDYGYVVSRRMNSIGPLELR 549

Query: 563 VVERGTFKKILEHCIGNGGALSQFKTPRCTTNQLLLSILNLSTVKTFRSTAY 614
           VVERGTF K+ E C+G  G L+QFKTPRCTTN ++L ILN ST+K FRS+AY
Sbjct: 550 VVERGTFGKVAERCVGKCGGLNQFKTPRCTTNSVMLDILNDSTIKRFRSSAY 590

BLAST of MS004829 vs. ExPASy Swiss-Prot
Match: A0A1J6KGJ9 (Jasmonoyl--L-amino acid synthetase JAR4 OS=Nicotiana attenuata OX=49451 GN=JAR4 PE=1 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 7.0e-174
Identity = 302/594 (50.84%), Postives = 412/594 (69.36%), Query Frame = 0

Query: 22  QEIIDWFEQVTSDAGVAQSRTLRQILQQNCGVEYLKPWLGDVNIHQIQDDDSALESLFTS 81
           +E+I+ FE +T DAG  Q  TL++IL++N G EYL+ W     ++   D  S     F +
Sbjct: 14  EEVIEEFEVLTKDAGKIQEETLQKILEENGGTEYLQQW----GLNGKTDSLS-----FKN 73

Query: 82  SVPLSSHPHFEPFLQRIADGDSAPLLTQLPIKTLSLSSGTTEGRQKYVPFTPHSAQTTLL 141
            +P+ +H   EP++ RIADGD +P+LT  PI T+SLSSGTT+G+ K+VPF     ++T+ 
Sbjct: 74  CIPIVTHKDLEPYIHRIADGDLSPILTGKPITTISLSSGTTQGKPKFVPFNEELMESTMQ 133

Query: 142 IFRLAAAYRSRVYPIREGGKIVEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQLKTQ 201
           IF+ +  +R+R +P+   GK ++FIY SKQ KTKGG+  GTATT+ Y + +FK      Q
Sbjct: 134 IFKTSFVFRNREFPV-VNGKALQFIYGSKQFKTKGGLAAGTATTNVYRNAQFKKTMKAMQ 193

Query: 202 SFTCSPEEVISGGDYKQSTYCHLLLGLFFAHQVEFVTSTFAYTILQAFNQLEEVWPDICR 261
           +  CSP+EVI G D++QS YCHLL GL F  +V+ V+STFA++I+ AF   E++W ++  
Sbjct: 194 TPCCSPDEVIFGPDFQQSLYCHLLCGLIFRDEVQVVSSTFAHSIVHAFRNFEQIWQELVT 253

Query: 262 DITHASLSSTVTIPKMRRAVLDIISPNPSLASSIRASCQRLRARDWFGLIPELWPNAKYV 321
           +I    LSS V +P MR A+  ++ P+P LA +I   C RL   +W+GLIPEL+PN +Y+
Sbjct: 254 NIREGVLSSRVIVPSMRAAMSKLLKPDPELADTIFNKCSRL--SNWYGLIPELFPNTRYI 313

Query: 322 YSITTGSMQPYVKKLRHYAAGLPLVSADYGSTESWIGVNVDPSLPPENVTFAVVPTFSYF 381
           Y I TGSM+PY+KKLRHYA  LPL+SADYGS+E WIG NV+P LPPE VT+AV+P   YF
Sbjct: 314 YGIMTGSMEPYLKKLRHYAGDLPLLSADYGSSEGWIGANVNPELPPELVTYAVLPNIGYF 373

Query: 382 EFIPLFRQHQNQNQICSSDFDIGVDDFLEGEPVPLSRVKIGQQYEVVLTTFTGLYRCRLG 441
           EFIPL                    D LE  PV L+ VK+G++YE+V+T F GLYR RLG
Sbjct: 374 EFIPLMENL----------------DGLEPMPVGLTEVKLGEEYEIVVTNFAGLYRYRLG 433

Query: 442 DIVEVSGFHNKTPKLSFICRRKLVLTINIDKNTEKDLQMVVERGAQLLGDKSQSGAEVVD 501
           D+V++ GFHN TP+L FICRR L+L+INIDKNTEKDLQ+ VE  A++L D+     EVVD
Sbjct: 434 DVVKIKGFHNGTPELQFICRRNLLLSINIDKNTEKDLQLAVEAAAKILSDEK---LEVVD 493

Query: 502 FTSHADVADQPGHYVIFWEVKGDVEDAVLGECCTLMDAAFVDYGYVVSRRSNSIGPLELR 561
           FTSH +V+  PGHYVIFWE+ G+  + +L ECC  +D +FVD GYV SR+ ++IG LELR
Sbjct: 494 FTSHVNVSADPGHYVIFWELNGEASEEILKECCNCLDKSFVDAGYVGSRKVHAIGALELR 553

Query: 562 VVERGTFKKILEHCIGNGGALSQFKTPRCT--TNQLLLSILNLSTVKTFRSTAY 614
           +V+RGTF KIL+H +G G A+SQFKTPRC   TN  +L IL+ + V+++ STA+
Sbjct: 554 IVKRGTFHKILDHFVGLGAAVSQFKTPRCVGPTNLSVLQILSSNVVESYFSTAF 576

BLAST of MS004829 vs. ExPASy Swiss-Prot
Match: A0A314KSQ4 (Jasmonoyl--L-amino acid synthetase JAR6 OS=Nicotiana attenuata OX=49451 GN=JAR6 PE=1 SV=2)

HSP 1 Score: 611.3 bits (1575), Expect = 1.2e-173
Identity = 308/595 (51.76%), Postives = 415/595 (69.75%), Query Frame = 0

Query: 21  EQEIIDWFEQVTSDAGVAQSRTLRQILQQNCGVEYLKPWLGDVNIHQIQDDDSALESLFT 80
           ++++I+ FE +T DAG  Q  TL++IL+QN G EYL+ W     ++   D  +     F 
Sbjct: 14  QEKVIEEFEDLTKDAGKIQEETLKKILEQNGGTEYLQLW----GLNGRTDPQT-----FK 73

Query: 81  SSVPLSSHPHFEPFLQRIADGDSAPLLTQLPIKTLSLSSGTTEGRQKYVPFTPHSAQTTL 140
           + VP+ +H   EP++QRIADGD +P+LT  PI+T+SLSSGTT+G+ K+VPF     ++T+
Sbjct: 74  NCVPIVTHNDLEPYIQRIADGDLSPILTGKPIETISLSSGTTQGKPKFVPFNDELMESTM 133

Query: 141 LIFRLAAAYRSRVYPIREGGKIVEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQLKT 200
            IF+ + A+R+R +PI   GK ++FIYSSKQ KTKGG+  GTATT+ Y + +FK      
Sbjct: 134 QIFKTSFAFRNREFPI-GNGKALQFIYSSKQFKTKGGLAAGTATTNVYRNAQFKKTMKAM 193

Query: 201 QSFTCSPEEVISGGDYKQSTYCHLLLGLFFAHQVEFVTSTFAYTILQAFNQLEEVWPDIC 260
            +  CSP+EVI G D+ QS YCHLL GL F  +V+ V+STFA++I+ AF   E+VW  + 
Sbjct: 194 CTPCCSPDEVIFGPDFHQSLYCHLLCGLIFHDEVQVVSSTFAHSIVHAFRTFEQVWEALV 253

Query: 261 RDITHASLSSTVTIPKMRRAVLDIISPNPSLASSIRASCQRLRARDWFGLIPELWPNAKY 320
            DI    LSS VT+P +R A+  ++ P+P LA +I   C RL   +W+GLIP+L+PN +Y
Sbjct: 254 VDIREGVLSSRVTVPSIRLAMSKLLKPDPELADTIYNKCSRL--SNWYGLIPDLFPNTRY 313

Query: 321 VYSITTGSMQPYVKKLRHYAAGLPLVSADYGSTESWIGVNVDPSLPPENVTFAVVPTFSY 380
           +Y I TGSM+PY+KKLRHYA  LPL+SADYGS+E W+GVNV+P LPPE VT+AV+P   Y
Sbjct: 314 IYGIMTGSMEPYLKKLRHYAGELPLLSADYGSSEGWVGVNVNPKLPPELVTYAVLPNIGY 373

Query: 381 FEFIPLFRQHQNQNQICSSDFDIGVDDFLEGEPVPLSRVKIGQQYEVVLTTFTGLYRCRL 440
           FEFIPL     N N I  ++            PV L+ VK+G++YEVV T F GLYR RL
Sbjct: 374 FEFIPL---GGNLNGIEQAN-----------SPVGLTEVKLGEEYEVVFTNFAGLYRYRL 433

Query: 441 GDIVEVSGFHNKTPKLSFICRRKLVLTINIDKNTEKDLQMVVERGAQLLGDKSQSGAEVV 500
           GD+V+V GFHN TP+L F+CR  L+L+INIDKNTEKDLQ+ VE  A+ L D+     EVV
Sbjct: 434 GDVVKVKGFHNGTPELQFVCRSNLLLSINIDKNTEKDLQLAVEAAAKRLVDEK---LEVV 493

Query: 501 DFTSHADVADQPGHYVIFWEVKGDVEDAVLGECCTLMDAAFVDYGYVVSRRSNSIGPLEL 560
           DFTSH +V+  PGHYVIFWE+ G+  D +L +CC  +D +F+D GYV SR+ N+IG LEL
Sbjct: 494 DFTSHVNVSADPGHYVIFWELSGEATDEMLQDCCNCLDRSFIDAGYVSSRKVNAIGALEL 553

Query: 561 RVVERGTFKKILEHCIGNGGALSQFKTPRCT--TNQLLLSILNLSTVKTFRSTAY 614
           R+V+RGTF KIL+H +G GGA+SQFKTPRC    N  LL IL+ + V+T+ STA+
Sbjct: 554 RIVKRGTFHKILDHFVGLGGAVSQFKTPRCVGPKNSSLLQILSSNVVETYVSTAF 579

BLAST of MS004829 vs. ExPASy Swiss-Prot
Match: Q6I581 (Jasmonoyl--L-amino acid synthetase GH3.5 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 1.9e-166
Identity = 296/595 (49.75%), Postives = 400/595 (67.23%), Query Frame = 0

Query: 22  QEIIDWFEQVTSDAGVAQSRTLRQILQQNCGVEYLKPWLGDVNIHQIQDDDSALESLFTS 81
           +E I+ FE +T DA   Q  TL++IL+ N   EYL+    +  +    D +S     + S
Sbjct: 7   EETINEFEMLTRDAARVQKDTLKKILEINASAEYLQ----NFGLGGRTDAES-----YKS 66

Query: 82  SVPLSSHPHFEPFLQRIADGDSAPLLTQLPIKTLSLSSGTTEGRQKYVPFTPHSAQTTLL 141
            +PL  H   EP++QRI DGD++P++T  PI  LSLSSGTT G+ K++PF     +TTL 
Sbjct: 67  CIPLCVHNDIEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKPKFIPFNDELLETTLQ 126

Query: 142 IFRLAAAYRSRVYPIREGGKIVEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQLKTQ 201
           I+R + A+R+R YPI + GK ++F+Y SKQ  TKGGI   TATT+ Y  + +K      Q
Sbjct: 127 IYRTSYAFRNREYPIGQ-GKALQFVYGSKQVITKGGILATTATTNLYRRQRYKEGMKDIQ 186

Query: 202 SFTCSPEEVISGGDYKQSTYCHLLLGLFFAHQVEFVTSTFAYTILQAFNQLEEVWPDICR 261
           S  CSP+EVI G D+ QS YCHLL GL ++ +V  V STFA++++ AF   EEVW D+C 
Sbjct: 187 SQCCSPDEVIFGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAHSLVHAFQTFEEVWEDLCT 246

Query: 262 DITHASLSSTVTIPKMRRAVLDIISPNPSLASSIRASCQRLRARDWFGLIPELWPNAKYV 321
           DI    LS  VT P +R AV  I+ PNP LA SI   C  +   +W+G+IP LWPNAKYV
Sbjct: 247 DIRDGVLSKKVTAPSIREAVSKILKPNPELADSIYKKC--IGLSNWYGVIPALWPNAKYV 306

Query: 322 YSITTGSMQPYVKKLRHYAAGLPLVSADYGSTESWIGVNVDPSLPPENVTFAVVPTFSYF 381
           Y I TGSM+PY+KKLRHYA  LPL+SADYG++E W+G N+DP++PPE VT+AV+P   YF
Sbjct: 307 YGIMTGSMEPYLKKLRHYAGNLPLISADYGASEGWVGSNIDPTVPPEQVTYAVLPQVGYF 366

Query: 382 EFIPLFRQHQNQNQICSSDFDIGVDDFLEGEPVPLSRVKIGQQYEVVLTTFTGLYRCRLG 441
           EFIPL +    + +  +S        ++E +PV L+ V++G+ YEVV+T F GLYR RLG
Sbjct: 367 EFIPLEKPIGEETENSAS------IHYIESDPVGLTEVEVGKIYEVVITNFAGLYRYRLG 426

Query: 442 DIVEVSGFHNKTPKLSFICRRKLVLTINIDKNTEKDLQMVVERGAQLL-GDKSQSGAEVV 501
           D+V+++ FHN TP+L FICRR LVL+INIDKNTEKDLQ+ VE  ++ L G+K     EV+
Sbjct: 427 DVVKIARFHNSTPELQFICRRSLVLSINIDKNTEKDLQLAVEEASKFLEGEK----LEVM 486

Query: 502 DFTSHADVADQPGHYVIFWEVKGDVEDAVLGECCTLMDAAFVDYGYVVSRRSNSIGPLEL 561
           DFTS  + +  PG YVIFWE+ GD  D VL  C   +D AF+D GY  SR+  +IGPLEL
Sbjct: 487 DFTSFVERSSDPGRYVIFWELSGDASDEVLSSCANALDLAFIDAGYTGSRKIKTIGPLEL 546

Query: 562 RVVERGTFKKILEHCIGNGGALSQFKTPRCT--TNQLLLSILNLSTVKTFRSTAY 614
           R++ +GTFK+IL+H +  GGA+SQFKTPR    +N  +L IL+ +  +++ STAY
Sbjct: 547 RILRKGTFKEILDHFLSLGGAVSQFKTPRFVNPSNSKVLQILSRNVTQSYFSTAY 579

BLAST of MS004829 vs. ExPASy Swiss-Prot
Match: Q9SKE2 (Jasmonoyl--L-amino acid synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 PE=1 SV=2)

HSP 1 Score: 561.6 bits (1446), Expect = 1.1e-158
Identity = 280/592 (47.30%), Postives = 389/592 (65.71%), Query Frame = 0

Query: 24  IIDWFEQVTSDAGVAQSRTLRQILQQNCGVEYLKPWLGDVNIHQIQDDDSALESLFTSSV 83
           +ID F+++T +A   Q +TL++IL +N    YL+          +  + +  E  F S V
Sbjct: 13  VIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNC-------GLNGNATDPEEAFKSMV 72

Query: 84  PLSSHPHFEPFLQRIADGDSAPLLTQLPIKTLSLSSGTTEGRQKYVPFTPHSAQTTLLIF 143
           PL +    EP+++R+ DGD++P+LT  P+  +SLSSGT++GR K++PFT    + TL +F
Sbjct: 73  PLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQLF 132

Query: 144 RLAAAYRSRVYPIREGGKIVEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQLKTQSF 203
           R A A+R+R +PI + GK ++FI+SSKQ  + GG+  GTATT+ Y +  FK       S 
Sbjct: 133 RTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSITSP 192

Query: 204 TCSPEEVISGGDYKQSTYCHLLLGLFFAHQVEFVTSTFAYTILQAFNQLEEVWPDICRDI 263
           +CSP+EVI   D  Q+ YCHLL G+ F  QV++V + FA+ ++ AF   E+VW +I  DI
Sbjct: 193 SCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVTDI 252

Query: 264 THASLSSTVTIPKMRRAVLDIISPNPSLASSIRASCQRLRARDWFGLIPELWPNAKYVYS 323
               LS+ +T+P +R A+  +++PNP LA +IR  C  L   +W+GLIP L+PNAKYVY 
Sbjct: 253 KDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIPALFPNAKYVYG 312

Query: 324 ITTGSMQPYVKKLRHYAAGLPLVSADYGSTESWIGVNVDPSLPPENVTFAVVPTFSYFEF 383
           I TGSM+PYV KLRHYA  LPLVS DYGS+E WI  NV P L PE  TFAV+P   YFEF
Sbjct: 313 IMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEF 372

Query: 384 IPLFRQHQNQNQICSSDFDIGVDDFLEGEPVPLSRVKIGQQYEVVLTTFTGLYRCRLGDI 443
           +P+    +                  E +PV L++VKIG++YEVV+T + GLYR RLGD+
Sbjct: 373 LPVSETGEG-----------------EEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDV 432

Query: 444 VEVSGFHNKTPKLSFICRRKLVLTINIDKNTEKDLQMVVERGAQLLGDKSQSGAEVVDFT 503
           V+V GF+N TP+L FICRR L+L+INIDKNTE+DLQ+ VE  A+ L   S+   EV+DF+
Sbjct: 433 VKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRL---SEEKIEVIDFS 492

Query: 504 SHADVADQPGHYVIFWEVKGDVEDAVLGECCTLMDAAFVDYGYVVSRRSNSIGPLELRVV 563
           S+ DV+  PGHY IFWE+ G+  + VL +CC  +D AF+D GYV SR+  +IG LELRVV
Sbjct: 493 SYIDVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVV 552

Query: 564 ERGTFKKILEHCIGNGGALSQFKTPRCT--TNQLLLSILNLSTVKTFRSTAY 614
            +GTF+KI EH +G G +  QFK PRC   +N  +L IL  + V ++ STA+
Sbjct: 553 AKGTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

BLAST of MS004829 vs. ExPASy TrEMBL
Match: A0A6J1CHT7 (indole-3-acetic acid-amido synthetase GH3.10 OS=Momordica charantia OX=3673 GN=LOC111011043 PE=3 SV=1)

HSP 1 Score: 1234.6 bits (3193), Expect = 0.0e+00
Identity = 611/614 (99.51%), Postives = 612/614 (99.67%), Query Frame = 0

Query: 1   MEAEAEAGGAGNDHGELSEKEQEIIDWFEQVTSDAGVAQSRTLRQILQQNCGVEYLKPWL 60
           MEAEAEAGGAGNDHGELSEKEQEIIDWFEQVTSDAGVAQSRTLRQILQQNCGVEYLKPWL
Sbjct: 1   MEAEAEAGGAGNDHGELSEKEQEIIDWFEQVTSDAGVAQSRTLRQILQQNCGVEYLKPWL 60

Query: 61  GDVNIHQIQDDDSALESLFTSSVPLSSHPHFEPFLQRIADGDSAPLLTQLPIKTLSLSSG 120
           GDVNIHQIQDDDSALESLFTSSVPLSSHPHFEPFLQRIADGDS PLLTQLPIKTLSLSSG
Sbjct: 61  GDVNIHQIQDDDSALESLFTSSVPLSSHPHFEPFLQRIADGDSTPLLTQLPIKTLSLSSG 120

Query: 121 TTEGRQKYVPFTPHSAQTTLLIFRLAAAYRSRVYPIREGGKIVEFIYSSKQTKTKGGITT 180
           TTEGRQKYV FTPHSAQTTLLIFRLAAAYRSRVYPIREGGKIVEFIYSSKQTKTKGGITT
Sbjct: 121 TTEGRQKYVLFTPHSAQTTLLIFRLAAAYRSRVYPIREGGKIVEFIYSSKQTKTKGGITT 180

Query: 181 GTATTHYYASEEFKIKQLKTQSFTCSPEEVISGGDYKQSTYCHLLLGLFFAHQVEFVTST 240
           GTATTHYYASEEFKIKQLKTQSFTCSPEEVISGGDYKQSTYCHLLLGLFFAHQVEFVTST
Sbjct: 181 GTATTHYYASEEFKIKQLKTQSFTCSPEEVISGGDYKQSTYCHLLLGLFFAHQVEFVTST 240

Query: 241 FAYTILQAFNQLEEVWPDICRDITHASLSSTVTIPKMRRAVLDIISPNPSLASSIRASCQ 300
           FAYTILQAFNQLEEVWPDICRDITHASLSSTVTIPKMRRAVLDIISPNPSLASSIRASCQ
Sbjct: 241 FAYTILQAFNQLEEVWPDICRDITHASLSSTVTIPKMRRAVLDIISPNPSLASSIRASCQ 300

Query: 301 RLRARDWFGLIPELWPNAKYVYSITTGSMQPYVKKLRHYAAGLPLVSADYGSTESWIGVN 360
           RLRARDWFGLIPELWPNAKYVYSITTGSMQPYVKKLRHYAAGLPLVSADYGSTESWIGVN
Sbjct: 301 RLRARDWFGLIPELWPNAKYVYSITTGSMQPYVKKLRHYAAGLPLVSADYGSTESWIGVN 360

Query: 361 VDPSLPPENVTFAVVPTFSYFEFIPLFRQHQNQNQICSSDFDIGVDDFLEGEPVPLSRVK 420
           VDPSLPPENVTFAVVPTFSYFEFIPLFRQHQNQNQICSSDFDIGVDDFLEGEPVPLSRVK
Sbjct: 361 VDPSLPPENVTFAVVPTFSYFEFIPLFRQHQNQNQICSSDFDIGVDDFLEGEPVPLSRVK 420

Query: 421 IGQQYEVVLTTFTGLYRCRLGDIVEVSGFHNKTPKLSFICRRKLVLTINIDKNTEKDLQM 480
           IGQQYEVVLTTFTGLYRCRLGDIVEVSGFHNKTPKLSFICRRKLVLTINIDKNTEKDLQM
Sbjct: 421 IGQQYEVVLTTFTGLYRCRLGDIVEVSGFHNKTPKLSFICRRKLVLTINIDKNTEKDLQM 480

Query: 481 VVERGAQLLGDKSQSGAEVVDFTSHADVADQPGHYVIFWEVKGDVEDAVLGECCTLMDAA 540
           VVERGAQLLGDKSQSGAEVVDFTSHADVADQPGHYVIFWEVKGDVEDAVLGECCTLMDAA
Sbjct: 481 VVERGAQLLGDKSQSGAEVVDFTSHADVADQPGHYVIFWEVKGDVEDAVLGECCTLMDAA 540

Query: 541 FVDYGYVVSRRSNSIGPLELRVVERGTFKKILEHCIGNGGALSQFKTPRCTTNQLLLSIL 600
           FVD+GYVVSRRSNSIGPLELRVVERGTFKKILEHCIGNGGALSQFKTPRCTTNQLLLSIL
Sbjct: 541 FVDHGYVVSRRSNSIGPLELRVVERGTFKKILEHCIGNGGALSQFKTPRCTTNQLLLSIL 600

Query: 601 NLSTVKTFRSTAYA 615
           NLSTVKTFRSTAYA
Sbjct: 601 NLSTVKTFRSTAYA 614

BLAST of MS004829 vs. ExPASy TrEMBL
Match: A0A6J1J741 (indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita maxima OX=3661 GN=LOC111484069 PE=3 SV=1)

HSP 1 Score: 996.9 bits (2576), Expect = 3.7e-287
Identity = 480/595 (80.67%), Postives = 540/595 (90.76%), Query Frame = 0

Query: 19  EKEQEIIDWFEQVTSDAGVAQSRTLRQILQQNCGVEYLKPWLGDVNIHQIQDDDSALESL 78
           E+ ++++DWFE+V+S+AGVAQSRTLR+IL+QNCGVEYL  WLGDVNIHQI  DD ALESL
Sbjct: 3   EEVEDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQI-PDDFALESL 62

Query: 79  FTSSVPLSSHPHFEPFLQRIADGDSAPLLTQLPIKTLSLSSGTTEGRQKYVPFTPHSAQT 138
           FTS VPLSSH  FEPFLQRIADGDS+PLLTQ PI+TLSLSSGTTEGRQKYVPFTPHSAQT
Sbjct: 63  FTSLVPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQT 122

Query: 139 TLLIFRLAAAYRSRVYPIREGGKIVEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQL 198
           TLLIFR+AAAYRSRVYP REGG+I+EFIYSSKQTKTKGGITTGTATTHYYASE+FKIKQL
Sbjct: 123 TLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQL 182

Query: 199 KTQSFTCSPEEVISGGDYKQSTYCHLLLGLFFAHQVEFVTSTFAYTILQAFNQLEEVWPD 258
           KT+SFTCSP+EVISGGDYKQSTYCHLLLGLFF+ QVEFVTSTFAYTI+QAFN+LE+ WP 
Sbjct: 183 KTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQ 242

Query: 259 ICRDITHASLSSTVTIPKMRRAVLDIISPNPSLASSIRASCQRLRARDWFGLIPELWPNA 318
           IC DITHA+L S +T+P++R+AVL+IISPNP L + I+A C+ L  +DW  L+P+LW N 
Sbjct: 243 ICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSNC 302

Query: 319 KYVYSITTGSMQPYVKKLRHYAAGLPLVSADYGSTESWIGVNVDPSLPPENVTFAVVPTF 378
           KYVYSI TGSMQPY+KKLR YA GLPLVS DYGSTESWIG+NVDP LPPENVTFAVVPTF
Sbjct: 303 KYVYSIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPTF 362

Query: 379 SYFEFIPLFRQHQNQNQICSSDFDIGVDDFLEGEPVPLSRVKIGQQYEVVLTTFTGLYRC 438
           SYFEFIPLFRQHQ  +  C S      DDFLEG+P+PLS+VKIGQQYE+VLTTFTGLYRC
Sbjct: 363 SYFEFIPLFRQHQQHD--CGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRC 422

Query: 439 RLGDIVEVSGFHNKTPKLSFICRRKLVLTINIDKNTEKDLQMVVERGAQLLGDKSQSGAE 498
           RLGD+VEV+GFHNKTPKL+FICRRKLVLT+NIDKNTEKDLQ+VVERG+QLL D  QSGAE
Sbjct: 423 RLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLAD--QSGAE 482

Query: 499 VVDFTSHADVADQPGHYVIFWEVKGDVEDAVLGECCTLMDAAFVDYGYVVSRRSNSIGPL 558
           +VDFTSHAD+ +QPGHYVIFWEVKG+V+DAVLG CC+ MDAAFVDYGYVVSRRSN+IGPL
Sbjct: 483 LVDFTSHADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPL 542

Query: 559 ELRVVERGTFKKILEHCIGNGGALSQFKTPRCTTNQLLLSILNLSTVKTFRSTAY 614
           ELR+VERG+F KI+EH IGNG ALSQFKTPRCTTNQ+LL+ILNLSTVK F STAY
Sbjct: 543 ELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 592

BLAST of MS004829 vs. ExPASy TrEMBL
Match: A0A6J1FRQ1 (indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita moschata OX=3662 GN=LOC111448207 PE=3 SV=1)

HSP 1 Score: 993.4 bits (2567), Expect = 4.1e-286
Identity = 480/595 (80.67%), Postives = 536/595 (90.08%), Query Frame = 0

Query: 19  EKEQEIIDWFEQVTSDAGVAQSRTLRQILQQNCGVEYLKPWLGDVNIHQIQDDDSALESL 78
           E  ++++DWFE+V+S+AGVAQ RTLR+IL+QNCGVEYL  WLGDVNIHQI  DD ALESL
Sbjct: 3   EDVEDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQI-PDDFALESL 62

Query: 79  FTSSVPLSSHPHFEPFLQRIADGDSAPLLTQLPIKTLSLSSGTTEGRQKYVPFTPHSAQT 138
           FTS +PLSSH  FEPFLQRIADGDS+PLLTQ PI+TLSLSSGTTEGRQKYVPFTPHSAQT
Sbjct: 63  FTSLIPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQT 122

Query: 139 TLLIFRLAAAYRSRVYPIREGGKIVEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQL 198
           TLLIFR+AAAYRSRVYP REGG+I+EFIYSSKQTKTKGGITTGTATTHYYASE+FKIKQL
Sbjct: 123 TLLIFRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQL 182

Query: 199 KTQSFTCSPEEVISGGDYKQSTYCHLLLGLFFAHQVEFVTSTFAYTILQAFNQLEEVWPD 258
           KT+SFTCSP+EVISGGDYKQSTYCHLLLGLFF+ QVEFVTSTFAYTI+QAFN+LE+ WP 
Sbjct: 183 KTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQ 242

Query: 259 ICRDITHASLSSTVTIPKMRRAVLDIISPNPSLASSIRASCQRLRARDWFGLIPELWPNA 318
           IC DITHA+L S +T+P++R+AVL+IISPNP L + I A C+ L  +DW  L+P+LWPN 
Sbjct: 243 ICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNC 302

Query: 319 KYVYSITTGSMQPYVKKLRHYAAGLPLVSADYGSTESWIGVNVDPSLPPENVTFAVVPTF 378
           KYVYSI TGSMQPY+KKLR YA  LPLVS DYGSTESWIGVNVDP LPPENVTFAVVPTF
Sbjct: 303 KYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTF 362

Query: 379 SYFEFIPLFRQHQNQNQICSSDFDIGVDDFLEGEPVPLSRVKIGQQYEVVLTTFTGLYRC 438
           SYFEFIPLFRQHQ  +  C S      DDFLEG+P+PLS+VKIGQQYE+VLTTFTGLYRC
Sbjct: 363 SYFEFIPLFRQHQQHD--CGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRC 422

Query: 439 RLGDIVEVSGFHNKTPKLSFICRRKLVLTINIDKNTEKDLQMVVERGAQLLGDKSQSGAE 498
           RLGD+VEV+GFHNKTPKL+FICRRKLVLT+NIDKNTEKDLQ+VVERG+QLL D  QSGAE
Sbjct: 423 RLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLAD--QSGAE 482

Query: 499 VVDFTSHADVADQPGHYVIFWEVKGDVEDAVLGECCTLMDAAFVDYGYVVSRRSNSIGPL 558
           +VDFTSHAD+ +QPGHYVIFWEVKG VEDAVLG CC+ MDAAFVDYGYVVSRRSN+IGPL
Sbjct: 483 LVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPL 542

Query: 559 ELRVVERGTFKKILEHCIGNGGALSQFKTPRCTTNQLLLSILNLSTVKTFRSTAY 614
           ELR+VERG+F KI+EH IGNG ALSQFKTPRCTTNQ+LL+ILNLSTVK F STAY
Sbjct: 543 ELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 592

BLAST of MS004829 vs. ExPASy TrEMBL
Match: A0A5D3B9T4 (Jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold464G00300 PE=3 SV=1)

HSP 1 Score: 958.7 bits (2477), Expect = 1.1e-275
Identity = 464/599 (77.46%), Postives = 528/599 (88.15%), Query Frame = 0

Query: 16  ELSEKEQEIIDWFEQVTSDAGVAQSRTLRQILQQNCGVEYLKPWLGDVNIHQIQDDDSAL 75
           EL E+E++IIDWFEQV+S+A VAQSRTLR+ILQQN GVEYLK W+GDVNIH+I  DD  L
Sbjct: 5   ELCEEEEDIIDWFEQVSSEAAVAQSRTLRKILQQNYGVEYLKKWVGDVNIHEI-PDDFTL 64

Query: 76  ESLFTSSVPLSSHPHFEPFLQRIADGDSAPLLTQLPIKTLSLSSGTTEGRQKYVPFTPHS 135
           +S+FTS +PLSSH HFEPFLQRIADGDS+PLLTQ PI TLSLSSGTTEGRQKYVPFTPHS
Sbjct: 65  QSIFTSLIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFTPHS 124

Query: 136 AQTTLLIFRLAAAYRSRVYPIREGGKIVEFIYSSKQTKTKGGITTGTATTHYYASEEFKI 195
           A TTLLIFRLAAAYRSRVYP REGGKI+EFIYSSKQTKTKGGITTGTATTHYYASEEFKI
Sbjct: 125 AHTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFKI 184

Query: 196 KQLKTQSFTCSPEEVISGGDYKQSTYCHLLLGLFFAHQVEFVTSTFAYTILQAFNQLEEV 255
           KQLKT+SFTCSP+EVISG DYKQSTYCHLLLGL ++ +VEFVTSTFAYTI+QAFNQLEE 
Sbjct: 185 KQLKTKSFTCSPQEVISGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYTIVQAFNQLEES 244

Query: 256 WPDICRDITHASLSSTVTIPKMRRAVLDIISPNPSLASSIRASCQRLRARDWFGLIPELW 315
           W +I  DI+HA+LSS + IP++R+AVL ++SP P L   IR  C+ L+   W GLIP+LW
Sbjct: 245 WEEIIHDISHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELKKEGWLGLIPKLW 304

Query: 316 PNAKYVYSITTGSMQPYVKKLRHYAAGLPLVSADYGSTESWIGVNVDPSLPPENVTFAVV 375
           PN KYVYSI TGSMQPY+KKLRHY  GLPLVS DYGSTESWIGVNVDP LPPENVTFAV+
Sbjct: 305 PNCKYVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVI 364

Query: 376 PTFSYFEFIPLFRQHQNQNQICSSDFDIGVDDFLEGEPVPLSRVKIGQQYEVVLTTFTGL 435
           PTFSYFEFIPLF+QH       +S  D  + DFLEG+P+ LS VKIGQQYE+VLTTFTGL
Sbjct: 365 PTFSYFEFIPLFKQHAT-----TSSTDTAIHDFLEGQPIALSEVKIGQQYELVLTTFTGL 424

Query: 436 YRCRLGDIVEVSGFHNKTPKLSFICRRKLVLTINIDKNTEKDLQMVVERGAQLLGDKSQS 495
           YRCRLGD+VEV+GFHNKTPKL+FICRRKLVLT+NIDKNTEKD+Q+ VERG+QL+     S
Sbjct: 425 YRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGSQLM--SQLS 484

Query: 496 GAEVVDFTSHADVADQPGHYVIFWEVKGDVEDAVLGECCTLMDAAFVDYGYVVSRRSNSI 555
           GAE+VDFTS+A++++QPGHYVIFWEVKGDV D VLG+CC+ MDAAFVDYGYVVSR++NSI
Sbjct: 485 GAELVDFTSYAELSNQPGHYVIFWEVKGDVNDTVLGKCCSEMDAAFVDYGYVVSRKANSI 544

Query: 556 GPLELRVVERGTFKKILEHCIGNGGALSQFKTPRCTTNQLLLSILNLSTVKTFRSTAYA 615
           GPLELR+VERG+F KILE+ IGNG ALSQFKTPRCTTN  LL+ILNLST+++F STAYA
Sbjct: 545 GPLELRIVERGSFNKILEYYIGNGAALSQFKTPRCTTNHFLLNILNLSTLQSFFSTAYA 595

BLAST of MS004829 vs. ExPASy TrEMBL
Match: A0A1S3BIL6 (jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490483 PE=3 SV=1)

HSP 1 Score: 958.7 bits (2477), Expect = 1.1e-275
Identity = 464/599 (77.46%), Postives = 528/599 (88.15%), Query Frame = 0

Query: 16  ELSEKEQEIIDWFEQVTSDAGVAQSRTLRQILQQNCGVEYLKPWLGDVNIHQIQDDDSAL 75
           EL E+E++IIDWFEQV+S+A VAQSRTLR+ILQQN GVEYLK W+GDVNIH+I  DD  L
Sbjct: 5   ELCEEEEDIIDWFEQVSSEAAVAQSRTLRKILQQNYGVEYLKKWVGDVNIHEI-PDDFTL 64

Query: 76  ESLFTSSVPLSSHPHFEPFLQRIADGDSAPLLTQLPIKTLSLSSGTTEGRQKYVPFTPHS 135
           +S+FTS +PLSSH HFEPFLQRIADGDS+PLLTQ PI TLSLSSGTTEGRQKYVPFTPHS
Sbjct: 65  QSIFTSLIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFTPHS 124

Query: 136 AQTTLLIFRLAAAYRSRVYPIREGGKIVEFIYSSKQTKTKGGITTGTATTHYYASEEFKI 195
           A TTLLIFRLAAAYRSRVYP REGGKI+EFIYSSKQTKTKGGITTGTATTHYYASEEFKI
Sbjct: 125 AHTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFKI 184

Query: 196 KQLKTQSFTCSPEEVISGGDYKQSTYCHLLLGLFFAHQVEFVTSTFAYTILQAFNQLEEV 255
           KQLKT+SFTCSP+EVISG DYKQSTYCHLLLGL ++ +VEFVTSTFAYTI+QAFNQLEE 
Sbjct: 185 KQLKTKSFTCSPQEVISGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYTIVQAFNQLEES 244

Query: 256 WPDICRDITHASLSSTVTIPKMRRAVLDIISPNPSLASSIRASCQRLRARDWFGLIPELW 315
           W +I  DI+HA+LSS + IP++R+AVL ++SP P L   IR  C+ L+   W GLIP+LW
Sbjct: 245 WEEIIHDISHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELKKEGWLGLIPKLW 304

Query: 316 PNAKYVYSITTGSMQPYVKKLRHYAAGLPLVSADYGSTESWIGVNVDPSLPPENVTFAVV 375
           PN KYVYSI TGSMQPY+KKLRHY  GLPLVS DYGSTESWIGVNVDP LPPENVTFAV+
Sbjct: 305 PNCKYVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVI 364

Query: 376 PTFSYFEFIPLFRQHQNQNQICSSDFDIGVDDFLEGEPVPLSRVKIGQQYEVVLTTFTGL 435
           PTFSYFEFIPLF+QH       +S  D  + DFLEG+P+ LS VKIGQQYE+VLTTFTGL
Sbjct: 365 PTFSYFEFIPLFKQHAT-----TSSTDTAIHDFLEGQPIALSEVKIGQQYELVLTTFTGL 424

Query: 436 YRCRLGDIVEVSGFHNKTPKLSFICRRKLVLTINIDKNTEKDLQMVVERGAQLLGDKSQS 495
           YRCRLGD+VEV+GFHNKTPKL+FICRRKLVLT+NIDKNTEKD+Q+ VERG+QL+     S
Sbjct: 425 YRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGSQLM--SQLS 484

Query: 496 GAEVVDFTSHADVADQPGHYVIFWEVKGDVEDAVLGECCTLMDAAFVDYGYVVSRRSNSI 555
           GAE+VDFTS+A++++QPGHYVIFWEVKGDV D VLG+CC+ MDAAFVDYGYVVSR++NSI
Sbjct: 485 GAELVDFTSYAELSNQPGHYVIFWEVKGDVNDTVLGKCCSEMDAAFVDYGYVVSRKANSI 544

Query: 556 GPLELRVVERGTFKKILEHCIGNGGALSQFKTPRCTTNQLLLSILNLSTVKTFRSTAYA 615
           GPLELR+VERG+F KILE+ IGNG ALSQFKTPRCTTN  LL+ILNLST+++F STAYA
Sbjct: 545 GPLELRIVERGSFNKILEYYIGNGAALSQFKTPRCTTNHFLLNILNLSTLQSFFSTAYA 595

BLAST of MS004829 vs. TAIR 10
Match: AT4G03400.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 823.5 bits (2126), Expect = 1.1e-238
Identity = 403/592 (68.07%), Postives = 480/592 (81.08%), Query Frame = 0

Query: 23  EIIDWFEQVTSDAGVAQSRTLRQILQQNCGVEYLKPWLGDVNIHQIQDDDSALESLFTSS 82
           ++I WFE V+ +A   QS TLR+IL+ N GVEYL+ WLG V++ ++  DD  LE+LFTS 
Sbjct: 10  DVIGWFEHVSENACKVQSETLRRILELNSGVEYLRKWLGTVDVEKM--DDYTLETLFTSL 69

Query: 83  VPLSSHPHFEPFLQRIADGDSAPLLTQLPIKTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 142
           VP+ SH   +P++QRIADG+++PLLTQ PI  LSLSSGTTEGRQKYVPFT HSAQTTL I
Sbjct: 70  VPIVSHADLDPYIQRIADGETSPLLTQEPITVLSLSSGTTEGRQKYVPFTRHSAQTTLQI 129

Query: 143 FRLAAAYRSRVYPIREGGKIVEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQLKTQS 202
           FRL+AAYRSR YPIREGG+I+EFIY+ K+ KT GG+T GTATTHYYASEEFK KQ  T+S
Sbjct: 130 FRLSAAYRSRFYPIREGGRILEFIYAGKEFKTLGGLTVGTATTHYYASEEFKTKQETTKS 189

Query: 203 FTCSPEEVISGGDYKQSTYCHLLLGLFFAHQVEFVTSTFAYTILQAFNQLEEVWPDICRD 262
           FTCSP+EVISGGD+ Q TYCHLLLGL ++ QVEFV S F+YTI+QAF+  EE+W +IC D
Sbjct: 190 FTCSPQEVISGGDFGQCTYCHLLLGLHYSSQVEFVASAFSYTIVQAFSFFEEIWREICAD 249

Query: 263 ITHASLSSTVTIPKMRRAVLDIISPNPSLASSIRASCQRLRAR-DWFGLIPELWPNAKYV 322
           I   +LSS +T+PKMR+AVL +I PNPSLAS I   C  L     WFGLI +LWPNAK++
Sbjct: 250 IKEGNLSSRITLPKMRKAVLALIRPNPSLASHIEEICLELETNLGWFGLISKLWPNAKFI 309

Query: 323 YSITTGSMQPYVKKLRHYAAGLPLVSADYGSTESWIGVNVDPSLPPENVTFAVVPTFSYF 382
            SI TGSM PY+ KLRHYA GLPLVSADYGSTESWIGVNVDP LPPE+V+FAV+PTFSYF
Sbjct: 310 SSIMTGSMLPYLNKLRHYAGGLPLVSADYGSTESWIGVNVDPHLPPEDVSFAVIPTFSYF 369

Query: 383 EFIPLFRQHQNQNQICSSDFDIGVDDFLEGEPVPLSRVKIGQQYEVVLTTFTGLYRCRLG 442
           EFIPL+R+ QNQ+ IC         DF+E +PVPLS+VK+GQ+YE+VLTTFTGLYR RLG
Sbjct: 370 EFIPLYRR-QNQSDICID------GDFVEDKPVPLSQVKLGQEYELVLTTFTGLYRYRLG 429

Query: 443 DIVEVSGFHNKTPKLSFICRRKLVLTINIDKNTEKDLQMVVERGAQLLGDKSQSGAEVVD 502
           D+VEV+ FH  TPKLSFI RRKL+LTINIDKNTEKDLQ VV++ +QLL   ++  AEVVD
Sbjct: 430 DVVEVTSFHKGTPKLSFIYRRKLILTINIDKNTEKDLQRVVDKASQLLSRSTR--AEVVD 489

Query: 503 FTSHADVADQPGHYVIFWEVKGDVEDAVLGECCTLMDAAFVDYGYVVSRRSNSIGPLELR 562
           FTSHADV  +PGHYVI+WE++G+ +D  L ECC  MD AFVDYGYVVSRR NSIGPLELR
Sbjct: 490 FTSHADVIARPGHYVIYWEIRGEADDKALEECCREMDTAFVDYGYVVSRRMNSIGPLELR 549

Query: 563 VVERGTFKKILEHCIGNGGALSQFKTPRCTTNQLLLSILNLSTVKTFRSTAY 614
           VVERGTF K+ E C+G  G L+QFKTPRCTTN ++L ILN ST+K FRS+AY
Sbjct: 550 VVERGTFGKVAERCVGKCGGLNQFKTPRCTTNSVMLDILNDSTIKRFRSSAY 590

BLAST of MS004829 vs. TAIR 10
Match: AT2G46370.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 561.6 bits (1446), Expect = 7.7e-160
Identity = 280/592 (47.30%), Postives = 389/592 (65.71%), Query Frame = 0

Query: 24  IIDWFEQVTSDAGVAQSRTLRQILQQNCGVEYLKPWLGDVNIHQIQDDDSALESLFTSSV 83
           +ID F+++T +A   Q +TL++IL +N    YL+          +  + +  E  F S V
Sbjct: 13  VIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNC-------GLNGNATDPEEAFKSMV 72

Query: 84  PLSSHPHFEPFLQRIADGDSAPLLTQLPIKTLSLSSGTTEGRQKYVPFTPHSAQTTLLIF 143
           PL +    EP+++R+ DGD++P+LT  P+  +SLSSGT++GR K++PFT    + TL +F
Sbjct: 73  PLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQLF 132

Query: 144 RLAAAYRSRVYPIREGGKIVEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQLKTQSF 203
           R A A+R+R +PI + GK ++FI+SSKQ  + GG+  GTATT+ Y +  FK       S 
Sbjct: 133 RTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSITSP 192

Query: 204 TCSPEEVISGGDYKQSTYCHLLLGLFFAHQVEFVTSTFAYTILQAFNQLEEVWPDICRDI 263
           +CSP+EVI   D  Q+ YCHLL G+ F  QV++V + FA+ ++ AF   E+VW +I  DI
Sbjct: 193 SCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVTDI 252

Query: 264 THASLSSTVTIPKMRRAVLDIISPNPSLASSIRASCQRLRARDWFGLIPELWPNAKYVYS 323
               LS+ +T+P +R A+  +++PNP LA +IR  C  L   +W+GLIP L+PNAKYVY 
Sbjct: 253 KDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIPALFPNAKYVYG 312

Query: 324 ITTGSMQPYVKKLRHYAAGLPLVSADYGSTESWIGVNVDPSLPPENVTFAVVPTFSYFEF 383
           I TGSM+PYV KLRHYA  LPLVS DYGS+E WI  NV P L PE  TFAV+P   YFEF
Sbjct: 313 IMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEF 372

Query: 384 IPLFRQHQNQNQICSSDFDIGVDDFLEGEPVPLSRVKIGQQYEVVLTTFTGLYRCRLGDI 443
           +P+    +                  E +PV L++VKIG++YEVV+T + GLYR RLGD+
Sbjct: 373 LPVSETGEG-----------------EEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDV 432

Query: 444 VEVSGFHNKTPKLSFICRRKLVLTINIDKNTEKDLQMVVERGAQLLGDKSQSGAEVVDFT 503
           V+V GF+N TP+L FICRR L+L+INIDKNTE+DLQ+ VE  A+ L   S+   EV+DF+
Sbjct: 433 VKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRL---SEEKIEVIDFS 492

Query: 504 SHADVADQPGHYVIFWEVKGDVEDAVLGECCTLMDAAFVDYGYVVSRRSNSIGPLELRVV 563
           S+ DV+  PGHY IFWE+ G+  + VL +CC  +D AF+D GYV SR+  +IG LELRVV
Sbjct: 493 SYIDVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVV 552

Query: 564 ERGTFKKILEHCIGNGGALSQFKTPRCT--TNQLLLSILNLSTVKTFRSTAY 614
            +GTF+KI EH +G G +  QFK PRC   +N  +L IL  + V ++ STA+
Sbjct: 553 AKGTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

BLAST of MS004829 vs. TAIR 10
Match: AT2G46370.2 (Auxin-responsive GH3 family protein )

HSP 1 Score: 561.6 bits (1446), Expect = 7.7e-160
Identity = 280/592 (47.30%), Postives = 389/592 (65.71%), Query Frame = 0

Query: 24  IIDWFEQVTSDAGVAQSRTLRQILQQNCGVEYLKPWLGDVNIHQIQDDDSALESLFTSSV 83
           +ID F+++T +A   Q +TL++IL +N    YL+          +  + +  E  F S V
Sbjct: 13  VIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNC-------GLNGNATDPEEAFKSMV 72

Query: 84  PLSSHPHFEPFLQRIADGDSAPLLTQLPIKTLSLSSGTTEGRQKYVPFTPHSAQTTLLIF 143
           PL +    EP+++R+ DGD++P+LT  P+  +SLSSGT++GR K++PFT    + TL +F
Sbjct: 73  PLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQLF 132

Query: 144 RLAAAYRSRVYPIREGGKIVEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQLKTQSF 203
           R A A+R+R +PI + GK ++FI+SSKQ  + GG+  GTATT+ Y +  FK       S 
Sbjct: 133 RTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSITSP 192

Query: 204 TCSPEEVISGGDYKQSTYCHLLLGLFFAHQVEFVTSTFAYTILQAFNQLEEVWPDICRDI 263
           +CSP+EVI   D  Q+ YCHLL G+ F  QV++V + FA+ ++ AF   E+VW +I  DI
Sbjct: 193 SCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVTDI 252

Query: 264 THASLSSTVTIPKMRRAVLDIISPNPSLASSIRASCQRLRARDWFGLIPELWPNAKYVYS 323
               LS+ +T+P +R A+  +++PNP LA +IR  C  L   +W+GLIP L+PNAKYVY 
Sbjct: 253 KDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIPALFPNAKYVYG 312

Query: 324 ITTGSMQPYVKKLRHYAAGLPLVSADYGSTESWIGVNVDPSLPPENVTFAVVPTFSYFEF 383
           I TGSM+PYV KLRHYA  LPLVS DYGS+E WI  NV P L PE  TFAV+P   YFEF
Sbjct: 313 IMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEF 372

Query: 384 IPLFRQHQNQNQICSSDFDIGVDDFLEGEPVPLSRVKIGQQYEVVLTTFTGLYRCRLGDI 443
           +P+    +                  E +PV L++VKIG++YEVV+T + GLYR RLGD+
Sbjct: 373 LPVSETGEG-----------------EEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDV 432

Query: 444 VEVSGFHNKTPKLSFICRRKLVLTINIDKNTEKDLQMVVERGAQLLGDKSQSGAEVVDFT 503
           V+V GF+N TP+L FICRR L+L+INIDKNTE+DLQ+ VE  A+ L   S+   EV+DF+
Sbjct: 433 VKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRL---SEEKIEVIDFS 492

Query: 504 SHADVADQPGHYVIFWEVKGDVEDAVLGECCTLMDAAFVDYGYVVSRRSNSIGPLELRVV 563
           S+ DV+  PGHY IFWE+ G+  + VL +CC  +D AF+D GYV SR+  +IG LELRVV
Sbjct: 493 SYIDVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVV 552

Query: 564 ERGTFKKILEHCIGNGGALSQFKTPRCT--TNQLLLSILNLSTVKTFRSTAY 614
            +GTF+KI EH +G G +  QFK PRC   +N  +L IL  + V ++ STA+
Sbjct: 553 AKGTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

BLAST of MS004829 vs. TAIR 10
Match: AT2G46370.4 (Auxin-responsive GH3 family protein )

HSP 1 Score: 561.6 bits (1446), Expect = 7.7e-160
Identity = 280/592 (47.30%), Postives = 389/592 (65.71%), Query Frame = 0

Query: 24  IIDWFEQVTSDAGVAQSRTLRQILQQNCGVEYLKPWLGDVNIHQIQDDDSALESLFTSSV 83
           +ID F+++T +A   Q +TL++IL +N    YL+          +  + +  E  F S V
Sbjct: 24  VIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNC-------GLNGNATDPEEAFKSMV 83

Query: 84  PLSSHPHFEPFLQRIADGDSAPLLTQLPIKTLSLSSGTTEGRQKYVPFTPHSAQTTLLIF 143
           PL +    EP+++R+ DGD++P+LT  P+  +SLSSGT++GR K++PFT    + TL +F
Sbjct: 84  PLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQLF 143

Query: 144 RLAAAYRSRVYPIREGGKIVEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQLKTQSF 203
           R A A+R+R +PI + GK ++FI+SSKQ  + GG+  GTATT+ Y +  FK       S 
Sbjct: 144 RTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSITSP 203

Query: 204 TCSPEEVISGGDYKQSTYCHLLLGLFFAHQVEFVTSTFAYTILQAFNQLEEVWPDICRDI 263
           +CSP+EVI   D  Q+ YCHLL G+ F  QV++V + FA+ ++ AF   E+VW +I  DI
Sbjct: 204 SCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVTDI 263

Query: 264 THASLSSTVTIPKMRRAVLDIISPNPSLASSIRASCQRLRARDWFGLIPELWPNAKYVYS 323
               LS+ +T+P +R A+  +++PNP LA +IR  C  L   +W+GLIP L+PNAKYVY 
Sbjct: 264 KDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIPALFPNAKYVYG 323

Query: 324 ITTGSMQPYVKKLRHYAAGLPLVSADYGSTESWIGVNVDPSLPPENVTFAVVPTFSYFEF 383
           I TGSM+PYV KLRHYA  LPLVS DYGS+E WI  NV P L PE  TFAV+P   YFEF
Sbjct: 324 IMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEF 383

Query: 384 IPLFRQHQNQNQICSSDFDIGVDDFLEGEPVPLSRVKIGQQYEVVLTTFTGLYRCRLGDI 443
           +P+    +                  E +PV L++VKIG++YEVV+T + GLYR RLGD+
Sbjct: 384 LPVSETGEG-----------------EEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDV 443

Query: 444 VEVSGFHNKTPKLSFICRRKLVLTINIDKNTEKDLQMVVERGAQLLGDKSQSGAEVVDFT 503
           V+V GF+N TP+L FICRR L+L+INIDKNTE+DLQ+ VE  A+ L   S+   EV+DF+
Sbjct: 444 VKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRL---SEEKIEVIDFS 503

Query: 504 SHADVADQPGHYVIFWEVKGDVEDAVLGECCTLMDAAFVDYGYVVSRRSNSIGPLELRVV 563
           S+ DV+  PGHY IFWE+ G+  + VL +CC  +D AF+D GYV SR+  +IG LELRVV
Sbjct: 504 SYIDVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVV 563

Query: 564 ERGTFKKILEHCIGNGGALSQFKTPRCT--TNQLLLSILNLSTVKTFRSTAY 614
            +GTF+KI EH +G G +  QFK PRC   +N  +L IL  + V ++ STA+
Sbjct: 564 AKGTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 586

BLAST of MS004829 vs. TAIR 10
Match: AT2G46370.3 (Auxin-responsive GH3 family protein )

HSP 1 Score: 528.1 bits (1359), Expect = 9.5e-150
Identity = 258/518 (49.81%), Postives = 350/518 (67.57%), Query Frame = 0

Query: 98  IADGDSAPLLTQLPIKTLSLSSGTTEGRQKYVPFTPHSAQTTLLIFRLAAAYRSRVYPIR 157
           + DGD++P+LT  P+  +SLSSGT++GR K++PFT    + TL +FR A A+R+R +PI 
Sbjct: 1   MVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQLFRTAFAFRNRDFPID 60

Query: 158 EGGKIVEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQLKTQSFTCSPEEVISGGDYK 217
           + GK ++FI+SSKQ  + GG+  GTATT+ Y +  FK       S +CSP+EVI   D  
Sbjct: 61  DNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSITSPSCSPDEVIFSPDVH 120

Query: 218 QSTYCHLLLGLFFAHQVEFVTSTFAYTILQAFNQLEEVWPDICRDITHASLSSTVTIPKM 277
           Q+ YCHLL G+ F  QV++V + FA+ ++ AF   E+VW +I  DI    LS+ +T+P +
Sbjct: 121 QALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVTDIKDGVLSNRITVPSV 180

Query: 278 RRAVLDIISPNPSLASSIRASCQRLRARDWFGLIPELWPNAKYVYSITTGSMQPYVKKLR 337
           R A+  +++PNP LA +IR  C  L   +W+GLIP L+PNAKYVY I TGSM+PYV KLR
Sbjct: 181 RTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIPALFPNAKYVYGIMTGSMEPYVPKLR 240

Query: 338 HYAAGLPLVSADYGSTESWIGVNVDPSLPPENVTFAVVPTFSYFEFIPLFRQHQNQNQIC 397
           HYA  LPLVS DYGS+E WI  NV P L PE  TFAV+P   YFEF+P+    +      
Sbjct: 241 HYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEFLPVSETGEG----- 300

Query: 398 SSDFDIGVDDFLEGEPVPLSRVKIGQQYEVVLTTFTGLYRCRLGDIVEVSGFHNKTPKLS 457
                       E +PV L++VKIG++YEVV+T + GLYR RLGD+V+V GF+N TP+L 
Sbjct: 301 ------------EEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKVIGFYNNTPQLK 360

Query: 458 FICRRKLVLTINIDKNTEKDLQMVVERGAQLLGDKSQSGAEVVDFTSHADVADQPGHYVI 517
           FICRR L+L+INIDKNTE+DLQ+ VE  A+ L   S+   EV+DF+S+ DV+  PGHY I
Sbjct: 361 FICRRNLILSINIDKNTERDLQLSVESAAKRL---SEEKIEVIDFSSYIDVSTDPGHYAI 420

Query: 518 FWEVKGDVEDAVLGECCTLMDAAFVDYGYVVSRRSNSIGPLELRVVERGTFKKILEHCIG 577
           FWE+ G+  + VL +CC  +D AF+D GYV SR+  +IG LELRVV +GTF+KI EH +G
Sbjct: 421 FWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTFRKIQEHFLG 480

Query: 578 NGGALSQFKTPRCT--TNQLLLSILNLSTVKTFRSTAY 614
            G +  QFK PRC   +N  +L IL  + V ++ STA+
Sbjct: 481 LGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 496