MS004827 (gene) Bitter gourd (TR) v1

Overview
NameMS004827
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionProtein phosphatase 2C family protein
Locationscaffold176: 854977 .. 856804 (+)
RNA-Seq ExpressionMS004827
SyntenyMS004827
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGAGCTGTTTGTCTACTGGCTATCAGAGTAGTTGCCATGGTGGAAGCAATGGAGAGACAGTTTCTGGGTCCTGCTGTTTGGGAATCGGAGTTGGTGGTCGGATAGCAAGAAAGAGAACATTCTCAGATCATATCTTTTCTCTGCAGCATTTGAATTCTATGCCAAATAGACTTGTGTTTAATGGAAAGACTCGAAATTCTTGCATTTTCACGCAACAGGGTCGCAAGGGTATAAACCAGGATGCCATGGTTGTGTGGGAAGTAAGTTCTTCTACTTATTCTCGTCGGATTGTTTGGTTCAATTCAGTTTAAACAATGTACAAAAGGGTTAATTAATAAAAAATTTCCCGTGTTGTGTTTTTTTACTTGTTTCCTGATGAGGAGTTCAATTTCTCTAGGACTTTATATCCGATGGTGCAGTCTTTTGTGGGATATTTGATGGTCACGGTCCTCACGGTCATGTTGTTGCACGAAAAGTTAGAGACGTTCTACCAAGAAAGCTAGTGTCATTCTGGCAGTCATCTCAGTTGAAGCATAATGAATCAGATAGAAGTTCTTGTTTCGGGTGGGGGCGGGGGAATTCCGCAGATGGTGAAGGGTTGGAGACGGCTGGATCAGAGGAGGATAAATCAAGTTCTTTATGGAGAAGAGCTTTCCTAAAATCATATAAATCCATGGATAAGGAGCTCAAATCTCATCCCAAATTGGACTGTTTCTGTAGTGGTAGTACTGCAGTCACTCTAGTGAAGCAGGTACATATAATAAACGCTGATAAATGAGCAAGTTTTGCTACATTCTAAAACGGTTTTATTTATTTTCCTCCAGGGCTCAGATCTATTCGTTGGAAGTATCGGGGATTCTCGAGCAATTCTTGGATCTAAAGACAGTTGTGATTCCCTTGTAGCTACCCAATTGACAGTAGATCTAAAGCCTGATTTGCCCAGTATGTCGAATCCATTGCTTACTCTAATGAACTGATATGGATTCTAATTTGCTCGGGTTGTTTGCCGTGTAGGAGAAGCCGAAAGGATAAAGAAGTGTAAAGGGAGGGTATTTGCATTGCAAGATGAGCCAGAAGTGCCAAGAGTGTGGCTGCCATTTGACAATGCGCCAGGATTAGCCATGGCTCGAGCATTTGGCGACTTCTGCTTGAAGGACTATGGAGTTATCTCTATTCCCGAGTTCTCGCATCGCTTGCTTACGGATTATGACCTGTTTATTGTGCTTGCTTCAGATGGGGTAACTTTTCTAGTCCACCCACTTCACTTGTAAAACTAAAGAACATTGTCTATATAGCTGGCAAGCAGAAGCAAGCATGTGTTTGAAGTTTTATTTCGATTGTCAATCTCCAGAATTCAAGACAGTTCCCTTGCAGGTTTGGGACGTATTGAGCAATGAAGAGGTAGTCGAGATAGTGAATTCCACCTCGACTCGGTCATCTGCAGCTCGAATGGTGGTAGACGCGGCTGCTCGTGAGTGGAAGCTCAAGTACCCAACTTCGAAAATGGATGACTGTGCAGTAATTTGCCTATTCTTGGATGGGAAAATGGATTCAGAATCAGAGAATGAAGAACAAGCTTCTTCTGCTGCTCTTCAAACCAATCAAATGGGCAACAACACTGCAGATTCAGATGAAGAGCAGAGGTCTGAGCCAAGCTTGCAAAGGAACAACACGGTCCGATCATCGGAAGAAAACGACCAAATTGGAGGACTATCTTCACATGGTGAAGAGAATGGAGAAACAATGTCTGCTGAAGATGAGAATTGGTTGGGATTGGAGGGTGTAACAAGAGTAAACTCTCTAGTTCAACTCCCTAGATTTTCT

mRNA sequence

ATGGGGAGCTGTTTGTCTACTGGCTATCAGAGTAGTTGCCATGGTGGAAGCAATGGAGAGACAGTTTCTGGGTCCTGCTGTTTGGGAATCGGAGTTGGTGGTCGGATAGCAAGAAAGAGAACATTCTCAGATCATATCTTTTCTCTGCAGCATTTGAATTCTATGCCAAATAGACTTGTGTTTAATGGAAAGACTCGAAATTCTTGCATTTTCACGCAACAGGGTCGCAAGGGTATAAACCAGGATGCCATGGTTGTGTGGGAAGACTTTATATCCGATGGTGCAGTCTTTTGTGGGATATTTGATGGTCACGGTCCTCACGGTCATGTTGTTGCACGAAAAGTTAGAGACGTTCTACCAAGAAAGCTAGTGTCATTCTGGCAGTCATCTCAGTTGAAGCATAATGAATCAGATAGAAGTTCTTGTTTCGGGTGGGGGCGGGGGAATTCCGCAGATGGTGAAGGGTTGGAGACGGCTGGATCAGAGGAGGATAAATCAAGTTCTTTATGGAGAAGAGCTTTCCTAAAATCATATAAATCCATGGATAAGGAGCTCAAATCTCATCCCAAATTGGACTGTTTCTGTAGTGGTAGTACTGCAGTCACTCTAGTGAAGCAGGGCTCAGATCTATTCGTTGGAAGTATCGGGGATTCTCGAGCAATTCTTGGATCTAAAGACAGTTGTGATTCCCTTGTAGCTACCCAATTGACAGTAGATCTAAAGCCTGATTTGCCCAGAGAAGCCGAAAGGATAAAGAAGTGTAAAGGGAGGGTATTTGCATTGCAAGATGAGCCAGAAGTGCCAAGAGTGTGGCTGCCATTTGACAATGCGCCAGGATTAGCCATGGCTCGAGCATTTGGCGACTTCTGCTTGAAGGACTATGGAGTTATCTCTATTCCCGAGTTCTCGCATCGCTTGCTTACGGATTATGACCTGTTTATTGTGCTTGCTTCAGATGGGGTTTGGGACGTATTGAGCAATGAAGAGGTAGTCGAGATAGTGAATTCCACCTCGACTCGGTCATCTGCAGCTCGAATGGTGGTAGACGCGGCTGCTCGTGAGTGGAAGCTCAAGTACCCAACTTCGAAAATGGATGACTGTGCAGTAATTTGCCTATTCTTGGATGGGAAAATGGATTCAGAATCAGAGAATGAAGAACAAGCTTCTTCTGCTGCTCTTCAAACCAATCAAATGGGCAACAACACTGCAGATTCAGATGAAGAGCAGAGGTCTGAGCCAAGCTTGCAAAGGAACAACACGGTCCGATCATCGGAAGAAAACGACCAAATTGGAGGACTATCTTCACATGGTGAAGAGAATGGAGAAACAATGTCTGCTGAAGATGAGAATTGGTTGGGATTGGAGGGTGTAACAAGAGTAAACTCTCTAGTTCAACTCCCTAGATTTTCT

Coding sequence (CDS)

ATGGGGAGCTGTTTGTCTACTGGCTATCAGAGTAGTTGCCATGGTGGAAGCAATGGAGAGACAGTTTCTGGGTCCTGCTGTTTGGGAATCGGAGTTGGTGGTCGGATAGCAAGAAAGAGAACATTCTCAGATCATATCTTTTCTCTGCAGCATTTGAATTCTATGCCAAATAGACTTGTGTTTAATGGAAAGACTCGAAATTCTTGCATTTTCACGCAACAGGGTCGCAAGGGTATAAACCAGGATGCCATGGTTGTGTGGGAAGACTTTATATCCGATGGTGCAGTCTTTTGTGGGATATTTGATGGTCACGGTCCTCACGGTCATGTTGTTGCACGAAAAGTTAGAGACGTTCTACCAAGAAAGCTAGTGTCATTCTGGCAGTCATCTCAGTTGAAGCATAATGAATCAGATAGAAGTTCTTGTTTCGGGTGGGGGCGGGGGAATTCCGCAGATGGTGAAGGGTTGGAGACGGCTGGATCAGAGGAGGATAAATCAAGTTCTTTATGGAGAAGAGCTTTCCTAAAATCATATAAATCCATGGATAAGGAGCTCAAATCTCATCCCAAATTGGACTGTTTCTGTAGTGGTAGTACTGCAGTCACTCTAGTGAAGCAGGGCTCAGATCTATTCGTTGGAAGTATCGGGGATTCTCGAGCAATTCTTGGATCTAAAGACAGTTGTGATTCCCTTGTAGCTACCCAATTGACAGTAGATCTAAAGCCTGATTTGCCCAGAGAAGCCGAAAGGATAAAGAAGTGTAAAGGGAGGGTATTTGCATTGCAAGATGAGCCAGAAGTGCCAAGAGTGTGGCTGCCATTTGACAATGCGCCAGGATTAGCCATGGCTCGAGCATTTGGCGACTTCTGCTTGAAGGACTATGGAGTTATCTCTATTCCCGAGTTCTCGCATCGCTTGCTTACGGATTATGACCTGTTTATTGTGCTTGCTTCAGATGGGGTTTGGGACGTATTGAGCAATGAAGAGGTAGTCGAGATAGTGAATTCCACCTCGACTCGGTCATCTGCAGCTCGAATGGTGGTAGACGCGGCTGCTCGTGAGTGGAAGCTCAAGTACCCAACTTCGAAAATGGATGACTGTGCAGTAATTTGCCTATTCTTGGATGGGAAAATGGATTCAGAATCAGAGAATGAAGAACAAGCTTCTTCTGCTGCTCTTCAAACCAATCAAATGGGCAACAACACTGCAGATTCAGATGAAGAGCAGAGGTCTGAGCCAAGCTTGCAAAGGAACAACACGGTCCGATCATCGGAAGAAAACGACCAAATTGGAGGACTATCTTCACATGGTGAAGAGAATGGAGAAACAATGTCTGCTGAAGATGAGAATTGGTTGGGATTGGAGGGTGTAACAAGAGTAAACTCTCTAGTTCAACTCCCTAGATTTTCT

Protein sequence

MGSCLSTGYQSSCHGGSNGETVSGSCCLGIGVGGRIARKRTFSDHIFSLQHLNSMPNRLVFNGKTRNSCIFTQQGRKGINQDAMVVWEDFISDGAVFCGIFDGHGPHGHVVARKVRDVLPRKLVSFWQSSQLKHNESDRSSCFGWGRGNSADGEGLETAGSEEDKSSSLWRRAFLKSYKSMDKELKSHPKLDCFCSGSTAVTLVKQGSDLFVGSIGDSRAILGSKDSCDSLVATQLTVDLKPDLPREAERIKKCKGRVFALQDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIPEFSHRLLTDYDLFIVLASDGVWDVLSNEEVVEIVNSTSTRSSAARMVVDAAAREWKLKYPTSKMDDCAVICLFLDGKMDSESENEEQASSAALQTNQMGNNTADSDEEQRSEPSLQRNNTVRSSEENDQIGGLSSHGEENGETMSAEDENWLGLEGVTRVNSLVQLPRFS
Homology
BLAST of MS004827 vs. NCBI nr
Match: XP_022140736.1 (probable protein phosphatase 2C 52 [Momordica charantia])

HSP 1 Score: 932.2 bits (2408), Expect = 1.8e-267
Identity = 467/470 (99.36%), Postives = 468/470 (99.57%), Query Frame = 0

Query: 1   MGSCLSTGYQSSCHGGSNGETVSGSCCLGIGVGGRIARKRTFSDHIFSLQHLNSMPNRLV 60
           MGSCLSTGYQSSCHGGSNGETVSGSCCLGIGVGGRIARK TFSDHIFSLQHLNS+PNRLV
Sbjct: 1   MGSCLSTGYQSSCHGGSNGETVSGSCCLGIGVGGRIARKITFSDHIFSLQHLNSIPNRLV 60

Query: 61  FNGKTRNSCIFTQQGRKGINQDAMVVWEDFISDGAVFCGIFDGHGPHGHVVARKVRDVLP 120
           FNGKTRNSCIFTQQGRKGINQDAMVVWEDFISDGAVFCGIFDGHGPHGHVVARKVRDVLP
Sbjct: 61  FNGKTRNSCIFTQQGRKGINQDAMVVWEDFISDGAVFCGIFDGHGPHGHVVARKVRDVLP 120

Query: 121 RKLVSFWQSSQLKHNESDRSSCFGWGRGNSADGEGLETAGSEEDKSSSLWRRAFLKSYKS 180
           RKLVSFWQSSQLKHN SDRSSCFGWGRGNSADGEGLETAGSEEDKSSSLWRRAFLKSYKS
Sbjct: 121 RKLVSFWQSSQLKHNGSDRSSCFGWGRGNSADGEGLETAGSEEDKSSSLWRRAFLKSYKS 180

Query: 181 MDKELKSHPKLDCFCSGSTAVTLVKQGSDLFVGSIGDSRAILGSKDSCDSLVATQLTVDL 240
           MDKELKSHPKLDCFCSGSTAVTLVKQGSDLFVGSIGDSRAILGSKDSCDSLVATQLTVDL
Sbjct: 181 MDKELKSHPKLDCFCSGSTAVTLVKQGSDLFVGSIGDSRAILGSKDSCDSLVATQLTVDL 240

Query: 241 KPDLPREAERIKKCKGRVFALQDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP 300
           KPDLPREAERIKKCKGRVFALQDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP
Sbjct: 241 KPDLPREAERIKKCKGRVFALQDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP 300

Query: 301 EFSHRLLTDYDLFIVLASDGVWDVLSNEEVVEIVNSTSTRSSAARMVVDAAAREWKLKYP 360
           EFSHRLLTDYDLFIVLASDGVWDVLSNEEVVEIVNSTSTRSSAARMVVDAAAREWKLKYP
Sbjct: 301 EFSHRLLTDYDLFIVLASDGVWDVLSNEEVVEIVNSTSTRSSAARMVVDAAAREWKLKYP 360

Query: 361 TSKMDDCAVICLFLDGKMDSESENEEQASSAALQTNQMGNNTADSDEEQRSEPSLQRNNT 420
           TSKMDDCAVICLFLDGKMDSESENEEQASSAALQTNQMGNNTADSDEEQRSEPSLQRNNT
Sbjct: 361 TSKMDDCAVICLFLDGKMDSESENEEQASSAALQTNQMGNNTADSDEEQRSEPSLQRNNT 420

Query: 421 VRSSEENDQIGGLSSHGEENGETMSAEDENWLGLEGVTRVNSLVQLPRFS 471
           VRSSEENDQIGGLSSHGEENGETMSAEDENWLGLEGVTRVNSLVQLPRFS
Sbjct: 421 VRSSEENDQIGGLSSHGEENGETMSAEDENWLGLEGVTRVNSLVQLPRFS 470

BLAST of MS004827 vs. NCBI nr
Match: XP_038902772.1 (probable protein phosphatase 2C 52 [Benincasa hispida])

HSP 1 Score: 816.2 bits (2107), Expect = 1.4e-232
Identity = 421/471 (89.38%), Postives = 438/471 (92.99%), Query Frame = 0

Query: 1   MGSCLSTGYQSSCHGGSNGETVSGSCCLGIGVGGRIARKRTFSDHIFSLQHLNSMPNRLV 60
           MGSCLSTG Q SC GG NGE VS S CLGIGV GRI RKRTFSDHIFSLQ LNS PNRLV
Sbjct: 1   MGSCLSTGNQDSCKGGRNGERVSRS-CLGIGVCGRIERKRTFSDHIFSLQLLNSAPNRLV 60

Query: 61  FNGKTRNSCIFTQQGRKGINQDAMVVWEDFISDGAVFCGIFDGHGPHGHVVARKVRDVLP 120
           +NGKTRNSCIFTQQGRKGINQDAMVVWEDFISDGA+FCG+FDGHGPHGHVVARKVRDVLP
Sbjct: 61  YNGKTRNSCIFTQQGRKGINQDAMVVWEDFISDGAIFCGVFDGHGPHGHVVARKVRDVLP 120

Query: 121 RKLVSFWQSSQLKHNESDRSSCFGWGRGNSADGEGLETAGSEEDKSSSLWRRAFLKSYKS 180
           RKLVSFWQSSQLKHN SDR+SC    R +SADGEG +  GS+EDKSSSLWRRAFLKSYK+
Sbjct: 121 RKLVSFWQSSQLKHNRSDRTSCLP-SRKDSADGEGQDIDGSQEDKSSSLWRRAFLKSYKA 180

Query: 181 MDKELKSHPKLDCFCSGSTAVTLVKQGSDLFVGSIGDSRAILGSKDSCDSLVATQLTVDL 240
           MDKELKSHPKLDCFCSGSTAVTLVKQGSDLFVGSIGDSRAILGSKDS DSLVATQLTVDL
Sbjct: 181 MDKELKSHPKLDCFCSGSTAVTLVKQGSDLFVGSIGDSRAILGSKDSSDSLVATQLTVDL 240

Query: 241 KPDLPREAERIKKCKGRVFALQDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP 300
           KPDLPREAERIKKCKGRVFAL DEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP
Sbjct: 241 KPDLPREAERIKKCKGRVFALHDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP 300

Query: 301 EFSHRLLTDYDLFIVLASDGVWDVLSNEEVVEIVNSTSTRSSAARMVVDAAAREWKLKYP 360
           EFSHR+LT+ DLF+VLASDGVWDVLSNEEVVEIV S STRS AARMVVD+AAREWKLKYP
Sbjct: 301 EFSHRVLTNDDLFVVLASDGVWDVLSNEEVVEIVYSASTRSLAARMVVDSAAREWKLKYP 360

Query: 361 TSKMDDCAVICLFLDGKMDSESENEEQASSAALQTNQMGNNTADSDEEQRSEPSLQRNNT 420
           TSKMDDCAV+CLFL GKMDSESENEEQASSA LQTNQ+G NTADSD++QRSEPSLQRN T
Sbjct: 361 TSKMDDCAVVCLFLAGKMDSESENEEQASSATLQTNQIG-NTADSDDDQRSEPSLQRNYT 420

Query: 421 VRSSEENDQIGGL-SSHGEENGETMSAEDENWLGLEGVTRVNSLVQLPRFS 471
           VRSSEE DQIGGL SSH EENGE+MSAEDENWLGLEGVTRVNSLVQLPRFS
Sbjct: 421 VRSSEEQDQIGGLSSSHNEENGESMSAEDENWLGLEGVTRVNSLVQLPRFS 468

BLAST of MS004827 vs. NCBI nr
Match: KAG7010695.1 (putative protein phosphatase 2C 52 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 804.7 bits (2077), Expect = 4.3e-229
Identity = 409/471 (86.84%), Postives = 433/471 (91.93%), Query Frame = 0

Query: 1   MGSCLSTGYQSSCHGGSNGETVSGSCCLGIGVGGRIARKRTFSDHIFSLQHLNSMPNRLV 60
           MGSCLS+G QSSC+GGSNGE V  S CLG GV GRI R RTFSDH+FSLQHLNS+PNRLV
Sbjct: 1   MGSCLSSGNQSSCNGGSNGEGVFES-CLGNGVCGRIERNRTFSDHVFSLQHLNSVPNRLV 60

Query: 61  FNGKTRNSCIFTQQGRKGINQDAMVVWEDFISDGAVFCGIFDGHGPHGHVVARKVRDVLP 120
           +NGKT NSCIFTQQGRKGINQDAMVVWEDFISDGA+FCG+FDGHGPHGHVVARKVRDVLP
Sbjct: 61  YNGKTSNSCIFTQQGRKGINQDAMVVWEDFISDGAIFCGVFDGHGPHGHVVARKVRDVLP 120

Query: 121 RKLVSFWQSSQLKHNESDRSSCFGWGRGNSADGEGLETAGSEEDKSSSLWRRAFLKSYKS 180
           RKLVSFWQSSQLKH+ SDR+SC  W R NSADGEG +  GS+ED+SSSLWRR FLKSYK+
Sbjct: 121 RKLVSFWQSSQLKHDRSDRTSCLAWSRKNSADGEGQDVDGSQEDRSSSLWRRTFLKSYKA 180

Query: 181 MDKELKSHPKLDCFCSGSTAVTLVKQGSDLFVGSIGDSRAILGSKDSCDSLVATQLTVDL 240
           MDKELKS PKLDCFCSG TA+TLVKQGSDLFVGSIGDSRAILGSKDS DSLVATQLTVDL
Sbjct: 181 MDKELKSDPKLDCFCSGCTAITLVKQGSDLFVGSIGDSRAILGSKDSSDSLVATQLTVDL 240

Query: 241 KPDLPREAERIKKCKGRVFALQDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP 300
           KPDLPREAERIKKCKGRVFAL DEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP
Sbjct: 241 KPDLPREAERIKKCKGRVFALHDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP 300

Query: 301 EFSHRLLTDYDLFIVLASDGVWDVLSNEEVVEIVNSTSTRSSAARMVVDAAAREWKLKYP 360
           EFSHR LTD DLF+VLASDGVWDVLSN+EVVEIV S STRSSAARMVVD+AAREWKLKYP
Sbjct: 301 EFSHRALTDNDLFVVLASDGVWDVLSNDEVVEIVYSASTRSSAARMVVDSAAREWKLKYP 360

Query: 361 TSKMDDCAVICLFLDGKMDSESENEEQASSAALQTNQMGNNTADSDEEQRS-EPSLQRNN 420
           TSKMDDCAV+CLFLDGKMDSESENEEQAS A L++NQM +NT  SDEEQRS EPSLQRN 
Sbjct: 361 TSKMDDCAVVCLFLDGKMDSESENEEQASCATLESNQM-SNTECSDEEQRSGEPSLQRNY 420

Query: 421 TVRSSEENDQIGGLSSHGEENGETMSAEDENWLGLEGVTRVNSLVQLPRFS 471
           TVRSSEE D+IGGL SH EEN E+MSAEDENWLGL+GVTRVNSLVQLPRFS
Sbjct: 421 TVRSSEEKDRIGGLYSHSEENEESMSAEDENWLGLDGVTRVNSLVQLPRFS 469

BLAST of MS004827 vs. NCBI nr
Match: XP_022944671.1 (probable protein phosphatase 2C 52 [Cucurbita moschata])

HSP 1 Score: 800.4 bits (2066), Expect = 8.1e-228
Identity = 408/471 (86.62%), Postives = 432/471 (91.72%), Query Frame = 0

Query: 1   MGSCLSTGYQSSCHGGSNGETVSGSCCLGIGVGGRIARKRTFSDHIFSLQHLNSMPNRLV 60
           MGSCLS+G QSSC+GGSNGE V  S CLG GV GRI R RTFSDH+FSLQHLNS+PNRLV
Sbjct: 1   MGSCLSSGNQSSCNGGSNGEGVFES-CLGNGVCGRIERNRTFSDHVFSLQHLNSVPNRLV 60

Query: 61  FNGKTRNSCIFTQQGRKGINQDAMVVWEDFISDGAVFCGIFDGHGPHGHVVARKVRDVLP 120
           +NGKT NSCIFTQQGRKGINQDAMVVWEDFISDGA+FCG+FDGHGPHGHVVARKVRDVLP
Sbjct: 61  YNGKTSNSCIFTQQGRKGINQDAMVVWEDFISDGAIFCGVFDGHGPHGHVVARKVRDVLP 120

Query: 121 RKLVSFWQSSQLKHNESDRSSCFGWGRGNSADGEGLETAGSEEDKSSSLWRRAFLKSYKS 180
           RKLVSFWQSSQLK + SDR+SC  W R NSADGEG +  GS+ED+SSSLWRR FLKSYK+
Sbjct: 121 RKLVSFWQSSQLKRDRSDRTSCLAWSRKNSADGEGQDIDGSQEDRSSSLWRRTFLKSYKA 180

Query: 181 MDKELKSHPKLDCFCSGSTAVTLVKQGSDLFVGSIGDSRAILGSKDSCDSLVATQLTVDL 240
           MDKELKS PKLDCFCSG TA+TLVKQGSDLFVGSIGDSRAILGSKDS DSLVATQLTVDL
Sbjct: 181 MDKELKSDPKLDCFCSGCTAITLVKQGSDLFVGSIGDSRAILGSKDSSDSLVATQLTVDL 240

Query: 241 KPDLPREAERIKKCKGRVFALQDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP 300
           KPDLPREAERIKKCKGRVFAL DEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP
Sbjct: 241 KPDLPREAERIKKCKGRVFALHDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP 300

Query: 301 EFSHRLLTDYDLFIVLASDGVWDVLSNEEVVEIVNSTSTRSSAARMVVDAAAREWKLKYP 360
           EFSHR LTD DLF+VLASDGVWDVLSN+EVVEIV S STRSSAARMVVD+AAREWKLKYP
Sbjct: 301 EFSHRALTDNDLFVVLASDGVWDVLSNDEVVEIVYSASTRSSAARMVVDSAAREWKLKYP 360

Query: 361 TSKMDDCAVICLFLDGKMDSESENEEQASSAALQTNQMGNNTADSDEEQRS-EPSLQRNN 420
           TSKMDDCAV+CLFLDGKMDSESENEEQAS A L++NQM +NT  SDEEQRS EPSLQRN 
Sbjct: 361 TSKMDDCAVVCLFLDGKMDSESENEEQASCATLESNQM-SNTECSDEEQRSGEPSLQRNY 420

Query: 421 TVRSSEENDQIGGLSSHGEENGETMSAEDENWLGLEGVTRVNSLVQLPRFS 471
           TVRSSEE D+IGGL SH EEN E+MSAEDE+WLGLEGVTRVNSLVQLPRFS
Sbjct: 421 TVRSSEEKDRIGGLYSHSEENEESMSAEDESWLGLEGVTRVNSLVQLPRFS 469

BLAST of MS004827 vs. NCBI nr
Match: XP_023513362.1 (probable protein phosphatase 2C 52 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 797.3 bits (2058), Expect = 6.9e-227
Identity = 410/472 (86.86%), Postives = 430/472 (91.10%), Query Frame = 0

Query: 1   MGSCLSTGYQSSCHGGSNGETVSGSCCLGIGVGGRIARKRTFSDHIFSLQHLNSMPNRLV 60
           MGSCLS+G QSSC+GGSNGE V  S CLG GV GRI R RTFSDH+FSLQHLNS+PNRLV
Sbjct: 1   MGSCLSSGNQSSCNGGSNGEGVFES-CLGNGVCGRIERNRTFSDHVFSLQHLNSVPNRLV 60

Query: 61  FNGKTRNSCIFTQQGRKGINQDAMVVWEDFISDGAVFCGIFDGHGPHGHVVARKVRDVLP 120
           +NGKT NSCIFTQQGRKGINQDAMVVWEDFISDGA+FCG+FDGHGPHGHVVARKVRDVLP
Sbjct: 61  YNGKTSNSCIFTQQGRKGINQDAMVVWEDFISDGAIFCGLFDGHGPHGHVVARKVRDVLP 120

Query: 121 RKLVSFWQSSQLKHNESDRSSCFGWGRGNSADGEGLETAGSEEDKSSSLWRRAFLKSYKS 180
           RKLVSFWQSSQLK + SDR+SC  W R NSADGEG +  GS+ED+SSSLWRR FLKSYK+
Sbjct: 121 RKLVSFWQSSQLKRDRSDRTSCLAWRRKNSADGEGQDIDGSQEDRSSSLWRRTFLKSYKA 180

Query: 181 MDKELKSHPKLDCFCSGSTAVTLVKQGSDLFVGSIGDSRAILGSKDSCDSLVATQLTVDL 240
           MDKELKS PKLDCFCSG TA+ LVKQGSDLFVGSIGDSRAILGSKDS DSLVATQLTVDL
Sbjct: 181 MDKELKSDPKLDCFCSGCTAIALVKQGSDLFVGSIGDSRAILGSKDSSDSLVATQLTVDL 240

Query: 241 KPDLPREAERIKKCKGRVFALQDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP 300
           KPDLPREAERIKKCKGRVFAL DEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP
Sbjct: 241 KPDLPREAERIKKCKGRVFALHDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP 300

Query: 301 EFSHRLLTDYDLFIVLASDGVWDVLSNEEVVEIVNSTSTRSSAARMVVDAAAREWKLKYP 360
           EFSHR LTD DLF+VLASDGVWDVLSN+EVVEIV S STRSSAARMVVD AAREWKLKYP
Sbjct: 301 EFSHRALTDNDLFVVLASDGVWDVLSNDEVVEIVYSASTRSSAARMVVDLAAREWKLKYP 360

Query: 361 TSKMDDCAVICLFLDGKMDSESENEEQASSAALQTNQMGNNTADSDEEQRS-EPSLQRNN 420
           TSKMDDCAV+CLFLDGKMDSESENEEQAS A L+TNQM +NT  SDEEQRS EPSLQRN 
Sbjct: 361 TSKMDDCAVVCLFLDGKMDSESENEEQASCATLETNQM-SNTECSDEEQRSGEPSLQRNY 420

Query: 421 TVR-SSEENDQIGGLSSHGEENGETMSAEDENWLGLEGVTRVNSLVQLPRFS 471
           TVR SSEE DQIGGL SH EEN E+MSAEDENWLGLEGVTRVNSLVQLPRFS
Sbjct: 421 TVRSSSEEKDQIGGLHSHSEENEESMSAEDENWLGLEGVTRVNSLVQLPRFS 470

BLAST of MS004827 vs. ExPASy Swiss-Prot
Match: Q8GY60 (Probable protein phosphatase 2C 52 OS=Arabidopsis thaliana OX=3702 GN=At4g03415 PE=1 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 1.0e-172
Identity = 317/472 (67.16%), Postives = 379/472 (80.30%), Query Frame = 0

Query: 1   MGSCLSTGYQSSCHGGSNGETVSGSCCLGIGVGGRIARKRTFSDHIFSLQHLNSMPNRLV 60
           MG C+ST  +S+C   SNGE       LGIG       KRTFSDHI SLQ+L S+PNR+ 
Sbjct: 1   MGGCVSTSSKSTCSSWSNGEKPVRRPYLGIGCCVSKRAKRTFSDHIVSLQNLTSIPNRIT 60

Query: 61  FNGKTRNSCIFTQQGRKGINQDAMVVWEDFISDGAVFCGIFDGHGPHGHVVARKVRDVLP 120
            + K+R+SCIFTQQGRKGINQDAM+VWEDF+S+   FCG+FDGHGP+GH+VARKVRD LP
Sbjct: 61  SSSKSRSSCIFTQQGRKGINQDAMIVWEDFMSEDVTFCGVFDGHGPYGHLVARKVRDTLP 120

Query: 121 RKLVSFWQSSQLKHNESDRSSCFGWGRGNSADGEGLETAGSEEDKSSSLWRRAFLKSYKS 180
            KL  F+Q+ Q K N S + + F      SA  E ++  GS+EDK   LW  AFLKS+K+
Sbjct: 121 VKLQFFFQTLQSKQNCS-KGTRFRRNSSKSAVQEAVK-EGSDEDKLKGLWGEAFLKSFKA 180

Query: 181 MDKELKSHPKLDCFCSGSTAVTLVKQGSDLFVGSIGDSRAILGSKDSCDSLVATQLTVDL 240
           MDKEL+SHP LDCFCSGST VT++KQGS+LF+G+IGDSRAILGSKDS DS+VATQLTVDL
Sbjct: 181 MDKELRSHPNLDCFCSGSTGVTILKQGSNLFMGNIGDSRAILGSKDSNDSMVATQLTVDL 240

Query: 241 KPDLPREAERIKKCKGRVFALQDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP 300
           KPDLPREAERIK+CKGRVFA++DEPEVPRVWLP+D+APGLAMARAFGDFCLK+YGVIS+P
Sbjct: 241 KPDLPREAERIKRCKGRVFAMEDEPEVPRVWLPYDDAPGLAMARAFGDFCLKEYGVISVP 300

Query: 301 EFSHRLLTDYDLFIVLASDGVWDVLSNEEVVEIVNSTSTRSSAARMVVDAAAREWKLKYP 360
           EF+HR+LTD D FIVLASDGVWDVLSNEEVV+IV S ++R+SAAR +V++AAREWKLKYP
Sbjct: 301 EFTHRVLTDRDQFIVLASDGVWDVLSNEEVVDIVASATSRASAARTLVNSAAREWKLKYP 360

Query: 361 TSKMDDCAVICLFLDGKMDSESENEEQASSAALQTNQMGNNTADSDEEQRSEPSLQRNNT 420
           TSKMDDCAV+CLFLDGKMDSES+ +EQ  S+A        N  +SD+ QRSEP LQRN T
Sbjct: 361 TSKMDDCAVVCLFLDGKMDSESDYDEQGFSSA-------TNAVESDDGQRSEPCLQRNFT 420

Query: 421 VRSS--EENDQIGGLSSHGEENGETMSAEDENWLGLEGVTRVNSLVQLPRFS 471
           VRSS  +EN+  G +++  +   E  +  D+NWLGL+GVTRVNSLVQLPRFS
Sbjct: 421 VRSSSDQENETYGNVNTETDAEDE-KTVGDQNWLGLQGVTRVNSLVQLPRFS 462

BLAST of MS004827 vs. ExPASy Swiss-Prot
Match: Q9LR65 (Probable protein phosphatase 2C 1 OS=Arabidopsis thaliana OX=3702 GN=PPC6-6 PE=1 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 1.5e-163
Identity = 307/474 (64.77%), Postives = 361/474 (76.16%), Query Frame = 0

Query: 1   MGSCLSTGYQSSCHGGSNGETVSGSCCLGIGVGGRIARKRTFSDHIFSLQHLNSMPNRLV 60
           MG C+S    S+       E      CLG+G  G    KR FSD + SL +L S+PNR++
Sbjct: 1   MGGCISKTSWSN-------EEPMHRPCLGMGCCGSKMGKRGFSDRMVSLHNLVSIPNRII 60

Query: 61  FNGKTRNSCIFTQQGRKGINQDAMVVWEDFISDGAVFCGIFDGHGPHGHVVARKVRDVLP 120
            NGK+R+SCIFTQQGRKGINQDAM+VWEDF+S    FCG+FDGHGPHGH+VARKVRD LP
Sbjct: 61  GNGKSRSSCIFTQQGRKGINQDAMIVWEDFMSKDVTFCGVFDGHGPHGHLVARKVRDSLP 120

Query: 121 RKLVSFWQSSQLKHNESDRSSCFGWGRGNSADGEGLETAGSEEDKSSSLWRRAFLKSYKS 180
            KL+S   S + K N        G     S   E  +   +EEDK + LW  AFLKS+ +
Sbjct: 121 VKLLSLLNSIKSKQN-----GPIGTRASKSDSLEAEKEESTEEDKLNFLWEEAFLKSFNA 180

Query: 181 MDKELKSHPKLDCFCSGSTAVTLVKQGSDLFVGSIGDSRAILGSKDSCDSLVATQLTVDL 240
           MDKEL+SHP L+CFCSG TAVT++KQGS+L++G+IGDSRAILGSKDS DS++A QLTVDL
Sbjct: 181 MDKELRSHPNLECFCSGCTAVTIIKQGSNLYMGNIGDSRAILGSKDSNDSMIAVQLTVDL 240

Query: 241 KPDLPREAERIKKCKGRVFALQDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP 300
           KPDLPREAERIK+CKGRVFALQDEPEV RVWLPFDNAPGLAMARAFGDFCLKDYGVISIP
Sbjct: 241 KPDLPREAERIKQCKGRVFALQDEPEVSRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP 300

Query: 301 EFSHRLLTDYDLFIVLASDGVWDVLSNEEVVEIVNSTSTRSSAARMVVDAAAREWKLKYP 360
           EFSHR+LTD D FIVLASDGVWDVLSNEEVVE+V S ++R+SAAR+VVD+A REWKLKYP
Sbjct: 301 EFSHRVLTDRDQFIVLASDGVWDVLSNEEVVEVVASATSRASAARLVVDSAVREWKLKYP 360

Query: 361 TSKMDDCAVICLFLDGKMDSE-SENEEQASSAALQTNQMGNNTADSDEEQRSEPSLQRNN 420
           TSKMDDCAV+CLFLDG+MDSE S+NEEQ  S+A        N  +SDE Q +EP LQRN 
Sbjct: 361 TSKMDDCAVVCLFLDGRMDSETSDNEEQCFSSA-------TNAVESDESQGAEPCLQRNV 420

Query: 421 TVRS---SEENDQIGGLSSHGEENGETMSAE-DENWLGLEGVTRVNSLVQLPRF 470
           TVRS    +EN+  G + +  +   +  + E ++NW GLEGVTRVNSLVQLPRF
Sbjct: 421 TVRSLSTDQENNSYGKVIAEADNAEKEKTREGEQNWSGLEGVTRVNSLVQLPRF 455

BLAST of MS004827 vs. ExPASy Swiss-Prot
Match: Q8H4S6 (Probable protein phosphatase 2C 64 OS=Oryza sativa subsp. japonica OX=39947 GN=Os07g0566200 PE=2 SV=2)

HSP 1 Score: 460.3 bits (1183), Expect = 2.6e-128
Identity = 250/473 (52.85%), Postives = 322/473 (68.08%), Query Frame = 0

Query: 1   MGSCLSTGYQSSCHGGSNGETVSGSCCLGIGVGGRIARKRTFSDHIFSLQHLNSMPNRLV 60
           MG+C++    +   GG  GE             G+  R+R  +        L  +P R+ 
Sbjct: 1   MGNCVARSGTAVDAGGDGGE------------DGKRRRRRWKAPR---EDQLGMVPGRIF 60

Query: 61  FN-GKTRNSCIFTQQGRKGINQDAMVVWEDF-ISDGAVFCGIFDGHGPHGHVVARKVRDV 120
            N G++R + ++TQQGRKGINQDAM+VW+ F   D  V CG+FDGHGPHGHVVAR+VRD 
Sbjct: 61  SNDGRSRTATVYTQQGRKGINQDAMLVWDGFGGEDDGVLCGVFDGHGPHGHVVARRVRDS 120

Query: 121 LPRKLVSFWQSSQLKHNESDRSSCFGWGRGNSADGEGLETAGSEEDKSSSLWRRAFLKSY 180
           LP +L+S                                   S  D  ++ WR+AF ++Y
Sbjct: 121 LPLRLMS-------------------------------AARDSGADMPAAAWRKAFARAY 180

Query: 181 KSMDKELKSHPKLDCFCSGSTAVTLVKQGSDLFVGSIGDSRAILGSKDSC-DSLVATQLT 240
           K+MDK+L+SHP LDCFCSGSTAVT++K GSDL++ +IGDSRA+LGS+++    +VA QLT
Sbjct: 181 KAMDKDLRSHPSLDCFCSGSTAVTVLKLGSDLYMANIGDSRAVLGSREATGGGMVAVQLT 240

Query: 241 VDLKPDLPREAERIKKCKGRVFALQDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVI 300
           VDLKPD+P EAERIKKC+GRVFALQDEPEVPRVWLPFD+APGLAMARAFGDFCLKDYGVI
Sbjct: 241 VDLKPDVPSEAERIKKCRGRVFALQDEPEVPRVWLPFDDAPGLAMARAFGDFCLKDYGVI 300

Query: 301 SIPEFSHRLLTDYDLFIVLASDGVWDVLSNEEVVEIVNSTSTRSSAARMVVDAAAREWKL 360
           S+PEF H  LT+ D F++LASDGVWDVLSN+E V+IV+++ +RS AA+ +V+AA REWK 
Sbjct: 301 SVPEFFHWSLTEKDQFVILASDGVWDVLSNQEAVDIVSASPSRSKAAKSLVEAATREWKT 360

Query: 361 KYPTSKMDDCAVICLFLDGKMDSESENEEQASSAALQTNQMGNNTADSDEEQRSEPSLQR 420
           KYPTSK+DDCAV+CL+LDGKMD E ++     + +++      + AD +E Q  EP+L R
Sbjct: 361 KYPTSKIDDCAVVCLYLDGKMDHERDSTASLDNISIEE----GSVADPNEPQEQEPTLTR 420

Query: 421 NNTVRSSEENDQIGGLSSHGEENGETMSAEDENWLGLEGVTRVNSLVQLPRFS 471
           N TVR+   + Q   L+  G +      A D+NW GL+GVTRVNSLVQLPRFS
Sbjct: 421 NFTVRTVAGSTQEKTLA--GVDARIAGVANDQNWSGLDGVTRVNSLVQLPRFS 421

BLAST of MS004827 vs. ExPASy Swiss-Prot
Match: Q6ZKL8 (Probable protein phosphatase 2C 66 OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0500300 PE=2 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 4.2e-118
Identity = 240/484 (49.59%), Postives = 319/484 (65.91%), Query Frame = 0

Query: 15  GGSNGETVS--GSCCLGIGVGGRIARKRTFSDHIFSLQHLNSMPNRLVFNGKTRNSCIFT 74
           G   G  VS  G     IGVGG+            + + L  +  R+  NG +  +C+ T
Sbjct: 34  GVERGGAVSGGGGGVFSIGVGGKKLHHGGGGGGEMTEEELAKVEGRVCVNGASAAACLHT 93

Query: 75  QQGRKGINQDAMVVWEDFISDGAVFCGIFDGHGPHGHVVARKVRDVLPRKLVSFWQSSQL 134
           QQGRKG NQDAMVVWE+F +  +VFCG+FDGHGP+GH VA+KVRD LP K+ + W++S  
Sbjct: 94  QQGRKGTNQDAMVVWENFNTSDSVFCGVFDGHGPYGHFVAKKVRDSLPVKIRTLWKTSAN 153

Query: 135 KHNESDRSSCFGWGRGNSADGEGLETA-GSEEDKSSSL------WRRAFLKSYKSMDKEL 194
           +   S ++     G  NS +   ++   G   D S  L       ++++ K++K MDKEL
Sbjct: 154 EDTSSHQNGSIS-GSVNSEESPVVDDEWGEYADDSEKLPEMFLPLKQSYFKAFKLMDKEL 213

Query: 195 KSHPKLDCFCSGSTAVTLVKQGSDLFVGSIGDSRAILGSKDSCDSLVATQLTVDLKPDLP 254
           K HP +DCFCSGSTAVTLVKQG DL VG++GDSRAI+G++D+ ++L A QLTVDLKP+LP
Sbjct: 214 KMHPTVDCFCSGSTAVTLVKQGLDLVVGNLGDSRAIMGTRDAANNLTAVQLTVDLKPNLP 273

Query: 255 REAERIKKCKGRVFALQDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIPEFSHR 314
           REA RI++C+GRVFALQDEPEV RVWLP +++PGLAMARAFGDFCLKDYG+IS+P+ S+R
Sbjct: 274 REAARIQQCRGRVFALQDEPEVARVWLPNNDSPGLAMARAFGDFCLKDYGLISVPQISYR 333

Query: 315 LLTDYDLFIVLASDGVWDVLSNEEVVEIVNSTSTRSSAARMVVDAAAREWKLKYPTSKMD 374
            LT+ D FI+LA+DGVWDVLSN+E V+IV +  +R++AAR +VD A R W+LK+PTSK D
Sbjct: 334 RLTEKDEFIILATDGVWDVLSNKEAVDIVAAAPSRATAARALVDCAVRSWRLKFPTSKSD 393

Query: 375 DCAVICLFLD----GKMDSESENEEQASSAALQTNQMGNNTAD--------SDEEQRSEP 434
           DCAV+CLFLD      +  E+E+EE+ +      + +     D        S EEQ +EP
Sbjct: 394 DCAVVCLFLDHAKSPDLIQENESEEETTEDVAIPDTVAKVDQDIAQGDAHISSEEQITEP 453

Query: 435 SLQRNNTVRSSEENDQI--GGLSSHGEENGETMSA-------EDENWLGLEGVTRVNSLV 469
           +LQ + T+R  +E   +    +S   E  G   S        E+E W  LEGVTRVNSL+
Sbjct: 454 ALQHSYTLRDVDEIVPVEEPPVSKEPERCGSARSLADCISTNEEEEWSALEGVTRVNSLL 513

BLAST of MS004827 vs. ExPASy Swiss-Prot
Match: Q9M8R7 (Probable protein phosphatase 2C 33 OS=Arabidopsis thaliana OX=3702 GN=PPC6-1 PE=1 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 7.2e-110
Identity = 234/501 (46.71%), Postives = 322/501 (64.27%), Query Frame = 0

Query: 1   MGSCLSTGYQSSCHGGSNGETVSGSCCLGIGVGGRIARKRTFSDHIFSLQH-----LNSM 60
           MGSCLS   +S   G          C     V  R   K+       S  +     LN +
Sbjct: 1   MGSCLSAESRSPRPG--------SPCSPAFSVRKRKNSKKRPGSRNSSFDYRREEPLNQV 60

Query: 61  PNRLVFNGKTRNSCIFTQQGRKGINQDAMVVWEDFIS-DGAVFCGIFDGHGPHGHVVARK 120
           P R+  NG T  +CI+TQQG+KG NQDAMVVWE+F S    +FCG+FDGHGP+GH+VA++
Sbjct: 61  PGRMFLNGSTEVACIYTQQGKKGPNQDAMVVWENFGSRTDTIFCGVFDGHGPYGHMVAKR 120

Query: 121 VRDVLPRKLVSFWQS-----SQLK-------------HNESDRSSCFGWGRGNSADGEGL 180
           VRD LP KL ++W++       LK             +N  D ++   +    +A+ E  
Sbjct: 121 VRDNLPLKLSAYWEAKVPVEGVLKAITTDTVNNVTNINNPEDAAAAAAF---VTAEEEPR 180

Query: 181 ETAGSEEDKSSS------LWRRAFLKSYKSMDKELKSHPKLDCFCSGSTAVTLVKQGSDL 240
            +A  EE+ + +        + +FLK++K MD+ELK H  +DCFCSG+TAVTL+KQG  L
Sbjct: 181 TSADMEEENTETQPELFQTLKESFLKAFKVMDRELKFHGSVDCFCSGTTAVTLIKQGQYL 240

Query: 241 FVGSIGDSRAILGSKDSCDSLVATQLTVDLKPDLPREAERIKKCKGRVFALQDEPEVPRV 300
            VG++GDSRA++G++DS ++LVA QLTVDLKP+LP EAERI+KC+GRVFAL+DEPEV RV
Sbjct: 241 VVGNVGDSRAVMGTRDSENTLVAVQLTVDLKPNLPAEAERIRKCRGRVFALRDEPEVCRV 300

Query: 301 WLPFDNAPGLAMARAFGDFCLKDYGVISIPEFSHRLLTDYDLFIVLASDGVWDVLSNEEV 360
           WLP  ++PGLAMARAFGDFCLKD+G+IS+P+ S R LT+ D FIVLA+DG+WDVLSNE+V
Sbjct: 301 WLPNCDSPGLAMARAFGDFCLKDFGLISVPDVSFRQLTEKDEFIVLATDGIWDVLSNEDV 360

Query: 361 VEIVNSTSTRSSAARMVVDAAAREWKLKYPTSKMDDCAVICLFLDGKMDSESENEEQASS 420
           V IV S  +RSSAAR +V++A R W+ KYPTSK+DDCA +CL+LD    + +     +S 
Sbjct: 361 VAIVASAPSRSSAARALVESAVRAWRYKYPTSKVDDCAAVCLYLDS--SNTNAISTASSI 420

Query: 421 AALQTNQMGNNTADSDEEQRSEPS-LQRNNTVRSSEENDQIGGLSSHGEENGETMSAE-D 470
           + L+  +     A ++++  S PS L R++TVRS +E       +    +  + + +E  
Sbjct: 421 SKLEDGEEEELKATTEDDDASGPSGLGRSSTVRSGKEIALDESETEKLIKEADNLDSEPG 480

BLAST of MS004827 vs. ExPASy TrEMBL
Match: A0A6J1CGY6 (probable protein phosphatase 2C 52 OS=Momordica charantia OX=3673 GN=LOC111011326 PE=4 SV=1)

HSP 1 Score: 932.2 bits (2408), Expect = 8.7e-268
Identity = 467/470 (99.36%), Postives = 468/470 (99.57%), Query Frame = 0

Query: 1   MGSCLSTGYQSSCHGGSNGETVSGSCCLGIGVGGRIARKRTFSDHIFSLQHLNSMPNRLV 60
           MGSCLSTGYQSSCHGGSNGETVSGSCCLGIGVGGRIARK TFSDHIFSLQHLNS+PNRLV
Sbjct: 1   MGSCLSTGYQSSCHGGSNGETVSGSCCLGIGVGGRIARKITFSDHIFSLQHLNSIPNRLV 60

Query: 61  FNGKTRNSCIFTQQGRKGINQDAMVVWEDFISDGAVFCGIFDGHGPHGHVVARKVRDVLP 120
           FNGKTRNSCIFTQQGRKGINQDAMVVWEDFISDGAVFCGIFDGHGPHGHVVARKVRDVLP
Sbjct: 61  FNGKTRNSCIFTQQGRKGINQDAMVVWEDFISDGAVFCGIFDGHGPHGHVVARKVRDVLP 120

Query: 121 RKLVSFWQSSQLKHNESDRSSCFGWGRGNSADGEGLETAGSEEDKSSSLWRRAFLKSYKS 180
           RKLVSFWQSSQLKHN SDRSSCFGWGRGNSADGEGLETAGSEEDKSSSLWRRAFLKSYKS
Sbjct: 121 RKLVSFWQSSQLKHNGSDRSSCFGWGRGNSADGEGLETAGSEEDKSSSLWRRAFLKSYKS 180

Query: 181 MDKELKSHPKLDCFCSGSTAVTLVKQGSDLFVGSIGDSRAILGSKDSCDSLVATQLTVDL 240
           MDKELKSHPKLDCFCSGSTAVTLVKQGSDLFVGSIGDSRAILGSKDSCDSLVATQLTVDL
Sbjct: 181 MDKELKSHPKLDCFCSGSTAVTLVKQGSDLFVGSIGDSRAILGSKDSCDSLVATQLTVDL 240

Query: 241 KPDLPREAERIKKCKGRVFALQDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP 300
           KPDLPREAERIKKCKGRVFALQDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP
Sbjct: 241 KPDLPREAERIKKCKGRVFALQDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP 300

Query: 301 EFSHRLLTDYDLFIVLASDGVWDVLSNEEVVEIVNSTSTRSSAARMVVDAAAREWKLKYP 360
           EFSHRLLTDYDLFIVLASDGVWDVLSNEEVVEIVNSTSTRSSAARMVVDAAAREWKLKYP
Sbjct: 301 EFSHRLLTDYDLFIVLASDGVWDVLSNEEVVEIVNSTSTRSSAARMVVDAAAREWKLKYP 360

Query: 361 TSKMDDCAVICLFLDGKMDSESENEEQASSAALQTNQMGNNTADSDEEQRSEPSLQRNNT 420
           TSKMDDCAVICLFLDGKMDSESENEEQASSAALQTNQMGNNTADSDEEQRSEPSLQRNNT
Sbjct: 361 TSKMDDCAVICLFLDGKMDSESENEEQASSAALQTNQMGNNTADSDEEQRSEPSLQRNNT 420

Query: 421 VRSSEENDQIGGLSSHGEENGETMSAEDENWLGLEGVTRVNSLVQLPRFS 471
           VRSSEENDQIGGLSSHGEENGETMSAEDENWLGLEGVTRVNSLVQLPRFS
Sbjct: 421 VRSSEENDQIGGLSSHGEENGETMSAEDENWLGLEGVTRVNSLVQLPRFS 470

BLAST of MS004827 vs. ExPASy TrEMBL
Match: A0A6J1FV19 (probable protein phosphatase 2C 52 OS=Cucurbita moschata OX=3662 GN=LOC111449060 PE=4 SV=1)

HSP 1 Score: 800.4 bits (2066), Expect = 3.9e-228
Identity = 408/471 (86.62%), Postives = 432/471 (91.72%), Query Frame = 0

Query: 1   MGSCLSTGYQSSCHGGSNGETVSGSCCLGIGVGGRIARKRTFSDHIFSLQHLNSMPNRLV 60
           MGSCLS+G QSSC+GGSNGE V  S CLG GV GRI R RTFSDH+FSLQHLNS+PNRLV
Sbjct: 1   MGSCLSSGNQSSCNGGSNGEGVFES-CLGNGVCGRIERNRTFSDHVFSLQHLNSVPNRLV 60

Query: 61  FNGKTRNSCIFTQQGRKGINQDAMVVWEDFISDGAVFCGIFDGHGPHGHVVARKVRDVLP 120
           +NGKT NSCIFTQQGRKGINQDAMVVWEDFISDGA+FCG+FDGHGPHGHVVARKVRDVLP
Sbjct: 61  YNGKTSNSCIFTQQGRKGINQDAMVVWEDFISDGAIFCGVFDGHGPHGHVVARKVRDVLP 120

Query: 121 RKLVSFWQSSQLKHNESDRSSCFGWGRGNSADGEGLETAGSEEDKSSSLWRRAFLKSYKS 180
           RKLVSFWQSSQLK + SDR+SC  W R NSADGEG +  GS+ED+SSSLWRR FLKSYK+
Sbjct: 121 RKLVSFWQSSQLKRDRSDRTSCLAWSRKNSADGEGQDIDGSQEDRSSSLWRRTFLKSYKA 180

Query: 181 MDKELKSHPKLDCFCSGSTAVTLVKQGSDLFVGSIGDSRAILGSKDSCDSLVATQLTVDL 240
           MDKELKS PKLDCFCSG TA+TLVKQGSDLFVGSIGDSRAILGSKDS DSLVATQLTVDL
Sbjct: 181 MDKELKSDPKLDCFCSGCTAITLVKQGSDLFVGSIGDSRAILGSKDSSDSLVATQLTVDL 240

Query: 241 KPDLPREAERIKKCKGRVFALQDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP 300
           KPDLPREAERIKKCKGRVFAL DEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP
Sbjct: 241 KPDLPREAERIKKCKGRVFALHDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP 300

Query: 301 EFSHRLLTDYDLFIVLASDGVWDVLSNEEVVEIVNSTSTRSSAARMVVDAAAREWKLKYP 360
           EFSHR LTD DLF+VLASDGVWDVLSN+EVVEIV S STRSSAARMVVD+AAREWKLKYP
Sbjct: 301 EFSHRALTDNDLFVVLASDGVWDVLSNDEVVEIVYSASTRSSAARMVVDSAAREWKLKYP 360

Query: 361 TSKMDDCAVICLFLDGKMDSESENEEQASSAALQTNQMGNNTADSDEEQRS-EPSLQRNN 420
           TSKMDDCAV+CLFLDGKMDSESENEEQAS A L++NQM +NT  SDEEQRS EPSLQRN 
Sbjct: 361 TSKMDDCAVVCLFLDGKMDSESENEEQASCATLESNQM-SNTECSDEEQRSGEPSLQRNY 420

Query: 421 TVRSSEENDQIGGLSSHGEENGETMSAEDENWLGLEGVTRVNSLVQLPRFS 471
           TVRSSEE D+IGGL SH EEN E+MSAEDE+WLGLEGVTRVNSLVQLPRFS
Sbjct: 421 TVRSSEEKDRIGGLYSHSEENEESMSAEDESWLGLEGVTRVNSLVQLPRFS 469

BLAST of MS004827 vs. ExPASy TrEMBL
Match: A0A6J1JG54 (probable protein phosphatase 2C 52 OS=Cucurbita maxima OX=3661 GN=LOC111484091 PE=4 SV=1)

HSP 1 Score: 795.8 bits (2054), Expect = 9.7e-227
Identity = 406/471 (86.20%), Postives = 430/471 (91.30%), Query Frame = 0

Query: 1   MGSCLSTGYQSSCHGGSNGETVSGSCCLGIGVGGRIARKRTFSDHIFSLQHLNSMPNRLV 60
           MGSCLS+G QSSC+GGSNGE V  S CLGIGV GRI R RTFSDH+FSLQHLNS+PNRLV
Sbjct: 1   MGSCLSSGNQSSCNGGSNGEGVFES-CLGIGVCGRIERNRTFSDHVFSLQHLNSVPNRLV 60

Query: 61  FNGKTRNSCIFTQQGRKGINQDAMVVWEDFISDGAVFCGIFDGHGPHGHVVARKVRDVLP 120
           +NGKT NSCIFTQQGRKGINQDAMVVWEDFISDGA+FCG+FDGHGPHGHVVARKVRDVLP
Sbjct: 61  YNGKTSNSCIFTQQGRKGINQDAMVVWEDFISDGAIFCGVFDGHGPHGHVVARKVRDVLP 120

Query: 121 RKLVSFWQSSQLKHNESDRSSCFGWGRGNSADGEGLETAGSEEDKSSSLWRRAFLKSYKS 180
           RKLVSFWQSSQ K + SDR+SC  W R NSADGEG +  GS+ED+SS LWRR FLKSYK+
Sbjct: 121 RKLVSFWQSSQSKRDRSDRTSCLAWSRKNSADGEGQDIDGSQEDRSSYLWRRTFLKSYKA 180

Query: 181 MDKELKSHPKLDCFCSGSTAVTLVKQGSDLFVGSIGDSRAILGSKDSCDSLVATQLTVDL 240
           MDKELKS PKLDCFCSG TA+TLVKQGSDLFVGSIGDSRAILGSKDS DSLVATQLTVDL
Sbjct: 181 MDKELKSDPKLDCFCSGCTAITLVKQGSDLFVGSIGDSRAILGSKDSSDSLVATQLTVDL 240

Query: 241 KPDLPREAERIKKCKGRVFALQDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP 300
           KPDLPREAERIKKCKGRVFAL DEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP
Sbjct: 241 KPDLPREAERIKKCKGRVFALHDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP 300

Query: 301 EFSHRLLTDYDLFIVLASDGVWDVLSNEEVVEIVNSTSTRSSAARMVVDAAAREWKLKYP 360
           EFSHR+LTD DLF+VLASDGVWDVLSN+EVVEIV S  TRS AARMVVD AAR+WKLKYP
Sbjct: 301 EFSHRVLTDNDLFVVLASDGVWDVLSNDEVVEIVYSALTRSLAARMVVDLAARQWKLKYP 360

Query: 361 TSKMDDCAVICLFLDGKMDSESENEEQASSAALQTNQMGNNTADSDEEQRS-EPSLQRNN 420
           TSKMDDCAV+CLFLDGKMDSESENEEQASSA L++NQM +NT  SDEEQRS EPSLQRN 
Sbjct: 361 TSKMDDCAVVCLFLDGKMDSESENEEQASSATLESNQM-SNTECSDEEQRSGEPSLQRNY 420

Query: 421 TVRSSEENDQIGGLSSHGEENGETMSAEDENWLGLEGVTRVNSLVQLPRFS 471
           TVRSSEE D+IGGL SH EEN E+MSAEDENWLGLEGVTRVNSLVQLPRFS
Sbjct: 421 TVRSSEEKDRIGGLYSHREENEESMSAEDENWLGLEGVTRVNSLVQLPRFS 469

BLAST of MS004827 vs. ExPASy TrEMBL
Match: A0A6J1I6F2 (probable protein phosphatase 2C 52 OS=Cucurbita maxima OX=3661 GN=LOC111469610 PE=4 SV=1)

HSP 1 Score: 790.8 bits (2041), Expect = 3.1e-225
Identity = 398/470 (84.68%), Postives = 432/470 (91.91%), Query Frame = 0

Query: 1   MGSCLSTGYQSSCHGGSNGETVSGSCCLGIGVGGRIARKRTFSDHIFSLQHLNSMPNRLV 60
           MG CLSTG Q SC+GG+NGE +SGS CLGI V GRI RKRTFSDH FS+QHLNS+PNRLV
Sbjct: 1   MGICLSTGNQCSCNGGTNGERISGS-CLGIRVSGRIERKRTFSDHTFSIQHLNSIPNRLV 60

Query: 61  FNGKTRNSCIFTQQGRKGINQDAMVVWEDFISDGAVFCGIFDGHGPHGHVVARKVRDVLP 120
           +NGKTRNSCIFTQQGRKGINQDAMVVWEDFISDGA+FCG+FDGHGPHGHVVARKVRDVLP
Sbjct: 61  YNGKTRNSCIFTQQGRKGINQDAMVVWEDFISDGAIFCGVFDGHGPHGHVVARKVRDVLP 120

Query: 121 RKLVSFWQSSQLKHNESDRSSCFGWGRGNSADGEGLETAGSEEDKSSSLWRRAFLKSYKS 180
           RKL S W+SSQLKHN +DR+SCF W + NSADGE  +  GS+EDKSSSLWR+AFLKSYK+
Sbjct: 121 RKLESSWESSQLKHNRTDRTSCFRWSQKNSADGERHDMDGSQEDKSSSLWRKAFLKSYKA 180

Query: 181 MDKELKSHPKLDCFCSGSTAVTLVKQGSDLFVGSIGDSRAILGSKDSCDSLVATQLTVDL 240
           MDKELKSHPK+DCFCSG TAVTLVKQGSDLFVGSIGDSRAILGSKDS DS VATQLTVDL
Sbjct: 181 MDKELKSHPKVDCFCSGCTAVTLVKQGSDLFVGSIGDSRAILGSKDSRDSPVATQLTVDL 240

Query: 241 KPDLPREAERIKKCKGRVFALQDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP 300
           KPDLPREAERIKKCKGRVFALQDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP
Sbjct: 241 KPDLPREAERIKKCKGRVFALQDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP 300

Query: 301 EFSHRLLTDYDLFIVLASDGVWDVLSNEEVVEIVNSTSTRSSAARMVVDAAAREWKLKYP 360
           EFSHR+LTD DLF+VLASDGVWDVLSN+EVVEIV+S  TRSSAARMVVD+AAREWKLKYP
Sbjct: 301 EFSHRVLTDNDLFVVLASDGVWDVLSNQEVVEIVHSAPTRSSAARMVVDSAAREWKLKYP 360

Query: 361 TSKMDDCAVICLFLDGKMDSESENEEQASSAALQTNQMGNNTADSDEEQRSEPSLQRNNT 420
           TSKMDDCAV+CLFLDGKMDS SENEEQ S A  QT++M ++T +SDEE+R EPSLQRNNT
Sbjct: 361 TSKMDDCAVVCLFLDGKMDSNSENEEQ-SLATFQTDEM-DDTENSDEERRLEPSLQRNNT 420

Query: 421 VRSSEENDQIGGLSSHGEENGETMSAEDENWLGLEGVTRVNSLVQLPRFS 471
           VRSSEE +QIGG SS  EENGE++ A+DENWLGLEGVTRVNSL+QLPRFS
Sbjct: 421 VRSSEEKEQIGGRSSQSEENGESICAKDENWLGLEGVTRVNSLIQLPRFS 467

BLAST of MS004827 vs. ExPASy TrEMBL
Match: A0A6J1G6T0 (probable protein phosphatase 2C 52 OS=Cucurbita moschata OX=3662 GN=LOC111451280 PE=4 SV=1)

HSP 1 Score: 789.3 bits (2037), Expect = 9.1e-225
Identity = 398/470 (84.68%), Postives = 431/470 (91.70%), Query Frame = 0

Query: 1   MGSCLSTGYQSSCHGGSNGETVSGSCCLGIGVGGRIARKRTFSDHIFSLQHLNSMPNRLV 60
           MG CLSTG Q SC+GG+NGE +SGS CLGI V GRI RKRTFSDH FS+QHLNS+PNRLV
Sbjct: 1   MGICLSTGNQCSCNGGANGERISGS-CLGIRVSGRIERKRTFSDHTFSIQHLNSIPNRLV 60

Query: 61  FNGKTRNSCIFTQQGRKGINQDAMVVWEDFISDGAVFCGIFDGHGPHGHVVARKVRDVLP 120
           +NGKTRNSCIFTQQGRKGINQDAMVVWEDFISDGA+FCG+FDGHGPHGHVVARKVRDVLP
Sbjct: 61  YNGKTRNSCIFTQQGRKGINQDAMVVWEDFISDGAIFCGVFDGHGPHGHVVARKVRDVLP 120

Query: 121 RKLVSFWQSSQLKHNESDRSSCFGWGRGNSADGEGLETAGSEEDKSSSLWRRAFLKSYKS 180
           RKL S W+SSQLKHN SDR+SCF W + NSADGE  +  GS+EDKSSSLWR+AFLKSYK+
Sbjct: 121 RKLESSWESSQLKHNRSDRTSCFRWSQKNSADGERHDMDGSQEDKSSSLWRKAFLKSYKA 180

Query: 181 MDKELKSHPKLDCFCSGSTAVTLVKQGSDLFVGSIGDSRAILGSKDSCDSLVATQLTVDL 240
           MDKELKSHPK+DCFCSG TAVTLVKQGSDLFVGSIGDSRAILGSKDS DS VATQLTVDL
Sbjct: 181 MDKELKSHPKVDCFCSGCTAVTLVKQGSDLFVGSIGDSRAILGSKDSSDSPVATQLTVDL 240

Query: 241 KPDLPREAERIKKCKGRVFALQDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP 300
           KPDLPREAERIKKCKGRVFALQDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP
Sbjct: 241 KPDLPREAERIKKCKGRVFALQDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP 300

Query: 301 EFSHRLLTDYDLFIVLASDGVWDVLSNEEVVEIVNSTSTRSSAARMVVDAAAREWKLKYP 360
           EFSHR+LTD DLF+VLASDGVWDVLSN+EVVEIV+S  TRSSAARMVVD+AAREWKLKYP
Sbjct: 301 EFSHRVLTDNDLFVVLASDGVWDVLSNQEVVEIVHSAPTRSSAARMVVDSAAREWKLKYP 360

Query: 361 TSKMDDCAVICLFLDGKMDSESENEEQASSAALQTNQMGNNTADSDEEQRSEPSLQRNNT 420
           TSKMDDCAV+CLFLDGKMDS SENEEQ S A LQT++M  +T +SDEEQR EPSLQRN+T
Sbjct: 361 TSKMDDCAVVCLFLDGKMDSNSENEEQ-SLATLQTDEM-YHTENSDEEQRLEPSLQRNDT 420

Query: 421 VRSSEENDQIGGLSSHGEENGETMSAEDENWLGLEGVTRVNSLVQLPRFS 471
           VRSSEE +QIGG  S  +ENGE++ A+DENWLGLEGVTRVNSL+QLPRFS
Sbjct: 421 VRSSEEKEQIGGRCSQSKENGESICAKDENWLGLEGVTRVNSLIQLPRFS 467

BLAST of MS004827 vs. TAIR 10
Match: AT4G03415.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 607.8 bits (1566), Expect = 7.2e-174
Identity = 317/472 (67.16%), Postives = 379/472 (80.30%), Query Frame = 0

Query: 1   MGSCLSTGYQSSCHGGSNGETVSGSCCLGIGVGGRIARKRTFSDHIFSLQHLNSMPNRLV 60
           MG C+ST  +S+C   SNGE       LGIG       KRTFSDHI SLQ+L S+PNR+ 
Sbjct: 1   MGGCVSTSSKSTCSSWSNGEKPVRRPYLGIGCCVSKRAKRTFSDHIVSLQNLTSIPNRIT 60

Query: 61  FNGKTRNSCIFTQQGRKGINQDAMVVWEDFISDGAVFCGIFDGHGPHGHVVARKVRDVLP 120
            + K+R+SCIFTQQGRKGINQDAM+VWEDF+S+   FCG+FDGHGP+GH+VARKVRD LP
Sbjct: 61  SSSKSRSSCIFTQQGRKGINQDAMIVWEDFMSEDVTFCGVFDGHGPYGHLVARKVRDTLP 120

Query: 121 RKLVSFWQSSQLKHNESDRSSCFGWGRGNSADGEGLETAGSEEDKSSSLWRRAFLKSYKS 180
            KL  F+Q+ Q K N S + + F      SA  E ++  GS+EDK   LW  AFLKS+K+
Sbjct: 121 VKLQFFFQTLQSKQNCS-KGTRFRRNSSKSAVQEAVK-EGSDEDKLKGLWGEAFLKSFKA 180

Query: 181 MDKELKSHPKLDCFCSGSTAVTLVKQGSDLFVGSIGDSRAILGSKDSCDSLVATQLTVDL 240
           MDKEL+SHP LDCFCSGST VT++KQGS+LF+G+IGDSRAILGSKDS DS+VATQLTVDL
Sbjct: 181 MDKELRSHPNLDCFCSGSTGVTILKQGSNLFMGNIGDSRAILGSKDSNDSMVATQLTVDL 240

Query: 241 KPDLPREAERIKKCKGRVFALQDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP 300
           KPDLPREAERIK+CKGRVFA++DEPEVPRVWLP+D+APGLAMARAFGDFCLK+YGVIS+P
Sbjct: 241 KPDLPREAERIKRCKGRVFAMEDEPEVPRVWLPYDDAPGLAMARAFGDFCLKEYGVISVP 300

Query: 301 EFSHRLLTDYDLFIVLASDGVWDVLSNEEVVEIVNSTSTRSSAARMVVDAAAREWKLKYP 360
           EF+HR+LTD D FIVLASDGVWDVLSNEEVV+IV S ++R+SAAR +V++AAREWKLKYP
Sbjct: 301 EFTHRVLTDRDQFIVLASDGVWDVLSNEEVVDIVASATSRASAARTLVNSAAREWKLKYP 360

Query: 361 TSKMDDCAVICLFLDGKMDSESENEEQASSAALQTNQMGNNTADSDEEQRSEPSLQRNNT 420
           TSKMDDCAV+CLFLDGKMDSES+ +EQ  S+A        N  +SD+ QRSEP LQRN T
Sbjct: 361 TSKMDDCAVVCLFLDGKMDSESDYDEQGFSSA-------TNAVESDDGQRSEPCLQRNFT 420

Query: 421 VRSS--EENDQIGGLSSHGEENGETMSAEDENWLGLEGVTRVNSLVQLPRFS 471
           VRSS  +EN+  G +++  +   E  +  D+NWLGL+GVTRVNSLVQLPRFS
Sbjct: 421 VRSSSDQENETYGNVNTETDAEDE-KTVGDQNWLGLQGVTRVNSLVQLPRFS 462

BLAST of MS004827 vs. TAIR 10
Match: AT4G03415.2 (Protein phosphatase 2C family protein )

HSP 1 Score: 607.8 bits (1566), Expect = 7.2e-174
Identity = 317/472 (67.16%), Postives = 379/472 (80.30%), Query Frame = 0

Query: 1   MGSCLSTGYQSSCHGGSNGETVSGSCCLGIGVGGRIARKRTFSDHIFSLQHLNSMPNRLV 60
           MG C+ST  +S+C   SNGE       LGIG       KRTFSDHI SLQ+L S+PNR+ 
Sbjct: 1   MGGCVSTSSKSTCSSWSNGEKPVRRPYLGIGCCVSKRAKRTFSDHIVSLQNLTSIPNRIT 60

Query: 61  FNGKTRNSCIFTQQGRKGINQDAMVVWEDFISDGAVFCGIFDGHGPHGHVVARKVRDVLP 120
            + K+R+SCIFTQQGRKGINQDAM+VWEDF+S+   FCG+FDGHGP+GH+VARKVRD LP
Sbjct: 61  SSSKSRSSCIFTQQGRKGINQDAMIVWEDFMSEDVTFCGVFDGHGPYGHLVARKVRDTLP 120

Query: 121 RKLVSFWQSSQLKHNESDRSSCFGWGRGNSADGEGLETAGSEEDKSSSLWRRAFLKSYKS 180
            KL  F+Q+ Q K N S + + F      SA  E ++  GS+EDK   LW  AFLKS+K+
Sbjct: 121 VKLQFFFQTLQSKQNCS-KGTRFRRNSSKSAVQEAVK-EGSDEDKLKGLWGEAFLKSFKA 180

Query: 181 MDKELKSHPKLDCFCSGSTAVTLVKQGSDLFVGSIGDSRAILGSKDSCDSLVATQLTVDL 240
           MDKEL+SHP LDCFCSGST VT++KQGS+LF+G+IGDSRAILGSKDS DS+VATQLTVDL
Sbjct: 181 MDKELRSHPNLDCFCSGSTGVTILKQGSNLFMGNIGDSRAILGSKDSNDSMVATQLTVDL 240

Query: 241 KPDLPREAERIKKCKGRVFALQDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP 300
           KPDLPREAERIK+CKGRVFA++DEPEVPRVWLP+D+APGLAMARAFGDFCLK+YGVIS+P
Sbjct: 241 KPDLPREAERIKRCKGRVFAMEDEPEVPRVWLPYDDAPGLAMARAFGDFCLKEYGVISVP 300

Query: 301 EFSHRLLTDYDLFIVLASDGVWDVLSNEEVVEIVNSTSTRSSAARMVVDAAAREWKLKYP 360
           EF+HR+LTD D FIVLASDGVWDVLSNEEVV+IV S ++R+SAAR +V++AAREWKLKYP
Sbjct: 301 EFTHRVLTDRDQFIVLASDGVWDVLSNEEVVDIVASATSRASAARTLVNSAAREWKLKYP 360

Query: 361 TSKMDDCAVICLFLDGKMDSESENEEQASSAALQTNQMGNNTADSDEEQRSEPSLQRNNT 420
           TSKMDDCAV+CLFLDGKMDSES+ +EQ  S+A        N  +SD+ QRSEP LQRN T
Sbjct: 361 TSKMDDCAVVCLFLDGKMDSESDYDEQGFSSA-------TNAVESDDGQRSEPCLQRNFT 420

Query: 421 VRSS--EENDQIGGLSSHGEENGETMSAEDENWLGLEGVTRVNSLVQLPRFS 471
           VRSS  +EN+  G +++  +   E  +  D+NWLGL+GVTRVNSLVQLPRFS
Sbjct: 421 VRSSSDQENETYGNVNTETDAEDE-KTVGDQNWLGLQGVTRVNSLVQLPRFS 462

BLAST of MS004827 vs. TAIR 10
Match: AT1G03590.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 577.4 bits (1487), Expect = 1.0e-164
Identity = 307/474 (64.77%), Postives = 361/474 (76.16%), Query Frame = 0

Query: 1   MGSCLSTGYQSSCHGGSNGETVSGSCCLGIGVGGRIARKRTFSDHIFSLQHLNSMPNRLV 60
           MG C+S    S+       E      CLG+G  G    KR FSD + SL +L S+PNR++
Sbjct: 1   MGGCISKTSWSN-------EEPMHRPCLGMGCCGSKMGKRGFSDRMVSLHNLVSIPNRII 60

Query: 61  FNGKTRNSCIFTQQGRKGINQDAMVVWEDFISDGAVFCGIFDGHGPHGHVVARKVRDVLP 120
            NGK+R+SCIFTQQGRKGINQDAM+VWEDF+S    FCG+FDGHGPHGH+VARKVRD LP
Sbjct: 61  GNGKSRSSCIFTQQGRKGINQDAMIVWEDFMSKDVTFCGVFDGHGPHGHLVARKVRDSLP 120

Query: 121 RKLVSFWQSSQLKHNESDRSSCFGWGRGNSADGEGLETAGSEEDKSSSLWRRAFLKSYKS 180
            KL+S   S + K N        G     S   E  +   +EEDK + LW  AFLKS+ +
Sbjct: 121 VKLLSLLNSIKSKQN-----GPIGTRASKSDSLEAEKEESTEEDKLNFLWEEAFLKSFNA 180

Query: 181 MDKELKSHPKLDCFCSGSTAVTLVKQGSDLFVGSIGDSRAILGSKDSCDSLVATQLTVDL 240
           MDKEL+SHP L+CFCSG TAVT++KQGS+L++G+IGDSRAILGSKDS DS++A QLTVDL
Sbjct: 181 MDKELRSHPNLECFCSGCTAVTIIKQGSNLYMGNIGDSRAILGSKDSNDSMIAVQLTVDL 240

Query: 241 KPDLPREAERIKKCKGRVFALQDEPEVPRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP 300
           KPDLPREAERIK+CKGRVFALQDEPEV RVWLPFDNAPGLAMARAFGDFCLKDYGVISIP
Sbjct: 241 KPDLPREAERIKQCKGRVFALQDEPEVSRVWLPFDNAPGLAMARAFGDFCLKDYGVISIP 300

Query: 301 EFSHRLLTDYDLFIVLASDGVWDVLSNEEVVEIVNSTSTRSSAARMVVDAAAREWKLKYP 360
           EFSHR+LTD D FIVLASDGVWDVLSNEEVVE+V S ++R+SAAR+VVD+A REWKLKYP
Sbjct: 301 EFSHRVLTDRDQFIVLASDGVWDVLSNEEVVEVVASATSRASAARLVVDSAVREWKLKYP 360

Query: 361 TSKMDDCAVICLFLDGKMDSE-SENEEQASSAALQTNQMGNNTADSDEEQRSEPSLQRNN 420
           TSKMDDCAV+CLFLDG+MDSE S+NEEQ  S+A        N  +SDE Q +EP LQRN 
Sbjct: 361 TSKMDDCAVVCLFLDGRMDSETSDNEEQCFSSA-------TNAVESDESQGAEPCLQRNV 420

Query: 421 TVRS---SEENDQIGGLSSHGEENGETMSAE-DENWLGLEGVTRVNSLVQLPRF 470
           TVRS    +EN+  G + +  +   +  + E ++NW GLEGVTRVNSLVQLPRF
Sbjct: 421 TVRSLSTDQENNSYGKVIAEADNAEKEKTREGEQNWSGLEGVTRVNSLVQLPRF 455

BLAST of MS004827 vs. TAIR 10
Match: AT3G02750.1 (Protein phosphatase 2C family protein )

HSP 1 Score: 399.1 bits (1024), Expect = 5.1e-111
Identity = 234/501 (46.71%), Postives = 322/501 (64.27%), Query Frame = 0

Query: 1   MGSCLSTGYQSSCHGGSNGETVSGSCCLGIGVGGRIARKRTFSDHIFSLQH-----LNSM 60
           MGSCLS   +S   G          C     V  R   K+       S  +     LN +
Sbjct: 1   MGSCLSAESRSPRPG--------SPCSPAFSVRKRKNSKKRPGSRNSSFDYRREEPLNQV 60

Query: 61  PNRLVFNGKTRNSCIFTQQGRKGINQDAMVVWEDFIS-DGAVFCGIFDGHGPHGHVVARK 120
           P R+  NG T  +CI+TQQG+KG NQDAMVVWE+F S    +FCG+FDGHGP+GH+VA++
Sbjct: 61  PGRMFLNGSTEVACIYTQQGKKGPNQDAMVVWENFGSRTDTIFCGVFDGHGPYGHMVAKR 120

Query: 121 VRDVLPRKLVSFWQS-----SQLK-------------HNESDRSSCFGWGRGNSADGEGL 180
           VRD LP KL ++W++       LK             +N  D ++   +    +A+ E  
Sbjct: 121 VRDNLPLKLSAYWEAKVPVEGVLKAITTDTVNNVTNINNPEDAAAAAAF---VTAEEEPR 180

Query: 181 ETAGSEEDKSSS------LWRRAFLKSYKSMDKELKSHPKLDCFCSGSTAVTLVKQGSDL 240
            +A  EE+ + +        + +FLK++K MD+ELK H  +DCFCSG+TAVTL+KQG  L
Sbjct: 181 TSADMEEENTETQPELFQTLKESFLKAFKVMDRELKFHGSVDCFCSGTTAVTLIKQGQYL 240

Query: 241 FVGSIGDSRAILGSKDSCDSLVATQLTVDLKPDLPREAERIKKCKGRVFALQDEPEVPRV 300
            VG++GDSRA++G++DS ++LVA QLTVDLKP+LP EAERI+KC+GRVFAL+DEPEV RV
Sbjct: 241 VVGNVGDSRAVMGTRDSENTLVAVQLTVDLKPNLPAEAERIRKCRGRVFALRDEPEVCRV 300

Query: 301 WLPFDNAPGLAMARAFGDFCLKDYGVISIPEFSHRLLTDYDLFIVLASDGVWDVLSNEEV 360
           WLP  ++PGLAMARAFGDFCLKD+G+IS+P+ S R LT+ D FIVLA+DG+WDVLSNE+V
Sbjct: 301 WLPNCDSPGLAMARAFGDFCLKDFGLISVPDVSFRQLTEKDEFIVLATDGIWDVLSNEDV 360

Query: 361 VEIVNSTSTRSSAARMVVDAAAREWKLKYPTSKMDDCAVICLFLDGKMDSESENEEQASS 420
           V IV S  +RSSAAR +V++A R W+ KYPTSK+DDCA +CL+LD    + +     +S 
Sbjct: 361 VAIVASAPSRSSAARALVESAVRAWRYKYPTSKVDDCAAVCLYLDS--SNTNAISTASSI 420

Query: 421 AALQTNQMGNNTADSDEEQRSEPS-LQRNNTVRSSEENDQIGGLSSHGEENGETMSAE-D 470
           + L+  +     A ++++  S PS L R++TVRS +E       +    +  + + +E  
Sbjct: 421 SKLEDGEEEELKATTEDDDASGPSGLGRSSTVRSGKEIALDESETEKLIKEADNLDSEPG 480

BLAST of MS004827 vs. TAIR 10
Match: AT3G02750.2 (Protein phosphatase 2C family protein )

HSP 1 Score: 399.1 bits (1024), Expect = 5.1e-111
Identity = 234/501 (46.71%), Postives = 322/501 (64.27%), Query Frame = 0

Query: 1   MGSCLSTGYQSSCHGGSNGETVSGSCCLGIGVGGRIARKRTFSDHIFSLQH-----LNSM 60
           MGSCLS   +S   G          C     V  R   K+       S  +     LN +
Sbjct: 1   MGSCLSAESRSPRPG--------SPCSPAFSVRKRKNSKKRPGSRNSSFDYRREEPLNQV 60

Query: 61  PNRLVFNGKTRNSCIFTQQGRKGINQDAMVVWEDFIS-DGAVFCGIFDGHGPHGHVVARK 120
           P R+  NG T  +CI+TQQG+KG NQDAMVVWE+F S    +FCG+FDGHGP+GH+VA++
Sbjct: 61  PGRMFLNGSTEVACIYTQQGKKGPNQDAMVVWENFGSRTDTIFCGVFDGHGPYGHMVAKR 120

Query: 121 VRDVLPRKLVSFWQS-----SQLK-------------HNESDRSSCFGWGRGNSADGEGL 180
           VRD LP KL ++W++       LK             +N  D ++   +    +A+ E  
Sbjct: 121 VRDNLPLKLSAYWEAKVPVEGVLKAITTDTVNNVTNINNPEDAAAAAAF---VTAEEEPR 180

Query: 181 ETAGSEEDKSSS------LWRRAFLKSYKSMDKELKSHPKLDCFCSGSTAVTLVKQGSDL 240
            +A  EE+ + +        + +FLK++K MD+ELK H  +DCFCSG+TAVTL+KQG  L
Sbjct: 181 TSADMEEENTETQPELFQTLKESFLKAFKVMDRELKFHGSVDCFCSGTTAVTLIKQGQYL 240

Query: 241 FVGSIGDSRAILGSKDSCDSLVATQLTVDLKPDLPREAERIKKCKGRVFALQDEPEVPRV 300
            VG++GDSRA++G++DS ++LVA QLTVDLKP+LP EAERI+KC+GRVFAL+DEPEV RV
Sbjct: 241 VVGNVGDSRAVMGTRDSENTLVAVQLTVDLKPNLPAEAERIRKCRGRVFALRDEPEVCRV 300

Query: 301 WLPFDNAPGLAMARAFGDFCLKDYGVISIPEFSHRLLTDYDLFIVLASDGVWDVLSNEEV 360
           WLP  ++PGLAMARAFGDFCLKD+G+IS+P+ S R LT+ D FIVLA+DG+WDVLSNE+V
Sbjct: 301 WLPNCDSPGLAMARAFGDFCLKDFGLISVPDVSFRQLTEKDEFIVLATDGIWDVLSNEDV 360

Query: 361 VEIVNSTSTRSSAARMVVDAAAREWKLKYPTSKMDDCAVICLFLDGKMDSESENEEQASS 420
           V IV S  +RSSAAR +V++A R W+ KYPTSK+DDCA +CL+LD    + +     +S 
Sbjct: 361 VAIVASAPSRSSAARALVESAVRAWRYKYPTSKVDDCAAVCLYLDS--SNTNAISTASSI 420

Query: 421 AALQTNQMGNNTADSDEEQRSEPS-LQRNNTVRSSEENDQIGGLSSHGEENGETMSAE-D 470
           + L+  +     A ++++  S PS L R++TVRS +E       +    +  + + +E  
Sbjct: 421 SKLEDGEEEELKATTEDDDASGPSGLGRSSTVRSGKEIALDESETEKLIKEADNLDSEPG 480

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022140736.11.8e-26799.36probable protein phosphatase 2C 52 [Momordica charantia][more]
XP_038902772.11.4e-23289.38probable protein phosphatase 2C 52 [Benincasa hispida][more]
KAG7010695.14.3e-22986.84putative protein phosphatase 2C 52 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022944671.18.1e-22886.62probable protein phosphatase 2C 52 [Cucurbita moschata][more]
XP_023513362.16.9e-22786.86probable protein phosphatase 2C 52 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q8GY601.0e-17267.16Probable protein phosphatase 2C 52 OS=Arabidopsis thaliana OX=3702 GN=At4g03415 ... [more]
Q9LR651.5e-16364.77Probable protein phosphatase 2C 1 OS=Arabidopsis thaliana OX=3702 GN=PPC6-6 PE=1... [more]
Q8H4S62.6e-12852.85Probable protein phosphatase 2C 64 OS=Oryza sativa subsp. japonica OX=39947 GN=O... [more]
Q6ZKL84.2e-11849.59Probable protein phosphatase 2C 66 OS=Oryza sativa subsp. japonica OX=39947 GN=O... [more]
Q9M8R77.2e-11046.71Probable protein phosphatase 2C 33 OS=Arabidopsis thaliana OX=3702 GN=PPC6-1 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1CGY68.7e-26899.36probable protein phosphatase 2C 52 OS=Momordica charantia OX=3673 GN=LOC11101132... [more]
A0A6J1FV193.9e-22886.62probable protein phosphatase 2C 52 OS=Cucurbita moschata OX=3662 GN=LOC111449060... [more]
A0A6J1JG549.7e-22786.20probable protein phosphatase 2C 52 OS=Cucurbita maxima OX=3661 GN=LOC111484091 P... [more]
A0A6J1I6F23.1e-22584.68probable protein phosphatase 2C 52 OS=Cucurbita maxima OX=3661 GN=LOC111469610 P... [more]
A0A6J1G6T09.1e-22584.68probable protein phosphatase 2C 52 OS=Cucurbita moschata OX=3662 GN=LOC111451280... [more]
Match NameE-valueIdentityDescription
AT4G03415.17.2e-17467.16Protein phosphatase 2C family protein [more]
AT4G03415.27.2e-17467.16Protein phosphatase 2C family protein [more]
AT1G03590.11.0e-16464.77Protein phosphatase 2C family protein [more]
AT3G02750.15.1e-11146.71Protein phosphatase 2C family protein [more]
AT3G02750.25.1e-11146.71Protein phosphatase 2C family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001932PPM-type phosphatase domainSMARTSM00332PP2C_4coord: 56..372
e-value: 3.0E-70
score: 249.4
IPR001932PPM-type phosphatase domainPFAMPF00481PP2Ccoord: 164..351
e-value: 1.1E-37
score: 130.1
IPR001932PPM-type phosphatase domainPROSITEPS51746PPM_2coord: 67..374
score: 45.737564
IPR001932PPM-type phosphatase domainCDDcd00143PP2Cccoord: 73..374
e-value: 1.72551E-68
score: 217.194
IPR036457PPM-type phosphatase domain superfamilyGENE3D3.60.40.10coord: 57..376
e-value: 2.4E-74
score: 252.5
IPR036457PPM-type phosphatase domain superfamilySUPERFAMILY81606PP2C-likecoord: 58..376
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 414..440
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 382..453
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 385..404
NoneNo IPR availablePANTHERPTHR47992:SF62PROTEIN PHOSPHATASE 2C 52-RELATEDcoord: 1..462
NoneNo IPR availablePANTHERPTHR47992ALPHABET, ISOFORM E-RELATEDcoord: 1..462

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS004827.1MS004827.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016791 phosphatase activity