MS004821 (gene) Bitter gourd (TR) v1

BLAST of MS004821 vs. NCBI nr
Match: XP_022140355.1 (IST1-like protein isoform X3 [Momordica charantia])

HSP 1 Score: 926.0 bits (2392), Expect = 1.4e-265
Identity = 470/481 (97.71%), Postives = 472/481 (98.13%), Query Frame = 0

Query: 17  KKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVEQIVKDESRMVAYEI 76
           KKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVEQIVKDESRMVAYEI
Sbjct: 15  KKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVEQIVKDESRMVAYEI 74

Query: 77  LGNFCEFILQNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQLIRKLFGERYGRRF 136
           LGNFCEFILQNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQLIRKLFGERYGRRF
Sbjct: 75  LGNFCEFILQNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQLIRKLFGERYGRRF 134

Query: 137 ETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARDCLQPQLLALEYRSDWHQKQ 196
           ETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARDC QPQLLALEYRSDWHQKQ
Sbjct: 135 ETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARDCFQPQLLALEYRSDWHQKQ 194

Query: 197 VALIL--TIHSDERKREKALLKREKALLLHGYSARSTSCDTLPQFPEERIVHVDDVVELC 256
           V   +   IHSDERKREKALLKREKALLLHGYSARSTSCDTLPQFPEERIVHVDDVVELC
Sbjct: 195 VEFPIKQAIHSDERKREKALLKREKALLLHGYSARSTSCDTLPQFPEERIVHVDDVVELC 254

Query: 257 SSTTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHADPSDSWSEIENSSSKGSGKGSKRI 316
           SSTTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHADPSDSWSEIENSSSKGSGKGSKRI
Sbjct: 255 SSTTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHADPSDSWSEIENSSSKGSGKGSKRI 314

Query: 317 SLNKGMKKTEHNNEKGNNCHEISEQKQRKTENWVSEGATDKEMEWASFYKKPRRRRRRKP 376
           SLNKGMKKTEHNNEKGNNCHEISEQKQRKTENWVSEGATDKEMEWASFYKKPRRRRRRKP
Sbjct: 315 SLNKGMKKTEHNNEKGNNCHEISEQKQRKTENWVSEGATDKEMEWASFYKKPRRRRRRKP 374

Query: 377 PPLRLPSSDLKSAAYDIFTYPGYGSNTETKKLKETRKSNAVDNGDDKNVSSYPVRNKKEA 436
           PPLRLP SDLKSAAYD+FTYPGYGSNTETKKLKETRKSNAVDNGDDKNVSSYPVRNKKEA
Sbjct: 375 PPLRLPCSDLKSAAYDVFTYPGYGSNTETKKLKETRKSNAVDNGDDKNVSSYPVRNKKEA 434

Query: 437 LYLRAATFPPKQSPKASFTRTNSCPYKQPSHVHPKLPDYDDIAAKFMALKREHLQKNTPK 496
           LYLRAATFPPKQSPKA FTRTNSCPYKQPSHVHPKLPDYDDIAAKFMALKREHLQKNTPK
Sbjct: 435 LYLRAATFPPKQSPKACFTRTNSCPYKQPSHVHPKLPDYDDIAAKFMALKREHLQKNTPK 494

BLAST of MS004821 vs. NCBI nr
Match: XP_022140353.1 (IST1-like protein isoform X1 [Momordica charantia])

HSP 1 Score: 919.5 bits (2375), Expect = 1.3e-263
Identity = 470/487 (96.51%), Postives = 472/487 (96.92%), Query Frame = 0

Query: 17  KKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVEQIVKDESRMVAYEI 76
           KKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVEQIVKDESRMVAYEI
Sbjct: 15  KKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVEQIVKDESRMVAYEI 74

Query: 77  LGNFCEFILQNLSYIRKHK------DCPNDVNEAVSSLMFASARCGDLPELQLIRKLFGE 136
           LGNFCEFILQNLSYIRKHK      DCPNDVNEAVSSLMFASARCGDLPELQLIRKLFGE
Sbjct: 75  LGNFCEFILQNLSYIRKHKLVFGFRDCPNDVNEAVSSLMFASARCGDLPELQLIRKLFGE 134

Query: 137 RYGRRFETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARDCLQPQLLALEYRS 196
           RYGRRFETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARDC QPQLLALEYRS
Sbjct: 135 RYGRRFETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARDCFQPQLLALEYRS 194

Query: 197 DWHQKQVALIL--TIHSDERKREKALLKREKALLLHGYSARSTSCDTLPQFPEERIVHVD 256
           DWHQKQV   +   IHSDERKREKALLKREKALLLHGYSARSTSCDTLPQFPEERIVHVD
Sbjct: 195 DWHQKQVEFPIKQAIHSDERKREKALLKREKALLLHGYSARSTSCDTLPQFPEERIVHVD 254

Query: 257 DVVELCSSTTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHADPSDSWSEIENSSSKGSG 316
           DVVELCSSTTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHADPSDSWSEIENSSSKGSG
Sbjct: 255 DVVELCSSTTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHADPSDSWSEIENSSSKGSG 314

Query: 317 KGSKRISLNKGMKKTEHNNEKGNNCHEISEQKQRKTENWVSEGATDKEMEWASFYKKPRR 376
           KGSKRISLNKGMKKTEHNNEKGNNCHEISEQKQRKTENWVSEGATDKEMEWASFYKKPRR
Sbjct: 315 KGSKRISLNKGMKKTEHNNEKGNNCHEISEQKQRKTENWVSEGATDKEMEWASFYKKPRR 374

Query: 377 RRRRKPPPLRLPSSDLKSAAYDIFTYPGYGSNTETKKLKETRKSNAVDNGDDKNVSSYPV 436
           RRRRKPPPLRLP SDLKSAAYD+FTYPGYGSNTETKKLKETRKSNAVDNGDDKNVSSYPV
Sbjct: 375 RRRRKPPPLRLPCSDLKSAAYDVFTYPGYGSNTETKKLKETRKSNAVDNGDDKNVSSYPV 434

Query: 437 RNKKEALYLRAATFPPKQSPKASFTRTNSCPYKQPSHVHPKLPDYDDIAAKFMALKREHL 496
           RNKKEALYLRAATFPPKQSPKA FTRTNSCPYKQPSHVHPKLPDYDDIAAKFMALKREHL
Sbjct: 435 RNKKEALYLRAATFPPKQSPKACFTRTNSCPYKQPSHVHPKLPDYDDIAAKFMALKREHL 494

BLAST of MS004821 vs. NCBI nr
Match: XP_022140354.1 (uncharacterized protein LOC111011049 isoform X2 [Momordica charantia])

HSP 1 Score: 909.1 bits (2348), Expect = 1.7e-260
Identity = 467/487 (95.89%), Postives = 469/487 (96.30%), Query Frame = 0

Query: 17  KKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVEQIVKDESRMVAYEI 76
           KKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNR   IVKDESRMVAYEI
Sbjct: 15  KKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNR---IVKDESRMVAYEI 74

Query: 77  LGNFCEFILQNLSYIRKHK------DCPNDVNEAVSSLMFASARCGDLPELQLIRKLFGE 136
           LGNFCEFILQNLSYIRKHK      DCPNDVNEAVSSLMFASARCGDLPELQLIRKLFGE
Sbjct: 75  LGNFCEFILQNLSYIRKHKLVFGFRDCPNDVNEAVSSLMFASARCGDLPELQLIRKLFGE 134

Query: 137 RYGRRFETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARDCLQPQLLALEYRS 196
           RYGRRFETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARDC QPQLLALEYRS
Sbjct: 135 RYGRRFETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARDCFQPQLLALEYRS 194

Query: 197 DWHQKQVALIL--TIHSDERKREKALLKREKALLLHGYSARSTSCDTLPQFPEERIVHVD 256
           DWHQKQV   +   IHSDERKREKALLKREKALLLHGYSARSTSCDTLPQFPEERIVHVD
Sbjct: 195 DWHQKQVEFPIKQAIHSDERKREKALLKREKALLLHGYSARSTSCDTLPQFPEERIVHVD 254

Query: 257 DVVELCSSTTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHADPSDSWSEIENSSSKGSG 316
           DVVELCSSTTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHADPSDSWSEIENSSSKGSG
Sbjct: 255 DVVELCSSTTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHADPSDSWSEIENSSSKGSG 314

Query: 317 KGSKRISLNKGMKKTEHNNEKGNNCHEISEQKQRKTENWVSEGATDKEMEWASFYKKPRR 376
           KGSKRISLNKGMKKTEHNNEKGNNCHEISEQKQRKTENWVSEGATDKEMEWASFYKKPRR
Sbjct: 315 KGSKRISLNKGMKKTEHNNEKGNNCHEISEQKQRKTENWVSEGATDKEMEWASFYKKPRR 374

Query: 377 RRRRKPPPLRLPSSDLKSAAYDIFTYPGYGSNTETKKLKETRKSNAVDNGDDKNVSSYPV 436
           RRRRKPPPLRLP SDLKSAAYD+FTYPGYGSNTETKKLKETRKSNAVDNGDDKNVSSYPV
Sbjct: 375 RRRRKPPPLRLPCSDLKSAAYDVFTYPGYGSNTETKKLKETRKSNAVDNGDDKNVSSYPV 434

Query: 437 RNKKEALYLRAATFPPKQSPKASFTRTNSCPYKQPSHVHPKLPDYDDIAAKFMALKREHL 496
           RNKKEALYLRAATFPPKQSPKA FTRTNSCPYKQPSHVHPKLPDYDDIAAKFMALKREHL
Sbjct: 435 RNKKEALYLRAATFPPKQSPKACFTRTNSCPYKQPSHVHPKLPDYDDIAAKFMALKREHL 494

BLAST of MS004821 vs. NCBI nr
Match: XP_038901982.1 (uncharacterized protein LOC120088637 [Benincasa hispida])

HSP 1 Score: 549.3 bits (1414), Expect = 3.4e-152
Identity = 315/503 (62.62%), Postives = 360/503 (71.57%), Query Frame = 0

Query: 3   GLSFFDLGLSAGCSKKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVE 62
           G  FF    ++ C KKLIKQVQCRLKLL NKKSVI KQLRED+++L +NGY QTAFNRVE
Sbjct: 3   GFVFFGWRKASKC-KKLIKQVQCRLKLLNNKKSVITKQLREDIVQLFQNGYHQTAFNRVE 62

Query: 63  QIVKDESRMVAYEILGNFCEFILQNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQ 122
           Q++KDE+RM AYEIL NFCEFIL NLSYIRKHKDCPNDVNEAVSSL+FASARCGDLPEL 
Sbjct: 63  QVLKDETRMAAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLLFASARCGDLPELH 122

Query: 123 LIRKLFGERYGRRFETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARDCLQPQ 182
           LIRKLFGERYGR FET+AVEL+PGNLVN QIKEKLS  SVS+DDK RMINEI RD LQP+
Sbjct: 123 LIRKLFGERYGRNFETTAVELNPGNLVNLQIKEKLSITSVSDDDKQRMINEILRDRLQPE 182

Query: 183 LLALEYRSDWHQKQVALILTIHSDE-----RKREKALLKREKALLLHGYSARSTSCDTLP 242
            LALEYRSDWHQ QV      H DE     +K+     + +K  L +  S  STS + LP
Sbjct: 183 FLALEYRSDWHQNQV------HDDEKAEQHKKQAVNADETKKEDLHYSNSVTSTSSECLP 242

Query: 243 QFPEERIVHVDDVVELCSSTTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHADPSDSWS 302
           QFPEERIV++DDVVELCSSTTTEGDQ LFKFKT     + + + ++  NQI    S+SWS
Sbjct: 243 QFPEERIVYLDDVVELCSSTTTEGDQRLFKFKTTPTLSKREISKENYHNQIDVIQSESWS 302

Query: 303 EIENSSSKGSGKGSKRISLNKGMKKTEHNNEKGNNCHEISEQKQRKTEN-WVSEGATDKE 362
           + ENSSSK S +GSK+  + +G  KTE++N+   + HE S  KQ K EN W SE  TDKE
Sbjct: 303 DNENSSSKTSVEGSKKRFVTEGKPKTENDNDIEKDNHEKSPWKQGKRENYWASEETTDKE 362

Query: 363 MEWASFYKKPRRRRRRKPPPLRLPSSDLKSAAYDIFTYPGYGSNTETKKLKETRKSNAVD 422
            EWA+FYKKPRRRRRR+      PSSDLK   YD     GY S+   KK++   +   V 
Sbjct: 363 KEWANFYKKPRRRRRRRKSRESPPSSDLKFTTYD-----GYNSSINNKKVEANCQKMDV- 422

Query: 423 NGDDKNVSSYPVRNKKEALYLRAATFP---PKQSPK-ASFTRTNSCPYKQPSHVHPKLPD 482
                         KKE LYLRA T P   PK+  K  SF RT SCPYKQPSHVHPKLPD
Sbjct: 423 --------------KKEGLYLRAVTMPRERPKERQKEVSFARTISCPYKQPSHVHPKLPD 478

Query: 483 YDDIAAKFMALKREHLQKNTPKP 496
           YDDIAAKF+ALKRE LQ NT KP
Sbjct: 483 YDDIAAKFIALKRERLQNNTLKP 478

BLAST of MS004821 vs. NCBI nr
Match: XP_023534324.1 (uncharacterized protein LOC111795916 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 548.9 bits (1413), Expect = 4.5e-152
Identity = 312/493 (63.29%), Postives = 364/493 (73.83%), Query Frame = 0

Query: 6   FFDLGLSAGCSKKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVEQIV 65
           FF    ++ C KKLIKQVQCRLKLLKNKK +I KQ++EDV++LI+NGY+QTAFNRVEQIV
Sbjct: 5   FFGWRKASKC-KKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVEQIV 64

Query: 66  KDESRMVAYEILGNFCEFILQNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQLIR 125
           KDE+R+ AYEIL NFCEFIL NLSYIRKHKDCPNDVNEAVSSLMFA+ARCGDLPELQ IR
Sbjct: 65  KDENRVAAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQHIR 124

Query: 126 KLFGERYGRRFETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARDCLQPQLLA 185
           KLFGERYGRRFE++AV+L PGNLVN QIKEKL  NSVS+DDK RM+NEIARDCLQPQLLA
Sbjct: 125 KLFGERYGRRFESAAVQLGPGNLVNCQIKEKLLINSVSDDDKQRMMNEIARDCLQPQLLA 184

Query: 186 LEYRSDWHQKQVALILTIHSDERKREKALLKREKALLLHGYSARSTSCDTLPQFPEERIV 245
           L+YRSDWHQ QV   +   +DE+  +          +    S  S+SCD+LPQFPEERIV
Sbjct: 185 LQYRSDWHQNQVVARIA-KADEKPDQH---------INQANSGTSSSCDSLPQFPEERIV 244

Query: 246 HVDDVVELCSSTTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHADPSDSWSEIENSSSK 305
           ++DDVVELCSST  EGDQ LFKFKTAA  P+ +   +HSSNQ   DP++S ++IENSSSK
Sbjct: 245 YLDDVVELCSSTNIEGDQRLFKFKTAATLPKTEIT-QHSSNQTDTDPTESRTDIENSSSK 304

Query: 306 GSGKGSKRISLNKGMKKTEHNNEKGNNCHEISEQKQRKTENWVSEGATDKEMEWASFYKK 365
            S KGS        M     +  KG N   +            SEGATDKEMEWASFYKK
Sbjct: 305 ISVKGS--------MGTEGKHQIKGKNFTTVQNG---------SEGATDKEMEWASFYKK 364

Query: 366 PRRRRRRKPPPLRLP-SSDLKSAAYDIFTYPGYGSNTETKKLKETRKSNAVDNGDDKNVS 425
           P RRR ++     LP SSDL S  YD+FTY     N   K      KSNA++N ++  +S
Sbjct: 365 PGRRRTKRS---ELPSSSDLSSTTYDVFTY-----NGNKKVAANDTKSNAMENCEEMKLS 424

Query: 426 SYPVRNKKEALYLRAATFP---PKQSPKASFTRTNSCPYKQPSHVHPKLPDYDDIAAKFM 485
           S  +RNK+EALYLRA T P   PK+S K +F RTNSCP K+P+HVHPKLPDYDDIAAKFM
Sbjct: 425 S-SLRNKREALYLRAETMPTERPKESQKVNFCRTNSCPDKKPTHVHPKLPDYDDIAAKFM 459

Query: 486 ALKREHLQKNTPK 495
           AL+R+HLQ NT K
Sbjct: 485 ALRRQHLQNNTLK 459

BLAST of MS004821 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 4.7e-11
Identity = 46/159 (28.93%), Postives = 86/159 (54.09%), Query Frame = 0

Query: 17  KKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVEQIVKDESRMVAYEI 76
           K  +K    R+++LKNKK+ I +  + +V EL++   E++A  RVE I++DE  +  ++I
Sbjct: 14  KVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRDEYLIECFQI 73

Query: 77  LGNFCEFILQNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQLIRKLFGERYGRRF 136
           +   CE +   ++ I    + P ++ E++ +L+++S R   +PEL+ I+     +YG+  
Sbjct: 74  IEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQLKAKYGKGL 133

Query: 137 ETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIA 176
           E  A        VN +I  KLS  +       + ++EIA
Sbjct: 134 ENEA-NCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIA 170

BLAST of MS004821 vs. ExPASy Swiss-Prot
Match: Q3ZBV1 (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 6.8e-10
Identity = 46/173 (26.59%), Postives = 96/173 (55.49%), Query Frame = 0

Query: 10  GLSAGCSKKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVEQIVKDES 69
           G+ A   +  ++ V  RLKLL+ KK+ +A++ R+++ + +  G ++ A  RVE I++++ 
Sbjct: 5   GIKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDY 64

Query: 70  RMVAYEILGNFCEFILQNLSYIRKHKDCPNDVNEAVSSLMFASARC-GDLPELQLIR--- 129
            + A EIL  +C+ +L     I+  K+  + + E+VS+L++A+ R   ++ EL+++    
Sbjct: 65  LVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQL 124

Query: 130 -KLFGERYGRRFETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARD 178
              + + YG+   T+ +       VN ++  KLS  +  +    R + EIA++
Sbjct: 125 CAKYSKEYGKLCRTNQI-----GTVNDRLMHKLSVEAPPKILVERYLIEIAKN 172

BLAST of MS004821 vs. ExPASy Swiss-Prot
Match: P53990 (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 8.9e-10
Identity = 43/157 (27.39%), Postives = 89/157 (56.69%), Query Frame = 0

Query: 26  RLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVEQIVKDESRMVAYEILGNFCEFIL 85
           RLKLL+ KK+ +A++ R+++ + +  G ++ A  RVE I++++  + A EIL  +C+ +L
Sbjct: 21  RLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILELYCDLLL 80

Query: 86  QNLSYIRKHKDCPNDVNEAVSSLMFASARC-GDLPELQLIR----KLFGERYGRRFETSA 145
                I+  K+  + + E+VS+L++A+ R   ++ EL+++       + + YG+   T+ 
Sbjct: 81  ARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKLCRTNQ 140

Query: 146 VELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARD 178
           +       VN ++  KLS  +  +    R + EIA++
Sbjct: 141 I-----GTVNDRLMHKLSVEAPPKILVERYLIEIAKN 172

BLAST of MS004821 vs. ExPASy Swiss-Prot
Match: Q9CX00 (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 8.9e-10
Identity = 43/157 (27.39%), Postives = 89/157 (56.69%), Query Frame = 0

Query: 26  RLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVEQIVKDESRMVAYEILGNFCEFIL 85
           RLKLL+ KK+ +A++ R+++ + +  G ++ A  RVE I++++  + A EIL  +C+ +L
Sbjct: 21  RLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILELYCDLLL 80

Query: 86  QNLSYIRKHKDCPNDVNEAVSSLMFASARC-GDLPELQLIR----KLFGERYGRRFETSA 145
                I+  K+  + + E+VS+L++A+ R   ++ EL+++       + + YG+   T+ 
Sbjct: 81  ARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKLCRTNQ 140

Query: 146 VELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARD 178
           +       VN ++  KLS  +  +    R + EIA++
Sbjct: 141 I-----GTVNDRLMHKLSVEAPPKILVERYLIEIAKN 172

BLAST of MS004821 vs. ExPASy Swiss-Prot
Match: Q568Z6 (IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 8.9e-10
Identity = 43/157 (27.39%), Postives = 89/157 (56.69%), Query Frame = 0

Query: 26  RLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVEQIVKDESRMVAYEILGNFCEFIL 85
           RLKLL+ KK+ +A++ R+++ + +  G ++ A  RVE I++++  + A EIL  +C+ +L
Sbjct: 21  RLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILELYCDLLL 80

Query: 86  QNLSYIRKHKDCPNDVNEAVSSLMFASARC-GDLPELQLIR----KLFGERYGRRFETSA 145
                I+  K+  + + E+VS+L++A+ R   ++ EL+++       + + YG+   T+ 
Sbjct: 81  ARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKLCRTNQ 140

Query: 146 VELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARD 178
           +       VN ++  KLS  +  +    R + EIA++
Sbjct: 141 I-----GTVNDRLMHKLSVEAPPKILVERYLIEIAKN 172

BLAST of MS004821 vs. ExPASy TrEMBL
Match: A0A6J1CEV2 (IST1-like protein isoform X3 OS=Momordica charantia OX=3673 GN=LOC111011049 PE=3 SV=1)

HSP 1 Score: 926.0 bits (2392), Expect = 6.5e-266
Identity = 470/481 (97.71%), Postives = 472/481 (98.13%), Query Frame = 0

Query: 17  KKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVEQIVKDESRMVAYEI 76
           KKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVEQIVKDESRMVAYEI
Sbjct: 15  KKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVEQIVKDESRMVAYEI 74

Query: 77  LGNFCEFILQNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQLIRKLFGERYGRRF 136
           LGNFCEFILQNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQLIRKLFGERYGRRF
Sbjct: 75  LGNFCEFILQNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQLIRKLFGERYGRRF 134

Query: 137 ETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARDCLQPQLLALEYRSDWHQKQ 196
           ETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARDC QPQLLALEYRSDWHQKQ
Sbjct: 135 ETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARDCFQPQLLALEYRSDWHQKQ 194

Query: 197 VALIL--TIHSDERKREKALLKREKALLLHGYSARSTSCDTLPQFPEERIVHVDDVVELC 256
           V   +   IHSDERKREKALLKREKALLLHGYSARSTSCDTLPQFPEERIVHVDDVVELC
Sbjct: 195 VEFPIKQAIHSDERKREKALLKREKALLLHGYSARSTSCDTLPQFPEERIVHVDDVVELC 254

Query: 257 SSTTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHADPSDSWSEIENSSSKGSGKGSKRI 316
           SSTTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHADPSDSWSEIENSSSKGSGKGSKRI
Sbjct: 255 SSTTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHADPSDSWSEIENSSSKGSGKGSKRI 314

Query: 317 SLNKGMKKTEHNNEKGNNCHEISEQKQRKTENWVSEGATDKEMEWASFYKKPRRRRRRKP 376
           SLNKGMKKTEHNNEKGNNCHEISEQKQRKTENWVSEGATDKEMEWASFYKKPRRRRRRKP
Sbjct: 315 SLNKGMKKTEHNNEKGNNCHEISEQKQRKTENWVSEGATDKEMEWASFYKKPRRRRRRKP 374

Query: 377 PPLRLPSSDLKSAAYDIFTYPGYGSNTETKKLKETRKSNAVDNGDDKNVSSYPVRNKKEA 436
           PPLRLP SDLKSAAYD+FTYPGYGSNTETKKLKETRKSNAVDNGDDKNVSSYPVRNKKEA
Sbjct: 375 PPLRLPCSDLKSAAYDVFTYPGYGSNTETKKLKETRKSNAVDNGDDKNVSSYPVRNKKEA 434

Query: 437 LYLRAATFPPKQSPKASFTRTNSCPYKQPSHVHPKLPDYDDIAAKFMALKREHLQKNTPK 496
           LYLRAATFPPKQSPKA FTRTNSCPYKQPSHVHPKLPDYDDIAAKFMALKREHLQKNTPK
Sbjct: 435 LYLRAATFPPKQSPKACFTRTNSCPYKQPSHVHPKLPDYDDIAAKFMALKREHLQKNTPK 494

BLAST of MS004821 vs. ExPASy TrEMBL
Match: A0A6J1CHT9 (IST1-like protein isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011049 PE=3 SV=1)

HSP 1 Score: 919.5 bits (2375), Expect = 6.1e-264
Identity = 470/487 (96.51%), Postives = 472/487 (96.92%), Query Frame = 0

Query: 17  KKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVEQIVKDESRMVAYEI 76
           KKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVEQIVKDESRMVAYEI
Sbjct: 15  KKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVEQIVKDESRMVAYEI 74

Query: 77  LGNFCEFILQNLSYIRKHK------DCPNDVNEAVSSLMFASARCGDLPELQLIRKLFGE 136
           LGNFCEFILQNLSYIRKHK      DCPNDVNEAVSSLMFASARCGDLPELQLIRKLFGE
Sbjct: 75  LGNFCEFILQNLSYIRKHKLVFGFRDCPNDVNEAVSSLMFASARCGDLPELQLIRKLFGE 134

Query: 137 RYGRRFETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARDCLQPQLLALEYRS 196
           RYGRRFETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARDC QPQLLALEYRS
Sbjct: 135 RYGRRFETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARDCFQPQLLALEYRS 194

Query: 197 DWHQKQVALIL--TIHSDERKREKALLKREKALLLHGYSARSTSCDTLPQFPEERIVHVD 256
           DWHQKQV   +   IHSDERKREKALLKREKALLLHGYSARSTSCDTLPQFPEERIVHVD
Sbjct: 195 DWHQKQVEFPIKQAIHSDERKREKALLKREKALLLHGYSARSTSCDTLPQFPEERIVHVD 254

Query: 257 DVVELCSSTTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHADPSDSWSEIENSSSKGSG 316
           DVVELCSSTTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHADPSDSWSEIENSSSKGSG
Sbjct: 255 DVVELCSSTTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHADPSDSWSEIENSSSKGSG 314

Query: 317 KGSKRISLNKGMKKTEHNNEKGNNCHEISEQKQRKTENWVSEGATDKEMEWASFYKKPRR 376
           KGSKRISLNKGMKKTEHNNEKGNNCHEISEQKQRKTENWVSEGATDKEMEWASFYKKPRR
Sbjct: 315 KGSKRISLNKGMKKTEHNNEKGNNCHEISEQKQRKTENWVSEGATDKEMEWASFYKKPRR 374

Query: 377 RRRRKPPPLRLPSSDLKSAAYDIFTYPGYGSNTETKKLKETRKSNAVDNGDDKNVSSYPV 436
           RRRRKPPPLRLP SDLKSAAYD+FTYPGYGSNTETKKLKETRKSNAVDNGDDKNVSSYPV
Sbjct: 375 RRRRKPPPLRLPCSDLKSAAYDVFTYPGYGSNTETKKLKETRKSNAVDNGDDKNVSSYPV 434

Query: 437 RNKKEALYLRAATFPPKQSPKASFTRTNSCPYKQPSHVHPKLPDYDDIAAKFMALKREHL 496
           RNKKEALYLRAATFPPKQSPKA FTRTNSCPYKQPSHVHPKLPDYDDIAAKFMALKREHL
Sbjct: 435 RNKKEALYLRAATFPPKQSPKACFTRTNSCPYKQPSHVHPKLPDYDDIAAKFMALKREHL 494

BLAST of MS004821 vs. ExPASy TrEMBL
Match: A0A6J1CFH7 (uncharacterized protein LOC111011049 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011049 PE=3 SV=1)

HSP 1 Score: 909.1 bits (2348), Expect = 8.3e-261
Identity = 467/487 (95.89%), Postives = 469/487 (96.30%), Query Frame = 0

Query: 17  KKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVEQIVKDESRMVAYEI 76
           KKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNR   IVKDESRMVAYEI
Sbjct: 15  KKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNR---IVKDESRMVAYEI 74

Query: 77  LGNFCEFILQNLSYIRKHK------DCPNDVNEAVSSLMFASARCGDLPELQLIRKLFGE 136
           LGNFCEFILQNLSYIRKHK      DCPNDVNEAVSSLMFASARCGDLPELQLIRKLFGE
Sbjct: 75  LGNFCEFILQNLSYIRKHKLVFGFRDCPNDVNEAVSSLMFASARCGDLPELQLIRKLFGE 134

Query: 137 RYGRRFETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARDCLQPQLLALEYRS 196
           RYGRRFETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARDC QPQLLALEYRS
Sbjct: 135 RYGRRFETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARDCFQPQLLALEYRS 194

Query: 197 DWHQKQVALIL--TIHSDERKREKALLKREKALLLHGYSARSTSCDTLPQFPEERIVHVD 256
           DWHQKQV   +   IHSDERKREKALLKREKALLLHGYSARSTSCDTLPQFPEERIVHVD
Sbjct: 195 DWHQKQVEFPIKQAIHSDERKREKALLKREKALLLHGYSARSTSCDTLPQFPEERIVHVD 254

Query: 257 DVVELCSSTTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHADPSDSWSEIENSSSKGSG 316
           DVVELCSSTTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHADPSDSWSEIENSSSKGSG
Sbjct: 255 DVVELCSSTTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHADPSDSWSEIENSSSKGSG 314

Query: 317 KGSKRISLNKGMKKTEHNNEKGNNCHEISEQKQRKTENWVSEGATDKEMEWASFYKKPRR 376
           KGSKRISLNKGMKKTEHNNEKGNNCHEISEQKQRKTENWVSEGATDKEMEWASFYKKPRR
Sbjct: 315 KGSKRISLNKGMKKTEHNNEKGNNCHEISEQKQRKTENWVSEGATDKEMEWASFYKKPRR 374

Query: 377 RRRRKPPPLRLPSSDLKSAAYDIFTYPGYGSNTETKKLKETRKSNAVDNGDDKNVSSYPV 436
           RRRRKPPPLRLP SDLKSAAYD+FTYPGYGSNTETKKLKETRKSNAVDNGDDKNVSSYPV
Sbjct: 375 RRRRKPPPLRLPCSDLKSAAYDVFTYPGYGSNTETKKLKETRKSNAVDNGDDKNVSSYPV 434

Query: 437 RNKKEALYLRAATFPPKQSPKASFTRTNSCPYKQPSHVHPKLPDYDDIAAKFMALKREHL 496
           RNKKEALYLRAATFPPKQSPKA FTRTNSCPYKQPSHVHPKLPDYDDIAAKFMALKREHL
Sbjct: 435 RNKKEALYLRAATFPPKQSPKACFTRTNSCPYKQPSHVHPKLPDYDDIAAKFMALKREHL 494

BLAST of MS004821 vs. ExPASy TrEMBL
Match: A0A6J1I3N6 (uncharacterized protein LOC111469611 OS=Cucurbita maxima OX=3661 GN=LOC111469611 PE=3 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 5.9e-150
Identity = 308/493 (62.47%), Postives = 363/493 (73.63%), Query Frame = 0

Query: 6   FFDLGLSAGCSKKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVEQIV 65
           FF    ++ C KKLIKQVQCRLKLLKNKK +I KQ++EDV++LI+NGY+QTAFNRVEQIV
Sbjct: 5   FFGWRKASKC-KKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFNRVEQIV 64

Query: 66  KDESRMVAYEILGNFCEFILQNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQLIR 125
           KDE+R+ AYEIL NFCEFIL NLSYIRKHKDCPNDVNEAVSSLMFA+ARCGDLPELQ IR
Sbjct: 65  KDENRVAAYEILDNFCEFILLNLSYIRKHKDCPNDVNEAVSSLMFAAARCGDLPELQHIR 124

Query: 126 KLFGERYGRRFETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARDCLQPQLLA 185
           KLFGERYGRRFE++AV+L PGNLVN QIKEKL  N VS+DDK RM+NEIARDCLQP+LLA
Sbjct: 125 KLFGERYGRRFESAAVQLGPGNLVNCQIKEKLLINPVSDDDKQRMMNEIARDCLQPELLA 184

Query: 186 LEYRSDWHQKQVALILTIHSDERKREKALLKREKALLLHGYSARSTSCDTLPQFPEERIV 245
           L+YRSDWHQ QV   +   +DE+  +          +    S  S+SCD+LPQFPEERIV
Sbjct: 185 LQYRSDWHQNQVVARIA-KADEKPDQH---------INQANSGTSSSCDSLPQFPEERIV 244

Query: 246 HVDDVVELCSSTTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHADPSDSWSEIENSSSK 305
           ++DDVVELCSST  EGDQ LFKFKTAA  P+ +   +H SNQ   DP++S ++IEN SSK
Sbjct: 245 YLDDVVELCSSTNIEGDQRLFKFKTAATLPKTEIT-QHRSNQTDTDPTESRTDIENPSSK 304

Query: 306 GSGKGSKRISLNKGMKKTEHNNEKGNNCHEISEQKQRKTENWVSEGATDKEMEWASFYKK 365
            S KGS                 +G   H+I  +     +N  SEGATDKEMEWASFYKK
Sbjct: 305 ISVKGSM--------------GTEGK--HQIKGENFTTVQNG-SEGATDKEMEWASFYKK 364

Query: 366 P-RRRRRRKPPPLRLPSSDLKSAAYDIFTYPGYGSNTETKKLKETRKSNAVDNGDDKNVS 425
           P RRR +R+  P    SSDL S  YD+FTY     N   K      KSNA++N ++  +S
Sbjct: 365 PGRRRTKRRELP---SSSDLSSTTYDVFTY-----NGNKKVAANDTKSNAMENCEEMKLS 424

Query: 426 SYPVRNKKEALYLRAATFP---PKQSPKASFTRTNSCPYKQPSHVHPKLPDYDDIAAKFM 485
           S  +RNK+EALYLRA T P   PK+S K +F RTNSCP K+P+HVHPKLPDYDDIAAKFM
Sbjct: 425 S-SLRNKREALYLRAETMPPERPKESQKVNFCRTNSCPDKKPTHVHPKLPDYDDIAAKFM 459

Query: 486 ALKREHLQKNTPK 495
           AL+R+HLQ NT K
Sbjct: 485 ALRRQHLQNNTLK 459

BLAST of MS004821 vs. ExPASy TrEMBL
Match: A0A6J1G7W6 (uncharacterized protein LOC111451538 OS=Cucurbita moschata OX=3662 GN=LOC111451538 PE=3 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 8.5e-149
Identity = 306/493 (62.07%), Postives = 361/493 (73.23%), Query Frame = 0

Query: 6   FFDLGLSAGCSKKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVEQIV 65
           FF    ++ C KKLIKQVQCRLKLLKNKK +I KQ++EDV++LI+NGY+QTAF+RVEQIV
Sbjct: 5   FFGWRKASKC-KKLIKQVQCRLKLLKNKKGIITKQMKEDVVQLIRNGYDQTAFSRVEQIV 64

Query: 66  KDESRMVAYEILGNFCEFILQNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQLIR 125
           KDE+R+ AYEIL NFCEFIL NLSYIRKH+DCPNDVNEAVSSLMFA+ARCGDLPELQ IR
Sbjct: 65  KDENRVAAYEILDNFCEFILLNLSYIRKHRDCPNDVNEAVSSLMFAAARCGDLPELQHIR 124

Query: 126 KLFGERYGRRFETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARDCLQPQLLA 185
           KLFGERYGRRFE+SAV+L PGNLVN QIKEKL  NSVS+DDK RM+NEIARDCLQPQLLA
Sbjct: 125 KLFGERYGRRFESSAVQLGPGNLVNCQIKEKLLINSVSDDDKQRMMNEIARDCLQPQLLA 184

Query: 186 LEYRSDWHQKQVALILTIHSDERKREKALLKREKALLLHGYSARSTSCDTLPQFPEERIV 245
           L+YRSDWHQ QV   +   +DE+  +          +    S  S+SCD+LPQFPEERIV
Sbjct: 185 LQYRSDWHQNQVVARIA-KADEKPDQH---------INQANSGTSSSCDSLPQFPEERIV 244

Query: 246 HVDDVVELCSSTTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHADPSDSWSEIENSSSK 305
           ++DDVVELCSST  EGDQ LFKFKTAA  P+ +   +H+SNQ   D ++S ++IEN SSK
Sbjct: 245 YLDDVVELCSSTNIEGDQRLFKFKTAATLPKTEIT-QHNSNQTDTDRTESRTDIENPSSK 304

Query: 306 GSGKGSKRISLNKGMKKTEHNNEKGNNCHEISEQKQRKTENWVSEGATDKEMEWASFYKK 365
            S KGS        M     +  KG N   +            SEGATDKEMEWASFYKK
Sbjct: 305 ISIKGS--------MGTEGKHQIKGKNFTTVQNG---------SEGATDKEMEWASFYKK 364

Query: 366 PRRRRRRKPPPLRLP-SSDLKSAAYDIFTYPGYGSNTETKKLKETRKSNAVDNGDDKNVS 425
           P RRR ++     LP SSDL S  YD+FTY     N   K      KSNA++N ++  + 
Sbjct: 365 PGRRRTKRS---ELPSSSDLSSTTYDVFTY-----NGNKKVAANDTKSNAMENCEEMKLP 424

Query: 426 SYPVRNKKEALYLRAATFP---PKQSPKASFTRTNSCPYKQPSHVHPKLPDYDDIAAKFM 485
           S  +RNK+EALYLRA T P   PK+S + +F RTNSCP K+P+HVHPKLPDYDDIAAKFM
Sbjct: 425 S-SLRNKREALYLRAETMPPERPKESQRVNFCRTNSCPDKKPTHVHPKLPDYDDIAAKFM 459

Query: 486 ALKREHLQKNTPK 495
           AL+R+HLQ NT K
Sbjct: 485 ALRRQHLQNNTLK 459

BLAST of MS004821 vs. TAIR 10
Match: AT2G14830.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 188.7 bits (478), Expect = 1.1e-47
Identity = 151/475 (31.79%), Postives = 217/475 (45.68%), Query Frame = 0

Query: 17  KKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVEQIVKDESRMVAYEI 76
           K ++KQ+QCRL LLKNKK  I+  LR D+ +L++ G    A +R +Q+  DE+ M  Y +
Sbjct: 85  KSVVKQLQCRLNLLKNKKYAISSHLRNDIAQLLRIGERDRALHRAQQLFLDENLMSLYHL 144

Query: 77  LGNFCEFILQNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQLIRKLFGERYGRRF 136
           L +F + IL NLSYIR+ +D P+ +NEAVS+L+FASARCGDLPEL+ +R LFG+RYG  F
Sbjct: 145 LLHFSDIILLNLSYIRRRRDLPDGINEAVSTLVFASARCGDLPELRALRVLFGKRYGNHF 204

Query: 137 ETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARDC-LQPQLLALEYRSDWHQK 196
             +A+ L PGN VN Q+ EKLS  SVS+D K +++ EI  +  L+ ++LA+EY  ++H  
Sbjct: 205 VDTALNLLPGNCVNPQVIEKLSIISVSDDAKSKLLGEIVEEYNLRLEVLAIEYTPEFH-- 264

Query: 197 QVALILTIHSDERKREKALLKREKALLLHGYSARSTSCDTLPQFPEERIVHVDDVVELCS 256
                           K +LK EKA                    E+ ++  +     CS
Sbjct: 265 ----------------KQVLKSEKA------------------EEEKEVMSSNSAQPYCS 324

Query: 257 STTTEGDQILFKFKTAAAFPEEDYNGKHSSNQIHADPSDSWSEIENSSSKGSGKGSKRIS 316
           S   E +  ++KF    A  EE+   +        D  D   E                 
Sbjct: 325 SQKAESEAEVYKFTLTDADIEENQEQERKV----CDEEDDCIE----------------- 384

Query: 317 LNKGMKKTEHNNEKGNNCHEISEQKQRKTENWVSEGATDKEMEWASFYKKPRRRRRRKPP 376
                              E+ E+ Q          +  +  E     +K R+R RRKP 
Sbjct: 385 ------------------EEVVEEDQ----------SVFRFRESTEDERKERKRLRRKP- 444

Query: 377 PLRLPSSDLKSAAYDIFTYPGYGSNTETKKLKETRKSNAVDNGDDKNVSSYPVRNKKEAL 436
             R  SS     A D+  +  Y     +++ KE  K              Y +       
Sbjct: 445 --RSTSSSSSPIAKDVDCWRYYYKGKRSRQRKECGK------------CCYHI------- 448

Query: 437 YLRAATFPPKQSPKASFTRTNSCPYKQPSHVHPKLPDYDDIAAKFMALKREHLQK 491
                T  P Q       R+    +K+  HVHPKLPDYD I A F AL+++  QK
Sbjct: 505 VYNVFTMLPDQKESEEGERS----FKKAMHVHPKLPDYDQIVAHFTALRKQQQQK 448

BLAST of MS004821 vs. TAIR 10
Match: AT1G34220.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 138.7 bits (348), Expect = 1.3e-32
Identity = 72/173 (41.62%), Postives = 108/173 (62.43%), Query Frame = 0

Query: 5   SFFDLGLSAGCSKKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVEQI 64
           SFF+ G  A   K L+K    R+KL++N++    KQ+R ++ +L++ G E TA  RVE I
Sbjct: 6   SFFNKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVEHI 65

Query: 65  VKDESRMVAYEILGNFCEFILQNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQLI 124
           +++E  M A EIL  FCE I   L  I   ++CP D+ EA+SS+ FA+ RC DL ELQ +
Sbjct: 66  IREEKMMAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQQV 125

Query: 125 RKLFGERYGRRFETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARD 178
           + LF  +YG+ F  +A EL P + VNR++ E LS  + S + K +++ EIA +
Sbjct: 126 QILFVSKYGKEFVAAASELKPDSGVNRKLVELLSVRAPSPETKLKLLKEIAEE 178

BLAST of MS004821 vs. TAIR 10
Match: AT1G51900.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 138.3 bits (347), Expect = 1.7e-32
Identity = 80/193 (41.45%), Postives = 127/193 (65.80%), Query Frame = 0

Query: 3   GLSFFDLGLSAGCSKKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVE 62
           G SF     S   + K++KQ+Q RL LLK++K   ++ LR D+++ I++   ++A  R E
Sbjct: 3   GFSFSRRRKSNAFTWKVLKQLQSRLMLLKSQKYAKSRHLRADIVDFIRSNDSKSALFRTE 62

Query: 63  QIVKDESRMVAYEILGNFCEFILQNLSYIRKHKD--CPNDVNEAVSSLMFASARCGDLPE 122
           Q++  E+ +  Y  L  F +FIL   S  +KH      +D +EAVSSL+FAS +C ++PE
Sbjct: 63  QLLLVENAITIYGFLLKFTDFILLRFSPSKKHSCLLVNDDTSEAVSSLIFASVKCREIPE 122

Query: 123 LQLIRKLFGERYGRRFETSAVELSPGNLVNRQIKEKLSANS-VSEDDKHRMINEIARDC- 182
           L +I +L G+RYG+R+ T+A+++ PGNLVN +IKEKL + S VSE DK R++ EIA++  
Sbjct: 123 LLIISELVGQRYGQRYVTTAIQVPPGNLVNTEIKEKLKSTSVVSETDKCRVMEEIAKESG 182

Query: 183 LQPQLLALEYRSD 192
            + ++L L Y+S+
Sbjct: 183 YRLEILGLGYKSE 195

BLAST of MS004821 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 136.0 bits (341), Expect = 8.5e-32
Identity = 133/498 (26.71%), Postives = 219/498 (43.98%), Query Frame = 0

Query: 4   LSFFDLGLSAGCSKKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVEQ 63
           LS F  G ++   K   K    R+KL++NK+ V+ KQ+R D+  L+++G + TA  RVE 
Sbjct: 19  LSLFRRGFNSSKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEH 78

Query: 64  IVKDESRMVAYEILGNFCEFILQNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQL 123
           ++++++   A EI+  FCE I+  L+ I K K CP D+ E ++SL+FA+ RC ++PEL  
Sbjct: 79  VIREQNIQAANEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGD 138

Query: 124 IRKLFGERYGRRFETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARDCLQPQL 183
           +R +F ++YG+ F ++A +L P   VNR + +KLS  +   + K +++ EIA+       
Sbjct: 139 LRDIFAKKYGKDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAK------- 198

Query: 184 LALEYRSDWHQKQVALIL------TIHSDERKREKALLKREKALLLHGYS-----ARSTS 243
              E++ DW   +    L      +I    +    + L   +A +           RSTS
Sbjct: 199 ---EFQVDWDTTETEQELLKPQEESIDGPRKFVSASSLPVNRAAINEPIDPTKAVPRSTS 258

Query: 244 CDTLPQFPEERIVHVDDVVELCSSTTTEGD-QILFKFKTAAAFPEEDYNGKHSSNQIHAD 303
             ++     +     +   EL            L   +  ++  E   +  HS++Q  + 
Sbjct: 259 SMSINTHYHDTESAAEAATELAKQAVAAAQVASLLATRRDSSNKEFSVSSDHSTHQKDSQ 318

Query: 304 PSD-----SWSEIENSSSKGSGKGSKRISLNKGMKKTEHNNEKGNNCHEISEQKQRKTEN 363
             D       S  ++  S+ S   +K  + N+GM +    N  G N            E+
Sbjct: 319 YMDHHHHHPGSRRQSRDSETSSYYAKPGAENRGMGRRHSYNNPGIN------------ES 378

Query: 364 WVSEGATDKEMEWASFYKKPRRRRRRKPPPLRLPSSDLKSAAYDIFTYPGYGSNTETKKL 423
              E  T+ E E      K   RRR    P  +P        +D   Y  Y   TE    
Sbjct: 379 DYEEEYTNTEAE-----AKETMRRRHSYNPRSVPPPATSEIKFDESDY--YEEETEP--- 438

Query: 424 KETRKSNAVDNGDDKNVSSYPVRNKKEALYLRAATFPPKQSPKASFTRTNSCPYKQPSHV 483
                    D      VSS P                P Q+P++  +R +S  ++    V
Sbjct: 439 ---------DEPSQGRVSSLPPNR------------APPQAPQSGESRQDSSGHQ----V 459

Query: 484 HPKLPDYDDIAAKFMALK 485
           HPKLPDYD +AA+F A++
Sbjct: 499 HPKLPDYDILAARFEAIR 459

BLAST of MS004821 vs. TAIR 10
Match: AT2G19710.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 133.7 bits (335), Expect = 4.2e-31
Identity = 70/168 (41.67%), Postives = 105/168 (62.50%), Query Frame = 0

Query: 10  GLSAGCSKKLIKQVQCRLKLLKNKKSVIAKQLREDVMELIKNGYEQTAFNRVEQIVKDES 69
           G      K  ++    RLK+LKNKK +  KQLR ++ +L+++G   TA  RVE +V++E 
Sbjct: 8   GFKPAKCKTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARIRVEHVVREEK 67

Query: 70  RMVAYEILGNFCEFILQNLSYIRKHKDCPNDVNEAVSSLMFASARCGDLPELQLIRKLFG 129
            + AYE++G +CE ++  L  I   K+CP D+ EAV+S++FAS R  D+PEL  I K F 
Sbjct: 68  TVAAYELIGIYCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVPELSEIFKQFT 127

Query: 130 ERYGRRFETSAVELSPGNLVNRQIKEKLSANSVSEDDKHRMINEIARD 178
            +YG+ F TSAVEL P + V+R + EKLSA +     K +++  IA +
Sbjct: 128 TKYGKDFSTSAVELRPDSGVSRLLVEKLSAKAPDGPTKVKILMAIAEE 175