MS004772 (gene) Bitter gourd (TR) v1

Overview
NameMS004772
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionReverse transcriptase domain-containing protein
Locationscaffold176: 184467 .. 184739 (+)
RNA-Seq ExpressionMS004772
SyntenyMS004772
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTCAATATTGGCGCCCCCCATTGAGTGTCTCTCTAAAATTAAACACTGATGCGGCATGTAGGGGTGCATCCACGAGCTTCGGCTGCATCATAAGAGACTCTTCTGATTCTCTGGTTGCCGCGACTTCAATTTGTGTTCCATTTCCGTTGTCGCCTCTTCTTGCAGAAATCAGATGCATCTTGGAGGGTCTGAAGTTTGTGGCGGCTTTCAATTTCACCCATTTAGAAGTTGAATCTGATTCTTTGTTGGCAATTCAGCTGATTCGAAATGAG

mRNA sequence

ATTCAATATTGGCGCCCCCCATTGAGTGTCTCTCTAAAATTAAACACTGATGCGGCATGTAGGGGTGCATCCACGAGCTTCGGCTGCATCATAAGAGACTCTTCTGATTCTCTGGTTGCCGCGACTTCAATTTGTGTTCCATTTCCGTTGTCGCCTCTTCTTGCAGAAATCAGATGCATCTTGGAGGGTCTGAAGTTTGTGGCGGCTTTCAATTTCACCCATTTAGAAGTTGAATCTGATTCTTTGTTGGCAATTCAGCTGATTCGAAATGAG

Coding sequence (CDS)

ATTCAATATTGGCGCCCCCCATTGAGTGTCTCTCTAAAATTAAACACTGATGCGGCATGTAGGGGTGCATCCACGAGCTTCGGCTGCATCATAAGAGACTCTTCTGATTCTCTGGTTGCCGCGACTTCAATTTGTGTTCCATTTCCGTTGTCGCCTCTTCTTGCAGAAATCAGATGCATCTTGGAGGGTCTGAAGTTTGTGGCGGCTTTCAATTTCACCCATTTAGAAGTTGAATCTGATTCTTTGTTGGCAATTCAGCTGATTCGAAATGAG

Protein sequence

IQYWRPPLSVSLKLNTDAACRGASTSFGCIIRDSSDSLVAATSICVPFPLSPLLAEIRCILEGLKFVAAFNFTHLEVESDSLLAIQLIRNE
Homology
BLAST of MS004772 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 162.9 bits (411), Expect = 1.3e-36
Identity = 84/91 (92.31%), Postives = 85/91 (93.41%), Query Frame = 0

Query: 1    IQYWRPPLSVSLKLNTDAACRGASTSFGCIIRDSSDSLVAATSICVPFPLSPLLAEIRCI 60
            +QYWRP  SVSLKLNTDAACRGASTSFGCIIRDSS SLVAATSI VPFPLSPLLAEIRCI
Sbjct: 1291 VQYWRPSSSVSLKLNTDAACRGASTSFGCIIRDSSCSLVAATSIRVPFPLSPLLAEIRCI 1350

Query: 61   LEGLKFVAAFNFTHLEVESDSLLAIQLIRNE 92
            LEGLKF AA NFTHLEVESDSLLAIQLIRNE
Sbjct: 1351 LEGLKFAAASNFTHLEVESDSLLAIQLIRNE 1381

BLAST of MS004772 vs. NCBI nr
Match: XP_022131661.1 (uncharacterized protein LOC111004786 [Momordica charantia])

HSP 1 Score: 85.9 bits (211), Expect = 2.0e-13
Identity = 44/88 (50.00%), Postives = 59/88 (67.05%), Query Frame = 0

Query: 4   WRPPLSVSLKLNTDAACRGASTSFGCIIRDSSDSLVAATSICVPFPLSPLLAEIRCILEG 63
           W PP   ++K+N+DAACR  ST  G IIRD    L+ A S+ +P PL+PL AEIR ILE 
Sbjct: 115 WLPPRDSAMKVNSDAACRSTSTGLGLIIRDHFGVLLVAKSMFLPMPLNPLFAEIRGILEA 174

Query: 64  LKFVAAFNFTHLEVESDSLLAIQLIRNE 92
           LK  A+ ++T L VESD   AI+L++ +
Sbjct: 175 LKLAASRSYTRLVVESDCQEAIRLVQGD 202

BLAST of MS004772 vs. NCBI nr
Match: XP_038722072.1 (uncharacterized protein LOC120014227 [Tripterygium wilfordii])

HSP 1 Score: 72.0 bits (175), Expect = 3.0e-09
Identity = 39/89 (43.82%), Postives = 54/89 (60.67%), Query Frame = 0

Query: 4   WRPPLSVSLKLNTDAACRGASTSFGCIIRDSSDSLVAATSICVPFPLSPLLAEIRCILEG 63
           WRPP +  +K+N DAA R +    G I RD+  + +A+ +ICVP PL+PL AE     E 
Sbjct: 128 WRPPQTSVIKINFDAALRDSKVGIGVIARDAEGTHIASKTICVPGPLNPLFAESIAAKEA 187

Query: 64  LKFVAAFNFTHLEVESDSLLAIQ-LIRNE 92
           L F  + N+  + +E DSLL I+ L RNE
Sbjct: 188 LVFAKSLNYHDVVLEGDSLLVIKALERNE 216

BLAST of MS004772 vs. NCBI nr
Match: XP_038690886.1 (uncharacterized protein LOC119989439 [Tripterygium wilfordii])

HSP 1 Score: 68.6 bits (166), Expect = 3.3e-08
Identity = 36/85 (42.35%), Postives = 49/85 (57.65%), Query Frame = 0

Query: 4   WRPPLSVSLKLNTDAACRGASTSFGCIIRDSSDSLVAATSICVPFPLSPLLAEIRCILEG 63
           WRPP    +K+N DAA R +    G I RD+  + +A+ SICVP PL+PL AE     E 
Sbjct: 65  WRPPQPSVIKINFDAALRDSKVRIGVIARDAEGAHIASKSICVPGPLNPLFAESIAAREA 124

Query: 64  LKFVAAFNFTHLEVESDSLLAIQLI 89
           L F  + N   + +E DSLL I+ +
Sbjct: 125 LVFTKSLNCHDVVLEGDSLLVIKAL 149

BLAST of MS004772 vs. NCBI nr
Match: XP_021716699.1 (uncharacterized protein LOC110684567 isoform X1 [Chenopodium quinoa] >XP_021716700.1 uncharacterized protein LOC110684567 isoform X1 [Chenopodium quinoa] >XP_021716701.1 uncharacterized protein LOC110684567 isoform X1 [Chenopodium quinoa])

HSP 1 Score: 61.2 bits (147), Expect = 5.2e-06
Identity = 33/88 (37.50%), Postives = 47/88 (53.41%), Query Frame = 0

Query: 4   WRPPLSVSLKLNTDAAC-RGASTSFGCIIRDSSDSLVAATSICVPFPLSPLLAEIRCILE 63
           W PP + S K+NTDA C RG     G ++RD   ++        P    P +AE R +LE
Sbjct: 44  WCPPAAGSFKVNTDAGCMRGLGAGLGDVLRDDQGAVCVCIVSQSPDNWEPNIAEARAVLE 103

Query: 64  GLKFVAAFNFTHLEVESDSLLAIQLIRN 91
           G+K   ++   +L VESD L  IQ +R+
Sbjct: 104 GMKLAKSYGVQYLIVESDCLAIIQALRD 131

BLAST of MS004772 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 6.2e-37
Identity = 84/91 (92.31%), Postives = 85/91 (93.41%), Query Frame = 0

Query: 1    IQYWRPPLSVSLKLNTDAACRGASTSFGCIIRDSSDSLVAATSICVPFPLSPLLAEIRCI 60
            +QYWRP  SVSLKLNTDAACRGASTSFGCIIRDSS SLVAATSI VPFPLSPLLAEIRCI
Sbjct: 1291 VQYWRPSSSVSLKLNTDAACRGASTSFGCIIRDSSCSLVAATSIRVPFPLSPLLAEIRCI 1350

Query: 61   LEGLKFVAAFNFTHLEVESDSLLAIQLIRNE 92
            LEGLKF AA NFTHLEVESDSLLAIQLIRNE
Sbjct: 1351 LEGLKFAAASNFTHLEVESDSLLAIQLIRNE 1381

BLAST of MS004772 vs. ExPASy TrEMBL
Match: A0A6J1BQ49 (uncharacterized protein LOC111004786 OS=Momordica charantia OX=3673 GN=LOC111004786 PE=4 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 9.6e-14
Identity = 44/88 (50.00%), Postives = 59/88 (67.05%), Query Frame = 0

Query: 4   WRPPLSVSLKLNTDAACRGASTSFGCIIRDSSDSLVAATSICVPFPLSPLLAEIRCILEG 63
           W PP   ++K+N+DAACR  ST  G IIRD    L+ A S+ +P PL+PL AEIR ILE 
Sbjct: 115 WLPPRDSAMKVNSDAACRSTSTGLGLIIRDHFGVLLVAKSMFLPMPLNPLFAEIRGILEA 174

Query: 64  LKFVAAFNFTHLEVESDSLLAIQLIRNE 92
           LK  A+ ++T L VESD   AI+L++ +
Sbjct: 175 LKLAASRSYTRLVVESDCQEAIRLVQGD 202

BLAST of MS004772 vs. ExPASy TrEMBL
Match: A0A803QN60 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 3.3e-06
Identity = 36/91 (39.56%), Postives = 55/91 (60.44%), Query Frame = 0

Query: 2    QYWRPPLSVSLKLNTDAAC--RGASTSFGCIIRDSSDSLVAATSICVPFPLSPLLAEIRC 61
            + W  P SV +KLN DAA   R +   FGC++R++   L+AA +   P  +SP LAEI  
Sbjct: 1013 ELWLKP-SVGIKLNVDAAISERSSKHGFGCVVRNADGILIAAFAGVKPGRVSPELAEIMG 1072

Query: 62   ILEGLKFVAAFNFTHLEVESDSLLAIQLIRN 91
            I+E L ++   ++T   VE+DSL+  + IR+
Sbjct: 1073 IMEALSWLKNHSYTQAIVETDSLVCAEAIRS 1102

BLAST of MS004772 vs. ExPASy TrEMBL
Match: A0A803QFT1 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 5.6e-06
Identity = 34/91 (37.36%), Postives = 54/91 (59.34%), Query Frame = 0

Query: 2    QYWRPPLSVSLKLNTDAACRGAST--SFGCIIRDSSDSLVAATSICVPFPLSPLLAEIRC 61
            ++W+ P   ++K+NTD A   ++T  S+ C+ RD +  LV A + C    + P +AE   
Sbjct: 1521 EHWQVPKENTIKINTDVAIFESTTRYSYSCVARDHAGRLVEAQASCRQGAVKPEIAEAVG 1580

Query: 62   ILEGLKFVAAFNFTHLEVESDSLLAIQLIRN 91
            ILE L ++    +  +EVESD L+AIQ IR+
Sbjct: 1581 ILEALSWIKQQGWRDVEVESDCLVAIQSIRS 1611

BLAST of MS004772 vs. ExPASy TrEMBL
Match: A0A6J5X9C6 (Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS28254 PE=4 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.3e-05
Identity = 35/94 (37.23%), Postives = 48/94 (51.06%), Query Frame = 0

Query: 4   WRPPLSVSLKLNTDAA------CRGASTSFGCIIRDSSDSLVAATSICVPFPLSPLLAEI 63
           WRPP     KLN D A       RGA    G I+RDS   LV A ++  P  +S L  E+
Sbjct: 438 WRPPTGNCFKLNVDGATDMETGARGA----GAIVRDSHGKLVGALAMRAPSRISVLATEL 497

Query: 64  RCILEGLKFVAAFNFTHLEVESDSLLAIQLIRNE 92
             +  G+ F    +   LE+ESDSL A+ ++ +E
Sbjct: 498 YALKVGISFALDMSLVPLEIESDSLQAVSMVNSE 527

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158377.11.3e-3692.31uncharacterized protein LOC111024874 [Momordica charantia][more]
XP_022131661.12.0e-1350.00uncharacterized protein LOC111004786 [Momordica charantia][more]
XP_038722072.13.0e-0943.82uncharacterized protein LOC120014227 [Tripterygium wilfordii][more]
XP_038690886.13.3e-0842.35uncharacterized protein LOC119989439 [Tripterygium wilfordii][more]
XP_021716699.15.2e-0637.50uncharacterized protein LOC110684567 isoform X1 [Chenopodium quinoa] >XP_0217167... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DX306.2e-3792.31uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
A0A6J1BQ499.6e-1450.00uncharacterized protein LOC111004786 OS=Momordica charantia OX=3673 GN=LOC111004... [more]
A0A803QN603.3e-0639.56Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803QFT15.6e-0637.36Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A6J5X9C61.3e-0537.23Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS28254 PE=... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 10..91
e-value: 7.0E-9
score: 37.8
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 15..91
e-value: 4.8E-10
score: 39.3
NoneNo IPR availablePANTHERPTHR47074BNAC02G40300D PROTEINcoord: 3..90
NoneNo IPR availablePANTHERPTHR47074:SF37PROTEIN, PUTATIVE-RELATEDcoord: 3..90
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 14..90
e-value: 1.40783E-15
score: 64.2576
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 12..90

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS004772.1MS004772.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity