MS004768 (gene) Bitter gourd (TR) v1

Overview


Name	MS004768
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Pathogenic type III effector avirulence factor Avr cleavage site
Location	scaffold176: 119210 .. 120311 (-)
RNA-Seq Expression	MS004768
Synteny	MS004768

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TCACAGGATTACTACTACCGCAGAAACTACATTCCCTCCTTCGGCAACTGGGACTGGAACGACCACCTTCCTTTCACCCAGTGCTTCGACTCCTCCACAACCGCCGCTTACGCCGGCGCCGCCCATGGCTACTCCGAGGTTCGCGACTTGTACGTCGCCGGCGATTTGTATCACAACGACGTCGTCACCCCCGCCATGATCGTCGTCCCTCGCTCCACGGTAACAATAACCCGCCTCTCCAGTTTGAAGTGCATTTTTTATTTTGATTTATTTTGGTTACTGAAATGATTAAATGTTAAAAAACATAAAAAAAAATTTAAATAAGAATGAGTTGTCAAATATTTAGTTCGGTGTGGAGAATTATTTTTTCTTAGTTAACTAAAATGAATTAAAGATAAAAATATAGGAATCTAAGTGAATATTTTAAAAATATAATTAAAATGAACCAAAGAGGGGCGGACACGTTGTTATTATTATTATTTTTAGTGTGGGGGGGAAGTTTTGTTGTGTTTCGTAGGAGAAAAAATTGAAGATAGGAATAAATTAATAAAACCAAAAAAGAAAGGGAAAAAGGAAATTGGAGGGGTTGCTTTAATGAGTGGATCCACATCCATCGTGTGAAGGCTTCAGCTAAGCATGCTTTCACGCAAAAAGCCTTAAAGTACTTTACTTTACCATTAATTAAAGTATATCCAAAACCAAATATATAATAAATATTTTTCTCTTTCTTTTTCTTTTTTAAATAATGAGGTTTTAATTTTATACTTTACTTCTTAATAATCTTGCCATCCATTAAATGTTTTGACAATCAAGAGAAAAGTAAAAACAAAGATATTTTAAAAAATAATAATGATGGAGCAGTAAATTTGGTTTGGAGTAGGGGTAGATGTGGAAATTTTGACTAATGAAAAAGGGGTGGGGGTGATGAATGAATTTGCAGACGAAGCGTCACAGGCAGGTGAGGGAAGGGAAGAAGGAAACAGATACAGAGACTGAAACTGCAACAGTGCAGCAAAAGGCAACGACCAAACCCAAGCCCAAGCCAGTTGACGAAGACCTCTACAAAATCTCACCTCACCTTCTCTCTGCCAAGCCCAAAAAG

mRNA sequence

TCACAGGATTACTACTACCGCAGAAACTACATTCCCTCCTTCGGCAACTGGGACTGGAACGACCACCTTCCTTTCACCCAGTGCTTCGACTCCTCCACAACCGCCGCTTACGCCGGCGCCGCCCATGGCTACTCCGAGGTTCGCGACTTGTACGTCGCCGGCGATTTGTATCACAACGACGTCGTCACCCCCGCCATGATCGTCGTCCCTCGCTCCACGACGAAGCGTCACAGGCAGGTGAGGGAAGGGAAGAAGGAAACAGATACAGAGACTGAAACTGCAACAGTGCAGCAAAAGGCAACGACCAAACCCAAGCCCAAGCCAGTTGACGAAGACCTCTACAAAATCTCACCTCACCTTCTCTCTGCCAAGCCCAAAAAG

Coding sequence (CDS)

TCACAGGATTACTACTACCGCAGAAACTACATTCCCTCCTTCGGCAACTGGGACTGGAACGACCACCTTCCTTTCACCCAGTGCTTCGACTCCTCCACAACCGCCGCTTACGCCGGCGCCGCCCATGGCTACTCCGAGGTTCGCGACTTGTACGTCGCCGGCGATTTGTATCACAACGACGTCGTCACCCCCGCCATGATCGTCGTCCCTCGCTCCACGACGAAGCGTCACAGGCAGGTGAGGGAAGGGAAGAAGGAAACAGATACAGAGACTGAAACTGCAACAGTGCAGCAAAAGGCAACGACCAAACCCAAGCCCAAGCCAGTTGACGAAGACCTCTACAAAATCTCACCTCACCTTCTCTCTGCCAAGCCCAAAAAG

Protein sequence

SQDYYYRRNYIPSFGNWDWNDHLPFTQCFDSSTTAAYAGAAHGYSEVRDLYVAGDLYHNDVVTPAMIVVPRSTTKRHRQVREGKKETDTETETATVQQKATTKPKPKPVDEDLYKISPHLLSAKPKK

Homology

[Close]

BLAST of MS004768 vs. NCBI nr
Match: XP_022156085.1 (uncharacterized protein LOC111023056 [Momordica charantia])

HSP 1 Score: 257.3 bits (656), Expect = 6.9e-65
Identity = 125/126 (99.21%), Postives = 126/126 (100.00%), Query Frame = 0


Query: 2   QDYYYRRNYIPSFGNWDWNDHLPFTQCFDSSTTAAYAGAAHGYSEVRDLYVAGDLYHNDV 61
           +DYYYRRNYIPSFGNWDWNDHLPFTQCFDSSTTAAYAGAAHGYSEVRDLYVAGDLYHNDV
Sbjct: 2   EDYYYRRNYIPSFGNWDWNDHLPFTQCFDSSTTAAYAGAAHGYSEVRDLYVAGDLYHNDV 61

Query: 62  VTPAMIVVPRSTTKRHRQVREGKKETDTETETATVQQKATTKPKPKPVDEDLYKISPHLL 121
           VTPAMIVVPRSTTKRHRQVREGKKETDTETETATVQQKATTKPKPKPVDEDLYKISPHLL
Sbjct: 62  VTPAMIVVPRSTTKRHRQVREGKKETDTETETATVQQKATTKPKPKPVDEDLYKISPHLL 121

Query: 122 SAKPKK 128
           SAKPKK
Sbjct: 122 SAKPKK 127

[Close]

BLAST of MS004768 vs. NCBI nr
Match: XP_038899118.1 (uncharacterized protein LOC120086508 [Benincasa hispida])

HSP 1 Score: 157.1 bits (396), Expect = 9.8e-35
Identity = 83/129 (64.34%), Postives = 93/129 (72.09%), Query Frame = 0


Query: 2   QDYYYRRNYIPSFGNWDWNDHLPFTQCFDSSTTAAYAGAAHGYSEVRDLYVAGDLYHNDV 61
           +DYYYRRNYIPSFGNW+WNDHLPF+ CFDSS  A       GY    DLYVAGDLY NDV
Sbjct: 2   EDYYYRRNYIPSFGNWEWNDHLPFSHCFDSSRIA-------GYPHDTDLYVAGDLYQNDV 61

Query: 62  VTPAMIVVPRSTTKRHRQVREGKKET---DTETETATVQQKATTKPKPKPVDEDLYKISP 121
           VTPAMIVVPR   KR RQ ++G+KE    +TE+    +     TKP PKPVDEDLYKISP
Sbjct: 62  VTPAMIVVPRPRRKRDRQGKQGRKENWMKETESPPNGMMNHYNTKPMPKPVDEDLYKISP 121

Query: 122 HLLSAKPKK 128
            LL+AK KK
Sbjct: 122 DLLAAKTKK 123

[Close]

BLAST of MS004768 vs. NCBI nr
Match: XP_004150335.2 (uncharacterized protein LOC101219200 [Cucumis sativus] >KGN44691.1 hypothetical protein Csa_016635 [Cucumis sativus])

HSP 1 Score: 149.1 bits (375), Expect = 2.7e-32
Identity = 79/126 (62.70%), Postives = 91/126 (72.22%), Query Frame = 0


Query: 2   QDYYYRRNYIPSFGNWDWNDHLPFTQCFDSSTTAAYAGAAHGYSEVRDLYVAGDLYHNDV 61
           +DYYYRRNYIPSFGNW+WNDHLPF+ CF+SS  A       G+    DLYVAGDLY NDV
Sbjct: 2   EDYYYRRNYIPSFGNWEWNDHLPFSHCFESSRIA-------GFPHDTDLYVAGDLYENDV 61

Query: 62  VTPAMIVVPRSTTKRHRQVREGKKETDTETETATVQQKATTKPKPKPVDEDLYKISPHLL 121
           VTPAMIVVPR   KR RQ ++ KKE    + +  +    +TKP PKPVDEDLYKISP LL
Sbjct: 62  VTPAMIVVPRPRRKRDRQGKQSKKE-KWGSPSNGMSHNYSTKPMPKPVDEDLYKISPDLL 119

Query: 122 SAKPKK 128
           +AK KK
Sbjct: 122 AAKSKK 119

[Close]

BLAST of MS004768 vs. NCBI nr
Match: OMO49336.1 (Pathogenic type III effector avirulence factor Avr cleavage site [Corchorus olitorius])

HSP 1 Score: 145.2 bits (365), Expect = 3.8e-31
Identity = 80/129 (62.02%), Postives = 96/129 (74.42%), Query Frame = 0


Query: 3   DYYYRRNYIPSFGNWDWNDHLPFTQCFDSSTTAAYAGAAHGYSEVRDLYVAGDLYHNDVV 62
           D YYRR+++P+FG+WDWN+ LPFTQCF+S+  A      + YSE RDLYVAGDLY NDVV
Sbjct: 2   DDYYRRSHVPAFGSWDWNNDLPFTQCFESARQAGL--LRYSYSEDRDLYVAGDLYENDVV 61

Query: 63  TPAMIVVPRSTTK-RHRQVREGKKETDTETETATVQQKATT---KPKPKPVDEDLYKISP 122
           TPAMIVVPR  TK R   ++EGKK++    E   V++ ATT   +P PKPVDEDLYKISP
Sbjct: 62  TPAMIVVPRRRTKVRESNLKEGKKQS---WEVGDVKEAATTPLPRPTPKPVDEDLYKISP 121

Query: 123 HLLSAKPKK 128
            LL AKPKK
Sbjct: 122 ELLYAKPKK 125

[Close]

BLAST of MS004768 vs. NCBI nr
Match: XP_021274321.1 (uncharacterized protein LOC110409336 [Herrania umbratica])

HSP 1 Score: 141.0 bits (354), Expect = 7.2e-30
Identity = 79/130 (60.77%), Postives = 94/130 (72.31%), Query Frame = 0


Query: 3   DYYYRRNYIPSFGNWDWNDHLPFTQCFDSSTTAAYAGAAHGYSEVRDLYVAGDLYHNDVV 62
           D YYRR+++P+FG+WDWN+ LPFTQCF+S+  A      + YSE RDLYVAGDLY NDVV
Sbjct: 2   DDYYRRSHVPAFGSWDWNNDLPFTQCFESARQAGL--LRYSYSEDRDLYVAGDLYENDVV 61

Query: 63  TPAMIVVPRSTTK-RHRQVREGKKETDTETETATVQQKATTKP----KPKPVDEDLYKIS 122
           TPAMIVVPR  TK R   V+EGKK+     E + V++ A+  P     PKPVDEDLYKIS
Sbjct: 62  TPAMIVVPRRRTKVRQSHVKEGKKQ---RWEVSDVKEPASPTPLPRQTPKPVDEDLYKIS 121

Query: 123 PHLLSAKPKK 128
           P LL AKPKK
Sbjct: 122 PELLYAKPKK 126

[Close]

BLAST of MS004768 vs. ExPASy TrEMBL
Match: A0A6J1DPB1 (uncharacterized protein LOC111023056 OS=Momordica charantia OX=3673 GN=LOC111023056 PE=4 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 3.4e-65
Identity = 125/126 (99.21%), Postives = 126/126 (100.00%), Query Frame = 0


Query: 2   QDYYYRRNYIPSFGNWDWNDHLPFTQCFDSSTTAAYAGAAHGYSEVRDLYVAGDLYHNDV 61
           +DYYYRRNYIPSFGNWDWNDHLPFTQCFDSSTTAAYAGAAHGYSEVRDLYVAGDLYHNDV
Sbjct: 2   EDYYYRRNYIPSFGNWDWNDHLPFTQCFDSSTTAAYAGAAHGYSEVRDLYVAGDLYHNDV 61

Query: 62  VTPAMIVVPRSTTKRHRQVREGKKETDTETETATVQQKATTKPKPKPVDEDLYKISPHLL 121
           VTPAMIVVPRSTTKRHRQVREGKKETDTETETATVQQKATTKPKPKPVDEDLYKISPHLL
Sbjct: 62  VTPAMIVVPRSTTKRHRQVREGKKETDTETETATVQQKATTKPKPKPVDEDLYKISPHLL 121

Query: 122 SAKPKK 128
           SAKPKK
Sbjct: 122 SAKPKK 127

[Close]

BLAST of MS004768 vs. ExPASy TrEMBL
Match: A0A0A0KA54 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G372860 PE=4 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 1.3e-32
Identity = 79/126 (62.70%), Postives = 91/126 (72.22%), Query Frame = 0


Query: 2   QDYYYRRNYIPSFGNWDWNDHLPFTQCFDSSTTAAYAGAAHGYSEVRDLYVAGDLYHNDV 61
           +DYYYRRNYIPSFGNW+WNDHLPF+ CF+SS  A       G+    DLYVAGDLY NDV
Sbjct: 2   EDYYYRRNYIPSFGNWEWNDHLPFSHCFESSRIA-------GFPHDTDLYVAGDLYENDV 61

Query: 62  VTPAMIVVPRSTTKRHRQVREGKKETDTETETATVQQKATTKPKPKPVDEDLYKISPHLL 121
           VTPAMIVVPR   KR RQ ++ KKE    + +  +    +TKP PKPVDEDLYKISP LL
Sbjct: 62  VTPAMIVVPRPRRKRDRQGKQSKKE-KWGSPSNGMSHNYSTKPMPKPVDEDLYKISPDLL 119

Query: 122 SAKPKK 128
           +AK KK
Sbjct: 122 AAKSKK 119

[Close]

BLAST of MS004768 vs. ExPASy TrEMBL
Match: A0A1R3FU95 (Pathogenic type III effector avirulence factor Avr cleavage site OS=Corchorus olitorius OX=93759 GN=COLO4_38590 PE=4 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 1.9e-31
Identity = 80/129 (62.02%), Postives = 96/129 (74.42%), Query Frame = 0


Query: 3   DYYYRRNYIPSFGNWDWNDHLPFTQCFDSSTTAAYAGAAHGYSEVRDLYVAGDLYHNDVV 62
           D YYRR+++P+FG+WDWN+ LPFTQCF+S+  A      + YSE RDLYVAGDLY NDVV
Sbjct: 2   DDYYRRSHVPAFGSWDWNNDLPFTQCFESARQAGL--LRYSYSEDRDLYVAGDLYENDVV 61

Query: 63  TPAMIVVPRSTTK-RHRQVREGKKETDTETETATVQQKATT---KPKPKPVDEDLYKISP 122
           TPAMIVVPR  TK R   ++EGKK++    E   V++ ATT   +P PKPVDEDLYKISP
Sbjct: 62  TPAMIVVPRRRTKVRESNLKEGKKQS---WEVGDVKEAATTPLPRPTPKPVDEDLYKISP 121

Query: 123 HLLSAKPKK 128
            LL AKPKK
Sbjct: 122 ELLYAKPKK 125

[Close]

BLAST of MS004768 vs. ExPASy TrEMBL
Match: A0A6J0ZHG7 (uncharacterized protein LOC110409336 OS=Herrania umbratica OX=108875 GN=LOC110409336 PE=4 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 3.5e-30
Identity = 79/130 (60.77%), Postives = 94/130 (72.31%), Query Frame = 0


Query: 3   DYYYRRNYIPSFGNWDWNDHLPFTQCFDSSTTAAYAGAAHGYSEVRDLYVAGDLYHNDVV 62
           D YYRR+++P+FG+WDWN+ LPFTQCF+S+  A      + YSE RDLYVAGDLY NDVV
Sbjct: 2   DDYYRRSHVPAFGSWDWNNDLPFTQCFESARQAGL--LRYSYSEDRDLYVAGDLYENDVV 61

Query: 63  TPAMIVVPRSTTK-RHRQVREGKKETDTETETATVQQKATTKP----KPKPVDEDLYKIS 122
           TPAMIVVPR  TK R   V+EGKK+     E + V++ A+  P     PKPVDEDLYKIS
Sbjct: 62  TPAMIVVPRRRTKVRQSHVKEGKKQ---RWEVSDVKEPASPTPLPRQTPKPVDEDLYKIS 121

Query: 123 PHLLSAKPKK 128
           P LL AKPKK
Sbjct: 122 PELLYAKPKK 126

[Close]

BLAST of MS004768 vs. ExPASy TrEMBL
Match: A0A061DMX9 (Uncharacterized protein OS=Theobroma cacao OX=3641 GN=TCM_002679 PE=4 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 4.6e-30
Identity = 78/130 (60.00%), Postives = 95/130 (73.08%), Query Frame = 0


Query: 3   DYYYRRNYIPSFGNWDWNDHLPFTQCFDSSTTAAYAGAAHGYSEVRDLYVAGDLYHNDVV 62
           D +YRR+++P+FG+WDWN+ LPFTQCF+S+  A      + YSE RDLYVAGDLY NDVV
Sbjct: 2   DDFYRRSHVPAFGSWDWNNDLPFTQCFESARQAGL--LRYSYSEDRDLYVAGDLYENDVV 61

Query: 63  TPAMIVVPRSTTK-RHRQVREGKKETDTETETATVQQKAT----TKPKPKPVDEDLYKIS 122
           TPAMIVVPR  TK R   V+EGKK+     E + V++ A+     +P PKPVDEDLYKIS
Sbjct: 62  TPAMIVVPRRRTKVRQSHVKEGKKQ---NWEVSDVKEPASPTPLPRPTPKPVDEDLYKIS 121

Query: 123 PHLLSAKPKK 128
           P LL AKPKK
Sbjct: 122 PELLYAKPKK 126

[Close]

BLAST of MS004768 vs. TAIR 10
Match: AT5G48500.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G10930.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 103.6 bits (257), Expect = 1.2e-22
Identity = 59/138 (42.75%), Postives = 81/138 (58.70%), Query Frame = 0


Query: 5   YYRRNYIPSFGNWDWNDHLPFTQCFDSSTTAAYAGAAH-GYSEVRDLYVAGDLYHN-DVV 64
           Y RR ++P+FG+WDWND +PFTQCF+++TT   A   +  Y + RDLY+AGDLY N  +V
Sbjct: 10  YCRRGHVPAFGSWDWNDAVPFTQCFETATTQQPAFLHYASYPQDRDLYLAGDLYDNHHLV 69

Query: 65  TPAMIVVPRSTTKRHRQVREGKKETDTETETATVQQKATTKP-------------KPKPV 124
            PA+I+VPR   K  ++  E K+    +      + +    P              PKPV
Sbjct: 70  APAVIIVPRRRAKVGQEPAEKKRNVSKQQHNYKTEAREFNAPVSCPTPVVKRRMKAPKPV 129

Query: 125 DEDLYKISPHLLSAKPKK 128
           DEDLYK+SP LLS K K+
Sbjct: 130 DEDLYKVSPQLLSVKSKR 147

The following BLAST results are available for this feature:

BLAST of MS004768 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022156085.1	6.9e-65	99.21	uncharacterized protein LOC111023056 [Momordica charantia]	[more]
XP_038899118.1	9.8e-35	64.34	uncharacterized protein LOC120086508 [Benincasa hispida]	[more]
XP_004150335.2	2.7e-32	62.70	uncharacterized protein LOC101219200 [Cucumis sativus] >KGN44691.1 hypothetical ...	[more]
OMO49336.1	3.8e-31	62.02	Pathogenic type III effector avirulence factor Avr cleavage site [Corchorus olit...	[more]
XP_021274321.1	7.2e-30	60.77	uncharacterized protein LOC110409336 [Herrania umbratica]	[more]

BLAST of MS004768 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS004768 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DPB1	3.4e-65	99.21	uncharacterized protein LOC111023056 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A0A0KA54	1.3e-32	62.70	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G372860 PE=4 SV=1	[more]
A0A1R3FU95	1.9e-31	62.02	Pathogenic type III effector avirulence factor Avr cleavage site OS=Corchorus ol...	[more]
A0A6J0ZHG7	3.5e-30	60.77	uncharacterized protein LOC110409336 OS=Herrania umbratica OX=108875 GN=LOC11040...	[more]
A0A061DMX9	4.6e-30	60.00	Uncharacterized protein OS=Theobroma cacao OX=3641 GN=TCM_002679 PE=4 SV=1	[more]

BLAST of MS004768 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G48500.1	1.2e-22	42.75	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 72..127
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 75..92
None	No IPR available	PANTHER	PTHR33699	EXPRESSED PROTEIN	coord: 5..127
None	No IPR available	PANTHER	PTHR33699:SF2	PATHOGENIC TYPE III EFFECTOR AVIRULENCE FACTOR AVR AVRRPT-CLEAVAGE: CLEAVAGE SITE PROTEIN-RELATED	coord: 5..127

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS004768.1	MS004768.1	mRNA