MS004614 (gene) Bitter gourd (TR) v1

BLAST of MS004614 vs. NCBI nr
Match: XP_022135277.1 (protein PAT1 homolog 1 isoform X1 [Momordica charantia])

HSP 1 Score: 1533.5 bits (3969), Expect = 0.0e+00
Identity = 767/773 (99.22%), Postives = 769/773 (99.48%), Query Frame = 0

Query: 1   NAVEDARFDASQYAFFGKDVMEEVELGGLEDEEDDALAGGIDEEEFLFDKESEDFRPTSD 60
           N+ EDARFDASQYAFFGKDVMEEVELGGLEDEEDDALAGGIDEEEFLFDKESEDFRPTSD
Sbjct: 26  NSAEDARFDASQYAFFGKDVMEEVELGGLEDEEDDALAGGIDEEEFLFDKESEDFRPTSD 85

Query: 61  IDDLVTSFEKFNNVGGGPRGVIGGRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQEGK 120
           IDDLVTSFEKFNNVGGGPRGVIGGRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQEGK
Sbjct: 86  IDDLVTSFEKFNNVGGGPRGVIGGRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQEGK 145

Query: 121 RWSSHPHSSSLAESTSLYRTSSYPDQPQPQQYHQQFSSDPILVPKSPYPPTGMSPQASPN 180
           RWSSHPHSSSLAESTSLYRTSSYPDQPQPQQYHQQFSSDPILVPKSPYPPTGMSPQASPN
Sbjct: 146 RWSSHPHSSSLAESTSLYRTSSYPDQPQPQQYHQQFSSDPILVPKSPYPPTGMSPQASPN 205

Query: 181 HVNMPFVPGGRHVASLSPSNLTPPNSQIGGFNPGARFGGNMPQLSAGLSINSGPPSQWNN 240
           HVNMPFVPGG HVASLSPSNLTPPN QIGGFNPGARFGGNMPQLSAGLSINSGPPSQWNN
Sbjct: 206 HVNMPFVPGGHHVASLSPSNLTPPNLQIGGFNPGARFGGNMPQLSAGLSINSGPPSQWNN 265

Query: 241 QTGMFPGEHSSHLNNLLPRQLSNQNGFPQLLPQSQQQQQHRLQQPPFGGSLPGFQSHLFN 300
           QTGMFPGEHSSHLNNLLPRQLSNQNGFPQLLPQSQQQQQHRLQQPPFGGSLPGFQSHLFN
Sbjct: 266 QTGMFPGEHSSHLNNLLPRQLSNQNGFPQLLPQSQQQQQHRLQQPPFGGSLPGFQSHLFN 325

Query: 301 SHISSGPPPLMNKLEAMLGGLPDMRDQRPKYVQKGRQNARFIHQGHENNSFKNDFGWPFY 360
           SHISSGPPPLMNKLEAMLGGLPDMRDQRPK VQKGRQNARFIHQGHENNSFKNDFGWPFY
Sbjct: 326 SHISSGPPPLMNKLEAMLGGLPDMRDQRPKSVQKGRQNARFIHQGHENNSFKNDFGWPFY 385

Query: 361 RSKYMTTDELENIVRMQLAATHSNDPYVDDYYHQACLARKSAGAKLRHHFCPNQLRDLPP 420
           RSKYMTTDELENIVRMQLAATHSNDPYVDDYYHQACLARKSAGAKLRHHFCPNQLRDLPP
Sbjct: 386 RSKYMTTDELENIVRMQLAATHSNDPYVDDYYHQACLARKSAGAKLRHHFCPNQLRDLPP 445

Query: 421 RARANNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSDAGSTEQKIPEKPLEQEPML 480
           RARANNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSDAG+TEQKIPEKPLEQEPML
Sbjct: 446 RARANNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSDAGTTEQKIPEKPLEQEPML 505

Query: 481 AARVTIEDGHCLLLDVDDIDRFLQFNQFQDSGALRRRRQVLLEGLAASFHIVDPLGKDGR 540
           AARVTIEDGHCLLLDVDDIDRFLQFNQFQDSGALRRRRQVLLEGLAASFHIVDPLGKDGR
Sbjct: 506 AARVTIEDGHCLLLDVDDIDRFLQFNQFQDSGALRRRRQVLLEGLAASFHIVDPLGKDGR 565

Query: 541 AVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRIVCMAIFRHLRFLFGSLPSD 600
           AVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRIVCMAIFRHLRFLFGSLPSD
Sbjct: 566 AVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRIVCMAIFRHLRFLFGSLPSD 625

Query: 601 PTTADSVNDLARIVSLRTHGMDLGALSACLAAVVCSSEQPPLRPLGNPAGDGASLILKSV 660
           PTTADSVNDLARIVSLRTHGMDLGALSACLAAVVCSSEQPPLRPLGNPAGDGASLILKSV
Sbjct: 626 PTTADSVNDLARIVSLRTHGMDLGALSACLAAVVCSSEQPPLRPLGNPAGDGASLILKSV 685

Query: 661 LERATELLTDPHAASNYNITHRALWQASFDEFFGLLTKYCVNKYDSIMQSLLRQSPQNAA 720
           LERATELLTDPHAASNYNITHRALWQASFDEFFGLLTKYCVNKYDSIMQSLLRQSPQNAA
Sbjct: 686 LERATELLTDPHAASNYNITHRALWQASFDEFFGLLTKYCVNKYDSIMQSLLRQSPQNAA 745

Query: 721 AAVSDAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSMSVGGRNNFESV 774
           AAVSDAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSMSVGGRNNFESV
Sbjct: 746 AAVSDAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSMSVGGRNNFESV 798

BLAST of MS004614 vs. NCBI nr
Match: XP_022135286.1 (protein PAT1 homolog 1 isoform X2 [Momordica charantia])

HSP 1 Score: 1528.8 bits (3957), Expect = 0.0e+00
Identity = 765/769 (99.48%), Postives = 766/769 (99.61%), Query Frame = 0

Query: 5   DARFDASQYAFFGKDVMEEVELGGLEDEEDDALAGGIDEEEFLFDKESEDFRPTSDIDDL 64
           DARFDASQYAFFGKDVMEEVELGGLEDEEDDALAGGIDEEEFLFDKESEDFRPTSDIDDL
Sbjct: 29  DARFDASQYAFFGKDVMEEVELGGLEDEEDDALAGGIDEEEFLFDKESEDFRPTSDIDDL 88

Query: 65  VTSFEKFNNVGGGPRGVIGGRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQEGKRWSS 124
           VTSFEKFNNVGGGPRGVIGGRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQEGKRWSS
Sbjct: 89  VTSFEKFNNVGGGPRGVIGGRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQEGKRWSS 148

Query: 125 HPHSSSLAESTSLYRTSSYPDQPQPQQYHQQFSSDPILVPKSPYPPTGMSPQASPNHVNM 184
           HPHSSSLAESTSLYRTSSYPDQPQPQQYHQQFSSDPILVPKSPYPPTGMSPQASPNHVNM
Sbjct: 149 HPHSSSLAESTSLYRTSSYPDQPQPQQYHQQFSSDPILVPKSPYPPTGMSPQASPNHVNM 208

Query: 185 PFVPGGRHVASLSPSNLTPPNSQIGGFNPGARFGGNMPQLSAGLSINSGPPSQWNNQTGM 244
           PFVPGG HVASLSPSNLTPPN QIGGFNPGARFGGNMPQLSAGLSINSGPPSQWNNQTGM
Sbjct: 209 PFVPGGHHVASLSPSNLTPPNLQIGGFNPGARFGGNMPQLSAGLSINSGPPSQWNNQTGM 268

Query: 245 FPGEHSSHLNNLLPRQLSNQNGFPQLLPQSQQQQQHRLQQPPFGGSLPGFQSHLFNSHIS 304
           FPGEHSSHLNNLLPRQLSNQNGFPQLLPQSQQQQQHRLQQPPFGGSLPGFQSHLFNSHIS
Sbjct: 269 FPGEHSSHLNNLLPRQLSNQNGFPQLLPQSQQQQQHRLQQPPFGGSLPGFQSHLFNSHIS 328

Query: 305 SGPPPLMNKLEAMLGGLPDMRDQRPKYVQKGRQNARFIHQGHENNSFKNDFGWPFYRSKY 364
           SGPPPLMNKLEAMLGGLPDMRDQRPK VQKGRQNARFIHQGHENNSFKNDFGWPFYRSKY
Sbjct: 329 SGPPPLMNKLEAMLGGLPDMRDQRPKSVQKGRQNARFIHQGHENNSFKNDFGWPFYRSKY 388

Query: 365 MTTDELENIVRMQLAATHSNDPYVDDYYHQACLARKSAGAKLRHHFCPNQLRDLPPRARA 424
           MTTDELENIVRMQLAATHSNDPYVDDYYHQACLARKSAGAKLRHHFCPNQLRDLPPRARA
Sbjct: 389 MTTDELENIVRMQLAATHSNDPYVDDYYHQACLARKSAGAKLRHHFCPNQLRDLPPRARA 448

Query: 425 NNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSDAGSTEQKIPEKPLEQEPMLAARV 484
           NNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSDAG+TEQKIPEKPLEQEPMLAARV
Sbjct: 449 NNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSDAGTTEQKIPEKPLEQEPMLAARV 508

Query: 485 TIEDGHCLLLDVDDIDRFLQFNQFQDSGALRRRRQVLLEGLAASFHIVDPLGKDGRAVGL 544
           TIEDGHCLLLDVDDIDRFLQFNQFQDSGALRRRRQVLLEGLAASFHIVDPLGKDGRAVGL
Sbjct: 509 TIEDGHCLLLDVDDIDRFLQFNQFQDSGALRRRRQVLLEGLAASFHIVDPLGKDGRAVGL 568

Query: 545 APKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRIVCMAIFRHLRFLFGSLPSDPTTA 604
           APKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRIVCMAIFRHLRFLFGSLPSDPTTA
Sbjct: 569 APKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRIVCMAIFRHLRFLFGSLPSDPTTA 628

Query: 605 DSVNDLARIVSLRTHGMDLGALSACLAAVVCSSEQPPLRPLGNPAGDGASLILKSVLERA 664
           DSVNDLARIVSLRTHGMDLGALSACLAAVVCSSEQPPLRPLGNPAGDGASLILKSVLERA
Sbjct: 629 DSVNDLARIVSLRTHGMDLGALSACLAAVVCSSEQPPLRPLGNPAGDGASLILKSVLERA 688

Query: 665 TELLTDPHAASNYNITHRALWQASFDEFFGLLTKYCVNKYDSIMQSLLRQSPQNAAAAVS 724
           TELLTDPHAASNYNITHRALWQASFDEFFGLLTKYCVNKYDSIMQSLLRQSPQNAAAAVS
Sbjct: 689 TELLTDPHAASNYNITHRALWQASFDEFFGLLTKYCVNKYDSIMQSLLRQSPQNAAAAVS 748

Query: 725 DAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSMSVGGRNNFESV 774
           DAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSMSVGGRNNFESV
Sbjct: 749 DAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSMSVGGRNNFESV 797

BLAST of MS004614 vs. NCBI nr
Match: XP_022135295.1 (protein PAT1 homolog 1 isoform X3 [Momordica charantia])

HSP 1 Score: 1471.1 bits (3807), Expect = 0.0e+00
Identity = 743/773 (96.12%), Postives = 745/773 (96.38%), Query Frame = 0

Query: 1   NAVEDARFDASQYAFFGKDVMEEVELGGLEDEEDDALAGGIDEEEFLFDKESEDFRPTSD 60
           N+ EDARFDASQYAFFGKDVMEEVELGGLEDEEDDALAGGIDEEEFLFDKESEDFRPTSD
Sbjct: 26  NSAEDARFDASQYAFFGKDVMEEVELGGLEDEEDDALAGGIDEEEFLFDKESEDFRPTSD 85

Query: 61  IDDLVTSFEKFNNVGGGPRGVIGGRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQEGK 120
           IDDLVTSFEKFNNVGGGPRGVIGGRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQEGK
Sbjct: 86  IDDLVTSFEKFNNVGGGPRGVIGGRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQEGK 145

Query: 121 RWSSHPHSSSLAESTSLYRTSSYPDQPQPQQYHQQFSSDPILVPKSPYPPTGMSPQASPN 180
           RWSSHPHSSSLAESTSLYRTSSYPDQPQPQQYHQQFSSDPILVPKSPYPPTGMSPQASPN
Sbjct: 146 RWSSHPHSSSLAESTSLYRTSSYPDQPQPQQYHQQFSSDPILVPKSPYPPTGMSPQASPN 205

Query: 181 HVNMPFVPGGRHVASLSPSNLTPPNSQIGGFNPGARFGGNMPQLSAGLSINSGPPSQWNN 240
           H                          IGGFNPGARFGGNMPQLSAGLSINSGPPSQWNN
Sbjct: 206 H--------------------------IGGFNPGARFGGNMPQLSAGLSINSGPPSQWNN 265

Query: 241 QTGMFPGEHSSHLNNLLPRQLSNQNGFPQLLPQSQQQQQHRLQQPPFGGSLPGFQSHLFN 300
           QTGMFPGEHSSHLNNLLPRQLSNQNGFPQLLPQSQQQQQHRLQQPPFGGSLPGFQSHLFN
Sbjct: 266 QTGMFPGEHSSHLNNLLPRQLSNQNGFPQLLPQSQQQQQHRLQQPPFGGSLPGFQSHLFN 325

Query: 301 SHISSGPPPLMNKLEAMLGGLPDMRDQRPKYVQKGRQNARFIHQGHENNSFKNDFGWPFY 360
           SHISSGPPPLMNKLEAMLGGLPDMRDQRPK VQKGRQNARFIHQGHENNSFKNDFGWPFY
Sbjct: 326 SHISSGPPPLMNKLEAMLGGLPDMRDQRPKSVQKGRQNARFIHQGHENNSFKNDFGWPFY 385

Query: 361 RSKYMTTDELENIVRMQLAATHSNDPYVDDYYHQACLARKSAGAKLRHHFCPNQLRDLPP 420
           RSKYMTTDELENIVRMQLAATHSNDPYVDDYYHQACLARKSAGAKLRHHFCPNQLRDLPP
Sbjct: 386 RSKYMTTDELENIVRMQLAATHSNDPYVDDYYHQACLARKSAGAKLRHHFCPNQLRDLPP 445

Query: 421 RARANNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSDAGSTEQKIPEKPLEQEPML 480
           RARANNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSDAG+TEQKIPEKPLEQEPML
Sbjct: 446 RARANNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSDAGTTEQKIPEKPLEQEPML 505

Query: 481 AARVTIEDGHCLLLDVDDIDRFLQFNQFQDSGALRRRRQVLLEGLAASFHIVDPLGKDGR 540
           AARVTIEDGHCLLLDVDDIDRFLQFNQFQDSGALRRRRQVLLEGLAASFHIVDPLGKDGR
Sbjct: 506 AARVTIEDGHCLLLDVDDIDRFLQFNQFQDSGALRRRRQVLLEGLAASFHIVDPLGKDGR 565

Query: 541 AVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRIVCMAIFRHLRFLFGSLPSD 600
           AVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRIVCMAIFRHLRFLFGSLPSD
Sbjct: 566 AVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRIVCMAIFRHLRFLFGSLPSD 625

Query: 601 PTTADSVNDLARIVSLRTHGMDLGALSACLAAVVCSSEQPPLRPLGNPAGDGASLILKSV 660
           PTTADSVNDLARIVSLRTHGMDLGALSACLAAVVCSSEQPPLRPLGNPAGDGASLILKSV
Sbjct: 626 PTTADSVNDLARIVSLRTHGMDLGALSACLAAVVCSSEQPPLRPLGNPAGDGASLILKSV 685

Query: 661 LERATELLTDPHAASNYNITHRALWQASFDEFFGLLTKYCVNKYDSIMQSLLRQSPQNAA 720
           LERATELLTDPHAASNYNITHRALWQASFDEFFGLLTKYCVNKYDSIMQSLLRQSPQNAA
Sbjct: 686 LERATELLTDPHAASNYNITHRALWQASFDEFFGLLTKYCVNKYDSIMQSLLRQSPQNAA 745

Query: 721 AAVSDAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSMSVGGRNNFESV 774
           AAVSDAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSMSVGGRNNFESV
Sbjct: 746 AAVSDAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSMSVGGRNNFESV 772

BLAST of MS004614 vs. NCBI nr
Match: XP_038899006.1 (protein PAT1 homolog isoform X1 [Benincasa hispida])

HSP 1 Score: 1347.4 bits (3486), Expect = 0.0e+00
Identity = 691/785 (88.03%), Postives = 722/785 (91.97%), Query Frame = 0

Query: 1   NAVEDARFDASQYAFFGKDVMEEVELGGLEDEEDDALAGGIDEEEFLFDKESEDFRPTSD 60
           N+ EDA FDASQYAFFGKDVMEEVELGGLEDEEDDALA GI+EEEFLFDKESEDFRP SD
Sbjct: 25  NSTEDALFDASQYAFFGKDVMEEVELGGLEDEEDDALAAGIEEEEFLFDKESEDFRPPSD 84

Query: 61  IDDLVTSFEKFNNVGGGPRGVIGGRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQEGK 120
           IDDLV+SFEK N VG GPRGVIGGR+LRESS VNEWAREEGFSNWLAQQ Y+ ESAQEGK
Sbjct: 85  IDDLVSSFEKLNEVGSGPRGVIGGRILRESSLVNEWAREEGFSNWLAQQGYNVESAQEGK 144

Query: 121 RWSSHPHSSSLAESTSLYRTSSYPDQ--PQPQQYHQQFSSDPILVPKSPYPPTGMSPQAS 180
           RWSSHPHSSS+AESTSLYRTSSYPDQ  PQPQQYHQQFSS+PILVPKS YPP+G+SP AS
Sbjct: 145 RWSSHPHSSSIAESTSLYRTSSYPDQPPPQPQQYHQQFSSEPILVPKSSYPPSGISPHAS 204

Query: 181 PN----HVNMPFVPGGRHVASLSPSNLTPPNSQIGGFNPGARFGGNMPQLSAGLSINSGP 240
           PN    H+NMPFVPGGRHV SLSPSNLTPPNSQI GFNPG+RF GN+PQL++GLSIN GP
Sbjct: 205 PNQHSSHLNMPFVPGGRHVVSLSPSNLTPPNSQIAGFNPGSRF-GNIPQLNSGLSINGGP 264

Query: 241 PSQWNNQTGMFPGEHSSHLNNLLPRQLSNQNGFPQLLP---------QSQQQQQHRLQ-- 300
            SQW +QTGMFPGE SS+LNNLLP QLS QNGFPQL P         Q QQQQQHRLQ  
Sbjct: 265 QSQWVSQTGMFPGEQSSNLNNLLPHQLSYQNGFPQLPPPQQQQQQQQQQQQQQQHRLQNP 324

Query: 301 -QPPFGGSLPGFQSHLFNSHISSGPPPLMNKLEAMLGGLPDMRDQRPKYVQKGRQNARFI 360
            QPPFGGSLPGFQSHLFNSH+SSGPP LMNKLEAMLGGLPDMRDQRP+  QK RQN RFI
Sbjct: 325 IQPPFGGSLPGFQSHLFNSHLSSGPPQLMNKLEAMLGGLPDMRDQRPR-SQKSRQNTRFI 384

Query: 361 HQGHENNSFKNDFGWPFYRSKYMTTDELENIVRMQLAATHSNDPYVDDYYHQACLARKSA 420
            QG+E NS +NDFGWPFYRSKYMT DELENIVRMQLAATHSNDPYVDDYYHQACL+RKSA
Sbjct: 385 QQGYETNSVRNDFGWPFYRSKYMTADELENIVRMQLAATHSNDPYVDDYYHQACLSRKSA 444

Query: 421 GAKLRHHFCPNQLRDLPPRARANNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSDA 480
           GAKLRHHFCPNQLRDLPPRARANNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSS  
Sbjct: 445 GAKLRHHFCPNQLRDLPPRARANNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSVG 504

Query: 481 GSTEQKIPEKPLEQEPMLAARVTIEDGHCLLLDVDDIDRFLQFNQFQDSGA-LRRRRQVL 540
           GST+QK+ EKPLEQEPMLAARVTIEDGHCLLLDVDDIDRFLQFNQFQD GA LRRRRQVL
Sbjct: 505 GSTDQKVSEKPLEQEPMLAARVTIEDGHCLLLDVDDIDRFLQFNQFQDGGAQLRRRRQVL 564

Query: 541 LEGLAASFHIVDPLGKDGRAVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRI 600
           LEGLA+SFHIVDPL KDG AVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLL+PGGELMRI
Sbjct: 565 LEGLASSFHIVDPLSKDGNAVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLMPGGELMRI 624

Query: 601 VCMAIFRHLRFLFGSLPSDPTTADSVNDLARIVSLRTHGMDLGALSACLAAVVCSSEQPP 660
           VCMAIFRHLRFLFGS+ SDP  ADSV+DLARIVSLRTH MDLGALSACLAAVVCSSEQPP
Sbjct: 625 VCMAIFRHLRFLFGSVSSDPAIADSVSDLARIVSLRTHSMDLGALSACLAAVVCSSEQPP 684

Query: 661 LRPLGNPAGDGASLILKSVLERATELLTDPHAASNYNITHRALWQASFDEFFGLLTKYCV 720
           LRPLG+PAGDGASLILKSVLERAT LLTDPHAASNYNITHRALWQASFD+FFGLLTKYCV
Sbjct: 685 LRPLGSPAGDGASLILKSVLERATVLLTDPHAASNYNITHRALWQASFDDFFGLLTKYCV 744

Query: 721 NKYDSIMQSLLRQSPQNAAAAVSDAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRS 767
           NKYD+IM+SLLRQSPQNAAAAVSDAA AISQEMPVEVLRASLPHTDEHQRKVLIDFAQRS
Sbjct: 745 NKYDTIMRSLLRQSPQNAAAAVSDAATAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRS 804

BLAST of MS004614 vs. NCBI nr
Match: XP_023535657.1 (protein PAT1 homolog 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1295.8 bits (3352), Expect = 0.0e+00
Identity = 671/784 (85.59%), Postives = 705/784 (89.92%), Query Frame = 0

Query: 1   NAVEDARFDASQYAFFGKDVMEEVELGGLEDEEDDALAGGI--DEEEFLFDKESEDFRPT 60
           N+ EDA FDASQYAFFGKDVMEEVELGGLEDEEDD LA GI  +EEEFLFDKESEDFRP 
Sbjct: 25  NSTEDALFDASQYAFFGKDVMEEVELGGLEDEEDDTLAAGIEEEEEEFLFDKESEDFRPP 84

Query: 61  SDIDDLVTSFEKFNNVGGGPRGVIGGRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQE 120
           SDIDDLV+SFE+ + VG GP GVIGGR LRESSSVNEWAREEGFSNWLAQQ Y+ +SAQE
Sbjct: 85  SDIDDLVSSFERLSEVGSGPTGVIGGRALRESSSVNEWAREEGFSNWLAQQGYNVQSAQE 144

Query: 121 GKRWSSHPHSSSLAESTSLYRTSSYPD-QPQPQQYHQQFSSDPILVPKSPYPPTGMSPQA 180
           GKRWSSHPH SSLAESTSLYRTSSY D QPQPQQYHQQFSS+PI VPKS YPP+G+SP A
Sbjct: 145 GKRWSSHPHFSSLAESTSLYRTSSYADQQPQPQQYHQQFSSEPISVPKSSYPPSGISPHA 204

Query: 181 SPN----HVNMPFVPGGRHVASLSPSNLTPPNSQIGGFNPGARFGGNMPQLSAGLSINSG 240
           SPN    H+NMPFVP GRHV SLSPSNLTPPNSQI GF  G+RF GNMPQL++GLS N G
Sbjct: 205 SPNQHSSHLNMPFVPSGRHVVSLSPSNLTPPNSQIAGFISGSRF-GNMPQLNSGLSANGG 264

Query: 241 PPSQWNNQTGMFPGEHSSHLNNLLPRQLSNQNGFPQLLP----QSQQQQQHRLQ---QPP 300
           P SQW NQ GMF GEHSSHLNNLLP+QL NQNGFPQL P    Q QQQQQHRLQ   QPP
Sbjct: 265 PQSQWVNQIGMFRGEHSSHLNNLLPQQLPNQNGFPQLPPQPPQQQQQQQQHRLQHPVQPP 324

Query: 301 FGGSLPGFQSHLFNSHISSGPPPLMNKLEAMLGGLPDMRDQRPKYVQKGRQNARFIHQGH 360
           FGGSLPGFQSHLFNSH+SSGPP LMNKLEAML G+PDMRDQRP+  QKGRQN RFIHQG+
Sbjct: 325 FGGSLPGFQSHLFNSHVSSGPPHLMNKLEAML-GVPDMRDQRPR-SQKGRQNPRFIHQGN 384

Query: 361 ENNSFKNDFGWPFYRSKYMTTDELENIVRMQLAATHSNDPYVDDYYHQACLARKSAGAKL 420
           E +SF+N+FGWPF RSKYM  DELENIVRMQLAATHSNDPYVDDYYHQACL+RKSAGAKL
Sbjct: 385 ETSSFRNNFGWPFCRSKYMGADELENIVRMQLAATHSNDPYVDDYYHQACLSRKSAGAKL 444

Query: 421 RHHFCPNQLRDLPPRARANNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSDAGSTE 480
           RHHFCPNQLRDLPP ARANNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSS  GS++
Sbjct: 445 RHHFCPNQLRDLPPHARANNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSVGGSSD 504

Query: 481 QKIPEKPLEQEPMLAARVTIEDGHCLLLDVDDIDRFLQFNQFQDSGA-LRRRRQVLLEGL 540
           QK+ EKPLEQEPMLAARVTIEDGHCLLLDVDDIDR LQFNQFQD GA LRRRRQVLLEGL
Sbjct: 505 QKVSEKPLEQEPMLAARVTIEDGHCLLLDVDDIDRLLQFNQFQDGGAQLRRRRQVLLEGL 564

Query: 541 AASFHIVDPLGKDGRAVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRIVCMA 600
           AAS HIVDP  KDG  VGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGEL RIVCMA
Sbjct: 565 AASLHIVDPCSKDGHTVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELKRIVCMA 624

Query: 601 IFRHLRFLFGSLPSDPTTADSVNDLARIVSLRTHGMDLGALSACLAAVVCSSEQPPLRPL 660
           IFRHLRFLFGS+PSDP  ADSV++LARIVSL+T  MDLGALSACLAAVVCSSEQPPLRPL
Sbjct: 625 IFRHLRFLFGSVPSDPGAADSVSELARIVSLQTQSMDLGALSACLAAVVCSSEQPPLRPL 684

Query: 661 GNPAGDGASLILKSVLERATELLTDPHAASNYNITHRALWQASFDEFFGLLTKYCVNKYD 720
           G+PAGDGASLILKSVLERATELLT PHAASNYNITHR+LWQASFDEFFGLLTKYCVNKYD
Sbjct: 685 GSPAGDGASLILKSVLERATELLTAPHAASNYNITHRSLWQASFDEFFGLLTKYCVNKYD 744

Query: 721 SIMQSLLRQSPQNAAAAVSDAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSMSVG 770
           SIMQSLLRQSPQN A AV D A AISQEMPVEVLRASLPHTDEHQ++VLIDFAQRSMSVG
Sbjct: 745 SIMQSLLRQSPQNPAVAVLDQATAISQEMPVEVLRASLPHTDEHQKRVLIDFAQRSMSVG 804

BLAST of MS004614 vs. ExPASy Swiss-Prot
Match: Q0WPK4 (Protein PAT1 homolog OS=Arabidopsis thaliana OX=3702 GN=PAT1 PE=1 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 5.3e-203
Identity = 415/779 (53.27%), Postives = 523/779 (67.14%), Query Frame = 0

Query: 1   NAVEDARFDASQYAFFGKDVMEEVELGGLEDEEDDALAGGIDEEEFLFDKES-EDFRPTS 60
           N+  +  FDASQYAFFG DV+EEVELGGLE+E++     GI  E+F FDKE   D R  S
Sbjct: 25  NSTGNTMFDASQYAFFGNDVVEEVELGGLEEEDEILSFTGI-AEDFSFDKEEVGDSRLLS 84

Query: 61  DIDDLVTSFEKFNNVGG--GPRGVIGGRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQ 120
           D+DDL ++F K N         G I  R   ++S   EW   E   NW  +Q  D ++ +
Sbjct: 85  DVDDLASTFSKLNREPDVYSNTGPITDRRSSQNSLAAEWTHGEELPNWYGRQILDSDAIK 144

Query: 121 EGKRWSSHPHSS-SLAESTSLYRTSSYPD-QPQPQQYH--QQFSSDPILVPKSPY----P 180
           + K WS+ P SS    E     RT  YP+ Q Q  Q H  QQFSS+PILVPKS +    P
Sbjct: 145 DDKVWSAQPFSSLDRVEQRIPDRTKLYPEPQRQLHQDHNQQQFSSEPILVPKSSFVSYPP 204

Query: 181 PTGMSPQASPNHVNMPFVPGGRHVASLSPSNLTPPNSQIGGFNPGA-RFGGNMPQLSAGL 240
           P  +SP     H N+P+  GG  + S + S       Q+   + G+ +  GN PQ    L
Sbjct: 205 PGSISPDQRLGHPNIPYQSGGPQMGSPNFSPFPNLQPQLPSMHHGSPQHTGNRPQFRPAL 264

Query: 241 SINSGPPSQWNNQTGMFPGEHSSHLNNLLPRQLSNQNGFPQLLPQSQQQQQHRL---QQP 300
            +N+ PP+QW N+  M PG+ S  +NN + +Q  +QNG   L+P   Q  Q+RL    QP
Sbjct: 265 PLNNLPPAQWMNRQNMHPGDSSGIMNNAMLQQPPHQNG---LMPPQMQGSQNRLPHPMQP 324

Query: 301 PFGGSLPGFQSHLFNSHISSGPPPLMNKLEAMLGGLPDMRDQRPKYVQKGRQNARFIHQG 360
           P  G +PG Q  LFNSH+S          + ML G  D+R+ RP      RQN RF  QG
Sbjct: 325 PL-GHMPGMQPQLFNSHLSRSSS--SGNYDGML-GFGDLREVRPGSGHGNRQNVRFPQQG 384

Query: 361 HENNSFKNDFGWPFYRSKYMTTDELENIVRMQLAATHSNDPYVDDYYHQACLARKSAGAK 420
            +    +    +PF RSKYM+  E+ENI+RMQL ATHSNDPYVDDYYHQACLA+KSAGAK
Sbjct: 385 FDAGVQRR---YPF-RSKYMSAGEIENILRMQLVATHSNDPYVDDYYHQACLAKKSAGAK 444

Query: 421 LRHHFCPNQLRDLPPRARANNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSDAGST 480
           L+HHFCPN LRDL  RAR+NNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPP+S+  G+ 
Sbjct: 445 LKHHFCPNHLRDLQQRARSNNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPNSAKFGNA 504

Query: 481 EQKIPEKPLEQEPMLAARVTIEDGHCLLLDVDDIDRFLQFNQFQDSG-ALRRRRQVLLEG 540
           E K  +KPL+QEPMLAARV IEDG CLLL+VDDIDRFL+FNQ QD G  L++RRQ LL+ 
Sbjct: 505 EHKPTDKPLDQEPMLAARVYIEDGLCLLLEVDDIDRFLEFNQLQDGGHQLKQRRQALLQS 564

Query: 541 LAASFHIVDPLGKDGRAVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRIVCM 600
           LA S  + DPL K+G++  L   DDF+FLR++SLPKGRKLL +YLQL+ PG +LMRIVCM
Sbjct: 565 LAVSLQLGDPLAKNGQSQSL---DDFLFLRVISLPKGRKLLIRYLQLIFPGSDLMRIVCM 624

Query: 601 AIFRHLRFLFGSLPSDPTTADSVNDLARIVSLRTHGMDLGALSACLAAVVCSSEQPPLRP 660
           AIFRHLR LFG L SDP    + N LA +++L    M+LG +S CLAAV CSSEQ PLRP
Sbjct: 625 AIFRHLRSLFGVLSSDPDIIKTTNKLATVINLCIQNMELGPVSTCLAAVSCSSEQAPLRP 684

Query: 661 LGNPAGDGASLILKSVLERATELLTDPHAASNYNITHRALWQASFDEFFGLLTKYCVNKY 720
           LG+P GDGAS +LKS+L+RA+EL+     A+N+N    ALW+ASF+EFF +L +YC++KY
Sbjct: 685 LGSPVGDGASTVLKSILDRASELI----RANNFNNAGIALWRASFNEFFNMLMRYCISKY 744

Query: 721 DSIMQSLLRQSPQNAAAAVS-DAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSM 763
           DSIMQSL  Q P + A  +S +AA AI +EMP+E+LR+S PH DE Q+++L++F +RSM
Sbjct: 745 DSIMQSL--QLPPHFATEISEEAAKAIVREMPIELLRSSFPHIDEQQKRILMEFLKRSM 782

BLAST of MS004614 vs. ExPASy Swiss-Prot
Match: F4J077 (Protein PAT1 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=PAT1H1 PE=1 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 7.5e-165
Identity = 365/792 (46.09%), Postives = 499/792 (63.01%), Query Frame = 0

Query: 8   FDASQYAFFGKDVMEEVELGGLEDEEDDA-LAGGIDEEEF-LFDK-ESEDFRPTSDIDDL 67
           FDASQY FFG++ ++++ELGGL+D+   A + G  D++E+ LFDK E       SD+DDL
Sbjct: 25  FDASQYEFFGQN-LDDMELGGLDDDGVIAPVLGHADDDEYHLFDKGEGAGLGSLSDMDDL 84

Query: 68  VTSFEKFNNVGGGPR--GVIG----GRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQE 127
            T+F K N V  GP+  GVIG    G   RESSS  +W ++   ++WL      DE  QE
Sbjct: 85  ATTFAKLNRVVTGPKHPGVIGDRGSGSFSRESSSATDWTQDAELTSWL------DEQDQE 144

Query: 128 GKRWSSHPHSSSLAESTSLYRTSSYPDQPQPQQYHQQFSSDPILVPKSPY----PPTGMS 187
            KRWSS P   S A S  LYRTSSYP Q QPQ  H  ++S+PI++P+S +    PP   S
Sbjct: 145 AKRWSSQP--QSFAHSKPLYRTSSYPQQ-QPQLQH--YNSEPIILPESNFTSFPPPGNRS 204

Query: 188 PQASPNHVN-MPFVPGGRHVASLSPSNLTPPNSQIGGFNPGARFGGNMPQ-LSAGLSINS 247
           PQASP +++  P +PGG  +   +PS L+     + G + G  +GGN+ +  S G ++ +
Sbjct: 205 PQASPGNLHRAPSLPGGSQLTYSAPSPLSNSGFHLSGLSQGPHYGGNLTRYASCGPTLGN 264

Query: 248 GPPSQWNNQTGMFPGEHSSHLNNLLPRQLSNQNGFPQLLPQSQQQQQH--RLQQPPFGGS 307
                W    G   G+HS  L+NL+ +Q        QL P++    QH   LQQ      
Sbjct: 265 MVQPHWVTDPGHLHGDHSGLLHNLVQQQ------HQQLPPRNAIMSQHLLALQQRQSYAQ 324

Query: 308 LPGFQSHLFNSHISSGPPPLMNKLEAMLGGLPDMRDQRPKYVQKGRQNARFIHQGHENNS 367
           L   QS L++S+    P P       +  G+ ++R+ + K   + R+N     Q  +  S
Sbjct: 325 LAALQSQLYSSY----PSP----SRKVPFGVGEVREHKHKSSHRSRKNRGLSQQTSDAAS 384

Query: 368 FKNDFGWPFYRSKYMTTDELENIVRMQLAATHSNDPYVDDYYHQACLARKSAGAKLRHHF 427
            K++ G  F RSK+MT++E+E+I++MQ + +HSNDPYV+DYYHQA LA+KSAG+K   HF
Sbjct: 385 QKSETGLQF-RSKHMTSEEIESILKMQHSNSHSNDPYVNDYYHQAKLAKKSAGSKAISHF 444

Query: 428 CPNQLRDLPPRARANNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSDAGSTEQKIP 487
            P QL+D  PR+R ++E H  + V+ALG++   S+RRP  LLEVD     + GS + K  
Sbjct: 445 YPAQLKDHQPRSRNSSEQHPQVHVDALGKITLPSVRRPHALLEVDSSPGFNDGSGDHKGS 504

Query: 488 EKPLEQEPMLAARVTIEDGHCLLLDVDDIDRFLQFNQFQDSGA-LRRRRQVLLEGLAASF 547
            K LEQEP++AARVTIED   +L+D+ DIDR LQ  + QD GA L+R+RQ+LLEGLA + 
Sbjct: 505 GKHLEQEPLVAARVTIEDALGVLIDIVDIDRTLQNTRPQDGGAQLKRKRQILLEGLATAL 564

Query: 548 HIVDPLGKDGRAVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRIVCMAIFRH 607
            + DP  K G+  G+  KDD VFLR+ +LPKGRKLL KYLQLLVPG E  R+VCMAIFRH
Sbjct: 565 QLADPFSKTGQKSGMTAKDDIVFLRIATLPKGRKLLTKYLQLLVPGTENARVVCMAIFRH 624

Query: 608 LRFLFGSLPSDPTTADSVNDLARIVSLRTHGMDLGALSACLAAVVCSSEQPPLRPLGNPA 667
           LRFLFG LPSD   A+++++LA+ V++    MDL ALSACLAAVVCSSEQPPLRP+G+ A
Sbjct: 625 LRFLFGGLPSDTLAAETISNLAKAVTVCVQAMDLRALSACLAAVVCSSEQPPLRPIGSSA 684

Query: 668 GDGASLILKSVLERATELLTDPHAASNYNITHRALWQASFDEFFGLLTKYCVNKYDSIMQ 727
           GDGAS++L S+LERA E++  P     +  ++  LW+ASFDEFF LLTKYC +KYD+I  
Sbjct: 685 GDGASVVLISLLERAAEVVVVPRVM--HGNSNDGLWRASFDEFFNLLTKYCRSKYDTI-- 744

Query: 728 SLLRQSPQNAAAAVSDAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSMSV----- 774
                  QN  +A      AI +EMP E+LRASL HT++ QR  L++F ++  ++     
Sbjct: 745 -----RGQNQGSAADVLELAIKREMPAELLRASLRHTNDDQRNYLLNFGRKPSAISESAS 780

BLAST of MS004614 vs. ExPASy Swiss-Prot
Match: Q94C98 (Protein PAT1 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=PAT1H2 PE=2 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 1.1e-155
Identity = 367/812 (45.20%), Postives = 498/812 (61.33%), Query Frame = 0

Query: 6   ARFDASQYAFFGKDVMEEVELGGLEDEEDDALAGGIDEEEF-LFDK-ESEDFRPTSDIDD 65
           A FDASQY FFG+  +EEVELGGL+D  D  + G +D+EE+ LFDK E       SD+DD
Sbjct: 22  ALFDASQYEFFGQS-LEEVELGGLDD--DGTVRGHVDDEEYHLFDKREGAGLGSLSDMDD 81

Query: 66  LVTSFEKFNNVGGGPR--GVIG----GRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQ 125
           L T+F K N    GP+  GVIG    G   RESS+  +W ++  F++WL Q    +E  Q
Sbjct: 82  LATTFAKLNRNVTGPKHLGVIGDRGSGSFSRESSTATDWTQDNEFTSWLDQHTV-EEQVQ 141

Query: 126 EGKRWSSHPHSSSLAESTSLYRTSSYPDQPQPQQYHQQFSSDPILVPKSPY----PPTGM 185
           E   WSS P SS    S SLYRTSSYP Q   Q   Q +SS+PI+VP+S +     P   
Sbjct: 142 EAS-WSSQPQSS--PNSNSLYRTSSYPQQ---QTQLQHYSSEPIIVPESTFTSFPSPGKR 201

Query: 186 SPQASPNHVN-MPFVPGGRHVASLSPSNLTPPNS--------QIGGFNPG-ARFGGNMPQ 245
           S Q+SP+H++  P +PGG      S SN + PN+         + G + G + +G N+ +
Sbjct: 202 SQQSSPSHIHRAPSLPGG------SQSNFSAPNASPLSNSTFHLSGLSHGPSHYGNNLAR 261

Query: 246 -LSAGLSINS--GPPSQWNNQTGMFPGEHSSHLNNLLP----RQLSNQNGFPQLLPQSQQ 305
             S G ++ +    P  W    G+  G+HS+ L++L+     +QL  +NGF         
Sbjct: 262 YASCGPTLGNMVQQPPHWVTDPGLLHGDHSALLHSLMQQQHLQQLPPRNGF-------TS 321

Query: 306 QQQHRLQQPPFGGSLPGFQSHLFNSHISSGPPPLMNKLEAMLGGLPDMRDQRPKYVQKGR 365
           QQ   LQQ      L   QS L++S+ S     L         G+ ++R+ + K   + R
Sbjct: 322 QQLISLQQRQSLAHLAALQSQLYSSYPSPSHKALF--------GVGEVREHKHKSSHRSR 381

Query: 366 QNARFI-HQGHENNSFKNDFGWPFYRSKYMTTDELENIVRMQLAATHSNDPYVDDYYHQA 425
           +N   I  Q  +  S K++ G  F RSKYMT++E+E+I++MQ + +HS+DPYV+DYYHQA
Sbjct: 382 KNRGGISQQTSDLASQKSESGLQF-RSKYMTSEEIESILKMQHSNSHSSDPYVNDYYHQA 441

Query: 426 CLARKSAGAKLRHHFCPNQLRDLPPRARANNEPHAFLQVEALGRVPFSSIRRPRPLLEVD 485
            LA+KS+G++ +    P+ L+D   R+R +++    + V+ALG++   SI RPR LLEVD
Sbjct: 442 RLAKKSSGSRTKPQLYPSHLKDHQSRSRNSSDQQPQVHVDALGKITLPSICRPRALLEVD 501

Query: 486 PPSSSDAGSTEQKIPEKPLEQEPMLAARVTIEDGHCLLLDVDDIDRFLQFNQFQDSGA-L 545
            P SS           K LE EP++AARVTIED   +L+D+ DIDR LQFN+ QD GA L
Sbjct: 502 SPPSSG---------HKHLEDEPLVAARVTIEDAFGVLIDIVDIDRTLQFNRPQDGGAQL 561

Query: 546 RRRRQVLLEGLAASFHIVDPLGKDGRAVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVP 605
           RR+RQ+LLEGLA S  +VDP  K G+  GL  KDD VFLR+ +LPKGRKLL KYLQLLVP
Sbjct: 562 RRKRQILLEGLATSLQLVDPFSKTGQKTGLTTKDDIVFLRITTLPKGRKLLTKYLQLLVP 621

Query: 606 GGELMRIVCMAIFRHLRFLFGSLPSDPTTADSVNDLARIVSLRTHGMDLGALSACLAAVV 665
           G E+ R+VCMA+FRHLRFLFG LPSD   A+++ +LA+ V++    MDL ALSACLAAVV
Sbjct: 622 GTEIARVVCMAVFRHLRFLFGGLPSDSLAAETIANLAKAVTVCVQAMDLRALSACLAAVV 681

Query: 666 CSSEQPPLRPLGNPAGDGASLILKSVLERATELLTD--PHAASNYNITHRALWQASFDEF 725
           CSSEQPPLRP+G+ +GDGAS++L S+LERA E++    P   SN+   +  LW+ASFDEF
Sbjct: 682 CSSEQPPLRPIGSSSGDGASVVLVSLLERAAEVIVAVVPPRVSNHGNPNDGLWRASFDEF 741

Query: 726 FGLLTKYCVNKYDSIMQSLLRQSPQNAAAAVSDAAAAISQEMPVEVLRASLPHTDEHQRK 774
           F LLTKYC +KY++I      Q+  NAA  +     AI +EMP E+LRASL HT+E QR 
Sbjct: 742 FSLLTKYCRSKYETIH----GQNHDNAADVLE---LAIKREMPAELLRASLRHTNEDQRN 785

BLAST of MS004614 vs. ExPASy Swiss-Prot
Match: Q86TB9 (Protein PAT1 homolog 1 OS=Homo sapiens OX=9606 GN=PATL1 PE=1 SV=2)

HSP 1 Score: 58.9 bits (141), Expect = 2.9e-07
Identity = 107/451 (23.73%), Postives = 182/451 (40.35%), Query Frame = 0

Query: 179 PNHVNMPFVP--GGRHVASLSPSNL-TPPNSQIGGFN-----PGARFGGNMPQLSAGLSI 238
           P HV  P  P     +   +SP+ L + PNS + G       P         QL  G  +
Sbjct: 213 PVHVRPPMPPRYPAPYGERMSPNQLCSVPNSSLLGHPFPPSVPPVLSPLQRAQLLGGAQL 272

Query: 239 NSGPPSQWNNQTGMFPGEHSSHLNNLLPRQLSNQNGFPQLLPQSQQQQQHRLQQPPFGGS 298
             G  S   +Q    PG   S L  + P+ L  + G  Q+LP +                
Sbjct: 273 QPGRMSP--SQFARVPGFVGSPLAAMNPKLLQGRVG--QMLPPA---------------- 332

Query: 299 LPGFQSHLFNSHISSGPPPLMNKLEAMLGGLPDMRDQ----RPKYVQKGRQNARFIHQ-- 358
            PGF++  F++  S+ PPP  +        L ++R Q    RP       Q+ R +HQ  
Sbjct: 333 -PGFRA-FFSAPPSATPPPQQHP-PGPGPHLQNLRSQAPMFRPDTTHLHPQHRRLLHQRQ 392

Query: 359 -----GHENNSFKNDFGWPFYRSKY------------MTTDELENIVRMQLAATHSNDPY 418
                 H N +   D G   +RS +            M   E + + ++Q+    S DPY
Sbjct: 393 QQNRSQHRNLNGAGDRG--SHRSSHQDHLRKDPYANLMLQREKDWVSKIQMMQLQSTDPY 452

Query: 419 VDDYYHQACLAR--KSAGAKLRHHFCPNQLRDLPPRARANNEPHAFLQVE---ALGRVPF 478
           +DD+Y+Q    +  K + A+      P + R      +     HA+  V+   +LG++  
Sbjct: 453 LDDFYYQNYFEKLEKLSAAEEIQGDGPKKERTKLITPQVAKLEHAYKPVQFEGSLGKLTV 512

Query: 479 SSIRRPRPLLEVDPPSSSDAGSTEQKIPEKPLEQEPMLAARVTIEDGHCLLLDVDDIDRF 538
           SS+  PR +++    S S+   T++K       ++      V IE  + LLLDV+D +R 
Sbjct: 513 SSVNNPRKMIDAVVTSRSEDDETKEK-----QVRDKRRKTLVIIEKTYSLLLDVEDYERR 572

Query: 539 LQFNQFQDSGALRRRRQVLLEGLAASFHIVDPLGKDGRAVGLAPKDDFVFLRLVSLPKGR 594
              +  ++  AL   R+  +  +  +          G+  G     D  F++++ + KG+
Sbjct: 573 YLLSLEEERPALMDDRKHKICSMYDNLR--------GKLPGQERPSDDHFVQIMCIRKGK 623

BLAST of MS004614 vs. ExPASy Swiss-Prot
Match: Q5R8Q4 (Protein PAT1 homolog 1 OS=Pongo abelii OX=9601 GN=PATL1 PE=2 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 4.9e-07
Identity = 107/451 (23.73%), Postives = 183/451 (40.58%), Query Frame = 0

Query: 179 PNHVNMPFVP--GGRHVASLSPSNL-TPPNSQIGGFN-----PGARFGGNMPQLSAGLSI 238
           P HV  P  P     +   +SP+ L + PNS + G       P         QL  G  +
Sbjct: 213 PVHVRPPMPPRYPAPYGERMSPNQLCSVPNSSLLGHPFPPSVPPVLSPLQRAQLLGGAQL 272

Query: 239 NSGPPSQWNNQTGMFPGEHSSHLNNLLPRQLSNQNGFPQLLPQSQQQQQHRLQQPPFGGS 298
             G  S   +Q    PG   S L  + P+ L  + G  Q+LP +                
Sbjct: 273 QPGRMSP--SQFARVPGFVGSPLAAMNPKLLQGRVG--QMLPPA---------------- 332

Query: 299 LPGFQSHLFNSHISSGPPPLMNKLEAMLGGLPDMRDQ----RPKYVQKGRQNARFIHQ-- 358
            PGF++  F++  S+ PPP  +        L ++R Q    RP       Q+ R +HQ  
Sbjct: 333 -PGFRA-FFSAPPSATPPPQQHP-PGPGPHLQNLRSQAPMFRPDTTHLHPQHRRLLHQRQ 392

Query: 359 -----GHENNSFKNDFGWPFYRSKY------------MTTDELENIVRMQLAATHSNDPY 418
                 H N +   D G   +RS +            M   E + + ++Q+    S DPY
Sbjct: 393 QQNRNQHRNLNGAGDRG--SHRSSHQDHLRKDPYANLMLQREKDWVSKIQMMQLQSTDPY 452

Query: 419 VDDYYHQACLAR--KSAGAKLRHHFCPNQLRD--LPPRARANNEPHAFLQVE-ALGRVPF 478
           +DD+Y+Q    +  K + A+      P + R   + P+       +  +Q E +LG++  
Sbjct: 453 LDDFYYQNYFEKLEKLSAAEEIQGDGPKKERTKLITPQVAKLEHTYKPVQFEGSLGKLTV 512

Query: 479 SSIRRPRPLLEVDPPSSSDAGSTEQKIPEKPLEQEPMLAARVTIEDGHCLLLDVDDIDRF 538
           SS+  PR +++    S S+   T++K       ++      V IE  + LLLDV+D +R 
Sbjct: 513 SSVNNPRKMIDAVVTSRSEDDETKEK-----QVRDKRRKTLVIIEKTYSLLLDVEDYERR 572

Query: 539 LQFNQFQDSGALRRRRQVLLEGLAASFHIVDPLGKDGRAVGLAPKDDFVFLRLVSLPKGR 594
              +  ++  AL   R+  +  +  +          G+  G     D  F++++ + KG+
Sbjct: 573 YLLSLEEERPALMDERKHKICSMYDNLR--------GKLPGQERPSDDHFVQIMCIRKGK 623

BLAST of MS004614 vs. ExPASy TrEMBL
Match: A0A6J1C292 (protein PAT1 homolog 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007278 PE=4 SV=1)

HSP 1 Score: 1533.5 bits (3969), Expect = 0.0e+00
Identity = 767/773 (99.22%), Postives = 769/773 (99.48%), Query Frame = 0

Query: 1   NAVEDARFDASQYAFFGKDVMEEVELGGLEDEEDDALAGGIDEEEFLFDKESEDFRPTSD 60
           N+ EDARFDASQYAFFGKDVMEEVELGGLEDEEDDALAGGIDEEEFLFDKESEDFRPTSD
Sbjct: 26  NSAEDARFDASQYAFFGKDVMEEVELGGLEDEEDDALAGGIDEEEFLFDKESEDFRPTSD 85

Query: 61  IDDLVTSFEKFNNVGGGPRGVIGGRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQEGK 120
           IDDLVTSFEKFNNVGGGPRGVIGGRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQEGK
Sbjct: 86  IDDLVTSFEKFNNVGGGPRGVIGGRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQEGK 145

Query: 121 RWSSHPHSSSLAESTSLYRTSSYPDQPQPQQYHQQFSSDPILVPKSPYPPTGMSPQASPN 180
           RWSSHPHSSSLAESTSLYRTSSYPDQPQPQQYHQQFSSDPILVPKSPYPPTGMSPQASPN
Sbjct: 146 RWSSHPHSSSLAESTSLYRTSSYPDQPQPQQYHQQFSSDPILVPKSPYPPTGMSPQASPN 205

Query: 181 HVNMPFVPGGRHVASLSPSNLTPPNSQIGGFNPGARFGGNMPQLSAGLSINSGPPSQWNN 240
           HVNMPFVPGG HVASLSPSNLTPPN QIGGFNPGARFGGNMPQLSAGLSINSGPPSQWNN
Sbjct: 206 HVNMPFVPGGHHVASLSPSNLTPPNLQIGGFNPGARFGGNMPQLSAGLSINSGPPSQWNN 265

Query: 241 QTGMFPGEHSSHLNNLLPRQLSNQNGFPQLLPQSQQQQQHRLQQPPFGGSLPGFQSHLFN 300
           QTGMFPGEHSSHLNNLLPRQLSNQNGFPQLLPQSQQQQQHRLQQPPFGGSLPGFQSHLFN
Sbjct: 266 QTGMFPGEHSSHLNNLLPRQLSNQNGFPQLLPQSQQQQQHRLQQPPFGGSLPGFQSHLFN 325

Query: 301 SHISSGPPPLMNKLEAMLGGLPDMRDQRPKYVQKGRQNARFIHQGHENNSFKNDFGWPFY 360
           SHISSGPPPLMNKLEAMLGGLPDMRDQRPK VQKGRQNARFIHQGHENNSFKNDFGWPFY
Sbjct: 326 SHISSGPPPLMNKLEAMLGGLPDMRDQRPKSVQKGRQNARFIHQGHENNSFKNDFGWPFY 385

Query: 361 RSKYMTTDELENIVRMQLAATHSNDPYVDDYYHQACLARKSAGAKLRHHFCPNQLRDLPP 420
           RSKYMTTDELENIVRMQLAATHSNDPYVDDYYHQACLARKSAGAKLRHHFCPNQLRDLPP
Sbjct: 386 RSKYMTTDELENIVRMQLAATHSNDPYVDDYYHQACLARKSAGAKLRHHFCPNQLRDLPP 445

Query: 421 RARANNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSDAGSTEQKIPEKPLEQEPML 480
           RARANNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSDAG+TEQKIPEKPLEQEPML
Sbjct: 446 RARANNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSDAGTTEQKIPEKPLEQEPML 505

Query: 481 AARVTIEDGHCLLLDVDDIDRFLQFNQFQDSGALRRRRQVLLEGLAASFHIVDPLGKDGR 540
           AARVTIEDGHCLLLDVDDIDRFLQFNQFQDSGALRRRRQVLLEGLAASFHIVDPLGKDGR
Sbjct: 506 AARVTIEDGHCLLLDVDDIDRFLQFNQFQDSGALRRRRQVLLEGLAASFHIVDPLGKDGR 565

Query: 541 AVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRIVCMAIFRHLRFLFGSLPSD 600
           AVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRIVCMAIFRHLRFLFGSLPSD
Sbjct: 566 AVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRIVCMAIFRHLRFLFGSLPSD 625

Query: 601 PTTADSVNDLARIVSLRTHGMDLGALSACLAAVVCSSEQPPLRPLGNPAGDGASLILKSV 660
           PTTADSVNDLARIVSLRTHGMDLGALSACLAAVVCSSEQPPLRPLGNPAGDGASLILKSV
Sbjct: 626 PTTADSVNDLARIVSLRTHGMDLGALSACLAAVVCSSEQPPLRPLGNPAGDGASLILKSV 685

Query: 661 LERATELLTDPHAASNYNITHRALWQASFDEFFGLLTKYCVNKYDSIMQSLLRQSPQNAA 720
           LERATELLTDPHAASNYNITHRALWQASFDEFFGLLTKYCVNKYDSIMQSLLRQSPQNAA
Sbjct: 686 LERATELLTDPHAASNYNITHRALWQASFDEFFGLLTKYCVNKYDSIMQSLLRQSPQNAA 745

Query: 721 AAVSDAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSMSVGGRNNFESV 774
           AAVSDAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSMSVGGRNNFESV
Sbjct: 746 AAVSDAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSMSVGGRNNFESV 798

BLAST of MS004614 vs. ExPASy TrEMBL
Match: A0A6J1C087 (protein PAT1 homolog 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007278 PE=4 SV=1)

HSP 1 Score: 1528.8 bits (3957), Expect = 0.0e+00
Identity = 765/769 (99.48%), Postives = 766/769 (99.61%), Query Frame = 0

Query: 5   DARFDASQYAFFGKDVMEEVELGGLEDEEDDALAGGIDEEEFLFDKESEDFRPTSDIDDL 64
           DARFDASQYAFFGKDVMEEVELGGLEDEEDDALAGGIDEEEFLFDKESEDFRPTSDIDDL
Sbjct: 29  DARFDASQYAFFGKDVMEEVELGGLEDEEDDALAGGIDEEEFLFDKESEDFRPTSDIDDL 88

Query: 65  VTSFEKFNNVGGGPRGVIGGRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQEGKRWSS 124
           VTSFEKFNNVGGGPRGVIGGRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQEGKRWSS
Sbjct: 89  VTSFEKFNNVGGGPRGVIGGRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQEGKRWSS 148

Query: 125 HPHSSSLAESTSLYRTSSYPDQPQPQQYHQQFSSDPILVPKSPYPPTGMSPQASPNHVNM 184
           HPHSSSLAESTSLYRTSSYPDQPQPQQYHQQFSSDPILVPKSPYPPTGMSPQASPNHVNM
Sbjct: 149 HPHSSSLAESTSLYRTSSYPDQPQPQQYHQQFSSDPILVPKSPYPPTGMSPQASPNHVNM 208

Query: 185 PFVPGGRHVASLSPSNLTPPNSQIGGFNPGARFGGNMPQLSAGLSINSGPPSQWNNQTGM 244
           PFVPGG HVASLSPSNLTPPN QIGGFNPGARFGGNMPQLSAGLSINSGPPSQWNNQTGM
Sbjct: 209 PFVPGGHHVASLSPSNLTPPNLQIGGFNPGARFGGNMPQLSAGLSINSGPPSQWNNQTGM 268

Query: 245 FPGEHSSHLNNLLPRQLSNQNGFPQLLPQSQQQQQHRLQQPPFGGSLPGFQSHLFNSHIS 304
           FPGEHSSHLNNLLPRQLSNQNGFPQLLPQSQQQQQHRLQQPPFGGSLPGFQSHLFNSHIS
Sbjct: 269 FPGEHSSHLNNLLPRQLSNQNGFPQLLPQSQQQQQHRLQQPPFGGSLPGFQSHLFNSHIS 328

Query: 305 SGPPPLMNKLEAMLGGLPDMRDQRPKYVQKGRQNARFIHQGHENNSFKNDFGWPFYRSKY 364
           SGPPPLMNKLEAMLGGLPDMRDQRPK VQKGRQNARFIHQGHENNSFKNDFGWPFYRSKY
Sbjct: 329 SGPPPLMNKLEAMLGGLPDMRDQRPKSVQKGRQNARFIHQGHENNSFKNDFGWPFYRSKY 388

Query: 365 MTTDELENIVRMQLAATHSNDPYVDDYYHQACLARKSAGAKLRHHFCPNQLRDLPPRARA 424
           MTTDELENIVRMQLAATHSNDPYVDDYYHQACLARKSAGAKLRHHFCPNQLRDLPPRARA
Sbjct: 389 MTTDELENIVRMQLAATHSNDPYVDDYYHQACLARKSAGAKLRHHFCPNQLRDLPPRARA 448

Query: 425 NNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSDAGSTEQKIPEKPLEQEPMLAARV 484
           NNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSDAG+TEQKIPEKPLEQEPMLAARV
Sbjct: 449 NNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSDAGTTEQKIPEKPLEQEPMLAARV 508

Query: 485 TIEDGHCLLLDVDDIDRFLQFNQFQDSGALRRRRQVLLEGLAASFHIVDPLGKDGRAVGL 544
           TIEDGHCLLLDVDDIDRFLQFNQFQDSGALRRRRQVLLEGLAASFHIVDPLGKDGRAVGL
Sbjct: 509 TIEDGHCLLLDVDDIDRFLQFNQFQDSGALRRRRQVLLEGLAASFHIVDPLGKDGRAVGL 568

Query: 545 APKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRIVCMAIFRHLRFLFGSLPSDPTTA 604
           APKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRIVCMAIFRHLRFLFGSLPSDPTTA
Sbjct: 569 APKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRIVCMAIFRHLRFLFGSLPSDPTTA 628

Query: 605 DSVNDLARIVSLRTHGMDLGALSACLAAVVCSSEQPPLRPLGNPAGDGASLILKSVLERA 664
           DSVNDLARIVSLRTHGMDLGALSACLAAVVCSSEQPPLRPLGNPAGDGASLILKSVLERA
Sbjct: 629 DSVNDLARIVSLRTHGMDLGALSACLAAVVCSSEQPPLRPLGNPAGDGASLILKSVLERA 688

Query: 665 TELLTDPHAASNYNITHRALWQASFDEFFGLLTKYCVNKYDSIMQSLLRQSPQNAAAAVS 724
           TELLTDPHAASNYNITHRALWQASFDEFFGLLTKYCVNKYDSIMQSLLRQSPQNAAAAVS
Sbjct: 689 TELLTDPHAASNYNITHRALWQASFDEFFGLLTKYCVNKYDSIMQSLLRQSPQNAAAAVS 748

Query: 725 DAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSMSVGGRNNFESV 774
           DAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSMSVGGRNNFESV
Sbjct: 749 DAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSMSVGGRNNFESV 797

BLAST of MS004614 vs. ExPASy TrEMBL
Match: A0A6J1C0R0 (protein PAT1 homolog 1 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111007278 PE=4 SV=1)

HSP 1 Score: 1471.1 bits (3807), Expect = 0.0e+00
Identity = 743/773 (96.12%), Postives = 745/773 (96.38%), Query Frame = 0

Query: 1   NAVEDARFDASQYAFFGKDVMEEVELGGLEDEEDDALAGGIDEEEFLFDKESEDFRPTSD 60
           N+ EDARFDASQYAFFGKDVMEEVELGGLEDEEDDALAGGIDEEEFLFDKESEDFRPTSD
Sbjct: 26  NSAEDARFDASQYAFFGKDVMEEVELGGLEDEEDDALAGGIDEEEFLFDKESEDFRPTSD 85

Query: 61  IDDLVTSFEKFNNVGGGPRGVIGGRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQEGK 120
           IDDLVTSFEKFNNVGGGPRGVIGGRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQEGK
Sbjct: 86  IDDLVTSFEKFNNVGGGPRGVIGGRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQEGK 145

Query: 121 RWSSHPHSSSLAESTSLYRTSSYPDQPQPQQYHQQFSSDPILVPKSPYPPTGMSPQASPN 180
           RWSSHPHSSSLAESTSLYRTSSYPDQPQPQQYHQQFSSDPILVPKSPYPPTGMSPQASPN
Sbjct: 146 RWSSHPHSSSLAESTSLYRTSSYPDQPQPQQYHQQFSSDPILVPKSPYPPTGMSPQASPN 205

Query: 181 HVNMPFVPGGRHVASLSPSNLTPPNSQIGGFNPGARFGGNMPQLSAGLSINSGPPSQWNN 240
           H                          IGGFNPGARFGGNMPQLSAGLSINSGPPSQWNN
Sbjct: 206 H--------------------------IGGFNPGARFGGNMPQLSAGLSINSGPPSQWNN 265

Query: 241 QTGMFPGEHSSHLNNLLPRQLSNQNGFPQLLPQSQQQQQHRLQQPPFGGSLPGFQSHLFN 300
           QTGMFPGEHSSHLNNLLPRQLSNQNGFPQLLPQSQQQQQHRLQQPPFGGSLPGFQSHLFN
Sbjct: 266 QTGMFPGEHSSHLNNLLPRQLSNQNGFPQLLPQSQQQQQHRLQQPPFGGSLPGFQSHLFN 325

Query: 301 SHISSGPPPLMNKLEAMLGGLPDMRDQRPKYVQKGRQNARFIHQGHENNSFKNDFGWPFY 360
           SHISSGPPPLMNKLEAMLGGLPDMRDQRPK VQKGRQNARFIHQGHENNSFKNDFGWPFY
Sbjct: 326 SHISSGPPPLMNKLEAMLGGLPDMRDQRPKSVQKGRQNARFIHQGHENNSFKNDFGWPFY 385

Query: 361 RSKYMTTDELENIVRMQLAATHSNDPYVDDYYHQACLARKSAGAKLRHHFCPNQLRDLPP 420
           RSKYMTTDELENIVRMQLAATHSNDPYVDDYYHQACLARKSAGAKLRHHFCPNQLRDLPP
Sbjct: 386 RSKYMTTDELENIVRMQLAATHSNDPYVDDYYHQACLARKSAGAKLRHHFCPNQLRDLPP 445

Query: 421 RARANNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSDAGSTEQKIPEKPLEQEPML 480
           RARANNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSDAG+TEQKIPEKPLEQEPML
Sbjct: 446 RARANNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSDAGTTEQKIPEKPLEQEPML 505

Query: 481 AARVTIEDGHCLLLDVDDIDRFLQFNQFQDSGALRRRRQVLLEGLAASFHIVDPLGKDGR 540
           AARVTIEDGHCLLLDVDDIDRFLQFNQFQDSGALRRRRQVLLEGLAASFHIVDPLGKDGR
Sbjct: 506 AARVTIEDGHCLLLDVDDIDRFLQFNQFQDSGALRRRRQVLLEGLAASFHIVDPLGKDGR 565

Query: 541 AVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRIVCMAIFRHLRFLFGSLPSD 600
           AVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRIVCMAIFRHLRFLFGSLPSD
Sbjct: 566 AVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRIVCMAIFRHLRFLFGSLPSD 625

Query: 601 PTTADSVNDLARIVSLRTHGMDLGALSACLAAVVCSSEQPPLRPLGNPAGDGASLILKSV 660
           PTTADSVNDLARIVSLRTHGMDLGALSACLAAVVCSSEQPPLRPLGNPAGDGASLILKSV
Sbjct: 626 PTTADSVNDLARIVSLRTHGMDLGALSACLAAVVCSSEQPPLRPLGNPAGDGASLILKSV 685

Query: 661 LERATELLTDPHAASNYNITHRALWQASFDEFFGLLTKYCVNKYDSIMQSLLRQSPQNAA 720
           LERATELLTDPHAASNYNITHRALWQASFDEFFGLLTKYCVNKYDSIMQSLLRQSPQNAA
Sbjct: 686 LERATELLTDPHAASNYNITHRALWQASFDEFFGLLTKYCVNKYDSIMQSLLRQSPQNAA 745

Query: 721 AAVSDAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSMSVGGRNNFESV 774
           AAVSDAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSMSVGGRNNFESV
Sbjct: 746 AAVSDAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSMSVGGRNNFESV 772

BLAST of MS004614 vs. ExPASy TrEMBL
Match: A0A6J1IVH7 (uncharacterized protein LOC111480345 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111480345 PE=4 SV=1)

HSP 1 Score: 1286.6 bits (3328), Expect = 0.0e+00
Identity = 671/788 (85.15%), Postives = 706/788 (89.59%), Query Frame = 0

Query: 1   NAVEDARFDASQYAFFGKDVMEEVELGGLEDEEDDALAGGIDEEEFLFDKESEDFRPTSD 60
           N+ EDA FDASQYAFFGKD MEEVELGGLEDEEDD LA GIDEEEFLFDKESEDFRP SD
Sbjct: 34  NSTEDALFDASQYAFFGKD-MEEVELGGLEDEEDDTLAAGIDEEEFLFDKESEDFRPPSD 93

Query: 61  IDDLVTSFEKFNNVGGGPRGVIGGRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQEGK 120
           IDDLV+SFEK N++G GPRGVIGG +LRE+   NEWA E+GF NW+AQQ ++ E+AQEGK
Sbjct: 94  IDDLVSSFEKLNDIGSGPRGVIGGGLLREN---NEWACEDGFPNWIAQQGFNVETAQEGK 153

Query: 121 RWSSHPHSSSLAESTSLYRTSSYPD--QPQPQQYHQQFSSDPILVPKSPYPPTGMSPQAS 180
           RWSSHPH SSLAESTSLYRTSSYPD  QPQPQQYHQQFSS+PILVPKS YP +G+SP AS
Sbjct: 154 RWSSHPHPSSLAESTSLYRTSSYPDQPQPQPQQYHQQFSSEPILVPKSSYPSSGISPHAS 213

Query: 181 PN----HVNMPFVPGGRHVASLSPSNLTPPNSQIGGFNPGARFGGNMPQLSAGLSINSGP 240
           PN    H+NMPFVPGG HV SLSPSNLTPPNSQI GFN  +RFGGNMPQ S+G SIN GP
Sbjct: 214 PNQHPSHLNMPFVPGGHHVVSLSPSNLTPPNSQIAGFNSVSRFGGNMPQHSSGPSINGGP 273

Query: 241 PSQWNNQTGMFPGEHSSHLNNLLPRQLSNQNGFPQLLPQSQQQQQHRLQ---QPPFGGSL 300
            SQW NQTGMFPGEH SHLNNLLPRQLSNQNGFPQL PQ QQQQQHRLQ   QPPF GSL
Sbjct: 274 SSQWMNQTGMFPGEHVSHLNNLLPRQLSNQNGFPQLPPQ-QQQQQHRLQHPVQPPFVGSL 333

Query: 301 PGFQSHLFNSHISSGPPPLMNKLEAMLGGLPDMRDQRPKYVQKGRQNARFIHQGHENNSF 360
           PG Q+HL NSH SSGPP LMNKLEAML GLPDMRDQRP+  QKGRQ   + HQG+E N+F
Sbjct: 334 PGLQTHLLNSHSSSGPPHLMNKLEAML-GLPDMRDQRPR-SQKGRQTTHYAHQGYETNNF 393

Query: 361 KNDFGWPFYRSKYMTTDELENIVRMQLAATHSNDPYVDDYYHQACLARKSAGAKLRHHFC 420
           ++DFGWPF RSKYM   ELENIVR+QLAATHSNDPYVDDYYHQACLARKSAGAKLRHHFC
Sbjct: 394 RSDFGWPFRRSKYMKAYELENIVRIQLAATHSNDPYVDDYYHQACLARKSAGAKLRHHFC 453

Query: 421 PNQLRDLPPRARANNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSDAGSTEQKIPE 480
           PNQLRDLPP ARAN+EPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSS+ +GS +QK+ E
Sbjct: 454 PNQLRDLPPHARANSEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSTVSGSNDQKVSE 513

Query: 481 KPLEQEPMLAARVTIEDGHCLLLDVDDIDRFLQFNQFQDSGA-LRRRRQVLLEGLAASFH 540
           KPLEQEPMLAARVTIEDG CLLLDVDDIDRFLQFNQFQD GA LRRRRQVLLEGLAASFH
Sbjct: 514 KPLEQEPMLAARVTIEDGQCLLLDVDDIDRFLQFNQFQDGGAQLRRRRQVLLEGLAASFH 573

Query: 541 IVDPLGKDGRAVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRIVCMAIFRHL 600
           IVDPL KDG AVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMR VCMAIFRHL
Sbjct: 574 IVDPLSKDGHAVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRTVCMAIFRHL 633

Query: 601 RFLFGSLPSDPTTADSVNDLARIVSLRTHGMDLGALSACLAAVVCSSEQPPLRPLGNPAG 660
           RFLFGS   DP  ADSVNDLA+IVSLRTH MDLGALSACLAAVVCSSEQPPLRPLG PAG
Sbjct: 634 RFLFGS--PDPAAADSVNDLAKIVSLRTHSMDLGALSACLAAVVCSSEQPPLRPLGTPAG 693

Query: 661 DGASLILKSVLERATELLTDPHAASNYNITHRALWQASFDEFFGLLTKYCVNKYDSIMQS 720
           DGASLILKSVLERATELLTD HAASNYNITHRALWQASFDEFFG+LTKYCVNKYDSIMQS
Sbjct: 694 DGASLILKSVLERATELLTDRHAASNYNITHRALWQASFDEFFGILTKYCVNKYDSIMQS 753

Query: 721 LLRQSPQNAAAAVSDAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSMSVGG---- 774
           LLRQSPQNAA+AVSDAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSMSVGG    
Sbjct: 754 LLRQSPQNAASAVSDAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSMSVGGFNNS 812

BLAST of MS004614 vs. ExPASy TrEMBL
Match: A0A6J1FJ64 (uncharacterized protein LOC111445957 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445957 PE=4 SV=1)

HSP 1 Score: 1285.8 bits (3326), Expect = 0.0e+00
Identity = 671/789 (85.04%), Postives = 706/789 (89.48%), Query Frame = 0

Query: 1   NAVEDARFDASQYAFFGKDVMEEVELGGLEDEEDDALAGGIDEEEFLFDKESEDFRPTSD 60
           N+ EDA FDASQYAFFGKD MEEVELGGLEDEEDD LA GIDEEEFLFDKESEDFRP SD
Sbjct: 34  NSTEDALFDASQYAFFGKD-MEEVELGGLEDEEDDTLAAGIDEEEFLFDKESEDFRPPSD 93

Query: 61  IDDLVTSFEKFNNVGGGPRGVIGGRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQEGK 120
           IDDLV+SFEK N++G GPRGVIGG +LRE+S   EWA E+GF NW+AQQ ++ E+AQEGK
Sbjct: 94  IDDLVSSFEKLNDIGSGPRGVIGGGLLRENS---EWACEDGFPNWIAQQGFNVETAQEGK 153

Query: 121 RWSSHPHSSSLAESTSLYRTSSYPD--QPQPQQYHQQFSSDPILVPKSPYPPTGMSPQAS 180
           RWSSHPH SSLAESTSLYRTSSYPD  QPQPQQYHQQFSS+PILVPKS YP +G+SP AS
Sbjct: 154 RWSSHPHPSSLAESTSLYRTSSYPDQPQPQPQQYHQQFSSEPILVPKSSYPSSGISPHAS 213

Query: 181 PN----HVNMPFVPGGRHVASLSPSNLTPPNSQIGGFNPGARFGGNMPQLSAGLSINSGP 240
           PN    H+NMPFVPGG HV SLSPSNLTPPNSQI GFN  +RFGGNMPQ S+G SIN GP
Sbjct: 214 PNQHPSHLNMPFVPGGHHVLSLSPSNLTPPNSQIAGFNSVSRFGGNMPQHSSGPSINGGP 273

Query: 241 PSQWNNQTGMFPGEHSSHLNNLLPRQLSNQNGFPQLLP-QSQQQQQHRLQ---QPPFGGS 300
            SQW NQTGMFPGEH SHL+NLLPRQLSNQNGFPQL P Q QQQQQHRLQ   QPPF GS
Sbjct: 274 SSQWMNQTGMFPGEHVSHLSNLLPRQLSNQNGFPQLPPQQQQQQQQHRLQHPVQPPFVGS 333

Query: 301 LPGFQSHLFNSHISSGPPPLMNKLEAMLGGLPDMRDQRPKYVQKGRQNARFIHQGHENNS 360
           LPG Q+HLFNSH SSGPP LMNKLEAML GLPDMRDQRP+  QKGRQ A + HQ +E N+
Sbjct: 334 LPGLQTHLFNSHSSSGPPHLMNKLEAML-GLPDMRDQRPR-SQKGRQTAHYAHQSYETNN 393

Query: 361 FKNDFGWPFYRSKYMTTDELENIVRMQLAATHSNDPYVDDYYHQACLARKSAGAKLRHHF 420
           F++DFGWPF RSKYM   ELENIVR+QLAATHSNDPYVDDYYHQACLARKSAGAKLRHHF
Sbjct: 394 FRSDFGWPFRRSKYMKAFELENIVRIQLAATHSNDPYVDDYYHQACLARKSAGAKLRHHF 453

Query: 421 CPNQLRDLPPRARANNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSDAGSTEQKIP 480
           CPNQLRDLPP ARAN+EPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSS+  GS +QK+P
Sbjct: 454 CPNQLRDLPPHARANSEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSTVGGSNDQKVP 513

Query: 481 EKPLEQEPMLAARVTIEDGHCLLLDVDDIDRFLQFNQFQDSGA-LRRRRQVLLEGLAASF 540
           EKPLEQEPMLAARVTIEDG CLLLDVDDIDRFLQFNQFQD GA LRRRRQVLLEGLAASF
Sbjct: 514 EKPLEQEPMLAARVTIEDGQCLLLDVDDIDRFLQFNQFQDGGAQLRRRRQVLLEGLAASF 573

Query: 541 HIVDPLGKDGRAVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRIVCMAIFRH 600
           HIVDPL KDG AVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMR VCMAIFRH
Sbjct: 574 HIVDPLSKDGHAVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRTVCMAIFRH 633

Query: 601 LRFLFGSLPSDPTTADSVNDLARIVSLRTHGMDLGALSACLAAVVCSSEQPPLRPLGNPA 660
           LRFLFGS   DP  ADSVNDLA+IVS RTH MDLGALSACLAAVVCSSEQPPLRPLG PA
Sbjct: 634 LRFLFGS--PDPAAADSVNDLAKIVSSRTHSMDLGALSACLAAVVCSSEQPPLRPLGTPA 693

Query: 661 GDGASLILKSVLERATELLTDPHAASNYNITHRALWQASFDEFFGLLTKYCVNKYDSIMQ 720
           GDGASLILKSVLERATELLTD HAASNYNITHRALWQASFDEFFG+LTKYCVNKYDSIMQ
Sbjct: 694 GDGASLILKSVLERATELLTDRHAASNYNITHRALWQASFDEFFGILTKYCVNKYDSIMQ 753

Query: 721 SLLRQSPQNAAAAVSDAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSMSVGG--- 774
           SLLRQSPQNAA+AVSDAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSMSVGG   
Sbjct: 754 SLLRQSPQNAASAVSDAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSMSVGGFNN 813

BLAST of MS004614 vs. TAIR 10
Match: AT1G79090.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Topoisomerase II-associated protein PAT1 (InterPro:IPR019167); BEST Arabidopsis thaliana protein match is: Topoisomerase II-associated protein PAT1 (TAIR:AT3G22270.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 709.1 bits (1829), Expect = 3.8e-204
Identity = 415/779 (53.27%), Postives = 523/779 (67.14%), Query Frame = 0

Query: 1   NAVEDARFDASQYAFFGKDVMEEVELGGLEDEEDDALAGGIDEEEFLFDKES-EDFRPTS 60
           N+  +  FDASQYAFFG DV+EEVELGGLE+E++     GI  E+F FDKE   D R  S
Sbjct: 25  NSTGNTMFDASQYAFFGNDVVEEVELGGLEEEDEILSFTGI-AEDFSFDKEEVGDSRLLS 84

Query: 61  DIDDLVTSFEKFNNVGG--GPRGVIGGRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQ 120
           D+DDL ++F K N         G I  R   ++S   EW   E   NW  +Q  D ++ +
Sbjct: 85  DVDDLASTFSKLNREPDVYSNTGPITDRRSSQNSLAAEWTHGEELPNWYGRQILDSDAIK 144

Query: 121 EGKRWSSHPHSS-SLAESTSLYRTSSYPD-QPQPQQYH--QQFSSDPILVPKSPY----P 180
           + K WS+ P SS    E     RT  YP+ Q Q  Q H  QQFSS+PILVPKS +    P
Sbjct: 145 DDKVWSAQPFSSLDRVEQRIPDRTKLYPEPQRQLHQDHNQQQFSSEPILVPKSSFVSYPP 204

Query: 181 PTGMSPQASPNHVNMPFVPGGRHVASLSPSNLTPPNSQIGGFNPGA-RFGGNMPQLSAGL 240
           P  +SP     H N+P+  GG  + S + S       Q+   + G+ +  GN PQ    L
Sbjct: 205 PGSISPDQRLGHPNIPYQSGGPQMGSPNFSPFPNLQPQLPSMHHGSPQHTGNRPQFRPAL 264

Query: 241 SINSGPPSQWNNQTGMFPGEHSSHLNNLLPRQLSNQNGFPQLLPQSQQQQQHRL---QQP 300
            +N+ PP+QW N+  M PG+ S  +NN + +Q  +QNG   L+P   Q  Q+RL    QP
Sbjct: 265 PLNNLPPAQWMNRQNMHPGDSSGIMNNAMLQQPPHQNG---LMPPQMQGSQNRLPHPMQP 324

Query: 301 PFGGSLPGFQSHLFNSHISSGPPPLMNKLEAMLGGLPDMRDQRPKYVQKGRQNARFIHQG 360
           P  G +PG Q  LFNSH+S          + ML G  D+R+ RP      RQN RF  QG
Sbjct: 325 PL-GHMPGMQPQLFNSHLSRSSS--SGNYDGML-GFGDLREVRPGSGHGNRQNVRFPQQG 384

Query: 361 HENNSFKNDFGWPFYRSKYMTTDELENIVRMQLAATHSNDPYVDDYYHQACLARKSAGAK 420
            +    +    +PF RSKYM+  E+ENI+RMQL ATHSNDPYVDDYYHQACLA+KSAGAK
Sbjct: 385 FDAGVQRR---YPF-RSKYMSAGEIENILRMQLVATHSNDPYVDDYYHQACLAKKSAGAK 444

Query: 421 LRHHFCPNQLRDLPPRARANNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSDAGST 480
           L+HHFCPN LRDL  RAR+NNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPP+S+  G+ 
Sbjct: 445 LKHHFCPNHLRDLQQRARSNNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPNSAKFGNA 504

Query: 481 EQKIPEKPLEQEPMLAARVTIEDGHCLLLDVDDIDRFLQFNQFQDSG-ALRRRRQVLLEG 540
           E K  +KPL+QEPMLAARV IEDG CLLL+VDDIDRFL+FNQ QD G  L++RRQ LL+ 
Sbjct: 505 EHKPTDKPLDQEPMLAARVYIEDGLCLLLEVDDIDRFLEFNQLQDGGHQLKQRRQALLQS 564

Query: 541 LAASFHIVDPLGKDGRAVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRIVCM 600
           LA S  + DPL K+G++  L   DDF+FLR++SLPKGRKLL +YLQL+ PG +LMRIVCM
Sbjct: 565 LAVSLQLGDPLAKNGQSQSL---DDFLFLRVISLPKGRKLLIRYLQLIFPGSDLMRIVCM 624

Query: 601 AIFRHLRFLFGSLPSDPTTADSVNDLARIVSLRTHGMDLGALSACLAAVVCSSEQPPLRP 660
           AIFRHLR LFG L SDP    + N LA +++L    M+LG +S CLAAV CSSEQ PLRP
Sbjct: 625 AIFRHLRSLFGVLSSDPDIIKTTNKLATVINLCIQNMELGPVSTCLAAVSCSSEQAPLRP 684

Query: 661 LGNPAGDGASLILKSVLERATELLTDPHAASNYNITHRALWQASFDEFFGLLTKYCVNKY 720
           LG+P GDGAS +LKS+L+RA+EL+     A+N+N    ALW+ASF+EFF +L +YC++KY
Sbjct: 685 LGSPVGDGASTVLKSILDRASELI----RANNFNNAGIALWRASFNEFFNMLMRYCISKY 744

Query: 721 DSIMQSLLRQSPQNAAAAVS-DAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSM 763
           DSIMQSL  Q P + A  +S +AA AI +EMP+E+LR+S PH DE Q+++L++F +RSM
Sbjct: 745 DSIMQSL--QLPPHFATEISEEAAKAIVREMPIELLRSSFPHIDEQQKRILMEFLKRSM 782

BLAST of MS004614 vs. TAIR 10
Match: AT1G79090.2 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Topoisomerase II-associated protein PAT1 (InterPro:IPR019167); BEST Arabidopsis thaliana protein match is: Topoisomerase II-associated protein PAT1 (TAIR:AT3G22270.1); Has 1260 Blast hits to 1163 proteins in 186 species: Archae - 0; Bacteria - 32; Metazoa - 596; Fungi - 277; Plants - 212; Viruses - 0; Other Eukaryotes - 143 (source: NCBI BLink). )

HSP 1 Score: 709.1 bits (1829), Expect = 3.8e-204
Identity = 415/779 (53.27%), Postives = 523/779 (67.14%), Query Frame = 0

Query: 1   NAVEDARFDASQYAFFGKDVMEEVELGGLEDEEDDALAGGIDEEEFLFDKES-EDFRPTS 60
           N+  +  FDASQYAFFG DV+EEVELGGLE+E++     GI  E+F FDKE   D R  S
Sbjct: 25  NSTGNTMFDASQYAFFGNDVVEEVELGGLEEEDEILSFTGI-AEDFSFDKEEVGDSRLLS 84

Query: 61  DIDDLVTSFEKFNNVGG--GPRGVIGGRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQ 120
           D+DDL ++F K N         G I  R   ++S   EW   E   NW  +Q  D ++ +
Sbjct: 85  DVDDLASTFSKLNREPDVYSNTGPITDRRSSQNSLAAEWTHGEELPNWYGRQILDSDAIK 144

Query: 121 EGKRWSSHPHSS-SLAESTSLYRTSSYPD-QPQPQQYH--QQFSSDPILVPKSPY----P 180
           + K WS+ P SS    E     RT  YP+ Q Q  Q H  QQFSS+PILVPKS +    P
Sbjct: 145 DDKVWSAQPFSSLDRVEQRIPDRTKLYPEPQRQLHQDHNQQQFSSEPILVPKSSFVSYPP 204

Query: 181 PTGMSPQASPNHVNMPFVPGGRHVASLSPSNLTPPNSQIGGFNPGA-RFGGNMPQLSAGL 240
           P  +SP     H N+P+  GG  + S + S       Q+   + G+ +  GN PQ    L
Sbjct: 205 PGSISPDQRLGHPNIPYQSGGPQMGSPNFSPFPNLQPQLPSMHHGSPQHTGNRPQFRPAL 264

Query: 241 SINSGPPSQWNNQTGMFPGEHSSHLNNLLPRQLSNQNGFPQLLPQSQQQQQHRL---QQP 300
            +N+ PP+QW N+  M PG+ S  +NN + +Q  +QNG   L+P   Q  Q+RL    QP
Sbjct: 265 PLNNLPPAQWMNRQNMHPGDSSGIMNNAMLQQPPHQNG---LMPPQMQGSQNRLPHPMQP 324

Query: 301 PFGGSLPGFQSHLFNSHISSGPPPLMNKLEAMLGGLPDMRDQRPKYVQKGRQNARFIHQG 360
           P  G +PG Q  LFNSH+S          + ML G  D+R+ RP      RQN RF  QG
Sbjct: 325 PL-GHMPGMQPQLFNSHLSRSSS--SGNYDGML-GFGDLREVRPGSGHGNRQNVRFPQQG 384

Query: 361 HENNSFKNDFGWPFYRSKYMTTDELENIVRMQLAATHSNDPYVDDYYHQACLARKSAGAK 420
            +    +    +PF RSKYM+  E+ENI+RMQL ATHSNDPYVDDYYHQACLA+KSAGAK
Sbjct: 385 FDAGVQRR---YPF-RSKYMSAGEIENILRMQLVATHSNDPYVDDYYHQACLAKKSAGAK 444

Query: 421 LRHHFCPNQLRDLPPRARANNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSDAGST 480
           L+HHFCPN LRDL  RAR+NNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPP+S+  G+ 
Sbjct: 445 LKHHFCPNHLRDLQQRARSNNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPNSAKFGNA 504

Query: 481 EQKIPEKPLEQEPMLAARVTIEDGHCLLLDVDDIDRFLQFNQFQDSG-ALRRRRQVLLEG 540
           E K  +KPL+QEPMLAARV IEDG CLLL+VDDIDRFL+FNQ QD G  L++RRQ LL+ 
Sbjct: 505 EHKPTDKPLDQEPMLAARVYIEDGLCLLLEVDDIDRFLEFNQLQDGGHQLKQRRQALLQS 564

Query: 541 LAASFHIVDPLGKDGRAVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRIVCM 600
           LA S  + DPL K+G++  L   DDF+FLR++SLPKGRKLL +YLQL+ PG +LMRIVCM
Sbjct: 565 LAVSLQLGDPLAKNGQSQSL---DDFLFLRVISLPKGRKLLIRYLQLIFPGSDLMRIVCM 624

Query: 601 AIFRHLRFLFGSLPSDPTTADSVNDLARIVSLRTHGMDLGALSACLAAVVCSSEQPPLRP 660
           AIFRHLR LFG L SDP    + N LA +++L    M+LG +S CLAAV CSSEQ PLRP
Sbjct: 625 AIFRHLRSLFGVLSSDPDIIKTTNKLATVINLCIQNMELGPVSTCLAAVSCSSEQAPLRP 684

Query: 661 LGNPAGDGASLILKSVLERATELLTDPHAASNYNITHRALWQASFDEFFGLLTKYCVNKY 720
           LG+P GDGAS +LKS+L+RA+EL+     A+N+N    ALW+ASF+EFF +L +YC++KY
Sbjct: 685 LGSPVGDGASTVLKSILDRASELI----RANNFNNAGIALWRASFNEFFNMLMRYCISKY 744

Query: 721 DSIMQSLLRQSPQNAAAAVS-DAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSM 763
           DSIMQSL  Q P + A  +S +AA AI +EMP+E+LR+S PH DE Q+++L++F +RSM
Sbjct: 745 DSIMQSL--QLPPHFATEISEEAAKAIVREMPIELLRSSFPHIDEQQKRILMEFLKRSM 782

BLAST of MS004614 vs. TAIR 10
Match: AT3G22270.1 (Topoisomerase II-associated protein PAT1 )

HSP 1 Score: 582.4 bits (1500), Expect = 5.3e-166
Identity = 365/792 (46.09%), Postives = 499/792 (63.01%), Query Frame = 0

Query: 8   FDASQYAFFGKDVMEEVELGGLEDEEDDA-LAGGIDEEEF-LFDK-ESEDFRPTSDIDDL 67
           FDASQY FFG++ ++++ELGGL+D+   A + G  D++E+ LFDK E       SD+DDL
Sbjct: 25  FDASQYEFFGQN-LDDMELGGLDDDGVIAPVLGHADDDEYHLFDKGEGAGLGSLSDMDDL 84

Query: 68  VTSFEKFNNVGGGPR--GVIG----GRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQE 127
            T+F K N V  GP+  GVIG    G   RESSS  +W ++   ++WL      DE  QE
Sbjct: 85  ATTFAKLNRVVTGPKHPGVIGDRGSGSFSRESSSATDWTQDAELTSWL------DEQDQE 144

Query: 128 GKRWSSHPHSSSLAESTSLYRTSSYPDQPQPQQYHQQFSSDPILVPKSPY----PPTGMS 187
            KRWSS P   S A S  LYRTSSYP Q QPQ  H  ++S+PI++P+S +    PP   S
Sbjct: 145 AKRWSSQP--QSFAHSKPLYRTSSYPQQ-QPQLQH--YNSEPIILPESNFTSFPPPGNRS 204

Query: 188 PQASPNHVN-MPFVPGGRHVASLSPSNLTPPNSQIGGFNPGARFGGNMPQ-LSAGLSINS 247
           PQASP +++  P +PGG  +   +PS L+     + G + G  +GGN+ +  S G ++ +
Sbjct: 205 PQASPGNLHRAPSLPGGSQLTYSAPSPLSNSGFHLSGLSQGPHYGGNLTRYASCGPTLGN 264

Query: 248 GPPSQWNNQTGMFPGEHSSHLNNLLPRQLSNQNGFPQLLPQSQQQQQH--RLQQPPFGGS 307
                W    G   G+HS  L+NL+ +Q        QL P++    QH   LQQ      
Sbjct: 265 MVQPHWVTDPGHLHGDHSGLLHNLVQQQ------HQQLPPRNAIMSQHLLALQQRQSYAQ 324

Query: 308 LPGFQSHLFNSHISSGPPPLMNKLEAMLGGLPDMRDQRPKYVQKGRQNARFIHQGHENNS 367
           L   QS L++S+    P P       +  G+ ++R+ + K   + R+N     Q  +  S
Sbjct: 325 LAALQSQLYSSY----PSP----SRKVPFGVGEVREHKHKSSHRSRKNRGLSQQTSDAAS 384

Query: 368 FKNDFGWPFYRSKYMTTDELENIVRMQLAATHSNDPYVDDYYHQACLARKSAGAKLRHHF 427
            K++ G  F RSK+MT++E+E+I++MQ + +HSNDPYV+DYYHQA LA+KSAG+K   HF
Sbjct: 385 QKSETGLQF-RSKHMTSEEIESILKMQHSNSHSNDPYVNDYYHQAKLAKKSAGSKAISHF 444

Query: 428 CPNQLRDLPPRARANNEPHAFLQVEALGRVPFSSIRRPRPLLEVDPPSSSDAGSTEQKIP 487
            P QL+D  PR+R ++E H  + V+ALG++   S+RRP  LLEVD     + GS + K  
Sbjct: 445 YPAQLKDHQPRSRNSSEQHPQVHVDALGKITLPSVRRPHALLEVDSSPGFNDGSGDHKGS 504

Query: 488 EKPLEQEPMLAARVTIEDGHCLLLDVDDIDRFLQFNQFQDSGA-LRRRRQVLLEGLAASF 547
            K LEQEP++AARVTIED   +L+D+ DIDR LQ  + QD GA L+R+RQ+LLEGLA + 
Sbjct: 505 GKHLEQEPLVAARVTIEDALGVLIDIVDIDRTLQNTRPQDGGAQLKRKRQILLEGLATAL 564

Query: 548 HIVDPLGKDGRAVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVPGGELMRIVCMAIFRH 607
            + DP  K G+  G+  KDD VFLR+ +LPKGRKLL KYLQLLVPG E  R+VCMAIFRH
Sbjct: 565 QLADPFSKTGQKSGMTAKDDIVFLRIATLPKGRKLLTKYLQLLVPGTENARVVCMAIFRH 624

Query: 608 LRFLFGSLPSDPTTADSVNDLARIVSLRTHGMDLGALSACLAAVVCSSEQPPLRPLGNPA 667
           LRFLFG LPSD   A+++++LA+ V++    MDL ALSACLAAVVCSSEQPPLRP+G+ A
Sbjct: 625 LRFLFGGLPSDTLAAETISNLAKAVTVCVQAMDLRALSACLAAVVCSSEQPPLRPIGSSA 684

Query: 668 GDGASLILKSVLERATELLTDPHAASNYNITHRALWQASFDEFFGLLTKYCVNKYDSIMQ 727
           GDGAS++L S+LERA E++  P     +  ++  LW+ASFDEFF LLTKYC +KYD+I  
Sbjct: 685 GDGASVVLISLLERAAEVVVVPRVM--HGNSNDGLWRASFDEFFNLLTKYCRSKYDTI-- 744

Query: 728 SLLRQSPQNAAAAVSDAAAAISQEMPVEVLRASLPHTDEHQRKVLIDFAQRSMSV----- 774
                  QN  +A      AI +EMP E+LRASL HT++ QR  L++F ++  ++     
Sbjct: 745 -----RGQNQGSAADVLELAIKREMPAELLRASLRHTNDDQRNYLLNFGRKPSAISESAS 780

BLAST of MS004614 vs. TAIR 10
Match: AT4G14990.1 (Topoisomerase II-associated protein PAT1 )

HSP 1 Score: 552.0 bits (1421), Expect = 7.7e-157
Identity = 367/812 (45.20%), Postives = 498/812 (61.33%), Query Frame = 0

Query: 6   ARFDASQYAFFGKDVMEEVELGGLEDEEDDALAGGIDEEEF-LFDK-ESEDFRPTSDIDD 65
           A FDASQY FFG+  +EEVELGGL+D  D  + G +D+EE+ LFDK E       SD+DD
Sbjct: 22  ALFDASQYEFFGQS-LEEVELGGLDD--DGTVRGHVDDEEYHLFDKREGAGLGSLSDMDD 81

Query: 66  LVTSFEKFNNVGGGPR--GVIG----GRVLRESSSVNEWAREEGFSNWLAQQAYDDESAQ 125
           L T+F K N    GP+  GVIG    G   RESS+  +W ++  F++WL Q    +E  Q
Sbjct: 82  LATTFAKLNRNVTGPKHLGVIGDRGSGSFSRESSTATDWTQDNEFTSWLDQHTV-EEQVQ 141

Query: 126 EGKRWSSHPHSSSLAESTSLYRTSSYPDQPQPQQYHQQFSSDPILVPKSPY----PPTGM 185
           E   WSS P SS    S SLYRTSSYP Q   Q   Q +SS+PI+VP+S +     P   
Sbjct: 142 EAS-WSSQPQSS--PNSNSLYRTSSYPQQ---QTQLQHYSSEPIIVPESTFTSFPSPGKR 201

Query: 186 SPQASPNHVN-MPFVPGGRHVASLSPSNLTPPNS--------QIGGFNPG-ARFGGNMPQ 245
           S Q+SP+H++  P +PGG      S SN + PN+         + G + G + +G N+ +
Sbjct: 202 SQQSSPSHIHRAPSLPGG------SQSNFSAPNASPLSNSTFHLSGLSHGPSHYGNNLAR 261

Query: 246 -LSAGLSINS--GPPSQWNNQTGMFPGEHSSHLNNLLP----RQLSNQNGFPQLLPQSQQ 305
             S G ++ +    P  W    G+  G+HS+ L++L+     +QL  +NGF         
Sbjct: 262 YASCGPTLGNMVQQPPHWVTDPGLLHGDHSALLHSLMQQQHLQQLPPRNGF-------TS 321

Query: 306 QQQHRLQQPPFGGSLPGFQSHLFNSHISSGPPPLMNKLEAMLGGLPDMRDQRPKYVQKGR 365
           QQ   LQQ      L   QS L++S+ S     L         G+ ++R+ + K   + R
Sbjct: 322 QQLISLQQRQSLAHLAALQSQLYSSYPSPSHKALF--------GVGEVREHKHKSSHRSR 381

Query: 366 QNARFI-HQGHENNSFKNDFGWPFYRSKYMTTDELENIVRMQLAATHSNDPYVDDYYHQA 425
           +N   I  Q  +  S K++ G  F RSKYMT++E+E+I++MQ + +HS+DPYV+DYYHQA
Sbjct: 382 KNRGGISQQTSDLASQKSESGLQF-RSKYMTSEEIESILKMQHSNSHSSDPYVNDYYHQA 441

Query: 426 CLARKSAGAKLRHHFCPNQLRDLPPRARANNEPHAFLQVEALGRVPFSSIRRPRPLLEVD 485
            LA+KS+G++ +    P+ L+D   R+R +++    + V+ALG++   SI RPR LLEVD
Sbjct: 442 RLAKKSSGSRTKPQLYPSHLKDHQSRSRNSSDQQPQVHVDALGKITLPSICRPRALLEVD 501

Query: 486 PPSSSDAGSTEQKIPEKPLEQEPMLAARVTIEDGHCLLLDVDDIDRFLQFNQFQDSGA-L 545
            P SS           K LE EP++AARVTIED   +L+D+ DIDR LQFN+ QD GA L
Sbjct: 502 SPPSSG---------HKHLEDEPLVAARVTIEDAFGVLIDIVDIDRTLQFNRPQDGGAQL 561

Query: 546 RRRRQVLLEGLAASFHIVDPLGKDGRAVGLAPKDDFVFLRLVSLPKGRKLLGKYLQLLVP 605
           RR+RQ+LLEGLA S  +VDP  K G+  GL  KDD VFLR+ +LPKGRKLL KYLQLLVP
Sbjct: 562 RRKRQILLEGLATSLQLVDPFSKTGQKTGLTTKDDIVFLRITTLPKGRKLLTKYLQLLVP 621

Query: 606 GGELMRIVCMAIFRHLRFLFGSLPSDPTTADSVNDLARIVSLRTHGMDLGALSACLAAVV 665
           G E+ R+VCMA+FRHLRFLFG LPSD   A+++ +LA+ V++    MDL ALSACLAAVV
Sbjct: 622 GTEIARVVCMAVFRHLRFLFGGLPSDSLAAETIANLAKAVTVCVQAMDLRALSACLAAVV 681

Query: 666 CSSEQPPLRPLGNPAGDGASLILKSVLERATELLTD--PHAASNYNITHRALWQASFDEF 725
           CSSEQPPLRP+G+ +GDGAS++L S+LERA E++    P   SN+   +  LW+ASFDEF
Sbjct: 682 CSSEQPPLRPIGSSSGDGASVVLVSLLERAAEVIVAVVPPRVSNHGNPNDGLWRASFDEF 741

Query: 726 FGLLTKYCVNKYDSIMQSLLRQSPQNAAAAVSDAAAAISQEMPVEVLRASLPHTDEHQRK 774
           F LLTKYC +KY++I      Q+  NAA  +     AI +EMP E+LRASL HT+E QR 
Sbjct: 742 FSLLTKYCRSKYETIH----GQNHDNAADVLE---LAIKREMPAELLRASLRHTNEDQRN 785