MS004538 (gene) Bitter gourd (TR) v1

Overview


Name	MS004538
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	transcription factor IIIB 90 kDa subunit-like isoform X1
Location	scaffold995: 108863 .. 110725 (-)
RNA-Seq Expression	MS004538
Synteny	MS004538

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TATACTTTACAGATGAGGACTAAAGATGCATCATTATTCAGTATGTTTTTCAAATATAAGGATAAAAGTTCTCCATCCGATGACGAGTCAGAGGACATTGGCGATATTCTTGAAAGTGAGGTACCCTACTAAATTGCTTTAGCCTTTGGTTTCTTGAATTTCTGTTTTTGTTGGCATAGTTTTCTAACTGTAGTTTTTCTCAAATTACAGGTGAATCTTTATCTAAATAATGAGAAGGAGGCACGTTATAAAAAGATTATTTGGGAGAAAATTAACAAAGACTACCTTCAGGTTAAAGGCTTCTTAACCTTCACAATAACATTTTGATTGTTTCAAAGAAAGTCTTCACTCATGGTTACATTTAAAAACGGTGTTTTGCATTTCAGTCATTTAATTTCCAAAGAAATGCTATTCATGGTTTTCAGGAACAAGCAGCTAAGAAACAAGGTCGCGATGTTGCTGGAGCTGCACATGTGGTTAAAAAGTCAAAGAAGGTATTAAACTACTGTAGAAAAGAAGTTTCTTATCCAAGAAAAATATATTGGATAGGAAGTTCACTTGCATTTTAATGTATGATTTATGTTTTCTCACACTGTTGGAAAGCAGAGGCAAAGAAAAAAGGAAGCTGCAACCAATACCCCTGCTGAAGCTGATACAGAAGCTACACGTGAAATGCTAACCAAGAAGGTATATCAGCGGAAAAACTGATGAATACATTGTGATTTATTGCATTGAATCTGTTCCATTCTCAATTTTAATGTTTAAGTTTCTTAGAGTCTGATTGGTAACGTTATTGTTTCTTGTTTCTTTTTCTTCATTTTTAATAAAGAAAAATGAATAGTATGGCTCCAACATTCATGAATTCGAGTATGATACAACAATCTTTTCATTTATGTTTTGGAACAAATCGGTAATGGAAACAAAAAAAAAAAAACTTGTTTGGGTTGTTTCCAAAATTTCTATACAATTGTGAGAATGTTCCTAAAAAAGAGAAACAAAAAACAAAATTAGTTGCACAATGCTTAGATTTTTTGAAAGAAAATTGGAAATGAGGAACAAAAAACGGGAACGTTACCAAACAAGTCCTTAACTTCACATTCCTCAGGTGTATAACTTTCCAATTCACTTGGACCAACACTTTTCTGTTATTATTTTTCTCAAATAACTTTTTTAAGATTTAAGGTAATCCACCTGATTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTGGGGATTTATTTCCATAATTTATGGTTTTTGTGCATGCTAACTTAGATTGTTTGACTGTGTTTCATCGATTTAGTAATTGGCTTATATCCCACAACCTTTCTCTGCAGAGGCTTAGTTCGAAAGTCAACTTCGATGTGTTGGATGAACTCTTCTCTGACATGGTATGTTTATCAATTTGTTTCTTCTTTCATGTTCTGGTATGGTTAAGTTCAGCCTAGGATTCACACGTTCCAAGGTGACTGTAAATGGATTGTTAGAACAGGTAATTGGTAAATAACCATGCCCGAAGAAAAGGTGGAAAAAAAGGACACATAACTTTAACCCTTCTAAATCGTGATCAATTCGATTAGTACGCAGTCACACGGTTACTTATAACTGCCTAATAATTTATGTGCAGCCTGTATCTTGTTCAAACAAAAAGCAGGGAGCCTCGAACTGCGAAGAGAATAGTTCATCTGATCAAAGCCATTGTAGGGACCTCGAAGACAATGATCCCCATGCGGTGTTAGAAGAGGACGTTGACACAGAACATGAAGATCCCACAGAAACTTATGGTAACGAGTATTATGATATCGAAAACGAGGAAGAGTATGGTCATGTATATGGTGACTATGGTGGTGATGAGGAATAC

mRNA sequence

TATACTTTACAGATGAGGACTAAAGATGCATCATTATTCAGTATGTTTTTCAAATATAAGGATAAAAGTTCTCCATCCGATGACGAGTCAGAGGACATTGGCGATATTCTTGAAAGTGAGGTGAATCTTTATCTAAATAATGAGAAGGAGGCACGTTATAAAAAGATTATTTGGGAGAAAATTAACAAAGACTACCTTCAGGAACAAGCAGCTAAGAAACAAGGTCGCGATGTTGCTGGAGCTGCACATGTGGTTAAAAAGTCAAAGAAGAGGCAAAGAAAAAAGGAAGCTGCAACCAATACCCCTGCTGAAGCTGATACAGAAGCTACACGTGAAATGCTAACCAAGAAGAGGCTTAGTTCGAAAGTCAACTTCGATGTGTTGGATGAACTCTTCTCTGACATGCCTGTATCTTGTTCAAACAAAAAGCAGGGAGCCTCGAACTGCGAAGAGAATAGTTCATCTGATCAAAGCCATTGTAGGGACCTCGAAGACAATGATCCCCATGCGGTGTTAGAAGAGGACGTTGACACAGAACATGAAGATCCCACAGAAACTTATGGTAACGAGTATTATGATATCGAAAACGAGGAAGAGTATGGTCATGTATATGGTGACTATGGTGGTGATGAGGAATAC

Coding sequence (CDS)

TATACTTTACAGATGAGGACTAAAGATGCATCATTATTCAGTATGTTTTTCAAATATAAGGATAAAAGTTCTCCATCCGATGACGAGTCAGAGGACATTGGCGATATTCTTGAAAGTGAGGTGAATCTTTATCTAAATAATGAGAAGGAGGCACGTTATAAAAAGATTATTTGGGAGAAAATTAACAAAGACTACCTTCAGGAACAAGCAGCTAAGAAACAAGGTCGCGATGTTGCTGGAGCTGCACATGTGGTTAAAAAGTCAAAGAAGAGGCAAAGAAAAAAGGAAGCTGCAACCAATACCCCTGCTGAAGCTGATACAGAAGCTACACGTGAAATGCTAACCAAGAAGAGGCTTAGTTCGAAAGTCAACTTCGATGTGTTGGATGAACTCTTCTCTGACATGCCTGTATCTTGTTCAAACAAAAAGCAGGGAGCCTCGAACTGCGAAGAGAATAGTTCATCTGATCAAAGCCATTGTAGGGACCTCGAAGACAATGATCCCCATGCGGTGTTAGAAGAGGACGTTGACACAGAACATGAAGATCCCACAGAAACTTATGGTAACGAGTATTATGATATCGAAAACGAGGAAGAGTATGGTCATGTATATGGTGACTATGGTGGTGATGAGGAATAC

Protein sequence

YTLQMRTKDASLFSMFFKYKDKSSPSDDESEDIGDILESEVNLYLNNEKEARYKKIIWEKINKDYLQEQAAKKQGRDVAGAAHVVKKSKKRQRKKEAATNTPAEADTEATREMLTKKRLSSKVNFDVLDELFSDMPVSCSNKKQGASNCEENSSSDQSHCRDLEDNDPHAVLEEDVDTEHEDPTETYGNEYYDIENEEEYGHVYGDYGGDEEY

Homology

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BLAST of MS004538 vs. NCBI nr
Match: XP_022142780.1 (transcription factor IIIB 90 kDa subunit-like isoform X1 [Momordica charantia])

HSP 1 Score: 393.7 bits (1010), Expect = 1.0e-105
Identity = 207/209 (99.04%), Postives = 207/209 (99.04%), Query Frame = 0


Query: 5   MRTKDASLFSMFFKYKDKSSPSDDESEDIGDILESEVNLYLNNEKEARYKKIIWEKINKD 64
           MRTKDASLFSMFFK KDKSSPSDDESEDIGDILESEVNLYLNNEKEARYKKIIWEKINKD
Sbjct: 1   MRTKDASLFSMFFKXKDKSSPSDDESEDIGDILESEVNLYLNNEKEARYKKIIWEKINKD 60

Query: 65  YLQEQAAKKQGRDVAGAAHVVKKSKKRQRKKEAATNTPAEADTEATREMLTKKRLSSKVN 124
           YLQEQAAKKQGRDVAGAAHVVKKSKKRQRKKEAATNTPAEADTEATREMLTKKRLSSKVN
Sbjct: 61  YLQEQAAKKQGRDVAGAAHVVKKSKKRQRKKEAATNTPAEADTEATREMLTKKRLSSKVN 120

Query: 125 FDVLDELFSDMPVSCSNKKQGASNCEENSSSDQSHCRDLEDNDPHAVLEEDVDTEHEDPT 184
           FDVLDELFSDMPVSCSNKKQGASNCEENSS DQSHCRDLEDNDPHAVLEEDVDTEHEDPT
Sbjct: 121 FDVLDELFSDMPVSCSNKKQGASNCEENSSFDQSHCRDLEDNDPHAVLEEDVDTEHEDPT 180

Query: 185 ETYGNEYYDIENEEEYGHVYGDYGGDEEY 214
           ETYGNEYYDIENEEEYGHVYGDYGGDEEY
Sbjct: 181 ETYGNEYYDIENEEEYGHVYGDYGGDEEY 209

[Close]

BLAST of MS004538 vs. NCBI nr
Match: XP_022142859.1 (uncharacterized protein LOC111012793 isoform X2 [Momordica charantia])

HSP 1 Score: 329.7 bits (844), Expect = 1.8e-86
Identity = 172/174 (98.85%), Postives = 173/174 (99.43%), Query Frame = 0


Query: 40  EVNLYLNNEKEARYKKIIWEKINKDYLQEQAAKKQGRDVAGAAHVVKKSKKRQRKKEAAT 99
           +VNLYLNNEKEARYKKIIWEKINKDYLQEQAAKKQGRDVAGAAHVVKKSKKRQRKKEAAT
Sbjct: 12  KVNLYLNNEKEARYKKIIWEKINKDYLQEQAAKKQGRDVAGAAHVVKKSKKRQRKKEAAT 71

Query: 100 NTPAEADTEATREMLTKKRLSSKVNFDVLDELFSDMPVSCSNKKQGASNCEENSSSDQSH 159
           NTPAEADTEATREMLTKKRLSSKVNFDVLDELFSDMPVSCSNKKQGASNCEENSS DQSH
Sbjct: 72  NTPAEADTEATREMLTKKRLSSKVNFDVLDELFSDMPVSCSNKKQGASNCEENSSFDQSH 131

Query: 160 CRDLEDNDPHAVLEEDVDTEHEDPTETYGNEYYDIENEEEYGHVYGDYGGDEEY 214
           CRDLEDNDPHAVLEEDVDTEHEDPTETYGNEYYDIENEEEYGHVYGDYGGDEEY
Sbjct: 132 CRDLEDNDPHAVLEEDVDTEHEDPTETYGNEYYDIENEEEYGHVYGDYGGDEEY 185

[Close]

BLAST of MS004538 vs. NCBI nr
Match: XP_038896204.1 (transcription factor IIIB 60 kDa subunit-like [Benincasa hispida])

HSP 1 Score: 265.8 bits (678), Expect = 3.3e-67
Identity = 147/209 (70.33%), Postives = 171/209 (81.82%), Query Frame = 0


Query: 5   MRTKDASLFSMFFKYKDKSSPSDDESEDIGDILESEVNLYLNNEKEARYKKIIWEKINKD 64
           M+T DASLFSMFFK+KDK SPS++ESED+GD+L+ EV+ YLNN KEA YKKIIWEKINKD
Sbjct: 1   MKTVDASLFSMFFKHKDKPSPSNEESEDLGDVLDIEVDSYLNNRKEAHYKKIIWEKINKD 60

Query: 65  YLQEQAAKKQGRDVAGAAHVVKKSKKRQRKKEAATNTPAEADTEATREMLTKKRLSSKVN 124
           YLQ+Q AKK GRDV GAA ++KKSKKR R+KEA  N PA+A TEA+REMLTKKRLSSK N
Sbjct: 61  YLQDQTAKKLGRDVVGAAAMIKKSKKRPREKEAPINNPAQAGTEASREMLTKKRLSSKFN 120

Query: 125 FDVLDELFSDMPVSCSNKKQGASNCEENSSSDQSHCRDLEDNDPHAVLEEDVDTEHEDPT 184
           FDVLD+LFSD P   S +KQGAS+ +ENSSS+     DLEDN    + EE+VD E EDPT
Sbjct: 121 FDVLDKLFSDTPTPDSREKQGASSSKENSSSELE--VDLEDNHTDTMFEENVDIEGEDPT 180

Query: 185 ETYGNEYYDIENEEEYGHVYGDYGGDEEY 214
           ETYGNEYYD ENEEEYG+ + DYG +EEY
Sbjct: 181 ETYGNEYYDFENEEEYGYGFDDYGAEEEY 207

[Close]

BLAST of MS004538 vs. NCBI nr
Match: XP_008444162.1 (PREDICTED: uncharacterized protein LOC103487591 isoform X2 [Cucumis melo] >TYK31660.1 transcription factor IIIB 90 kDa subunit-like isoform X3 [Cucumis melo var. makuwa])

HSP 1 Score: 264.6 bits (675), Expect = 7.3e-67
Identity = 147/215 (68.37%), Postives = 172/215 (80.00%), Query Frame = 0


Query: 5   MRTKDASLFSMFFKYKDKSSPSDDESEDIGDILESEVNLYLNNEKEARYKKIIWEKINKD 64
           M+  DASLFSMF ++KDKSSPS+D+SED+GD+ +SEVN YLNN KEA YKKIIWE+INKD
Sbjct: 1   MKPADASLFSMFSRHKDKSSPSNDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKD 60

Query: 65  YLQEQAAKKQGRDVAGAAHVVKKSKKRQRKKEAATNTPAEADT-EATREMLTKKRLSSKV 124
           YLQ+QAAKKQG ++ GAA VVKKSKKR+RK EA  N PA+ADT E T EML KKRLSSK 
Sbjct: 61  YLQDQAAKKQGLNIVGAAAVVKKSKKRKRKIEAPINKPAQADTEETTHEMLIKKRLSSKF 120

Query: 125 NFDVLDELFSDMPVSCSNKKQGASNCEENSSSDQSHC-----RDLEDNDPHAVLEEDVDT 184
           +FDVLD+L  D P   S++KQGAS+C+ENSSS QSHC      D EDN    V EE++D 
Sbjct: 121 DFDVLDKLLYDTPAPDSSEKQGASSCKENSSSGQSHCINELEDDFEDNHTETVFEENLDI 180

Query: 185 EHEDPTETYGNEYYDIENEEEYGHVYGDYGGDEEY 214
           E EDPTETYGNEYYD +NEEEYG+ Y DYGG+EEY
Sbjct: 181 EGEDPTETYGNEYYDFQNEEEYGYGYDDYGGEEEY 215

[Close]

BLAST of MS004538 vs. NCBI nr
Match: XP_004142699.1 (uncharacterized protein LOC101217202 isoform X2 [Cucumis sativus])

HSP 1 Score: 264.2 bits (674), Expect = 9.5e-67
Identity = 150/236 (63.56%), Postives = 173/236 (73.31%), Query Frame = 0


Query: 5   MRTKDASLFSMFFKYKDKSSPSDDESEDIGDILESEVNLYLNNEKEARYKKIIWEKINKD 64
           M+T DASLF MFFK+KDKSSPSDD+SED+GD+ +SEVN YLNN KEA YKKIIWE+INKD
Sbjct: 1   MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKD 60

Query: 65  YLQEQAAKKQGRDVAGAAHVVKKSKKRQRKKEAATNTPAEADTEATREMLTKKRLSSKVN 124
           YLQ+QAAKKQG +V GA+ VVKKSKKRQRK EA  N PA+ADT  TREM  KKRLSSK N
Sbjct: 61  YLQDQAAKKQGLNVVGASAVVKKSKKRQRKTEAPINMPAQADTGTTREMQIKKRLSSKFN 120

Query: 125 FDVLDELFSDMPVSCSNKKQGASNCEENSSSDQSHCR----------------------- 184
           FDVLD+LFSD P   S++KQGAS+C+ENSSS Q HCR                       
Sbjct: 121 FDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNE 180

Query: 185 ---DLEDNDPHAVLEEDVDTE-HEDPTETYGNEYYDIENEEEYGHVYGDYGGDEEY 214
              + EDN    + EE+ D E  EDPTETYGNEYYD ++EEEYG+ Y DYGG+EEY
Sbjct: 181 LEDNFEDNHTETMFEENFDIEGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY 236

[Close]

BLAST of MS004538 vs. ExPASy Swiss-Prot
Match: Q8CFK2 (Transcription factor IIIB 90 kDa subunit OS=Mus musculus OX=10090 GN=Brf1 PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 9.7e-06
Identity = 44/125 (35.20%), Postives = 65/125 (52.00%), Query Frame = 0


Query: 23  SSPSDDESEDIGD-------ILESEVNLYLNNEKEARYKKIIWEKINKDYLQEQAAKKQG 82
           SSPS D  +  GD       I + E++ Y+ NE EAR K  +W + N +YL+EQ  K+  
Sbjct: 431 SSPSGDPKDSSGDGELDLSGIDDLEIDRYILNESEARVKAELWMRENAEYLREQKEKE-- 490

Query: 83  RDVAGAAHVVKKS-----KKRQRKKEAATNTPAEADT--EATREMLTKKRLSSKVNFDVL 134
                 A + K+      K+ + KK      P  A T  EA  +ML +K++SSK+N+ VL
Sbjct: 491 ------ARIAKEKELGIYKEHKPKKSCKRREPILASTAGEAIEKMLEQKKISSKINYSVL 547

[Close]

BLAST of MS004538 vs. ExPASy Swiss-Prot
Match: Q92994 (Transcription factor IIIB 90 kDa subunit OS=Homo sapiens OX=9606 GN=BRF1 PE=1 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 1.3e-05
Identity = 41/120 (34.17%), Postives = 62/120 (51.67%), Query Frame = 0


Query: 21  DKSSPSDDESEDIGDILESEVNLYLNNEKEARYKKIIWEKINKDYLQEQAAKKQGRDVAG 80
           D    S D   D+  I + E++ Y+ NE EAR K  +W + N +YL+EQ  K+       
Sbjct: 437 DPKDASGDGELDLSGIDDLEIDRYILNESEARVKAELWMRENAEYLREQREKE------- 496

Query: 81  AAHVVKKS-----KKRQRKKEAATNTPAEADT--EATREMLTKKRLSSKVNFDVLDELFS 134
            A + K+      K+ + KK      P +A T  EA  +ML +K++SSK+N+ VL  L S
Sbjct: 497 -ARIAKEKELGIYKEHKPKKSCKRREPIQASTAREAIEKMLEQKKISSKINYSVLRGLSS 548

[Close]

BLAST of MS004538 vs. ExPASy Swiss-Prot
Match: Q9P6R0 (Transcription factor IIIB 60 kDa subunit OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=brf1 PE=1 SV=2)

HSP 1 Score: 48.5 bits (114), Expect = 1.1e-04
Identity = 32/109 (29.36%), Postives = 60/109 (55.05%), Query Frame = 0


Query: 28  DESEDIGDILESEVNLYLNNEKEARYKKIIWEKINKDYLQEQAAKKQGRDVAGAAHVVKK 87
           +E   + D+ + E+   L ++ E   K  +W ++NKDYL E+ AK           +V++
Sbjct: 381 EEEVTLEDVDDDEIEDILLDKDEILTKTQVWMELNKDYLAEEEAKNLKLQEDLKKGIVRQ 440

Query: 88  SKKRQRKKEAATNTPAEADT--EATREMLTKKRLSSKVNFDVLDELFSD 135
            +KR+R +   + +   ADT  E+ +EM+ ++  S K+N++ LD LF +
Sbjct: 441 PRKRRRYRPRDSTSDGIADTAAESAKEMMQQRAFSKKINYEALDMLFDE 489

[Close]

BLAST of MS004538 vs. ExPASy TrEMBL
Match: A0A6J1CLW7 (transcription factor IIIB 90 kDa subunit-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111012793 PE=4 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 5.0e-106
Identity = 207/209 (99.04%), Postives = 207/209 (99.04%), Query Frame = 0


Query: 5   MRTKDASLFSMFFKYKDKSSPSDDESEDIGDILESEVNLYLNNEKEARYKKIIWEKINKD 64
           MRTKDASLFSMFFK KDKSSPSDDESEDIGDILESEVNLYLNNEKEARYKKIIWEKINKD
Sbjct: 1   MRTKDASLFSMFFKXKDKSSPSDDESEDIGDILESEVNLYLNNEKEARYKKIIWEKINKD 60

Query: 65  YLQEQAAKKQGRDVAGAAHVVKKSKKRQRKKEAATNTPAEADTEATREMLTKKRLSSKVN 124
           YLQEQAAKKQGRDVAGAAHVVKKSKKRQRKKEAATNTPAEADTEATREMLTKKRLSSKVN
Sbjct: 61  YLQEQAAKKQGRDVAGAAHVVKKSKKRQRKKEAATNTPAEADTEATREMLTKKRLSSKVN 120

Query: 125 FDVLDELFSDMPVSCSNKKQGASNCEENSSSDQSHCRDLEDNDPHAVLEEDVDTEHEDPT 184
           FDVLDELFSDMPVSCSNKKQGASNCEENSS DQSHCRDLEDNDPHAVLEEDVDTEHEDPT
Sbjct: 121 FDVLDELFSDMPVSCSNKKQGASNCEENSSFDQSHCRDLEDNDPHAVLEEDVDTEHEDPT 180

Query: 185 ETYGNEYYDIENEEEYGHVYGDYGGDEEY 214
           ETYGNEYYDIENEEEYGHVYGDYGGDEEY
Sbjct: 181 ETYGNEYYDIENEEEYGHVYGDYGGDEEY 209

[Close]

BLAST of MS004538 vs. ExPASy TrEMBL
Match: A0A6J1CMP1 (uncharacterized protein LOC111012793 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111012793 PE=4 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 8.9e-87
Identity = 172/174 (98.85%), Postives = 173/174 (99.43%), Query Frame = 0


Query: 40  EVNLYLNNEKEARYKKIIWEKINKDYLQEQAAKKQGRDVAGAAHVVKKSKKRQRKKEAAT 99
           +VNLYLNNEKEARYKKIIWEKINKDYLQEQAAKKQGRDVAGAAHVVKKSKKRQRKKEAAT
Sbjct: 12  KVNLYLNNEKEARYKKIIWEKINKDYLQEQAAKKQGRDVAGAAHVVKKSKKRQRKKEAAT 71

Query: 100 NTPAEADTEATREMLTKKRLSSKVNFDVLDELFSDMPVSCSNKKQGASNCEENSSSDQSH 159
           NTPAEADTEATREMLTKKRLSSKVNFDVLDELFSDMPVSCSNKKQGASNCEENSS DQSH
Sbjct: 72  NTPAEADTEATREMLTKKRLSSKVNFDVLDELFSDMPVSCSNKKQGASNCEENSSFDQSH 131

Query: 160 CRDLEDNDPHAVLEEDVDTEHEDPTETYGNEYYDIENEEEYGHVYGDYGGDEEY 214
           CRDLEDNDPHAVLEEDVDTEHEDPTETYGNEYYDIENEEEYGHVYGDYGGDEEY
Sbjct: 132 CRDLEDNDPHAVLEEDVDTEHEDPTETYGNEYYDIENEEEYGHVYGDYGGDEEY 185

[Close]

BLAST of MS004538 vs. ExPASy TrEMBL
Match: A0A5D3E7S6 (Transcription factor IIIB 90 kDa subunit-like isoform X3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold398G00100 PE=4 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 3.5e-67
Identity = 147/215 (68.37%), Postives = 172/215 (80.00%), Query Frame = 0


Query: 5   MRTKDASLFSMFFKYKDKSSPSDDESEDIGDILESEVNLYLNNEKEARYKKIIWEKINKD 64
           M+  DASLFSMF ++KDKSSPS+D+SED+GD+ +SEVN YLNN KEA YKKIIWE+INKD
Sbjct: 1   MKPADASLFSMFSRHKDKSSPSNDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKD 60

Query: 65  YLQEQAAKKQGRDVAGAAHVVKKSKKRQRKKEAATNTPAEADT-EATREMLTKKRLSSKV 124
           YLQ+QAAKKQG ++ GAA VVKKSKKR+RK EA  N PA+ADT E T EML KKRLSSK 
Sbjct: 61  YLQDQAAKKQGLNIVGAAAVVKKSKKRKRKIEAPINKPAQADTEETTHEMLIKKRLSSKF 120

Query: 125 NFDVLDELFSDMPVSCSNKKQGASNCEENSSSDQSHC-----RDLEDNDPHAVLEEDVDT 184
           +FDVLD+L  D P   S++KQGAS+C+ENSSS QSHC      D EDN    V EE++D 
Sbjct: 121 DFDVLDKLLYDTPAPDSSEKQGASSCKENSSSGQSHCINELEDDFEDNHTETVFEENLDI 180

Query: 185 EHEDPTETYGNEYYDIENEEEYGHVYGDYGGDEEY 214
           E EDPTETYGNEYYD +NEEEYG+ Y DYGG+EEY
Sbjct: 181 EGEDPTETYGNEYYDFQNEEEYGYGYDDYGGEEEY 215

[Close]

BLAST of MS004538 vs. ExPASy TrEMBL
Match: A0A1S3B993 (uncharacterized protein LOC103487591 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103487591 PE=4 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 3.5e-67
Identity = 147/215 (68.37%), Postives = 172/215 (80.00%), Query Frame = 0


Query: 5   MRTKDASLFSMFFKYKDKSSPSDDESEDIGDILESEVNLYLNNEKEARYKKIIWEKINKD 64
           M+  DASLFSMF ++KDKSSPS+D+SED+GD+ +SEVN YLNN KEA YKKIIWE+INKD
Sbjct: 1   MKPADASLFSMFSRHKDKSSPSNDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKD 60

Query: 65  YLQEQAAKKQGRDVAGAAHVVKKSKKRQRKKEAATNTPAEADT-EATREMLTKKRLSSKV 124
           YLQ+QAAKKQG ++ GAA VVKKSKKR+RK EA  N PA+ADT E T EML KKRLSSK 
Sbjct: 61  YLQDQAAKKQGLNIVGAAAVVKKSKKRKRKIEAPINKPAQADTEETTHEMLIKKRLSSKF 120

Query: 125 NFDVLDELFSDMPVSCSNKKQGASNCEENSSSDQSHC-----RDLEDNDPHAVLEEDVDT 184
           +FDVLD+L  D P   S++KQGAS+C+ENSSS QSHC      D EDN    V EE++D 
Sbjct: 121 DFDVLDKLLYDTPAPDSSEKQGASSCKENSSSGQSHCINELEDDFEDNHTETVFEENLDI 180

Query: 185 EHEDPTETYGNEYYDIENEEEYGHVYGDYGGDEEY 214
           E EDPTETYGNEYYD +NEEEYG+ Y DYGG+EEY
Sbjct: 181 EGEDPTETYGNEYYDFQNEEEYGYGYDDYGGEEEY 215

[Close]

BLAST of MS004538 vs. ExPASy TrEMBL
Match: A0A0A0L0J7 (BRF1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G363410 PE=4 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 4.6e-67
Identity = 150/236 (63.56%), Postives = 173/236 (73.31%), Query Frame = 0


Query: 5   MRTKDASLFSMFFKYKDKSSPSDDESEDIGDILESEVNLYLNNEKEARYKKIIWEKINKD 64
           M+T DASLF MFFK+KDKSSPSDD+SED+GD+ +SEVN YLNN KEA YKKIIWE+INKD
Sbjct: 61  MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKD 120

Query: 65  YLQEQAAKKQGRDVAGAAHVVKKSKKRQRKKEAATNTPAEADTEATREMLTKKRLSSKVN 124
           YLQ+QAAKKQG +V GA+ VVKKSKKRQRK EA  N PA+ADT  TREM  KKRLSSK N
Sbjct: 121 YLQDQAAKKQGLNVVGASAVVKKSKKRQRKTEAPINMPAQADTGTTREMQIKKRLSSKFN 180

Query: 125 FDVLDELFSDMPVSCSNKKQGASNCEENSSSDQSHCR----------------------- 184
           FDVLD+LFSD P   S++KQGAS+C+ENSSS Q HCR                       
Sbjct: 181 FDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNE 240

Query: 185 ---DLEDNDPHAVLEEDVDTE-HEDPTETYGNEYYDIENEEEYGHVYGDYGGDEEY 214
              + EDN    + EE+ D E  EDPTETYGNEYYD ++EEEYG+ Y DYGG+EEY
Sbjct: 241 LEDNFEDNHTETMFEENFDIEGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY 296

[Close]

BLAST of MS004538 vs. TAIR 10
Match: AT3G09360.1 (Cyclin/Brf1-like TBP-binding protein )

HSP 1 Score: 80.9 bits (198), Expect = 1.4e-15
Identity = 74/221 (33.48%), Postives = 107/221 (48.42%), Query Frame = 0


Query: 21  DKSSPSDDESEDIGDILESEVNLYLNNEKEARYKKIIWEKINKDYLQEQAAKKQGRDV-- 80
           ++ + + DES++  DI + EVN Y+NNE+E  YK I W ++NKDYL+EQAAK+       
Sbjct: 387 EEHADTSDESDNFSDISDDEVNGYINNEEETHYKTITWTEMNKDYLEEQAAKEAALKAAS 446

Query: 81  -------------------AGAAHVVKKSKKRQRKK--EAATNTPAEADTEATREMLTKK 140
                              A  A   K  K++Q+KK  EA    P     EA R  L KK
Sbjct: 447 EALKASNSNCPEDARKAFEAAKADAAKSRKEKQQKKAEEAKNAAPPATAVEAVRRTLDKK 506

Query: 141 RLSSKVNFDVLDELFSDMPVSCSNKKQGASNCEENSSSDQSHCRDLEDNDPHAVLEEDVD 200
           RLSS +N+DVL+ LF     S   K    S  E +    +   ++++ N+      ED D
Sbjct: 507 RLSSVINYDVLESLFD---TSAPEKSPKRSKTETDIEKKKEENKEMKSNEHENGENEDED 566

Query: 201 TEHEDP--TETY--------GNEYY-DIENEEEYGHVYGDY 208
            E E+    E+Y        G ++Y + E EEE G+ +G Y
Sbjct: 567 EEDEEEGNVESYDMKTDFQNGEKFYEEDEEEEEDGNDFGLY 604

[Close]

BLAST of MS004538 vs. TAIR 10
Match: AT4G19550.1 (zinc ion binding;transcription regulators )

HSP 1 Score: 48.5 bits (114), Expect = 7.6e-06
Identity = 56/187 (29.95%), Postives = 88/187 (47.06%), Query Frame = 0


Query: 28  DESEDIGDILESEVNLYLNNEKEARYKKIIWEKINKDYLQEQAAKKQGRDVAGAAHVVKK 87
           D +ED+ DI ++EV   L+N KE   KK  WE +N +Y + +  K   R     A+ +  
Sbjct: 30  DLAEDLSDISDAEVAENLSNNKEFILKKRAWELMNPEYQKGKYRKVTTRKKKDPANKIAP 89

Query: 88  SKKRQRKKEAATNTPAEADTEATREMLTKKRLSSKVNFDVLDELFS----------DMPV 147
           S     KK +AT T + A+TE       KKR S ++N++VLD+LF           D PV
Sbjct: 90  S-----KKTSATKTESNAETE------NKKRPSLEINYNVLDKLFDPENSPKRAKLDKPV 149

Query: 148 SCSNKKQGASNCEENSSSDQSHCRDLEDNDPHAVLEEDVDTE--HEDPTETYGN---EYY 200
              ++ + +    E S     +  + E+        ED   E  +    E YGN   E+ 
Sbjct: 150 VVGDQIEYSKQNSEESLLKSQYSEEEEEEAEEPDWNEDYSNEDAYRGNEECYGNENLEFE 205

[Close]

BLAST of MS004538 vs. TAIR 10
Match: AT2G01280.1 (Cyclin/Brf1-like TBP-binding protein )

HSP 1 Score: 42.4 bits (98), Expect = 5.5e-04
Identity = 65/201 (32.34%), Postives = 85/201 (42.29%), Query Frame = 0


Query: 21  DKSSPSDDESEDIGDILESEVNLYLNNEKEARYKKIIWEKINKDYLQEQAAKKQGRDVAG 80
           ++ +   DES+   D  +SEV   L  E E R K   W   NKDYL+EQA K+     A 
Sbjct: 368 ERHAEYSDESDICSDDDDSEVEHVLLGEDETRLKTTAWNLQNKDYLEEQAEKEAALKAAN 427

Query: 81  ----AAHVVKKSK---------KRQRKKEAATNTPAEADTEATREMLTKKRLSSKVN-FD 140
               A ++V+ SK         KR+++ E A N P  A   AT    T+   + +VN   
Sbjct: 428 CPEDARNLVEASKAAVANSRKEKRRKRAEEAKNAPPSA--TATEASYTE---TQRVNQHH 487

Query: 141 VLDELFSDMPVSCSNKKQGASNCEENSSSDQSHCRDLEDNDPHAVLEEDVDTEHEDPTET 200
           VLDEL    P   + KK       E    +     D ED       EED    H D    
Sbjct: 488 VLDELLDTSP---TQKKPRTETVTEKKKEEHEIVEDEED-------EEDYAAPHADE--- 547

Query: 201 YGNEYYDIENEEEYGHVYGDY 208
             N Y D   EEE G+ +G Y
Sbjct: 548 --NFYEDEVEEEENGYDFGLY 548

The following BLAST results are available for this feature:

BLAST of MS004538 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142780.1	1.0e-105	99.04	transcription factor IIIB 90 kDa subunit-like isoform X1 [Momordica charantia]	[more]
XP_022142859.1	1.8e-86	98.85	uncharacterized protein LOC111012793 isoform X2 [Momordica charantia]	[more]
XP_038896204.1	3.3e-67	70.33	transcription factor IIIB 60 kDa subunit-like [Benincasa hispida]	[more]
XP_008444162.1	7.3e-67	68.37	PREDICTED: uncharacterized protein LOC103487591 isoform X2 [Cucumis melo] >TYK31...	[more]
XP_004142699.1	9.5e-67	63.56	uncharacterized protein LOC101217202 isoform X2 [Cucumis sativus]	[more]

BLAST of MS004538 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8CFK2	9.7e-06	35.20	Transcription factor IIIB 90 kDa subunit OS=Mus musculus OX=10090 GN=Brf1 PE=1 S...	[more]
Q92994	1.3e-05	34.17	Transcription factor IIIB 90 kDa subunit OS=Homo sapiens OX=9606 GN=BRF1 PE=1 SV...	[more]
Q9P6R0	1.1e-04	29.36	Transcription factor IIIB 60 kDa subunit OS=Schizosaccharomyces pombe (strain 97...	[more]

BLAST of MS004538 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CLW7	5.0e-106	99.04	transcription factor IIIB 90 kDa subunit-like isoform X1 OS=Momordica charantia ...	[more]
A0A6J1CMP1	8.9e-87	98.85	uncharacterized protein LOC111012793 isoform X2 OS=Momordica charantia OX=3673 G...	[more]
A0A5D3E7S6	3.5e-67	68.37	Transcription factor IIIB 90 kDa subunit-like isoform X3 OS=Cucumis melo var. ma...	[more]
A0A1S3B993	3.5e-67	68.37	uncharacterized protein LOC103487591 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A0A0L0J7	4.6e-67	63.56	BRF1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G363410 PE=4 S...	[more]

BLAST of MS004538 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G09360.1	1.4e-15	33.48	Cyclin/Brf1-like TBP-binding protein	[more]
AT4G19550.1	7.6e-06	29.95	zinc ion binding;transcription regulators	[more]
AT2G01280.1	5.5e-04	32.34	Cyclin/Brf1-like TBP-binding protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.20.5.650	Single helix bin	coord: 13..76 e-value: 1.3E-15 score: 58.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 155..187
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 139..154
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 139..213
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 72..112
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 195..213
IPR011665	Brf1, TBP-binding domain	PFAM	PF07741	BRF1	coord: 35..133 e-value: 1.2E-24 score: 86.5
IPR029529	Transcription factor IIIB subunit Brf1	PANTHER	PTHR11618:SF4	TRANSCRIPTION FACTOR IIIB 90 KDA SUBUNIT	coord: 23..136
IPR000812	Transcription factor TFIIB	PANTHER	PTHR11618	TRANSCRIPTION INITIATION FACTOR IIB-RELATED	coord: 23..136

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS004538.1	MS004538.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006352	DNA-templated transcription, initiation
biological_process	GO:0006383	transcription by RNA polymerase III
biological_process	GO:0070897	transcription preinitiation complex assembly
cellular_component	GO:0000126	transcription factor TFIIIB complex
molecular_function	GO:0000995	RNA polymerase III general transcription initiation factor activity