MS004531 (gene) Bitter gourd (TR) v1

Overview


Name	MS004531
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	aspartic proteinase Asp1-like isoform X2
Location	scaffold995: 66050 .. 67924 (-)
RNA-Seq Expression	MS004531
Synteny	MS004531

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCATAGCCGGCTTCTGTTTCCTGTCTCCATTCTCTTCGCGATTTTCGCCGTTTCCTTCTCCGACAAGTTTTCCCTCGAAGACCGGATGCAATCGGGGAGTGGGAGCAATCGTTTTGCTTCGTCGGTTGCGTTTCCGATCAAAGGAAACGTTTATCCTCTAGGGTTTGTATCCTACATTCTTGCTCCATCTTTAATTTTGATTCTCTCTCTCGTCTCTCGTCTAAATTTCCTCGATTCTGGCTTCGAATCTGCAGCCATTTTACGGTATCTGTCAACATTGGCAATCCGCCGAAGATTTTTGAGCTCGATATTGACACTGGGAGTGACCTCACTTGGGTCCAATGCGATGCCCCGTGTACTGGTTGTACTCTGGTTAGTTTGATCCAAATTGATGGCTTATTCGATAGTTGCTTTCTTTTCTTCTACCGTAATTTTGTGCCATCCATGACTCTGCAGCCTCGTGATAAGCAATATAGACCACACAGCAACGCTGTGCGTTGTGCAGAACCGCTGTGTTCGGCTCTTTTTTTCCCAGGCAAGGTCCCTTGCAAGAACCCTAATGATCAATGTGACTATGATGTTGAATATGCTGACCAAGGATCTTCCATTGGCGTATTGGTCAGAGATTTAGTTCCCATGAGACTCACTAATGGCTCTGTCTTTGCTCCCAATCTGGGCTTTGGGTGTGTATATAACATATCAGTTGATTGCTTGGTTTGGCTATGCCAACAGTAATTTTTTGGTCCTTAATCTTTCTTTCCTCTGGTTCTCTTGTCTGCAGTTGTGGATATGATCAGAAGCATGGTGGTTCAACAGCTGGTGTCCTGGGCCTTGGAAGTGGTAAAGCTACCATGACATCGCAGCTAAGCGCTCTCGGTCATGTACGTAACGTAGTCGGCCACTGCCTCAGCGGACAAGGGGGAGGATTTTTATTCTTTGGAGGAGACCTTGTTCCATCTTCAGGGATGTCGTGGACGCCGATAATGCCCTCTCCTGGAGGGTCAGTGAGCGTTGAGCAATCAGACAACTGTTGTAACTGATCATTGCTTCTTAAATGTGACTGAACTTGTATTTGATCTGATTCTCAGGAGGTACTCATCTGGACCTGCAGAGGTCTATTTTGGTGGGAAGGCTGCTGGAATTAGAGGCCTTACCCTGACCTTTGACAGTGGAAGCTCCTATACTTATTTCAACTCCCAAGTCTATGGAGCAGTACTCAACTTGGTAAGATGGAATCAAGAAATCTTTTTACACTCATAAATGTGTTTTAGAAAGAAACTAATAATCTCTAATCACATCCCAGTTGAAGAATGATTTGAAAGGAAAGCCTCTGTCTGATGAACCTAAAGATAAAACCCTTCCAGTATGCTGGAAAGGCACAAAAACTTTCAGATCTGTGGTTGATGTTAGAAACTTTTTCAAGCCTCTGGCCCTGAGCTTTACCGACTCCAAGAATGTTCAGTTTCAAATACCGCCTGAAGCTTATCTAATTATCAGTGTAAGTTCCCAAACATCTCATTTATCTATATATCCATAGTTAAACTGAAACCATATATTGCGAGTATCATCACCATATCTAAGACTCTTCTTTCTTATACAGAAATTTGGTAATGTGTGCTTTGGGATTTTGAATGGGTCCCAAGTAGGATTGGGAAATGTTAATGTGATTGGAGGTAAGATGAGAAACACTGTTGGATCAACAAAGAACCACTTTCAGGTTCTTTTCTTTTACATGCTTTCTTCCAACATTTCAACTTCTTTTTCCCCCAAATTTGTTACAGATATCTCTTTGCTCGATAAGATTGTAGTCTATGACAACGAGAGACAGCAGATCGGGTGGGCACCTGCAAACTGCAACAGACCTCCCAAGAAA

mRNA sequence

ATGCATAGCCGGCTTCTGTTTCCTGTCTCCATTCTCTTCGCGATTTTCGCCGTTTCCTTCTCCGACAAGTTTTCCCTCGAAGACCGGATGCAATCGGGGAGTGGGAGCAATCGTTTTGCTTCGTCGGTTGCGTTTCCGATCAAAGGAAACGTTTATCCTCTAGGCCATTTTACGGTATCTGTCAACATTGGCAATCCGCCGAAGATTTTTGAGCTCGATATTGACACTGGGAGTGACCTCACTTGGGTCCAATGCGATGCCCCGTGTACTGGTTGTACTCTGCCTCGTGATAAGCAATATAGACCACACAGCAACGCTGTGCGTTGTGCAGAACCGCTGTGTTCGGCTCTTTTTTTCCCAGGCAAGGTCCCTTGCAAGAACCCTAATGATCAATGTGACTATGATGTTGAATATGCTGACCAAGGATCTTCCATTGGCGTATTGGTCAGAGATTTAGTTCCCATGAGACTCACTAATGGCTCTGTCTTTGCTCCCAATCTGGGCTTTGGTTGTGGATATGATCAGAAGCATGGTGGTTCAACAGCTGGTGTCCTGGGCCTTGGAAGTGGTAAAGCTACCATGACATCGCAGCTAAGCGCTCTCGGTCATGTACGTAACGTAGTCGGCCACTGCCTCAGCGGACAAGGGGGAGGATTTTTATTCTTTGGAGGAGACCTTGTTCCATCTTCAGGGATGTCGTGGACGCCGATAATGCCCTCTCCTGGAGGGAGGTACTCATCTGGACCTGCAGAGGTCTATTTTGGTGGGAAGGCTGCTGGAATTAGAGGCCTTACCCTGACCTTTGACAGTGGAAGCTCCTATACTTATTTCAACTCCCAAGTCTATGGAGCAGTACTCAACTTGTTGAAGAATGATTTGAAAGGAAAGCCTCTGTCTGATGAACCTAAAGATAAAACCCTTCCAGTATGCTGGAAAGGCACAAAAACTTTCAGATCTGTGGTTGATGTTAGAAACTTTTTCAAGCCTCTGGCCCTGAGCTTTACCGACTCCAAGAATGTTCAGTTTCAAATACCGCCTGAAGCTTATCTAATTATCAGTAAATTTGGTAATGTGTGCTTTGGGATTTTGAATGGGTCCCAAGTAGGATTGGGAAATGTTAATGTGATTGGAGATATCTCTTTGCTCGATAAGATTGTAGTCTATGACAACGAGAGACAGCAGATCGGGTGGGCACCTGCAAACTGCAACAGACCTCCCAAGAAA

Coding sequence (CDS)

ATGCATAGCCGGCTTCTGTTTCCTGTCTCCATTCTCTTCGCGATTTTCGCCGTTTCCTTCTCCGACAAGTTTTCCCTCGAAGACCGGATGCAATCGGGGAGTGGGAGCAATCGTTTTGCTTCGTCGGTTGCGTTTCCGATCAAAGGAAACGTTTATCCTCTAGGCCATTTTACGGTATCTGTCAACATTGGCAATCCGCCGAAGATTTTTGAGCTCGATATTGACACTGGGAGTGACCTCACTTGGGTCCAATGCGATGCCCCGTGTACTGGTTGTACTCTGCCTCGTGATAAGCAATATAGACCACACAGCAACGCTGTGCGTTGTGCAGAACCGCTGTGTTCGGCTCTTTTTTTCCCAGGCAAGGTCCCTTGCAAGAACCCTAATGATCAATGTGACTATGATGTTGAATATGCTGACCAAGGATCTTCCATTGGCGTATTGGTCAGAGATTTAGTTCCCATGAGACTCACTAATGGCTCTGTCTTTGCTCCCAATCTGGGCTTTGGTTGTGGATATGATCAGAAGCATGGTGGTTCAACAGCTGGTGTCCTGGGCCTTGGAAGTGGTAAAGCTACCATGACATCGCAGCTAAGCGCTCTCGGTCATGTACGTAACGTAGTCGGCCACTGCCTCAGCGGACAAGGGGGAGGATTTTTATTCTTTGGAGGAGACCTTGTTCCATCTTCAGGGATGTCGTGGACGCCGATAATGCCCTCTCCTGGAGGGAGGTACTCATCTGGACCTGCAGAGGTCTATTTTGGTGGGAAGGCTGCTGGAATTAGAGGCCTTACCCTGACCTTTGACAGTGGAAGCTCCTATACTTATTTCAACTCCCAAGTCTATGGAGCAGTACTCAACTTGTTGAAGAATGATTTGAAAGGAAAGCCTCTGTCTGATGAACCTAAAGATAAAACCCTTCCAGTATGCTGGAAAGGCACAAAAACTTTCAGATCTGTGGTTGATGTTAGAAACTTTTTCAAGCCTCTGGCCCTGAGCTTTACCGACTCCAAGAATGTTCAGTTTCAAATACCGCCTGAAGCTTATCTAATTATCAGTAAATTTGGTAATGTGTGCTTTGGGATTTTGAATGGGTCCCAAGTAGGATTGGGAAATGTTAATGTGATTGGAGATATCTCTTTGCTCGATAAGATTGTAGTCTATGACAACGAGAGACAGCAGATCGGGTGGGCACCTGCAAACTGCAACAGACCTCCCAAGAAA

Protein sequence

MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVSVNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLVPMRLTNGSVFAPNLGFGCGYDQKHGGSTAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKTFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCNRPPKK

Homology

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BLAST of MS004531 vs. NCBI nr
Match: XP_022132194.1 (aspartic proteinase Asp1-like isoform X4 [Momordica charantia])

HSP 1 Score: 842.4 bits (2175), Expect = 1.6e-240
Identity = 406/408 (99.51%), Postives = 406/408 (99.51%), Query Frame = 0


Query: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60
           MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS
Sbjct: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60

Query: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP 120
           VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP
Sbjct: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP 120

Query: 121 GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLVPMRLTNGSVFAPNLGFGCGYDQKHGGS 180
           GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL PMRLTNGSVFAPNLGFGCGYDQKHGGS
Sbjct: 121 GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGGS 180

Query: 181 TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS 240
           TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS
Sbjct: 181 TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS 240

Query: 241 PGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSD 300
           PGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSD
Sbjct: 241 PGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSD 300

Query: 301 EPKDKTLPVCWKGTKTFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFGNVCF 360
           EPKDKTLPVCWKGTK FRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFGNVCF
Sbjct: 301 EPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFGNVCF 360

Query: 361 GILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCNRPPKK 409
           GILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCNRPPKK
Sbjct: 361 GILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCNRPPKK 408

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BLAST of MS004531 vs. NCBI nr
Match: XP_022132187.1 (aspartic proteinase Asp1-like isoform X3 [Momordica charantia])

HSP 1 Score: 827.4 bits (2136), Expect = 5.4e-236
Identity = 406/436 (93.12%), Postives = 406/436 (93.12%), Query Frame = 0


Query: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60
           MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS
Sbjct: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60

Query: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTL-------------------------- 120
           VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTL                          
Sbjct: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLVSLIQIDGLFDSCFLFFYRNFVPSMT 120

Query: 121 --PRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL 180
             PRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL
Sbjct: 121 LQPRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL 180

Query: 181 VPMRLTNGSVFAPNLGFGCGYDQKHGGSTAGVLGLGSGKATMTSQLSALGHVRNVVGHCL 240
            PMRLTNGSVFAPNLGFGCGYDQKHGGSTAGVLGLGSGKATMTSQLSALGHVRNVVGHCL
Sbjct: 181 FPMRLTNGSVFAPNLGFGCGYDQKHGGSTAGVLGLGSGKATMTSQLSALGHVRNVVGHCL 240

Query: 241 SGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGS 300
           SGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGS
Sbjct: 241 SGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGS 300

Query: 301 SYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKTFRSVVDVRNFFKPLAL 360
           SYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTK FRSVVDVRNFFKPLAL
Sbjct: 301 SYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLAL 360

Query: 361 SFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIGDISLLDKIVVYDNER 409
           SFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIGDISLLDKIVVYDNER
Sbjct: 361 SFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIGDISLLDKIVVYDNER 420

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BLAST of MS004531 vs. NCBI nr
Match: XP_022132180.1 (aspartic proteinase Asp1-like isoform X2 [Momordica charantia])

HSP 1 Score: 824.3 bits (2128), Expect = 4.6e-235
Identity = 406/444 (91.44%), Postives = 406/444 (91.44%), Query Frame = 0


Query: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60
           MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS
Sbjct: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60

Query: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP 120
           VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP
Sbjct: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP 120

Query: 121 GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLVPMRLTNGSVFAPNLGFGCGYDQKHGGS 180
           GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL PMRLTNGSVFAPNLGFGCGYDQKHGGS
Sbjct: 121 GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGGS 180

Query: 181 TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS 240
           TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS
Sbjct: 181 TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS 240

Query: 241 PGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSD 300
           PGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSD
Sbjct: 241 PGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSD 300

Query: 301 EPKDKTLPVCWKGTKTFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFGNVCF 360
           EPKDKTLPVCWKGTK FRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFGNVCF
Sbjct: 301 EPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFGNVCF 360

Query: 361 GILNGSQVGLGNVNVIG------------------------------------DISLLDK 409
           GILNGSQVGLGNVNVIG                                    DISLLDK
Sbjct: 361 GILNGSQVGLGNVNVIGGKMRNTVGSTKNHFQVLFFYMLSSNISTSFSPKFVTDISLLDK 420

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BLAST of MS004531 vs. NCBI nr
Match: XP_022132155.1 (aspartic proteinase Asp1-like isoform X1 [Momordica charantia] >XP_022132164.1 aspartic proteinase Asp1-like isoform X1 [Momordica charantia] >XP_022132172.1 aspartic proteinase Asp1-like isoform X1 [Momordica charantia])

HSP 1 Score: 809.3 bits (2089), Expect = 1.5e-230
Identity = 406/472 (86.02%), Postives = 406/472 (86.02%), Query Frame = 0


Query: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60
           MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS
Sbjct: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60

Query: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTL-------------------------- 120
           VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTL                          
Sbjct: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLVSLIQIDGLFDSCFLFFYRNFVPSMT 120

Query: 121 --PRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL 180
             PRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL
Sbjct: 121 LQPRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL 180

Query: 181 VPMRLTNGSVFAPNLGFGCGYDQKHGGSTAGVLGLGSGKATMTSQLSALGHVRNVVGHCL 240
            PMRLTNGSVFAPNLGFGCGYDQKHGGSTAGVLGLGSGKATMTSQLSALGHVRNVVGHCL
Sbjct: 181 FPMRLTNGSVFAPNLGFGCGYDQKHGGSTAGVLGLGSGKATMTSQLSALGHVRNVVGHCL 240

Query: 241 SGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGS 300
           SGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGS
Sbjct: 241 SGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGS 300

Query: 301 SYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKTFRSVVDVRNFFKPLAL 360
           SYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTK FRSVVDVRNFFKPLAL
Sbjct: 301 SYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLAL 360

Query: 361 SFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIG--------------- 409
           SFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIG               
Sbjct: 361 SFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIGGKMRNTVGSTKNHFQ 420

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BLAST of MS004531 vs. NCBI nr
Match: XP_004142705.1 (aspartic proteinase Asp1 [Cucumis sativus])

HSP 1 Score: 666.4 bits (1718), Expect = 1.6e-187
Identity = 317/413 (76.76%), Postives = 355/413 (85.96%), Query Frame = 0


Query: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60
           MH+  L P+S+LF IF++SF   F L D     S  N F SS+  P+KGNVYPLGHFTVS
Sbjct: 1   MHAFNLSPLSLLFLIFSLSFCGTFCLAD--WKSSAVNPFDSSILLPVKGNVYPLGHFTVS 60

Query: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP 120
           V IGNPPK+FELDIDTGSDLTWVQCDAPCTGCTLP D+ Y+PH+N VRC EPLCSALF  
Sbjct: 61  VTIGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLPHDRLYKPHNNVVRCGEPLCSALFSA 120

Query: 121 GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLVPMRLTNGSVFAPNLGFGCGYDQKHGGS 180
            K PCKNPNDQCDY+VEYAD GSSIGVLV+D VP+RLTNG++ APNLGFGCGYDQ +GGS
Sbjct: 121 SKSPCKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNGTILAPNLGFGCGYDQHNGGS 180

Query: 181 -----TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWT 240
                TAGVLGLG+ KATM +QLSAL HVRNV+GHC SGQGGGFLFFGGDLVPSSGMSW 
Sbjct: 181 QLPPLTAGVLGLGNSKATMATQLSALSHVRNVLGHCFSGQGGGFLFFGGDLVPSSGMSWM 240

Query: 241 PIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKG 300
           PI+ +PGG+YS+GPAEVYFGG   GIRGL LTFDSGSSYTYFNSQVYGAVLNLL+N LKG
Sbjct: 241 PILRTPGGKYSAGPAEVYFGGNPVGIRGLILTFDSGSSYTYFNSQVYGAVLNLLRNGLKG 300

Query: 301 KPLSDEPKDKTLPVCWKGTKTFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKF 360
           +PL D P+DKTLP+CWKG+K F+SV DVRNFFKPLALSF +SK VQFQIPPEAYLIIS  
Sbjct: 301 QPLRDAPEDKTLPICWKGSKAFKSVADVRNFFKPLALSFGNSK-VQFQIPPEAYLIISNL 360

Query: 361 GNVCFGILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCNRPPKK 409
           GNVC GILNGSQVGLGNVN+IGDIS+LDK++VYDNERQQIGWAPANC++PP+K
Sbjct: 361 GNVCLGILNGSQVGLGNVNLIGDISMLDKMMVYDNERQQIGWAPANCSKPPRK 410

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BLAST of MS004531 vs. ExPASy Swiss-Prot
Match: Q0IU52 (Aspartic proteinase Asp1 OS=Oryza sativa subsp. japonica OX=39947 GN=ASP1 PE=2 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 6.1e-81
Identity = 167/384 (43.49%), Postives = 237/384 (61.72%), Query Frame = 0


Query: 40  ASSVAFPIKGNVYPLGHFTVSVNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQ 99
           +S+V   + GNVYP+GHF +++NIG+P K + LDIDTGS LTW+QCDAPCT C +     
Sbjct: 21  SSAVVLELHGNVYPIGHFFITMNIGDPAKSYFLDIDTGSTLTWLQCDAPCTNCNIVPHVL 80

Query: 100 YRP-HSNAVRCAEPLCSALFFP-GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLVPMRL 159
           Y+P     V CA+ LC+ L+   GK        QCDY ++Y D  SS+GVLV D   +  
Sbjct: 81  YKPTPKKLVTCADSLCTDLYTDLGKPKRCGSQKQCDYVIQYVD-SSSMGVLVIDRFSLSA 140

Query: 160 TNGSVFAPNLGFGCGYDQKHGGSTA-----GVLGLGSGKATMTSQLSALGHV-RNVVGHC 219
           +NG+     + FGCGYDQ             +LGL  GK T+ SQL + G + ++V+GHC
Sbjct: 141 SNGT-NPTTIAFGCGYDQGKKNRNVPIPVDSILGLSRGKVTLLSQLKSQGVITKHVLGHC 200

Query: 220 LSGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYF--GGKAAGIRGLTLTFD 279
           +S +GGGFLFFG   VP+SG++WTP M      YS G   ++F    KA     + + FD
Sbjct: 201 ISSKGGGFLFFGDAQVPTSGVTWTP-MNREHKYYSPGHGTLHFDSNSKAISAAPMAVIFD 260

Query: 280 SGSSYTYFNSQVYGAVLNLLKNDLKG--KPLSD-EPKDKTLPVCWKGTKTFRSVVDVRNF 339
           SG++YTYF +Q Y A L+++K+ L    K L++   KD+ L VCWKG     ++ +V+  
Sbjct: 261 SGATYTYFAAQPYQATLSVVKSTLNSECKFLTEVTEKDRALTVCWKGKDKIVTIDEVKKC 320

Query: 340 FKPLALSFTD-SKNVQFQIPPEAYLIISKFGNVCFGILNGSQ--VGLGNVNVIGDISLLD 399
           F+ L+L F D  K    +IPPE YLIIS+ G+VC GIL+GS+  + L   N+IG I++LD
Sbjct: 321 FRSLSLEFADGDKKATLEIPPEHYLIISQEGHVCLGILDGSKEHLSLAGTNLIGGITMLD 380

Query: 400 KIVVYDNERQQIGWAPANCNRPPK 408
           ++V+YD+ER  +GW    C+R P+
Sbjct: 381 QMVIYDSERSLLGWVNYQCDRIPR 401

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BLAST of MS004531 vs. ExPASy Swiss-Prot
Match: A2ZC67 (Aspartic proteinase Asp1 OS=Oryza sativa subsp. indica OX=39946 GN=ASP1 PE=2 SV=2)

HSP 1 Score: 296.6 bits (758), Expect = 4.3e-79
Identity = 166/384 (43.23%), Postives = 239/384 (62.24%), Query Frame = 0


Query: 40  ASSVAFPIKGNVYPLGHFTVSVNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQ 99
           +S+V   + GNVYP+GHF V++NIG+P K + LDIDTGS LTW+QCD PC  C       
Sbjct: 21  SSAVVLELHGNVYPIGHFFVTMNIGDPAKPYFLDIDTGSTLTWLQCDYPCINCNKVPHGL 80

Query: 100 YRPH-SNAVRCAEPLCSALFFPGKVPCK-NPNDQCDYDVEYADQGSSIGVLVRDLVPMRL 159
           Y+P    AV+C E  C+ L+   + P K  P +QC Y ++Y   GSSIGVL+ D   +  
Sbjct: 81  YKPELKYAVKCTEQRCADLYADLRKPMKCGPKNQCHYGIQYVG-GSSIGVLIVDSFSLPA 140

Query: 160 TNGSVFAPNLGFGCGYDQ---KHGGSTA--GVLGLGSGKATMTSQLSALGHV-RNVVGHC 219
           +NG+    ++ FGCGY+Q    H   T   G+LGLG GK T+ SQL + G + ++V+GHC
Sbjct: 141 SNGT-NPTSIAFGCGYNQGKNNHNVPTPVNGILGLGRGKVTLLSQLKSQGVITKHVLGHC 200

Query: 220 LSGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRG--LTLTFD 279
           +S +G GFLFFG   VP+SG++W+P M      YS     + F   +  I    + + FD
Sbjct: 201 ISSKGKGFLFFGDAKVPTSGVTWSP-MNREHKHYSPRQGTLQFNSNSKPISAAPMEVIFD 260

Query: 280 SGSSYTYFNSQVYGAVLNLLKNDL--KGKPLSD-EPKDKTLPVCWKGTKTFRSVVDVRNF 339
           SG++YTYF  Q Y A L+++K+ L  + K L++ + KD+ L VCWKG    R++ +V+  
Sbjct: 261 SGATYTYFALQPYHATLSVVKSTLSKECKFLTEVKEKDRALTVCWKGKDKIRTIDEVKKC 320

Query: 340 FKPLALSFTD-SKNVQFQIPPEAYLIISKFGNVCFGILNGSQ--VGLGNVNVIGDISLLD 399
           F+ L+L F D  K    +IPPE YLIIS+ G+VC GIL+GS+    L   N+IG I++LD
Sbjct: 321 FRSLSLKFADGDKKATLEIPPEHYLIISQEGHVCLGILDGSKEHPSLAGTNLIGGITMLD 380

Query: 400 KIVVYDNERQQIGWAPANCNRPPK 408
           ++V+YD+ER  +GW    C+R P+
Sbjct: 381 QMVIYDSERSLLGWVNYQCDRIPR 401

[Close]

BLAST of MS004531 vs. ExPASy Swiss-Prot
Match: Q9M9A8 (Aspartyl protease APCB1 OS=Arabidopsis thaliana OX=3702 GN=APCB1 PE=1 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 7.7e-76
Identity = 160/398 (40.20%), Postives = 235/398 (59.05%), Query Frame = 0


Query: 32  SGSGSNRFASSVAFPIKGNVYPLGHFTVSVNIGNPP--KIFELDIDTGSDLTWVQCDAPC 91
           S S  +  +S+  FP+ GNVYP G +   + +G P   + + LDIDTGS+LTW+QCDAPC
Sbjct: 178 STSAGSIDSSTTIFPVGGNVYPDGLYYTRILVGKPEDGQYYHLDIDTGSELTWIQCDAPC 237

Query: 92  TGCTLPRDKQYRPH-SNAVRCAEPLCSALFFPGKVP-CKNPNDQCDYDVEYADQGSSIGV 151
           T C    ++ Y+P   N VR +E  C  +        C+N + QCDY++EYAD   S+GV
Sbjct: 238 TSCAKGANQLYKPRKDNLVRSSEAFCVEVQRNQLTEHCENCH-QCDYEIEYADHSYSMGV 297

Query: 152 LVRDLVPMRLTNGSVFAPNLGFGCGYDQKHGG-------STAGVLGLGSGKATMTSQLSA 211
           L +D   ++L NGS+   ++ FGCGYDQ+  G        T G+LGL   K ++ SQL++
Sbjct: 298 LTKDKFHLKLHNGSLAESDIVFGCGYDQQ--GLLLNTLLKTDGILGLSRAKISLPSQLAS 357

Query: 212 LGHVRNVVGHCLSG--QGGGFLFFGGDLVPSSGMSWTPIM-PSPGGRYSSGPAEVYFGGK 271
            G + NVVGHCL+    G G++F G DLVPS GM+W P++  S    Y     ++ +G  
Sbjct: 358 RGIISNVVGHCLASDLNGEGYIFMGSDLVPSHGMTWVPMLHDSRLDAYQMQVTKMSYGQG 417

Query: 272 AAGIRGLT-----LTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWK 331
              + G       + FD+GSSYTYF +Q Y  ++  L+ ++ G  L+ +  D+TLP+CW+
Sbjct: 418 MLSLDGENGRVGKVLFDTGSSYTYFPNQAYSQLVTSLQ-EVSGLELTRDDSDETLPICWR 477

Query: 332 GTKT--FRSVVDVRNFFKPLALSFTDSKNV---QFQIPPEAYLIISKFGNVCFGILNGSQ 391
                 F S+ DV+ FF+P+ L       +   +  I PE YLIIS  GNVC GIL+GS 
Sbjct: 478 AKTNFPFSSLSDVKKFFRPITLQIGSKWLIISRKLLIQPEDYLIISNKGNVCLGILDGSS 537

Query: 392 VGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCNRP 406
           V  G+  ++GDIS+   ++VYDN +++IGW  ++C RP
Sbjct: 538 VHDGSTIILGDISMRGHLIVYDNVKRRIGWMKSDCVRP 571

[Close]

BLAST of MS004531 vs. ExPASy Swiss-Prot
Match: Q9S9K4 (Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2)

HSP 1 Score: 146.7 bits (369), Expect = 5.6e-34
Identity = 114/394 (28.93%), Postives = 182/394 (46.19%), Query Frame = 0


Query: 37  NRFASSVAFPIKGN--VYPLGHFTVSVNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTL 96
           +R  +S+  P+ G+  V  +G +   + +G+PPK + + +DTGSD+ W+ C  PC  C  
Sbjct: 52  SRMLASIDLPLGGDSRVDSVGLYFTKIKLGSPPKEYHVQVDTGSDILWINC-KPCPKCPT 111

Query: 97  PRDKQYR---------PHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSI 156
             +  +R           S  V C +  CS  F      C+ P   C Y + YAD+ +S 
Sbjct: 112 KTNLNFRLSLFDMNASSTSKKVGCDDDFCS--FISQSDSCQ-PALGCSYHIVYADESTSD 171

Query: 157 GVLVRDLVPMRLTNGSVFAPNLG----FGCGYDQK----HGGSTA-GVLGLGSGKATMTS 216
           G  +RD++ +    G +    LG    FGCG DQ     +G S   GV+G G    ++ S
Sbjct: 172 GKFIRDMLTLEQVTGDLKTGPLGQEVVFGCGSDQSGQLGNGDSAVDGVMGFGQSNTSVLS 231

Query: 217 QLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGG 276
           QL+A G  + V  HCL    GG +F  G +V S  +  TP++P+    Y+     +   G
Sbjct: 232 QLAATGDAKRVFSHCLDNVKGGGIFAVG-VVDSPKVKTTPMVPNQ-MHYNVMLMGMDVDG 291

Query: 277 KAAG-----IRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCW 336
            +       +R      DSG++  YF   +Y    +L++  L  +P+     ++T     
Sbjct: 292 TSLDLPRSIVRNGGTIVDSGTTLAYFPKVLYD---SLIETILARQPVKLHIVEETF---- 351

Query: 337 KGTKTFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQV--G 396
              + F    +V   F P++  F DS  V+  + P  YL   +    CFG   G      
Sbjct: 352 ---QCFSFSTNVDEAFPPVSFEFEDS--VKLTVYPHDYLFTLEEELYCFGWQAGGLTTDE 411

Query: 397 LGNVNVIGDISLLDKIVVYDNERQQIGWAPANCN 404
              V ++GD+ L +K+VVYD + + IGWA  NC+
Sbjct: 412 RSEVILLGDLVLSNKLVVYDLDNEVIGWADHNCS 427

[Close]

BLAST of MS004531 vs. ExPASy Swiss-Prot
Match: Q4V3D2 (Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 2.6e-31
Identity = 110/431 (25.52%), Postives = 188/431 (43.62%), Query Frame = 0


Query: 9   VSILFAIFAVSFSDKFSLEDRMQSGSGSN------RFASSVAFPIKGN--VYPLGHFTVS 68
           + ++   F  + + KF+ +++  S   S+      R  +++  P+ G+     +G +   
Sbjct: 22  IQVVSGNFVFNVTHKFAGKEKQLSELKSHDSFRHARMLANIDLPLGGDSRADSIGLYFTK 81

Query: 69  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPR---------DKQYRPHSNAVRCAE 128
           + +G+PPK + + +DTGSD+ WV C APC  C +           D +    S  V C +
Sbjct: 82  IKLGSPPKEYYVQVDTGSDILWVNC-APCPKCPVKTDLGIPLSLYDSKTSSTSKNVGCED 141

Query: 129 PLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLVPMRLTNGSV----FAPNL 188
             CS +        K P   C Y V Y D  +S G  ++D + +    G++     A  +
Sbjct: 142 DFCSFIMQSETCGAKKP---CSYHVVYGDGSTSDGDFIKDNITLEQVTGNLRTAPLAQEV 201

Query: 189 GFGCGYDQ-----KHGGSTAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFF 248
            FGCG +Q     +   +  G++G G    ++ SQL+A G  + +  HCL    GG +F 
Sbjct: 202 VFGCGKNQSGQLGQTDSAVDGIMGFGQSNTSIISQLAAGGSTKRIFSHCLDNMNGGGIFA 261

Query: 249 GGDLVPSSGMSWTPIMPS--------PGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSY 308
            G+ V S  +  TPI+P+         G      P ++     +    G T+  DSG++ 
Sbjct: 262 VGE-VESPVVKTTPIVPNQVHYNVILKGMDVDGDPIDLPPSLASTNGDGGTI-IDSGTTL 321

Query: 309 TYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKTFRSVVDVRNFFKPLALSF 368
            Y    +Y +++  +    + K          L +  +    F    +    F  + L F
Sbjct: 322 AYLPQNLYNSLIEKITAKQQVK----------LHMVQETFACFSFTSNTDKAFPVVNLHF 381

Query: 369 TDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVI--GDISLLDKIVVYDNER 404
            DS  ++  + P  YL   +    CFG  +G        +VI  GD+ L +K+VVYD E 
Sbjct: 382 EDS--LKLSVYPHDYLFSLREDMYCFGWQSGGMTTQDGADVILLGDLVLSNKLVVYDLEN 434

[Close]

BLAST of MS004531 vs. ExPASy TrEMBL
Match: A0A6J1BSD8 (aspartic proteinase Asp1-like isoform X4 OS=Momordica charantia OX=3673 GN=LOC111005085 PE=3 SV=1)

HSP 1 Score: 842.4 bits (2175), Expect = 7.8e-241
Identity = 406/408 (99.51%), Postives = 406/408 (99.51%), Query Frame = 0


Query: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60
           MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS
Sbjct: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60

Query: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP 120
           VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP
Sbjct: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP 120

Query: 121 GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLVPMRLTNGSVFAPNLGFGCGYDQKHGGS 180
           GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL PMRLTNGSVFAPNLGFGCGYDQKHGGS
Sbjct: 121 GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGGS 180

Query: 181 TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS 240
           TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS
Sbjct: 181 TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS 240

Query: 241 PGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSD 300
           PGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSD
Sbjct: 241 PGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSD 300

Query: 301 EPKDKTLPVCWKGTKTFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFGNVCF 360
           EPKDKTLPVCWKGTK FRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFGNVCF
Sbjct: 301 EPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFGNVCF 360

Query: 361 GILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCNRPPKK 409
           GILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCNRPPKK
Sbjct: 361 GILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCNRPPKK 408

[Close]

BLAST of MS004531 vs. ExPASy TrEMBL
Match: A0A6J1BT55 (aspartic proteinase Asp1-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC111005085 PE=3 SV=1)

HSP 1 Score: 827.4 bits (2136), Expect = 2.6e-236
Identity = 406/436 (93.12%), Postives = 406/436 (93.12%), Query Frame = 0


Query: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60
           MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS
Sbjct: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60

Query: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTL-------------------------- 120
           VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTL                          
Sbjct: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLVSLIQIDGLFDSCFLFFYRNFVPSMT 120

Query: 121 --PRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL 180
             PRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL
Sbjct: 121 LQPRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL 180

Query: 181 VPMRLTNGSVFAPNLGFGCGYDQKHGGSTAGVLGLGSGKATMTSQLSALGHVRNVVGHCL 240
            PMRLTNGSVFAPNLGFGCGYDQKHGGSTAGVLGLGSGKATMTSQLSALGHVRNVVGHCL
Sbjct: 181 FPMRLTNGSVFAPNLGFGCGYDQKHGGSTAGVLGLGSGKATMTSQLSALGHVRNVVGHCL 240

Query: 241 SGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGS 300
           SGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGS
Sbjct: 241 SGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGS 300

Query: 301 SYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKTFRSVVDVRNFFKPLAL 360
           SYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTK FRSVVDVRNFFKPLAL
Sbjct: 301 SYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLAL 360

Query: 361 SFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIGDISLLDKIVVYDNER 409
           SFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIGDISLLDKIVVYDNER
Sbjct: 361 SFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIGDISLLDKIVVYDNER 420

[Close]

BLAST of MS004531 vs. ExPASy TrEMBL
Match: A0A6J1BRQ3 (aspartic proteinase Asp1-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111005085 PE=3 SV=1)

HSP 1 Score: 824.3 bits (2128), Expect = 2.2e-235
Identity = 406/444 (91.44%), Postives = 406/444 (91.44%), Query Frame = 0


Query: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60
           MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS
Sbjct: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60

Query: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP 120
           VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP
Sbjct: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP 120

Query: 121 GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLVPMRLTNGSVFAPNLGFGCGYDQKHGGS 180
           GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL PMRLTNGSVFAPNLGFGCGYDQKHGGS
Sbjct: 121 GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLFPMRLTNGSVFAPNLGFGCGYDQKHGGS 180

Query: 181 TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS 240
           TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS
Sbjct: 181 TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS 240

Query: 241 PGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSD 300
           PGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSD
Sbjct: 241 PGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLSD 300

Query: 301 EPKDKTLPVCWKGTKTFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFGNVCF 360
           EPKDKTLPVCWKGTK FRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFGNVCF
Sbjct: 301 EPKDKTLPVCWKGTKAFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKFGNVCF 360

Query: 361 GILNGSQVGLGNVNVIG------------------------------------DISLLDK 409
           GILNGSQVGLGNVNVIG                                    DISLLDK
Sbjct: 361 GILNGSQVGLGNVNVIGGKMRNTVGSTKNHFQVLFFYMLSSNISTSFSPKFVTDISLLDK 420

[Close]

BLAST of MS004531 vs. ExPASy TrEMBL
Match: A0A6J1BT39 (aspartic proteinase Asp1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005085 PE=3 SV=1)

HSP 1 Score: 809.3 bits (2089), Expect = 7.4e-231
Identity = 406/472 (86.02%), Postives = 406/472 (86.02%), Query Frame = 0


Query: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60
           MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS
Sbjct: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60

Query: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTL-------------------------- 120
           VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTL                          
Sbjct: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLVSLIQIDGLFDSCFLFFYRNFVPSMT 120

Query: 121 --PRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL 180
             PRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL
Sbjct: 121 LQPRDKQYRPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDL 180

Query: 181 VPMRLTNGSVFAPNLGFGCGYDQKHGGSTAGVLGLGSGKATMTSQLSALGHVRNVVGHCL 240
            PMRLTNGSVFAPNLGFGCGYDQKHGGSTAGVLGLGSGKATMTSQLSALGHVRNVVGHCL
Sbjct: 181 FPMRLTNGSVFAPNLGFGCGYDQKHGGSTAGVLGLGSGKATMTSQLSALGHVRNVVGHCL 240

Query: 241 SGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGS 300
           SGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGS
Sbjct: 241 SGQGGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGS 300

Query: 301 SYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKTFRSVVDVRNFFKPLAL 360
           SYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTK FRSVVDVRNFFKPLAL
Sbjct: 301 SYTYFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKAFRSVVDVRNFFKPLAL 360

Query: 361 SFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIG--------------- 409
           SFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIG               
Sbjct: 361 SFTDSKNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIGGKMRNTVGSTKNHFQ 420

[Close]

BLAST of MS004531 vs. ExPASy TrEMBL
Match: A0A0A0L366 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G361850 PE=3 SV=1)

HSP 1 Score: 666.4 bits (1718), Expect = 7.7e-188
Identity = 317/413 (76.76%), Postives = 355/413 (85.96%), Query Frame = 0


Query: 1   MHSRLLFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVS 60
           MH+  L P+S+LF IF++SF   F L D     S  N F SS+  P+KGNVYPLGHFTVS
Sbjct: 1   MHAFNLSPLSLLFLIFSLSFCGTFCLAD--WKSSAVNPFDSSILLPVKGNVYPLGHFTVS 60

Query: 61  VNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFP 120
           V IGNPPK+FELDIDTGSDLTWVQCDAPCTGCTLP D+ Y+PH+N VRC EPLCSALF  
Sbjct: 61  VTIGNPPKVFELDIDTGSDLTWVQCDAPCTGCTLPHDRLYKPHNNVVRCGEPLCSALFSA 120

Query: 121 GKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLVPMRLTNGSVFAPNLGFGCGYDQKHGGS 180
            K PCKNPNDQCDY+VEYAD GSSIGVLV+D VP+RLTNG++ APNLGFGCGYDQ +GGS
Sbjct: 121 SKSPCKNPNDQCDYEVEYADHGSSIGVLVKDPVPLRLTNGTILAPNLGFGCGYDQHNGGS 180

Query: 181 -----TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWT 240
                TAGVLGLG+ KATM +QLSAL HVRNV+GHC SGQGGGFLFFGGDLVPSSGMSW 
Sbjct: 181 QLPPLTAGVLGLGNSKATMATQLSALSHVRNVLGHCFSGQGGGFLFFGGDLVPSSGMSWM 240

Query: 241 PIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKG 300
           PI+ +PGG+YS+GPAEVYFGG   GIRGL LTFDSGSSYTYFNSQVYGAVLNLL+N LKG
Sbjct: 241 PILRTPGGKYSAGPAEVYFGGNPVGIRGLILTFDSGSSYTYFNSQVYGAVLNLLRNGLKG 300

Query: 301 KPLSDEPKDKTLPVCWKGTKTFRSVVDVRNFFKPLALSFTDSKNVQFQIPPEAYLIISKF 360
           +PL D P+DKTLP+CWKG+K F+SV DVRNFFKPLALSF +SK VQFQIPPEAYLIIS  
Sbjct: 301 QPLRDAPEDKTLPICWKGSKAFKSVADVRNFFKPLALSFGNSK-VQFQIPPEAYLIISNL 360

Query: 361 GNVCFGILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCNRPPKK 409
           GNVC GILNGSQVGLGNVN+IGDIS+LDK++VYDNERQQIGWAPANC++PP+K
Sbjct: 361 GNVCLGILNGSQVGLGNVNLIGDISMLDKMMVYDNERQQIGWAPANCSKPPRK 410

[Close]

BLAST of MS004531 vs. TAIR 10
Match: AT1G44130.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 474.2 bits (1219), Expect = 1.1e-133
Identity = 214/373 (57.37%), Postives = 283/373 (75.87%), Query Frame = 0


Query: 41  SSVAFPIKGNVYPLGHFTVSVNIGNPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQY 100
           SSV FP+ GNV+PLG+++V + IG+PPK F+ DIDTGSDLTWVQCDAPC+GCTLP + QY
Sbjct: 33  SSVVFPLSGNVFPLGYYSVLMQIGSPPKAFQFDIDTGSDLTWVQCDAPCSGCTLPPNLQY 92

Query: 101 RPHSNAVRCAEPLCSALFFPGKVPCKNPNDQCDYDVEYADQGSSIGVLVRDLVPMRLTNG 160
           +P  N + C+ P+C+AL +P K  C NP +QCDY+V+YADQGSS+G LV D  P++L NG
Sbjct: 93  KPKGNIIPCSNPICTALHWPNKPHCPNPQEQCDYEVKYADQGSSMGALVTDQFPLKLVNG 152

Query: 161 SVFAPNLGFGCGYDQKHGG-----STAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQ 220
           S   P + FGCGYDQ +       +TAGVLGLG GK  + +QL + G  RNVVGHCLS +
Sbjct: 153 SFMQPPVAFGCGYDQSYPSAHPPPATAGVLGLGRGKIGLLTQLVSAGLTRNVVGHCLSSK 212

Query: 221 GGGFLFFGGDLVPSSGMSWTPIMPSPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYT 280
           GGGFLFFG +LVPS G++WTP++ S    Y++GPA++ F GK  G++GL L FD+GSSYT
Sbjct: 213 GGGFLFFGDNLVPSIGVAWTPLL-SQDNHYTTGPADLLFNGKPTGLKGLKLIFDTGSSYT 272

Query: 281 YFNSQVYGAVLNLLKNDLKGKPLSDEPKDKTLPVCWKGTKTFRSVVDVRNFFKPLALSFT 340
           YFNS+ Y  ++NL+ NDLK  PL    +DKTLP+CWKG K F+SV++V+NFFK + ++FT
Sbjct: 273 YFNSKAYQTIINLIGNDLKVSPLKVAKEDKTLPICWKGAKPFKSVLEVKNFFKTITINFT 332

Query: 341 DS-KNVQFQIPPEAYLIISKFGNVCFGILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQ 400
           +  +N Q  + PE YLI+SK GNVC G+LNGS+VGL N NVIGDIS+   +++YDNE+QQ
Sbjct: 333 NGRRNTQLYLAPELYLIVSKTGNVCLGLLNGSEVGLQNSNVIGDISMQGLMMIYDNEKQQ 392

Query: 401 IGWAPANCNRPPK 408
           +GW  ++CN+ PK
Sbjct: 393 LGWVSSDCNKLPK 404

[Close]

BLAST of MS004531 vs. TAIR 10
Match: AT1G77480.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 454.9 bits (1169), Expect = 6.8e-128
Identity = 217/409 (53.06%), Postives = 290/409 (70.90%), Query Frame = 0


Query: 6   LFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVSVNIGN 65
           +F V IL A F  S + K   +   Q    + R +S+V FP+ GNVYPLG++ V +NIGN
Sbjct: 19  VFYVFILCARFQTSEATK---DSSAQVKLQNRRLSSTVVFPVSGNVYPLGYYYVLLNIGN 78

Query: 66  PPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFPGKVPC 125
           PPK+F+LDIDTGSDLTWVQCDAPC GCT PR KQY+P+ N + C+  LCS L  P   PC
Sbjct: 79  PPKLFDLDIDTGSDLTWVQCDAPCNGCTKPRAKQYKPNHNTLPCSHILCSGLDLPQDRPC 138

Query: 126 KNPNDQCDYDVEYADQGSSIGVLVRDLVPMRLTNGSVFAPNLGFGCGYDQKHGG-----S 185
            +P DQCDY++ Y+D  SSIG LV D VP++L NGS+    L FGCGYDQ++ G      
Sbjct: 139 ADPEDQCDYEIGYSDHASSIGALVTDEVPLKLANGSIMNLRLTFGCGYDQQNPGPHPPPP 198

Query: 186 TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMP- 245
           TAG+LGLG GK  +++QL +LG  +NV+ HCLS  G GFL  G +LVPSSG++WT +   
Sbjct: 199 TAGILGLGRGKVGLSTQLKSLGITKNVIVHCLSHTGKGFLSIGDELVPSSGVTWTSLATN 258

Query: 246 SPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLS 305
           SP   Y +GPAE+ F  K  G++G+ + FDSGSSYTYFN++ Y A+L+L++ DL GKPL+
Sbjct: 259 SPSKNYMAGPAELLFNDKTTGVKGINVVFDSGSSYTYFNAEAYQAILDLIRKDLNGKPLT 318

Query: 306 DEPKDKTLPVCWKGTKTFRSVVDVRNFFKPLALSFTDSKNVQ-FQIPPEAYLIISKFGNV 365
           D   DK+LPVCWKG K  +S+ +V+ +FK + L F + KN Q FQ+PPE+YLII++ G V
Sbjct: 319 DTKDDKSLPVCWKGKKPLKSLDEVKKYFKTITLRFGNQKNGQLFQVPPESYLIITEKGRV 378

Query: 366 CFGILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCNRPPK 408
           C GILNG+++GL   N+IGDIS    +V+YDNE+Q+IGW  ++C++ PK
Sbjct: 379 CLGILNGTEIGLEGYNIIGDISFQGIMVIYDNEKQRIGWISSDCDKLPK 424

[Close]

BLAST of MS004531 vs. TAIR 10
Match: AT1G77480.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 453.0 bits (1164), Expect = 2.6e-127
Identity = 216/408 (52.94%), Postives = 289/408 (70.83%), Query Frame = 0


Query: 6   LFPVSILFAIFAVSFSDKFSLEDRMQSGSGSNRFASSVAFPIKGNVYPLGHFTVSVNIGN 65
           +F V IL A F  S + K   +   Q    + R +S+V FP+ GNVYPLG++ V +NIGN
Sbjct: 19  VFYVFILCARFQTSEATK---DSSAQVKLQNRRLSSTVVFPVSGNVYPLGYYYVLLNIGN 78

Query: 66  PPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFPGKVPC 125
           PPK+F+LDIDTGSDLTWVQCDAPC GCT PR KQY+P+ N + C+  LCS L  P   PC
Sbjct: 79  PPKLFDLDIDTGSDLTWVQCDAPCNGCTKPRAKQYKPNHNTLPCSHILCSGLDLPQDRPC 138

Query: 126 KNPNDQCDYDVEYADQGSSIGVLVRDLVPMRLTNGSVFAPNLGFGCGYDQKHGG-----S 185
            +P DQCDY++ Y+D  SSIG LV D VP++L NGS+    L FGCGYDQ++ G      
Sbjct: 139 ADPEDQCDYEIGYSDHASSIGALVTDEVPLKLANGSIMNLRLTFGCGYDQQNPGPHPPPP 198

Query: 186 TAGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMP- 245
           TAG+LGLG GK  +++QL +LG  +NV+ HCLS  G GFL  G +LVPSSG++WT +   
Sbjct: 199 TAGILGLGRGKVGLSTQLKSLGITKNVIVHCLSHTGKGFLSIGDELVPSSGVTWTSLATN 258

Query: 246 SPGGRYSSGPAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLS 305
           SP   Y +GPAE+ F  K  G++G+ + FDSGSSYTYFN++ Y A+L+L++ DL GKPL+
Sbjct: 259 SPSKNYMAGPAELLFNDKTTGVKGINVVFDSGSSYTYFNAEAYQAILDLIRKDLNGKPLT 318

Query: 306 DEPKDKTLPVCWKGTKTFRSVVDVRNFFKPLALSFTDSKNVQ-FQIPPEAYLIISKFGNV 365
           D   DK+LPVCWKG K  +S+ +V+ +FK + L F + KN Q FQ+PPE+YLII++ G V
Sbjct: 319 DTKDDKSLPVCWKGKKPLKSLDEVKKYFKTITLRFGNQKNGQLFQVPPESYLIITEKGRV 378

Query: 366 CFGILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCNRPP 407
           C GILNG+++GL   N+IGDIS    +V+YDNE+Q+IGW  ++C++ P
Sbjct: 379 CLGILNGTEIGLEGYNIIGDISFQGIMVIYDNEKQRIGWISSDCDKLP 423

[Close]

BLAST of MS004531 vs. TAIR 10
Match: AT4G33490.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 421.8 bits (1083), Expect = 6.4e-118
Identity = 207/406 (50.99%), Postives = 267/406 (65.76%), Query Frame = 0


Query: 7   FPVSILFAIFAVSFSDKFSLEDRMQSGSGS--NRFASSVAFPIKGNVYPLGHFTVSVNIG 66
           F + ++     + FS       R  +G      R  SSV FP+ GNVYPLG++ V++NIG
Sbjct: 8   FMIVLMVMSLVLGFSSAVDFRWRKTAGFSDRFTRAVSSVVFPVHGNVYPLGYYNVTINIG 67

Query: 67  NPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFPGKVP 126
            PP+ + LD+DTGSDLTW+QCDAPC  C       Y+P S+ + C +PLC AL       
Sbjct: 68  QPPRPYYLDLDTGSDLTWLQCDAPCVRCLEAPHPLYQPSSDLIPCNDPLCKALHLNSNQR 127

Query: 127 CKNPNDQCDYDVEYADQGSSIGVLVRDLVPMRLTNGSVFAPNLGFGCGYDQKHGGST--- 186
           C+ P +QCDY+VEYAD GSS+GVLVRD+  M  T G    P L  GCGYDQ  G S+   
Sbjct: 128 CETP-EQCDYEVEYADGGSSLGVLVRDVFSMNYTQGLRLTPRLALGCGYDQIPGASSHHP 187

Query: 187 -AGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS 246
             GVLGLG GK ++ SQL + G+V+NV+GHCLS  GGG LFFG DL  SS +SWTP+   
Sbjct: 188 LDGVLGLGRGKVSILSQLHSQGYVKNVIGHCLSSLGGGILFFGDDLYDSSRVSWTPMSRE 247

Query: 247 PGGRYSSG-PAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLS 306
               YS     E+ FGG+  G++ L   FDSGSSYTYFNS+ Y AV  LLK +L GKPL 
Sbjct: 248 YSKHYSPAMGGELLFGGRTTGLKNLLTVFDSGSSYTYFNSKAYQAVTYLLKRELSGKPLK 307

Query: 307 DEPKDKTLPVCWKGTKTFRSVVDVRNFFKPLALSFTDS--KNVQFQIPPEAYLIISKFGN 366
           +   D TLP+CW+G + F S+ +V+ +FKPLALSF         F+IPPEAYLIIS  GN
Sbjct: 308 EARDDHTLPLCWQGRRPFMSIEEVKKYFKPLALSFKTGWRSKTLFEIPPEAYLIISMKGN 367

Query: 367 VCFGILNGSQVGLGNVNVIGDISLLDKIVVYDNERQQIGWAPANCN 404
           VC GILNG+++GL N+N+IGDIS+ D++++YDNE+Q IGW P +C+
Sbjct: 368 VCLGILNGTEIGLQNLNLIGDISMQDQMIIYDNEKQSIGWMPVDCD 412

[Close]

BLAST of MS004531 vs. TAIR 10
Match: AT4G33490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 382.9 bits (982), Expect = 3.3e-106
Identity = 193/380 (50.79%), Postives = 245/380 (64.47%), Query Frame = 0


Query: 7   FPVSILFAIFAVSFSDKFSLEDRMQSGSGS--NRFASSVAFPIKGNVYPLGHFTVSVNIG 66
           F + ++     + FS       R  +G      R  SSV FP+ GNVYPLG++ V++NIG
Sbjct: 5   FMIVLMVMSLVLGFSSAVDFRWRKTAGFSDRFTRAVSSVVFPVHGNVYPLGYYNVTINIG 64

Query: 67  NPPKIFELDIDTGSDLTWVQCDAPCTGCTLPRDKQYRPHSNAVRCAEPLCSALFFPGKVP 126
            PP+ + LD+DTGSDLTW+QCDAPC  C       Y+P S+ + C +PLC AL       
Sbjct: 65  QPPRPYYLDLDTGSDLTWLQCDAPCVRCLEAPHPLYQPSSDLIPCNDPLCKALHLNSNQR 124

Query: 127 CKNPNDQCDYDVEYADQGSSIGVLVRDLVPMRLTNGSVFAPNLGFGCGYDQKHGGST--- 186
           C+ P +QCDY+VEYAD GSS+GVLVRD+  M  T G    P L  GCGYDQ  G S+   
Sbjct: 125 CETP-EQCDYEVEYADGGSSLGVLVRDVFSMNYTQGLRLTPRLALGCGYDQIPGASSHHP 184

Query: 187 -AGVLGLGSGKATMTSQLSALGHVRNVVGHCLSGQGGGFLFFGGDLVPSSGMSWTPIMPS 246
             GVLGLG GK ++ SQL + G+V+NV+GHCLS  GGG LFFG DL  SS +SWTP+   
Sbjct: 185 LDGVLGLGRGKVSILSQLHSQGYVKNVIGHCLSSLGGGILFFGDDLYDSSRVSWTPMSRE 244

Query: 247 PGGRYSSG-PAEVYFGGKAAGIRGLTLTFDSGSSYTYFNSQVYGAVLNLLKNDLKGKPLS 306
               YS     E+ FGG+  G++ L   FDSGSSYTYFNS+ Y AV  LLK +L GKPL 
Sbjct: 245 YSKHYSPAMGGELLFGGRTTGLKNLLTVFDSGSSYTYFNSKAYQAVTYLLKRELSGKPLK 304

Query: 307 DEPKDKTLPVCWKGTKTFRSVVDVRNFFKPLALSFTDS--KNVQFQIPPEAYLIISKFGN 366
           +   D TLP+CW+G + F S+ +V+ +FKPLALSF         F+IPPEAYLIIS  GN
Sbjct: 305 EARDDHTLPLCWQGRRPFMSIEEVKKYFKPLALSFKTGWRSKTLFEIPPEAYLIISMKGN 364

Query: 367 VCFGILNGSQVGLGNVNVIG 378
           VC GILNG+++GL N+N+IG
Sbjct: 365 VCLGILNGTEIGLQNLNLIG 383

The following BLAST results are available for this feature:

BLAST of MS004531 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132194.1	1.6e-240	99.51	aspartic proteinase Asp1-like isoform X4 [Momordica charantia]	[more]
XP_022132187.1	5.4e-236	93.12	aspartic proteinase Asp1-like isoform X3 [Momordica charantia]	[more]
XP_022132180.1	4.6e-235	91.44	aspartic proteinase Asp1-like isoform X2 [Momordica charantia]	[more]
XP_022132155.1	1.5e-230	86.02	aspartic proteinase Asp1-like isoform X1 [Momordica charantia] >XP_022132164.1 a...	[more]
XP_004142705.1	1.6e-187	76.76	aspartic proteinase Asp1 [Cucumis sativus]	[more]

BLAST of MS004531 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0IU52	6.1e-81	43.49	Aspartic proteinase Asp1 OS=Oryza sativa subsp. japonica OX=39947 GN=ASP1 PE=2 S...	[more]
A2ZC67	4.3e-79	43.23	Aspartic proteinase Asp1 OS=Oryza sativa subsp. indica OX=39946 GN=ASP1 PE=2 SV=...	[more]
Q9M9A8	7.7e-76	40.20	Aspartyl protease APCB1 OS=Arabidopsis thaliana OX=3702 GN=APCB1 PE=1 SV=1	[more]
Q9S9K4	5.6e-34	28.93	Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2	[more]
Q4V3D2	2.6e-31	25.52	Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1	[more]

BLAST of MS004531 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BSD8	7.8e-241	99.51	aspartic proteinase Asp1-like isoform X4 OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1BT55	2.6e-236	93.12	aspartic proteinase Asp1-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1BRQ3	2.2e-235	91.44	aspartic proteinase Asp1-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1BT39	7.4e-231	86.02	aspartic proteinase Asp1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A0A0L366	7.7e-188	76.76	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G36185...	[more]

BLAST of MS004531 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G44130.1	1.1e-133	57.37	Eukaryotic aspartyl protease family protein	[more]
AT1G77480.2	6.8e-128	53.06	Eukaryotic aspartyl protease family protein	[more]
AT1G77480.1	2.6e-127	52.94	Eukaryotic aspartyl protease family protein	[more]
AT4G33490.2	6.4e-118	50.99	Eukaryotic aspartyl protease family protein	[more]
AT4G33490.1	3.3e-106	50.79	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 266..277 score: 27.34 coord: 374..389 score: 29.58 coord: 63..83 score: 46.07
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 9..405
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 266..398 e-value: 3.4E-14 score: 52.9
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 37..223 e-value: 7.7E-40 score: 138.9
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 226..404 e-value: 2.4E-28 score: 100.7
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 50..404
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 57..224 e-value: 7.8E-44 score: 150.0
None	No IPR available	PANTHER	PTHR13683:SF227	EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEIN	coord: 9..405
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 72..83
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 57..398 score: 34.671539

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS004531.1	MS004531.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity